Production method of organic pollution-free OMG3 eggs

Abstract

The invention discloses a production method of organic pollution-free OMG3 eggs, and relates to the technical field of poultry farming, and the method comprises the following specific steps: S100, mixing schizochytrium limacinum dry powder with hermetia illucens larva protein powder to obtain a basic fermentation substrate, S200, inoculating a composite flora into the substrate, S300, carrying out two-stage dynamic fermentation, and S400, mixing fermentation products to prepare an OMG3 feed, according to the method, ultra-efficient conversion of an OMG3 precursor is achieved through the matching of schizochytrium limacinum and hermetia illucens protein in combination with stepped fermentation of lactic acid bacteria and alginic acid degrading bacteria, specifically, in the first-stage anaerobic fermentation, cell walls of the schizochytrium limacinum are softened by organic acid secreted by lactobacillus plantarum, the cell walls of the schizochytrium limacinum are softened by organic acid secreted by lactobacillus plantarum, and the cell walls of the OMG3 are softened by organic acid secreted by lactobacillus plantarum; in the first-stage aerobic fermentation, the ALA precursor is converted into active OMG3, anti-nutritional factors are degraded, and dual guarantee of OMG3 enrichment and feed safety is achieved.

Description

Technical Field

[0001] The invention relates to the technical field of poultry breeding, and in particular to a method for producing organic pollution-free OMG3 eggs. Background Art

[0002] With the growing demand for eggs with high nutritional value among consumers, OMG3 (Omega-3) fortified eggs have become a hot topic in the market. Traditional OMG3 egg production mainly involves adding fish oil or algae powder to laying hen feed, using the hens' metabolism to convert α-linolenic acid (ALA) into OMG3. However, fish oil has problems such as heavy metal enrichment and poor oxidative stability, and the ALA conversion rate is low, resulting in large fluctuations in the OMG3 content of eggs, making it difficult to meet high-end market demand. In addition, existing feed processes rely on chemical antioxidants and antibiotics to maintain stability, which is contrary to the requirements of organic pollution-free certification. There is an urgent need to develop safe and efficient OMG3 enrichment technology.

[0003] At present, in the existing egg production technology, in order to increase the OMG3 content in eggs, on the one hand, the traditional feed formula is mainly based on plant proteins such as soybean meal, which can meet the basic nutritional needs of chickens, but soybean meal contains more anti-nutritional factors, which affect the absorption and utilization of nutrients by chickens, and thus affect the quality of eggs; on the other hand, the existing fermentation feed technology has limited effect in increasing the OMG3 content in eggs, and the fermentation process is complicated, making it difficult to achieve large-scale production. At the same time, plant-derived OMG3 precursors such as linseed oil have low conversion efficiency and are easily oxidized and destroyed, affecting the quality of the final product. In addition, antibiotics are often used in the prevention and treatment of chicken diseases, which may lead to the presence of antibiotic residues in eggs, which do not meet the standards of pollution-free eggs and pose potential risks to human health.

[0004] In summary, the existing OMG3 sources have low bioavailability and poor stability; protein source replacement and anti-nutritional factor elimination have failed to form a synergistic solution; the fermentation process cannot take into account the efficient conversion of multiple target components. Therefore, an innovative method is urgently needed to break through the efficiency limitations of OMG3 biofortification and achieve full-chain optimization of clean feed production and healthy laying hen farming. Summary of the invention

[0005] The purpose of the present invention is to make up for the shortcomings of the prior art and provide a method for producing organic and pollution-free OMG3 eggs. The method can improve the conversion rate of ALA to OMG3 by two-stage dynamic fermentation of schizochytrium and black soldier fly larvae protein and utilizing the synergistic effect of lactic acid bacteria and alginate-degrading bacteria. During the fermentation process, the algae cell wall is efficiently degraded to stabilize the OMG3 content of eggs.

