Method for hemoglobin electrophoresis detection

Abstract

The invention discloses a method for hemoglobin electrophoresis detection. The method comprises the following steps: step 1, assembling a sample holder and adding a hemolytic agent into a dilution cup; 2, flushing the structure through a liquid path system and a gas path system; step 3, filling a buffer solution; 4, sampling by a sampling mechanism and dripping into a dilution cup; 5, the sampling mechanism is subjected to self-cleaning; sixthly, repeating the fourth step and the fifth step to complete sampling of all the blood sample test tubes; 7, extracting the blood sample in the dilution cup by using the capillary tube; 8, performing electrophoresis by an electrophoresis module; step 9, discharging waste liquid; tenthly, all the structures are cleaned through the liquid path system and the gas path system; the device has the advantages that the gas path system generates positive pressure and negative pressure so as to drive liquid in the liquid path system to move, and relevant work such as cleaning is achieved; the sampling assembly further samples the blood sample, and the sampling assembly can realize self-cleaning through the cooperation of the liquid path system, the gas path system and the plunger pump.

Description

Technical Field

[0001] The present invention relates to the technical field of medical devices, and particularly to a method for hemoglobin electrophoresis detection. Background Art

[0002] In laboratories or clinics, capillary electrophoresis is usually used to detect hemoglobin in blood samples. In capillary electrophoresis, ions aggregated on the inner wall of the capillary channel move by applying a voltage, thereby generating an electroosmotic flow, which enables various proteins in the blood sample to move for electrophoretic separation, and finally the contents of various proteins are obtained.

[0003] When the electrophoresis module performs electrophoresis operations, after the blood sample is further sampled and enters the capillary, after electrophoresis is completed and the waste liquid is discharged, it is necessary to promptly clean all structures that have come into contact with the blood sample and discharge the waste liquid to avoid cross-mixing of blood samples and affecting subsequent test results. Summary of the Invention

[0004] The technical problem to be solved by the present invention is that after electrophoresis is completed, all structures that have come into contact with the blood sample cannot be efficiently cleaned synchronously, which will affect the continuous progress of the detection work and the detection results. A method for hemoglobin electrophoresis detection is proposed for the above problem, including the following steps: Step 1: Preparation work: Assemble the specimen rack, dilution cup, and blood sample tube and place them below the sampling mechanism, and add hemolytic agent into the cavity of the dilution cup; Step 2: Start the gas path system and the liquid path system, and rinse the electrophoresis module, cathode tank, anode cup, and sampling component with distilled water in the distilled water bottle of the liquid path system, and discharge the waste liquid into the waste liquid bottle of the liquid path system; Step 3: The buffer solution in the buffer solution bottle of the liquid path system enters the cathode tank and the anode cup, and gradually enters the capillary of the electrophoresis module connected to the cathode tank; Step 4: Sampling work: Start the sampling mechanism, the sampling needle extends out of the sampling component, sample the blood sample tube through the plunger pump, and inject the blood sample into the corresponding cavity of the dilution cup; Step 5: The sampling needle returns to the inside of the sampling component, rinse the inner wall of the sampling needle with distilled water in the distilled water bottle, rinse the outer wall of the sampling needle, and discharge the waste liquid into the waste liquid bottle; Step 6: Repeat Step 4 and Step 5 to sequentially complete sampling of all blood sample tubes; Step 7: Electrophoresis work: Place the dilution cup after sampling below the electrophoresis module, extract the blood sample through the capillary extending into the corresponding cavity in turn, remove the dilution cup, and insert the capillary into the anode cup; Step 8: Perform electrophoresis separation on the blood sample in the capillary tube through the electrophoresis module to obtain the content of various proteins in the blood sample; Step 9: Finishing work: After the electrophoresis is completed, the waste liquid in the cathode tank, anode cup and capillary tube is sucked into the waste liquid bottle; Step 10: Clean the capillary with the cleaning liquid in the cleaning liquid bottle of the liquid system and drain the waste liquid into the waste liquid bottle, and then rinse the cathode tank, anode cup and capillary as a whole with distilled water in the distilled water bottle and drain the waste liquid into the waste liquid bottle.

[0005] The technical solution of this embodiment, by setting the structure of the gas circuit system and the liquid circuit system, the gas circuit system generates positive pressure and negative pressure, thereby driving the movement of various liquids in the liquid circuit system, and realizing related processes such as cleaning and electrophoresis; by setting the structure of the sampling component, the blood sample can be further sampled, and at the same time, the sampling component can achieve self-cleaning through the cooperation of the liquid circuit system, the gas circuit system and the plunger pump.

[0006] In a preferred embodiment of the technical solution of the present invention, the blood sample tube is arranged in an opening of the sample rack, the dilution cup is clamped on the outside of the sample rack, the number of dilution cup cavities on the same sample rack is consistent with the number of blood sample tubes, the blood sample tube is inserted into the sample rack through the opening, which is convenient for placement and installation, the dilution cup is used for placing further sampled blood samples for subsequent electrophoresis operation, and the hemolytic agent in the dilution cup can prevent blood coagulation.

[0007] Preferably, the air circuit system comprises an air pump, an air circuit unit, an air storage tank, a safety valve, a plurality of negative pressure pipes and a positive pressure pipe. The negative pressure port of the air pump is connected to the air circuit unit through a negative pressure pipe, the air circuit unit is connected to the waste liquid bottle through another negative pressure pipe, the positive pressure port of the air pump is connected to the air circuit unit through a positive pressure pipe, the air circuit unit is respectively connected to the buffer bottle, the cleaning liquid bottle and the distilled water bottle through a positive pressure pipe with a safety valve, the air storage tank for pressure stabilization is connected to the air circuit unit through a positive pressure pipe, the air pump is used to generate negative pressure and positive pressure to drive the liquid circuit system, the air storage tank is used to adjust and maintain the pressure in the pipe belt, the safety valve prevents the air pressure from being too high and the pipeline from collapsing at the interface, and the negative pressure pipe and the positive pressure pipe are used to connect the various parts of the air circuit system.

