Blood component quantitative preservation method and blood separator

Abstract

The invention relates to the technical field of blood separation, in particular to a quantitative preservation method for blood components and a blood segregage.The quantitative preservation method for the blood components comprises the steps that S1, a mother bag for containing blood is provided; s2, the mother bag is flatly extruded, the thickness of the mother bag is guaranteed to be consistent, and the mother bag is filled with blood; s3, the height of the mother bag is H, the height h1 of the plasma in the blood bag is detected, and the height h2 = H-h1 of red blood cells is calculated; s4, the mother bag is weighed again, and the weight m of the mother bag at the moment is recorded; s5, calculating the proportion of the red blood cells and the plasma in the mother bag according to the height ratio of the h1 to the h2, so as to obtain the weight of the red blood cells and the plasma; and S6, respectively extruding red blood cells and blood plasma with specified weights so as to ensure that the red blood cells and the blood plasma with the specified weights are reserved in the mother bag. Quantitative plasma and red blood cells can be accurately left in the mother bag, it is guaranteed that the albuginea cannot be extruded out along with the plasma, and the quality of a finished product produced in the albuginea separation procedure is guaranteed.

Description

Technical Field

[0001] This application relates to the technical field of blood separation, and particularly to a method for quantitatively retaining blood components and a blood separator. Background Art

[0002] With the development of technology, blood component separators have become key equipment for blood component separation. When blood needs to be separated, the blood is injected into a mother bag, and the blood is stratified in the mother bag. The upper layer is the plasma layer, the middle layer is the buffy coat (white blood cells and platelets), and the lower layer is the red blood cells. When separating the blood components in the mother bag, the method of squeezing the mother bag with a pressing plate is used for component separation. The plasma in the upper layer of the mother bag is squeezed out of the mother bag for collection, and the red blood cells in the lower layer of the mother bag are squeezed out of the mother bag for collection.

[0003] In the buffy coat separation procedure, a certain amount of plasma and a certain amount of red blood cells need to be retained in the mother bag. At this time, usually, all the plasma in the mother bag is squeezed out, then the red blood cells in the mother bag are weighed, and the excess red blood cells are squeezed out, leaving a certain amount of red blood cells. Then, a certain amount of plasma is squeezed into the mother bag, and the buffy coat separation procedure can be carried out.

[0004] However, during the process of squeezing out the plasma, the buffy coat will be squeezed out together with the plasma. After squeezing the plasma back in, the amount of buffy coat in the mother bag decreases, which will result in poor quality of the finished product produced by the buffy coat separation procedure. Summary of the Invention

[0005] The purpose of this application is to provide a method for quantitatively retaining blood components and a blood separator, which can accurately retain a certain amount of plasma and red blood cells in the mother bag and ensure that the buffy coat will not be squeezed out together with the plasma, thus ensuring the quality of the finished product produced by the buffy coat separation procedure.

[0006] In the first aspect, a method for quantitatively retaining blood components provided by this application adopts the following technical solution:

[0007] A method for quantitatively retaining blood components, comprising:

[0008] S1: Provide a mother bag for containing blood;

[0009] S2: Perform smooth extrusion on the mother bag to ensure that the thickness of the mother bag is consistent and the blood fills the mother bag;

[0010] S3: The height of the mother bag is H, and the height h of the plasma in the blood bag is detected 1 , and the height h of the red blood cells is calculated 2 = H - h 1 ;

[0011] S4: Weigh the mother bag again and record the weight m of the mother bag at this time;

[0012] S5: Calculate the proportion of red blood cells and plasma in the mother bag by the height ratio of h 1 and h 2 to obtain the weights of red blood cells and plasma;

[0013] S6: Squeeze out specified weights of red blood cells and plasma respectively to ensure that the specified weights of red blood cells and plasma are retained in the mother bag.

[0014] Optionally, in S2, hang the mother bag on the hanging rod of the blood separator, and squeeze the mother bag through the second squeezing plate and the first squeezing plate of the blood separator to make the thickness of the mother bag uniform.

[0015] Optionally, in S3, continuously squeeze the mother bag to squeeze out the plasma from the mother bag, causing the red blood cell liquid level to continuously rise. When the red blood cells cover the detection part on the blood separator, stop squeezing the mother bag, and obtain the height h of the plasma in the blood bag at this time through the distance between the hanging rod and the detection part. 1 .

[0016] Optionally, in S1, provide the mother bag and fix it.