[0006] To solve the above technical problems, the present invention provides the following technical solution: A production method of organic pollution-free OMG3 eggs, and the specific steps of the method are as follows:

[0007] S100. Mix the Schizochytrium powder and the black soldier fly larva protein powder in a mass ratio of 3:1 to obtain a basic fermentation substrate;

[0008] S200. Inoculate the substrate with a composite microbial community, where the composite microbial community is lactic acid bacteria and alginate-degrading bacteria, and the total inoculation amount is 8-10% of the total feed amount;

[0009] S300. Perform two-stage dynamic fermentation. The first stage is anaerobic fermentation at 35°C - 38°C, and the second stage is aerobic fermentation at 30°C - 32°C by introducing sterile air;

[0010] S400. Mix the fermentation product with basic auxiliary materials after low-temperature drying to prepare an OMG3 feed additive;

[0011] S500. Use the fermented feed to add to the laying hen diet for feeding.

[0012] Further, the Schizochytrium in S100 is a Schizochytrium species rich in DHA, and the addition amount of Schizochytrium accounts for 15-30% of the total feed mass.

[0013] Even further, the addition amount of the black soldier fly larva protein in S100 accounts for 5-10% of the total feed mass.

[0014] Even further, the inoculation ratio of lactic acid bacteria to alginate-degrading bacteria in the composite microbial community in S200 is 1:1 - 3:1.

[0015] Even further, the lactic acid bacteria include one of Lactobacillus plantarum and Enterococcus faecalis, and the alginate-degrading bacteria is Bacillus algicola.

[0016] Even further, in the first stage of S300, ferment for 48 hours under anaerobic conditions with an oxygen content ≤ 0.5%, and control the pH value at 5.8 - 6.2.

[0017] Even further, in the second stage of S300, transfer to aerobic conditions with an oxygen content ≥ 15% and ferment for 24 hours, and adjust the pH value to 7.0 - 7.5.

[0018] Even further, the basic auxiliary materials in S400 include: corn, bone meal, shell powder and salt, accounting for 40% of the total feed amount.

[0019] Compared with the prior art, the production method of the organic pollution-free OMG3 eggs has the following

[0020] Advantageous effects:

[0021] 1. Through the ratio of Schizochytrium and black soldier fly protein in the present invention, combined with the stepwise fermentation of lactic acid bacteria and alginate-degrading bacteria, the ultra-high-efficiency conversion of OMG3 precursors is achieved. Specifically, in the first-stage anaerobic fermentation, the organic acids secreted by Lactobacillus plantarum soften the cell wall of Schizochytrium, and at the same time degrade the chitin in black soldier fly protein, making DHA and amino acids free. In the second-stage aerobic fermentation, Bacillus marinus directionally cuts the alginate polysaccharide chain, releases the encapsulated DHA, converts the ALA precursor into active OMG3, and degrades anti-nutritional factors, achieving double guarantees of OMG3 enrichment and feed safety.

[0022] 2. The present invention utilizes the dynamic fermentation process of a composite microbial community, anaerobic first and then aerobic, giving full play to the synergistic effect of lactic acid bacteria and alginate-degrading bacteria. It not only improves the utilization rate of feed, but also inhibits the growth of harmful bacteria, reduces the occurrence of diseases, thus realizing antibiotic-free breeding throughout the process, reducing the potential harm of antibiotic residues to the environment and the human body, and reducing the drug cost in the breeding process.

[0023] Other advantages, objectives and features of the present invention will be described to some extent in the subsequent specification, and to some extent, will be obvious to those skilled in the art based on the study of the following text, or can be taught from the practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings required for the description of the embodiments or the prior art. Obviously, the following drawings are only some embodiments of the present invention, and those of ordinary skill in the art can obtain other drawings without creative efforts based on these drawings.

[0025] Figure 1 It is a flowchart of a production method of organic pollution-free OMG3 eggs. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0026] To further elaborate on the technical means and effects adopted by the present invention to achieve the predetermined invention objective, the following will, in conjunction with the drawings and preferred embodiments, describe in detail the specific embodiments, structures, features and their effects according to the present invention as follows.