[0008] Preferably, the liquid circuit system also includes a first switching valve and a second switching valve, the main port of the first switching valve is connected to the plunger pump, the four sub-ports of the first switching valve are respectively connected to the distilled water bottle, the cathode tank, the sampling needle and the closed spare pipeline, the main port of the second switching valve is connected to the cathode tank, the four sub-ports of the second switching valve are respectively connected to the buffer bottle, the cleaning liquid bottle, the distilled water bottle and the waste liquid bottle, and the liquid circuit system is connected to other structures through the first switching valve and the second switching valve, so that the liquid circuit system can perform corresponding work.

[0009] For the optimization of the technical solution of the present invention, the cathode tank is a closed space. The internal cavity of the cathode tank is inclined. A plurality of first solenoid valves communicating with the internal cavity are arranged on the cathode tank. The first solenoid valve located at the uppermost end of the inclined cavity is communicated with the gas path unit through a positive pressure pipe. The second solenoid valve located at the lowermost end of the inclined cavity is communicated with the waste liquid bottle through a pipe. A first high and low liquid level sensor is arranged on the cathode tank and extends into the cavity. Above the cathode tank, an inlet for the capillary bundle head is also arranged. The cathode tank is closed, and liquid enters its internal cavity through positive pressure. The liquid level rises and gradually submerges the end of the capillary. Under the action of positive pressure, the liquid enters the capillary. The inclined cavity facilitates the complete discharge of waste liquid. The first high and low liquid level sensor is used to define the lowest and highest lines of the liquid level in the cathode tank, and the inlet for the bundle head facilitates the capillary to extend into the cathode tank.

[0010] For the optimization of the technical solution of the present invention, the connection ports of the first switching valve and the second switching valve with the cathode tank are both located above the internal cavity, and the liquid drips from top to bottom, so that the liquid in the pipeline will not be polluted during electrophoresis.

[0011] For the optimization of the technical solution of the present invention, the opening of the anode cup faces upward. A plurality of second solenoid valves are arranged above the opening of the anode cup. The pipeline below the second solenoid valve communicated with the waste liquid bottle extends into the bottom of the anode cup. The pipeline below the second solenoid valve communicated with the buffer solution bottle and the distilled water bottle is located above the anode cup. Second high and low liquid level sensors are arranged on both sides of the second solenoid valve and extend into the anode cup. The second solenoid valve communicated with the waste liquid bottle is used to suck the waste liquid and discharge it to the waste liquid bottle. The second high and low liquid level sensors can respectively define the highest and lowest lines of the liquid level, which can prevent waste caused by the liquid flowing out when the liquid level is too high or prevent the electrophoresis operation from being unable to be completed when the liquid level is too low.

[0012] For the optimization of the technical solution of the present invention, the sampling assembly includes a sampling needle, a cleaning valve and a valve seat. The valve seat is provided with a hole along the vertical direction. The cleaning valve is arranged on one side of the valve seat and communicated with the hole of the valve seat. The cleaning valve is communicated with the waste liquid bottle through a pipe. The sampling needle passes through the hole of the valve seat. The upper end of the sampling needle is connected with the branch port of the first switching valve through a pipe with a liquid level sensor. The sampling needle is used to further sample the blood sample in the blood sample test tube and drip it into the dilution cup. The cleaning valve is used to discharge the waste liquid generated during cleaning.

[0013] For the optimization of the technical solution of the present invention, the sampling assembly can move horizontally, and the sampling needle can move vertically. The overall horizontal movement of the sampling assembly can complete the sampling work for all blood sample test tubes. The vertical movement of the sampling needle facilitates entering the blood sample test tube to extract the blood sample and also facilitates returning to the valve seat for cleaning.

[0014] For the optimization of the technical solution of the present invention, the diameter of the opening is slightly larger than the outer diameter of the sampling needle. After the sampling needle is cleaned with distilled water, the waste distilled water enters the opening. Due to the small diameter of the opening, under the action of negative pressure, a water film is formed in the opening to clean the outer wall of the sampling needle and finally discharge it into the waste liquid bottle.

[0015] The beneficial effects of the present invention compared with the prior art are as follows: In the technical solution of the present invention, by setting the structures of the gas circuit system and the liquid circuit system, the gas circuit system generates positive pressure and negative pressure, thereby driving the movement of each liquid in the liquid circuit system to realize related processes such as cleaning and electrophoresis; by setting the structure of the sampling assembly, further sampling of blood samples can be carried out. At the same time, the sampling assembly can realize self-cleaning through the cooperation of the liquid circuit system, the gas circuit system and the plunger pump. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 is a framework logic schematic diagram of the present invention; Figure 2 is a three-dimensional schematic diagram of the sample rack of the present invention; Figure 3 is a logic schematic diagram of the gas circuit unit of the present invention; Wherein: 1 - sample rack, 11 - blood sample test tube, 12 - dilution cup, 2 - gas circuit system, 21 - air pump, 22 - gas circuit unit, 23 - gas storage tank, 24 - safety valve, 25 - negative pressure pipe, 26 - positive pressure pipe, 3 - liquid circuit system, 31 - buffer solution bottle, 32 - cleaning solution bottle, 33 - distilled water bottle, 34 - waste liquid bottle, 35 - first switching valve, 36 - second switching valve, 4 - cathode tank, 41 - first high and low liquid level sensor, 42 - first solenoid valve, 43 - cluster head inlet, 5 - anode cup, 51 - second high and low liquid level sensor, 52 - second solenoid valve, 6 - plunger pump, 7 - sampling assembly, 71 - sampling needle, 72 - cleaning valve, 73 - valve seat. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0017] Next, the technical solutions in the embodiments of the present invention will be described in detail in conjunction with the attached Figures 1-3 to the present invention. Embodiment 1

[0018] As Figures 1-3 shown, the present invention is a method for hemoglobin electrophoresis detection, which realizes the efficient electrophoresis detection of the whole process of blood sample through the cooperation of the sample rack 1, the gas circuit system 2, the liquid circuit system 3, the cathode tank 4, the anode cup 5, the plunger pump 6, the sampling assembly 7 and the electrophoresis module.

