Giant salamander liver peptide formula capable of protecting liver

Abstract

The invention belongs to the field of peptide preparation, and particularly relates to a liver-protecting giant salamander liver peptide formula which is characterized by comprising the following three proportioning modes: blending giant salamander liver peptide powder and alcohol according to the mass ratio of 0.1-10%; blending the giant salamander liver peptide enzymatic hydrolysate with alcohol according to a mass ratio of 1: 9 to 4: 1; carrying out enzymolysis on the giant salamander liver and mixed liquid of water and alcohol according to the mass ratio of 1: 9 to 9: 1, and blending; wherein the alcohol is alcohol with the alcohol concentration of 20%-90%, the giant salamander liver is fresh liver or cooked liver, the giant salamander liver and lead and other heavy metals in the human body form compounds and are discharged out of the body through the ability of metallothionein in the giant salamander liver to combine with metal and the high induction characteristic, liver protection and liver nourishing are achieved, and the health of a drinker is benefited; the giant salamander liver peptide is rich in various nutrient substances, can supplement elements required by a human body, can make the flavor of the wine more mellow, improves the consumer experience, and further improves the economic value of the product and the economic benefits of enterprises.

Description

Technical Field

[0001] The present invention belongs to the field of peptide preparation, and specifically relates to a hepatoprotective formula of giant salamander liver peptide. Background Art

[0002] The Chinese giant salamander belongs to the phylum Chordata and class Amphibia, and is currently a second-class wild protected animal in China. Thanks to the breakthrough of key technologies such as artificial reproduction and seedling cultivation, the breeding of giant salamanders has gradually become large-scale. The muscle protein of giant salamanders contains 18 kinds of amino acids, among which essential amino acids account for 44.08% of the total amino acids, making it an extremely high-quality source of animal protein. According to regulations, farmed giant salamanders can be reasonably developed and utilized as food resources.

[0003] However, at present, the giant salamander resources are facing a dilemma of oversupply. In terms of development and utilization, it is mainly limited to direct consumption or rough processing, and the in-depth development of giant salamander resources is extremely lacking. In particular, the development and research of active peptide products using giant salamander muscles are almost blank, which greatly restricts the further development of the giant salamander industry.

[0004] The existing giant salamander wine made from giant salamander muscles on the market, although it has some of the nutrition and flavor of giant salamanders, has many drawbacks. It does not have the function of protecting the liver and nourishing the liver. Excessive drinking is likely to make people drunk and has no promoting effect on the liver's detoxification ability. Moreover, the existing production process of giant salamander wine results in few active substances remaining in the wine, and the antioxidant activity is significantly low, with extremely limited effects in health care.

[0005] Therefore, the present invention provides a hepatoprotective formula of giant salamander liver peptide. Summary of the Invention

[0006] In order to make up for the deficiencies of the prior art and solve at least one technical problem proposed in the background art.

[0007] The technical solution adopted by the present invention to solve its technical problems is as follows: A hepatoprotective formula of giant salamander liver peptide according to the present invention is characterized in that it includes the following three mixing ratios:

[0008] The giant salamander liver peptide powder is mixed with alcohol at a mass ratio of 0.1% - 10%;

[0009] The giant salamander liver peptide enzymatic hydrolysate is mixed with alcohol at a mass ratio of 1:9 to 4:1;

[0010] The giant salamander liver is enzymatically hydrolyzed and mixed with a mixed liquid of water and alcohol at a mass ratio of 1:9 to 9:1;

[0011] Among them, the alcohol is alcohol with an alcohol concentration of 20% - 90%, and the giant salamander liver uses fresh liver or cooked liver.