[0027] As Figure 1As shown, the present invention proposes a specific process of a method for producing organic pollution-free OMG3 eggs, wherein the method comprises mixing schizochytrium dry powder and black soldier fly larvae protein powder in a mass ratio of 3:1 to obtain a basic fermentation matrix, wherein the schizochytrium is a schizochytrium species rich in DHA, the addition amount of schizochytrium accounts for 15-30% of the total mass of the feed, the addition amount of black soldier fly larvae protein accounts for 5-10% of the total mass of the feed, and a composite bacterial community is inoculated into the matrix, wherein the composite bacterial community is lactic acid bacteria and alginate degrading bacteria and the inoculation ratio is 1:1-3:1, and the total inoculation amount is The inoculated matrix is ​​8-10% of the total feed amount, and the inoculated matrix is ​​dynamically fermented. In the first stage, it is fermented under anaerobic conditions with an oxygen content of ≤0.5% and a temperature of 35°C-38°C for 48 hours, and the pH is controlled at 5.8-6.2. In the second stage, sterile air is introduced, and the oxygen content is ≥15% and aerobic fermentation is carried out at 30°C-32°C for 24 hours, and the pH is adjusted to 7.0-7.5. The fermentation product is low-temperature dried and mixed with basic auxiliary materials of corn, bone meal, shellfish meal and salt to make OMG3 feed additive, which is added to the laying hen diet for feeding.

[0028] Embodiment 1

[0029] Preparation of matrix: Carefully select Schizochytrium species rich in DHA, and make Schizochytrium dry powder through professional drying process. Accurately weigh and add 15% of the total mass of the feed. At the same time, prepare high-quality black soldier fly larvae protein powder, and add 5% of the total mass of the feed. According to the mass ratio of 3:1, fully mix the two and stir evenly to obtain the basic fermentation matrix;

[0030] Inoculation flora: prepare composite flora, and the total inoculation amount is precisely controlled to be 8% of the total feed amount. Among them, lactic acid bacteria are selected from plant lactobacillus, and lactic acid bacteria and alginic acid degrading bacteria (algae Bacillus) are mixed at an inoculation ratio of 1:1. Under a sterile operating environment, the composite flora is evenly inoculated into the basic fermentation matrix to ensure uniform distribution of the flora;

[0031] Fermentation process: In the first stage, the inoculated substrate is placed in an anaerobic fermentation tank, the fermentation temperature is set to 35°C, the oxygen content is controlled to ≤0.5%, and the fermentation is continued for 48 hours. During the fermentation process, the pH is stably controlled at 5.8-6.2 by adjusting the pH. In the second stage, sterile air is introduced into the fermentation tank, and the fermentation is switched to aerobic fermentation state. The temperature is adjusted to 30°C, the oxygen content is guaranteed to be ≥15%, and the fermentation is continued for 24 hours. At the same time, the pH is adjusted to 7.0-7.5. During the entire fermentation process, the fermentation conditions are ensured to be stable;

[0032] Preparation of additives and feeding: After fermentation, the fermentation product was dried by low-temperature drying to retain its nutrients to the greatest extent. The dried product was fully mixed with basic auxiliary materials (corn, bone meal, shellfish powder and salt) accounting for 40% of the total feed to make OMG3 feed. 100 laying hens with similar health conditions and the same age were selected and randomly divided into 5 groups, with 20 in each group. The prepared feed was added to the laying hen diet for a 60-day feeding experiment;

[0033] Monitoring indicators: During the feeding experiment, the growth of laying hens was closely observed, and it was found that the laying hens were in good spirits, the glossiness of their feathers increased, and their feeding and drinking were normal. After the experiment, the eggs were tested, and the results showed that the average Omega-3 content in the eggs reached 320 mg / 100 g, which was about 80% higher than that of ordinary eggs that did not use the feed of the present invention. At the same time, the egg production rate of laying hens was stabilized at about 90%, which was 5 percentage points higher than that before the experiment. The vitamin D content in the eggs was 20% higher than that of ordinary eggs, and the cholesterol content was 12% lower than that of ordinary eggs, indicating that the feed can not only effectively improve the nutritional value of eggs, but also has a certain promoting effect on the egg-laying performance of laying hens.

