Manufacturing method of bletilla striata seed line

Abstract

The invention discloses a method for manufacturing a rhizoma bletillae seed line. The method comprises the following steps: preparing a sodium alginate solution and a calcium chloride solution; adding an additive into the sodium alginate solution to obtain a treated sodium alginate solution; the rhizoma bletillae seeds are put into the treated sodium alginate solution to be evenly stirred and then soaked; respectively pouring a calcium chloride solution and a sodium alginate solution containing rhizoma bletillae seeds into two containers; soaking the fine line in a calcium chloride solution; soaking the fully soaked thin threads in a treated sodium alginate solution containing bletilla striata seeds, taking out the thin threads, washing the thin threads with tap water, and absorbing moisture on the surfaces of the seeds coated with the thin threads with sterile paper; laying wet paper at the bottom of a culture appliance, and putting the fine line coated seeds into the culture appliance for culture. The bletilla striata seeds are wrapped with the thin lines, and the problems that the bletilla striata seeds are difficult to germinate and fixed-point sowing is conducted are solved.

Description

Technical Field

[0001] The present invention belongs to the technical field of Bletilla striata seeds, and particularly relates to a method for making Bletilla striata seed lines. Background Art

[0002] Bletilla striata Bletilla striata (Thunb. ex A.Murray) Rchb.f.), is a plant of the genus Bletilla in the Orchidaceae family, also known as Bletilla, Gan Gen, Bai Ji, Zhu Lan, Zi Lan, etc. The rhizome of Bletilla striata can be used as a traditional Chinese medicine, with the effects of astringing to arrest bleeding and detumescence and promoting granulation. It is mainly used to treat hemoptysis, hematemesis, traumatic bleeding, sores and ulcers, skin chaps, pulmonary tuberculosis hemoptysis, ulcer disease bleeding and other diseases. The flower color of Bletilla striata is bright and the shape is peculiar, which can be used as potted plants and flower arranging materials for ornamental purposes; the Bletilla striata gum in its stem has the effects of delaying aging, thickening, suspending, moisturizing, etc., and can be widely used in industries such as medicine and cosmetics.

[0003] Due to the expanding market demand for Bletilla striata, wild Bletilla striata in most parts of China has been over-excavated and is on the verge of extinction. In 2013, Bletilla striata was included in the "China Biodiversity Red List - Higher Plants Volume" in China and was rated as an endangered (EN) plant. In 2021, Bletilla striata was included in the protection list of "National Key Protected Wild Plants" in China and is a national second-class protected plant.

[0004] The seeds of Bletilla striata are as small as dust, without endosperm, and are very difficult to germinate and reproduce under natural conditions. Therefore, artificial rapid propagation of Bletilla striata seedlings has become the main way to solve the shortage of Bletilla striata resources. Summary of the Invention

[0005] To solve the above problems, the present invention provides a method for making Bletilla striata seed lines. Based on artificial seed technology, the Bletilla striata seeds are wrapped with thin lines, solving the problems of difficult germination and fixed-point sowing of Bletilla striata seeds.

[0006] The present invention is achieved through the following technical solutions: A method for making Bletilla striata seed lines, comprising the following steps: Step 1, prepare sodium alginate solution and calcium chloride solution; Step 2, add additives to the sodium alginate solution prepared in Step 1 to obtain a treated sodium alginate solution; Step 3, put the Bletilla striata seeds into the treated sodium alginate solution, stir evenly and soak; Step 4, pour the calcium chloride solution and the sodium alginate solution containing Bletilla striata seeds in Step 3 into two containers respectively; Step 5, take an appropriate length of thin line, immerse it in the calcium chloride solution, and soak it fully; Step 6: Put the fine thread fully soaked in the calcium chloride solution into the sodium alginate solution containing Bletilla striata seeds and soak it fully. Step 7: After soaking, lift the unsoaked end by hand to pull out the whole fine thread, rinse the pulled-out fine thread with water to terminate the ion reaction, obtain fine-thread coated seeds, and absorb the water on the surface of the fine-thread coated seeds with sterile paper. Step 8: Lay a layer of toilet paper and filter paper at the bottom of the culture apparatus, use a dropper to absorb distilled water to moisten the two layers of paper, and there is a water film at the bottom of the apparatus. Put the fine-thread coated seeds into the culture apparatus for cultivation.

