Preparation method of high-activity vaginal probiotic pellets
Highly active vaginal probiotic pellets are prepared through spraying rounding technology and multi-layer coating technology, which solves the problem of difficult preservation of probiotic activity in traditional preparations, and achieves improved activity and controlled release during production and shelf life.
Patent Information
- Application Number
- CN202510288349.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-03-12
- Publication Date
- 2025-06-03
AI Technical Summary
Traditional vaginal probiotic preparations are difficult to effectively protect probiotic activity, resulting in reduced or lost activity during production, storage and shelf life, affecting their efficacy.
Highly active vaginal probiotic pellets are prepared by spraying rounding technology, and probiotic activity is protected through multi-layer coating technology, including the pill core layer, the first layer, the second layer and the protective layer of the active ingredient, combined with low-temperature drying method to maximize the prevalence of probiotic activity.
It effectively improves the activity of probiotics during production and shelf life, achieves the controlled release of active ingredients, avoids suppression and high-temperature damage to bacterial species, and is suitable for transportation and storage at room temperature.
Smart Images
Figure CN120078646A_ABST
Abstract
Description
Technical Field
[0001] The present invention relates to the field of highly active vaginal probiotics, and particularly to a method for preparing pellet pills of highly active vaginal probiotics. Background Art
[0002] Vaginal microecological balance is crucial for female reproductive health. Probiotics play a key role in maintaining vaginal microecological balance. However, the preservation of probiotic activity has always been a difficult problem. Traditional preparation methods are often difficult to effectively protect the activity of probiotics, and it is easy to cause a decrease in activity or complete loss of activity during production, storage, and shelf life, thus affecting the exertion of their efficacy. Therefore, it is necessary to develop a method for preparing pellet pills of highly active vaginal probiotics that can effectively improve the production and preservation of probiotic activity. Summary of the Invention
[0003] The purpose of the present invention is to solve the problems in the background art, and provide a method for preparing pellet pills of highly active vaginal probiotics to improve the activity of probiotics during production and shelf life preservation, and achieve controlled release of active ingredients.
[0004] The above technical purpose of the present invention is achieved through the following technical solutions: A method for preparing pellet pills of highly active vaginal probiotics includes the following steps: S1, extrusion and spheronization: Weigh a certain weight of isomaltulose according to the core layer formula, mix it evenly, add an appropriate amount of purified water to make a soft material, and obtain the core mother nucleus layer through extrusion and spheronization. Pre-dry it at a temperature of 30 - 45 °C for 2 - 2.5 hours; S2, first spraying and spheronization: Turn on the air blower, mix the prebiotic and postbiotic powders, and spray and spheronize them outside the core mother nucleus layer through a high-efficiency coating machine to complete the preparation of the core composite layer; S3, second spraying and spheronization: Mix the bacterial powders of Lactobacillus crispatus and Lactobacillus gasseri in a certain proportion, and then spray and spheronize them outside the core composite layer through a high-efficiency coating machine to complete the preparation of the first layer of active ingredients; S3, third spraying and spheronization: Mix the bacterial powders of Lactobacillus rhamnosus, Lactobacillus mucosae, and Lactiplantibacillus plantarum evenly in a certain proportion, and then spray and spheronize them outside the first layer of active ingredients through a high-efficiency coating machine to complete the preparation of the second layer of active ingredients; S4, fourth spraying and spheronization: Mix arabic gum and fucoidan oligosaccharide to make a coating solution, and spray it outside the second layer of active ingredients through a high-efficiency coating machine, controlling the temperature below 30 - 45 degrees to complete the preparation of the active ingredient protection layer; S5, fifth spraying and spheronization: Dissolve chitosan oligosaccharide and microcrystalline cellulose to make a coating solution, and coat the pellet pills with the active ingredient layer through a high-efficiency coating machine to form a sustained-release protection layer; S6. Low-temperature drying: Place the wrapped pellet pills in a high-efficiency coating machine, adjust the temperature to 30 - 40°C, and dry for 3 - 5 hours to obtain pellet pills of highly active vaginal probiotics.
[0005] In the present invention, Lactobacillus crispatus and Lactobacillus gasseri powder are sprayed onto the pill cores to form the first layer of active ingredients. Then, the powder of Lactobacillus rhamnosus, Lactobacillus mucosae, and Lactobacillus plantarum is sprayed and rounded in the same way to obtain the second layer of active ingredients. This spraying process is superior to the traditional tableting process, which can avoid the pressing and high-temperature damage to the bacterial strains, and protect the probiotic activity to the greatest extent at the production end. After that, a coating layer is used to protect the probiotic active layer to achieve heat insulation and water isolation, which can better guarantee the activity of probiotics during the shelf life. At the same time, it is convenient for transportation and storage at room temperature, thereby improving the activity of probiotics during production and shelf life preservation and realizing the controlled release of active ingredients.
[0006] Comprehensively guarantee the probiotic activity from aspects such as production environment equipment, production formula, and process: Ultra-low temperature production environment and customized equipment: The entire production environment and process flow of the vaginal probiotic pellet pills are operated with the temperature controlled below 40°C. And customized equipment is adopted, which can reduce the loss of probiotics in the high-temperature environment to the greatest extent.
[0007] A coating layer is used to protect the probiotic active layer to achieve heat insulation and water isolation, which can better guarantee the activity of probiotics during the shelf life. At the same time, it is convenient for transportation and storage at room temperature.
