Pig breeding feed composition based on bacterium-enzyme synergy and application thereof

Abstract

The invention discloses a pig breeding feed composition based on bacterium-enzyme synergy. The pig breeding feed composition comprises a piglet feed, a growing pig feed and a fattening pig feed, the piglet feed, the growing pig feed and the fattening pig feed at least comprise bacillus coagulans, clostridium butyricum, phytase, amylase, trypsin and NSP enzyme, and the bacillus coagulans can produce lactic acid and bacteriostats to enhance the intestinal barrier function of bred pigs; the clostridium butyricum can produce butyric acid and can regulate the balance of intestinal flora; phytase can decompose phytic acid and release mineral substances such as phosphorus, calcium and zinc; amylase can hydrolyze starch into soluble sugar, so that the energy utilization rate is increased; trypsin is used for degrading protein into small peptide and amino acid, is adaptive to the high-protein requirement of bred pigs, and can degrade cell wall polysaccharide of plant feed, reduce chyme viscosity and release embedded nutrients together with NSP enzyme; the invention also discloses an application of the breeding feed composition for pigs in breeding pig products.

Description

Technical Field

[0001] The invention belongs to the field of feed, and in particular relates to a pig breeding feed combination based on bacterial enzyme synergy. The invention also relates to the application of the pig breeding feed combination. Background Art

[0002] With the development of animal husbandry, feed digestibility and animal intestinal health have become key factors affecting breeding benefits. Traditional feed processing methods such as simple fermentation or enzymatic hydrolysis have certain limitations. They cannot fully degrade macromolecules in feed and lack the production of beneficial metabolites. In recent years, bacterial enzyme synergistic technology has gradually attracted attention as an emerging feed processing method. This technology can effectively improve the nutritional value and digestibility of feed by combining microbial fermentation and enzymatic hydrolysis.

[0003] Existing studies have shown that the application of bacterial enzyme synergistic technology in different feeds has significant effects. For example, Liu Lupeng et al. found that adding alkaline protease and xylanase to the diet can increase the production of reducing sugars and the efficiency of dry matter enzymatic hydrolysis. Mao Yin et al. reported that bacterial enzyme synergy (Lactobacillus plantarum and neutral protease) increased the content of crude protein, small peptides and total acid in fermented soybean meal, and the in vitro digestibility of crude protein was also improved. Zhang Yu et al. found that after replacing 10% of the basic diet with bacterial enzyme synergistic fermented feed composed of mixed bacteria (Saccharomyces cerevisiae, Bacillus subtilis, mixed lactic acid bacteria) and complex enzymes, the average daily weight gain and feed intake of piglets were improved.

[0004] For example, the patent document with publication number CN115462460B discloses a preparation method and application of a liquid bacterial enzyme synergistic feed additive for pigs, wherein sterile water is used as a carrier, a bacterial protective agent of 20 to 50% by weight of the additive is added, an enzyme protective agent of 0.1 to 0.3% by weight of the additive is added, and a stabilizer of 0.05 to 0.25% by weight of the additive is added to prepare an additive base liquid; then beneficial bacteria are added to make the beneficial bacteria concentration ≥4×10 8 CFU / mL, and finally add active enzyme to make the activity of active enzyme in the feed additive 80-1800U / L, and adjust the pH to 5-6 with phosphate buffer.

[0005] The prior art disclosed in the above patent document has opened up a new way to prepare pig feed additives. It is effectively used as an additive for pig feed, significantly increasing the feed intake and daily weight gain of live pigs, reducing the meat-to-feed ratio, improving feed utilization, and promoting the growth of fattening pigs; however, the prior art is only designed for fattening pigs, and there has been no systematic research and optimization on the selection of strains and enzymes and their optimal ratios for pigs at different growth stages. The selection of strains and enzymes lacks specificity. Most studies use a single strain or a combination of universal enzymes, and do not adapt to the digestive physiological characteristics of pigs at different growth stages (such as incomplete intestinal development of piglets). At the same time, the patent document discloses that its feed fermentation time is as long as 45 days, which to a certain extent will cause the long time of occupying the fermentation equipment, slow discharge, and difficulty in production deployment, etc., and increase production costs in various aspects. Summary of the invention

[0006] In view of the problems in the related art, the present invention proposes a pig breeding feed combination based on bacterial enzyme synergy, which can be adapted to pigs in different growth stages to overcome the above technical problems existing in the existing related art. The present invention also relates to the application of the pig breeding feed combination.

