Tumor cell cluster sorting and filtering device for oncology department

Abstract

The invention discloses a tumor cell cluster sorting and filtering device for the oncology department, and belongs to the technical field of tumor cell cluster treatment. Comprising a bottom pipe base, a flow storage pipe column, a filtering unit, a scrubbing unit, an interception filtering unit, an air pressure unit and a collecting tank, the two ends of the bottom pipe base are hollow, a partition plate is integrally manufactured in the middle of the bottom pipe base, and a grating groove is formed in the position, above the partition plate, of the bottom pipe base; a pipe penetrating hole and an exhaust hole are formed in the middle of the partition plate; the bottom of the flow storage pipe column is screwed with the top surface of the bottom pipe seat; a convex flow guide hole is formed in the bottom of the flow storage tubular column; a top cover is fixed at the top of the flow storage tubular column; a liquid inlet valve is arranged in the middle of the top cover; a plurality of flow channels are formed in the inner wall of the flow storage tubular column; according to the tumor cell cluster sorting and filtering device for the oncology department, the tumor cell clusters can be fully extracted from dispersion liquid, the dispersion and filtering efficiency of the tumor cell clusters is guaranteed, and the extracted tumor cell clusters can be protected.

Description

Technical Field

[0001] The present invention specifically relates to a sorting and filtering device for tumor cell clusters used in oncology, belonging to the technical field of tumor cell cluster processing. Background Art

[0002] During the work process, doctors in the oncology department often need to sort and filter tumor cell clusters through a sorting and filtering device, that is, adding fresh tissue dispersion liquid to the sorting and filtering device to sort and filter tumor cell clusters; since the sizes of larger tissues such as adipose tissue, membrane tissue, and connective tissue in the tissue dispersion liquid are hundreds of micrometers, while the sizes of tumor cell clusters in the tissue dispersion liquid are generally dozens of micrometers, therefore, the larger tissues often block the filter plate in the sorting and filtering device, affecting the sorting of tumor cell clusters; most of the existing sorting and filtering devices for tumor cell clusters adopt a vibration method to avoid blockage of the filter plate. However, the water flow generated by vibration can only disperse the deposits accumulated on the surface of the filter plate, and cannot easily wash away the debris stuck in the upper port of the filter hole, especially the debris stuck inside the filter hole; for this reason, the Chinese patent authorization publication number: CN114797215B, discloses a sorting and filtering device and method for tumor cell clusters, which can form a negative pressure in the area below the filter plate in the filter chamber and can form a positive pressure in the area above the filter plate in the filter chamber, thereby accelerating the filtering speed of tumor cell clusters in the mixed liquid above the filter plate. However, the existing sorting and filtering devices cannot fully sort out tumor cell clusters from the tissue dispersion liquid, and the tumor cell clusters are easily wrapped between adipose tissue, membrane tissue, and connective tissue; in addition, when it is necessary to fully extract tumor cell clusters, the already extracted tumor cell clusters will be continuously impacted, resulting in damage to the tumor cell clusters. In addition, it will also increase the sorting and filtering time. Summary of the Invention

[0003] To solve the above problems, the present invention proposes a sorting and filtering device for tumor cell clusters used in oncology, which can fully extract tumor cell clusters from the dispersion liquid, ensure the dispersion and filtering efficiency of tumor cell clusters, and can protect the already extracted tumor cell clusters.

[0004] The sorting and filtering device for tumor cell clusters used in oncology of the present invention includes: A bottom pipe seat, both ends of the bottom pipe seat are hollow, a partition is integrally formed in the middle of the bottom pipe seat, and a grid groove is opened above the partition of the bottom pipe seat; a pipe passing hole and an exhaust hole are opened in the middle of the partition; A flow storage pipe column; the bottom of the flow storage pipe column is screwed to the top surface of the bottom pipe seat; a convex-shaped diversion hole is provided at the bottom of the flow storage pipe column; a top cover is fixed to the top of the flow storage pipe column; a liquid inlet valve is provided in the middle of the top cover; a plurality of flow channels are opened on the inner wall of the flow storage pipe column; Filter unit, the filter unit includes an upper filter and a lower filter which are interconnected and have the same model; a pneumatic valve is connected to the bottoms of the upper filter and the lower filter; drain ports are provided at the bottoms of the upper filter and the lower filter; the liquid inlet end of the upper filter is communicated with a liquid inlet valve; the pneumatic valve of the lower filter is connected with a collecting pipe; the collecting pipe hermetically passes through the flow accumulation pipe column and movably passes through the pipe passing hole; Scrubbing unit, the scrubbing unit includes a Venturi injector movably passing through the grid slot; the liquid inlet end of the Venturi injector is hermetically fitted with the diversion hole; the output end of the Venturi injector is connected with a riser pipe, and the upper end of the riser pipe is connected to the flow retardation hopper; a socket is connected to the bottom of the flow retardation hopper; Interception filter unit, the bottom of the interception filter unit is inserted into the liquid inlet valve, and the socket is movably inserted into the top of the interception filter unit; Pneumatic unit, the pneumatic unit is respectively connected to the input end of the Venturi injector and the pneumatic valve; Collection tank, the collection tank is movably fitted inside the bottom pipe seat and is movably inserted into the collecting pipe.

