A cultivation method using morchella raw soil

Abstract

This invention discloses a cultivation method using undisturbed soil from wild morel mushroom cultivation sites, belonging to the field of morel mushroom cultivation technology. The method involves excavating undisturbed soil from wild morel mushroom growing areas, preparing mushroom beds in cultivation areas, transferring the undisturbed soil to the prepared mushroom beds, leveling it, spraying with a composite film-forming liquid, and compacting it. Nutrient bags containing morel mushroom substrate are placed on the prepared undisturbed soil. During cultivation, an arched shed is constructed, controlling the temperature at 16-18℃ and the humidity at 60-75%, and cultivating morels under conditions of 500-700 lumens of light flux. Ventilation is performed every two days. Morels can be harvested when the cap diameter reaches 5-7 cm. This method preserves the microbial community and nutrients in the undisturbed soil, making it suitable for the growth and reproduction of wild morels, thereby improving the cultivation efficiency of wild morels and protecting wild morel resources.

Description

Technical Field

[0001] This invention belongs to the field of morel cultivation technology, specifically relating to a cultivation method using undisturbed soil for morel cultivation. Background Technology

[0002] Morel mushrooms are a prized edible fungus. Their caps are nearly spherical, oval, or elliptical, with a blunt, rounded apex and pitted surfaces resembling a sheep's stomach. Morels primarily grow in the humus layer of broad-leaved forests or mixed coniferous and broad-leaved forests, mainly in humus-rich sandy loam or brown soils. They are particularly prone to occur on burned forest floors. Furthermore, morels prefer moist conditions, and their growth environment must be kept humid. Temperatures of 4-16°C within a few weeks in early spring can stimulate the formation of morel fruiting bodies.

[0003] Morel mushrooms are rich in nutrients, containing vitamins, minerals, amino acids, proteins, carbohydrates, and other nutrients. These nutrients help replenish the body's needs for nutrition and energy, improve appetite, enhance immunity, and promote metabolism. Modern research has also found that morel mushrooms contain polysaccharides, which have anti-cancer and anti-tumor effects.

[0004] Cultivating morel mushrooms requires certain techniques and conditions. During cultivation, attention must be paid to humidity, temperature, and pest and disease control. However, current cultivation methods generally require re-mixing the soil, adjusting soil pH, and manual inoculation, which is not conducive to improving the cultivation efficiency of morel mushrooms. Summary of the Invention

[0005] The purpose of this invention is to provide a cultivation method using undisturbed soil for morel mushrooms. This method makes full use of wild resources, preserves the microbial community and nutrients in the undisturbed soil, and can be used for the growth and reproduction of wild morel mushrooms, thereby improving the cultivation efficiency of wild morel mushrooms and protecting wild morel mushroom resources.

[0006] The objective of this invention can be achieved through the following technical solutions:

[0007] A cultivation method using undisturbed soil for morel mushrooms includes the following steps:

[0008] Step 1: At the wild morel mushroom growing site, dig up a 10-20cm layer of undisturbed soil from the top of the soil to use as cultivation soil; prepare the mushroom bed in the cultivation site, transfer the collected undisturbed soil from the wild morel growing site to the dug mushroom bed, spread it evenly, and apply 1-1.5kg / m² of soil. 2 The amount of composite film-forming liquid is sprayed and compacted.

[0009] Step 2: Place nutrient bags on the prepared soil for morel mushroom cultivation. The amount of nutrient bags used is 1500-2000 kg / mu. The nutrient bags are filled with morel mushroom feed, which is made by mixing rice husks and wheat in a 1:3 mass ratio and then sterilizing at high temperature. The moisture content is 45%.

[0010] Step 3: During cultivation, construct an arched shed, control the temperature at 16-18℃ and the humidity at 60-75%, and cultivate morel mushrooms under conditions of 500-700 lumens of light flux. Ventilate once every 2 days to prevent mold growth. Harvest when the diameter of the morel mushroom cap is 5-7cm, thus completing the cultivation method using the original soil of morel mushrooms.

[0011] Furthermore, the composite film-forming solution is prepared through the following steps:

[0012] Step 1: Dissolve polyethylene glycol in dichloromethane and transfer it to a reaction vessel. Then, add a 15% (w / w) solution of triethylamine in p-toluenesulfonyl chloride to the reaction vessel. Keep the reaction at 20-25℃ for 10-12 hours. Wash the reaction solution 2-3 times with a 3 mol / L hydrochloric acid solution. Combine the organic phases and adjust the pH to 7 with sodium bicarbonate. Filter the solution and concentrate the filtrate under reduced pressure to obtain esterified polyethylene glycol.