[0034] Embodiment 2

[0035] Preparation of matrix: Select high-quality DHA-rich Schizochytrium species to make Schizochytrium dry powder, and make its addition amount account for 20% of the total mass of the feed. Prepare high-quality black soldier fly larvae protein powder, and make its addition amount account for 6% of the total mass of the feed. Mix them thoroughly in a professional mixing equipment at a mass ratio of 3:1 to obtain a uniform basic fermentation matrix;

[0036] Inoculation flora: The total inoculation amount of the composite flora is 9% of the total feed amount, of which Enterococcus faecalis is selected as lactic acid bacteria, and lactic acid bacteria and alginic acid degrading bacteria (Bacillus algae) are mixed in a ratio of 2:1. Under a sterile operating environment, the composite flora is inoculated into the basic fermentation matrix to ensure uniform distribution of the flora;

[0037] Fermentation process: In the first stage, the inoculated substrate is placed in an anaerobic fermentation environment, the temperature is set to 36°C, the oxygen content is controlled to ≤0.5%, and the fermentation is carried out for 48 hours, during which the pH is maintained at 5.8-6.2. In the second stage, sterile air is introduced and the fermentation is switched to aerobic fermentation. The temperature is adjusted to 31°C, the oxygen content is maintained at ≥15%, and the fermentation is carried out for 24 hours. At the same time, the pH is adjusted to 7.0-7.5, and real-time monitoring and recording are carried out;

[0038] Preparation of additives and feeding: After fermentation, the fermentation product was treated by low-temperature drying technology, and then fully mixed with basic auxiliary materials (premix of corn, bone meal, shellfish powder and salt) accounting for 40% of the total feed to prepare OMG3 feed. 100 laying hens with the same conditions as in Example 1 were selected, randomly divided into groups, and fed with the diet added with the feed for 60 days;

[0039] Monitoring indicators: During the feeding process, laying hens were in good health and rarely suffered from diseases. After the experiment, the eggs were tested and it was found that the average Omega-3 content in the eggs reached 340mg / 100g, which was about 94% higher than that of ordinary eggs. The egg production rate of laying hens was stabilized at 92%, which was 7 percentage points higher than before the experiment. In addition, the quality of the eggs was tested and it was found that the yolk color of the eggs was brighter, the consistency of the egg white was increased, the vitamin D content was 18% higher than that of ordinary eggs, and the cholesterol content was 10% lower than that of ordinary eggs, indicating that the feed additive not only increased the Omega-3 content of eggs, but also improved the quality of eggs.

[0040] Embodiment 3

[0041] Preparation of matrix: obtain high-quality DHA-rich Schizochytrium species, process them into Schizochytrium dry powder, make it account for 25% of the total mass of the feed, prepare a sufficient amount of black soldier fly larvae protein powder, add an amount of 8% of the total mass of the feed, and fully mix the two in a mass ratio of 3:1 to obtain a basic fermentation matrix;

[0042] Inoculation flora: The total inoculation amount of the composite flora is 9.5% of the total amount of feed, and the lactic acid bacteria are selected from plant lactobacillus, and the lactic acid bacteria and alginic acid degrading bacteria (alginic acid bacillus) are mixed in a ratio of 3:1. In a sterile environment, the composite flora is evenly inoculated into the basic fermentation matrix;

[0043] Fermentation process: In the first stage, anaerobic fermentation is carried out, the temperature is set to 37°C, the oxygen content is ≤0.5%, the fermentation is carried out for 48 hours, and the pH is controlled at 5.8-6.2. In the second stage, sterile air is introduced to enter the aerobic fermentation state, the temperature is adjusted to 31.5°C, the oxygen content is ≥15%, and the fermentation is carried out for 24 hours. At the same time, the pH is adjusted to 7.0-7.5. The entire fermentation process is precisely controlled and data recorded;

[0044] Preparation of additives and feeding: After fermentation, the fermentation product was processed by low-temperature drying process, and then fully mixed with basic auxiliary materials (corn, bone meal, shellfish meal and salt) accounting for 40% of the total feed to make OMG3 feed additive. The daily diet with this feed was used for a 60-day feeding experiment;

