Method for simulating digestion of pine pollen based on dynamic human body bionic digestion system

Abstract

The invention relates to the technical field of food, in particular to a method for simulating digestion of pine pollen based on a dynamic human body bionic digestive system, which comprises the following steps: S1, preparing digestive juice simulation liquid which comprises oral cavity digestive juice, stomach digestive juice and intestinal digestive juice; s2, preparing a pine pollen sample and simulating oral digestion; s3, starting a dynamic human body bionic digestion system, preheating, adding the pollen pini subjected to oral digestion treatment into the system, gradually and quantitatively adding gastric digestive juice and intestinal digestive juice into the system, and simulating a dynamic gastrointestinal digestion process; and S4, collecting gastrointestinal digestion products, and carrying out quick-freezing and freeze-drying treatment on the digestion products. The method for simulating the digestion of the pine pollen based on the dynamic human body bionic digestion system, provided by the invention, can be used for quickly, simply and conveniently simulating the process of digesting the pine pollen by people at low cost, and has important significance for researching the digestion and release process of the nutritional ingredients of the pine pollen in a human body and the change rule of the nutritional ingredients.

Description

Technical Field

[0001] The present invention relates to the technical field of food, and in particular to a method for simulating the digestion of pine pollen based on a dynamic human bionic digestive system. Background Art

[0002] Pine pollen is the sperm cell of pine plants and is a traditional pollen variety with both medicinal and edible uses. Because it is rich in more than 200 kinds of nutrients such as proteins, unsaturated fatty acids, vitamins, minerals, polyphenols and flavonoids, it is known as a natural "micro-nutrient library", with comprehensive, balanced and reasonable nutrition, and has extremely high nutritional and edible value and medicinal and health care functions. Domestic and foreign research shows that pine pollen has a variety of physiological effects, such as antioxidant, anti-inflammatory, regulating blood sugar, blood lipids and enhancing immunity, etc., and has broad development prospects, and is attracting more and more attention from the food and pharmaceutical industries.

[0003] However, the cell wall structure of pine pollen is firm, and the sporopollenin it contains has the characteristics of being resistant to acids and alkalis, high temperatures and high pressures, and remains stable under the action of digestive system enzymes. These factors may cause the cell wall of pine pollen to hinder the effective release, digestion and absorption of its nutrients in the human gastrointestinal tract. Therefore, it is urgent to scientifically evaluate the characteristics of pine pollen in human digestion and absorption.

[0004] At present, in-depth research on the digestion process in the real human body is still restricted by many factors such as ethics. Common research methods for in vivo digestion can be divided into in vivo digestion in animals and in vitro simulated digestion. Although in vivo digestion in animals can more truly reflect the digestive changes of food in the gastrointestinal tract, it is restricted by the ethical constraints of experimental animals and has many limitations such as high cost and great individual differences. Therefore, in vitro simulated digestion methods are often used. In vitro simulated digestion is divided into static digestion, semi-dynamic digestion and dynamic bionic digestion. Compared with static digestion and semi-dynamic digestion, dynamic bionic digestion can achieve gastrointestinal morphological bionics, increase the gastric peristaltic extrusion and continuous gastric emptying of the digestive sample, and the digestive fluid is dynamically secreted during the digestion process to automatically adjust the pH value of the gastric environment, which is closer to the real human digestive process.

[0005] In view of this, the present invention is specifically proposed. Summary of the Invention

[0006] The purpose of the present invention is to provide a method for simulating the digestion of pine pollen based on a dynamic human bionic digestive system, which can simulate the human digestion process of pine pollen quickly, simply and at low cost, and is of great significance for studying the digestion and release process of the nutrients of pine pollen in the human body and the change law of nutrients.