[0013] Step 2: Add esterified polyethylene glycol and 28% ammonia water to a reaction vessel and react at 135-140℃ for 5-6 hours. Extract the reaction product three times with DCM. Combine the organic phases and adjust the pH to 11.5-12 with 1 mol / L sodium hydroxide solution. Stir at 20-25℃ and 200-300 r / min for 4-5 hours. Let stand, separate the organic phase and wash with saturated sodium chloride solution until neutral. Filter, dry the filtrate with anhydrous sodium sulfate to remove water, let stand, filter, and concentrate under reduced pressure to obtain amino-terminated polyethylene glycol.

[0014] Step 3: Add amino-terminated polyethylene glycol, lignin nanoparticles, potassium humate and deionized water to the reaction vessel and stir for 30-40 min at 70-80 and 200-300 r / min. Then add dialdehyde starch, anhydrous methanol, boric acid, glutaraldehyde and acetic acid solution with a mass fraction of 10% to the reaction vessel and continue stirring for 20-30 min to obtain the composite film-forming solution.

[0015] Furthermore, in step 1, the mass ratio of polyethylene glycol, dichloromethane, and triethylamine solution of p-toluenesulfonyl chloride is 2:10:5-6.

[0016] Furthermore, in step 2, the mass ratio of esterified polyethylene glycol to ammonia is 1:4.

[0017] Furthermore, in step 3, the mass ratio of the terminal amino polyethylene glycol, lignin nanoparticles, potassium humate, deionized water, dialdehyde starch, anhydrous methanol, boric acid, glutaraldehyde, and acetic acid solution is 1:2:10:250:20:30:10:10:20.

[0018] Furthermore, lignin nanoparticles are prepared via the following steps:

[0019] Ammonium lignosulfonate and deionized water were added to a reaction vessel and stirred to dissolve. Anhydrous ethanol was then added dropwise to the reaction vessel under stirring conditions of 200-300 r / min, which initiated the assembly of ammonium lignosulfonate into lignin nanoparticles with uniform particle size. After the addition was completed, the anhydrous ethanol was removed by rotary evaporation and the nanoparticles were obtained by vacuum drying.

[0020] Furthermore, the ratio of ammonium lignosulfonate, deionized water, and anhydrous ethanol is 0.1g:15mL:25-30mL.

[0021] The beneficial effects of this invention are:

[0022] This invention utilizes a cultivation method based on the original soil of morel mushrooms, making full use of wild resources and preserving the microbial community and nutrients in the original soil. This method can be used for the growth and reproduction of wild morel mushrooms, thereby improving the cultivation efficiency of wild morel mushrooms and protecting wild morel mushroom resources.

[0023] In this invention, the aldehyde groups in the dialdehyde starch and glutaraldehyde in the composite film-forming solution can crosslink with the amino groups in the amino-terminated polyethylene glycol, while boric acid can crosslink with raw materials such as lignin nanoparticles, which helps in the formation of a three-dimensional network and improves the stability of the crosslinked gel film. Treatment of the original morel mushroom soil with the composite film-forming solution helps increase the organic matter and other essential substances in the cultivation soil, improves soil structure, enhances its water and fertilizer retention capacity, and promotes the growth and reproduction of wild morel mushrooms. Detailed Implementation

[0024] The technical solutions of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0025] Example 1: This example provides a cultivation method using undisturbed soil for morel mushrooms, including the following steps:

[0026] S1: Add 1 kg of ammonium lignosulfonate and 150 L of deionized water to a reaction vessel and stir to dissolve. Under stirring at 300 r / min, add 300 L of anhydrous ethanol dropwise to the reaction vessel to initiate the assembly of ammonium lignosulfonate into lignin nanoparticles with uniform particle size. After the addition is complete, remove the anhydrous ethanol by rotary evaporation and vacuum dry to obtain lignin nanoparticles.