[0007] The technical solution of the present invention is achieved in this way: A pig breeding feed combination based on bacterial enzyme synergy, including piglet feed, growing pig feed and fattening pig feed; The piglet feed, growing pig feed and finishing pig feed all contain at least Bacillus coagulans, Clostridium butyricum, Bacillus subtilis, phytase, amylase, trypsin and NSP enzyme.

[0008] The Bacillus coagulans designed in the present invention can produce lactic acid and bacteriostatin, thereby enhancing the intestinal barrier function of farmed pigs; the Clostridium butyricum can produce butyric acid (the main energy source of colon epithelial cells), thereby regulating the balance of intestinal flora; the Bacillus subtilis can secrete a variety of enzymes such as protease, amylase, lipase, pectinase, glucanase, etc., thereby degrading complex organic matter in animal and plant feeds, promoting digestion and absorption, and improving feed utilization; the phytase can decompose phytic acid, releasing minerals such as phosphorus, calcium, and zinc; the amylase can hydrolyze starch into soluble sugars, thereby improving energy utilization; the trypsin can degrade proteins into small peptides and amino acids, thereby meeting the high protein needs of farmed pigs, and can degrade cell wall polysaccharides of plant feeds together with the NSP enzyme (non-starch polysaccharide enzyme), thereby reducing chyme viscosity and releasing embedded nutrients.

[0009] At the same time, the combined use of the amylase, trypsin, phytase and NSP enzyme can effectively improve the fermentation efficiency and release the nutrients in the feed more comprehensively.

[0010] Preferably, the NSP enzyme is one or a combination of xylanase and β-glucanase in any ratio.

[0011] Preferably, the piglet feed comprises a first bacterial liquid and a first enzyme liquid; The first bacterial liquid is a liquid of Bacillus coagulans: Clostridium butyricum: Bacillus subtilis in a volume ratio of 0.8-1.1:0.5-1.5:0.9-1.2; The first enzyme solution is a phytase solution: amylase solution: trypsin solution: xylanase solution: β-glucanase solution in a volume ratio of 0.8-1.2: 1.5-2.5: 0.9-1.1: 0.8-1.1: 0.9-1.2.

[0012] The pancreas of piglets before and after weaning is not fully developed, and endogenous amylase is insufficient, which cannot fully decompose the starch in the feed. At the same time, the villi of the small intestine of piglets are short, the activity of digestive enzymes is low, and the intestinal digestion capacity is weak. Undegraded starch is easy to enter the hindgut for fermentation, causing stress symptoms such as diarrhea. The present invention specifically increases the proportion of amylase, promotes the conversion of starch to glucose, and alleviates the weaning stress of piglets; the Clostridium butyricum can maintain the integrity of the intestinal mucosa, improve the digestibility of nutrients by piglets, and prevent the invasion of harmful substances and pathogens from the outside.

[0013] Preferably, the growing pig feed comprises a second bacterial liquid and a second enzyme liquid; The second bacterial liquid is a liquid of Bacillus coagulans: Clostridium butyricum: Bacillus subtilis in a volume ratio of 0.9-1.2:1.8-2.2:0.8-1.2; The second enzyme solution is a phytase solution: amylase solution: trypsin solution: xylanase solution: β-glucanase solution in a volume ratio of 0.8-1.1: 0.8-1.1: 1.5-2.5: 0.8-1.1: 0.5-1.5.

[0014] Growing pigs generally require a large amount of amino acids as raw materials to meet the needs of muscle growth. Although the gastrointestinal digestive system of growing pigs is more mature than that of the above-mentioned piglets, the secretion of endogenous trypsin is still unable to fully match the digestion needs of high-protein feeds. The present invention specifically increases the proportion of trypsin to accelerate protein decomposition, thereby meeting the muscle growth needs of growing pigs, while reducing the entry of undigested protein into the hindgut to avoid the reproduction of harmful bacteria; on the other hand, by increasing the proportion of Clostridium butyricum to regulate the flora, synergizing with trypsin can promote intestinal health and improve feed utilization.