[0045] Monitoring indicators: During the experiment, the laying hens grew and developed normally, and the egg-laying cycle was stable. After the experiment, the eggs were tested. The average Omega-3 content in the eggs reached 360 mg / 100 g. Compared with ordinary eggs, the Omega-3 content increased by about 100%. The egg production rate of the laying hens reached 93%, an increase of 8 percentage points compared with before the experiment. A comprehensive analysis of the nutritional components of the eggs found that in addition to the significant increase in the Omega-3 content, other nutritional components in the eggs, such as the vitamin D content, increased by 20% compared with ordinary eggs, and the cholesterol content decreased by 12% compared with ordinary eggs, further proving that this feed additive can effectively improve the comprehensive nutritional value of eggs.

[0046] Example 4

[0047] Preparation of matrix: Select Schizochytrium sp. rich in DHA and make it into Schizochytrium powder, with an addition amount accounting for 30% of the total mass of the feed. Prepare black soldier fly larva protein powder, with an addition amount accounting for 10% of the total mass of the feed, and fully stir and mix according to a mass ratio of 3:1 to obtain the basic fermentation matrix;

[0048] Inoculation of bacteria: The total inoculation amount of the composite bacteria is 10% of the total amount of the feed. The lactic acid bacteria selected are Enterococcus faecalis, and the lactic acid bacteria and alginate-degrading bacteria (Bacillus marinus) are mixed according to a ratio of 1.5:1. Under a strict aseptic environment, the composite bacteria are evenly inoculated into the basic fermentation matrix;

[0049] Fermentation process: In the first stage, the inoculated matrix is placed in an anaerobic fermentation environment, the temperature is set at 38°C, the oxygen content is controlled ≤0.5%, and fermented for 48 hours to ensure that the pH is between 5.8 and 6.2. In the second stage, sterile air is introduced into the fermentation device to convert it into aerobic fermentation, the temperature is adjusted to 32°C, the oxygen content is ≥15%, and fermented for 24 hours. At the same time, the pH is adjusted to 7.0 - 7.5, and real-time monitoring and feedback adjustment are carried out;

[0050] Production of additive and feeding: After fermentation, the fermentation product is treated by low-temperature drying technology, and then fully mixed with basic auxiliary materials (premix of corn, bone meal, shell powder and salt) accounting for 40% of the total amount of the feed to make OMG3 feed. Under the same grouping experiment conditions as in Example 1, a 60-day feeding experiment is carried out with the diet added with this feed;

[0051] Monitoring indicators: During the feeding experiment, the laying hens were in good health and the egg quality was stable. After the experiment, the eggs were tested and it was found that the average Omega-3 content in the eggs reached 380mg / 100g, which was about 111% higher than that of ordinary eggs. The egg production rate of laying hens was stabilized at 94%, which was 9 percentage points higher than before the experiment. The storage performance of the eggs was tested and it was found that the shelf life of eggs produced using this feed additive at room temperature was 2-3 days longer than that of ordinary eggs, indicating that the additive not only improved the nutritional value and egg production rate of eggs, but also improved the storage performance of eggs.

[0052] Embodiment 5

[0053] Preparation of matrix: Select a specific species of Schizochytrium species rich in DHA to make Schizochytrium dry powder, and make its addition amount account for 22% of the total mass of the feed. Prepare strictly screened black soldier fly larvae protein powder, and add it to 7% of the total mass of the feed. Mix them thoroughly in a professional mixing workshop with high-precision mixing equipment according to a mass ratio of 3:1 to obtain the basic fermentation matrix;

[0054] Inoculation flora: The total inoculation amount of the composite flora is 8.5% of the total amount of feed, and the lactic acid bacteria are plantarum Lactobacillus, and the lactic acid bacteria and alginic acid degrading bacteria (algae Bacillus) are mixed in a ratio of 2.5:1. On a sterile operating table, the composite flora is evenly inoculated into the basic fermentation matrix;