[0019] As Figure 2As shown, the sample rack 1 is a prior art. The sample rack 1 is used to place the blood sample test tube 11, and the blood sample test tube 11 is a prior art. After drawing blood from a patient, the blood is dropped into the blood sample test tube 11, and the blood sample test tube 11 is placed into the opening of the sample rack 1 from top to bottom.

[0020] In this embodiment, the outer side of the sample rack 1 protrudes to form a bracket for clamping the dilution cup 12. The dilution cup 12 is clamped on the bracket. The dilution cup 12 is used to hold and dilute part of the blood sample after further sampling the blood sample in the blood sample test tube 11, so as to complete the electrophoresis process subsequently.

[0021] Furthermore, the dilution cup 12 is concave to form a plurality of cavities for holding blood samples. The number of cavities in the dilution cup 12 is the same as the number of blood sample test tubes 11 on the sample rack 1. After further sampling the blood sample test tube 11, the blood is dropped into the corresponding cavity of the dilution cup to avoid confusion and affecting the test results.

[0022] As Figure 1 shown, the gas path system 2 includes an air pump 21, a gas path unit 22, a gas storage tank 23, a safety valve 24, a plurality of negative pressure tubes 25, and a positive pressure tube 26.

[0023] In this embodiment, the gas path unit 22 is composed of five solenoid valves, namely solenoid valve A, solenoid valve B, solenoid valve C, solenoid valve D, and solenoid valve E. Through the gas path unit 22, the air pressure inside the pipeline of the gas path system 2 can be ensured to be maintained at the required standard.

[0024] As Figure 3 shown, the connection logic of each solenoid valve in the gas path unit 22 is as follows: The three ports of solenoid valve A are respectively connected to port ①, port ⑨, and port ⑩. One end of solenoid valve B is communicated with port ②, another port is directly communicated with the atmosphere, and the last port is respectively connected to port ⑧, port ⑥, and one port of solenoid valve C through a four-way valve. The other two ports of solenoid valve C are respectively connected to port ③ and port ④. The two ports of solenoid valve D are respectively connected to port ⑦ and port ⑤. The last port of solenoid valve D is directly communicated with the atmosphere. One port of solenoid valve E is also communicated with port ⑥ through a three-way valve. Another port of solenoid valve E is communicated with port ⑤ through a three-way valve. The last port of solenoid valve E is directly communicated with the atmosphere.

[0025] In this embodiment, port ⑦ is connected to a negative pressure sensor, and port ⑧ is connected to a positive pressure sensor. Both the negative pressure sensor and the positive pressure sensor are prior arts. Through the negative pressure sensor and the positive pressure sensor, combined with the control system, the upper and lower limits of the air pressure in the positive pressure tube 26 can be maintained, so that the air pressure in the positive pressure tube 26 is always maintained within the required air pressure range.

[0026] In this embodiment, after an air filter is installed at port ⑨, it is connected to the atmosphere. The air filters are all prior arts, and the air filters can prevent dust and impurities in the atmosphere from entering the interior.

[0027] Port ④ in this embodiment is also directly connected to the air for discharging overflow gas. It has the same function as the ports connected to the atmosphere above, and is used to exhaust and adjust the air pressure in the positive pressure pipe 26, so that the air pressure in the positive pressure pipe 26 is always maintained within the required standard range.

[0028] In this embodiment, the air pump 21 is a prior art and can generate negative pressure and positive pressure.

[0029] In this embodiment, the negative pressure port of the air pump 21 is connected to port ① of the gas path unit 22 through the negative pressure pipe 25, and port ⑩ is connected to the waste liquid bottle 34 through the negative pressure pipe 25. It should be noted that the negative pressure pipe 25 connected to the waste liquid bottle 34 extends into the waste liquid bottle 34 and is above its internal space, ensuring that the end of the negative pressure pipe 25 can always be above the liquid level in the bottle body.

[0030] In this embodiment, the positive pressure port of the air pump 21 is connected to port ③ of the gas path unit 22 through the positive pressure pipe 26. Port ② branches through a positive pressure pipe 26 and is respectively connected to the buffer solution bottle 31, the cleaning solution bottle 32, and the distilled water bottle 33. Specifically, multiple branch pipes can be connected to the positive pressure pipe 26 through a three-way valve, and the branch pipes extend into the buffer solution bottle 31, the cleaning solution bottle 32, and the distilled water bottle 33 to achieve the purpose of connecting with them. It should be noted that the branch pipes also only extend above their interiors and always remain above the liquid level in the bottle body.

[0031] Furthermore, a safety valve 24 is installed on the main pipe part of the positive pressure pipe 26. The safety valve 24 is used to maintain the pressure in the pipeline below the limit value. After the pressure exceeds the limit value, the safety valve 24 automatically opens to adjust the pressure. After the pressure is lower than the limit value, the safety valve 24 automatically closes. The safety valve 24 can prevent the pressure from being too high and causing the pipeline to burst at the interface.

[0032] The air storage tank 23 in this embodiment is connected to port ⑥ through the positive pressure pipe 26. The air storage tank 23 is used to maintain the pressure value in the pipeline and keep the pressure value in the pipeline within the required range.

[0033] As Figure 1 shown, the liquid path system 3 includes a flushing solution bottle 31, a cleaning solution bottle 32, a distilled water bottle 33, a waste liquid bottle 34, a first switching valve 35, and a second switching valve 36. The first switching valve 35 and the second switching valve 36 both belong to prior arts. The first switching valve 35 and the second switching valve 36 can only be docked with one sub-port through the main port at the same time, and the sub-ports cannot be docked and connected to each other.