[0007] In the first aspect of the present invention, there is provided a method for simulating the digestion of pine pollen based on a dynamic human bionic digestive system, including the following steps:

[0008] S1. Prepare a digestive juice simulation solution, which includes oral digestive juice, gastric digestive juice, and intestinal digestive juice;

[0009] S2. Prepare the pine pollen sample and simulate oral digestion;

[0010] S3. Start the dynamic human bionic digestive system. After preheating, add the pine pollen processed by oral digestion into the system, and then gradually add the gastric digestive juice and intestinal digestive juice into the system in a quantitative manner to simulate the dynamic gastrointestinal digestion process;

[0011] S4. Collect the gastrointestinal digestion products and perform quick freezing and freeze-drying treatments on the digestion products.

[0012] Preferably, in step S1, the oral digestive juice is prepared from KCl, NaHCO 3 、KH 2 PO 4 、(NH 4 ) 2 CO 3 、MgCl 2 (H 2 O) 6 、CaCl 2 (H 2 O) 2 、α-amylase, and HCl as raw materials; the gastric digestive juice is prepared from NaCl, KCl, NaHCO 3 、KH 2 PO 4 、(NH 4 ) 2 CO 3 、MgCl 2 (H 2 O) 6 、CaCl 2 (H 2 O) 2 、pepsin, and HCl as raw materials; the intestinal digestive juice is prepared from NaCl, KCl, NaHCO 3 、KH 2 PO 4 、MgCl 2 (H 2 O) 6 、CaCl 2 (H 2 O) 2 、pancreatin, bile salts, and HCl as raw materials.

[0013] Preferably, the oral digestive juice includes: 14 - 16 mM KCl, 12 - 14 mM NaHCO 3 、3 - 4 mM KH 2 PO4 , 0.04 - 0.08 mM (NH 4 ) 2 CO 3 , 0.1 - 0.2 mM MgCl 2 (H 2 O) 6 , 1 - 2 mM CaCl 2 (H 2 O) 2 , 5 - 200 U / ml α - amylase, pH value is 6.5 - 7.5; The gastric digestive fluid includes: 46 - 48 mM NaCl, 6 - 8 mM KCl, 24 - 26 mM NaHCO 3 , 0.5 - 1.5 mM KH 2 PO 4 , 0.4 - 0.6 mM (NH 4 ) 2 CO 3 , 0.08 - 0.15 mM MgCl 2 (H 2 O) 6 , 0.1 - 0.2 mM CaCl 2 (H 2 O) 2 , 100 - 4000 U / ml pepsin, pH value is 1 - 3; The intestinal digestive fluid includes: 37 - 39 mM NaCl, 6 - 8 mM KCl, 83 - 85 mM NaHCO 3 , 0.5 - 1 mM KH 2 PO 4 , 0.2 - 0.5 mM MgCl 2 (H 2 O) 6 , 0.5 - 0.8 mM CaCl 2 (H 2 O) 2 , 0.1 - 6 USP / ml pancreatin, 5 - 25 mM bile salts, pH value is 6.5 - 7.5.

[0014] Preferably, step S2 includes: placing pine pollen, water and oral digestive fluid in a 37°C water bath for preheating. After preheating, quickly stir the three evenly until it becomes ketchup - like, and then put it into a 37°C magnetic - stirring water bath for water - bath stirring to simulate oral digestion.

[0015] Preferably, the mass ratio of the pine pollen, water and oral digestive fluid is (5 - 7):(13 - 15):(9 - 11).

[0016] Preferably, step S3 includes: starting the dynamic human bionic digestive system, preheating it to 37°C, adding a certain amount of gastric digestive juice to the system in advance, then adding the sample after oral digestion treatment into the bionic esophagus of the system, and continuously and slowly pumping gastric digestive juice and intestinal digestive juice into the gastric model and intestinal model of the system at the flow rate of the preset parameters to fully simulate the dynamic gastrointestinal digestion process of the human body digesting food.

[0017] In a specific embodiment, the dynamic human bionic digestive system is the dynamic bionic gastrointestinal digestion equipment, DHSI-IV, produced by Xiaodong Yijian (Suzhou) Instrument and Equipment Co., Ltd.

