Medical intestinal flora extraction device for medical examination

Abstract

The invention discloses a medical intestinal flora extraction device for medical examination in the technical field of flora extraction devices.The medical intestinal flora extraction device comprises a base, a controller is fixedly connected to the side face of the base, a supporting column is fixedly connected to the center of the top wall of the base, and the supporting column sequentially penetrates through and is fixedly connected with a lower-layer supporting disc and a middle-layer supporting disc from bottom to top; the top end of the supporting column is fixedly connected with a lifting rod, the top end of the lifting rod is slidably sleeved with a first sleeve, the top wall of the first sleeve is fixedly connected with an upper-layer supporting disc, the upper-layer supporting disc is provided with a sampling assembly, and the top wall of the lower-layer supporting disc and the top wall of the middle-layer supporting disc are provided with a plurality of corresponding dilution grooves and sampling grooves respectively. A sampling tube and a diluting tube are respectively arranged in the sampling tank and the diluting tank; and the sampling assembly is in signal connection with the controller. The device is stable and efficient, the sample on the cotton swab is fully suspended in the buffer solution by rotating and elastically oscillating the cotton swab, so that the sample is prevented from being scratched on the sampling tube, the treatment efficiency of the intestinal flora sample is improved, and the integrity of the intestinal microorganism sample is protected.

Description

Technical Field

[0001] The present invention belongs to the technical field of flora extraction devices, and specifically relates to a medical intestinal flora extraction device for medical examinations. Background Art

[0002] Intestinal microorganisms, especially the intestinal flora, are regarded as a special "organ" in the human body, and they are closely related to human health. Modern medicine has increasingly recognized the important role of intestinal bacteria in the human body, including participating in nutrient metabolism, maintaining immune balance, protecting the intestinal barrier, etc. Therefore, by deeply understanding the structure and function of the intestinal flora, exploring its association with health and diseases, and maintaining or reconstructing the normal intestinal flora in the human body have become important means for the treatment of various diseases.

[0003] In order to deeply study the relationship between intestinal microorganisms and human health, and to develop treatment means targeting intestinal microorganisms, it is necessary to extract intestinal flora from human samples. Usually, microbial DNA is extracted from fecal or intestinal tissue samples, and then the composition and function of the intestinal flora are analyzed. Currently, when hospitals extract intestinal microorganisms, most of them adopt the method of diluting feces and then statically filtering using a standard sampling sieve. However, this method has disadvantages such as being prone to blockage, long static extraction time, and being psychologically unacceptable to medical staff. There is also no stable base and integrated instrument for execution during the processing. When taking fecal samples, a cotton swab is needed to suspend the sample in a buffer solution, but due to the viscosity of the sample, the suspension effect is often not ideal, and it is easy to wipe the sample on the test tube wall, making it more difficult to suspend the sample, and the microbial sample is also lost accordingly. This process not only has a high operation difficulty, low suspension and filtration efficiency, but also easily causes discomfort to the operator.

[0004] Therefore, it is necessary to propose a medical intestinal flora extraction device for medical examinations that can perform radial rotation and longitudinal oscillation sampling on the cotton swab dipped in the sample through an automated mechanism, so that the sample on the cotton swab is fully suspended in the buffer solution, and can improve the filtration speed of the filter screen through the piston effect, making the sampling, suspension, and filtration effects in the intestinal microorganism extraction work more sufficient, with a shorter time requirement, thereby improving the sample quality and reduction degree of the intestinal microorganism extraction. Summary of the Invention

[0005] In order to solve the above problems, the purpose of the present invention is to provide a medical intestinal flora extraction device for medical examinations. By rotating and elastically oscillating the cotton swab clamped with the sample, the sample on the cotton swab is fully suspended in the buffer solution, avoiding scraping on the sampling tube, making the suspension effect more sufficient. At the same time, through the cooperation of the inner and outer tubes of the sampling tube, the piston movement is used to push the suspension liquid to pass through the filter screen faster for filtration, improving the processing efficiency of the intestinal flora sample, reducing the manpower requirement, and protecting the integrity of the intestinal microorganism sample.

[0006] To achieve the above object, the technical solution of the present invention is as follows: A medical intestinal flora extraction device for medical examination, including a base, a controller is fixedly connected to the side of the base, a support column is vertically and fixedly connected to the center of the top wall of the base, and the support column sequentially passes through and is fixedly connected to a lower support disk and a middle support disk from bottom to top. The top of the support column is fixedly connected to a lifting rod, a lifting nut is threadedly sleeved on the lifting rod, an axial lifting tooth is opened on the outer circumference of the lifting nut, a lifting motor facing upward is fixedly connected to the top wall of the middle support disk, a first gear is coaxially fixedly connected to the output shaft of the lifting motor, the first gear meshes with the lifting tooth, a first sleeve is coaxially rotatably connected to the top wall of the lifting nut, the first sleeve is slidably sleeved on the lifting rod and the top wall of the first sleeve is fixedly connected to an upper support disk, and a sampling assembly for rotating and shaking a cotton swab to separate the sample is provided on the upper support disk. A plurality of dilution grooves are opened on the top wall of the lower support disk, a plurality of sampling grooves corresponding to the dilution grooves are opened on the top wall of the middle support disk, sampling tubes are placed in the sampling grooves, dilution tubes are placed in the dilution grooves, and the lifting motor and the sampling assembly are both in signal connection with the controller.

[0007] The principle of the basic solution is: Clamp the cotton swab dipped with the sample by the sampling assembly, and then start the sampling motor through the controller. Through the rotation and elastic oscillation inside the sampling assembly, the sample on the cotton swab is fully suspended in the buffer solution. Then, the controller starts the lifting motor to drive the lifting nut and the upper support disk to move up and down, filter the suspension and introduce it into the dilution tube, and finally obtain a pure intestinal flora sample suspension.