[0015] Preferably, the fattening pig feed comprises a third bacterial liquid and a third enzyme liquid; The third bacterial liquid is a liquid of Bacillus coagulans: Clostridium butyricum: Bacillus subtilis in a volume ratio of 1.5-2.3:0.9-1.2:0.8-1.1; The volume ratio of the third enzyme solution is 0.5-1.5:0.8-1.2:0.9-1.1:1.6-2.5:0.9-1.2, which is phytase solution: amylase solution: trypsin solution: xylanase solution: β-glucanase solution.

[0016] In addition to the basic pursuit of weight gain, fattening pigs need to pay special attention to avoiding excessive fat deposition in the later stage, otherwise it will have an adverse effect on the lean meat rate. The increase in the proportion of xylanase can enhance the role of xylanase in degrading fiber, improve energy utilization, increase volatile fatty acid (VFA) energy supply, and reduce the conversion of glucose into fat in fattening pigs; at the same time, by increasing the proportion of Bacillus coagulans, it can mainly inhibit gastrointestinal pathogens and reduce diarrhea symptoms that are prone to occur during the fattening period.

[0017] Preferably, the Bacillus coagulans solution, Clostridium butyricum solution and Bacillus subtilis solution each contain 0.9×10 9 ~1.2×10 9 CFU / mL of live bacteria. Excessive live bacteria may lead to excessive consumption of nutrients such as glucose and amino acids in the feed, resulting in the final nutrient content of the feed being lower than the preset content; secondly, too many live bacteria may also interfere with the intestinal microecology of farmed pigs, which may easily cause diarrhea in farmed pigs, which is contrary to the pursuit of the present invention; finally, the present invention is based on bacterial enzyme synergistic technology, and excessive live bacteria may cause the temperature to rise too quickly during the fermentation process and exceed the preset temperature, which has an adverse effect on enzyme activity and cannot achieve the ideal bacterial enzyme synergistic effect.

[0018] Preferably, the enzyme concentration of the phytase solution is 800-1200 U / mL, the enzyme concentration of the amylase solution is 1800-2200 U / mL, the enzyme concentration of the trypsin solution is 900-1200 U / mL, the enzyme concentration of the xylanase solution is 800-1100 U / mL, and the enzyme concentration of the β-glucanase is 900-1100 U / mL. In essence, special attention should be paid to the research and development of pig feed: it not only effectively produces the effect of regulating the internal environment of farmed pigs, but also does not affect the growth, development, secretion and other activities of the organs and systems of farmed pigs themselves. Excessive enzyme intake may lead to insufficient secretion of endogenous enzymes in farmed pigs, so the enzyme concentration needs to be controlled; on the other hand, excessive enzymes may also lead to excessive enzyme activity, easily produce bitter substances, poor palatability, and easily lead to unstable temperature during fermentation.

[0019] Preferably, the Bacillus coagulans is selected from a strain with a survival rate of ≥90% at ≥80°C for 30 minutes, and has high storage stability.

[0020] Preferably, the piglet feed comprises complete piglet feed, wherein 5-15wt% of the complete piglet feed is mixed with the first bacterial liquid and the first enzyme liquid for fermentation, and 8-10L of the first bacterial liquid and 4-6L of the first enzyme liquid are added to each ton of complete piglet feed, which is set as piglet fermentation material; The growing pig feed comprises a complete feed for growing pigs, wherein 5 to 15 wt % of the complete feed for growing pigs is mixed and fermented with the second bacterial liquid and the second enzyme liquid, and 9 to 11 L of the second bacterial liquid and 5 to 6 L of the second enzyme liquid are added to each ton of the complete feed for growing pigs, which is set as the fermentation material for growing pigs; The fattening pig feed comprises a complete feed for fattening pigs, wherein 5 to 15 wt % of the complete feed for fattening pigs is mixed and fermented with the third bacterial liquid and the third enzyme liquid, and 10 to 12 L of the third bacterial liquid and 5 to 7 L of the third enzyme liquid are added to each ton of the complete feed for fattening pigs, which is set as the fermentation material for fattening pigs.

[0021] Specifically, the crude protein content of the piglet feed is designed to be 18-20%; the crude protein content of the growing pig feed is designed to be 15-17%; and the crude protein content of the fattening pig feed is designed to be 12-15%.

[0022] The present invention also discloses a fermentation method of the above-mentioned pig breeding feed combination based on bacterial enzyme synergy, and the specific operation is: the above-mentioned piglet fermentation material, growing pig fermentation material and fattening pig fermentation material are respectively loaded into fermentation tanks, the temperature is set to 36-38°C, taking into account the growth of bacteria and enzyme activity, the humidity is 55-65%, to prevent the material from agglomerating and ensure that the bacterial enzyme can effectively diffuse, the closed fermentation is carried out for 45-50 hours, the fermentation efficiency and nutrient retention are balanced, and the fermentation is stirred every 10-15 hours to promote uniform fermentation.