[0017] Optionally, in S6, when squeezing out the specified weights of red blood cells and plasma, continuously weigh the mother bag to monitor the change in the weight of the mother bag.

[0018] In this application, by squeezing the mother bag flatly, the upper and lower thicknesses of the mother bag are made uniform, and the blood fills the mother bag. At this time, by calculating the height ratio of the plasma and red blood cells, the weight ratio of the plasma and red blood cells in the mother bag can be obtained. Since the weight of the mother bag can be measured at this time, the weights of the plasma and red blood cells can be obtained respectively, and then the excess red blood cells and plasma are squeezed out, leaving a fixed amount of red blood cells and plasma for the buffy coat separation procedure. Since it is not necessary to completely squeeze out the plasma separately during the process of squeezing out red blood cells and plasma, the buffy coat between the red blood cells and plasma will not be squeezed out together with the plasma, avoiding the loss of the buffy coat, thus ensuring the quality of the products produced by the buffy coat separation procedure.

[0019] And in this embodiment, a detection part is provided. When the red blood cells touch the detection part, stop squeezing the mother bag, and fix the distance between the detection part and the fixing place of the mother bag, thereby obtaining the height of the plasma. Since the height of the mother bag is fixed, the height of the red blood cells can be calculated. This measurement process can be automated without manual measurement, which is convenient and fast to use.

[0020] In a second aspect, a blood separator provided by this application adopts the following technical solution:

[0021] A blood separator, comprising

[0022] a separator body;

[0023] The extrusion mechanism is arranged on the separator body. The extrusion mechanism includes a driving member, a frame, a first extrusion plate, and a second extrusion plate. The first extrusion plate is fixed on the frame, and the output end of the driving member is fixed to the second extrusion plate and can drive the second extrusion plate to move in a direction close to or away from the first extrusion plate;

[0024] The weighing member, on which the frame is fixed;

[0025] The hanging rod is installed on the first extrusion plate for hanging the mother bag;

[0026] The detection member is installed on the first extrusion plate and is used to detect the liquid level height of red blood cells in the mother bag.

[0027] Optionally, a plurality of the detection members are provided, and the plurality of detection members are arranged in the vertical direction.

[0028] In this application, the extrusion mechanism is weighed by the weighing member, so as to continuously weigh the mother bag hung on the extrusion mechanism, continuously monitor the weight change of the mother bag, and the mother bag is extruded by the first extrusion plate and the second extrusion plate, so that the thickness of the mother bag is consistent up and down and the blood fills the mother bag, so as to facilitate calculating the proportion of red blood cells and plasma in the mother bag. The liquid level of red blood cells is detected by the detection member, so as to obtain the height of red blood cells and plasma, so as to convert the weight of plasma and red blood cells. Then, according to the cooperation of the second extrusion plate and the first extrusion plate, the excess plasma and red blood cells in the mother bag can be extruded to leave a fixed amount of plasma and red blood cells. And because the weighing member continuously monitors the weight of the mother bag, when squeezing out plasma or red blood cells, it is convenient to control the extrusion amount of plasma and red blood cells, and avoid the situation of excessive loss of plasma or red blood cells, thus ensuring the amount of plasma and red blood cells in the mother bag. Description of the Drawings

[0029] Figure 1 It is a schematic diagram of the overall structure of a blood separator according to an embodiment of the present application.

[0030] Figure 2 It is a schematic diagram of the structure of the extrusion mechanism according to an embodiment of the present application.

[0031] In the figure, 1, separator body; 2, extrusion mechanism; 21, first extrusion plate; 22, second extrusion plate; 23, driving member; 24, frame; 3, weighing member; 4, hanging rod; 5, detection member. Detailed Embodiments

[0032] The following will be described in further detail with reference to the attached Figure 1 - attached Figure 2 , to further illustrate the present application.

[0033] Embodiment 1:

[0034] A blood separator, referring to Figure 1 and Figure 2 , includes a separator body 1, an extrusion mechanism 2, a weighing member 3, a hanging rod 4, and a detection member 5. The extrusion mechanism 2 and the weighing member 3 are both installed on the separator body 1, and the extrusion mechanism 2 is fixed on the weighing member 3. The weighing member 3 is used to weigh the weight of the extrusion mechanism 2. The hanging rod 4 is installed on the extrusion mechanism 2, and the detection member 5 is also installed on the extrusion mechanism 2. In this embodiment, the weighing member 3 can be a weighing scale or other devices that can weigh in real time.