[0008] The beneficial effects of the basic solution are: 1. The intestinal flora extraction device of the present invention can ensure that the intestinal microbial samples adhered to the cotton swab are fully and evenly suspended in the buffer solution through a unique rotation and elastic oscillation mechanism. This mechanism avoids the problems of sample damage or loss due to scraping in the traditional method, thus greatly improving the integrity and representativeness of the sample. At the same time, since the suspension process is carried out in a relatively closed and controlled environment, the risk of sample contamination by external factors is also reduced, further ensuring the quality of the sample.

[0009] 2. The built-in lifting motor and sampling assembly of the device can achieve precise automatic operation. From the rotation and oscillation of the cotton swab to the introduction and filtration of the suspension, almost no manual intervention is required during the whole process, significantly improving the processing efficiency. This automatic operation not only reduces the work burden of medical staff, but also reduces human errors, improving the accuracy and reliability of the test results.

[0010] 3. The design of the device fully considers the user's usage habits and convenience. Through the controller, various parameters such as rotation speed, oscillation intensity, and lifting height can be easily set and adjusted to meet different sample types and experimental requirements.

[0011] 4. The intestinal flora samples extracted by the device of the present invention can more truly reflect the actual composition and functional status of intestinal microorganisms because they avoid sample loss and contamination risks in traditional methods. This provides more reliable and accurate experimental materials and data support for subsequent research such as microbial analysis, disease diagnosis, and drug screening.

[0012] 5. The intestinal flora extraction device of the present invention is not only applicable to the field of medical testing, but also widely used in the research of multiple disciplinary fields such as intestinal microbiology, nutrition, and ecology. Its characteristics of high efficiency, accuracy, and automation will greatly promote the in-depth development of intestinal microbiology research and provide strong technical support for revealing the mystery between intestinal microorganisms and human health.

[0013] Furthermore, the sampling assembly includes a sampling motor at the center of the top wall of the upper support disk. The output shaft of the sampling motor is vertically downward and coaxially fixedly connected with a second gear. The second gear is located inside the upper support disk, and both the top wall and the bottom wall are slidably matched with the inner wall of the upper support disk. A number of through holes corresponding to the sampling slots are formed on the upper support disk. The top walls of the through holes are all coaxially slidably matched with gear rings. The second gear meshes with the gear rings. A clamping member is coaxially arranged inside each gear ring. A number of elastic bands are fixedly connected to the inner circumference of each gear ring. The outer circumference of the clamping member and the elastic bands in the same gear ring are fixedly connected. The top walls of the clamping members are all coaxially fixedly connected with second sleeves. A number of radially extending convex columns are fixedly connected to the outer circumference of the top wall of each second sleeve. A convex block protruding upward is fixedly connected to the outer circumference of each through hole. The convex blocks are all slidably matched with the corresponding convex columns. The sampling motor is signal-connected to the controller.

[0014] The beneficial effects of the basic solution are as follows: 1. The sampling assembly drives the second gear to rotate through the sampling motor, and then drives the gear rings, clamping members, and second sleeves meshing with it to rotate synchronously. Since the gear rings are slidably matched with the upper support disk, and the clamping members are fixedly connected to the gear rings through elastic bands, when the gear rings rotate, the clamping members will generate axial movement under the action of the collision between the convex columns on the outer circumference of the second sleeves and the convex blocks, and such movement is realized by the pulling of the elastic bands to achieve reciprocating oscillation. This design not only ensures the clamping stability of the cotton swab during rotation and oscillation, but also avoids sample loss caused by accidental scraping of the tube wall during manual operation of stirring the suspension sample in the traditional method.

[0015] 2. The design of the sampling assembly fully considers the compactness and easy maintenance of the structure. All key components are installed on the upper support disk through sliding fit and fixed connection, which not only ensures the stability and durability of the device, but also facilitates subsequent maintenance and repair work.

[0016] 3. Since the sampling component can achieve the clamping and stable rotational oscillation of the cotton swab without manual intervention, it can significantly improve the efficiency and safety of suspending the sample from the cotton swab into the buffer solution.

[0017] Furthermore, the clamping members each include a clamping disk fixedly connected to the elastic band within the same gear ring. A clamping hole is provided in the center of each clamping disk. A number of clamping arc blocks are provided inside the clamping hole. A third sleeve is rotatably connected coaxially to each clamping hole. An upper clamping ring is in threaded fit inside each third sleeve. A number of upper cranks extending obliquely downward are hinged to the inner wall of each upper clamping ring. A number of lower cranks extending obliquely upward corresponding to the upper cranks are hinged to the inner wall of the clamping hole. The middle parts of the upper cranks are cross-hinged with the middle parts of the corresponding lower cranks. The inner peripheral ends of the cross-hinged upper cranks and lower cranks are respectively hinged to the bottom end and the top end of the corresponding clamping arc block. The top wall height of each third sleeve exceeds that of the second sleeve. An anti-slip pattern is fixedly connected to the outer periphery of the top of each third sleeve.

[0018] The beneficial effects of the basic solution are as follows: 1. Through the interaction of the clamping disk, clamping arc block, third sleeve, upper clamping ring, upper crank, and lower crank in the design of the clamping member, flexible and stable clamping of the cotton swab or other samples is achieved. When the third sleeve rotates, the upper clamping ring will move up and down accordingly, and then through the cross-hinged action of the upper crank and the lower crank, the clamping arc block is driven to perform radial expansion or contraction movement within the clamping hole. This design not only ensures the stability of the sample during clamping but also allows for fine-tuning according to the size and shape of the sample to adapt to different types of samples.