[0034] The main port of the first switching valve 35 in this embodiment is connected to the plunger pump 6 through a pipeline. The four branch ports of the first switching valve 35 are respectively connected to the distilled water bottle 33, the cathode tank 4, the sampling needle 71 and a closed standby pipeline through pipelines. Among them, the pipeline connected to the distilled water bottle 33 extends into its bottom to ensure that it is always below the liquid level. A liquid level sensor is installed on the pipeline connected to the sampling needle 71 to prevent excessive blood samples from being drawn through the sampling needle 71 and entering the first switching valve 35, causing pipeline contamination.

[0035] Furthermore, the standby pipeline is a closed pipeline and is used when adding hemolytic agent. Since the liquid in the bottle body of the liquid path system 3 will change, when the liquid in a certain bottle body is hemolytic agent, then the pipeline connected to this bottle body is disconnected and sealed, and the standby pipeline is connected to this bottle body. Under the positive pressure of the gas path system 2, the hemolytic agent enters the first switching valve 35 through the pipeline and enters the pipeline connected to its main port. Then the first switching valve 35 starts to work, and the branch ports are switched. The branch port connected to the sampling needle 71 enters the working state. Under the positive pressure of the plunger pump 6, the hemolytic agent enters the sampling needle 71 through the pipeline. At this time, the sampling needle 71 can be moved above the dilution cup 12 to realize the automatic addition of hemolytic agent.

[0036] Furthermore, the plunger pump 6 is a prior art. The plunger pump 6 cooperates with the sampling needle 71 to extract the blood sample in the blood sample tube 11, and further sample and then drop it into the dilution cup 12.

[0037] The main port of the second switching valve 36 in this embodiment is communicated with the cathode tank 4. The four branch ports of the second switching valve 36 are respectively connected to the buffer solution bottle 31, the cleaning solution bottle 32, the distilled water bottle 33 and the waste liquid bottle 34.

[0038] Furthermore, the first switching valve 35 and the second switching valve 36 are communicated with the cathode tank 4 through pipelines. Specifically, an opening can be made at the upper end of the cathode tank 4 and connected to the opening through a pipeline to realize the communication between the first switching valve 35 and the second switching valve 36 and the cathode tank 4. And because the pipeline is located above the cathode tank 4, it is not easy for the liquid to flow back into the pipeline, which can avoid the waste liquid after electrophoresis from contaminating the liquid and affecting the subsequent detection results.

[0039] Furthermore, the four branch ports of the second switching valve 36 respectively extend into the bottoms of the buffer solution bottle 31, the cleaning solution bottle 32, the distilled water bottle 33 and the waste liquid bottle 34 through pipelines, and the pipelines need to always remain below the liquid level inside the bottle body, so that the gas path system 2 can always work properly.

[0040] The inner cavity of the cathode tank 4 in this embodiment is empty and is a closed space. The internal cavity of the cathode tank 4 is inclined, and the inclined cavity is convenient for discharging waste liquid.

[0041] In this embodiment, two first solenoid valves 42 are installed on the cathode tank 4. The first solenoid valves 42 are in communication with the interior of the cathode tank 4. One of the first solenoid valves 42 is located at the uppermost end of the inclined cavity. This first solenoid valve 42 is connected to port ⑤ of the gas path unit 22 through a positive pressure pipe 26. The positive pressure generated by the gas path system 2 can promote the discharge of waste liquid and also promote the entry of buffer solution into the capillary of the electrophoresis module.

[0042] Further, the other first solenoid valve 42 is located at the lowermost end of the inclined cavity. This first solenoid valve 42 is connected to the waste liquid bottle 34 through a pipe. It should be noted that this pipe extends into the bottom of the waste liquid bottle 34 and always remains below the liquid level. Therefore, when the gas path system generates negative pressure, the waste liquid in the cathode tank 4 will be suctioned into the waste liquid bottle 34.

[0043] The pipes connecting the above-mentioned first switching valve 35 and second switching valve 36 to the cathode tank 4 can specifically refer to the following method: Open holes in the upper wall of the cathode tank 4 and connect the communication ports through threaded connections, and then connect the pipes to the communication ports, thereby realizing the connection between the first switching valve 35 and the second switching valve 36 and the cathode tank 4.

[0044] In this embodiment, a first high and low liquid level sensor 41 is provided on the cathode tank 4. The first high and low liquid level sensor 41 is two liquid level sensors, both of which belong to the prior art and are respectively used to define the highest line and the lowest line of the liquid level. It can prevent the liquid level from being too high and entering the first switching valve 35 and the second switching valve 36 through the pipe, causing pollution and affecting the detection results. At the same time, it can also prevent the liquid level from being too low and unable to perform electrophoresis operations.

[0045] Further, the first high and low liquid level sensors 41 both extend into the interior of the cathode tank 4.

[0046] In this embodiment, a cluster head inlet 43 for the capillary is also provided above the cathode tank 4. The electrophoresis module is a prior art. One side of its capillary is attached to each other through a cluster head and then snapped into the cluster head inlet 43.

[0047] Further, the number of capillaries is the same as the number of cavities in the dilution cup 12. During electrophoresis detection, each capillary extends into the corresponding cavity of the dilution cup 12 respectively, and the blood sample enters the capillary to achieve the purpose of loading, so as to facilitate subsequent electrophoresis through the capillary.

[0048] Although the cathode tank 4 in this embodiment is connected to other parts, its internal cavity needs to be kept airtight so that the gas path system 2 can act on it.

[0049] In this embodiment, the opening of the anode cup 5 faces upward. The other end of the capillary of the electrophoresis module is located above the opening of the anode cup 5. At the same time, three second solenoid valves 52 are also provided for the anode cup 5, and all three second solenoid valves 52 are located above the anode cup 5.

[0050] In this embodiment, one of the second solenoid valves 52 is connected to the waste liquid bottle 34 through a pipeline, and this pipeline needs to extend below the liquid level. At the same time, this second solenoid valve 52 extends into the bottom of the inner cavity of the anode cup 5 through a pipeline for extracting waste liquid into the waste liquid bottle 34; the other two second solenoid valves 52 are respectively connected to the buffer solution bottle 31 and the distilled water bottle 33 through pipelines, and these pipelines also need to extend below the liquid level of the bottle body respectively. At the same time, these two second solenoid valves 52 also extend into the inner cavity of the anode cup 5 through pipelines, facilitating the positive pressure generated by the gas path system 2 to press the buffer solution and distilled water into the anode cup 5 through the pipelines.