[0012] A production process for a liver-protecting giant salamander liver peptide formula, characterized in that it is used to prepare a liver-protecting giant salamander liver peptide as described in claim 1, and the production process includes the following steps:

[0013] S1. Mash and enzymatically hydrolyze fresh giant salamander liver tissue to prepare a raw material for formulating liver peptide. During the enzymatic hydrolysis process, add the giant salamander liver tissue to an enzymatic hydrolysis solution containing water for enzymatic hydrolysis or add it to an enzymatic hydrolysis solution containing water and alcohol for enzymatic hydrolysis. The content of the complex enzyme in the enzymatic hydrolysis solution is 0.1% - 0.15%;

[0014] S2. Put the raw material obtained in step S1 into a centrifuge for centrifugal filtration to remove non-enzymatically hydrolyzable fibers and macromolecular substances and remove the grease therein;

[0015] S3. Mix the giant salamander liver peptide raw material after filtration in step S2 with alcohol according to the required ratio. If an enzymatic hydrolysis solution containing water and alcohol is used for enzymatic hydrolysis and the alcohol has reached the ratio condition, the step of preparing alcohol can be omitted;

[0016] S4. Put the mixed liquid in step S3 into a centrifuge again for filtration to remove the generated precipitate to obtain the finished product.

[0017] Preferably, the mashed giant salamander liver tissue in step S1 is subjected to steaming treatment and then put into an enzymatic hydrolysis solution for enzymatic hydrolysis.

[0018] Preferably, the giant salamander liver peptide enzymatic hydrolysis solution after enzymatic hydrolysis with an enzymatic hydrolysis solution containing only water in step S1 is subjected to drying treatment to prepare a giant salamander liver peptide powder for formulating liver peptide.

[0019] Preferably, the drying treatment method for the giant salamander liver peptide enzymatic hydrolysis solution adopts spray drying method.

[0020] Preferably, the drying treatment method for the giant salamander liver peptide enzymatic hydrolysis solution adopts freeze drying method.

[0021] Preferably, the liver tissue cell tissue fluid precipitated after steaming in step S1 is retained and added to the enzymatic hydrolysis solution after enzymatic hydrolysis or added to the mixed liquid in step S3.

[0022] The beneficial effects of the present invention are as follows:

[0023] 1. For the liver-protecting giant salamander liver peptide formula described in the present invention, through the metal-binding ability and high induction characteristics of metallothionein in the giant salamander liver, it forms compounds with heavy metals such as lead in the human body and excretes them out of the body, realizing liver protection and nourishment, which is beneficial to the health of drinkers; the giant salamander liver peptide is rich in various nutrients, can supplement the elements required by the human body, and can make the flavor of the wine more mellow, improving the consumer experience, and thus enhancing the economic value of the product and the economic benefits of the enterprise.

[0024] 2. The hepatoprotective giant salamander liver peptide formula of the present invention can be flexibly adapted to large - scale or small - scale production through various blending methods such as directly mixing the enzymatic hydrolysate with alcohol, mixing the freeze - dried liver peptide powder with the liquor, and mixing the spray - dried liver peptide powder with the liquor. It can also retain the active substances of the giant salamander liver peptide to the greatest extent, maintain the antioxidant activity of the product, and bring more benefits to the drinkers. At the same time, the precipitated tissue fluid is retained during the production process and added and mixed in the subsequent process, which not only helps the liver peptide dissolve in alcohol, reduces the waste of insoluble substances, but also improves the color of the final product, enhances the product appearance, and strengthens the comprehensive competitiveness of the product in the market. Detailed implementation manners

[0025] In order to make the technical means, creative features, achieved purposes and functions of the present invention easy to understand, the present invention will be further described below in conjunction with the specific implementation manners.

[0026] A hepatoprotective giant salamander liver peptide formula according to an embodiment of the present invention is characterized in that it includes the following three proportioning methods:

[0027] The giant salamander liver peptide powder and alcohol are blended at a mass ratio of 0.1% - 10%;

[0028] The giant salamander liver peptide enzymatic hydrolysate and alcohol are blended at a mass ratio of 1:9 to 4:1;

[0029] The giant salamander liver, water and alcohol mixed liquid are enzymatically hydrolyzed and blended at a mass ratio of 1:9 to 9:1;

[0030] Among them, the alcohol is alcohol with an alcohol concentration of 20% - 90%, and the giant salamander liver uses fresh liver or cooked liver.