[0007] Further, in Step 1, the concentration of the sodium alginate solution is 1%-2%.

[0008] Further, in Step 1, the concentration of the calcium chloride solution is 1%-4%.

[0009] Further, in Step 2, the additives include hormones, fertilizers, bacteriostatic agents, glucose, sucrose, coconut juice and activated carbon powder.

[0010] Further, the hormones include 0.5-1.5 mg / L GA, 0.05-0.15 mg / L 6-BA, 0.05-0.15 mg / L NAA; the fertilizers include 3-8 g / L water-soluble fertilizer (20-20-20); the concentration of the bacteriostatic agent is 0.5-2 g / L; the concentration of glucose is 3-8 g / L; the concentration of sucrose is 3-8 g / L; the concentration of coconut juice is 100-200 ml / L; the concentration of activated carbon powder is 0.5-1.5 g / L.

[0011] Further, when soaking in Step 6, use a glass rod or forceps to compact the fine thread so that the whole fine thread is fully soaked in the sodium alginate solution containing Bletilla striata seeds.

[0012] The beneficial effects of the present invention are as follows: 1. Through the present invention, the density of seeds on the line and the density of sowing and wiring can be adjusted, and the sowing position and sowing density of seeds can be controlled. 2. Through the method of the present invention, substances such as nutrients, hormones, pesticides, etc. required for the germination of Bletilla striata seeds can be directly provided, making the seed germination and seedling growth stronger. 3. It can shorten the seed germination time, improve the seed germination rate, and the seed germination time is shorter. Description of the Drawings

[0013] Figure 1 is a schematic diagram of spherical coated seeds of the prior art; Figure 2 is a schematic diagram of the fine-thread shaped coated seeds of the present invention; Figure 3 is an enlarged view of the fine-thread shaped coated seeds of the present invention. Specific implementation mode

[0014] A method for making Bletilla striata seed threads, comprising the following steps: Step 1, prepare a sodium alginate solution (1%-2%) and a calcium chloride solution (1%-4%); Step 2, add additives to the sodium alginate solution prepared in Step 1 to obtain a treated sodium alginate solution; The additives include hormones, fertilizers, bacteriostatic agents, glucose, sucrose, coconut juice and activated carbon. The hormones include 0.5-1.5 mg / L GA, 0.05-0.15 mg / L 6-BA, 0.05-0.15 mg / L NAA; the fertilizers include 3-8 g / L water-soluble fertilizer (20-20-20); the concentration of the bacteriostatic agent is 0.5-2 g / L; the concentration of glucose is 3-8 g / L; the concentration of sucrose is 3-8 g / L; the concentration of coconut juice is 100-200 ml / L; the concentration of activated carbon powder is 0.5-1.5 g / L.

[0015] Step 3, put the Bletilla striata seeds into the treated sodium alginate solution, stir evenly and soak for 4-6 minutes; Step 4, pour the treated sodium alginate solution containing Bletilla striata seeds and the calcium chloride solution into two identical containers respectively; Step 5, take an appropriate length of thin line, immerse it in the calcium chloride solution, and soak it fully for 2-3 minutes; Step 6, put the thin line fully soaked in the calcium chloride solution into the treated sodium alginate solution containing Bletilla striata seeds, press the thin line with a glass rod or forceps to make the whole thin line fully soak in the treated sodium alginate solution containing Bletilla striata seeds; Step 7, after soaking for 2-3 minutes, lift the unsoaked end by hand to pull out the whole thin line, rinse the pulled-out thin line under tap water to terminate the ion reaction, obtain the thin line coated seeds, and absorb the water on the surface of the thin line coated seeds with sterile paper; Step 8, lay a layer of toilet paper and filter paper at the bottom of the culture bottle, use a dropper to absorb distilled water to moisten the two layers of paper, and there is a water film at the bottom of the bottle, and put the thin line coated seeds into the culture bottle for cultivation.