[0051] Further, the three second solenoid valves 52 can be fixed above the anode cup 5 by connecting to the mounting bracket, and the three second solenoid valves 52 are arranged in a row.

[0052] In this embodiment, the second high and low liquid level sensor 51 is installed outside the second solenoid valve 52, and the second high and low liquid level sensor 51 extends into the anode cup 5. The second high and low liquid level sensor 51 is the same product as the first high and low liquid level sensor 41, and its function is also the same as that of the first high and low liquid level sensor 41.

[0053] It should be noted that the anode cup 5 is not closed. Therefore, when the liquid level is too high, it will cause liquid overflow, resulting in waste and pollution to other parts, increasing the cleaning difficulty.

[0054] In this embodiment, the sampling assembly 7 includes a sampling needle 71, a cleaning valve 72, and a valve seat 73.

[0055] In this embodiment, the valve seat 73 is drilled vertically from top to bottom, and this opening is used for passing the sampling needle 71, and the diameter of this opening is slightly larger than the outer diameter of the sampling needle 71.

[0056] In this embodiment, the cleaning valve 72 is installed on one side of the valve seat 73 and is communicated with the opening of the valve seat 73. At the same time, the cleaning valve 72 is connected to the waste liquid bottle 34 through a pipeline, and this pipeline needs to extend to the bottom end inside the waste liquid bottle 34 and remain below the liquid level.

[0057] In this embodiment, the sampling needle 71 passes through the opening, and the upper end of the sampling needle 71 is connected to the branch port of the first switching valve 35 through a pipeline, and a liquid level sensor is installed on this pipeline to prevent excessive blood extraction and pollution of the first switching valve 35.

[0058] In this embodiment, the lower end of the sampling needle 71 can extend out of the valve seat 73 to further sample the blood sample in the blood sample tube 11.

[0059] Further, when cleaning is required, the sampling needle 71 is displaced upward so that the lower end port thereof is located within the opening of the valve seat 73. Distilled water enters the sampling needle 71 and flows out from its lower port. Due to the negative pressure effect of the cleaning valve 72 and in cooperation with the relatively small-diameter opening, the distilled water will be sucked through the cleaning valve 72 and discharged into the waste liquid bottle 34 through the pipeline.

[0060] The distilled water flows out from the inside of the sampling needle 71 to complete the cleaning of the inner wall, and then flows out from the lower port. Under the negative pressure suction of the cleaning valve 72, a water film is formed inside the opening. And due to the negative pressure effect, the water in the water film continuously tumbles up and down, thereby realizing the cleaning of the outer wall of the sampling needle 71.

[0061] In this embodiment, there are multiple pipelines connected to the flushing liquid bottle 31, the cleaning liquid bottle 32, the distilled water bottle 33 and the waste liquid bottle 34. Except for the negative pressure pipe 25 and the positive pressure pipe 26 of the gas path system 2, the rest need to extend below the liquid level, and these pipelines can be combined and extend to the bottom of the bottle body through a main pipe and be located below the liquid level.

[0062] In this embodiment, the position of the anode cup 5 needs to be adjustable. Initially, it is located at the lower end of the capillary port. Then, when the capillary needs to extract the blood sample in the dilution cup 12, the anode cup 5 needs to descend a certain distance and then move away from the capillary. At the same time, the dilution cup 12 moves to directly below the capillary, and the dilution cup 12 gradually rises so that the port of the capillary extends into the cavity of the dilution cup 12. When the capillary finishes extraction, the height of the dilution cup 12 drops and it moves away from the capillary. At the same time, the anode cup 5 returns to directly below the capillary and moves upward so that the port of the capillary is immersed in the anode cup 5 to facilitate subsequent electrophoresis work.

[0063] This is achieved by the following example: By a platform that can rotate in the horizontal direction and move in the vertical direction, the above process can be realized. The horizontal and vertical movements of the platform can be controlled by a motor respectively. The platform is fixedly connected to the mounting bracket for installing the second solenoid valve 52, and the electrophoresis module is also connected to this mounting bracket. The cathode tank 4 remains relatively stationary with respect to the electrophoresis module. Both the anode cup 5 and the dilution cup 12 are located on the movable platform, and the two are driven by the platform to move, thereby realizing the relative displacement with respect to the electrophoresis module, that is, the relative displacement with respect to the capillary.

[0064] In this embodiment, the sampling assembly 7 can move horizontally, and the sampling needle 71 can move vertically. For example, it can be achieved in the following way: The valve seat 73 of the sampling assembly 7 is supported by a bracket and is located above the sample rack 1. The bracket can be moved arbitrarily in the horizontal direction through a cross slide, so as to realize the horizontal movement of the sampling assembly 7. The sampling needle 71 is slidably connected to the bracket and is positioned in the vertical direction through a motor.

[0065] A method for hemoglobin electrophoresis detection in this embodiment is as follows: Step 1: Preparation work: Assemble the sample rack 1, the dilution cup 12 and the blood sample test tube 11 and place them below the sampling mechanism 7. Add a hemolytic agent into the cavity of the dilution cup 12. Specifically: Insert the blood sample test tube 11 containing the blood sample into the sample rack 1 in sequence, then take the corresponding dilution cup 12, snap the dilution cup 12 onto the bracket outside the sample rack 1, place the assembled sample rack 1 below the sampling assembly 7, and drop an equal amount of hemolytic agent into all the cavities of the dilution cup 12 in sequence.