[0031] A production process of a hepatoprotective giant salamander liver peptide formula is characterized in that it is used to prepare a hepatoprotective giant salamander liver peptide as described in claim 1, and this production process includes the following steps:

[0032] S1. Mash and enzymatically hydrolyze the fresh giant salamander liver tissue to prepare the giant salamander liver peptide raw material for blending. During the enzymatic hydrolysis process, the giant salamander liver tissue is added to the enzymatic hydrolysate containing water for enzymatic hydrolysis or added to the enzymatic hydrolysate containing water and alcohol for enzymatic hydrolysis, and the content of the complex enzyme in the enzymatic hydrolysate is 0.1% - 0.15%;

[0033] S2. Put the raw material obtained in step S1 into a centrifuge for centrifugal filtration to remove the non - enzymatically hydrolyzable fibers and macromolecular substances, and remove the grease therein;

[0034] S3. Mix the giant salamander liver peptide raw material filtered in step S2 with alcohol according to the required ratio. If the enzymatic hydrolysate containing water and alcohol is used for enzymatic hydrolysis and the alcohol therein has reached the ratio condition, the step of blending alcohol is omitted;

[0035] S4. Re-filter the mixed liquid obtained in step S3 in a centrifuge to remove the generated precipitate, thus obtaining the finished product.

[0036] Steam the mashed giant salamander liver tissue in step S1, and after steaming, place it in an enzyme solution for enzymatic hydrolysis.

[0037] Dry the giant salamander liver peptide hydrolysate obtained by enzymatic hydrolysis of the enzyme solution containing only water in step S1 to prepare giant salamander liver peptide powder for blending liver peptide.

[0038] The drying method for the giant salamander liver peptide hydrolysate is spray drying.

[0039] The drying method for the giant salamander liver peptide hydrolysate is freeze drying.

[0040] Retain the liver tissue cell tissue fluid precipitated after steaming in step S1, and add the tissue fluid to the enzyme solution after enzymatic hydrolysis or to the mixed liquid in step S3.

[0041] Example 1

[0042] In terms of raw material selection, use the liver tissue of artificially farmed giant salamanders;

[0043] First, according to the precise ratio of giant salamander liver to pure water of 1:1, put the liver into the steaming process. Steam it at a high temperature for 10 minutes. This process aims to fully cook the protein tissue in the liver. The cooked liver is quickly cooled to 50°C. This temperature range can not only avoid damage to the enzyme activity during the subsequent enzymatic hydrolysis due to excessive temperature but also maintain a suitable environment to facilitate the smooth progress of the next operation. Then, use a professional tissue smashing mixer to beat the cooled liver into a delicate and uniform minced meat shape. The purpose of this is to greatly increase the contact area between the liver and the subsequent added solution, creating favorable conditions for the efficient development of the enzymatic hydrolysis reaction.

[0044] Subsequently, add alcohol for solution preparation, and finally configure a solution system with an alcohol concentration of 60%. Under natural pH conditions, add a complex enzyme to this solution. The addition amount is strictly controlled at 2000 U / g, and the content of the complex enzyme in the whole system is precisely maintained at 0.1%. This precise enzyme addition amount and content setting are determined through a large number of previous experiments and research, aiming to ensure that the enzymatic hydrolysis reaction can proceed efficiently without being affected by too much or too little enzyme amount, thus affecting the quality and performance of the final product. After the enzyme addition is completed, place the solution in a water bath at 50°C and keep it warm for 4 hours while continuously stirring. The water bath can provide a stable and uniform temperature environment, and the stirring operation can ensure full contact between the enzyme and the liver minced meat, enabling the enzymatic hydrolysis reaction to proceed continuously under the best conditions.