[0005] During operation, the fresh tissue dispersion liquid is sent into the interception filter unit, and the larger tissues such as adipose tissue, membrane tissue, and connective tissue in the tissue dispersion liquid are intercepted by the interception filter unit; then, the dispersion liquid and the tumor cell mass are injected into the filter unit. At this time, the pneumatic valve at the bottom of the lower filter is locked, and the dispersion liquid and the tumor cell mass are collected through the lower filter. When the scrubbing unit reaches the action time, the pneumatic valve at the bottom of the upper filter is locked; at this time, the pneumatic unit provides a pressure air source to the Venturi injector, the dispersion liquid in the flow accumulation pipe column enters the Venturi injector through the diversion hole, and the dispersion liquid is formed into a jet through the air source and sent into the riser pipe, and is buffered by the flow retardation hopper; after being stored by the flow retardation hopper, it is sent back into the interception filter unit again, and the larger tissues such as adipose tissue, membrane tissue, and connective tissue intercepted by the interception filter unit are washed; then, the pneumatic valve at the bottom of the lower filter is opened, the tumor cell mass and the remaining dispersion liquid are sent into the collection tank for collection, and the remaining dispersion liquid is discharged; to avoid the enriched tumor cell mass being washed away; then, before the next action of the Venturi injector, the pneumatic valve at the bottom of the upper filter is opened, and the tumor cell mass and the dispersion liquid flow into the lower filter, realizing the flushing of the filter membrane of the lower filter. At the same time, the lower filter is used to filter the dispersion liquid again; after the upper filter is emptied, the pneumatic valve at the bottom of the upper filter is locked again, and the Venturi injector is opened to act, and this is carried out multiple times in sequence to realize the re - collection of the tumor cell mass.

[0006] Further, a pneumatic pipe is radially and hermetically inserted into the flow accumulation pipe column; one end of the pneumatic pipe is rotatably fixed to the pneumatic valve, and the other end is connected to the pneumatic unit; by providing a pressure air source to the pneumatic pipe through the pneumatic unit, the pressure air source is injected into the pneumatic valve to realize the control of the action of the pneumatic valve, and the pneumatic pipe can support the filter unit.

[0007] Further, the air pressure unit includes a flow dividing block. A transverse flow channel is provided inside the flow dividing block, and three longitudinal flow channels penetrate through the transverse flow channel. One end of the transverse flow channel is connected to a pressure gas source through a pressure regulating valve, and the other end is connected to a Venturi injector through a first switching valve and a hose. An exhaust valve is screwed on one of the longitudinal flow channels, and second switching valves are screwed on the other two longitudinal flow channels. The second switching valves are communicated with an air pressure valve through transition hoses. When passing through, the clean gas source enters the transverse flow channel through the pressure regulating valve, and the pressure regulating valve is adjusted according to the pressure requirement for pressure response. The gas source with the pressure adjusted is sent to the first switching valve or the second switching valve. Thus, a pressure air flow is provided for the Venturi injector through the first switching valve to form a jet of the dispersion liquid and inject it into the slow flow hopper. Or pressure is provided for the air pressure valve through the second switching valve to lock the second switching valve. When the second switching valve needs to be opened again, the pressure regulating valve is turned off and the exhaust valve is opened to discharge the gas source inside the pressure regulating valve to the outside.

[0008] Further, the bottom socket is movably inserted into the socket of the shaker. Through the oscillating force of the shaker and in cooperation with the backflow scouring of the jet, the tumor cell clusters can be fully sorted and filtered, and the filtering efficiency can be improved.