[0066] Step 2: Start the gas circuit system 2 and the liquid circuit system 3. Use the distilled water in the distilled water bottle 33 of the liquid circuit system 3 to rinse the electrophoresis module, the cathode tank 4, the anode cup 5 and the sampling assembly 7, and discharge the waste liquid into the waste liquid bottle 34 of the liquid circuit system 3. Specifically: The gas circuit system 2 works. The positive pressure port of the air pump 21 generates positive pressure, and the distilled water is pressed into the first switching valve 35, the second switching valve 36 and the second solenoid valve 52 connected thereto through the positive pressure pipe from the distilled water bottle 33. At the same time, the negative pressure port of the air pump 21 generates negative pressure, so that the waste liquid bottle 34 is in a negative pressure state to realize the suction of the waste liquid. The distilled water entering the first switching valve 35 and the second switching valve 36 from the branch ports will respectively enter the main port pipelines of the first switching valve 35 and the second switching valve 36. Then the first switching valve 35 switches to the working branch port. Under the action of the plunger pump 6, its main port will send the distilled water in the pipeline into the working branch port at this time through the positive pressure generated by the plunger pump 6, that is, the distilled water enters the sampling needle 71 of the sampling assembly 7 to clean the inner wall and the outer wall of the sampling needle 71. The specific cleaning process is the same as above. And because the cleaning valve 72 is communicated with the waste liquid bottle 34, the waste liquid is finally all sucked into the waste liquid bottle 34. Synchronously, after the distilled water enters the main port of the second switching valve 36, it directly enters the cathode cell 4 through the pipeline to clean the cathode cell 4. The distilled water in the cathode cell 4 gradually increases and submerges the port of the capillary. At this time, the first solenoid valve 42 connected to the positive pressure pipe 26 on the cathode cell 4 is opened. Through the positive pressure effect, the air pressure in the cathode cell 4 is slightly higher than the standard atmospheric pressure, and part of the distilled water will be pressed into the capillary to clean the capillary. The other end of the capillary is above the anode cup 5, and the overflowed distilled water in the capillary will drip into it. After the cleaning is completed, the first solenoid valve 42 connected to the waste liquid bottle 34 on the cathode cell 4 is opened, and the air pressure in the positive pressure pipe 26 is adjusted through the gas path system 2 so that the air pressure in the cathode cell 4 is less than the standard atmospheric pressure at this time. Therefore, all the waste liquid in the capillary will enter the cathode cell 4, and all the waste liquid is extracted into the waste liquid bottle 34 through the first solenoid valve 42 connected to the waste liquid bottle 34; Meanwhile, the distilled water entering the second solenoid valve 52 will directly fall inside the anode cup 5 to clean the anode cup 5. After the cleaning is completed, all the waste liquid is extracted into the waste liquid bottle 34 through the second solenoid valve 52 connected to the waste liquid bottle 34.

[0067] Step 3: The buffer solution in the buffer solution bottle 31 of the liquid path system 3 enters the cathode cell 4 and the anode cup 5, and gradually enters the capillary of the electrophoresis module connected to the cathode cell 4; Specifically: The gas path system 2 presses the buffer solution into the second switching valve 36 and the second solenoid valve 52 connected to the buffer solution bottle 31 respectively through the positive pressure effect; The buffer solution entering the second switching valve 36 from the sub-port will enter the main port pipeline of the second switching valve 36, and then directly enter the cathode cell 4 through the main port pipeline; The buffer solution in the cathode cell 4 gradually increases and submerges the port of the capillary. At this time, the first solenoid valve 42 connected to the positive pressure pipe 26 on the cathode cell 4 is opened. Through the positive pressure effect, the air pressure of the cathode cell 4 is slightly higher than the standard atmospheric pressure, and the buffer solution will be pressed into the capillary and gradually fill the capillary. The other end of the capillary is above the anode cup 5, and the overflowed buffer solution in the capillary will drip into it without waste; meanwhile, the buffer solution entering the second solenoid valve 52 directly falls into the anode cup 5 and gradually fills the anode cup 5; The cathode cell 4 and the anode cup 5 respectively limit the liquid level height of the buffer solution inside them through the first high and low liquid level sensor 41 and the second high and low liquid level sensor 51, so that the liquid level height of the buffer solution is within a suitable range. At this time, the ports at both ends of the capillary are respectively below the liquid level of the buffer solution inside the cathode cell 4 and the anode cup 5.

[0068] Step 4: Sampling work: Activate the sampling mechanism 7. The sampling needle 71 extends out of the sampling assembly 7. Use the plunger pump 6 to sample the blood sample tube 11 and inject the blood sample into the corresponding cavity of the dilution cup 12. Specifically: The sampling needle 71 moves downward in the vertical direction and extends into the first blood sample tube 11. Use the plunger pump 6 to generate negative pressure, and draw a part of the blood sample with the sampling needle 71. Then the sampling needle 71 moves upward and moves out of the blood sample tube 11. The entire sampling assembly 7 moves horizontally, so that the sampling needle 71 moves above the first cavity of the dilution cup 12. Then the sampling needle 71 descends, and the lower end of the sampling needle 71 enters the cavity of the dilution cup 12. Use the plunger pump 6 to generate positive pressure and extrude a part of the blood sample into the dilution cup 12.

[0069] Step 5: The sampling needle 7 returns inside the sampling assembly 7. Use the distilled water in the distilled water bottle 33 to wash the inner wall of the sampling needle 71, wash the outer wall of the sampling needle 71, and discharge the waste liquid into the waste liquid bottle 34. Specifically: The sampling needle 71 moves upward so that its lower port is inside the opening of the valve seat 73. The gas path system presses the distilled water into the main port pipeline of the first switching valve 35 through the branch port. And the branch port where the first switching valve 35 switches to work. Under the positive pressure of the plunger pump 6, use the main port of the first switching valve 35 to press the distilled water into the sampling needle 71 through the branch port that enters the working state at this time to wash the inner wall and outer wall of the sampling needle 71. The specific cleaning process is the same as in Step 2. And because the cleaning valve 72 is connected to the waste liquid bottle 34, all the waste liquid is finally sucked into the waste liquid bottle 34.