[0045] After the 4-hour enzymatic hydrolysis reaction, heat inactivation treatment is carried out with boiling water (100 °C) for 15 minutes. High-temperature inactivation of enzymes can quickly terminate the enzymatic hydrolysis reaction, avoid excessive reaction and the generation of adverse by-products, thereby ensuring the stability of product quality. After the inactivation of enzymes is completed, the solution is cooled. After cooling to an appropriate temperature, the solution is transferred to a centrifuge and centrifuged at a speed of 5000 r / min for 10 minutes. Under the action of centrifugal force, the oil and other insoluble impurities in the solution quickly settle to the bottom of the centrifuge tube. At this time, carefully take the supernatant to obtain the preliminary enzymatic hydrolysate.

[0046] After obtaining the enzymatic hydrolysate, it is carefully filtered to further remove possible fine impurities. After the filtration is completed, the alcohol ratio contained in the enzymatic hydrolysate needs to be accurately detected. If the detection result shows that the alcohol ratio has not reached the target requirement, at this time, the enzymatic hydrolysate and alcohol need to be mixed and adjusted again to ensure that the alcohol concentration reaches the predetermined 50%. After the adjustment is completed, the mixed solution is filtered and precipitated again to remove the precipitate substances that may be generated during the adjustment process, and finally obtain the Andrias davidianus liver peptide wine product with an accurate alcohol concentration of 50%. If it is found during the detection that the alcohol ratio contained in the enzymatic hydrolysate has just reached the requirement, there is no need to perform the mixing and adjustment operation again, and the enzymatic hydrolysate can be directly filtered and precipitated to efficiently obtain the final product.

[0047] Example 2

[0048] Using the artificially cultured Andrias davidianus liver tissue as the starting material. First, cut the Andrias davidianus liver into small pieces with a size of 5×5 cm. Such a size of the cut pieces can not only ensure the convenience of subsequent crushing operations, but also prevent the liver from being overly fragmented during the crushing process, which may affect subsequent processing. Then, use a tissue homogenizer to beat the cut liver pieces into a delicate meat paste state to provide a larger action area for the subsequent enzymatic hydrolysis reaction.

[0049] Under natural pH conditions, add compound protease to the liver meat paste. The addition amount of the compound protease is set to 3000 U / g, and the content of the compound enzyme in the whole system is controlled at 0.15%. This specific addition amount and content setting are based on in-depth research on the protein structure and characteristics of the Andrias davidianus liver and a large number of experimental verifications, aiming to achieve the best enzymatic hydrolysis effect. After the addition is completed, let the enzymatic hydrolysis reaction continue for 4 hours. During these 4 hours, the compound protease fully acts on the proteins in the liver meat paste and gradually decomposes them into the required peptide substances.

[0050] After the enzymatic hydrolysis reaction is completed, heat inactivation treatment is carried out with boiling water (100 °C) for 15 minutes to quickly terminate the enzymatic hydrolysis reaction and ensure precise control of the reaction process. After the heat inactivation is completed, the reaction solution is cooled. After cooling to an appropriate temperature, the reaction solution is transferred to a centrifuge and centrifuged at a relatively high speed of 8000 r / min for 12 minutes. Compared with Example 1, higher speed and longer centrifugation time can more effectively remove the grease and other insoluble impurities in the reaction solution, so as to obtain a purer upper clear liquid, that is, the enzymatic hydrolysate.

[0051] The obtained enzymatic hydrolysate is subjected to freeze-drying treatment. Freeze-drying is a drying method that sublimates and removes moisture in a low-temperature environment. Its advantage is that it can retain the active substances in the enzymatic hydrolysate to the greatest extent and avoid the loss of active ingredients due to high-temperature drying. After freeze-drying, dried giant salamander liver peptide powder is obtained.