[0009] Further, a plurality of Y-shaped support blocks are movably inserted into the top surface of the partition plate. After the bottom of the support block abuts against the top surface of the collection tank, the upper part of the support block is clamped with the Venturi injector. During use, first, the Venturi injector is embedded into the grid slot and the Venturi injector is aligned with the diversion hole. Then, the collection tank is embedded inside the bottom socket, and the support block is pushed up by the collection tank to clamp and limit the support block with the Venturi injector.

[0010] Further, the collection tank includes an outer cup body. A collection filter tank is embedded on the top surface of the outer cup body. A vent hole and a through hole for inserting a collection pipe are provided on the top surface of the collection filter tank. A flow storage gap is provided between the collection filter tank and the outer cup body. The intercepting and filtering unit includes a first inner filter tank. A first flow collecting tank is provided outside the first inner filter tank. A first docking plug is provided on the first flow collecting tank. The first docking plug is docked with the first inner filter tank. The upper filter and the lower filter include a second inner filter tank. A second flow collecting tank is provided outside the second inner filter tank. A liquid discharge port is opened at the bottom of the second flow collecting tank. The bottom of the second flow collecting tank is communicated with the air pressure valve. A second docking plug is provided at the top of the second flow collecting tank. The first flow collecting tank and the second flow collecting tank include a tank body, and a cover body is screwed on the top of the tank body.

[0011] The interception and filtration unit receives fresh tissue dispersion liquid. The fresh tissue dispersion liquid enters the interception and filtration unit. The adipose tissue, membrane tissue, and connective tissue are intercepted by the filter membrane of the first inner filter tank, so that the dispersion liquid and tumor cell clusters enter the first flow collection tank. The dispersion liquid and tumor cell clusters are sent into the second inner filter tank through the first flow collection tank. The dispersion liquid is separated into the second flow collection tank through the second inner filter tank, and the dispersion liquid is sent into the flow channel through the drain port of the second flow collection tank, so that the dispersion liquid flows to the inner bottom of the flow storage pipe column. After the treatment is completed, the tumor cell clusters and residual dispersion liquid in the second inner filter tank are sent into the collection filter tank. The residual dispersion liquid is discharged into the flow storage gap through the collection filter tank. The sorted and filtered tumor cell clusters can be enriched through the collection filter tank.

[0012] Further, the collection filter tank, the first inner filter tank, and the second inner filter tank all include an outer filter cylinder and an inner filter cylinder, and a filter membrane is clamped between the outer filter cylinder and the inner filter cylinder; the pore size of the filter membrane of the collection filter tank and the second inner filter tank is 8-15 μm; the pore size of the filter membrane of the first inner filter tank is 50-80 μm; during operation, when the dispersion liquid is sent into the first inner filter tank, the adipose tissue, membrane tissue, and connective tissue are intercepted by the filter membrane of the first inner filter tank, so that the dispersion liquid and tumor cell clusters enter the second inner filter tank; the dispersion liquid is discharged outside the second inner filter tank through the second inner filter tank, and finally the collected tumor cell clusters are sent into the collection filter tank for collection, and then the dispersion liquid is discharged from the collection filter tank and collected into the flow storage gap; finally, the enriched tumor cell clusters can be obtained by directly taking out the collection filter tank.

[0013] Further, the pneumatic valve includes a valve tube with a three-way structure. A valve core cavity is arranged in the middle of the valve tube. The upper end and the lower end of the valve tube are liquid inlets and outlets; a valve core is movably arranged at the middle end of the valve tube. The valve core and the valve core cavity are movably fitted. A first spring body is movably fitted inside the valve core. A fixed core column is fixed inside the valve core cavity. The fixed core column is embedded in the valve core cavity and abuts against the first spring body; a pneumatic tube is screwed to the middle end of the valve tube; the end of the pneumatic tube abuts against the valve core; when the pneumatic valve acts, the gas source is injected into the middle end of the valve tube through the pneumatic tube. The gas source pushes the valve core into the valve core cavity. The valve core slides along the fixed core column, and the first spring body is compressed, realizing the blocking of the liquid inlet and outlet of the valve tube; when it is necessary to unlock the valve tube, the gas source in the pneumatic tube is discharged. At this time, under the elastic force of the first spring body, the valve core is reset, so that the valve core is separated from the valve core cavity.