[0027] S2: Dissolve 2 kg of polyethylene glycol in 10 kg of dichloromethane and transfer it to a reaction vessel. Then, add 6 kg of a 15% (w / w) solution of triethylamine in p-toluenesulfonyl chloride to the reaction vessel. Keep the reaction vessel at 25°C for 12 h. Wash the reaction solution 2-3 times with a 3 mol / L hydrochloric acid solution. Combine the organic phases and adjust the pH to 7 with sodium bicarbonate. Filter the solution and concentrate the filtrate under reduced pressure to obtain esterified polyethylene glycol.

[0028] S3: Add 1 kg of esterified polyethylene glycol and 4 kg of 28% ammonia water to a reactor and react at 140°C for 6 h. Extract the reaction product three times with DCM. Combine the organic phases and adjust the pH to 12 with 1 mol / L sodium hydroxide solution. Stir at 25°C and 300 r / min for 5 h. Let stand, separate the organic phase and wash with saturated sodium chloride solution until neutral. Filter, dry the filtrate with anhydrous sodium sulfate to remove water, let stand, filter, and concentrate under reduced pressure to obtain amino-terminated polyethylene glycol.

[0029] S4: Add 100g of amino-terminated polyethylene glycol, 200g of lignin nanoparticles, 1kg of potassium humate and 25kg of deionized water to a reaction vessel and stir for 40min at 80 and 300r / min. Then add 2kg of dialdehyde starch, 3kg of anhydrous methanol, 1kg of boric acid, 1kg of glutaraldehyde and 2kg of 10% acetic acid solution to the reaction vessel and continue stirring for 30min to obtain the composite film-forming solution.

[0030] S5: Due to the need for high-temperature summer treatment, the cultivation site should be selected under the forest or in a shady area. The requirements for the cultivation site are: First, convenient transportation, clean and sufficient water source, easy operation of rotary tiller and ventilation; Second, deep soil layer, rich in humus, preferably loose loam with convenient drainage and irrigation; Third, no edible fungi such as king oyster mushroom or bamboo fungus have been grown in the past 5 years.

[0031] S6: Uncirculated Soil Sampling: Upon discovery of wild morel mushrooms, immediately collect undisturbed soil samples from a 0-20cm radius around the mushrooms, taking only a semi-circular sample and preserving the undisturbed soil within the semi-circle. After sampling, fill the area with surrounding soil to minimize disruption to the wild morel mushrooms' habitat and protect the wild morel mushroom resource. The amount of undisturbed soil collected depends on the number and area of ​​wild morel mushrooms, typically 5-10kg.

[0032] S7: (1) Site preparation: Clean up stones, weeds, agricultural film and other debris in the cultivation site, and spread 60 kg of quicklime per mu for disinfection. Deep plow the soil to 25 cm-30 cm, and control the soil particle size to 1 cm-3 cm. According to the conditions of the cultivation site, dig drainage ditches around the perimeter, 40 cm wide and 60 cm deep.

[0033] (2) Digging the pit: Based on the weight of the collected undisturbed soil, dig a rectangular pit 5cm deep, 100cm long, and 50cm wide. Reserve 50m² for future expansion. 2 .