[0018] Preferably, in step S3, the parameters of the dynamic human bionic digestive system are set as follows: temperature: 37°C; gastric peristalsis frequency: 2 times / min; gastric pylorus opening size: 0 - 2 mm; gastric pylorus opening frequency: 2 times / min; gastric digestive juice secretion rate: 1.2 - 20 ml / min; hydrochloric acid secretion rate: adjusted according to pH value; gastric tilt angle: -0.4 - 2° / min; intestinal peristalsis frequency: 100 mm / min; intestinal digestive juice secretion rate: 1.2 - 2.2 ml / min; NaHCO3 secretion rate: 0.4 - 0.5 ml / min; total bionic gastric digestion time: 2 h; total bionic intestinal digestion time: 3 h.

[0019] Preferably, step S4 includes: collecting the digestion products from the bionic stomach and the end of the small intestine of the dynamic human bionic digestive system at different time periods, quickly freezing the collected digestion products with liquid nitrogen, and then performing freeze-drying treatment.

[0020] Preferably, the pine pollen includes any one of unbroken pine pollen and broken pine pollen.

[0021] In the second aspect of the present invention, an application of the method for simulating the digestion of pine pollen based on the dynamic human bionic digestive system is provided, and it is applied to the detection of the changes in the nutrient components of unbroken pine pollen and broken pine pollen.

[0022] The present invention has at least the following beneficial effects:

[0023] (1) The digestive juice simulation liquid in the present invention can be flexibly prepared according to the digestive juice components of different age groups (infants, adults, the elderly, etc.), and can specifically simulate the change rules of nutrient components in the process of different age groups digesting pine pollen.

[0024] (2) The present invention creatively adopts the scheme of synchronizing the preparation of the pine pollen sample and oral digestion, ensuring that the sample can be fully mixed with the digestive juice in the oral simulation digestion stage, and can accurately record the simulated oral digestion time; at the same time, the sample after oral digestion presents a state similar to ketchup, with good fluidity and gastrointestinal digestibility.

[0025] (3) When preparing the simulated solutions of oral, gastric, and intestinal digestive juices in the present invention, they are formulated strictly in accordance with the characteristics of human physiological conditions. At the same time, the preparation of each digestive juice is based on the active concentration of digestive enzymes, abandoning the traditional practice of using only the weight of digestive enzyme reagents as the preparation standard, thus solving the problem that it is difficult to repeat experimental results due to the differences between batches of digestive enzymes; this design method can accurately quantify the enzyme activity after the digestive juice is mixed with the pine pollen sample, laying a foundation for the quantitative research on the nutritional components of unbroken pine pollen and broken pine pollen during the digestion process; this method shows extremely high sensitivity, and during the actual digestion process of comparing unbroken pine pollen and broken pine pollen, it can also clearly reveal the differences in nutritional components between the two during the digestion stage.

[0026] (4) In the processing stage of digestion products in the present invention, to ensure that the nutritional components of pine pollen are not damaged, while inactivating digestive enzymes, the traditional methods such as heating in a water bath at high temperature, adjusting the pH value, or using relevant enzyme-inactivating reagents are abandoned, and instead, quick freezing is used to achieve the effect of inactivating enzymes; thereafter, the sample is further subjected to freeze-drying treatment, maintaining a low-temperature environment throughout the process to prevent the nutritional components in pine pollen from being damaged by artificial external conditions or changing due to microbial contamination.

[0027] (5) The digestion method proposed in the present invention can ensure that all nutritional components in pine pollen are fully digested. For example, for protein, within the first 2 hours of digestion, the protein digestion rate gradually increases, but during the period from 2 hours to 3 hours of digestion, its digestion rate tends to be stable and no longer increases, indicating that the protein is fully digested.