[0019] 2. By installing the upper clamping ring inside the third sleeve in a threaded fit manner, the position of the upper clamping ring can be conveniently adjusted, thereby changing the cross angle of the upper crank and the lower crank, and then adjusting the clamping force of the clamping arc block. This design enables the clamping member to adapt to samples of different hardnesses and sizes, ensuring that the sample will not fall off or be damaged due to insufficient clamping force during rotation and oscillation.

[0020] 3. The anti-slip pattern fixedly connected to the outer periphery of the top of the third sleeve not only improves the user's feel when rotating the third sleeve but also prevents operation errors caused by hand slippage. This design makes it easier, more accurate, and safer for the user to adjust the clamping force and clamp the sample.

[0021] 4. The design of the clamping member fully considers the requirements of sample extraction. By precisely controlling the expansion and contraction of the clamping arc block, it can ensure that the cotton swab makes full contact with the buffer solution during the rotation and oscillation at a fixed height, thereby improving the suspension effect and extraction efficiency of the sample.

[0022] Furthermore, on one side of the sampling groove close to the outer periphery of the middle-layer support disc, pipe through grooves are opened. On the inner wall of one side of the sampling groove close to the support column, insertion blocks are fixedly connected axially. On the bottom wall of the sampling groove, groove pressure sensors are laid. The sampling pipes all include an outer pipe and an inner pipe that are slidably matched. The inner pipes are all fixedly connected with buckles. The bottom of the inner pipe is in an inverted frustum shape. Inside the outer pipes, silica gel valves are fixedly connected. On the inner wall of the outer pipes, filters are fixedly connected below the silica gel valves. On one side of the bottom of the outer pipe, a liquid guide pipe that is slidably matched with the pipe through groove is communicated. On one side of the outer wall of the outer pipe far from the liquid guide pipe, pipe insertion slots that are slidably matched with the insertion blocks are opened. The groove pressure sensors are all in signal connection with the controller.

[0023] The beneficial effects of the basic solution are as follows: 1. The design of the sampling groove on the middle-layer support disc, especially the pipe through groove close to the outer periphery and the insertion block far from the outer periphery, provides convenience for the installation of the sampling pipe and the derivation of liquid. Through the slidably matched liquid guide pipe and insertion block, the sampling pipe can be stably and directionally installed in the sampling groove, ensuring that the liquid can flow smoothly from the sampling pipe to the lower dilution groove. This design not only improves the efficiency of sample collection but also reduces sample loss caused by improper operation.

[0024] 2. The design of the sampling pipe combines the slidably matched outer pipe and inner pipe, as well as the inverted frustum shape design at the bottom of the inner pipe, enabling the sample to enter the outer pipe from the inner pipe conveniently and controllably for sample filtration, which can remove impurities and particulate matters in the sample, thereby improving the purity and quality of the sample.

[0025] 3. The groove pressure sensors laid on the bottom wall of the sampling groove can monitor the pressure change in the sampling groove in real time, thereby reflecting the state of the sample during the sampling process. Especially after suspension, the cotton swab is driven by the lifting motor to squeeze the inner pipe so that the inverted frustum-shaped bottom breaks through the silica gel valve, and the cotton swab is rotated and lifted to allow the suspension to fully flow onto the filter screen. After the liquid flows down, the cotton swab is lowered again to block the bottom of the inner pipe to form a piston. The inner pipe is repeatedly lifted and lowered to make the filtration process of the suspension faster and smoother. The operation and completion of these processes can be detected by the groove pressure sensors at the operation nodes. As a quality control detector for rapid filtration, it ensures the smooth realization of the automated rapid filtration process and improves the working quality of the device.

[0026] 4. The design of the disposable sampling pipe takes into account the convenience of user operation and the simplicity of maintenance. By using a non-reusable sampling pipe, sample contamination by other impurities and external microorganisms can be avoided during sample collection and release. At the same time, the slidably matched design of the liquid guide pipe and insertion block with the sampling groove enables the sampling pipe to be conveniently installed and disassembled, facilitating replacement and positioning.

[0027] Furthermore, the through hole corresponds coaxially to the sampling groove, and the center of the dilution groove corresponds to the end of the liquid guide pipe.

[0028] The beneficial effects of the basic solution are as follows: 1. The coaxial correspondence design between the through-hole and the sampling groove ensures the high efficiency and accuracy of the cotton swab rotating and oscillating to suspend the sample. When the sampling tube is inserted into the sampling groove, its inner tube can be coaxially corresponded with the cotton swab clamped in the through-hole, reducing the probability of sample loss due to the scraping between the cotton swab and the inner tube wall. This design not only improves the suspension efficiency of the sample but also reduces the loss of the sample during the suspension sampling process.

[0029] 2. The corresponding design between the center of the dilution groove and the end of the liquid guiding tube ensures the smoothness of liquid flow. When the sample is led out from the sampling tube through the liquid guiding tube, it can directly enter the central position of the dilution tube in the dilution groove, avoiding the liquid from sticking to the wall or splashing inside the dilution tube. This design helps to achieve uniform dilution of the sample and improves the accuracy and reliability of subsequent experiments or analyses.

[0030] 3. This design also simplifies the operation process and reduces the operation steps of the user during the sample transfer and dilution process. The user only needs to insert the sampling tube into the sampling groove and then introduce the sample suspension into the dilution tube through the liquid guiding tube, without the need for additional adjustment or alignment. This design not only improves the operation efficiency but also reduces the risk of errors caused by improper operation.