[0023] Compared with the prior art, the present invention has higher fermentation efficiency and shorter fermentation time by selecting and designing the proportions of bacteria, enzymes and other materials, which effectively improves production efficiency and is more convenient and flexible for actual production deployment. At the same time, the fermentation method disclosed in the present invention is streamlined, which is conducive to technology scale-up and mass production.

[0024] The complete feed of the present invention is a basic daily diet prepared according to a conventional formula, and the farm can prepare it by itself or purchase commercially available finished products. The complete feed generally includes bulk raw materials such as corn and soybean meal, and preferably the bulk raw materials have a particle size of ≤60 mesh or the bulk raw materials are crushed to a particle size of ≤60 mesh.

[0025] Most of the existing technologies are based on the idea of ​​fermenting the complete feed of the daily diet of pigs with bacterial liquid and enzyme liquid. The fermentation volume of this method is extremely large, resulting in a long fermentation time. The larger the fermentation tank, the greater the production cost and the floor space required, which is not conducive to small-scale farms to conduct self-tests and control costs. The present invention, on the other hand, takes 10wt% of the daily diet and ferments it with bacterial enzymes. The fermentation of a small amount of material is obviously more flexible, and it is mixed with the premix after fermentation, which can also more accurately control the proportion of daily nutrients. Finally, if the fermentation fails unexpectedly, the loss is relatively low.

[0026] The invention also discloses the application of the above-mentioned pig breeding feed combination based on bacterial enzyme synergy in pig breeding feed.

[0027] The present invention optimizes pig feed based on the bacteria-enzyme synergistic technology. According to the differences in the physiological needs of pigs at different growth stages, the selection, ratio and process parameters of bacteria and enzymes are optimized in stages to improve the digestibility and utilization of feed, promote the intestinal health of farmed pigs, and thus significantly improve the nutritional value of feed and the health level of pigs, providing an efficient and stable feed solution for large-scale farming. DETAILED DESCRIPTION

[0028] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0029] Example 1 A pig breeding feed combination based on bacterial enzyme synergy, including piglet feed, growing pig feed and fattening pig feed. The preparation process is as follows: (1) Preparation of bacterial culture A. Culture medium for Bacillus coagulans (CAS No. 68038-65-3, survival rate at 80℃>90%): 20 g / L glucose, 10 g / L peptone, 5 g / L yeast powder and KH 2 PO 4 2g / L, pH 6.8.

[0030] B. Culture medium for Clostridium butyricum (ATCC19398): soluble starch 15 g / L, tryptone 10 g / L, L-cysteine ​​hydrochloride 0.5 g / L, pH 7.0.

[0031] C. Culture medium for Bacillus subtilis (CAS No. 68038-70-0): 30 g / L corn flour, 20 g / L soybean meal, (NH 4 ) 2 SO 43g / L, pH 7.2.

[0032] Culture conditions: Culture at 37°C for 24 h, collect the cells by centrifugation, and adjust the viable cell count to 1×10 9 CFU / mL, and Bacillus coagulans liquid, Clostridium butyricum liquid and Bacillus subtilis liquid were prepared.

[0033] (2) Preparation of enzyme solution The following enzymes are all commercial grade enzyme preparations (purity ≥ 90%).

[0034] Dissolution method: Mix various enzymes in proportion and dissolve in pH 6.5 phosphate buffer. The final concentration is adjusted as follows: The enzyme concentration of the phytase solution was 1000 U / mL, the enzyme concentration of the amylase solution was 2000 U / mL, the enzyme concentration of the trypsin solution was 1000 U / mL, the enzyme concentration of the xylanase solution was 1000 U / mL, and the enzyme concentration of the β-glucanase was 1000 U / mL.

[0035] (3) Configure materials Formulate complete feed for piglets, complete feed for growing pigs and complete feed for fattening pigs.