[0016] The following further describes the present invention in conjunction with the accompanying drawings and specific preferred embodiments, but does not limit the protection scope of the present invention accordingly.

[0017] Example 1 A method for making Bletilla striata seed threads, comprising the following steps: Step 1, prepare a sodium alginate solution with a concentration of 1% and a calcium chloride solution with a concentration of 1%; Step 2: Add additives to the sodium alginate solution prepared in Step 1 to obtain a treated sodium alginate solution; The additives include hormones, fertilizers, bacteriostatic agents, glucose, sucrose, coconut juice, and activated carbon powder. The hormones include 1.0 mg / L GA, 0.1 mg / L 6-BA, and 0.1 mg / L NAA; the fertilizer includes 5 g / L water-soluble fertilizer (20-20-20); the concentration of the bacteriostatic agent is 1 g / L; the concentration of glucose is 5 g / L; the concentration of sucrose is 5 g / L; the concentration of coconut juice is 167 ml / L; the concentration of activated carbon powder is 1 g / L.

[0018] Step 3: Put the Bletilla striata seeds into the treated sodium alginate solution, stir evenly, and soak for 4 minutes; Step 4: Pour the treated sodium alginate solution containing Bletilla striata seeds and the calcium chloride solution into two identical culture dishes respectively; Step 5: Take a fine thread of appropriate length, immerse it in the calcium chloride solution, and soak it thoroughly for 2 minutes; Step 6: Put the fine thread that has been fully soaked in the calcium chloride solution into the treated sodium alginate solution containing Bletilla striata seeds, and use a glass rod or tweezers to press down the fine thread so that the whole fine thread is fully soaked in the treated sodium alginate solution containing Bletilla striata seeds; Step 7: After soaking for 2 minutes, lift the unsoaked end by hand to pull out the whole fine thread, rinse the pulled-out fine thread under tap water to terminate the ion reaction, obtain fine-thread coated seeds, and use sterile paper to absorb the moisture on the surface of the fine-thread coated seeds; Step 8: Lay a layer of toilet paper and filter paper at the bottom of the culture bottle, use a dropper to draw distilled water to moisten the two layers of paper, and there is a water film at the bottom of the bottle. Put the fine-thread coated seeds into the culture bottle for cultivation.

[0019] Example 2 A method for making Bletilla striata seed threads includes the following steps: Step 1: Prepare a sodium alginate solution with a concentration of 2% and a calcium chloride solution with a concentration of 4%; Step 2: Add additives to the sodium alginate solution prepared in Step 1 to obtain a treated sodium alginate solution; The additives include hormones, fertilizers, bacteriostatic agents, glucose, sucrose, coconut juice, and activated carbon powder. The hormones include 1.2 mg / L GA, 0.1 mg / L 6-BA, and 0.1 mg / L NAA; the fertilizer includes 3 g / L water-soluble fertilizer (20-20-20); the concentration of the bacteriostatic agent is 1.5 g / L; the concentration of glucose is 4 g / L; the concentration of sucrose is 4 g / L; the concentration of coconut juice is 150 ml / L; the concentration of activated carbon powder is 1 g / L.