[0014] Furthermore, a plurality of baffle plates are arranged inside the flow retardation hopper; the socket joint includes a hose section communicated with the bottom of the flow retardation hopper, a plug is connected to the bottom of the hose section; a second spring body is arranged between the plug and the flow retardation hopper in the hose section; through the large cavity of the flow retardation hopper and the obstruction of the baffle plates, the flow velocity of the jet can be reduced; when the socket joint is inserted into the intercepting and filtering unit, the plug is inserted into the top of the intercepting and filtering unit, and when the socket joint needs to be pulled out, the plug is lifted upwards to compress the second spring body, so that the socket joint is separated from the intercepting and filtering unit.

[0015] Furthermore, a liquid level sensing unit is arranged at the lower part of the Venturi injector. The liquid level sensing unit adopts two electrode heads. When the Venturi injector stores liquid, the two electrode heads are short-circuited, and the liquid level sensing unit can detect the liquid level; the liquid level sensing unit triggers the air pressure unit through the controller of the whole machine control; the Venturi injector acts, and the filtrate is formed into a jet and is pumped back to the intercepting and filtering unit; the continuous working state of the air pressure unit is avoided through the liquid level sensing unit.

[0016] Compared with the prior art, the sorting and filtering device for tumor cell clusters in the oncology department of the present invention uses the jet provided by the brushing unit, can fully wash the adipose tissue, membrane tissue and connective tissue in the dispersion liquid, realizes multiple brushings of the components of the dispersion liquid, can fully extract the tumor cell clusters from the dispersion liquid, and through the cooperation of the upper filter and the lower filter, can separately collect the tumor cell clusters obtained by completing the dispersion filtration, avoid being washed by the filtrate twice, can protect the tumor cell clusters, and can avoid the enrichment of the tumor cell clusters, affecting the dispersion filtration effect of the filter membrane. Description of the Drawings

[0017] Figure 1 It is a schematic diagram of the overall structure of the sorting and filtering device for tumor cell clusters of the present invention.

[0018] Figure 2 For the present invention Figure 1 The partial enlarged structural schematic diagram at position A in it.

[0019] Figure 3 It is a schematic diagram of the internal structure of the axial section of the bottom pipe seat and the liquid storage pipe column of the present invention.

[0020] Figure 4 It is a schematic diagram of the overall structure of the collection tank of the present invention.

[0021] Figure 5 It is a schematic diagram of the overall structure of the intercepting and filtering unit of the present invention.

[0022] Figure 6 It is a schematic diagram of the overall structure of the upper filter of the present invention.

[0023] Figure 7 It is a schematic diagram of the sectional structure of the air pressure valve of the present invention.

[0024] Figure 8 This is a schematic diagram of the installation structure of the Venturi injector and the liquid level sensing unit of the present invention.

[0025] Reference numerals: 1, bottom pipe seat; 2, partition board; 3, grid slot; 4, exhaust hole; 5, flow accumulation pipe column; 6, diversion hole; 7, top cover; 8, liquid inlet valve; 9, upper filter; 10, lower filter; 11, air pressure valve; 12, liquid discharge port; 13, collection pipe; 14, Venturi injector; 15, riser pipe; 16, flow buffering hopper; 17, socket; 18, interception and filtration unit; 19, collection tank; 20, air pressure pipe; 21, flow splitting block; 22, pressure regulating valve; 23, first switching valve; 24, emptying valve; 25, second switching valve; 26, support block; 27, outer cup body; 28, collection and filtration tank; 29, air leakage hole; 30, first inner filtration tank; 31, first flow collection tank; 32, first docking plug; 33, second inner filtration tank; 34, second flow collection tank; 35, second docking plug; 36, valve pipe; 37, valve core cavity; 38, valve core; 39, first spring body; 40, centering column; 41, hose section; 42, plug; 43, second spring body; 44, liquid level sensing unit. Detailed implementation manners