[0031] Furthermore, a plurality of laser indicators respectively facing the centers of the corresponding through-holes are fixedly connected to the outer peripheral top wall of the upper support plate, and the laser indicators are all signal-connected to the controller.

[0032] The beneficial effects of the basic solution are as follows: 1. The laser indicator can emit a laser beam and accurately point to the equal height position above each through-hole. This enables the sampling operator to clearly see the clamping height that each cotton swab should have, thus ensuring the uniformity and accuracy of the suspension sampling process. The ordinary clamping method relies on the operator's experience and visual judgment, which is prone to introducing human errors. The use of the laser indicator greatly reduces such errors and improves the accuracy and reliability of sampling.

[0033] 2. The laser indicator makes the clamping operation more intuitive and fast. The operator only needs to quickly find and locate the clamping height of each cotton swab according to the guidance of the laser beam, thus greatly shortening the time required for clamping. In the ordinary clamping method, the staff may need to spend a lot of time adjusting the position and angle of the clamping tool. The use of the laser indicator reduces this adjustment time and makes the sampling process more efficient.

[0034] 3. All the laser indicators are signal-connected to the controller, which enables the laser indicators to realize other indication functions and improves the convenience and accuracy of control. The normal lighting or flashing of a single laser indicator can prompt situations such as faults and operation mistakes, facilitating the user to adjust the device in time and avoiding sample damage.

[0035] Furthermore, friction layers and clamping pressure sensors are laid on the sides of the clamping arc blocks away from the clamping holes, and the clamping pressure sensors are all connected to the controller in signal.

[0036] The beneficial effects of the basic solution are as follows: 1. The laying of the friction layer increases the contact area and friction force between the clamping arc block and the object to be clamped, thereby improving the clamping stability. This design helps to prevent the cotton swab from falling off or moving under the action of rotational oscillation.

[0037] 2. By monitoring the clamping pressure in real time, safety accidents caused by misoperation can be avoided. For example, when the clamping pressure exceeds the set range, the controller can automatically stop the rotational oscillation of the cotton swab and send out an alarm signal to remind the user to deal with it in time to avoid the loss of samples.

[0038] Furthermore, a vacuum pump is fixedly connected inside the base. The output end of the vacuum pump communicates with the top wall of the base, and the input end of the vacuum pump communicates with the side wall of the base. A protective shell made of transparent material is detachably connected to the top wall of the base, and the vacuum pump is connected to the controller in signal.

[0039] The beneficial effects of the basic solution are as follows: 1. Since some of the intestinal flora microorganisms are anaerobic bacteria, when it is necessary to specifically extract these microorganisms, deoxygenation is required to ensure the survival of anaerobic bacteria. By cooperating with the protective shell, the vacuum pump can create an environment with a lower oxygen content inside the protective shell to avoid the loss of anaerobic bacteria samples.

[0040] 2. At the same time, the environment with thin air formed by the vacuum pump inside the protective shell is also beneficial to the movement of each structure inside the device, reducing air resistance, optimizing the accuracy of movement, and improving the extraction efficiency and quality of intestinal flora microorganisms.

[0041] 3. The protective shell made of transparent material enables the operator to directly observe the working state of the device inside the base, as well as possible impurities or abnormal conditions. This real-time monitoring ability helps the operator to promptly discover and handle potential problems, ensuring the stable operation of the equipment. Since the protective shell is transparent, the operator can observe the internal situation without disassembling the protective shell, thus simplifying the operation process, improving work efficiency, and reducing the risk of equipment wear and failure caused by frequent disassembly and installation of the protective shell. The transparent protective shell can visually display the impurities and dirt accumulated inside the protective shell, thus simplifying the cleaning process. The operator only needs to select appropriate cleaning methods and tools according to the observed dirt conditions to quickly and effectively clean the inside of the protective shell and keep the equipment clean and hygienic.

[0042] Furthermore, airtight rubber strips are laid at the corresponding positions of the top wall of the base and the bottom wall of the protective shell.

[0043] The beneficial effects of the basic solution are as follows: The airtight rubber strip is mainly used to ensure the airtightness between the protective shell and the base, facilitating the vacuum pump to create a suspension environment with less oxygen content. It also prevents external pollutants such as dust and microorganisms from being inhaled into the protective shell and affecting the extraction accuracy of intestinal flora microorganisms.

[0044] Furthermore, a temperature control device is provided inside the base to lower the temperature inside the protective shell. The temperature control device is signal-connected to the controller.

[0045] The beneficial effects of the basic solution are as follows: The temperature control device lowers the temperature inside the protective shell for the extraction of intestinal microbiota. On the one hand, it can reduce the death of sample strains, making the extraction integrity better, which is beneficial for the subsequent detection steps. On the other hand, it can reduce the heat generated by the movement friction of the device during the extraction process, prevent the metal from overheating and fatiguing, and extend the service life of the device. Description of the Drawings

[0046] Figure 1 Isometric view of the medical intestinal flora extraction device for medical inspection in the embodiment of the present invention;

[0047] Figure 2 Lateral sectional view of the medical intestinal flora extraction device for medical inspection in the embodiment of the present invention;

[0048] Figure 3 Front sectional view of the medical intestinal flora extraction device for medical inspection in the embodiment of the present invention;

[0049] Figure 4 Side view of the medical intestinal flora extraction device for medical inspection in the embodiment of the present invention;

[0050] Figure 5 Top view of the medical intestinal flora extraction device for medical inspection in the embodiment of the present invention;

[0051] Figure 6 Enlarged view of A in the medical intestinal flora extraction device for medical inspection in the embodiment of the present invention.