[0035] During use, hang the mother bag containing blood on the weighing member 3. At this time, the weighing member 3 weighs the weight of the blood in the mother bag, that is, the combined weight of plasma, red blood cells, and buffy coat. Since the weight of the buffy coat is very light and can be ignored, the weighed weight is the weight of plasma and red blood cells.

[0036] The extrusion mechanism 2 extrudes the mother bag. The upper end of the mother bag is connected to the plasma storage space, and the lower end of the mother bag is connected to the red blood cell storage space. In this embodiment, the plasma storage space can be a plasma bag or a plasma tank, and the red blood cell storage space can be a red blood cell bag or a red blood cell tank. Electric chucks for controlling on-off are installed between the mother bag and the plasma storage space and between the mother bag and the red blood cell storage space, so that the mother bag can separately extrude plasma or red blood cells during extrusion, facilitating the control of variables.

[0037] During extrusion, first disconnect the connection between the mother bag and the red blood cell storage space, so that plasma is extruded from the mother bag. During the extrusion of plasma, since the mother bag is continuously thinning, the liquid level of red blood cells is continuously rising. When the liquid level of red blood cells rises to cover the detection member 5, stop extruding the mother bag at this time.

[0038] The extrusion mechanism 2 extrudes the mother bag evenly, making the upper and lower thicknesses of the mother bag uniform. And because the extrusion mechanism 2 stops extruding the mother bag as soon as the red blood cells just cover the detection member 5, the distance between the detection member 5 and the hanging rod 4 at this time is the height of the plasma. Since both the detection member 5 and the hanging rod 4 are fixedly installed on the extrusion mechanism 2, the distance between the detection member 5 and the hanging rod 4 is a fixed value. Therefore, the height of the plasma h1 can be directly obtained at this time. Since the height of the mother bag is a fixed value H, the height of the red blood cells h2 = H - h1 can be directly calculated; the density of red blood cells and plasma is almost the same. At this time, since the thickness of the blood bag is the same up and down, the weight ratio of red blood cells and plasma in the mother bag can be calculated through the height ratio of red blood cells to plasma. And since the weight of the mother bag is directly weighed by the weighing member 3 at this time, the plasma weight and red blood cell weight can be directly calculated.

[0039] During the buffy coat separation process, a specific weight of plasma and red blood cells is required. At this time, the excess red blood cells or plasma are extruded separately, and a quantitative amount of plasma and red blood cells is retained to perform the buffy coat separation process. Since neither the plasma nor the red blood cells have been completely extruded during the extrusion process of the plasma and red blood cells, the buffy coat between the plasma and the red blood cells is retained to the greatest extent possible with basically no consumption, thereby increasing the quality of the finished product produced by the buffy coat separation process.

[0040] In this embodiment, the detection member 5 is a liquid level sensor. When the red blood cells just cover the liquid level sensor, the extrusion mechanism 2 stops extruding the mother bag. In other embodiments, the detection member 5 can also be an infrared sensor or other devices capable of detecting the liquid level of red blood cells.

[0041] Furthermore, a plurality of detection members 5 are provided, and the plurality of detection members 5 are evenly spaced in the vertical direction. When hanging mother bags of different specifications, the liquid level height of the red blood cells in the mother bag is slightly different. There may be a situation where after hanging the mother bag, the red blood cells have already covered the detection member 5. By providing a plurality of detection members 5, after hanging the mother bag, first determine how many detection members 5 are covered by the red blood cells, and then control the extrusion mechanism 2 through the uncovered detection members 5 to perform the above extrusion process.

[0042] Specifically, taking five detection members 5 as an example, the five detection members 5 are respectively named the first detection member, the second detection member, the third detection member, the fourth detection member, and the fifth detection member. The first detection member, the second detection member, the third detection member, the fourth detection member, and the fifth detection member are arranged in sequence in the vertical direction. After the mother bag is hung on the hanging rod 4, the red blood cells in the mother bag cover the fourth detection member and the fifth detection member. At this time, the signals of the covered fourth detection member and the fifth detection member are collected, and the signals of the uncovered first detection member, the second detection member, and the third detection member are also collected. Thus, it is determined that the detection member 5 closest to the red blood cell liquid level and not covered is the third detection member. Therefore, the third detection member 5 is used to detect the liquid level of the red blood cells. When the red blood cell liquid level covers the third detection member, the extrusion mechanism 2 stops extruding the mother bag.