[0008] The ultra-low temperature drying method can sublimate the moisture in probiotics at low temperature, and retain the activity and biological characteristics of probiotics to the greatest extent.
[0009] Preferably, the high-efficiency coating machine includes a box body, a coating drum, a hot air pipeline, an exhaust pipeline, and an adjustable discharge amount spraying mechanism. The coating drum is rotatably arranged in the box body, and a stirring paddle is arranged inside the coating drum. This is a mature existing technology, so it will not be elaborated in this case. A sealable door that can be opened is arranged on the side wall of the box body at the feeding port of the coating drum. The adjustable discharge amount spraying mechanism is arranged on the inner wall of the sealable door. The hot air pipeline is arranged above the right side of the coating drum and extends outside the box body. The exhaust pipeline is arranged below the left side of the coating drum and extends outside the box body. The coating drum can be driven to rotate through a pulley, a belt, and a motor. This is a very mature technology, so it will not be elaborated in this case.
[0010] In the present invention, the material is placed in a rotatable coating drum for rotation. The temperature inside the drum can be controlled through a hot air duct and an exhaust duct. The adjustable discharge amount spraying mechanism enables the coating drum to be applicable to different powders and liquids, and can precisely control the spraying amount, air flow rate, and material movement, ensuring a uniform thickness of the coating layer, avoiding problems such as uneven coating or local over-thickness / under-thickness, guaranteeing the consistency and stability of the product, and meeting high-quality standards.
[0011] Preferably, the adjustable discharge amount spraying mechanism includes a fixed rod fixedly connected to the sealing door, spray guns with adjustable discharge amounts, a powder fluidization box, and a coating liquid storage box arrayed on the fixed rod. The spray guns are provided with a high-pressure gas inlet and a low-pressure inlet. The powder fluidization box is connected to the low-pressure inlet, and the coating liquid storage box is connected to the low-pressure inlet. The coating liquid storage box and the powder fluidization box are controlled by a valve. When the powder fluidization box inputs to the low-pressure inlet, the coating liquid storage box is closed by the valve. When the coating liquid storage box is in use, the powder fluidization box is closed by the valve.
[0012] The spray gun includes a gun body. Inside the gun body, there are a needle valve core, a needle valve core control mechanism, and a nozzle. The gun body is divided into a high-pressure gas introduction area, an atomization area, and a secondary atomization area from left to right. One end of the nozzle is arranged in the high-pressure gas introduction area, and the other end extends into the atomization area. The nozzle is provided with a nozzle flow channel. The needle valve core is slidably installed in the nozzle flow channel. A high-pressure gas inlet is opened on the side wall of the gun body in the high-pressure gas introduction area, and the high-pressure gas inlet communicates with the nozzle flow channel. A low-pressure inlet is opened on the side wall of the gun body in the atomization area, and the needle valve core control mechanism controls the sliding of the needle valve core in the nozzle flow channel.
[0013] In the present invention, the mainstream compressed gas is conveyed to the nozzle flow channel through the high-pressure gas inlet. The needle valve core control mechanism controls the precise movement of the needle valve core on the nozzle flow channel according to requirements, controls the opening degree of the needle valve core, thereby realizing the continuous change of the variable flow path diameter of the nozzle flow channel, controlling the flow rate, enabling the material entering from the low-pressure inlet to be entrained, and the aspirated fluidized powder to be mixed with the high-speed air flow to achieve injection atomization. It can be applicable to different powders and liquids according to requirements, and can precisely control the spraying amount and air flow rate.
[0014] Preferably, the needle valve core control mechanism includes a rotating gear, a passive rack, a potentiometer, and a rotating motor. The rotating gear is fixedly arranged on the side wall of the starting end of the needle valve core. The passive rack is arranged along the length direction of the needle valve core. The rotating gear meshes with the passive rack. The output end of the rotating motor is fixedly connected to the middle of the rotating gear. The motor end of the rotating motor is fixed on the side wall of the gun body. The knob of the potentiometer is fixed in the middle of the rotating gear. The potentiometer is fixed on the gun body. The rotating motor is a speed reduction motor capable of positive and negative rotation, and the speed reduction motor is equipped with a built-in reduction gear, which can slow down the rotation speed of the rotating motor to ensure that the controller has sufficient time to receive the feedback information of the potentiometer, thereby improving the control accuracy and precision. The end of the nozzle flow channel is a variable cross-section flow channel with a gradually decreasing diameter, and the end of the needle valve core cooperates with the variable cross-section flow channel.
[0015] In the present invention, the controller controls the rotation of the rotating motor, and the motor controls the rotation of the driving gear, thereby controlling the left and right movement of the driving rack, driving the left and right movement of the needle valve core, and realizing the continuous change of the outlet diameter of the variable cross-section flow channel. The rotation of the driving gear drives the change of the potentiometer knob. The potentiometer transmits the rotation change of the gear to the controller to feedback the telescopic stroke of the needle valve core, thereby achieving the automatic and precise control of the movement of the needle valve core.
[0016] Preferably, the secondary atomization zone includes a secondary gasification main pipe and secondary gasification branch pipes. An auxiliary gas inlet is provided on the side wall of the secondary gasification main pipe. The auxiliary gas is compressed gas. The secondary gasification main pipe is fixed at the end of the gun body. The secondary gasification branch pipes are circumferentially and arrayedly distributed on the side wall of the secondary gasification main pipe and are in communication with each other.