[0036] The total weight of the complete feed required for feeding the piglets is estimated, and the complete feed is weighed accordingly. 10wt% of the complete feed is mixed with the first bacterial liquid and the first enzyme liquid for fermentation. For every ton of the complete feed, 9L of the first bacterial liquid and 6L of the first enzyme liquid are added to prepare the fermentation material for the piglets; The first bacterial liquid consists of 3L of Bacillus coagulans liquid, 3L of Clostridium butyricum liquid and 3L of Bacillus subtilis liquid; The first enzyme solution consists of 1L of phytase solution, 2L of amylase solution, 1L of trypsin solution, 1L of xylanase solution and 1L of β-glucanase solution.

[0037] The total weight of the complete feed required for feeding growing pigs is estimated, and the complete feed is weighed accordingly. 10 wt% of the complete feed is mixed with the second bacterial liquid and the second enzyme liquid for fermentation. For every ton of the complete feed, 10 L of the second bacterial liquid and 6 L of the second enzyme liquid are added to prepare a fermentation material for growing pigs. The second bacterial liquid is composed of 2.5L of Bacillus coagulans liquid, 5L of Clostridium butyricum liquid and 2.5L of Bacillus subtilis liquid; The second enzyme solution consists of 1L of phytase solution, 1L of amylase solution, 2L of trypsin solution, 1L of xylanase solution and 1L of β-glucanase solution.

[0038] The total weight of the complete feed required for feeding fattening pigs is estimated, and the complete feed is weighed accordingly. 10wt% of the complete feed is mixed with the third bacterial liquid and the third enzyme liquid for fermentation. For every ton of the complete feed, 10L of the third bacterial liquid and 6L of the third enzyme liquid are added to prepare a fermented material for fattening pigs. The third bacterial liquid is composed of 5L of Bacillus coagulans liquid, 2.5L of Clostridium butyricum liquid and 2.5L of Bacillus subtilis liquid; The third enzyme solution consists of 1L of phytase solution, 1L of amylase solution, 1L of trypsin solution, 2L of xylanase solution and 1L of β-glucanase solution.

[0039] (4) Fermentation The above-mentioned piglet fermentation material, growing pig fermentation material and fattening pig fermentation material were respectively loaded into different fermentation tanks, set the temperature to 37°C, the humidity to 60%, and ferment for 48 hours in a closed manner. According to the working hours of the farm, stir the material every 10 to 15 hours.

[0040] The pH value of the sample was 3.9, indicating that the fermentation was mature.

[0041] (5) The fermented piglet material after fermentation is mixed evenly with the remaining 90wt% of complete piglet feed to prepare a piglet feed with a crude protein content of 18.52%; The fermented growing pig fermented material is then mixed evenly with the remaining 90wt% of the growing pig complete feed to prepare a growing pig feed with a crude protein content of 16.32%; The fermented material for fattening pigs that has completed fermentation is then mixed evenly with the remaining 90wt% of complete feed for fattening pigs to prepare a fattening pig feed with a crude protein content of 14.71%.

[0042] Comparative Example 1 Compared with Example 1, this comparative example does not contain amylase and trypsin; the remaining operations are the same.

[0043] Similarly, the fermentation was closed for 48 hours and the pH value of the sample was 4.8, which means that the fermentation was not mature.

[0044] Comparative Example 2 Compared with Example 1, the Bacillus coagulans solution, Clostridium butyricum solution and Bacillus subtilis solution of this comparative example respectively contain 1.5×10 9 The number of viable bacteria in CFU / mL; The enzyme concentration of the phytase solution is 1500 U / mL, the enzyme concentration of the amylase solution is 2500 U / mL, the enzyme concentration of the trypsin solution is 1500 U / mL, the enzyme concentration of the xylanase solution is 1200 U / mL, and the enzyme concentration of the β-glucanase is 1200 U / mL; the remaining operations are the same.

[0045] When the material was stirred every 10 to 15 hours, it exceeded 38°C, and the fermentation temperature was too high. Similarly, after 48 hours of closed fermentation, the pH value of the sample was 2.8, and the acidity was too low.

[0046] Comparative Example 3 A pig breeding feed combination comprises the complete feed for piglets, the complete feed for growing pigs and the complete feed for fattening pigs prepared in Example 1, that is, does not contain bacteria, enzymes and related designs.

[0047] The following experimental pigs were provided by a pig farm in Guangzhou, and all of them were lean Large White pigs.

[0048] (1) Piglet test 240 28-day-old weaned piglets were selected and randomly divided into two groups, each group containing 120 piglets, and were fed with the piglet feed of Example 1 and Comparative Example 3, respectively, for a test period of 42 days.

