Bioactive material with anti-inflammatory and repairing functions as well as preparation method and application of bioactive material

Abstract

The invention discloses a bioactive material with anti-inflammatory and repairing functions as well as a preparation method and application of the bioactive material. The preparation method comprises the following steps: modifying silicon ions through amino siloxane to obtain aminated bioactive particles, and mixing the aminated bioactive particles with sodium hyaluronate, polyethylene glycol and cerium oxide particles to prepare the bioactive material with anti-inflammatory and repairing functions. By introducing the bioactive particles, the expression level of inflammation-related genes TNF-alpha and IL-6 can be remarkably inhibited, an excellent inflammation inhibition function is shown, proliferation and differentiation of cells can be promoted, and therefore rapid repair of damaged parts can be accelerated, and the treatment effect is good. The bioactive material can scavenge free radicals to inhibit excessive occurrence of inflammation at damaged tissue parts and further promote tissue regeneration and repair.

Description

Technical Field

[0001] The present invention belongs to the technical field of medical materials, and particularly relates to a bioactive material with both anti-inflammatory and repair functions, a preparation method thereof, and an application thereof in the preparation of products for promoting tissue repair and regeneration. Background Art

[0002] At present, the repair of damaged soft tissues, especially the skin soft tissues which are the largest organ of the human body, is a precise and complex process, and the inflammatory reaction is also an inevitable stage. Therefore, there is an urgent need to develop a functional material with anti-inflammatory effects and the ability to actively regulate endogenous factors to promote the healing of damaged areas. Bioactive nanoparticles have gradually become bioactive agents widely used in wound repair materials. Mesoporous silica particles have very promising application prospects as effective functional components of wound repair materials due to their unique hydrophilicity, biocompatibility, and ability to promote skin tissue repair. In addition, cerium dioxide is a type of rare earth oxide with a high surface area, which not only has good biocompatibility but also has certain anti-inflammatory ability by scavenging free radicals and reactive oxygen species. Moreover, sodium hyaluronate has the advantages of film-forming, lubricity, moisture retention, and breathability. Therefore, the preparation of a functional material containing anti-inflammatory components and bioactive repair components is of great significance for the repair of damaged tissues. Summary of the Invention

[0003] To solve the disadvantages and deficiencies of the prior art, the primary object of the present invention is to provide a preparation method of a bioactive material with both anti-inflammatory and repair functions.

[0004] Another object of the present invention is to provide a bioactive material with both anti-inflammatory and repair functions prepared by the above preparation method, which can scavenge free radicals to inhibit the excessive occurrence of inflammation at the damaged tissue site and further promote tissue regeneration and repair.

[0005] Another object of the present invention is to provide an application of the above bioactive material with both anti-inflammatory and repair functions in the preparation of products for promoting tissue repair and regeneration.

[0006] The object of the present invention is achieved by the following technical solutions:

[0007] A preparation method of a bioactive material with both anti-inflammatory and repair functions, comprising the following steps:

[0008] (1) Add cetyltrimethylammonium bromide to an ethanol / ammonia water mixed solution. After mixing evenly, add tetraethyl orthosilicate dropwise, then stir and react, centrifuge, wash, and dry to obtain bioactive particles;

[0009] (2) Add the bioactive particles to an amino silicone solution. After reaction, wash and dry to obtain amino-functionalized bioactive particles;

[0010] (3) Prepare sodium hyaluronate solution and polyethylene glycol solution separately, then mix the sodium hyaluronate solution and the polyethylene glycol solution evenly, and then add amino-functionalized bioactive particles and cerium oxide particles. After stirring and reacting, a bioactive material is obtained.

[0011] Preferably, the ratio of cetyltrimethylammonium bromide to tetraethyl orthosilicate in step (1) is 0.2 - 0.6 g : 1 - 2.5 mL; more preferably 0.4 - 0.5 g : 1.5 - 2 mL.