[0017] In the present invention, compressed gas is introduced through the auxiliary gas inlet, so that the material atomized in the atomization zone is atomized again through the secondary gasification branch pipes, reducing the uneven state generated by the linear spray pressure gasification in the high-pressure gas introduction zone, thereby achieving uniform atomization and ensuring uniform thickness of the coating layer.
[0018] Preferably, a gas equalizing mechanism is provided between each secondary gasification branch pipe and the secondary gasification main pipe. The gas equalizing mechanism includes a hollow sleeve, a conical valve core, a compression spring, and a sealing ring. One end of the sleeve is connected to the secondary gasification main pipe, and the other end is connected to the secondary gasification branch pipe. The conical valve core is arranged in the sleeve, and the tip of the conical valve core faces the secondary gasification main pipe. A retaining ring is provided on the side wall of the sleeve near the secondary gasification branch pipe. The compression spring is arranged between the conical valve core and the retaining ring. An annular groove is provided on the side wall of the large diameter end of the conical valve core, and a sealing ring is arranged in the annular groove. The elastic force of the compression spring pressing against the conical valve core in the secondary gasification branch pipe closer to the secondary gasification main pipe is greater. Hollow The present invention controls the position of the conical valve core in the sleeve by the force of the compression spring pressing against the conical valve core, so as to control the amount of compressed gas passing through. The elastic forces of the compression springs of each gas equalizing mechanism are different, so that the gas passing through each secondary gasification branch pipe is the same, thereby ensuring the uniformity of secondary atomization.
[0019] Preferably, the needle valve core is horizontal with the nozzle flow channel and is located in the middle of the nozzle flow channel to enhance the uniformity of atomization.
[0020] Preferably, there are more than three balance blocks on the side wall of the needle valve core. The balance blocks include a fixing ring fixedly connected to the side wall of the needle valve core. Limiting grooves are formed on the side walls of the fixing ring. Ball bearings are embedded in the limiting grooves. The ball bearings can roll in the limiting grooves. More than three limiting grooves are formed along the circumferential direction of the fixing ring.
[0021] Through the design of the balance blocks, the present invention enables the needle valve core to always maintain balance, preventing the needle valve core from tilting and hitting the side wall of the nozzle flow channel during the movement process.
[0022] Preferably, the hot air duct and the exhaust duct are detachable structures and can be fixed by screws. Doors that can be opened are provided on the left and right sides of the box body. A cleaning frame is provided at the bottom of the coating drum. An electric push rod is provided at the bottom of the cleaning frame. The push rod end of the electric push rod is fixedly connected to the cleaning frame. The motor end of the electric push rod is fixed inside the box body. The water in the cleaning frame is discharged by a water pump.
[0023] In the present invention, the hot air duct and the exhaust duct are removed through the doors on the left and right sides. Then, a cleaning solution is added to the cleaning frame. Then, the electric push rod pushes the cleaning frame towards the coating drum. Then, the coating drum rotates slowly for immersion cleaning.
[0024] In summary, the beneficial effects of the present invention are as follows: 1. In the present invention, the powder of Lactobacillus crispatus and Lactobacillus gasseri is sprayed onto the pill cores to form the first layer of active ingredients. Then, the powders of Lactobacillus rhamnosus, Lactobacillus mucosae, and Lactobacillus plantarum are sprayed and rounded in the same way to obtain the second layer of active ingredients. This spraying process is superior to the traditional tableting process, which can avoid the pressing and high-temperature damage to the bacterial strains, protect the activity of probiotics to the greatest extent at the production end. Then, a coating layer is used to protect the probiotic active layer to achieve heat insulation and water isolation, which can better guarantee the activity of probiotics during the shelf life and is convenient for transportation and storage at room temperature. Thus, the activity of probiotics during production and shelf life preservation is improved, and the controlled release of active ingredients is realized; 2. The present invention rotates the material placed in the drum by a rotatable coating drum, controls the temperature inside the drum through a hot air duct and an exhaust duct, and enables the coating drum to be applicable to the spraying of different materials such as the powder of Lactobacillus rhamnosus, Lactobacillus mucosae, Lactobacillus plantarum, the powder of Lactobacillus rhamnosus, Lactobacillus mucosae, Lactobacillus plantarum, and coating liquid through an adjustable discharge amount spraying mechanism. This allows the encapsulation of the pellet to be carried out only in one coating machine, reducing movement and the pollution problems brought during the movement process, enhancing the product quality. At the same time, it can accurately control the spray amount, air flow rate, and material movement, ensuring a uniform thickness of the coating layer and avoiding problems such as uneven coating or local over-thick / over-thin, guaranteeing the consistency and stability of the product and meeting high-quality standards. 3. The present invention transports the mainstream compressed gas to the nozzle flow channel through a high-pressure gas inlet. The spray needle valve core control mechanism controls the precise movement of the needle valve core on the nozzle flow channel according to requirements, controls the opening degree of the needle valve core, thereby realizing the continuous change of the variable flow path diameter of the nozzle flow channel, controlling the flow rate, enabling the material entering from the low-pressure inlet to be entrained, and mixing the aspirated fluidized powder with the high-speed air flow to achieve ejector atomization. It can be applicable to different powders and liquids according to requirements and can accurately control the spray amount and air flow rate. 4. The present invention introduces compressed gas through an auxiliary gas inlet, enabling the material atomized in the atomization zone to be atomized again through a secondary gasification branch pipe, reducing the uneven state generated by the linear spray pressure gasification in the high-pressure gas introduction zone, thereby achieving uniform atomization and ensuring a uniform thickness of the coating layer. BRIEF DESCRIPTION OF THE DRAWINGS
[0025] Figure 1 is a schematic flow diagram of the adjustable discharge amount spraying mechanism of the present invention; Figure 2 is a schematic connection diagram of the rotating motor, driving gear, and potentiometer of the present invention; Figure 3 is a schematic diagram of the balance weight of the present invention; Figure 4 is a cross-sectional schematic diagram of the gas equalizing mechanism of the present invention; Figure 5 is a schematic diagram of the conical valve core of the gas equalizing mechanism of the present invention being pushed; Figure 6 is a three-dimensional schematic diagram of the coating machine of the present invention; Figure 7 is a schematic diagram of the cleaning frame of the present invention; Figure 8 is a schematic diagram of the adjustable discharge amount spraying mechanism inside the coating drum of the present invention.