[0034] (3) Cultivation: ① Spring wild morel mushrooms: After collecting the original soil in the first year, spread it evenly in a rectangular pit to a height of 5-8cm, according to 1.5kg / m 2 Spray the compound film-forming liquid and compact it, then cover with a double layer of shade netting, maintaining soil moisture at 25% (water appropriately when the soil dries and turns white). After summer treatment (cover the shade netting with a layer of straw, then add an arched shed 30-50cm high, and then cover with another layer of shade netting); from late September to mid-October, when white or pale yellow bacterial frost is visible on the soil surface, remove the surface shade netting and straw, and add nutrient bags (4 bags / m²). 2 In early December, cover the soil with a black plastic film to retain heat and moisture. The film should have evenly spaced holes (2-3 cm in diameter) spaced 25-35 cm apart to increase air permeability. In mid-February of the following year, as temperatures begin to rise, remove the black film, water thoroughly to stimulate mushroom growth. Mushrooms will begin to appear from late February to late March, and harvesting will end in late April. After harvesting, remove all nutrient bags without disturbing the original soil. Then, cover with a double layer of shade netting, replace the arched shed and shade netting, and maintain soil moisture at 20-25% (water appropriately when the soil dries out and turns white). After summer treatment (cover the shade netting with a layer of straw, add an arched shed 30-50 cm high, and then cover with another layer of shade netting); in mid-September, till the soil to a depth of 25-30 cm, controlling the soil particle size to 1-3 cm. From late September to mid-October, when white or pale yellow mycelial bloom is visible on the soil surface, remove the top layer of shade netting and straw, and add nutrient bags (4 bags / m²). 2 In early December, cover the mushrooms with a black plastic film to retain heat and moisture. The film should have small holes (2-3 cm in diameter) evenly spaced 30 cm apart to increase air permeability. In mid-February of the third year, as temperatures begin to rise, remove the black film, water thoroughly to stimulate mushroom growth. Mushrooms will begin to appear from late February to late March, and harvesting will end in late April, yielding 2-3 flushes. Subsequent management is the same as the second year, and so on. The cultivation area gradually expands each year; for example, if the first year's area is 0.5 m²... 2 In the second year, it can reach 2-3m. 2 It can reach 8m in the third year. 2 In the fourth year, it can reach 10-15m. 2Following the above steps, maintain soil moisture to preserve mycelial vitality and quality, allowing for continuous expansion of the planting area. Avoid spraying pesticides and fertilizers. Remove weeds manually as needed. Yields will be lower in the second year, but will reach an average of 0.30 kg / m² in the third year and beyond. ② Autumn Wild Morel Mushrooms: After collecting the original soil from the first year, spread it evenly in rectangular pits to a height of 5-8 cm. Water thoroughly, construct a 50 cm high arched shed, and cover with a shade net. Maintain soil moisture at 20-25% (water appropriately when the soil dries out and turns white). Add nutrient bags (6 bags / m²) 20 days after transplanting. 2 In early December, cover the soil with a black film to retain heat and moisture. The black film should have evenly spaced holes (2-3 cm in diameter, 30 cm apart) to increase air permeability. In March of the following year, when temperatures rise, remove the black film. Maintain soil moisture at 20-25% and allow it to overwinter (cover the soil surface with a double layer of shade netting and a layer of straw, retaining the original arched shed and covering it with another layer of shade netting). Continue this treatment until mid-September, when temperatures drop to 25°C or below. Remove the shade netting and straw, and water thoroughly to stimulate mushroom growth. Mushrooms should appear in early to mid-October. Harvest from late November to early December. Till the soil to a depth of 30 cm, ensuring the soil particles are broken down to 2 cm. After 12 days, when a white or pale yellow mycelial bloom is visible on the soil surface, add nutrient bags (6 bags / m²). 2 Cover with black film (as above), then repeat as above, and so on. Management is the same as for wild morel mushrooms in spring. Yield is lower in the second year, but from the third year onwards, the average yield can reach 0.45 kg / m³. 2 .

[0035] Example 2: This example provides a cultivation method using undisturbed soil for morel mushrooms, including the following steps:

[0036] S1: Add 1 kg of ammonium lignosulfonate and 150 L of deionized water to a reaction vessel and stir to dissolve. Under stirring at 200 r / min, add 250 L of anhydrous ethanol dropwise to the reaction vessel to initiate the assembly of ammonium lignosulfonate into lignin nanoparticles with uniform particle size. After the addition is complete, remove the anhydrous ethanol by rotary evaporation and vacuum dry to obtain lignin nanoparticles.

[0037] S2: Dissolve 2 kg of polyethylene glycol in 10 kg of dichloromethane and transfer it to a reaction vessel. Then, add 5 kg of a 15% (w / w) solution of triethylamine in p-toluenesulfonyl chloride to the reaction vessel. Keep the reaction at 20 °C for 10 h. Wash the reaction solution twice with a 3 mol / L hydrochloric acid solution. Combine the organic phases and adjust the pH to 7 with sodium bicarbonate. Filter the solution and concentrate the filtrate under reduced pressure to obtain esterified polyethylene glycol.

[0038] S3: Add 1 kg of esterified polyethylene glycol and 4 kg of 28% ammonia water to a reactor and react at 135-140℃ for 5 h. Extract the reaction product three times with DCM. Combine the organic phases and adjust the pH to 11.5 with sodium hydroxide solution with a molar concentration of 1 mol / L. Stir at 20℃ and 200 r / min for 4-5 h. Let stand, separate the organic phase and wash with saturated sodium chloride solution until neutral. Filter, dry the filtrate with anhydrous sodium sulfate to remove water, let stand, filter, and concentrate under reduced pressure to obtain amino-terminated polyethylene glycol.