[0012] Preferably, the ethanol / ammonia water mixed solution in step (1) is obtained by stirring anhydrous ethanol, water and ammonia water at a volume ratio of (40 - 60) : (55 - 59.2) : (0.8 - 5) at 35 - 45 °C for 10 - 30 minutes; more preferably, the ethanol / ammonia water mixed solution is obtained by stirring anhydrous ethanol, water and ammonia water at a volume ratio of 45 : 53.2 : 1.8 at 40 °C for 20 minutes.

[0013] Preferably, the ratio of cetyltrimethylammonium bromide to the ethanol / ammonia water mixed solution in step (1) is 0.2 - 0.6 g : 500 mL; more preferably 0.4 - 0.5 g : 500 mL.

[0014] Preferably, the temperature of the stirring reaction in step (1) is 35 - 45 °C and the time is 2 - 6 hours; more preferably, the temperature of the stirring reaction is 40 °C and the time is 4 hours.

[0015] Preferably, the rotation speed of the centrifugation in step (1) is 3000 - 5000 revolutions per minute and the centrifugation time is 20 - 50 minutes.

[0016] Preferably, the washing in step (1) means washing 2 - 5 times with ethanol and water.

[0017] Preferably, the drying in step (1) means drying at 45 - 65 °C for 24 - 48 hours.

[0018] Preferably, the amino-siloxane in step (2) is at least one of 3-aminopropyltriethoxysilane, γ-aminopropyltriethoxysilane, γ-aminopropyltrimethoxysilane, N-β-aminoethyl-γ-aminopropyltrimethoxysilane, N-β-aminoethyl-γ-aminopropyltriethoxysilane and N,N-dimethylaminopropylaminopropylmethyldimethoxysilane.

[0019] Preferably, the concentration of the amino-siloxane solution in step (2) is 12 - 18 wt% and the solvent is n-hexane.

[0020] Preferably, the mass ratio of the bioactive particles to the aminosiloxane in step (2) is (0.5 - 2):(3 - 8).

[0021] Preferably, the temperature of the reaction in step (2) is 60 - 65°C and the time is 4 - 8 hours; more preferably, the temperature of the reaction is 60°C and the time is 5 hours.

[0022] Preferably, the washing in step (2) means washing with an aqueous solution of n - hexane.

[0023] Preferably, the drying in step (2) means drying at 45 - 65°C for 4 - 6 hours.

[0024] Preferably, the concentration of the sodium hyaluronate solution in step (3) is 0.1 - 10 g / L, more preferably 5 - 8 g / L.

[0025] Preferably, the concentration of the polyethylene glycol solution in step (3) is 10 - 80 g / L, more preferably 10 - 30 g / L.

[0026] Preferably, the volume ratio of the sodium hyaluronate solution to the polyethylene glycol solution in step (3) is 1:1.

[0027] Preferably, in step (3), the molecular weight range of the sodium hyaluronate is between 100 - 1000 kDa, more preferably 120 - 500 kDa; the molecular weight range of the polyethylene glycol is between 200 - 6000 Da, more preferably 2000 - 6000 Da.

[0028] Preferably, the mixing evenly in step (3) means stirring at room temperature for 30 - 60 minutes.

[0029] Preferably, in the bioactive material of step (3), the concentrations of the amino - functionalized bioactive particles and the cerium oxide particles are 20 - 100 g / L and 0.1 - 1 g / L respectively; more preferably 25 - 30 g / L and 0.25 - 0.3 g / L.

[0030] Preferably, the temperature of the stirring reaction in step (3) is room temperature and the time is 2 - 6 hours; more preferably 4 - 6 hours.

[0031] The present invention also provides a bioactive material with both anti - inflammatory and repair functions prepared by the above - mentioned preparation method.

[0032] The present invention also provides the application of the above - mentioned bioactive material with both anti - inflammatory and repair functions in the preparation of products for treating damaged tissue repair.

[0033] Compared with the prior art, the present invention has the following advantages and beneficial effects:

[0034] 1. Sodium hyaluronate and polyethylene glycol have good biocompatibility and are relatively safe for biomedical materials. Moreover, they increase the viscosity of the active material, prolong the action time of the material at the damaged site, and enhance the therapeutic effect of the active ingredients.

[0035] 2. The introduction of bioactive particles can significantly inhibit the expression levels of inflammation-related genes TNF-α and IL-6, showing excellent anti-inflammatory function.