[0043] Specifically, the blood separator further includes a controller. A plurality of detection members 5 are all electrically connected to the controller, and the controller is electrically connected to the extrusion mechanism 2. After the mother bag is hung on the hanging rod 4, at this time, the mother bag covers some of the detection members 5. The controller receives the signals transmitted by the plurality of detection members 5, and by analyzing the signals, determines which uncovered detection member 5 is closest to the red blood cell liquid level. When during the extrusion process, the red blood cells cover this detection member 5, the controller controls the extrusion mechanism 2 to stop operating. In other embodiments, a gravity sensor can be provided on the hanging rod 4, and the gravity sensor is electrically connected to the controller. When the mother bag is hung on the hanging rod 4, the gravity sensor transmits a signal to the controller, and the controller starts to analyze the detection members 5 to determine which detection member 5 not covered by the red blood cells is closest to the red blood cell liquid level.

[0044] The extrusion mechanism 2 includes a driving member 23, a frame 24, a first extrusion plate 21 and a second extrusion plate 22. The driving member 23 is electrically connected to the controller, and the operation of the driving member 23 is controlled by the controller. The frame 24 is fixedly installed on the separator body 1. The first extrusion plate 21 is fixed on the frame 24, the hanging rod 4 is fixed on the first extrusion plate 21, the driving member 23 is fixed on the frame 24 and the output end is fixedly connected to the second extrusion plate 22. The driving member 23 is used to drive the second extrusion plate 22 to move in a direction close to or away from the first extrusion plate 21.

[0045] In this embodiment, the surfaces of the first extrusion plate 21 and the second extrusion plate 22 that face each other are both set as flat planes. When the second extrusion plate 22 moves closer to the first extrusion plate 21, the mother bag is extruded, the air in the mother bag is squeezed out of the mother bag and the plasma or red blood cells are squeezed out of the mother bag, so that a certain amount of plasma and red blood cells are retained in the mother bag, facilitating the buffy coat separation procedure.

[0046] In this embodiment, the driving member 23 adopts a linear guide rail to drive the second extrusion plate 22 to move in a direction close to or away from the first extrusion plate 21. In other embodiments, the driving member 23 can also adopt a hydraulic cylinder or other devices that can drive the second extrusion plate 22 to move.

[0047] Embodiment 2:

[0048] A method for quantitatively retaining blood components:

[0049] S1: Provide a mother bag for containing blood and fix the mother bag. In this embodiment, the blood is already stratified blood, with plasma on the upper layer, buffy coat (platelets and white blood cells) in the middle, and red blood cells on the lower layer. The mother bag can be fixed by hanging the mother bag on the hanging rod 4. In other embodiments, the mother bag can also be fixed by a fixture or other means.

[0050] S2: Extrude the mother bag smoothly to ensure that the thickness of the mother bag is uniform and the blood fills the mother bag.

[0051] When the mother bag is extruded smoothly, at this time, the red blood cells are disconnected from the red blood cell storage space, and the plasma is connected to the plasma storage space, thereby squeezing the air and part of the plasma in the mother bag out of the mother bag, making the blood fill the entire mother bag and making the upper and lower thicknesses of the mother bag uniform, so as to facilitate the calculation of the weights of red blood cells and plasma. In this embodiment, the second extrusion plate 22 is moved closer to the first extrusion plate 21 to extrude the mother bag by the second extrusion plate 22 squeezing the first extrusion plate 21. In other embodiments, the mother bag can be extruded by the first extrusion plate 21 and the second extrusion plate 22 moving closer at the same time, or other extrusion methods can be used, as long as all the air in the mother bag is squeezed out and the thickness of the mother bag is uniform.

[0052] S3: The height of the mother bag is H, and the height h of the plasma in the blood bag is detected. 1 , and the height h of the red blood cells is calculated 2 =H - h 1 ;

[0053] In S3, continuously squeeze the mother bag to squeeze the plasma out of the mother bag, causing the liquid level of the red blood cells to continuously rise. When the red blood cells cover the detection part 5 on the blood separator, stop squeezing the mother bag. Through the distance between the hanging rod 4 and the detection part 5, the height h of the plasma in the blood bag at this time can be obtained. 1 , and the liquid level height of the red blood cells can be automatically calculated by the controller without manual measurement, reducing errors and facilitating use. It only needs to be hung on the hanging rod 4, and the rest of the steps can be automatically carried out by the equipment.