[0052] The reference numerals in the drawings of the specification include: 1, base; 2, controller; 3, airtight rubber strip; 4, lower support plate; 5, dilution tube; 6, middle support plate; 7, liquid guide tube; 8, outer tube; 9, inner tube; 10, upper support plate; 11, laser indicator; 12, anti-slip pattern; 13, third sleeve; 14, second sleeve; 15, convex column; 16, convex block; 17, lifting nut; 18, buckle cover; 19, first gear; 20, lifting motor; 21, support column; 22, vacuum pump; 23, second gear; 24, sampling motor; 25, first sleeve; 26, lifting rod; 27, tank pressure sensor; 28, filter screen; 29, silica gel valve; 30, pipe slot; 31, insertion block; 32, temperature control component; 33, gear ring; 34, elastic band; 35, upper clamping ring; 36, lower crank; 37, clamping arc block; 38, lower clamping ring; 39, clamping plate; 40, clamping hole; 41, pipe through slot. Detailed Description of the Specific Embodiment

[0053] The following is a further detailed description through specific embodiments:

[0054] Embodiment 1:

[0055] Basically as shown in the attached Figure 1 , Figure 2 , Figure 3 , Figure 4 , Figure 5 and Figure 6Shown: A medical intestinal flora extraction device for medical tests, including a base 1, a controller 2 is welded to the side of the base 1, a support column 21 is vertically welded to the center of the top wall of the base 1, the support column 21 sequentially passes through and is welded with a lower support disk 4 and a middle support disk 6 from bottom to top, a lifting rod 26 is welded to the top of the support column 21, a lifting nut 17 is threadedly sleeved on the lifting rod 26, an axial lifting tooth is opened on the outer circumference of the lifting nut 17, a lifting motor 20 facing upward is adhesively bonded to the top wall of the middle support disk 6, a first gear 19 is coaxially welded to the output shaft of the lifting motor 20, the first gear 19 meshes with the lifting tooth, a first sleeve 25 is rotatably connected to the top wall of the lifting nut 17 by a pin, the first sleeve 25 is slidably sleeved on the lifting rod 26 and a top layer support disk 10 is welded to the top wall of the first sleeve 25, a sampling assembly for rotating and jittering a cotton swab to separate the sample is provided on the top layer support disk 10, a plurality of dilution grooves are opened on the top wall of the lower support disk 4, a plurality of sampling grooves corresponding to the dilution grooves are opened on the top wall of the middle support disk 6, sampling tubes are placed in the sampling grooves, dilution tubes 5 are placed in the dilution grooves, a vacuum pump 22 is installed inside the base 1, the output end of the vacuum pump 22 communicates with the top wall of the base 1, the input end of the vacuum pump 22 communicates with the side wall of the base 1, a protective shell made of a transparent material is detachably connected to the top wall of the base 1, airtight rubber strips 3 are laid at the corresponding positions of the top wall of the base 1 and the bottom wall of the protective shell, a semiconductor refrigeration sheet is also installed inside the base 1 as a temperature control component 32 for reducing the temperature inside the protective shell, the temperature control component 32, the vacuum pump 22 and the lifting motor 20 are all signal-connected to the controller 2.

[0056] The sampling assembly includes a sampling motor 24 adhesively bonded to the center of the top wall of the top layer support disk 10, the output shaft of the sampling motor 24 is vertically downward and coaxially welded with a second gear 23, the second gear 23 is located inside the top layer support disk 10 and both the top wall and the bottom wall are slidably matched with the inner wall of the top layer support disk 10, a plurality of through holes corresponding to the sampling grooves coaxially are opened on the top layer support disk 10, gear rings 33 are slidably matched with the top walls of the through holes coaxially, the second gear 23 meshes with the gear rings 33, clamping members are adhesively bonded coaxially inside the gear rings 33, a plurality of elastic bands 34 are adhesively bonded to the inner circumferences of the gear rings 33, the outer circumference of the clamping member and the elastic band inside the same gear ring 33 are adhesively bonded, second sleeves 14 are coaxially welded to the top walls of the clamping members, a plurality of radially extending convex columns 15 are welded to the outer circumference of the top wall of the second sleeve 14, convex blocks 16 protruding upward are welded to the outer circumference of the through holes, the convex blocks 16 are slidably matched with the corresponding convex columns 15, and the sampling motor 24 is signal-connected to the controller 2.

[0057] A plurality of laser indicators 11 respectively facing the centers of the corresponding through holes are welded to the outer circumferential top wall of the top layer support disk 10, and the laser indicators 11 are all signal-connected to the controller 2.

[0058] The specific implementation process is as follows: In the traditional intestinal microbial flora extraction operation, medical staff need to use a cotton swab to dip the stool sample, and then stir the cotton swab in the buffer solution to fully disperse and suspend the sample. This process not only makes it easy for the sample on the cotton swab to accidentally scrape onto the wall of the sampling tube, making it more difficult to disperse the sample into the buffer solution, but also requires the operator to have good stirring skills and strength. Otherwise, it will cause insufficient suspension, and the sample will be lost in the subsequent process of filtering impurities and diluting the sample, and even the filter 28 will be blocked, thereby reducing the extraction effect of the intestinal microbial flora and affecting the accuracy of intestinal microbial identification after extraction.

[0059] The device is designed to solve these problems. After the medical staff uses a cotton swab to pick up the sample, they directly pass the cotton swab sample downward from bottom to top through the clamping piece on the upper support plate 10, and use the laser pointer 11 to point to the center of the clamping hole 40 to indicate the standard height of the cotton swab. Figure 2 As shown, the requirements for the operator's extraction experience in the cotton swab fixing operation are reduced, the convenience of the extraction process is improved, and after the cotton swab reaches a predetermined height under the cotton swab height indication, the cotton swab is clamped at a fixed height by the clamping member.

