Acanthopanax extract powder with excellent water solubility and taste and preparation method of acanthopanax extract powder

Abstract

The invention provides acanthopanax extract powder with excellent water solubility and taste and a preparation method of the acanthopanax extract powder, and belongs to the technical field of health care product or food preparation. The radix acanthopanacis senticosi extract powder is prepared from the following raw materials: radix acanthopanacis senticosi extract, medium chain triglyceride, a surfactant, a cosurfactant and cyclodextrin, and the mass ratio of the radix acanthopanacis senticosi extract to the medium chain triglyceride to the surfactant to the cosurfactant is (0.10 to 0.13) to (0.20 to 0.26) to (0.21 to 0.42) to (0.18 to 0.49). The ratio of the total mass of the acanthopanax extract, the medium chain triglyceride, the surfactant and the cosurfactant to the mass of the cyclodextrin is (1.5-2.5): 1, the preparation method of the acanthopanax extract powder is simple, the water solubility and bioavailability of the acanthopanax extract are remarkably improved, the application requirements of health care products and functional foods are met, and the acanthopanax extract powder is suitable for industrial production. And the transportation, storage and use convenience of the product is improved.

Description

Technical Field

[0001] The invention belongs to the technical field of health care products or food preparation, and in particular relates to an acanthopanax senticosus extract powder with excellent water solubility and taste and a preparation method thereof. Background Art

[0002] Acanthopanax senticosus is a traditional Chinese medicinal material with multiple bioactive ingredients, which is widely used in health products and functional foods such as enhancing immunity, relieving fatigue and anti-oxidation. Acanthopanax senticosus extract can be obtained by extracting the active ingredients in Acanthopanax senticosus. However, due to the high viscosity and poor water solubility of Acanthopanax senticosus extract, it is inconvenient to use it directly as a finished product raw material. These shortcomings not only affect the processing and transportation of Acanthopanax senticosus extract, but also limit its application in various dosage forms. In addition, Acanthopanax senticosus itself has a strong flavor, which affects its taste and consumer acceptance.

[0003] In order to solve the shortcomings of Acanthopanax senticosus extract in terms of water solubility, flavor, stability, etc., the prior art uses solid dispersion, effervescent disintegration and other technologies to prepare it into various preparation forms. For example, patent CN1733033A discloses an Acanthopanax dripping pill and a preparation method thereof, which uses solid dispersion technology to mix Acanthopanax senticosus extract with a matrix such as poloxamer and sodium carboxymethyl starch to prepare dripping pills, but the Acanthopanax dripping pills prepared by this method do not involve how to improve the flavor of Acanthopanax senticosus and are basically not water-soluble. Patent CN101744852A discloses a method for preparing Acanthopanax effervescent tablets, but this technology has limited effects on the formation of powders and the improvement of taste.

[0004] It can be seen from this that in the prior art, the products of Acanthopanax senticosus extract generally have the problems of poor water solubility, low bioavailability and strong flavor, which not only affect its application in health products and functional foods, but also cause inconvenience in transportation, storage and use of products. In addition, due to reasons such as preparation technology, most oral preparations of Acanthopanax senticosus extract have the problems of long dissolution time limit, low dissolution, poor absorbability, low hepato-intestinal first-pass effect and low bioavailability after taking, thereby affecting the performance of drug efficacy, and also directly affecting the therapeutic effect. In addition, prior art such as conventional oral preparations, usually also has the problem of complex process and high production cost.

[0005] To this end, the present invention provides an acanthopanax senticosus extract powder with excellent water solubility and mouthfeel and a preparation method thereof. Summary of the invention

[0006] In order to solve the above technical problems, the present invention proposes an Acanthopanax senticosus extract powder with excellent water solubility and taste and a preparation method thereof, which can effectively mask the unique flavor and improve the taste of Acanthopanax senticosus extract by using a cyclodextrin inclusion composite self-emulsifying system while improving the water solubility and bioavailability of Acanthopanax senticosus extract, and powderize the extract, thereby greatly improving the portability, stability and applicability of the product. The preparation method of the present invention is simple and provides a new technical path for the multi-field application of Acanthopanax senticosus extract.