[0028] (6) Compared with static digestion and semi-dynamic digestion, the dynamic human bionic digestive system used in the present invention can achieve gastrointestinal morphological bionics, increase the gastric peristaltic extrusion and continuous gastric emptying of the digestive sample, the digestive juice is dynamically secreted during the digestion process, automatically adjusting the pH value of the gastric environment, and the pH value change in the stomach is close to the real human digestion process.

[0029] (7) Compared with in vivo digestion in animals, the present invention has the advantages of simple operation, good repeatability, low cost, and no ethical and moral constraints. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] In order to more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the following will briefly introduce the drawings required for the description of the specific embodiments or the prior art. Obviously, the drawings in the following description are some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0031] Figure 1Flow chart of the method for simulating the digestion of pine pollen based on a dynamic human bionic digestive system provided by the present invention.

[0032] Figure 2 Graph of the secretion rate of simulated gastric digestive juice provided by the present invention.

[0033] Figure 3 Graph of the secretion rate of simulated intestinal digestive juice provided by the present invention.

[0034] Figure 4 Graph of the pH change in the stomach during digestion provided by the present invention.

[0035] Figure 5 Graph of the gastric emptying curves of unbroken pine pollen and broken pine pollen provided by the present invention.

[0036] Figure 6 Graph of the protein digestion rate results of unbroken pine pollen and broken pine pollen at different time periods in the gastrointestinal tract provided by the present invention. Detailed implementation manners

[0037] It should be noted that the following detailed description is exemplary and is intended to provide further illustration of the present application. Unless otherwise specified, all technical and scientific terms used herein have the same meaning as commonly understood by those of ordinary skill in the technical field to which the present application belongs.

[0038] It should be noted that the terms used herein are only for describing specific implementation manners and are not intended to limit the exemplary implementation manners according to the present application. As used herein, unless the context clearly indicates otherwise, the singular forms also include the plural forms. In addition, it should be understood that when the terms "comprise" and / or "include" are used in this specification, they indicate the presence of the described features, steps, operations, devices, components, and / or their combinations.

[0039] The technical solutions of the present invention will be clearly and completely described below in conjunction with the embodiments. Obviously, the described embodiments are some, but not all, of the embodiments of the present invention. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0040] Embodiment

[0041] As Figure 1 shown, this embodiment takes the simulation of the digestion of pine pollen by the elderly as an example to provide a method for simulating the digestion of pine pollen based on a dynamic human bionic digestive system, including the following steps:

[0042] S1. Prepare a simulated digestive fluid for the elderly, which includes oral digestive fluid, gastric digestive fluid, and intestinal digestive fluid.

[0043] In the simulated digestive fluid after preparation, the oral digestive fluid includes: 15.11 mM KCl, 13.6 mM NaHCO 3 , 3.7 mM KH 2 PO 4 , 0.06 mM (NH 4 ) 2 CO 3 , 0.15 mM MgCl 2 (H 2 O) 6 , 1.5 mM CaCl 2 (H 2 O) 2 , 150 U / ml α-amylase, and the pH is adjusted to 7 with 1 M hydrochloric acid; the gastric digestive fluid includes: 47.25 mM NaCl, 6.9 mM KCl, 25 mM NaHCO 3 , 0.9 mM KH 2 PO 4 , 0.5 mM (NH 4 ) 2 CO 3 , 0.12 mM MgCl 2 (H 2 O) 6 , 0.15 mM CaCl 2 (H 2 O) 2 , 3000 U / ml pepsin, and the pH is adjusted to 2 with 6 M hydrochloric acid; the intestinal digestive fluid includes: 38.44 mM NaCl, 6.8 mM KCl, 84.99 mM NaHCO 3 , 0.8 mM KH 2 PO 4 , 0.33 mM MgCl 2 (H 2 O) 6 , 0.6 mM CaCl 2 (H 2 O) 2 , 5 USP / ml pancreatin, 13.35 mM bile salts, and the pH is adjusted to 6.8 with 6 M hydrochloric acid.