[0036] 3. The introduction of bioactive particles can also promote cell proliferation and differentiation, thus accelerating the rapid repair of damaged sites. Description of the Drawings

[0037] Figure 1 It is a bar chart showing the promotion of cell proliferation by the bioactive material with both anti-inflammatory and repair functions prepared in Example 1 of the present invention. The blank group uses the well plate as a control.

[0038] Figure 2 It is the absorbance value of the bioactive material with both anti-inflammatory and repair functions prepared in Example 2 of the present invention for scavenging reactive oxygen species. DPPH (1,1-diphenyl-2-picrylhydrazyl radical) is a free radical widely used to evaluate antioxidant activity. Its ethanol solution is dark purple, has a strong absorption at 517 nm, and is relatively stable. When the lone pair electrons of the DPPH free radical are paired, the solution color becomes lighter and the absorbance value decreases. The change in the absorbance value is proportional to the ability of the bioactive material to scavenge free radicals. By measuring the change in the absorbance value, the scavenging rate of the bioactive material for DPPH free radicals can be calculated, thereby evaluating its ability to scavenge reactive oxygen species. At 517 nm, the absorbance of the model group (DPPH pure reagent group) in the figure is 0.903, while that of the experimental group (the material prepared in Example 2) is 0.266. It can be seen that the experimental group has excellent ability to scavenge reactive oxygen species.

[0039] Figure 3 It is a physical picture of the bioactive material with both anti-inflammatory and repair functions prepared in Example 2 of the present invention for scavenging reactive oxygen species ((a) is the model group, (b) is the experimental group, and from left to right in (b) are Comparative Example 1, Example 2, and Comparative Example 2). Without scavenging reactive oxygen species, it shows a deeper color. When it has the function of removing reactive oxygen species, the solution color turns light yellow. It can be seen that the experimental group has excellent ability to scavenge reactive oxygen species. Although Comparative Example 1 has excellent ability to scavenge reactive oxygen species, due to the excessive addition of cerium oxide, its cytotoxicity is relatively large; the ability of Comparative Example 2 to scavenge reactive oxygen species is significantly worse than that of Example 2.

[0040] Figure 4 It is a bar chart showing the inhibition of the expression of the inflammation-related gene TNF-α by the bioactive material with both anti-inflammatory and repair functions prepared in Example 2 of the present invention.

[0041] Figure 5Bar chart showing that the bioactive material with both anti-inflammatory and repair functions prepared in Example 2 of the present invention inhibits the expression of the inflammation-related gene IL-6.

[0042] Figure 6 Bar chart of the cytotoxicity of the bioactive materials with both anti-inflammatory and repair functions prepared in Example 2 and Comparative Example 1 of the present invention. Among them, the blank wells of the well plate serve as the blank group, Example 2 group, and Control Example 1 group. Detailed implementation manners

[0043] The present invention will be further described in detail below with reference to examples and drawings, but the implementation manners of the present invention are not limited thereto.

[0044] In the examples of the present invention, those not specified in detail are carried out according to conventional conditions or conditions recommended by the manufacturer. The raw materials, reagents, etc. without indicating the manufacturer can be obtained as conventional products through commercial purchase.

[0045] Example 1

[0046] This example provides a preparation method of a bioactive material with both anti-inflammatory and repair functions, including the following steps:

[0047] The first part: Preparation of bioactive particles

[0048] 1) Mix absolute ethanol, water, and ammonia water according to a volume ratio of 45:53.2:1.8, stir at 40 °C to obtain 500 mL of solution A, the stirring time is 20 minutes, and the concentration of ammonia water is 25-28 wt%.

[0049] 2) Weigh 0.4 g of cetyltrimethylammonium bromide and add it to solution A in 1) to obtain solution B, and stir for 20 minutes.

[0050] 3) Slowly add 1.5 mL of tetraethyl orthosilicate to solution B, and continuously stir at 40 °C for 4 hours during the addition process to obtain a milky white solution.

[0051] 4) Centrifuge the milky white solution in 3) to obtain a white material body, the centrifugation rate is 4000 revolutions per minute, and the centrifugation time is 30 minutes.