[0007] To achieve the above object, the present invention provides the following technical solutions:

[0008] One of the technical solutions proposed by the present invention:

[0009] A powder of acanthopanax senticosus extract with excellent water solubility and mouthfeel. The raw materials include acanthopanax senticosus extract, medium-chain triglycerides, surfactants, cosurfactants and cyclodextrin. The mass ratio of the acanthopanax senticosus extract, medium-chain triglycerides (MCT), surfactants and cosurfactants is (0.10-0.13): (0.20-0.26): (0.21-0.42): (0.18-0.49), and the mass ratio of the total mass of the acanthopanax senticosus extract, medium-chain triglycerides, surfactants and cosurfactants to the cyclodextrin is (1.5-2.5): 1.

[0010] Furthermore, the surfactant includes Tween 80 and / or Span 80, preferably Tween 80.

[0011] Furthermore, the co-surfactant includes propylene glycol and / or isopropanol, preferably propylene glycol.

[0012] The present invention uses medium chain triglyceride (MCT) as an oil phase solvent, and controls the concentration of Acanthopanax senticosus extract within the range of 10-20% (w / v) to ensure that it is fully dissolved and has a good emulsification effect. By limiting the mass ratio of Acanthopanax senticosus extract, medium chain triglyceride, surfactant, and co-surfactant, the emulsion is realized to quickly form uniform and stable emulsion droplets within 5 seconds, and the emulsion droplet diameter is controlled at 300-500nm, which is conducive to subsequent inclusion and drying treatment. And by limiting the amount of cyclodextrin, the water solubility of Acanthopanax senticosus extract is effectively improved, and a suitable powder particle structure is provided.

[0013] The second technical solution of the present invention:

[0014] A method for preparing the Acanthopanax senticosus extract powder having excellent water solubility and taste comprises the following steps:

[0015] (1) dissolving Acanthopanax senticosus extract in medium chain triglycerides to obtain an oil phase;

[0016] (2) adding a surfactant and a co-surfactant to the oil phase in sequence, and mixing to obtain an emulsion;

[0017] (3) adding cyclodextrin to the emulsion and stirring evenly to obtain a cyclodextrin inclusion emulsion;

[0018] (4) The cyclodextrin inclusion emulsion is pre-frozen and then freeze-dried to obtain the Acanthopanax senticosus extract powder with excellent water solubility and mouthfeel.

[0019] Furthermore, in step (4), the pre-freezing treatment is carried out at a temperature of -50 to -40°C and for a period of 1 to 3 hours.

[0020] Furthermore, in step (4), the freeze-drying temperature is -40 to -20°C, the vacuum degree is less than 0.1 Pa, and the time is 48-72 hours. Under these conditions, the powder can be ensured to have good resolubility.

[0021] Furthermore, in step (2), the droplet diameter of the emulsion is 300-500 nm.

[0022] Furthermore, in step (4), before pre-freezing the cyclodextrin inclusion emulsion, a step of adding a solid agent is also included; further, the solid agent includes microcrystalline cellulose.

[0023] The present invention selects medium-chain triglycerides (MCT) as an oil phase solvent to prepare the oil phase system of Acanthopanax senticosus extract, and determines the types and concentrations of surfactants and co-surfactants through solubility experiments, so that the oil phase and the surfactants and co-surfactants achieve good compatibility. Preferably, the surfactants include Tween 80 and Span 80, and the co-surfactants include propylene glycol and isopropanol. The ratio of the oil phase to the surfactant and the co-surfactant is further determined to ensure the emulsification effect, emulsification equilibrium time and uniformity of droplet distribution of the system.

[0024] Adding an appropriate amount of cyclodextrin to the emulsified oil phase system, cyclodextrin can enhance its water solubility and stability by encapsulating the active ingredients in Acanthopanax senticosus extract, while effectively masking the strong flavor of Acanthopanax senticosus extract. By adjusting the amount of cyclodextrin and the conditions of its encapsulation process, the solubility and taste of the final powder product can be ensured.

[0025] Solid agents such as microcrystalline cellulose are added to the cyclodextrin inclusion system to help form a powder with good fluidity and dispersibility. The mixed system is spray-dried or freeze-dried to powderize it, and an Acanthopanax senticosus extract powder that is easy to pack and has high stability is obtained, which realizes the efficient powderization of Acanthopanax senticosus extract, significantly improves its water solubility and bioavailability, and successfully masks its strong flavor, thereby improving the product's portability and consumer acceptance.