[0044] S2. Prepare the pine pollen sample and simulate oral digestion: Weigh 60 g of pine pollen to be subjected to dynamic bionic digestion, 140 g of ultrapure water, and 100 g of simulated oral digestive fluid respectively. Place the three in a water bath at 37 °C and preheat for 5 min. After preheating, quickly stir the three evenly until it becomes ketchup-like, and immediately put it into a magnetic stirring water bath at 37 °C and stir for 2 min to simulate oral digestion.

[0045] S3. Start the dynamic human bionic digestive system and preheat it to 37°C. Add 20 ml of reserved gastric digestive fluid to the dynamic human bionic digestive system to ensure that the low pH environment in the stomach can be fully simulated at the beginning of digestion. Subsequently, add 300 g of the sample after oral digestion treatment to the bionic esophagus. According to the flow rate of the preset parameters (such as Figure 2-3 shown), continuously and slowly pump gastric digestive fluid and intestinal digestive fluid into the gastric model and intestinal model of the system to fully simulate the dynamic gastrointestinal digestion process of the human body digesting food.

[0046] In this embodiment, the dynamic human bionic digestive system is the dynamic bionic gastrointestinal digestion equipment, DHSI-IV, produced by Xiaodong Yijian (Suzhou) Instrument Equipment Co., Ltd. The specific parameters are as follows:

[0047] Temperature: 37°C; gastric peristalsis frequency: 2 times / min; gastric pyloric opening size: 0 - 2 mm; gastric pyloric opening frequency: 2 times / min; gastric digestive fluid secretion rate: 1.2 - 20 ml / min; hydrochloric acid secretion rate: automatically adjusted according to the set pH value; gastric tilt angle: -0.4 - 2° / min; intestinal peristalsis frequency: 100 mm / min; intestinal digestive fluid secretion rate: 1.2 - 2.2 ml / min; NaHCO 3 secretion rate: 0.4 - 0.5 ml / min; total bionic gastric digestion time: 2 h; total bionic intestinal digestion time: 3 h.

[0048] S4. After digestion starts, collect a certain volume of digestion products from the bionic stomach or the end of the small intestine at different times. Immediately use liquid nitrogen to quickly freeze the collected pine pollen digestion products to achieve the effect of inactivating enzymes, and then further perform freeze-drying treatment.

[0049] Comparative Example 1

[0050] This comparative example is basically the same as the embodiment. The difference is that in this comparative example, when preparing the pine pollen sample in step S2, 60 g of pine pollen to be subjected to dynamic bionic digestion, 140 g of ultrapure water, and 100 g of simulated oral digestive fluid are weighed respectively. The three are placed in a water bath at 37°C and preheated for 5 min. After preheating, 60 g of pine pollen and 140 g of ultrapure water are mixed and quickly stirred to prepare a pine pollen sample. Subsequently, 100 g of simulated oral digestive fluid is added, and it is immediately placed in a magnetic stirring water bath at 37°C and stirred in the water bath for 2 min to simulate oral digestion. The results show that: Given that the dietary fiber content of pine pollen is as high as 60%, it shows extremely high water absorption, resulting in the prepared pine pollen sample being prone to aggregation and presenting a muddy state, with a significant reduction in fluidity. When the oral digestive fluid is added, it is difficult to achieve uniform mixing with the pine pollen sample in a short time, so the oral digestion time cannot be accurately simulated.

[0051] Comparative Example 2

[0052] This comparative example is basically the same as the example, except that: in this comparative example, in step S4, a high-temperature water bath is used for heating to achieve the enzyme inactivation effect.