[0026] Example: As Figures 1 to 8 shown, a sorting and filtering device for tumor cell clusters used in oncology departments includes: A bottom pipe seat 1, both ends of the bottom pipe seat 1 are hollow, a partition board 2 is integrally formed in the middle of the bottom pipe seat 1, and a grid slot 3 is opened above the partition board 2 of the bottom pipe seat 1; a pipe passing hole and an exhaust hole 4 are opened in the middle of the partition board 2; A flow accumulation pipe column 5; the bottom of the flow accumulation pipe column 5 is screwed to the top surface of the bottom pipe seat 1; a convex diversion hole 6 is provided at the bottom of the flow accumulation pipe column 5; a top cover 7 is fixed to the top of the flow accumulation pipe column 5; a liquid inlet valve 8 is provided in the middle of the top cover 7; a plurality of flow channels are opened on the inner wall of the flow accumulation pipe column 5; A filtering unit, the filtering unit includes an upper filter 9 and a lower filter 10 which are interconnected and have the same model; an air pressure valve 11 is connected to the bottoms of the upper filter 9 and the lower filter 10; a liquid discharge port 12 is provided at the bottoms of the upper filter 9 and the lower filter 10; the liquid inlet end of the upper filter 9 is communicated with the liquid inlet valve 8; the air pressure valve 11 of the lower filter 10 is connected with a collection pipe 13; the collection pipe 13 is hermetically passed through the flow accumulation pipe column 5 and movably passed through the pipe passing hole; A brushing unit, the brushing unit includes a Venturi injector 14 movably passing through the grid slot 3; the liquid inlet end of the Venturi injector 14 is hermetically fitted with the diversion hole 6; the output end of the Venturi injector 14 is connected with a riser pipe 15, and the upper end of the riser pipe 15 is connected to a flow buffering hopper 16; the bottom of the flow buffering hopper 16 is connected with a socket 17; The intercepting and filtering unit 18, the bottom of the intercepting and filtering unit 18 is plugged into the liquid inlet valve 8, and the plug joint 17 is movably plugged into the top of the intercepting and filtering unit 18; The air pressure unit, the air pressure unit is respectively connected to the input end of the Venturi injector 14 and the air pressure valve 11; The collection tank 19, the collection tank 19 is movably fitted inside the bottom pipe seat 1 and is movably plugged into the collection pipe 13.

[0027] During operation, the fresh tissue dispersion liquid is sent into the intercepting and filtering unit 18, and the larger tissues such as adipose tissue, membrane tissue, and connective tissue in the tissue dispersion liquid are intercepted by the intercepting and filtering unit 18; then, the dispersion liquid and the tumor cell mass are injected into the filtering unit. At this time, the air pressure valve 11 at the bottom of the lower filter 10 is locked, and the dispersion liquid and the tumor cell mass are collected through the lower filter 10. When the brushing unit reaches the action time, the air pressure valve 11 at the bottom of the upper filter 9 is locked; at this time, the air pressure unit provides a pressure air source to the Venturi injector 14, and the dispersion liquid in the flow accumulation pipe column 5 enters the Venturi injector 14 through the diversion hole 6, and the dispersion liquid is formed into a jet through the air source and sent into the riser pipe 15 and buffered by the flow buffering hopper 16; after being buffered by the flow buffering hopper 16, it is sent back into the intercepting and filtering unit 18 again, and the larger tissues such as adipose tissue, membrane tissue, and connective tissue intercepted by the intercepting and filtering unit 18 are flushed and cleaned; then, the air pressure valve 11 at the bottom of the lower filter 10 is opened, and the tumor cell mass and the remaining dispersion liquid are sent into the collection tank 19 for collection, and the remaining dispersion liquid is discharged; to prevent the enriched tumor cell mass from being washed away; then, before the next action of the Venturi injector 14, the air pressure valve 11 at the bottom of the upper filter 9 is opened, and the tumor cell mass and the dispersion liquid flow into the lower filter 10 to realize the flushing of the filter membrane of the lower filter 10. At the same time, the dispersion liquid is filtered again by the lower filter 10; after the upper filter 9 is emptied, the air pressure valve 11 at the bottom of the upper filter 9 is locked again, and the Venturi injector 14 is opened for action, and this is carried out multiple times in sequence to realize the re-collection of the tumor cell mass.

[0028] The flow accumulation pipe column 5 is radially and sealingly plugged with an air pressure pipe 20; one end of the air pressure pipe 20 is rotatably fixed to the air pressure valve 11, and the other end is connected to the air pressure unit; a pressure air source is provided to the air pressure pipe 20 through the air pressure unit, and the pressure air source is injected into the air pressure valve 11 to realize the control of the action of the air pressure valve 11, and the air pressure pipe 20 can support the filtering unit.