[0026] Compared with the prior art, the present invention has the following advantages and technical effects:

[0027] The preparation method of the Acanthopanax senticosus extract powder of the present invention significantly improves the water solubility, bioavailability and flavor masking effect of the Acanthopanax senticosus extract, meets the application requirements of health products and functional foods, and improves the convenience of transportation, storage and use of the product. Specifically:

[0028] (1) Improved water solubility

[0029] The present invention significantly improves the water solubility of Acanthopanax senticosus extract by introducing a cyclodextrin composite self-emulsifying system. Experimental data show that after the Acanthopanax senticosus extract powder is dispersed in water, a uniform solution is formed, and the dissolution time is shortened to within about 5 seconds, which is significantly better than the prior art, making it more suitable for fast brewing and drinking or fast release of active ingredients.

[0030] (2) Enhanced bioavailability

[0031] The present invention adopts cyclodextrin to encapsulate active ingredients, effectively increasing the solubility and stability of the active ingredients in Acanthopanax senticosus extract, thereby improving its absorption efficiency in the body. Animal experiments have verified that compared with traditional Acanthopanax senticosus extract preparations, the Acanthopanax senticosus extract powder prepared by the present invention has an in vivo bioavailability increased by about 25-40%, which has a significant contribution to enhancing the health care effect of the Acanthopanax senticosus active ingredients.

[0032] (3) Flavor masking and increased consumer acceptance

[0033] Cyclodextrin inclusion complex can effectively mask the strong taste of Acanthopanax senticosus, making the powder taste milder and satisfying consumers' flavor preferences. According to a blind consumer test, about 85% of the subjects said that the powder product has a significant improvement in taste compared to traditional Acanthopanax senticosus products and is more acceptable.

[0034] (4) Improved convenience and preservation

[0035] The powder form of the Acanthopanax senticosus extract of the present invention is convenient for packaging, transportation and storage, and solves the disadvantage that the Acanthopanax senticosus extract is prone to deterioration in a liquid state. Accelerated aging tests have shown that the powder remains stable at room temperature for 6 months without obvious changes in color or taste, and has a long shelf life, thereby providing economic benefits for industrial applications. BRIEF DESCRIPTION OF THE DRAWINGS

[0036] The accompanying drawings constituting a part of the present invention are used to provide a further understanding of the present invention. The exemplary embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute an improper limitation of the present invention. In the accompanying drawings:

[0037] Figure 1 The figure is a schematic flow chart of a method for preparing the Acanthopanax senticosus extract powder having excellent water solubility and taste in Example 1 of the present invention;

[0038] Figure 2 UPLC chromatograms of a 5-fold diluted mixture reference substance (A) and the Acanthopanax senticosus extract powder sample prepared in Example 1 (B). DETAILED DESCRIPTION

[0039] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as limiting the present invention, but should be understood as a more detailed description of certain aspects, features, and embodiments of the present invention.

[0040] It should be understood that the terms described in the present invention are only for describing special embodiments and are not intended to limit the present invention. In addition, for the numerical range in the present invention, it should be understood that each intermediate value between the upper and lower limits of the scope is also specifically disclosed. Each smaller range between the intermediate value in any stated value or stated range and any other stated value or intermediate value in the described range is also included in the present invention. The upper and lower limits of these smaller ranges can be independently included or excluded in the scope.

[0041] Unless otherwise indicated, all technical and scientific terms used herein have the same meanings as those generally understood by those skilled in the art. Although the present invention describes only preferred methods and materials, any methods and materials similar or equivalent to those described herein may also be used in the implementation or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials associated with the documents. In the event of a conflict with any incorporated document, the content of this specification shall prevail.

[0042] It will be apparent to those skilled in the art that various modifications and variations may be made to the specific embodiments of the present invention description without departing from the scope or spirit of the present invention. Other embodiments derived from the present invention description will be apparent to those skilled in the art. The present invention description and examples are exemplary only.

[0043] The words “include,” “including,” “have,” “contain,” etc. used in this document are open-ended terms, meaning including but not limited to.