[0020] Step 3: Put the Bletilla striata seeds into the treated sodium alginate solution, stir evenly and soak for 6 minutes; Step 4: Pour the treated sodium alginate solution containing Bletilla striata seeds and calcium chloride solution into two containers respectively; Step 5: Take an appropriate length of thin line, immerse it in the calcium chloride solution and soak it thoroughly for 3 minutes; Step 6: Put the thin line that has been fully soaked in the calcium chloride solution into the treated sodium alginate solution containing Bletilla striata seeds, and use a glass rod or tweezers to press down the thin line to make the whole thin line fully soak in the treated sodium alginate solution containing Bletilla striata seeds; Step 7: After soaking for 3 minutes, lift the unsoaked end by hand and pull out the whole thin line, rinse the pulled-out thin line under tap water to terminate the ion reaction, obtain the thin line-coated seeds, and use sterile paper to absorb the water on the surface of the thin line-coated seeds; Step 8: Lay a layer of toilet paper and filter paper at the bottom of the culture bottle, use a dropper to absorb distilled water to moisten the two layers of paper, and there is a water film at the bottom of the bottle, and put the thin line-coated seeds into the culture bottle for cultivation.

[0021] The germination rate of the Bletilla striata seeds cultivated by the method in the above embodiment reaches 78.9 - 90.4%. Moreover, by using this cultivation method, the density of seeds on the line and the density of sowing wiring can be adjusted, the sowing position and sowing density of seeds can be controlled, and the problems of difficult germination of Bletilla striata seeds and fixed-point sowing are solved.

[0022] It should be noted that although the present invention has been described by the above embodiments, the present invention can also have many other embodiments. Without departing from the spirit and scope of the present invention, those skilled in the art can obviously make various corresponding changes and deformations to the present invention, but these changes and deformations should all fall within the scope protected by the appended claims of the present invention and their equivalents.

Claims

1. A method for making a Bletilla striata seed line, characterized in that: The following steps are involved: Step 1, preparing sodium alginate solution and calcium chloride solution; Step 2, adding additives to the sodium alginate solution prepared in step 1 to obtain a treated sodium alginate solution; Step 3, put the Bletilla striata seeds into the treated sodium alginate solution, stir evenly and soak; Step 4: Pour the calcium chloride solution and the sodium alginate solution containing Bletilla striata seeds in step 3 into two containers respectively; Step 5: Take a thin wire of appropriate length and immerse it in the calcium chloride solution for full soaking; Step 6: After being fully soaked in the calcium chloride solution, the thin thread is placed in the sodium alginate solution containing Bletilla striata seeds and fully soaked; Step 7: After soaking, lift the unsoaked end by hand to pull out the whole thin wire, rinse the pulled thin wire with water to terminate the ion reaction, obtain the thin wire coated seeds, and absorb the moisture on the surface of the thin wire coated seeds with sterile paper; Step 8. Place a layer of toilet paper and filter paper on the bottom of the culture vessel. Use a rubber-tipped dropper to draw distilled water to moisten the two layers of paper and leave a layer of water film on the bottom of the vessel. Place the thread-coated seeds into the culture vessel for culture.

2. The method for making a Bletilla striata seed line according to claim 1, characterized in that: The concentration of the sodium alginate solution in step 1 is 1%-2%.

3. The method for making a Bletilla striata seed line according to claim 1, characterized in that: The concentration of the calcium chloride solution in step 1 is 1%-4%.

4. The method for making a Bletilla striata seed line according to claim 1, characterized in that: The additives in step 2 include hormones, fertilizers, antibacterial agents, glucose, sucrose, coconut milk and activated carbon powder.

5. The method for making a Bletilla striata seed line according to claim 4, characterized in that: The hormones include 0.5~1.5mg / L GA, 0.05~0.15mg / L 6-BA, and 0.05~0.15mg / L NAA; the fertilizer includes 3~8g / L water-soluble fertilizer; the antibacterial agent concentration is 0.5~2g / L; the glucose concentration is 3~8g / L; the sucrose concentration is 3~8g / L; the coconut milk concentration is 100~200ml / L; and the activated carbon powder concentration is 0.5~1.5g / L.

6. The method for making a Bletilla striata seed line according to claim 1, characterized in that: During the soaking in step six, use a glass rod or tweezers to compact the thin wire so that the entire thin wire is fully immersed in the sodium alginate solution containing Bletilla striata seeds.

Images