[0052] 5) Wash the white material body obtained in 4) alternately with absolute ethanol and water.

[0053] 6) Dry the final material body obtained in 5) in a vacuum drying oven at 60 °C for 32 hours to obtain bioactive particles.

[0054] 7) Add the bioactive particles into the n - hexane solution containing 15 wt% 3 - aminopropyltriethoxysilane at a mass - volume ratio of 3:100 g / mL, and stir - react at 60 °C for 5 hours.

[0055] 8) Add the modified bioactive particles obtained in 7) into a pure n - hexane solution, wash them twice repeatedly, and then dry them at 60 °C for 5 hours for later use.

[0056] The second part: Preparation of the bioactive material with both anti - inflammatory and repair functions

[0057] 1) Add 0.05 g of sodium hyaluronate with a molecular weight of 500 kDa into 100 mL of aqueous solution, and stir at 90 °C for 40 minutes to obtain solution A1.

[0058] 2) Add 3 g of polyethylene glycol with a molecular weight of 2000 Da into 100 mL of aqueous solution, and stir at 25 °C for 30 minutes to obtain solution B1.

[0059] 3) Mix solution A1 and solution B1 in a ratio of 1:1 to obtain solution C, and stir at room temperature for 60 minutes.

[0060] 4) Weigh 5 g of the modified bioactive particles and 0.05 g of cerium oxide particles respectively and add them into solution C, stir evenly at room temperature for 4 hours for a full reaction to obtain the bioactive material with both anti - inflammatory and repair functions.

[0061] Example 2

[0062] This example provides a preparation method of a bioactive material with both anti - inflammatory and repair functions, including the following steps:

[0063] The first part: Preparation of bioactive particles

[0064] 1) Mix anhydrous ethanol, water and ammonia water in a volume ratio of 45:53.2:1.8, stir at 40 °C to obtain 500 mL of solution A, with a stirring time of 20 minutes and the concentration of ammonia water being 25 - 28 wt%.

[0065] 2) Weigh 0.5 g of cetyltrimethylammonium bromide and add it into solution A in 1) to obtain solution B, and stir for 20 minutes.

[0066] 3) Slowly add 2 mL of tetraethyl orthosilicate into solution B, and continuously stir at 40 °C for 4 hours during the addition process to obtain a milky white solution.

[0067] 4) Centrifuge the milky white solution in 3) to obtain a white material body, with a centrifugation rate of 4500 revolutions per minute and a centrifugation time of 30 minutes.

[0068] 5) The white material obtained in 4) is first washed alternately with absolute ethanol and water.

[0069] 6) The final material obtained in 5) is dried in a vacuum drying oven at 60 °C for 32 hours to obtain bioactive particles.

[0070] 7) The bioactive particles are added to a n - hexane solution containing 15 wt% 3 - aminopropyltriethoxysilane at a mass - volume ratio of 3:100 g / mL, and stirred at 60 °C for 5 hours.

[0071] 8) The modified bioactive particles obtained in 7) are added to a pure n - hexane solution, washed twice repeatedly, and then dried at 60 °C for 5 hours for later use.

[0072] Part Two: Preparation of Bioactive Material with Both Anti - inflammatory and Repair Functions

[0073] 1) 0.8 g of sodium hyaluronate with a molecular weight of 120 kDa is added to 100 mL of aqueous solution, and stirred at 90 °C for 30 minutes to obtain Solution A1.

[0074] 2) 1 g of polyethylene glycol with a molecular weight of 6000 Da is added to 100 mL of aqueous solution, and stirred at 30 °C for 60 minutes to obtain Solution B1.

[0075] 3) Solution A1 and Solution B1 are mixed in a ratio of 1:1 to obtain Solution C, and stirred at room temperature for 60 minutes.

[0076] 4) 6 g of the modified bioactive particles and 0.06 g of cerium oxide particles are respectively weighed and added to Solution C, and stirred at room temperature for 6 hours for uniform reaction to obtain a bioactive material with both anti - inflammatory and repair functions.