[0053] The results show that: when the collected pine pollen digestion product is immediately heated by a high-temperature water bath to inactivate the enzyme (100 °C, 10 min), the color of the digestion product will gradually change from yellow to tawny, indicating that some substances in the digestion product have undergone chemical transformation, which is not conducive to studying the changes in nutrients during the human digestion process of pine pollen. While the method of the present invention uses liquid nitrogen treatment, maintaining a low temperature (-196 °C) during the enzyme inactivation process, which will not damage the nutrients in pine pollen, facilitating the analysis and research of the digested samples. At the same time, the samples treated with liquid nitrogen are further freeze-dried at low temperature, maintaining a low temperature throughout the entire process of sample treatment and excluding the influence of temperature.

[0054] Detection Example

[0055] The method for simulating the digestion of pine pollen by the elderly based on a dynamic human bionic digestive system provided in the example is applied to the detection of changes in the nutrient components of unbroken pine pollen and broken pine pollen.

[0056] The pH change in the stomach during the digestion process is as Figure 4 shown. It can be seen from the figure that the pH value change in the stomach is close to the actual human digestion process.

[0057] The gastric emptying curves of unbroken pine pollen and broken pine pollen in the stomach are as Figure 5 shown. It can be seen from the figure the gastric peristaltic squeezing and continuous gastric emptying of the digestion samples.

[0058] The protein digestibility of unbroken pine pollen and broken pine pollen at different time periods in the stomach and intestine is as Figure 6 shown. It can be seen from the figure that within the first 2 hours of digestion, the protein digestibility gradually increases, but during the period from 2 hours to 3 hours of digestion, its digestibility tends to be stable and no longer increases, indicating that the protein is fully digested.

[0059] The detection results of each nutrient component are shown in Table 1-2.

[0060] Table 1 Results of simulating the oral and gastric digestion of unbroken pine pollen and broken pine pollen in the elderly

[0061]

[0062] Note: ND: Not detected.

[0063] Table 2 Results of simulating the oral, gastric, and intestinal digestion of unbroken pine pollen and broken pine pollen in the elderly

[0064]

[0065]

[0066] Note: ND: Not detected.

[0067] As can be seen from Table 1-2, the method for simulating the digestion of pine pollen based on a dynamic human bionic digestive system provided by the present invention can be well applied to the detection of changes in the nutrient components of unbroken pine pollen and broken pine pollen, and is used to compare the change rules of nutrient components in the process of human digestion of unbroken pine pollen and broken pine pollen, so as to promote the development of the pine pollen industry.

[0068] Finally, it should be noted that: the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that: they can still modify the technical solutions recorded in the foregoing embodiments, or perform equivalent replacements on some or all of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A method for simulating the digestion of pine pollen based on a dynamic human bionic digestive system, characterized in that: The steps include: S1. preparing a digestive fluid simulation solution, wherein the digestive fluid simulation solution includes: oral digestive fluid, gastric digestive fluid and intestinal digestive fluid; S2, prepare pine pollen samples and simulate oral digestion; S3, starting the dynamic human bionic digestion system, adding pine pollen that has been digested in the oral cavity into the system after preheating, and then gradually adding gastric digestive fluid and intestinal digestive fluid into the system in a quantitative manner to simulate the dynamic gastrointestinal digestion process; S4. Collect gastrointestinal digestion products, and perform quick freezing and freeze-drying treatment on the digestion products.

2. The method for simulating pine pollen digestion based on a dynamic human bionic digestive system according to claim 1, characterized in that: In step S1, the oral digestive juice is prepared with KCl, NaHCO3, KH2PO4, (NH4)2CO3, MgCl2(H2O)6, CaCl2(H2O)2, α-amylase, and HCl as raw materials; the gastric digestive juice is prepared with NaCl, KCl, NaHCO3, KH2PO4, (NH4)2CO3, MgCl2(H2O)6, CaCl2(H2O)2, pepsin, and HCl as raw materials; the intestinal digestive juice is prepared with NaCl, KCl, NaHCO3, KH2PO4, MgCl2(H2O)6, CaCl2(H2O)2, pancreatic enzyme, bile salt, and HCl as raw materials.