[0044] The embodiment of the present invention provides an Acanthopanax senticosus extract powder with excellent water solubility and taste. The raw materials include Acanthopanax senticosus extract, medium-chain triglycerides, surfactants, co-surfactants and cyclodextrin. The mass ratio of the Acanthopanax senticosus extract, medium-chain triglycerides (MCT), surfactants and co-surfactants is (0.10-0.13): (0.20-0.26): (0.21-0.42): (0.18-0.49), and the mass ratio of the total mass of the Acanthopanax senticosus extract, medium-chain triglycerides, surfactants and co-surfactants to the cyclodextrin is (1.5-2.5): 1.

[0045] In a preferred embodiment of the present invention, the surfactant comprises Tween 80 and / or Span 80, preferably Tween 80.

[0046] In a preferred embodiment of the present invention, the co-surfactant includes propylene glycol and / or isopropanol, preferably propylene glycol.

[0047] The embodiment of the present invention also proposes a method for preparing the Acanthopanax senticosus extract powder having excellent water solubility and taste, comprising the following steps:

[0048] (1) dissolving Acanthopanax senticosus extract in medium chain triglycerides to obtain an oil phase;

[0049] (2) adding a surfactant and a co-surfactant to the oil phase in sequence, and mixing to obtain an emulsion;

[0050] (3) adding cyclodextrin to the emulsion and stirring evenly to obtain a cyclodextrin inclusion emulsion;

[0051] (4) The cyclodextrin inclusion emulsion is pre-frozen and then freeze-dried to obtain the Acanthopanax senticosus extract powder with excellent water solubility and mouthfeel.

[0052] In step (4) of the preferred embodiment of the present invention, the pre-freezing treatment is performed at a temperature of -50 to -40°C for 1 to 3 hours.

[0053] In step (4) of the preferred embodiment of the present invention, the freeze-drying temperature is -40 to -20°C, the vacuum degree is less than 0.1 Pa, and the time is 48-72 hours. Under these conditions, the powder is ensured to have good resolubility.

[0054] In step (2) of a preferred embodiment of the present invention, the droplet diameter of the emulsion is 300-500 nm.

[0055] In step (4) of the preferred embodiment of the present invention, before the cyclodextrin inclusion emulsion is pre-frozen, a step of adding a solid agent is also included; further, the solid agent includes microcrystalline cellulose; the amount of the solid agent added is.

[0056] All raw materials used in the examples of the present invention are commercially available.

[0057] The technical solution of the present invention is further illustrated by the following embodiments.

[0058] Example 1

[0059] A method for preparing a powdered acanthopanax extract with excellent water solubility and taste, the schematic diagram of the process is shown in Figure 1 , specifically including the following steps:

[0060] (1) dissolving Acanthopanax senticosus extract in MCT to obtain an oil phase;

[0061] (2) adding Tween 80 and propylene glycol to the oil phase obtained in step (1) in sequence, and mixing to obtain an emulsion with a droplet diameter of 300 nm, wherein the mass ratio of Acanthopanax senticosus extract, MCT, Tween 80 and propylene glycol in the above process is 1:2:2.1:4.9;

[0062] (3) adding cyclodextrin to the prepared emulsion at a mass ratio of 1:1.5 (i.e., the mass ratio of cyclodextrin to emulsion is 1:1.5), and stirring to obtain a cyclodextrin inclusion emulsion;

[0063] (4) Add microcrystalline cellulose (the amount added is 1% of the system mass) to the obtained cyclodextrin inclusion emulsion, and then pre-freeze it (temperature is -50°C, time is 3 hours), and then freeze-dry it (temperature is -40°C, vacuum degree is less than 0.1Pa, time is 48 hours) to obtain an Acanthopanax senticosus extract powder with excellent water solubility and taste.