[0077] Comparative Example 1

[0078] This comparative example provides a preparation method of a bioactive material, including the following steps:

[0079] Part One: Preparation of Bioactive Particles

[0080] 1) Absolute ethanol, water and ammonia water are mixed in a volume ratio of 45:53.2:1.8, stirred at 40 °C to obtain 500 mL of Solution A, the stirring time is 20 minutes, and the concentration of ammonia water is 25 - 28 wt%.

[0081] 2) 0.5 g of cetyltrimethylammonium bromide is weighed and added to Solution A in 1) to obtain Solution B, and stirred for 20 minutes.

[0082] 3) Slowly add 2 mL of tetraethyl orthosilicate to solution B, and continuously stir at 40 °C for 4 hours during the addition process to obtain a milky white solution.

[0083] 4) Centrifuge the milky white solution in step 3) to obtain a white material body, with a centrifugation rate of 4500 revolutions per minute and a centrifugation time of 30 minutes.

[0084] 5) First, alternately wash the white material body obtained in step 4) with absolute ethanol and water.

[0085] 6) Dry the final material body obtained in step 5) in a vacuum drying oven at 60 °C for 32 hours to obtain bioactive particles.

[0086] 7) Add the bioactive particles to a n - hexane solution containing 15 wt% 3 - aminopropyltriethoxysilane at a mass - volume ratio of 3:100 g / mL, and stir and react at 60 °C for 5 hours.

[0087] 8) Add the modified bioactive particles obtained in step 7) to a pure n - hexane solution, wash them repeatedly twice, and then dry them at 60 °C for 5 hours for later use.

[0088] Part Two: Preparation of Bioactive Materials with Both Anti - inflammatory and Repair Functions

[0089] 1) Add 0.8 g of sodium hyaluronate with a molecular weight of 120 kDa to 100 mL of aqueous solution, and stir at 90 °C for 30 minutes to obtain solution A1;

[0090] 2) Add 1 g of polyethylene glycol with a molecular weight of 6000 Da to 100 mL of aqueous solution, and stir at 30 °C for 60 minutes to obtain solution B1;

[0091] 3) Mix solution A1 and solution B1 in a 1:1 ratio to obtain solution C, and stir at room temperature for 60 minutes;

[0092] 4) Weigh 2 g of the modified bioactive particles and 0.3 g of cerium oxide particles respectively and add them to solution C, stir and react evenly at room temperature for 6 hours, and fully react to obtain bioactive materials with both anti - inflammatory and repair functions.

[0093] Comparative Example 2

[0094] This comparative example provides a preparation method of bioactive materials, including the following steps:

[0095] Part One: Preparation of Bioactive Particles

[0096] 1) Mix absolute ethanol, water, and ammonia water in a volume ratio of 45:53.2:1.8, stir at 40 °C to obtain 500 mL of solution A, with a stirring time of 20 minutes and the concentration of ammonia water being 25 - 28 wt%.

[0097] 2) Weigh 0.5 g of cetyltrimethylammonium bromide and add it to solution A in 1) to obtain solution B, and stir for 20 minutes.

[0098] 3) Slowly add 2 mL of tetraethyl orthosilicate to solution B, and continuously stir at 40 °C for 4 hours during the addition process to obtain a milky white solution.

[0099] 4) Centrifuge the milky white solution in 3) to obtain a white material, with a centrifuge speed of 4500 revolutions per minute and a centrifuge time of 30 minutes.

[0100] 5) First, alternately wash the white material obtained in 4) with absolute ethanol and water.

[0101] 6) Dry the final material obtained in 5) in a vacuum drying oven at 60 °C for 32 hours to obtain bioactive particles.

[0102] 7) Add the bioactive particles to a n - hexane solution containing 15 wt% 3 - aminopropyltriethoxysilane according to a mass - volume ratio of 3:100 g / mL, and stir and react at 60 °C for 5 hours.

[0103] 8) Add the modified bioactive particles obtained in 7) to a pure n - hexane solution, wash repeatedly twice, and then dry at 60 °C for 5 hours for use.