3. The method for simulating pine pollen digestion based on a dynamic human bionic digestive system according to claim 2, characterized in that: The oral digestive juice comprises: 14-16mM KCl, 12-14mM NaHCO3, 3-4mM KH2PO4, 0.04-0.08mM (NH4)2CO3, 0.1-0.2mM MgCl2(H2O)6, 1-2mM CaCl2(H2O)2, 5-200U / ml α-amylase, and the pH value is 6.5-7.5; the gastric digestive juice comprises: 46-48mM NaCl, 6-8mM KCl, 24-26mM NaHCO3, 0.5-1.5mM KH2PO4, 0.4-0.6mM (NH4)2CO3, 0.08-0.15mM MgCl2(H2O)6, 0.1-0.2mM CaCl2(H2O)2, 100-4000U / ml pepsin, pH value is 1-3; the intestinal digestive fluid includes: 37-39mM NaCl, 6-8mM KCl, 83-85mM NaHCO3, 0.5-1mM KH2PO4, 0.2-0.5mM MgCl2(H2O)6, 0.5-0.8mM CaCl2(H2O)2, 0.1-6USP / ml pancreatic enzyme, 5-25mM bile salt, pH value is 6.5-7.

5.

4. The method for simulating pine pollen digestion based on a dynamic human bionic digestive system according to claim 1, characterized in that: Step S2 includes: preheating pine pollen, water and oral digestive fluid in a 37°C water bath, and after preheating, quickly stirring the three to a ketchup-like state, placing them in a 37°C magnetic stirring water bath, and stirring in the water bath to simulate oral digestion.

5. The method for simulating pine pollen digestion based on a dynamic human bionic digestive system according to claim 4, characterized in that: The mass ratio of the pine pollen, water and oral digestive juice is (5-7):(13-15):(9-11).

6. The method for simulating pine pollen digestion based on a dynamic human bionic digestive system according to claim 1, characterized in that: Step S3 includes: starting the dynamic human bionic digestive system, preheating it to 37°C, adding a certain amount of gastric digestive fluid into the system in advance, then adding the sample after oral digestion into the bionic esophagus of the system, and continuously and slowly pumping gastric digestive fluid and intestinal digestive fluid into the stomach model and intestinal model of the system at a flow rate of preset parameters, so as to fully simulate the dynamic gastrointestinal digestion process of human body digesting food.

7. The method for simulating pine pollen digestion based on a dynamic human bionic digestive system according to claim 1, characterized in that: In step S3, the parameters of the dynamic human bionic digestive system are set as follows: temperature: 37°C; gastric peristalsis frequency: 2 times / min; gastric pyloric opening size: 0-2mm; gastric pyloric opening frequency: 2 times / min; gastric digestive juice secretion rate: 1.2-20ml / min; hydrochloric acid secretion rate: adjusted according to pH value; gastric inclination angle: -0.4-2° / min; intestinal peristalsis frequency: 100mm / min; intestinal digestive juice secretion rate: 1.2-2.2ml / min; NaHCO3 secretion rate: 0.4-0.5ml / min; bionic stomach total digestion time: 2h; bionic intestine total digestion time: 3h.

8. The method for simulating pine pollen digestion based on a dynamic human bionic digestive system according to claim 1, characterized in that: Step S4 includes: collecting digestion products from the bionic stomach and the end of the small intestine of the dynamic human bionic digestive system at different time periods, quick-freezing the collected digestion products with liquid nitrogen, and then freeze-drying them.

9. The method for simulating pine pollen digestion based on a dynamic human bionic digestive system according to claim 1, characterized in that: The pine pollen includes one or more of unbroken pine pollen and broken pine pollen.

10. Application of the method for simulating pine pollen digestion based on a dynamic human bionic digestive system according to any one of claims 1 to 9, characterized in that: It is applied to the detection of changes in nutritional components of unbroken pine pollen and broken pine pollen.

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