[0026] Description of the drawings: The high-efficiency coating machine; 11. Box body; 12. Coating drum; 13. Hot air pipeline; 14. Exhaust pipeline; 15. Adjustable discharge amount spraying mechanism; 121. Sealing door; 151. Fixed rod; 16. Spray gun; 152. Powder fluidization box; 153. Coating liquid storage box; 161. High-pressure gas inlet; 162. Low-pressure powder inlet; 163. Gun body; 164. Needle valve core; 17. Needle valve core control mechanism; 166. Nozzle; 167. High-pressure gas introduction area; 168. Atomization area; 169. Secondary atomization area; 160. Nozzle flow channel; 171. Rotating gear; 172. Passive rack; 173. Potentiometer; 174. Rotating motor; 175. Secondary gasification main pipe; 176. Secondary gasification branch pipe; 177. Auxiliary gas inlet; 2. Gas equalizing mechanism; 21. Internally hollow sleeve; 22. Conical valve core; 23. Compression spring; 24. Sealing ring; 20. Balance weight; 201. Fixed ring; 202. Limit groove; 204. Ball; 122. Cleaning frame; 123. Electric push rod. Detailed implementation manners
[0027] The following specific embodiments are only explanations of the present invention, and they do not limit the present invention. Those skilled in the art can make modifications without creative contributions to this embodiment according to needs after reading this specification, but as long as they are within the scope of the claims of the present invention, they are protected by the patent law.
[0028] The present invention will be described in detail below with reference to the accompanying drawings by way of examples.
[0029] Example 1
[0030] A method for preparing granule pills of highly active vaginal probiotics includes the following steps: S1. Extrusion and spheronization: Weigh a certain weight of isomaltulose according to the formula of the core layer, mix it evenly, add an appropriate amount of purified water to make a soft material, and obtain the core mother nucleus layer through extrusion and spheronization. Pre-dry it at 35°C for 2 hours. S2. First spraying and spheronization: Turn on the air blast, mix the prebiotics and postbiotics powders, and spray and spheronize them on the outside of the core mother nucleus layer through the high-efficiency coating machine 1 to complete the preparation of the core composite layer. S3. Second spraying and spheronization: Mix the bacterial powders of Lactobacillus crispatus and Lactobacillus gasseri in a certain proportion, and then spray and spheronize them on the outside of the core composite layer through the high-efficiency coating machine 1 to complete the preparation of the first layer of active ingredients. S3. Third spraying and spheronization: Mix the bacterial powders of Lactobacillus rhamnosus, Lactobacillus mucosae, and Lactiplantibacillus plantarum evenly in a certain proportion, and then spray and spheronize them on the outside of the first layer of active ingredients through the high-efficiency coating machine 1 to complete the preparation of the second layer of active ingredients. S4. Fourth spraying and rounding: Mix gum arabic and fucoidan oligosaccharide to prepare a coating solution, and spray it on the outside of the second layer of active ingredients through the high-efficiency coating machine 1, with the temperature controlled below 35°C to complete the preparation of the active ingredient protection layer; S5. Fifth spraying and rounding: Dissolve chitosan oligosaccharide and microcrystalline cellulose to prepare a coating solution, and coat the particle pills already wrapped with the active ingredient layer through the high-efficiency coating machine 1 to form a sustained-release protection layer; S6. Low-temperature drying: Place the wrapped particle pills in the high-efficiency coating machine 1, adjust the temperature to 35°C, and dry for 4 hours to obtain the particle pills of highly active vaginal probiotics.
[0031] As Figure 6 shown, the high-efficiency coating machine 1 includes a box body 11, a coating drum 12, a hot air duct 13, an exhaust duct 14, and an adjustable discharge amount spraying mechanism 15. The coating drum 12 is rotatably arranged in the box body 11. A sealable door 121 is provided on the side wall of the box body 11 at the feeding port of the coating drum 12. The adjustable discharge amount spraying mechanism 15 is arranged on the inner wall of the sealable door 121. The hot air duct 13 is arranged above the right side of the coating drum 12 and extends outside the box body 11. The exhaust duct 14 is arranged below the left side of the coating drum 12 and extends outside the box body 11. A rotating pipe is connected to the outside of the sealable door, and the rotating pipe is fixedly connected to the box body through a bearing, so as to ensure that the sealed box body can be rotated out of the coating drum.