[0049] (2) Growing pig test 180 growing pigs with a body weight of approximately 45-50 kg were selected and randomly divided into two groups, each group containing 90 growing pigs, and were fed with the growing pig feed of Example 1 and Comparative Example 3, respectively, for a test period of 60 days.

[0050] (3) 60 fattening pigs with a body weight of 75-80 kg were selected and randomly divided into two groups, each group containing 30 fattening pigs, and fed with the fattening pig feed of Example 1 and Comparative Example 3, respectively. The test period was 30 days.

[0051] The experimental pigs in the experimental period were fed three times a day in a "feeding + free access to food + drinking water" mode. The breeding site was kept clean and well ventilated.

[0052] A. Piglet test records 1) Record the initial and final weights of the piglets at the beginning and end of the trial period, and then calculate the average daily weight gain data.

[0053] Average daily gain (ADG) = (average final weight - average initial weight) / number of days of experiment 2) Observe every day whether any piglets have diarrhea symptoms (i.e., porridge-like or watery feces), record the number of piglets with diarrhea and the number of days, and calculate the diarrhea rate according to the following formula.

[0054] At the end of the experiment, compared with the piglets fed with comparative example 3, the average daily weight gain of the piglets fed with example 1 increased by 21.17%, the diarrhea rate decreased by 16.3%, and the number of intestinal lactic acid bacteria increased by 3 times.

[0055] B. Growing Pig Test Records 1) Record the initial and final weights of growing pigs at the beginning and end of the trial period, and then calculate the daily weight gain data.

[0056] Average daily gain (ADG) = (average final weight - average initial weight) / number of days of experiment 2) The initial and final backfat thickness of growing pigs at the beginning and end of the trial period were recorded by B-type ultrasonic scanner, and the average value was calculated to calculate the backfat growth rate.

[0057] Compared with the growing pigs fed with Comparative Example 3, the average daily weight gain of the growing pigs fed with Example 1 increased by 7.15%, and the back fat thickness increased by 6%.

[0058] C. Fattening Pig Experiment Record 1) Record the initial weight, final weight and feed intake of fattening pigs at the beginning and end of the experimental period, and then calculate the average daily weight gain and feed-to-meat ratio.

[0059] Average daily gain (ADG) = (final weight - initial weight) / number of days of experiment Feed to meat ratio = average daily feed intake / average daily weight gain 2) The back fat thickness of the fattening pigs at the end of the test period was recorded by a B-type ultrasonic scanner to observe whether the present invention can control the back fat thickness of the fattening pigs to a certain extent, thereby improving the lean meat rate.

[0060] 3) Blood samples were taken from fattening pigs on the 25th day of the trial period to measure serum immune antibody IgG.

[0061] Compared with the fattening pigs fed with Comparative Example 3, the fattening pigs fed with Example 1 had a higher feed-to-meat ratio, that is, a higher feed conversion rate, a 6.3% reduction in backfat thickness, and an 18% increase in serum IgG levels.

[0062] According to the disclosure and teaching of the above specification, those skilled in the art to which the present invention belongs can also change and modify the above implementation mode. Therefore, the present invention is not limited to the specific implementation modes disclosed and described above, and some modifications and changes to the present invention should also fall within the protection scope of the claims of the present invention. In addition, although some specific terms are used in this specification, these terms are only for the convenience of description and do not constitute any limitation to the present invention.

Claims

1. A pig breeding feed combination based on bacterial enzyme synergy, characterized in that: Includes piglet feed, grower pig feed and fattening pig feed; The piglet feed, growing pig feed and finishing pig feed all contain at least Bacillus coagulans, Clostridium butyricum, phytase, amylase, trypsin and NSP enzyme.

2. The pig breeding feed combination based on bacterial enzyme synergy according to claim 1, characterized in that: The piglet feed, growing pig feed and finishing pig feed all further comprise Bacillus subtilis.

3. The pig breeding feed combination based on bacterial enzyme synergy according to claim 1 or 2, characterized in that: The NSP enzyme is one of xylanase and β-glucanase or a combination of the two in any ratio.