[0029] The air pressure unit includes a flow divider block 21, a transverse flow channel is provided inside the flow divider block 21, and three longitudinal flow channels are passed through the transverse flow channel; one end of the transverse flow channel is connected to the pressure gas source through a pressure regulating valve 22, and the other end is connected to the venturi ejector 14 through a first switch valve 23 and a hose; an exhaust valve 24 is screwed on one of the longitudinal flow channels, and the other two longitudinal flow channels are screwed on the second switch valve 25; the second switch valve 25 is connected to the air pressure valve 11 through a transition hose; when passing through, the clean air source enters the transverse flow channel through the pressure regulating valve 22 The pressure regulating valve 22 is adjusted to respond to the pressure according to the pressure requirement, and the gas source with pressure adjustment is sent to the first switch valve 23 or the second switch valve 25; thereby, the first switch valve 23 provides a pressurized airflow for the venturi ejector 14, so that the dispersion is formed into a jet and injected into the slow flow bucket 16; or the second switch valve 25 provides pressure for the air pressure valve 11, so that the second switch valve 25 is locked; when the second switch valve 25 needs to be opened again, the pressure regulating valve 22 is closed, and the drain valve 24 is opened to discharge the gas source inside the pressure regulating valve 22.

[0030] The bottom tube seat 1 is movably connected to the plug interface of the oscillator; through the oscillation force of the oscillator and the backflow flushing of the jet, the tumor cell clusters can be fully sorted and filtered, and the filtering efficiency can be improved.

[0031] A plurality of Y-shaped support blocks 26 are movably inserted on the top surface of the partition 2. After the bottom of the support block 26 abuts against the top surface of the collecting tank 19, the upper part of the support block 26 is engaged with the Venturi ejector 14. When in use, the Venturi ejector 14 is first embedded in the grille slot 3, and the Venturi ejector 14 is aligned with the guide hole 6. Then, the collecting tank 19 is embedded in the inner side of the bottom pipe seat 1, and the support block 26 is pushed up by the collecting tank 19 to achieve the engagement and limiting of the support block 26 and the Venturi ejector 14.

[0032] The collecting tank 19 comprises an outer cup body 27, a collecting filter tank 28 is embedded on the top surface of the outer cup body 27, and a vent hole 29 and a through hole plugged into the collecting pipe 13 are arranged on the top surface of the collecting filter tank 28; a flow storage gap is arranged between the collecting filter tank 28 and the outer cup body 27; the intercepting filter unit 18 comprises a first inner filter tank 30, a first collecting tank 31 is arranged outside the first inner filter tank 30; a first docking plug 32 is arranged on the first collecting tank 31; the first docking plug 32 is docked with the first inner filter tank 30; the upper filter 9 and the lower filter 10 comprise a second inner filter tank 33, a second collecting tank 34 is arranged outside the second inner filter tank 33; a drain port 12 is opened at the bottom of the second collecting tank 34; the bottom of the second collecting tank 34 is connected to the air pressure valve 11; a second docking plug 35 is arranged on the top of the second collecting tank 34; the first collecting tank 31 and the second collecting tank 34 comprise a tank body, and a cover body is screwed on the top of the tank body.

[0033] The intercepting and filtering unit 18 receives the fresh tissue dispersion liquid. The fresh tissue dispersion liquid enters the intercepting and filtering unit 18. The fat tissue, membrane tissue, and connective tissue are intercepted by the filter membrane of the first inner filter tank 30, enabling the dispersion liquid and tumor cell clusters to enter the first collecting tank 31. The dispersion liquid and tumor cell clusters are sent to the second inner filter tank 33 through the first collecting tank 31. The dispersion liquid is separated into the second collecting tank 34 through the second inner filter tank 33, and the dispersion liquid is sent into the flow channel through the drain port 12 of the second collecting tank 34, causing the dispersion liquid to flow to the inner bottom of the flow accumulation pipe column 5. After the treatment is completed, the tumor cell clusters and residual dispersion liquid in the second inner filter tank 33 are sent to the collecting filter tank 28, and the residual dispersion liquid is discharged into the flow accumulation gap through the collecting filter tank 28. The sorted and filtered tumor cell clusters can be enriched by taking out the collecting filter tank 28.

[0034] The collecting filter tank 28, the first inner filter tank 30, and the second inner filter tank 33 all include an outer filter cylinder and an inner filter cylinder, and a filter membrane is clamped between the outer filter cylinder and the inner filter cylinder. The filter membrane pore size of the collecting filter tank 28 and the second inner filter tank 33 is 8 - 15 μm. The filter membrane pore size of the first inner filter tank 30 is 50 - 80 μm. During operation, when the dispersion liquid is sent into the first inner filter tank 30, the fat tissue, membrane tissue, and connective tissue are intercepted by the filter membrane of the first inner filter tank 30, enabling the dispersion liquid and tumor cell clusters to enter the second inner filter tank 33. The dispersion liquid is discharged outside the second inner filter tank 33 through the second inner filter tank 33. Finally, the collected tumor cell clusters are sent to the collecting filter tank 28 for collection, and then the dispersion liquid is discharged from the collecting filter tank 28 and collected into the flow accumulation gap. Finally, the enriched tumor cell clusters can be obtained by directly taking out the collecting filter tank 28.