[0032] As Figures 1-3As shown, the adjustable discharge amount spraying mechanism 15 includes a fixed rod 151 fixedly connected to the sealing door 121, spray guns 16 with adjustable discharge amounts, a powder fluidization tank 152, and a coating liquid storage tank 153 arrayed on the fixed rod 151. The spray gun 16 is provided with a high-pressure gas inlet 161 and a low-pressure inlet 162. The powder fluidization tank 152 is connected to the low-pressure inlet 162, and the coating liquid storage tank 153 is connected to the low-pressure inlet 162. The spray gun 16 includes a gun body 163. A needle valve core 164, a needle valve core control mechanism 17, and a nozzle 166 are arranged in the gun body 163. The gun body 163 is divided into a high-pressure gas introduction area 167, an atomization area 168, and a secondary atomization area 169 from left to right. One end of the nozzle 166 is arranged in the high-pressure gas introduction area 167, and the other end extends into the atomization area 168. A nozzle flow channel 160 is arranged in the nozzle 166. The needle valve core 164 is slidably installed in the nozzle flow channel 160. A high-pressure gas inlet 161 is arranged on the side wall of the gun body 163 in the high-pressure gas introduction area 167, and the high-pressure gas inlet 161 communicates with the nozzle flow channel 160. A low-pressure inlet 162 is arranged on the side wall of the gun body 163 in the atomization area 168. The needle valve core control mechanism 17 controls the sliding of the needle valve core 164 in the nozzle flow channel 160. The needle valve core control mechanism 17 includes a rotating gear 171, a passive rack 172, a potentiometer 173, and a rotating motor 174. The rotating gear 171 is fixedly arranged on the side wall of the starting end of the needle valve core 164. The passive rack 172 is arranged along the length direction of the needle valve core 164. The rotating gear 171 meshes with the passive rack 172. The output end of the rotating motor 174 is fixedly connected to the middle of the rotating gear 171. The motor end of the rotating motor 174 is fixed on the side wall of the gun body 163. The knob of the potentiometer 173 is fixed to the middle of the rotating gear 171, and the potentiometer is fixed on the gun body 161. The secondary atomization area 169 includes a secondary gasification main pipe 175 and secondary gasification branch pipes 176. An auxiliary gas inlet 177 is arranged on the side wall of the secondary gasification main pipe 175. The auxiliary gas is compressed gas. The secondary gasification main pipe 175 is fixed to the end of the gun body 163. The secondary gasification branch pipes 176 are circumferentially and arrayedly distributed on the side wall of the secondary gasification main pipe 175 and are in communication with each other.
[0033] As Figures 4-5As shown, a gas distribution mechanism 2 is provided between each secondary gasification branch pipe 176 and the secondary gasification main pipe 175. The gas distribution mechanism 2 includes a hollow sleeve 21, a conical valve core 22, a compression spring 23, and a sealing ring 24. One end of the sleeve 21 is connected to the secondary gasification main pipe 175, and the other end is connected to the secondary gasification branch pipe 176. The conical valve core 22 is arranged inside the sleeve 21, and the tip of the conical valve core 22 faces the secondary gasification main pipe 175. A retaining ring 25 is provided on the side wall of the sleeve 21 near the end of the secondary gasification branch pipe 176. The compression spring 23 is arranged between the conical valve core 22 and the retaining ring 25. An annular groove 221 is provided on the side wall of the large-diameter end of the conical valve core 22, and the sealing ring 24 is arranged in the annular groove. The elastic force of the compression spring pressing against the conical valve core in the secondary gasification branch pipe closer to the secondary gasification main pipe is greater. The needle valve core 164 is horizontal with the nozzle flow channel 160 and is located in the middle of the nozzle flow channel 160. More than three balance blocks 20 are provided on the side wall of the needle valve core 164. The balance blocks 20 include a fixing ring 201 fixedly connected to the side wall of the needle valve core 164. Limiting grooves 202 are formed on the side walls of the fixing ring 201. The limiting grooves 202 are embedded with balls 204, and the balls 204 can roll in the limiting grooves 202. More than three limiting grooves 202 are formed along the circumferential direction of the fixing ring 201.
[0034] As Figure 7 shown, the hot air pipe 13 and the exhaust pipe 14 are detachable structures. Doors that can be opened are provided on the left and right sides of the box body 11. A cleaning frame 122 is provided at the bottom of the coating drum 12. An electric push rod 123 is provided at the bottom of the cleaning frame 122. The push rod end of the electric push rod 123 is fixedly connected to the cleaning frame 122, and the motor end of the electric push rod 123 is fixed inside the box body 11. The water in the cleaning frame is discharged by a water pump.