[0104] Part Two: Preparation of Bioactive Material with Both Anti - inflammatory and Repair Functions

[0105] 1) Add 0.8 g of sodium hyaluronate with a molecular weight of 120 kDa to 100 mL of aqueous solution, stir at 90 °C for 30 minutes to obtain solution A1;

[0106] 2) Add 1 g of polyethylene glycol with a molecular weight of 6000 Da to 100 mL of aqueous solution, stir at 30 °C for 60 minutes to obtain solution B1;

[0107] 3) Mix solution A1 and solution B1 in a ratio of 1:1 to obtain solution C, and stir at room temperature for 60 minutes;

[0108] 4) Weigh 25 g of the modified bioactive particles and 0.01 g of cerium oxide particles respectively, add them to solution C, stir and react evenly at room temperature for 6 hours, and fully react to obtain a bioactive material with both anti - inflammatory and repair functions.

[0109] Test experiment process for promoting endothelial cell proliferation: The bioactive material was extracted with serum-free medium at a concentration of 0.1 g / mL, and placed in a constant temperature shaker at 37 °C for shaking extraction to obtain the extract. Then, the CCK-8 kit was used to detect the viability of the cells. The seeding cell density was 10 4 cells / mL (500 μL / well). The extract and the cells were co-cultured for 1, 3, and 5 days. After the culture time ended, the old medium was aspirated, CCK-8 working solution was added, and incubated at 37 °C for 2 hours. After the reaction, it was transferred to a new 96-well plate. A multifunctional microplate reader was used to detect the absorbance value of the reaction solution at a wavelength of 450 nm.

[0110] Measurement of the absorbance value of the bioactive material for scavenging reactive oxygen species: An appropriate amount of DPPH powder was accurately weighed, dissolved in absolute ethanol and diluted to the required volume to prepare a 0.1 mmol / L DPPH ethanol solution. The prepared DPPH solution was transferred to a brown reagent bottle and stored in the dark for later use. Take several clean test tubes, and label them as the model group and the experimental group respectively. In the model group, absolute ethanol was added to the DPPH solution, and in the experimental group, a 1 wt% bioactive material solution was added to the DPPH solution. After vortex mixing evenly and reacting in the dark at room temperature, the absorbance value was measured at a wavelength of 517 nm using a UV-visible spectrophotometer.

[0111] Test of gene expression: The experiment was divided into three groups: the blank group, the model group, and the experimental group. RAW264.7 macrophages in a good adherent state were selected as the cells. The blank group was added with normal medium; the inflammatory model group was added with medium containing LPS to induce an inflammatory response; the experimental group was added with a 1 wt% concentration of the bioactive material. After the material and the cells were incubated for 1-2 hours, LPS was added and the culture was continued for 6-8 hours. After the culture ended, TRIzon reagent was used to extract the total cellular RNA of the cells, and NanoDrop was used to detect the purity and concentration of the RNA. Take an appropriate amount of RNA and operate according to the reverse transcription kit instructions to reverse transcribe the RNA into cDNA. Using the cDNA as a template, qRT-PCR reaction was carried out using SYBR Green PCR Master Mix for PCR amplification. After agarose gel electrophoresis analysis, it was confirmed that there was a target gene band. Finally, the cDNA was used for Real-Time PCR quantitative detection to obtain the influence of the test sample on the expression of inflammation-related genes.

[0112] Cytotoxicity experiment: L929 mouse fibroblasts were selected as the cells. After the material and the cells were co-cultured for 1 day, the old medium was discarded, CCK8 detection solution was added and incubated at 37 °C for 2 hours. After incubation, a microplate reader was used to measure the absorbance value of the reaction solution at 450 nm.

[0113] The above embodiments are preferred embodiments of the present invention, but the embodiments of the present invention are not limited to the above embodiments. Any other changes, modifications, substitutions, combinations, and simplifications made without departing from the spirit and principle of the present invention shall be equivalent replacement methods and are all included in the protection scope of the present invention.