[0035] The pneumatic valve 11 includes a valve pipe 36 with a tee structure. A valve core cavity 37 is provided in the middle of the valve pipe 36. The upper end and the lower end of the valve pipe 36 are liquid inlets and outlets. A valve core 38 is movably arranged at the middle end of the valve pipe 36. The valve core 38 and the valve core cavity 37 are movably fitted. A first spring body 39 is movably fitted inside the valve core 38. A fixing core column 40 is fixed inside the valve core cavity 37. The fixing core column 40 is embedded in the valve core cavity 37 and abuts against the first spring body 39. A pneumatic pipe 20 is screwed to the middle end of the valve pipe 36. The end of the pneumatic pipe 20 abuts against the valve core 38. When the pneumatic valve operates, the air source is injected into the middle end of the valve pipe 36 through the pneumatic pipe 20. The air source pushes the valve core 38 into the valve core cavity 37. The valve core 38 slides along the fixing core column 40, and the first spring body 39 is compressed, realizing the blocking of the liquid inlet and outlet of the valve pipe 36. When it is necessary to unlock the valve pipe 36, the air source in the pneumatic pipe 20 is discharged. At this time, under the elastic force of the first spring body 39, the valve core 38 is reset, causing the valve core 38 to disengage from the valve core cavity 37.

[0036] A plurality of baffle plates are arranged inside the slow flow hopper 16; the socket 17 includes a hose section 41 communicated with the bottom of the slow flow hopper 16, and a plug 42 is connected to the bottom of the hose section 41; a second spring body 43 is arranged between the plug 42 and the slow flow hopper 16 in the hose section 41; through the large cavity of the slow flow hopper 16 and the blockage of the baffle plates, the flow rate of the jet can be reduced; when the socket 17 is inserted into the interception and filtration unit 18, the plug 42 is inserted into the top of the interception and filtration unit 18, and when it is necessary to pull out the socket 17, the plug 42 is lifted to compress the second spring body 43, so that the socket 17 is separated from the interception and filtration unit 18.

[0037] A liquid level sensing unit 44 is arranged at the lower part of the Venturi injector 14. The liquid level sensing unit 44 adopts two electrode heads. When the Venturi injector 14 stores liquid, the two electrode heads are short-circuited, and the liquid level sensing unit 44 can detect the liquid level; the liquid level sensing unit 44 triggers the air pressure unit through the controller of the whole machine control; the Venturi injector 14 acts, and the filtrate is formed into a jet and is pumped back to the interception and filtration unit 18; the continuous working state of the air pressure unit is avoided through the liquid level sensing unit 44.

[0038] The above embodiments are only the preferred embodiments of the present invention. Therefore, all equivalent changes or modifications made according to the structures, features and principles described in the scope of the present invention application are included in the scope of the present invention application.

Claims

1. A tumor cell cluster separation and filtration device for oncology, characterized in that: include: A bottom tube seat, wherein both ends of the bottom tube seat are hollow, a partition is integrally formed in the middle of the bottom tube seat, and a grille slot is provided above the partition; The middle part of the partition is provided with a pipe hole and an exhaust hole; A flow storage column; the bottom of the flow storage column is screwed to the top surface of the bottom tube seat; a convex flow guide hole is arranged at the bottom of the flow storage column; a top cover is fixed on the top of the flow storage column; a liquid inlet valve is arranged in the middle of the top cover; a plurality of flow channels are opened on the inner wall of the flow storage column; A filter unit, the filter unit comprises an upper filter and a lower filter which are interconnected and of the same model; the upper filter and the lower filter are connected to an air pressure valve at the bottom; the upper filter and the lower filter are provided with a liquid discharge port at the bottom; the liquid inlet end of the upper filter is connected to the liquid inlet valve; the air pressure valve of the lower filter is connected to a collecting pipe; the collecting pipe is sealed and passes through the flow storage column and movably passes through the pipe hole; The scrubbing unit comprises a venturi ejector that moves through the grille slot; the liquid inlet end of the venturi ejector is sealed and embedded with the guide hole; the output end of the venturi ejector is connected to a vertical pipe, the upper end of the vertical pipe is connected to the slow flow bucket; the bottom of the slow flow bucket is connected to a plug connector; A retaining filter unit, wherein the bottom of the retaining filter unit is plugged into the liquid inlet valve, and the plug connector is movably plugged into the top of the retaining filter unit; An air pressure unit, the air pressure unit is connected to the input end of the venturi ejector and the air pressure valve respectively; The collecting tank is movably embedded in the inner side of the bottom pipe seat and movably plugged with the collecting pipe.