[0035] Working principle: As Figures 1-8As shown in the figure, when the high-efficiency coating machine 1 is in use, the core mother nucleus layer is poured into the coating drum 12. The coating drum 12 rotates. At the same time, the prebiotic and postbiotic powders are mixed and fluidized in the powder fluidization box 152. According to the controller, the rotation of the rotation motor 174 is controlled. The rotation motor 174 controls the rotation of the driving gear, thereby controlling the left and right movement of the driving rack, driving the left and right movement of the needle valve core 164, realizing the continuous change of the outlet diameter of the variable flow channel. The rotation of the driving gear drives the change of the potentiometer knob. The potentiometer transmits the rotation change of the gear to the controller, feeding back the telescopic stroke of the needle valve core 164, so that the spray gun 16 with adjustable discharge amount adjusts the position of the needle valve core 164 from the nozzle head according to different materials. Then, the high-pressure gas inlet 161 conveys the mainstream compressed gas to the nozzle flow channel 160, so that the fluidized powder is entrained and mixed with the high-speed air flow to achieve jet atomization. Then, compressed gas is introduced through the auxiliary gas inlet, so that the material atomized in the atomization zone is atomized again through the secondary gasification branch pipe 175 to achieve uniform atomization. It rotates while spraying, so that the prebiotic and postbiotic powders are sprayed and rounded outside the core mother nucleus layer, completing the preparation of the core composite layer. Then, the bacterial powders of Lactobacillus crispatus and Lactobacillus gasseri are sprayed and rounded outside the core composite layer in the same way, completing the preparation of the first layer of active ingredients. The bacterial powders of Lactobacillus rhamnosus, Lactobacillus mucosae, and Lactobacillus plantarum are sprayed and rounded outside the first layer of active ingredients to complete the preparation of the second layer of active ingredients. Then, the powder fluidization box 152 is closed by a valve, and the channel between the coating liquid storage box 153 and the spray gun is opened. The coating liquid made by mixing arabic gum and fucoidan oligosaccharide is sprayed outside the second layer of active ingredients to complete the preparation of the active ingredient protective layer. Finally, chitosan oligosaccharide and microcrystalline cellulose are dissolved to make a coating liquid, and the high-efficiency coating machine 1 is used to coat the granular pills with the active ingredient layer wrapped, forming a sustained-release protective layer. Then, the temperature is adjusted to 30-40°C and dried for 3-5 hours to obtain the granular pills of high-activity vaginal probiotics.
[0036] Example 2
[0037] Different from Example 1, a method for preparing granular pills of high-activity vaginal probiotics includes the following steps: S1. Extrusion and spheronization: Weigh a certain weight of isomaltulose according to the core layer formula, mix it evenly, add an appropriate amount of purified water to make a soft material, and obtain the core mother nucleus layer through extrusion and spheronization. At a temperature of 30°C, pre-dry for 2 hours; S2. First spraying and spheronization: Turn on the air blower, mix the prebiotic and postbiotic powders, and spray and spheronize them outside the core mother nucleus layer through the high-efficiency coating machine 1 to complete the preparation of the core composite layer; S3. Second spraying and spheronization: Mix the bacterial powders of Lactobacillus crispatus and Lactobacillus gasseri in a certain proportion, and then spray and spheronize them outside the core composite layer through the high-efficiency coating machine 1 to complete the preparation of the first layer of active ingredients; S3. Three - time spraying and rolling: Mix the powder of Lactobacillus rhamnosus, Lactobacillus mucosae, and Lactiplantibacillus plantarum evenly in a certain proportion, and then spray and roll it on the outside of the first layer of active ingredients through the high - efficiency coating machine 1 to complete the preparation of the second layer of active ingredients; S4. Four - time spraying and rolling: Mix arabic gum and fucoidan oligosaccharide to make a coating solution, spray it on the outside of the second layer of active ingredients through the high - efficiency coating machine 1, and control the temperature below 35 °C to complete the preparation of the protective layer of active ingredients; S5. Five - time spraying and rolling: Dissolve chitosan oligosaccharide and microcrystalline cellulose to make a coating solution, and coat the granules with the active ingredient layer through the high - efficiency coating machine 1 to form a sustained - release protective layer; S6. Low - temperature drying: Place the coated granules in the high - efficiency coating machine 1, adjust the temperature to 30 °C, and dry for 4 hours to obtain the granules of high - activity vaginal probiotics.
[0038] Example 3
[0039] Different from Example 1, A method for preparing granules of high - activity vaginal probiotics, comprising the following steps: S1. Extrusion and rolling: Weigh a certain amount of isomaltulose according to the formula of the core layer, mix it evenly, add an appropriate amount of purified water to make a soft material, and obtain the core mother - nucleus layer through extrusion and rolling. Pre - dry at 45 °C for 2 hours; S2. First - time spraying and rolling: Turn on the air blower, mix prebiotics and postbiotics powder, and spray and roll it on the outside of the core mother - nucleus layer through the high - efficiency coating machine 1 to complete the preparation of the core composite layer; S3. Second - time spraying and rolling: Mix the powder of Lactobacillus crispatus and Lactobacillus gasseri in a certain proportion, and then spray and roll it on the outside of the core composite layer through the high - efficiency coating machine 1 to complete the preparation of the first layer of active ingredients; S3. Three - time spraying and rolling: Mix the powder of Lactobacillus rhamnosus, Lactobacillus mucosae, and Lactiplantibacillus plantarum evenly in a certain proportion, and then spray and roll it on the outside of the first layer of active ingredients through the high - efficiency coating machine 1 to complete the preparation of the second layer of active ingredients; S4. Four - time spraying and rolling: Mix arabic gum and fucoidan oligosaccharide to make a coating solution, spray it on the outside of the second layer of active ingredients through the high - efficiency coating machine 1, and control the temperature below 35 °C to complete the preparation of the protective layer of active ingredients; S5. Five - time spraying and rolling: Dissolve chitosan oligosaccharide and microcrystalline cellulose to make a coating solution, and coat the granules with the active ingredient layer through the high - efficiency coating machine 1 to form a sustained - release protective layer; S6. Low - temperature drying: Place the coated granules in the high - efficiency coating machine 1, adjust the temperature to 40 °C, and dry for 4 hours to obtain the granules of high - activity vaginal probiotics.