[0052] Next, the giant salamander liver peptide powder is mixed with the wine solution according to a mass ratio of 8%. During the mixing process, stir well to make the liver peptide powder evenly dispersed in the wine solution. At the same time, add the previously reserved liver tissue fluid for blending. The addition of the liver tissue fluid can not only further enrich the flavor and nutritional components of the wine solution, but also promote the dissolution of the liver peptide powder in the wine solution to a certain extent. After the mixing and blending are completed, the solution is filtered to remove the precipitated substances, and finally a giant salamander liver peptide wine product with a high content of active substances is obtained. Due to the retention of more active ingredients, this product may have more significant performance in protecting the liver and other beneficial effects on the human body.

[0053] The above shows and describes the basic principles, main features and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited by the above embodiments. What is described in the above embodiments and the specification only illustrates the principles of the present invention. Without departing from the spirit and scope of the present invention, the present invention will have various changes and improvements, and these changes and improvements fall within the scope of the present invention claimed. The scope of protection claimed by the present invention is defined by the appended claims and their equivalents.

Claims

1. A liver-protecting formula of giant salamander liver peptide, characterized in that: There are three matching methods: The giant salamander liver peptide powder and alcohol are mixed at a mass ratio of 0.1% to 10%; The giant salamander liver peptide enzymatic hydrolysate and alcohol are mixed at a mass ratio of 1:9 to 4:1; The liver of the giant salamander is enzymatically hydrolyzed and mixed with a mixed liquid of water and alcohol at a mass ratio of 1:9 to 9:1; The alcohols are all alcohols with an alcohol concentration of 20%-90%, and the giant salamander livers are fresh livers or steamed livers.

2. A production process of a liver-protecting giant salamander liver peptide formula, characterized in that: It is used to prepare the liver-protecting giant salamander liver peptide described in claim 1, and the production process comprises the following steps: S1. Crush and enzymolyze fresh liver tissue of giant salamander to prepare raw material of giant salamander liver peptide for adjusting liver peptide. In the enzymolysis process, add the liver tissue of giant salamander to enzymolysis solution containing water or to enzymolysis solution containing water and alcohol for enzymolysis, and the content of complex enzyme in the enzymolysis solution is 0.1% to 0.15%; S2, placing the raw material obtained in step S1 into a centrifuge for centrifugal filtration to remove fibers and macromolecular substances that cannot be enzymatically decomposed, and remove the grease therein; S3, mixing the giant salamander liver peptide raw material filtered in step S2 with alcohol according to a desired ratio. If an enzymolysis solution containing water and alcohol is used for enzymolysis, when the alcohol has reached the ratio condition, the step of preparing the alcohol is omitted; S4, putting the mixed liquid in step S3 into the centrifuge again for filtering, removing the generated precipitate, and obtaining a finished product.

3. The production process of a liver-protecting giant salamander liver peptide formula according to claim 2, characterized in that: The giant salamander liver tissue that has been crushed in step S1 is steamed, and then placed in an enzymatic hydrolysis solution for enzymatic hydrolysis.

4. The production process of a liver-protecting giant salamander liver peptide formula according to claim 2, characterized in that: The giant salamander liver peptide enzymatic hydrolyzate obtained by enzymatic hydrolyzation with the enzymatic hydrolyzate containing only water in step S1 is dried to prepare giant salamander liver peptide powder for preparing liver peptide.

5. The production process of a liver-protecting giant salamander liver peptide formula according to claim 2, characterized in that: The spray drying method is used to dry the enzymatic hydrolysate of giant salamander liver peptide.

6. The production process of a liver-protecting giant salamander liver peptide formula according to claim 2, characterized in that: The method for drying the giant salamander liver peptide hydrolysate is freeze-drying.

7. The production process of a liver-protecting giant salamander liver peptide formula according to claim 2, characterized in that: The liver tissue cell tissue fluid precipitated after the steaming in step S1 is retained, and the tissue fluid is added to the enzymatic hydrolyzed liquid after the enzymatic hydrolysis or added to the mixed liquid in step S3.