2. The tumor cell cluster separation and filtration device for oncology according to claim 1, characterized in that: The flow storage column is radially sealed and plugged with an air pressure tube; one end of the air pressure tube is screwed and fixed to the air pressure valve, and the other end is connected to the air pressure unit.

3. The tumor cell cluster separation and filtration device for oncology according to claim 1, characterized in that: The air pressure unit includes a diverter block, a transverse flow channel is opened on the inner side of the diverter block, and three longitudinal flow channels are passed through the transverse flow channel; one end of the transverse flow channel is connected to the pressure gas source through a pressure regulating valve, and the other end is connected to the Venturi ejector through a first switch valve and a hose; an exhaust valve is screwed on one of the longitudinal flow channels, and the other two longitudinal flow channels are screwed on second switch valves; the second switch valve is connected to the air pressure valve through a transition hose.

4. The tumor cell cluster separation and filtration device for oncology according to claim 1, characterized in that: The bottom tube seat is movably plugged into the plug interface of the oscillator.

5. The tumor cell cluster separation and filtration device for oncology according to claim 1, characterized in that: A plurality of Y-shaped support blocks are movably inserted on the top surface of the partition. After the bottom of the support block abuts against the top surface of the collection tank, the upper part of the support block is clamped with the Venturi ejector.

6. The tumor cell cluster separation and filtration device for oncology according to claim 1, characterized in that: The collecting tank comprises an outer cup body, a collecting filter tank is embedded in the top surface of the outer cup body, an air leakage hole and a through hole connected with the collecting pipe are arranged on the top surface of the collecting filter tank; a flow storage gap is arranged between the collecting filter tank and the outer cup body; the intercepting filter unit comprises a first inner filter tank, a first collecting tank is arranged outside the first inner filter tank; a first docking plug is arranged on the first collecting tank; the first docking plug is docked with the first inner filter tank; the upper filter and the lower filter comprise a second inner filter tank, a second collecting tank is arranged outside the second inner filter tank; a drain port is arranged at the bottom of the second collecting tank; the bottom of the second collecting tank is connected to the air pressure valve; a second docking plug is arranged on the top of the second collecting tank; the first collecting tank and the second collecting tank comprise a tank body, a cover body is screwed on the top of the tank body.

7. The tumor cell cluster separation and filtration device for oncology according to claim 6, characterized in that: The collecting filter tank, the first inner filter tank and the second inner filter tank all include an outer filter cartridge and an inner filter cartridge, and a filter membrane is sandwiched between the outer filter cartridge and the inner filter cartridge; the pore size of the filter membrane of the collecting filter tank and the second inner filter tank is 8~15μm; the pore size of the filter membrane of the first inner filter tank is 50~80μm.

8. The tumor cell cluster separation and filtration device for oncology according to claim 1, characterized in that: The pneumatic valve comprises a valve tube with a three-way structure, a valve core cavity is arranged in the middle of the valve tube, the upper end and the lower end of the valve tube are a liquid inlet and a liquid outlet; a valve core is movably arranged at the middle end of the valve tube, the valve core and the valve core cavity are movably engaged, a first spring body is movably engaged inside the valve core, a core-fixing column is fixed inside the valve core cavity, the core-fixing column is embedded in the valve core cavity and abuts against the first spring body; a pneumatic tube is screwed onto the middle end of the valve tube; the end of the pneumatic tube abuts against the valve core.

9. The tumor cell cluster separation and filtration device for oncology according to claim 1, characterized in that: A plurality of baffles are arranged inside the slow-flow bucket; the plug connector comprises a hose section connected to the bottom of the slow-flow bucket, and a plug is connected to the bottom of the hose section; a second spring body is arranged between the plug and the slow-flow bucket of the hose section.

10. The tumor cell cluster separation and filtration device for oncology according to claim 1, characterized in that: A liquid level sensing unit is arranged at the lower part of the venturi ejector.

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