[0039] S4: Add 100g of amino-terminated polyethylene glycol, 200g of lignin nanoparticles, 1kg of potassium humate and 25kg of deionized water to a reaction vessel and stir for 30-40min at 70 and 200r / min. Then add 2kg of dialdehyde starch, 3kg of anhydrous methanol, 1kg of boric acid, 1kg of glutaraldehyde and 2kg of 10% acetic acid solution to the reaction vessel and continue stirring for 20-30min to obtain the composite film-forming solution.

[0040] S5: At the wild morel mushroom growing site, dig up the top 10cm layer of undisturbed morel soil to use as cultivation soil; dig mushroom beds at the cultivation site, transfer the collected undisturbed morel soil from the wild morel growing site to the dug mushroom beds, level it, and apply 1kg / m² of soil. 2 The amount of composite film-forming liquid is sprayed and compacted.

[0041] The original soil in the growing area of ​​wild morel mushrooms is rich in morel mycelium, as well as the necessary microbial community and nutrients, which is conducive to the growth of morel mushrooms.

[0042] S6: Place nutrient bags on the prepared undisturbed soil for morel mushroom cultivation. The amount of nutrient bags used is 1500 kg / mu. The nutrient bags are filled with morel mushroom feed, which is obtained by mixing rice husks and wheat in a mass ratio of 1:3 and then sterilizing at high temperature. The moisture content is 45%.

[0043] S7: During cultivation, build an arched shed, control the temperature at 16℃ and the humidity at 60%, and plant morel mushrooms under a light flux of 500 lumens. Ventilate once every 2 days to prevent mold growth. Harvest when the diameter of the morel cap is 5cm. This completes the cultivation method using the original soil of morel mushrooms.

[0044] Example 3: This example provides a cultivation method using undisturbed soil for morel mushrooms, including the following steps:

[0045] S1: Add 1 kg of ammonium lignin sulfonate and 150 L of deionized water to a reaction vessel and stir to dissolve. Under stirring at 250 r / min, add 280 L of anhydrous ethanol dropwise to the reaction vessel to initiate the assembly of ammonium lignin sulfonate into lignin nanoparticles with uniform particle size. After the addition is complete, remove the anhydrous ethanol by rotary evaporation and vacuum dry to obtain lignin nanoparticles.

[0046] S2: Dissolve 2 kg of polyethylene glycol in 10 kg of dichloromethane and transfer it to a reaction vessel. Then, add 5.5 kg of a 15% (w / w) solution of triethylamine in p-toluenesulfonyl chloride to the reaction vessel. Keep the reaction vessel at 22°C for 11 h. Wash the reaction solution twice with a 3 mol / L hydrochloric acid solution. Combine the organic phases and adjust the pH to 7 with sodium bicarbonate. Filter the solution and concentrate the filtrate under reduced pressure to obtain esterified polyethylene glycol.

[0047] S3: Add 1 kg of esterified polyethylene glycol and 4 kg of 28% ammonia water to a reactor and react at 138°C for 5.5 h. Extract the reaction product three times with DCM. Combine the organic phases and adjust the pH to 11.8 with 1 mol / L sodium hydroxide solution. Stir at 22°C and 250 r / min for 4.5 h. Let stand, separate the organic phase and wash with saturated sodium chloride solution until neutral. Filter, dry the filtrate with anhydrous sodium sulfate to remove water, let stand, filter, and concentrate under reduced pressure to obtain amino-terminated polyethylene glycol.

[0048] S4: Add 100g of amino-terminated polyethylene glycol, 200g of lignin nanoparticles, 1kg of potassium humate and 25kg of deionized water to the reactor and stir for 35min at 75 and 250r / min. Then add 2kg of dialdehyde starch, 3kg of anhydrous methanol, 1kg of boric acid, 1kg of glutaraldehyde and 2kg of 10% acetic acid solution to the reactor and continue stirring for 25min to obtain the composite film-forming solution.

[0049] S5: At the wild morel mushroom growing site, dig up the top 15cm layer of undisturbed soil to use as cultivation soil; dig mushroom beds at the cultivation site, transfer the collected undisturbed soil from the wild morel mushroom growing site to the dug mushroom beds, level it, and apply 1.2kg / m² of soil. 2 The amount of composite film-forming liquid is sprayed and compacted.