Claims

1. A method for preparing a bioactive material having both anti-inflammatory and repairing properties, characterized in that: The following steps are involved: (1) adding hexadecyltrimethylammonium bromide to an ethanol / ammonia solution, mixing well, adding tetraethyl orthosilicate dropwise, stirring for reaction, centrifuging, washing, and drying to obtain bioactive particles; (2) adding the bioactive particles to an aminosilicone solution, reacting, washing, and drying to obtain amino-modified bioactive particles; (3) preparing a sodium hyaluronate solution and a polyethylene glycol solution respectively, then mixing the sodium hyaluronate solution and the polyethylene glycol solution evenly, adding the amino-modified bioactive particles and the cerium oxide particles, stirring and reacting, and obtaining a bioactive material.

2. The method for preparing a bioactive material having both anti-inflammatory and repairing properties according to claim 1, characterized in that: In the bioactive material of step (3), the concentrations of the aminated bioactive particles and the cerium oxide particles are 20-100 g / L and 0.1-1 g / L, respectively; And / or, the concentration of the sodium hyaluronate solution in step (3) is 0.1 to 10 g / L; And / or, the concentration of the polyethylene glycol solution in step (3) is 10 to 80 g / L; And / or, the volume ratio of the sodium hyaluronate solution to the polyethylene glycol solution in step (3) is 1:

1.

3. The method for preparing a bioactive material having both anti-inflammatory and repairing properties according to claim 1, characterized in that: In the bioactive material of step (3), the concentrations of the aminated bioactive particles and the cerium oxide particles are 25-30 g / L and 0.25-0.3 g / L, respectively; And / or, the concentration of the sodium hyaluronate solution in step (3) is 5 to 8 g / L; And / or, the concentration of the polyethylene glycol solution in step (3) is 10 to 30 g / L.

4. The method for preparing a bioactive material having both anti-inflammatory and repairing properties according to claim 1, characterized in that: The mass ratio of the bioactive particles to the aminosilicone in step (2) is 0.5-2:3-8; and / or, the aminosiloxane in step (2) is at least one of 3-aminopropyltriethoxysilane, γ-aminopropyltriethoxysilane, γ-aminopropyltrimethoxysilane, N-β-aminoethyl-γ-aminopropyltrimethoxysilane, N-β-aminoethyl-γ-aminopropyltriethoxysilane and N,N-dimethylaminopropylaminopropylmethyldimethoxysilane; And / or, the ratio of hexadecyltrimethylammonium bromide to ethyl orthosilicate in step (1) is 0.2-0.6 g:1-2.5 mL.

5. The method for preparing a bioactive material having both anti-inflammatory and repairing properties according to claim 1, characterized in that: The stirring reaction in step (1) is carried out at a temperature of 35 to 45° C. for 2 to 6 hours; And / or, the reaction temperature in step (2) is 60-65° C. and the reaction time is 4-8 hours; And / or, the stirring reaction in step (3) is carried out at room temperature for 2 to 6 hours.

6. The method for preparing a bioactive material having both anti-inflammatory and repairing properties according to claim 1, characterized in that: The ethanol / ammonia aqueous solution in step (1) is prepared by stirring anhydrous ethanol, water and ammonia aqueous solution at a volume ratio of (40-60): (55-59.2): (0.8-5) at 35-45° C. for 10-30 minutes; And / or, the ratio of the hexadecyltrimethylammonium bromide and the ethanol / ammonia mixed solution in step (1) is 0.2-0.6 g:500 mL; And / or, the concentration of the aminosiloxane solution in step (2) is 12-18 wt %, and the solvent is n-hexane.

7. The method for preparing a bioactive material having both anti-inflammatory and repairing properties according to claim 1, characterized in that: In step (3), the molecular weight of sodium hyaluronate is in the range of 100 to 1000 kDa; And / or, in step (3), the molecular weight of the polyethylene glycol is in the range of 200 to 6000 Da.

8. The method for preparing a bioactive material having both anti-inflammatory and repairing properties according to claim 1, characterized in that: The ratio of hexadecyltrimethylammonium bromide to ethyl orthosilicate in step (1) is 0.4-0.5 g:1.5-2 mL.

9. A bioactive material with both anti-inflammatory and repair properties obtained by the preparation method according to any one of claims 1 to 8.

10. Use of the bioactive material having both anti-inflammatory and repairing properties as claimed in claim 9 in the preparation of a product for treating damaged tissue repair.

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