[0060] After the cotton swab is clamped, a sampling tube is inserted into the sampling slot on the middle support plate 6 under the corresponding cotton swab, and an appropriate amount of buffer solution with added antioxidant is added to the sampling tube and the dilution tube 5. The above is the entire manual operation process of the device. After completing these operations, the device can be turned on by the controller 2 to separate the sample stained on the cotton swab and fully stir and suspend it, filter the impurities in the suspension, and guide the suspension to prepare for dilution.

[0061] Considering that some anaerobic bacteria exist in the intestinal microbial flora samples, in order to fully maintain the integrity of the flora in the sample suspension, Figure 1 As shown, first, a protective shell is put on the base 1 to seal it, and a vacuum pump 22 is used to extract part of the air in the protective shell to reduce the oxygen content and avoid the death of anaerobic bacteria. The airtight rubber strip 3 can ensure the stable difference between the internal and external air pressures and reduce the air resistance during the internal mechanical movement. At the same time, the semiconductor refrigeration chip as the temperature control unit 32 is turned on to reduce the temperature in the protective shell. Figure 2As shown, the lifting motor 20 is then turned on by the controller 2. The rotation of the lifting motor 20 drives the first gear 19 and the engaged lifting nut 17 to rotate. Due to the threads on the surface of the lifting rod 26, the rotating lifting nut 17 descends on the lifting rod 26. When the first sleeve 25 and the upper support plate 10 descend on the lifting rod 26 by a suitable distance under the influence of their own weights, the equally high cotton swabs being clamped are immersed in the buffer solution added to the sampling tube. Then, the sampling motor 24 is turned on to rotate the second gear 23, and the second gear 23 drives all the gear rings 33 engaged with it to rotate coaxially around the through hole. During the rotation of the gear ring 33, the clamping member and the stably clamped cotton swab are also driven by the elastic band 34 to rotate inside the gear ring 33. The sample at the bottom of the cotton swab generates centrifugal force and disperses in the buffer solution. Since the current motion state of the cotton swab is also coaxial rotation, the sample on the surface of the cotton swab always stays away from the sampling tube wall, reducing the risk of the sample on the cotton swab scraping against the tube wall; on this basis, the second sleeve 14 on the clamping member rotates with the clamping member, and the convex posts 15 on the top wall of the second sleeve 14 continuously cooperate with the symmetrical convex blocks during rotation to continuously lift and lower the clamping member and the cotton swab clamped thereon through the second sleeve 14. Due to the pulling and energy storage effect of the elastic band 34 on the clamping member, even after the convex posts 15 are reset, the clamping member and the cotton swab will still elastically oscillate in the plane of the gear ring 33, thereby applying a vertical reciprocating motion and oscillation effect to the sample at the bottom of the cotton swab. This motion mode helps the sample to oscillate and disperse in the buffer solution, supplementing the all-round dispersion effect of the sample, enabling the sample to be fully and evenly dispersed in the buffer solution, and further improving the integrity and detection accuracy of the subsequent intestinal microbiota.

[0062] Example 2:

[0063] The difference from the above embodiment is that, as Figure 3 and Figure 6 shown: The clamping members all include clamping disks 39 adhesively bonded to the elastic band 34 inside the same gear ring 33. Clamping holes 40 are provided in the centers of the clamping disks 39. A number of clamping arc blocks 37 are hinged inside the clamping holes 40. Third sleeves 13 are coaxially rotatably connected to the clamping holes 40. Upper clamping rings 35 are in threaded cooperation inside the third sleeves 13. A number of upper cranks extending obliquely downward are hinged to the inner walls of the upper clamping rings 35. A number of lower cranks 36 extending obliquely upward corresponding to the upper cranks are hinged to the inner walls of the clamping holes 40. The middle parts of the upper cranks are cross-hinged with the middle parts of the corresponding lower cranks 36. The inner peripheral ends of the cross-hinged upper cranks and lower cranks 36 are respectively hinged to the bottom ends and top ends of the corresponding clamping arc blocks 37. Friction layers and clamping pressure sensors are provided on the sides of the clamping arc blocks 37 away from the clamping holes 40. The clamping pressure sensors are all in signal connection with the controller 2. The top wall heights of the third sleeves 13 all exceed those of the second sleeves 14, and anti-slip threads 12 are provided on the outer circumferences of the tops of the third sleeves 13.

[0064] The specific implementation process is as follows: After the cotton swab reaches the preset height, as Figure 6 shown, the operator easily rotates the third sleeve 13 with anti-slip threads 12, so that the upper clamping ring 35 inside the third sleeve 13 is stably pushed downward by the thread and approaches the lower clamping ring 38. The upper and lower cranks 36 hinged on the upper and lower clamping rings 35 radially extend and push a plurality of clamping arc blocks 37 to move towards the center of the clamping hole 40. The clamping force of the clamping arc blocks 37 and the friction layer on their surfaces can firmly clamp the cotton swab at a fixed height, avoiding sample loss caused by the cotton swab loosening and falling off during rotation and oscillation. The clamping pressure sensor is the second insurance for the safety of the sampling process. When the cotton swab is loosely clamped, it is detected by the clamping pressure sensor and fed back to the controller 2, and the sampling is paused in time and the operator is reported to make adjustments to reduce the possibility of further sample loss and device damage.