[0050] S6: Place nutrient bags on the prepared undisturbed soil for morel mushroom cultivation. The amount of nutrient bags used is 1800 kg / mu. The nutrient bags are filled with morel mushroom feed, which is obtained by mixing rice husks and wheat in a mass ratio of 1:3 and then sterilizing at high temperature. The moisture content is 45%.

[0051] S7: During cultivation, build an arched shed, control the temperature at 17℃ and the humidity at 70%, and plant morel mushrooms under a light flux of 650 lumens. Ventilate once every 2 days to prevent mold growth. When the diameter of the morel cap is 6cm, it can be harvested, thus completing the cultivation method using the original soil of morel mushrooms.

[0052] Example 4: This example provides a cultivation method using undisturbed soil for morel mushrooms, including the following steps:

[0053] S1: Add 1 kg of ammonium lignosulfonate and 150 L of deionized water to a reaction vessel and stir to dissolve. Under stirring at 300 r / min, add 300 L of anhydrous ethanol dropwise to the reaction vessel to initiate the assembly of ammonium lignosulfonate into lignin nanoparticles with uniform particle size. After the addition is complete, remove the anhydrous ethanol by rotary evaporation and vacuum dry to obtain lignin nanoparticles.

[0054] S2: Dissolve 2 kg of polyethylene glycol in 10 kg of dichloromethane and transfer it to a reaction vessel. Then, add 6 kg of a 15% (w / w) solution of triethylamine in p-toluenesulfonyl chloride to the reaction vessel. Keep the reaction vessel at 25°C for 12 h. Wash the reaction solution 2-3 times with a 3 mol / L hydrochloric acid solution. Combine the organic phases and adjust the pH to 7 with sodium bicarbonate. Filter the solution and concentrate the filtrate under reduced pressure to obtain esterified polyethylene glycol.

[0055] S3: Add 1 kg of esterified polyethylene glycol and 4 kg of 28% ammonia water to a reactor and react at 140°C for 6 h. Extract the reaction product three times with DCM. Combine the organic phases and adjust the pH to 12 with 1 mol / L sodium hydroxide solution. Stir at 25°C and 300 r / min for 5 h. Let stand, separate the organic phase and wash with saturated sodium chloride solution until neutral. Filter, dry the filtrate with anhydrous sodium sulfate to remove water, let stand, filter, and concentrate under reduced pressure to obtain amino-terminated polyethylene glycol.

[0056] S4: Add 100g of amino-terminated polyethylene glycol, 200g of lignin nanoparticles, 1kg of potassium humate and 25kg of deionized water to a reaction vessel and stir for 40min at 80 and 300r / min. Then add 2kg of dialdehyde starch, 3kg of anhydrous methanol, 1kg of boric acid, 1kg of glutaraldehyde and 2kg of 10% acetic acid solution to the reaction vessel and continue stirring for 30min to obtain the composite film-forming solution.

[0057] S5: At the wild morel mushroom growing site, dig up the top 20cm layer of undisturbed soil for cultivation. Prepare the mushroom beds at the cultivation site, then transfer the collected undisturbed soil from the wild morel growing site to the prepared mushroom beds, leveling it and applying 1.5kg / m² of soil. 2The amount of composite film-forming liquid is sprayed and compacted.

[0058] S6: Place nutrient bags on the prepared undisturbed soil for morel mushroom cultivation. The amount of nutrient bags used is 2000 kg / mu. The nutrient bags are filled with morel mushroom feed, which is obtained by mixing rice husks and wheat in a mass ratio of 1:3 and then sterilizing at high temperature. The moisture content is 45%.

[0059] S7: During cultivation, build an arched shed, control the temperature at 18℃ and the humidity at 75%, and cultivate morel mushrooms under a light flux of 700 lumens. Ventilate once every 2 days to prevent mold growth. When the diameter of the morel cap is 7cm, it can be harvested, thus completing the cultivation method using the original soil of morel mushrooms.

[0060] The polyethylene glycol used in the examples has a molecular weight of 1000.

[0061] The cultivation method using undisturbed soil provided by this invention makes full use of wild resources, preserves the microbial community and nutrients in the undisturbed soil, and can be used for the growth and reproduction of wild morel mushrooms, thereby improving the cultivation efficiency of wild morel mushrooms and protecting wild morel mushroom resources.