4. The pig breeding feed combination based on bacterial enzyme synergy according to claim 3 is characterized in that: The piglet feed comprises a first bacterial liquid and a first enzyme liquid, wherein the first bacterial liquid is a Bacillus coagulans liquid: Clostridium butyricum liquid: Bacillus subtilis liquid in a volume ratio of 0.8-1.1:0.5-1.5:0.9-1.2, and the first enzyme liquid is a phytase liquid: amylase liquid: trypsin liquid: xylanase liquid: β-glucanase liquid in a volume ratio of 0.8-1.2:1.5-2.5:0.9-1.1:0.8-1.1:0.9-1.2; The growing pig feed comprises a second bacterial liquid and a second enzyme liquid, wherein the second bacterial liquid is a Bacillus coagulans liquid: Clostridium butyricum liquid: Bacillus subtilis liquid in a volume ratio of 0.9-1.2:1.8-2.2:0.8-1.2, and the second enzyme liquid is a phytase liquid: amylase liquid: trypsin liquid: xylanase liquid: β-glucanase liquid in a volume ratio of 0.8-1.1:0.8-1.1:1.5-2.5:0.8-1.1:0.5-1.5; The fattening pig feed comprises a third bacterial liquid and a third enzyme liquid, wherein the third bacterial liquid is a Bacillus coagulans liquid: Clostridium butyricum liquid: Bacillus subtilis liquid in a volume ratio of 1.5-2.3:0.9-1.2:0.8-1.1, and the third enzyme liquid is a phytase liquid: amylase liquid: trypsin liquid: xylanase liquid: β-glucanase liquid in a volume ratio of 0.5-1.5:0.8-1.2:0.9-1.1:1.6-2.5:0.9-1.

2.

5. The pig breeding feed combination based on bacterial enzyme synergy according to claim 4, characterized in that: The Bacillus coagulans solution, Clostridium butyricum solution and Bacillus subtilis solution respectively contain 0.9×10 9 ~1.2×10 9 The number of viable bacteria in CFU / mL; The enzyme concentration of the phytase solution is 800-1200 U / mL; The enzyme concentration of the amylase solution is 1800-2200 U / mL; The enzyme concentration of the trypsin solution is 900-1200 U / mL; The enzyme concentration of the xylanase solution is 800-1100 U / mL; The enzyme concentration of the β-glucanase is 900-1100 U / mL.

6. The pig breeding feed combination based on bacterial enzyme synergy according to claim 5, characterized in that: The piglet feed comprises a complete piglet feed, wherein 5 to 15 wt % of the complete piglet feed is mixed with the first bacterial liquid and the first enzyme liquid for fermentation, and 8 to 10 L of the first bacterial liquid and 4 to 6 L of the first enzyme liquid are added to each ton of the complete piglet feed, which is set as the piglet fermentation material; The growing pig feed comprises a complete feed for growing pigs, wherein 5 to 15 wt % of the complete feed for growing pigs is mixed and fermented with the second bacterial liquid and the second enzyme liquid, and 9 to 11 L of the second bacterial liquid and 5 to 6 L of the second enzyme liquid are added to each ton of the complete feed for growing pigs, which is set as the fermentation material for growing pigs; The fattening pig feed comprises a complete feed for fattening pigs, wherein 5 to 15 wt % of the complete feed for fattening pigs is mixed and fermented with the third bacterial liquid and the third enzyme liquid, and 10 to 12 L of the third bacterial liquid and 5 to 7 L of the third enzyme liquid are added to each ton of the complete feed for fattening pigs, which is set as the fermentation material for fattening pigs.

7. The pig breeding feed combination based on bacterial enzyme synergy according to claim 6, characterized in that: The crude protein content of the piglet feed is designed to be 18-20%; The crude protein content of the growing pig feed is designed to be 15-17%; The crude protein content of the fattening pig feed is designed to be 12-15%.

8. The pig breeding feed combination based on bacterial enzyme synergy according to claim 6, characterized in that: The specific operation of the fermentation is: respectively put the fermented materials of piglets, growing pigs and fattening pigs into fermentation tanks, set the temperature to 35-39°C and the humidity to 55-65%, and ferment for 45-50 hours in a closed manner, stirring every 10-15 hours.

9. The pig breeding feed combination based on bacterial enzyme synergy according to claim 1, characterized in that: The piglets refer to farmed pigs from weaning to 30 kg, the growing pigs refer to farmed pigs from 30 to 60 kg, and the fattening pigs refer to pigs from 60 kg to market.

10. Use of the pig breeding feed combination based on bacterial enzyme synergy as claimed in any one of claims 1 to 9 in pig breeding products.

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