[0040] Comparative Example 1 A method for preparing granular pills of highly active vaginal probiotics comprises the following steps: S1, extrusion and spheronization, according to the pellet core layer formula, a certain weight of isomaltulose is weighed and mixed evenly, and then an appropriate amount of purified water is added to make a soft material, and the pellet core mother core layer is obtained by extrusion and spheronization, and pre-dried at 45°C for 2 hours; S2, spraying and spheronizing once, turning on the air blowing, mixing the prebiotic and postbiotic powders, and spraying and spheronizing them on the outside of the core layer of the pellet core through the high-efficiency coating machine 1, and completing the preparation of the composite layer of the pellet core; S3, secondary spraying and spheronization, mixing the powders of Lactobacillus crispatus and Lactobacillus gasseri in a certain proportion, and then spraying and spheronizing on the outside of the composite layer of the pill core through a high-efficiency coating machine 1 to complete the preparation of the first layer of active ingredients; S3, spraying and spheronizing three times, mixing the powders of Lactobacillus rhamnosus, Lactobacillus reuteri and Lactobacillus plantarum in a certain proportion, and then spraying and spheronizing through a high-efficiency coating machine 1 to complete the preparation of the second layer of active ingredients outside the first layer of active ingredients.
[0041] With reference to the method of GB / T 4789.2-2016 "Determination of the total number of colonies in food microbiology", the granular pellets prepared in Example 1, Example 2, Example 3, and Comparative Example 1 were placed in a natural environment for 7 days, and then one granular pellet prepared in Example 1, Example 2, Example 3, and Comparative Example 1 was taken for serial dilution with sterile saline, shaken or stirred until the contents were completely released, 20 μL was aspirated and spotted on MRS culture medium and placed for 20 minutes, and then placed in a 37°C incubator for 48 hours, and the number of colonies formed on the plate was counted. The results are listed in the table below.
[0042]
[0043] It can be seen from the above results that the preparation method of Example 1 of the present invention can effectively improve the activity and shelf life of probiotics. In a simulated vaginal environment, probiotics can be released at an expected sustained-release rate, have a significant inhibitory effect on common vaginal pathogens, and have good application prospects.
Claims
1. A method for preparing granular pills of highly active vaginal probiotics, characterized in that: The following steps are involved: S1, extrusion and spheronization, according to the pellet core layer formula, a certain weight of isomaltulose is weighed and mixed evenly, and then an appropriate amount of purified water is added to make a soft material, and the pellet core mother core layer is obtained by extrusion and spheronization, and pre-dried at a temperature of 30-45°C for 2-2.5 hours; S2, spraying and spheronizing once, turning on the air blowing, mixing the prebiotic and postbiotic powders, and spraying and spheronizing them on the outside of the pellet core layer through a high-efficiency coating machine (1), thereby completing the preparation of the pellet core composite layer; S3, secondary spraying and spheronization, mixing the powders of Lactobacillus crispatus and Lactobacillus gasseri in a certain proportion, and then spraying and spheronizing them on the outside of the composite layer of the pill core through a high-efficiency coating machine (1), thereby completing the preparation of the first layer of active ingredients; S3, spraying and spheronizing three times, mixing the powders of Lactobacillus rhamnosus, Lactobacillus mucilaginosus, and Lactobacillus plantarum in a certain proportion, and then spraying and spheronizing the powders of the active ingredient outside the first layer of the active ingredient through a high-efficiency coating machine (1) to complete the preparation of the second layer of the active ingredient; S4, spraying and spheronizing for four times, mixing gum arabic and brown algae oligosaccharide to form a coating liquid, spraying it on the outside of the second layer of the active ingredient through a high-efficiency coating machine (1), and controlling the temperature below 30-45 degrees to complete the preparation of the active ingredient protective layer; S5, spraying and spheronizing for five times to dissolve chitosan oligosaccharide and microcrystalline cellulose to prepare a coating solution, and coating the granules coated with the active ingredient layer by a high-efficiency coating machine (1) to form a sustained-release protective layer; S6, low temperature drying, placing the coated granules in a high-efficiency coating machine (1), adjusting the temperature to 30-40°C, and drying for 3-5 hours to obtain granules containing highly active vaginal probiotics.
2. The method for preparing a highly active vaginal probiotic granule pill according to claim 1, characterized in that: The high-efficiency coating machine (1) comprises a housing (11), a coating drum (12), a hot air duct (13), an exhaust duct (14), and an adjustable discharge amount spraying mechanism (15); the coating drum (12) is rotatably arranged in the housing (11); an openable sealing door (121) is provided on the side wall of the housing (11) at the feeding port of the coating drum (12); the adjustable discharge amount spraying mechanism (15) is arranged on the inner wall of the sealing door (121); the hot air duct (13) is arranged on the upper right side of the coating drum (12) and extends to the outside of the housing (11); and the exhaust duct (14) is arranged on the lower left side of the coating drum (12) and extends to the outside of the housing (11).