[0065] Embodiment 3:

[0066] The difference from the above embodiment is that, as Figure 3 and Figure 4 shown: Pipe through grooves 41 are opened on one side of the sampling groove close to the outer periphery of the middle layer support disk 6. Insert blocks 31 are axially bonded to the inner walls of one side of the sampling groove close to the support column 21. Groove pressure sensors 27 are laid on the bottom walls of the sampling grooves. The sampling pipes all include an outer pipe 8 and an inner pipe 9 that are slidably matched. The inner pipes 9 are integrally formed with buckle covers 18. The bottoms of the inner pipes 9 are all in the shape of an inverted frustum of a cone. Silicone valves 29 are bonded inside the outer pipes 8. Filter meshes 28 are bonded to the inner walls of the outer pipes 8 below the silicone valves 29. One side of the bottom of the outer pipes 8 is all communicated with a liquid guide pipe 7 that is slidably matched with the pipe through groove 41. The end of the liquid guide pipe 7 corresponds to the center of the dilution pipe 5. Pipe insertion slots 30 that are slidably matched with the insert blocks 31 are opened on one side of the outer wall of the outer pipe 8 away from the liquid guide pipe 7. The groove pressure sensors 27 are all in signal connection with the controller 2.

[0067] The specific implementation process is as follows: When installing the sampling pipe in the sampling groove, pass the liquid guide pipe 7 on the sampling pipe through the pipe through groove 41, insert the insert block 31 on the other side of the sampling pipe into the pipe insertion slot 30, quickly and stably insert the sampling pipe into the sampling groove to the bottom and align the liquid guide pipe 7 with the dilution groove, and then place the dilution pipe 5 in the corresponding dilution groove. At this time, the silicone valve 29 in the outer pipe 8 is in a closed state, controlling the buffer liquid in the inner pipe 9 to be difficult to flow into the outer pipe 8.

[0068] After the sampling motor 24 finishes running and the sample is fully dispersed in the buffer solution, it is necessary to filter the impurities in the sample dispersion. At this time, the controller 2 controls the lifting motor 20 to restart, rotates the first gear 19 and the lifting nut 17 rotates, and the lifting nut 17 continues to descend. The self-weight of the upper support plate 10 drives the clamping cotton swab to move downward to reach the bottom of the inner tube 9 of the sampling tube, and pushes the bottom of the inverted conical table of the inner tube 9 to break through the sealing effect of the silicone valve 29 of the outer tube 8. At the same time, the sampling motor 24 is started to rotate to loosen and lift the cotton swab, and the sample dispersion flows from the inner tube 9 onto the filter screen 28 under the silicone valve 29. Since the filter holes of the filter screen 28 are relatively small, there is still a possibility of being blocked by the impurities in the sample dispersion. At this time, after the sample dispersion flows out of the inner tube 9, the cotton swab can be lowered again to block the bottom of the inner tube 9 to form a simple piston in the outer tube 8. By driving the lifting and falling of the outer tube 8 with the cotton swab, the sample dispersion on the filter screen 28 can pass through the filter screen 28 faster, accelerating the sampling fluency and efficiency.

[0069] As Figure 1 shown, the sample dispersion flowing through the filter screen 28 is led out from the outside of the middle support plate 6 through the liquid guide tube 7 on the side of the bottom of the outer tube 8 and falls into the dilution tube 5 in the dilution tank of the lower support plate 4. Then, the operator can control the vacuum pump 22 through the controller 2 to balance the air pressure inside and outside the protective shell, take out the dilution tube 5 and perform the next dilution step on ice to obtain a sample dispersion with full dispersion and intact intestinal microbial flora, improving the accuracy and efficiency of subsequent extraction and detection.

[0070] Since both the sampling tube and the dilution tube 5 are disposable items, the cleaning process after extraction is very simple. The sample will not come into contact with other components. It only needs to replace the sampling tube and the dilution tube 5 and simply disinfect the clamping parts and other parts, simplifying the cleaning process of the sampling steps and improving the sustainability of the sampling function of this device.

[0071] It should be noted that in this article, relational terms such as first and second are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations. Moreover, the term "including", "comprising" or any other variant thereof is intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements not only includes those elements, but also includes other elements not expressly listed, or also includes elements inherent to such process, method, article or device.

[0072] The above are only embodiments of the present invention. Specific structures and common knowledge such as characteristics well-known in the art are not described in detail herein. Those of ordinary skill in the art know all the common general technical knowledge in the technical field to which the invention pertains before the filing date or the priority date, can learn all the prior arts in this field, and have the ability to apply the conventional experimental means before this date. Those of ordinary skill in the art can, under the inspiration given in this application, combine their own abilities to complete and implement this solution. Some typical well-known structures or well-known methods should not become obstacles for those of ordinary skill in the art to implement this application. It should be noted that for those skilled in the art, without departing from the structure of the present invention, several deformations and improvements can be made, which should also be regarded as the protection scope of the present invention, and these will not affect the implementation effect of the present invention and the practicality of the patent. The protection scope claimed in this application should be based on the content of its claims, and the specific implementation manners described in the specification can be used to interpret the content of the claims.

Claims

1. A medical intestinal flora extraction device for medical examination, comprising a base (1), characterized in that: A controller (2) is fixedly connected to the side of the base (1); a support column (21) is vertically fixedly connected to the center of the top wall of the base (1); the support column (21) passes through and is fixedly connected to the lower support plate (4) and the middle support plate (6) in sequence from bottom to top; a lifting rod (26) is fixedly connected to the top of the support column (21); a lifting nut (17) is threadedly sleeved on the lifting rod (26); an axial lifting tooth is formed on the outer periphery of the lifting nut (17); a lifting motor (20) facing upward is fixedly connected to the top wall of the middle support plate (6); an output shaft of the lifting motor (20) is coaxially fixedly connected to a first gear (19); the first gear (19 ) is meshed with the lifting teeth, the top wall of the lifting nut (17) is coaxially connected to the first sleeve (25), the first sleeve (25) is slidably sleeved with the lifting rod (26) and the top wall of the first sleeve (25) is fixedly connected to the upper support plate (10), the upper support plate (10) is provided with a sampling component for rotating and shaking the cotton swab to separate the sample, the top wall of the lower support plate (4) is provided with a plurality of dilution slots, the top wall of the middle support plate (6) is provided with a plurality of sampling slots corresponding to the dilution slots, the sampling slots are all provided with sampling tubes, the dilution slots are all provided with dilution tubes (5), the lifting motor (20) and the sampling component are both connected to the controller (2) by signal.