[0070] Step 6: Repeat Step 4 and Step 5 to complete the sampling of all blood sample tubes 12 on the sample rack 1 in sequence. Specifically: By moving the sampling needle 7 horizontally and cooperating with the vertical movement of the sampling needle 71, the sampling process of the blood sample in the blood sample tube 11 is realized, and the sampled blood sample is dropped into the corresponding dilution cup 12. After each sampling of a blood sample tube 11 is completed, it is necessary to wash the sampling needle 71 with distilled water to avoid mixing of blood samples and affecting the test results.

[0071] Step 7: Electrophoresis work: Place the dilution cup 12 after sampling under the electrophoresis module. Use the capillary to extend into the corresponding cavity in sequence to draw the blood sample, remove the dilution cup 12, and insert the capillary into the anode cup 5. Specifically: The anode cup 5 moves downward a certain distance, away from the capillary and the pipeline of the second solenoid valve 52, then moves horizontally and deviates from directly below the capillary. Then remove the sampled dilution cup 12 and place it directly below the capillary, and move upward so that the capillary extends into the corresponding cavity of the dilution cup 12 in sequence. The branch port of the first switching valve 35 communicating with the cathode cell 4 switches to work, generating negative pressure through the plunger pump 6, so that the air pressure in the cathode cell 4 is slightly less than the standard atmospheric pressure at this time. At this time, the capillary tube extending into the dilution cup 12 will extract part of the blood sample under the action of negative pressure; Then the dilution cup 12 descends and moves away from directly below the capillary tube, and the anode cup 5 moves back to directly below the capillary tube and rises a certain height to ensure that the end of the capillary tube extends into the anode cup 5 to facilitate the subsequent electrophoresis process.

[0072] Step eight: Perform electrophoresis separation on the blood sample in the capillary tube through the electrophoresis module to obtain the content of various proteins in the blood sample; Specifically: An electric field is generated by applying a voltage to perform the electrophoresis process. The electrophoresis process is a known technology for those skilled in the art, and the specific principle will not be elaborated too much. Through electrophoresis, the content of various proteins in the blood sample can be obtained, thereby reflecting the health status of the human body.

[0073] Step nine: Final work: Aspirate the waste liquid in the cathode cell 4, anode cup 5, and capillary tube after electrophoresis into the waste liquid bottle 34; Specifically: Both first solenoid valves 42 of the cathode cell 4 are opened, and the positive pressure generated by the gas circuit system 2 directly acts inside the cathode cell 4. Coupled with the suction of the negative pressure in the waste liquid bottle 34, the waste liquid inside the cathode cell 4 can be discharged more quickly; At the same time, the anode cup 5 aspirates the waste liquid through the pipeline extending to the bottom of the anode cup 5 connected to the waste liquid bottle 34 through the second solenoid valve 52, and quickly discharges the waste liquid into the waste liquid bottle; The flow direction of the waste liquid in the capillary tube is: It drips from the capillary tube into the cathode cell 4 and is discharged together with the waste liquid in the cathode cell 4. The reason is that under the action of the gas circuit system 2, the air pressure in the cathode cell 4 is slightly less than the standard atmospheric pressure, and the waste liquid in the capillary tube is extruded out of the capillary tube and enters the cathode cell 4, thereby realizing the discharge of the waste liquid in the capillary tube.

[0074] Step ten: Clean the capillary tube with the cleaning liquid in the cleaning liquid bottle 32 of the liquid circuit system 3 and discharge the waste liquid into the waste liquid bottle. Then, rinse the cathode cell 4, anode cup 5, and capillary tube as a whole with distilled water in the distilled water bottle 33 and discharge the waste liquid into the waste liquid bottle 34.

[0075] Specifically: Since the waste liquid in the capillary enters the cathode cell 4 from the capillary and then is discharged together, it is necessary to clean the cathode cell 4 and the capillary with a cleaning liquid to ensure complete cleaning to avoid affecting subsequent test results. At this time, the gas path system 2 operates, and the positive pressure port of the air pump 21 generates positive pressure. The cleaning liquid is pressed into the main port of the second switching valve 36 through the positive pressure pipe by the cleaning liquid 32, and then directly enters the cathode cell 4 through the pipeline to clean the cathode cell 4. The cleaning liquid in the cathode cell 4 gradually increases and submerges the port of the capillary. At this time, the first solenoid valve 42 connected to the positive pressure pipe 26 on the cathode cell 4 is opened. Through the positive pressure effect, the air pressure in the cathode cell 4 is slightly higher than the standard atmospheric pressure, and part of the cleaning liquid will be pressed into the capillary to clean the capillary. And since the other end of the capillary is above the anode cup 5, the cleaning liquid overflowing from the capillary will drip into it and will not drip on other structures to cause pollution. After the cleaning is completed, the first solenoid valve 42 connected to the waste liquid bottle 34 on the cathode cell 4 is opened. At the same time, under the adjustment of the gas path system 2, the air pressure in the cathode cell 4 is slightly less than the standard atmospheric pressure, and all the waste liquid in the cathode cell 4 and the capillary is pumped into the waste liquid bottle 34; Then, the cathode cell 4, the anode cup 5, and the capillary are rinsed again with distilled water. The specific steps are the same as in Step 2. The waste liquid from the secondary rinse is also all discharged into the waste liquid bottle 34. The above steps can be repeated subsequently to achieve continuous testing.

[0076] The above embodiments are only used to illustrate the technical idea of the present invention and cannot be used to limit the protection scope of the present invention. Any changes made on the basis of the technical solution according to the technical idea proposed by the present invention fall within the protection scope of the present invention.