3. The method for preparing a highly active vaginal probiotic granule pill according to claim 2, characterized in that: The adjustable discharge spraying mechanism (15) comprises a fixed rod (151) fixedly connected to the sealing door (121), a spray gun (16) with an adjustable discharge arranged on the fixed rod (151), a powder fluidizing box (152), and a coating liquid storage box (153); the spray gun (16) is provided with a high-pressure gas inlet (161) and a low-pressure inlet (162); the powder fluidizing box (152) is connected to the low-pressure inlet (162); and the coating liquid storage box (153) is connected to the low-pressure inlet (162).
4. The method for preparing a highly active vaginal probiotic granule pill according to claim 3, characterized in that: The spray gun (16) comprises a gun body (163), wherein a needle valve core (164), a needle valve core control mechanism (17), and a nozzle (166) are arranged in the gun body (163), wherein the gun body (163) is divided into a high-pressure gas introduction area (167), an atomization area (168), and a secondary atomization area (169) from left to right, wherein one end of the nozzle (166) is arranged in the high-pressure gas introduction area (167), and the other end extends into the atomization area (168), wherein a nozzle flow channel (160) is arranged in the nozzle (166), and wherein The needle valve core (164) is slidably installed in the nozzle flow channel (160); the high-pressure gas inlet (161) is opened on the side wall of the gun body (163) located in the high-pressure gas introduction area (167); the high-pressure gas inlet (161) is communicated with the nozzle flow channel (160); the low-pressure inlet (162) is opened on the side wall of the gun body (163) located in the atomization area (168); and the needle valve core control mechanism (17) controls the needle valve core (164) to slide in the nozzle flow channel (160).
5. The method for preparing a granular pill of highly active vaginal probiotics according to claim 4, characterized in that: The needle valve core control mechanism (17) comprises a rotating gear (171), a passive rack (172), a potentiometer (173), and a rotating motor (174); the rotating gear (171) is fixedly arranged on the side wall of the starting end of the needle valve core (164); the passive rack (172) is arranged along the length direction of the needle valve core (164); the rotating gear (171) and the passive rack (172) are meshed with each other; the output end of the rotating motor (174) is fixedly connected to the middle part of the rotating gear (171); the motor end of the rotating motor (174) is fixed on the side wall of the gun body (163); the knob of the potentiometer (173) is fixed on the middle part of the rotating gear (171); and the potentiometer (173) is fixed on the gun body (163).
6. The method for preparing a highly active vaginal probiotic granule pill according to claim 4, characterized in that: The secondary atomization zone (169) includes a secondary gasification main pipe (175) and a secondary gasification branch pipe (176). An auxiliary gas inlet (177) is provided on the side wall of the secondary gasification main pipe (175); the secondary gasification main pipe (175) is fixed to the end of the gun body (163); and the secondary gasification branch pipes (176) are distributed in a circular array on the side wall of the secondary gasification main pipe (175) and are in communication with the secondary gasification branch pipes (176).
7. The method for preparing a highly active vaginal probiotic granule pill according to claim 6, characterized in that: A gas homogenizing mechanism (2) is provided between each secondary gasification branch pipe (176) and the secondary gasification main pipe (175), the gas homogenizing mechanism (2) comprising a sleeve (21) with a hollow interior, a conical valve core (22), a compression spring (23), and a sealing ring (24); one end of the sleeve (21) is connected to the secondary gasification main pipe (175), and the other end is connected to the secondary gasification branch pipe (176); the conical valve core (22) is provided in the sleeve (21), and the tip of the conical valve core (22) faces the secondary gasification branch pipe (176). A gasification main pipe (175), a retaining ring (25) is provided on the side wall of one end of the sleeve (21) close to the secondary gasification branch pipe (176), the compression spring (23) is arranged between the conical valve core (22) and the retaining ring (25), an annular groove (221) is provided on the side wall of the large diameter end of the conical valve core (22), a sealing ring (24) is provided in the annular groove, and the closer to the secondary gasification main pipe, the greater the elastic force of the compression spring (23) in the secondary gasification branch pipe pressing against the conical valve core (22) is.
8. The method for preparing a highly active vaginal probiotic granule pill according to claim 4, characterized in that: The needle valve core (164) is kept horizontal with the nozzle flow channel (160) and is located in the middle of the nozzle flow channel (160).
9. The method for preparing a highly active vaginal probiotic granule pill according to claim 8, characterized in that: More than three balancing blocks (20) are provided on the side wall of the needle valve core (164), and the balancing block (20) includes a fixing ring (201) fixedly connected to the side wall of the needle valve core (164). A limiting groove (202) is provided on the side wall of the fixing ring (201), and a ball (204) is embedded in the limiting groove (202). The ball (204) can roll in the limiting groove (202), and more than three limiting grooves (202) are formed along the circumferential direction of the fixing ring (201).
10. The method for preparing a highly active vaginal probiotic granule pill according to claim 2, characterized in that: The hot air duct (13) and the exhaust duct (14) are detachable structures. The left and right sides of the box (11) are provided with doors that can be opened. The bottom of the coating drum (12) is provided with a cleaning frame (122). The bottom of the cleaning frame (122) is provided with an electric push rod (123). The push rod end of the electric push rod (123) is fixedly connected to the cleaning frame (122), and the motor end of the electric push rod (123) is fixed in the box (11).