[0062] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0063] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A cultivation method using undisturbed soil for morel mushrooms, characterized in that, Includes the following steps: Step 1: In the wild morel mushroom growing area, dig up the top 10-20cm thick layer of original morel mushroom soil, dig a mushroom bed in the cultivation area, transfer the original morel mushroom soil to the dug mushroom bed, spread it flat, spray the composite film-forming liquid at a rate of 1-1.5kg / m2 and compact it. Step 2: Place the nutrient bags containing morel mushroom feed on the prepared soil for morel mushroom cultivation. The amount of nutrient bags used is 1500-2000 kg / mu. Step 3: During the cultivation process, build an arched shed, control the temperature at 16-18℃ and the humidity at 60-75%, and plant morel mushrooms under the condition of 500-700 lumens of light. Ventilate once every 2 days. When the diameter of the morel mushroom cap is 5-7cm, it can be harvested, thus completing the cultivation method using the original soil of morel mushrooms. The composite film-forming solution is prepared through the following steps: Amino-terminated polyethylene glycol, lignin nanoparticles, potassium humate and deionized water are added to a reaction vessel and stirred at 200-300 r / min for 30-40 min. Then, dialdehyde starch, anhydrous methanol, boric acid, glutaraldehyde and 10 wt% acetic acid solution are added and stirred for 20-30 min to obtain the composite film-forming solution. The mass ratio of the amino-terminated polyethylene glycol, lignin nanoparticles, potassium humate, deionized water, dialdehyde starch, anhydrous methanol, boric acid, glutaraldehyde, and acetic acid solution is 1:2:10:250:20:30:10:10:

20.

2. The cultivation method for morel mushrooms using undisturbed soil according to claim 1, characterized in that, The morel mushroom feed is made by mixing rice husks and wheat in a mass ratio of 1:3 and then sterilizing at high temperature. The moisture content of the morel mushroom feed is 45%.

3. The cultivation method for morel mushrooms using undisturbed soil according to claim 1, characterized in that, The lignin nanoparticles are prepared by the following steps: Ammonium lignosulfonate and deionized water were added to a reaction vessel and stirred to dissolve. Anhydrous ethanol was added dropwise under stirring at 200-300 r / min. After the addition was complete, the anhydrous ethanol was removed by rotary evaporation and then dried under vacuum to obtain lignin nanoparticles.

4. The cultivation method for morel mushrooms using undisturbed soil according to claim 3, characterized in that, The ratio of ammonium lignosulfonate, deionized water, and anhydrous ethanol is 0.1g:15mL:25-30mL.

5. The cultivation method for morel mushrooms using undisturbed soil according to claim 1, characterized in that, The amino-terminated polyethylene glycol is prepared by the following steps: Esterified polyethylene glycol and 28wt% ammonia water were added to a reaction vessel and reacted at 135-140℃ for 5-6 hours. The reaction product was extracted three times with DCM. The organic phases were combined and the pH was adjusted to 11.5-12 with 1mol / L sodium hydroxide solution. The mixture was stirred at 20-25℃ and 200-300r / min for 4-5 hours. After standing, the organic phase was separated and washed with saturated sodium chloride solution until neutral. The mixture was filtered, and the filtrate was dried with anhydrous sodium sulfate to remove water. After standing, the mixture was filtered and concentrated under reduced pressure to obtain amino-terminated polyethylene glycol.

6. The cultivation method for morel mushrooms using undisturbed soil according to claim 5, characterized in that, The mass ratio of the esterified polyethylene glycol to ammonia is 1:

4.

7. A cultivation method for morel mushrooms using undisturbed soil according to claim 5, characterized in that, The esterified polyethylene glycol is prepared by the following steps: Polyethylene glycol was dissolved in dichloromethane and transferred to a reaction vessel. Then, a 15 wt% triethylamine solution of p-toluenesulfonyl chloride was added dropwise to the reaction vessel. The reaction was kept at 20-25℃ for 10-12 h. The reaction solution was washed 2-3 times with a 3 mol / L hydrochloric acid solution. The organic phases were combined and the pH was adjusted to 7 with sodium bicarbonate. The mixture was filtered, and the filtrate was concentrated under reduced pressure to obtain esterified polyethylene glycol.

8. The cultivation method for morel mushrooms using undisturbed soil according to claim 7, characterized in that, The mass ratio of the polyethylene glycol, dichloromethane, and triethylamine solution of p-toluenesulfonyl chloride is 2:10:5-6.