Claims

1. A method for hemoglobin electrophoresis detection, characterized in that: The following steps are involved: Step 1: Preparation: Assemble the sample rack (1), the dilution cup (12) and the blood sample tube (11) and place them under the sampling mechanism (7), and add a hemolytic agent into the cavity of the dilution cup (12); Step 2: Start the gas system (2) and the liquid system (3), flush the electrophoresis module, the cathode tank (4), the anode cup (5) and the sampling assembly (7) with distilled water in the distilled water bottle (33) of the liquid system (3), and discharge the waste liquid into the waste liquid bottle (34) of the liquid system (3); Step 3: The buffer solution in the buffer solution bottle (31) of the liquid path system (3) enters the cathode tank (4) and the anode cup (5), and gradually enters the capillary of the electrophoresis module connected to the cathode tank (4); Step 4: Sampling: Start the sampling mechanism (7), the sampling needle (71) extends out of the sampling assembly (7), samples the blood sample tube (11) through the plunger pump (6), and injects the blood sample into the corresponding cavity of the dilution cup (12); Step 5: The sampling needle (7) returns to the interior of the sampling assembly (7), and the inner wall of the sampling needle (71) is rinsed with distilled water in the distilled water bottle (33), and the outer wall of the sampling needle (71) is rinsed, and the waste liquid is discharged into the waste liquid bottle (34); Step 6: Repeat steps 4 and 5 to complete sampling of all blood sample tubes (12) in sequence; Step 7: Electrophoresis: Place the dilution cup (12) after sampling under the electrophoresis module, insert the capillary tube into the corresponding cavity to extract the blood sample, remove the dilution cup (12), and insert the capillary tube into the anode cup (5); Step 8: Perform electrophoresis separation on the blood sample in the capillary tube through the electrophoresis module to obtain the content of various proteins in the blood sample; Step 9: Finishing work: After the electrophoresis is completed, the waste liquid in the cathode tank (4), the anode cup (5) and the capillary tube is sucked into the waste liquid bottle (34); Step 10: The capillary is cleaned with the cleaning liquid in the cleaning liquid bottle (32) of the liquid system (3) and the waste liquid is discharged into the waste liquid bottle. Then, the cathode tank (4), the anode cup (5) and the capillary are rinsed as a whole with the distilled water in the distilled water bottle (33) and the waste liquid is discharged into the waste liquid bottle (34).

2. A method for hemoglobin electrophoresis detection according to claim 1, characterized in that: The blood sample test tube (11) is arranged in an opening of the sample rack (1), and the dilution cup (12) is clamped on the outside of the sample rack (1). The number of cavities of the dilution cup (12) on the same sample rack (1) is the same as the number of blood sample test tubes (12).

3. A method for hemoglobin electrophoresis detection according to claim 1, characterized in that: The gas circuit system (2) comprises an air pump (21), an air circuit unit (22), an air storage tank (23), a safety valve (24), a plurality of negative pressure tubes (25) and a positive pressure tube (26); the negative pressure port of the air pump (21) is connected to the air circuit unit (22) via a negative pressure tube (25); the air circuit unit (22) is connected to a waste liquid bottle (34) via another negative pressure tube (25); the positive pressure port of the air pump (21) is connected to the air circuit unit (22) via a positive pressure tube (26); the air circuit unit (22) is respectively connected to a buffer solution bottle (31), a cleaning solution bottle (32) and a distilled water bottle (33) via a positive pressure tube (26) with a safety valve (24); and the air storage tank (22) for stabilizing pressure is connected to the air circuit unit (22) via a positive pressure tube (26).

4. A method for hemoglobin electrophoresis detection according to claim 1, characterized in that: The liquid circuit system (3) further comprises a first switching valve (35) and a second switching valve (36); the main port of the first switching valve (35) is connected to the plunger pump (6); the four sub-ports of the first switching valve (35) are respectively connected to the distilled water bottle (33), the cathode tank (4), the sampling needle (71) and the closed standby pipeline; the main port of the second switching valve (36) is connected to the cathode tank (4); the four sub-ports of the second switching valve (36) are respectively connected to the buffer bottle (31), the cleaning liquid bottle (32), the distilled water bottle (33) and the waste liquid bottle (34).

5. A method for hemoglobin electrophoresis detection according to claim 4, characterized in that: The cathode slot (4) is a closed space, the internal cavity of the cathode slot (4) is inclined, a plurality of first solenoid valves (42) connected to the internal cavity are arranged on the cathode slot (4), the first solenoid valve (42) located at the uppermost end of the inclined cavity is connected to the gas path unit (22) via a positive pressure pipe (26), the second solenoid valve (42) located at the lowermost end of the inclined cavity is connected to the waste liquid bottle (34) via a pipeline, a first high and low liquid level sensor (41) is arranged on the cathode slot (4), the first high and low liquid level sensor (41) extends into the cavity, and a capillary cluster head inlet (43) is also arranged above the cathode slot (4).

6. A method for hemoglobin electrophoresis detection according to claim 5, characterized in that: The connection ports of the first switching valve (35) and the second switching valve (36) and the cathode tank (4) are both located above the internal cavity.

7. A method for hemoglobin electrophoresis detection according to claim 1, characterized in that: The opening of the anode cup (5) faces upwards, and a plurality of second solenoid valves (52) are arranged above the opening of the anode cup (5). A pipe below the second solenoid valve (52) connected to the waste liquid bottle (34) extends into the bottom of the anode cup (5). A pipe below the second solenoid valve connected to the buffer bottle (31) and the distilled water bottle (33) is located above the anode cup (5). Second high and low liquid level sensors (51) are arranged on both sides of the second solenoid valve (52) and extend into the interior of the anode cup (5).

8. A method for hemoglobin electrophoresis detection according to claim 4, characterized in that: The sampling assembly (7) comprises a sampling needle (71), a cleaning valve (72) and a valve seat (73); a hole is opened in a vertical direction on the valve seat (73); the cleaning valve (72) is arranged on one side of the valve seat (73) and is connected to the opening of the valve seat (73); the cleaning valve (72) is connected to the waste liquid bottle (34) through a pipeline; the sampling needle (71) passes through the opening of the valve seat (73); and the upper end of the sampling needle (71) is connected to the branch port of the first switching valve (35) through a pipeline with a liquid level sensor.

9. A method for hemoglobin electrophoresis detection according to claim 9, characterized in that: The sampling component (7) can move in a horizontal direction, and the sampling needle (71) can move in a vertical direction.

10. A method for hemoglobin electrophoresis detection according to claim 9, characterized in that: The opening diameter is slightly larger than the outer diameter of the sampling needle (71).

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