2. The medical intestinal flora extraction device for medical examination according to claim 1, characterized in that: The sampling assembly comprises a sampling motor (24) fixedly connected to the center of the top wall of the upper support plate (10); the output shaft of the sampling motor (24) is vertically downward and coaxially fixedly connected with a second gear (23); the second gear (23) is located inside the upper support plate (10) and the top wall and the bottom wall are both slidably matched with the inner wall of the upper support plate (10); the upper support plate (10) is provided with a plurality of through holes coaxially corresponding to the sampling slots; the top walls of the through holes are coaxially slidably matched with a gear ring (33); the second gear (23) is meshed with the gear ring (33); the gear ring (33) A clamping piece is coaxially provided inside, a plurality of elastic bands (34) are fixedly connected to the inner periphery of the gear ring (33), the outer periphery of the clamping piece in the same gear ring (33) is fixedly connected to the elastic band (34), the top wall of the clamping piece is coaxially fixedly connected to the second sleeve (14), the outer periphery of the top wall of the second sleeve (14) is fixedly connected to a plurality of radially extending convex columns (15), the outer periphery of the through hole is fixedly connected to symmetrical upwardly protruding convex blocks (16), the convex blocks (16) are slidably matched with the corresponding convex columns (15), and the sampling motor (24) is connected to the controller (2) by signal.

3. The medical intestinal flora extraction device for medical examination according to claim 2, characterized in that: The clamping parts all include a clamping plate (39) fixedly connected to the elastic band (34) in the same gear ring (33), a clamping hole (40) is opened in the center of the clamping plate (39), a plurality of clamping arc blocks (37) are arranged inside the clamping hole (40), a third sleeve (13) is coaxially rotatably connected to the clamping hole (40), an upper clamping ring (35) is threadedly matched inside the third sleeve (13), and a plurality of upper curved blocks (37) extending obliquely downward are hinged on the inner wall of the upper clamping ring (35). A plurality of lower cranks (36) corresponding to the upper cranks and extending obliquely upward are hinged on the inner wall of the clamping hole (40); the middle parts of the upper cranks are cross-hinged with the middle parts of the corresponding lower cranks (36); the inner peripheral ends of the cross-hinged upper cranks and lower cranks (36) are respectively hinged with the bottom and top ends of the corresponding clamping arc blocks (37); the top wall height of the third sleeve (13) exceeds that of the second sleeve (14); and the top periphery of the third sleeve (13) is fixedly connected with anti-slip grooves (12).

4. The medical intestinal flora extraction device for medical examination according to claim 1, characterized in that: A tube through groove (41) is provided on one side of the sampling groove close to the outer periphery of the middle support plate (6); an insert block (31) is axially fixedly connected to the inner wall of the sampling groove close to the support column (21); a groove pressure sensor (27) is laid on the inner bottom wall of the sampling groove; the sampling tubes include an outer tube (8) and an inner tube (9) that are slidably matched; the inner tube (9) is fixedly connected to a buckle cover (18); the bottom of the inner tube (9) is in an inverted frustum shape; a silicone valve (29) is fixedly connected inside the outer tube (8); a filter screen (28) located below the silicone valve (29) is fixedly connected to the inner wall of the outer tube (8); a liquid guide tube (7) that is slidably matched with the tube through groove (41) is connected to one side of the bottom of the outer tube (8); a tube slot (30) that is slidably matched with the insert block (31) is provided on the outer wall of the outer tube (8) that is away from the liquid guide tube (7); and the groove pressure sensor (27) is connected to the controller (2) for signal.

5. The medical intestinal flora extraction device for medical examination according to claim 1, characterized in that: The through hole is coaxially corresponding to the sampling slot, and the center of the dilution slot is corresponding to the end of the liquid guiding tube (7).

6. The medical intestinal flora extraction device for medical examination according to claim 1, characterized in that: A plurality of laser indicators (11) facing the centers of corresponding through holes are fixedly connected to the outer peripheral top wall of the upper support plate (10), and the laser indicators (11) are all connected to the controller (2) by signal.

7. The medical intestinal flora extraction device for medical examination according to claim 3, characterized in that: A friction layer and a clamping pressure sensor are provided on one side of the clamping arc block (37) away from the clamping hole (40), and the clamping pressure sensor is connected to the controller (2) by signal.

8. The medical intestinal flora extraction device for medical examination according to claim 1, characterized in that: A vacuum pump (22) is fixedly connected inside the base (1); an output end of the vacuum pump (22) is connected to a top wall of the base (1); an input end of the vacuum pump (22) is connected to a side wall of the base (1); a protective shell made of a transparent material is detachably connected to the top wall of the base (1); and the vacuum pump (22) is connected to a controller (2) via a signal.

9. The medical intestinal flora extraction device for medical examination according to claim 8, characterized in that: Airtight rubber strips (3) are laid at corresponding positions of the top wall of the base (1) and the bottom wall of the protective shell.

10. The medical intestinal flora extraction device for medical examination according to claim 8, characterized in that: A temperature control unit (32) is also provided inside the base (1) for reducing the temperature inside the protective shell. The temperature control unit (32) is connected to the controller (2) by signal.

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