[0054] By setting the detection part 5, when the liquid level of the red blood cells covers the detection part 5, the squeezing of the mother bag can be automatically stopped. And because the distance between the detection part 5 and the hanging rod 4 is fixed, the height of the plasma can be automatically obtained and calculated.

[0055] S4: Weigh the mother bag again and record the weight m of the mother bag at this time;

[0056] Since the squeezing mechanism 2 is arranged on the weighing part 3, the weighing part 3 continuously detects the weight of the mother bag and records the weight of the mother bag at this time, which is convenient for calculating the weights of the red blood cells and plasma in S5.

[0057] S5: Calculate the proportions of the red blood cells and plasma in the mother bag through the height ratio of h 1 and h 2 , so as to obtain the weights of the red blood cells and plasma;

[0058] S6: Squeeze out the specified weights of the red blood cells and plasma respectively to ensure that the specified weights of the red blood cells and plasma are retained in the mother bag.

[0059] When performing the buffy coat separation procedure, a certain amount of red blood cells and plasma are required. At this time, after calculating the weights of the red blood cells and plasma in the mother bag, according to the actual needs, the excess red blood cells and plasma are squeezed out. Since the weighing part 3 continuously weighs the mother bag, the extrusion amounts of the plasma and red blood cells are continuously monitored to avoid over-extrusion, ensuring that the amounts of the remaining red blood cells and plasma in the mother bag are the same as expected, thus ensuring the product quality of the buffy coat separation procedure.

[0060] The embodiments of this specific implementation manner are all preferred embodiments of this application, and do not limit the protection scope of this application accordingly. The same components are denoted by the same reference numerals. Therefore, all equivalent changes made according to the structure, shape, and principle of this application should be covered within the protection scope of this application.

Claims

1. A method for quantitative retention of blood components, characterized in that: include: S1: Provide mother bag for holding blood; S2: Squeeze the mother bag evenly to ensure that the thickness of the mother bag is uniform and the blood fills the mother bag; S3: The height of the mother bag is H, the height h1 of the plasma in the blood bag is detected, and the height of the red blood cells is calculated as h2 = H-h1; S4: Weigh the mother bag again and record the weight m of the mother bag at this time; S5: Calculate the proportion of red blood cells and plasma in the mother bag by the height ratio of h1 and h2, and thus obtain the weight of red blood cells and plasma; S6: The designated weights of red blood cells and plasma are squeezed out respectively to ensure that the designated weights of red blood cells and plasma are retained in the mother bag.

2. A method for quantitative storage of blood components according to claim 1, characterized in that: In S2, the mother bag is hung on the hanging rod (4) of the blood separator, and the mother bag is squeezed by the second squeezing plate (22) and the first squeezing plate (21) of the blood separator to achieve a uniform thickness of the mother bag.

3. A method for quantitative storage of blood components according to claim 2, characterized in that: In S3, the mother bag is continuously squeezed to squeeze the plasma out of the mother bag, so that the red blood cell level continues to rise. When the red blood cells cover the detection piece (5) on the blood separator, the squeezing of the mother bag is stopped, and the height h1 of the plasma in the blood bag at this time is obtained through the distance between the hanging rod (4) and the detection piece (5).

4. A method for quantitative storage of blood components according to claim 1, characterized in that: In S6, when the specified weight of red blood cells and plasma are squeezed out, the mother bag is continuously weighed to monitor the weight change of the mother bag.

5. A blood separator, characterized in that: include Separator body (1); A squeezing mechanism (2) is arranged on the separator body (1), the squeezing mechanism (2) comprising a driving member (23), a frame (24), a first squeezing plate (21) and a second squeezing plate (22), the first squeezing plate (21) being fixed on the frame (24), an output end of the driving member (23) being fixed to the second squeezing plate (22) and being able to drive the second squeezing plate (22) to move in a direction close to or away from the first squeezing plate (21); A weighing piece (3), the frame (24) being fixed on the weighing piece (3); A hanging rod (4), mounted on the first extrusion plate (21) for hanging the mother bag; The detection member (5) is installed on the first squeezing plate (21) and is used to detect the liquid level of the red blood cells in the mother bag.

6. A blood separator according to claim 5, characterized in that: A plurality of the detection members (5) are provided, and the plurality of the detection members (5) are arranged in a vertical direction.

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