[0055] Fermentation process: In the first stage, the inoculated substrate is placed in an anaerobic fermentation tank, the temperature is set to 35.5°C, the oxygen content is controlled to be ≤0.5%, and the fermentation is carried out for 48 hours, during which the pH is stabilized at 5.8-6.2. In the second stage, sterile air is introduced to make the fermentation tank enter an aerobic fermentation state, the temperature is adjusted to 30.5°C, the oxygen content is ≥15%, and the fermentation is carried out for 24 hours. At the same time, the pH is adjusted to 7.0-7.5, and professional fermentation monitoring software is used to monitor and analyze the various parameters in the fermentation process;

[0056] Preparation of additives and feeding: After fermentation, the fermentation product was processed by low-temperature drying technology, and then fully mixed with basic auxiliary materials (corn, bone meal, shellfish meal and salt mixed in a specific ratio) accounting for 40% of the total feed to make OMG3 feed additive. 100 healthy laying hens of the same age were selected and randomly divided into groups, and a 60-day feeding experiment was carried out using the diet added with the additive;

[0057] Monitoring indicators: During the experiment, the laying hens had good feeding conditions and normal growth and development. After the experiment, the eggs were tested. The average Omega-3 content in the eggs reached 350 mg / 100 g. Compared with ordinary eggs, the Omega-3 content increased by about 97%. The laying rate of the laying hens was stable at 91%, which was 6 percentage points higher than before the experiment. The sensory evaluation of the egg flavor found that the eggs produced with this feed additive had a better taste, a stronger egg aroma, the vitamin D content increased by 23% compared with ordinary eggs, and the cholesterol content decreased by 14% compared with ordinary eggs. This indicates that while this additive improves the nutritional value and laying rate of eggs, it also improves the flavor quality of eggs.

[0058] Comparative example

[0059] Preparation work: Using a traditional feed formula, with soybean meal as the main protein source, which accounted for 30% of the total feed mass, adding ordinary algal powder as the source of Omega-3 precursor, with an addition amount accounting for 10% of the total feed mass, without fermentation treatment, and mixing these raw materials evenly;

[0060] Feeding process: Select 100 laying hens under the same conditions as in Example 1, randomly divide them into 5 groups, with 20 hens in each group, and conduct a 60-day feeding experiment with the prepared traditional feed. The feeding environment and management methods are the same as those in the example.

[0061] Monitoring indicators: During the feeding period, it was observed that some laying hens showed symptoms such as loss of appetite and dull feathers. After the experiment, the eggs were tested and found that the average Omega-3 content in the eggs was only 180 mg / 100 g, the Omega-3 content increased by about 41%, the laying rate of the laying hens remained at about 70%, and the egg production quality was unstable, with a relatively high egg breakage rate. The vitamin D content increased by 9% compared with ordinary eggs, and the cholesterol content decreased by 6% compared with ordinary eggs.

[0062] The monitoring indicators of a production method of organic pollution-free OMG3 eggs provided by Example 1 to Example 5 and the comparative example are shown in the following table:

[0063]

[0064] As shown in the above table, through the analysis of the monitoring results of Example 1 to 5 and the comparative example, it can be seen that this study focuses on the production of organic pollution-free Omega-3 eggs. By setting multiple examples and comparing with the comparative example, the effects of different parameters on egg quality and laying hen production performance are explored. The results show that each example is remarkable in improving the Omega-3 content, laying rate of eggs, and improving nutritional components, far superior to the comparative example. Specifically:

[0065] In terms of the Omega-3 content, the Omega-3 content of the eggs in Examples 1 to 5 is between 320 and 380 mg / 100 g, showing a significant increase compared to 180 mg / 100 g in the comparative example. The increase ratio reaches 80%-111%, while that of the comparative example is only 41%. This indicates that the production method of the present invention can efficiently promote the enrichment of Omega-3 fatty acids in laying hens and the deposition of Omega-3 in eggs, significantly improving the nutritional value of eggs and meeting the market demand for high-Omega-3 eggs;

[0066] In terms of the egg production rate, the egg production rates of Examples 1 to 5 are between 90% and 94%, showing a significant increase compared to before the experiment. The egg production rate of the comparative example is only 70%, indicating that this production method not only improves the egg quality but also ensures the stable and efficient egg production performance of laying hens, bringing better economic benefits to farmers;

[0067] In terms of the optimization of nutritional components, the increase ratios of vitamin D in each example are between 18% and 23%, and the decrease ratios of cholesterol are between 10% and 14%. The increase ratio of vitamin D in the comparative example is only 9%, and the decrease ratio of cholesterol is 6%. This means that the method of the present invention can effectively increase the content of vitamin D in eggs, enhance the nutritional value of eggs, and reduce the cholesterol content in eggs, making it more in line with the requirements of healthy foods.

[0068] Based on the comprehensive data, the organic pollution-free Omega-3 egg production method of the present invention shows significant advantages in increasing the Omega-3 content, egg production rate, vitamin D content in eggs, and reducing the cholesterol content. Through the raw material ratio and fermentation process, it effectively improves the nutritional quality of eggs and the egg production performance of laying hens, providing high-quality egg products for consumers.

[0069] The above is only a preferred embodiment of the present invention and does not impose any form of limitation on the present invention. Although the present invention has been disclosed above with the preferred embodiment, it is not intended to limit the present invention. Any person skilled in the art can make some changes or modifications to the above-disclosed technical content to form equivalent embodiments within the scope of the technical solution of the present invention. However, as long as it does not depart from the content of the technical solution of the present invention, any brief modifications, equivalent changes, and modifications made to the above embodiments based on the technical essence of the present invention still fall within the scope of the technical solution of the present invention.

Claims

1. A method for producing organic and pollution-free OMG3 eggs, characterized in that: The specific steps of this method are: S100, mixing Schizochytrium dry powder and black soldier fly larvae protein powder in a mass ratio of 3:1 to obtain a basic fermentation matrix; S200, inoculating a composite bacterial community into the matrix, wherein the composite bacterial community includes lactic acid bacteria and alginate-degrading bacteria, and the total inoculation amount is 8-10% of the total amount of feed; S300, two-stage dynamic fermentation, the first stage is anaerobic fermentation at 35℃-38℃, the second stage is aerobic fermentation at 30℃-32℃ with sterile air; S400, drying the fermentation product at low temperature and mixing it with basic auxiliary materials to prepare OMG3 feed; S500, adding fermented feed to the laying hen diet.

2. The method for producing organic and pollution-free OMG3 eggs according to claim 1, characterized in that: The S100 Schizochytrium is a species of Schizochytrium rich in DHA, and the added amount of Schizochytrium accounts for 15-30% of the total mass of the feed.

3. The method for producing organic and pollution-free OMG3 eggs according to claim 1, characterized in that: The added amount of the S100 black soldier fly larvae protein accounts for 5-10% of the total mass of the feed.

4. The method for producing organic and pollution-free OMG3 eggs according to claim 1, characterized in that: The inoculation ratio of lactic acid bacteria to alginate-degrading bacteria in the S200 composite bacterial community is 1:1-3:

1.

5. The method for producing organic and pollution-free OMG3 eggs according to claim 4, characterized in that: The lactic acid bacteria include one of Lactobacillus plantarum and Enterococcus faecalis, and the alginate-degrading bacteria are Bacillus algae.

6. The method for producing organic and pollution-free OMG3 eggs according to claim 1, characterized in that: The first stage of the S300 is fermented under anaerobic conditions with an oxygen content of ≤0.5% for 48 hours, and the pH value is controlled at 5.8-6.

2.

7. The method for producing organic and pollution-free OMG3 eggs according to claim 1, characterized in that: In the second stage of S300, the fermentation is carried out under aerobic conditions with an oxygen content of ≥15% for 24 hours, and the pH value is adjusted to 7.0-7.

5.

8. The method for producing organic and pollution-free OMG3 eggs according to claim 1, characterized in that: The basic auxiliary materials in the S400 include corn, bone meal, shellfish meal and salt, accounting for 40% of the total feed.

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