[0064] Example 2

[0065] A method for preparing an Acanthopanax senticosus extract powder having excellent water solubility and taste, specifically comprising the following steps:

[0066] (1) dissolving Acanthopanax senticosus extract in MCT to obtain an oil phase;

[0067] (2) adding Tween 80 and propylene glycol to the oil phase obtained in step (1) in sequence, and mixing to obtain an emulsion with a droplet diameter of 350 nm, wherein the mass ratio of Acanthopanax senticosus extract, MCT, Tween 80 and propylene glycol in the above process is 1.3:2.6:4.2:1.8;

[0068] (3) adding cyclodextrin to the prepared emulsion at a mass ratio of 1:2.0 (i.e., the mass ratio of cyclodextrin to emulsion is 1:2.0), and stirring to obtain a cyclodextrin inclusion emulsion;

[0069] (4) Add microcrystalline cellulose (the amount added is 1% of the system mass) to the obtained cyclodextrin inclusion emulsion, and then pre-freeze it (temperature is -50°C, time is 3 hours), and then freeze-dry it (temperature is -40°C, vacuum degree is less than 0.1Pa, time is 48 hours) to obtain an Acanthopanax senticosus extract powder with excellent water solubility and taste.

[0070] Example 3

[0071] A method for preparing an Acanthopanax senticosus extract powder having excellent water solubility and taste, specifically comprising the following steps:

[0072] (1) dissolving Acanthopanax senticosus extract in MCT to obtain an oil phase;

[0073] (2) adding Tween 80 and propylene glycol to the oil phase obtained in step (1) in sequence, and mixing to obtain an emulsion with a droplet diameter of 350 nm, wherein the mass ratio of Acanthopanax senticosus extract, MCT, Tween 80 and propylene glycol in the above process is 1.1:2.2:3.6:3.1;

[0074] (3) adding cyclodextrin to the prepared emulsion at a mass ratio of 1:2.5 (i.e., the mass ratio of cyclodextrin to emulsion is 1:2.0), and stirring to obtain a cyclodextrin inclusion emulsion;

[0075] (4) Add microcrystalline cellulose (the amount added is 1% of the system mass) to the obtained cyclodextrin inclusion emulsion, and then pre-freeze it (temperature is -50°C, time is 3 hours), and then freeze-dry it (temperature is -40°C, vacuum degree is less than 0.1Pa, time is 48 hours) to obtain an Acanthopanax senticosus extract powder with excellent water solubility and taste.

[0076] Comparative Example 1

[0077] Raw material: Acanthopanax senticosus extract.

[0078] Performance Testing

[0079] (1) Water solubility test

[0080] The Acanthopanax extract powder prepared in Example 1 was dissolved in pure water at 20°C. It was tested that it could be completely dissolved within 5 seconds, and formed a clear liquid after re-dissolution without precipitation. The solubility was increased to 100 mg / mL, which was 82% higher than that of the untreated Acanthopanax extract (Comparative Example 1).

[0081] The Acanthopanax extract powder prepared in Example 2 was dissolved in 20°C pure water. It was tested that it could be completely dissolved within 5 seconds, and formed a clear liquid after re-dissolution without precipitation. The solubility was increased to 105 mg / mL, which was 91% higher than that of the untreated Acanthopanax extract (Comparative Example 1).

[0082] The Acanthopanax extract powder prepared in Example 3 was dissolved in 20°C pure water. It was tested that it could be completely dissolved within 5 seconds, and formed a clear liquid after re-dissolution without precipitation. The solubility was increased to 94 mg / mL, which was 70% higher than that of the untreated Acanthopanax extract (Comparative Example 1).

[0083] (2) Bioavailability

[0084] Take 10 rats and randomly divide them into two groups. After 5 days of adaptive feeding, fast overnight but not water for 12 hours. The next day, Example 1 and Comparative Example 1 were respectively given to rats by gavage at 600 mg / kg. The two groups were respectively blooded from the tail of each rat at 9 time periods (5min, 30min, 1.0h, 1.5h, 2.0h, 4.0h, 8.0h, 12.0h, 24.0h) after administration. The blood samples were placed in heparin sodium tubes, centrifuged at 4000r / min for 15min, and the upper plasma was separated and sealed in a -20°C refrigerator. Thawed in a 37°C water bath before measurement. The blood drug concentration of each group was detected by high performance liquid chromatography, and the pharmacokinetic parameters of each group of rats were statistically analyzed. The main pharmacokinetic parameters of CRA in plasma after administration of each group are shown in Table 1.

[0085] Table 1 Pharmacokinetic parameters of the main active substances in the plasma of the two groups

[0086]

[0087] From the data analysis of Table 1, it can be seen that the absorption degree of the Acanthopanax senticosus extract powder group of Example 1 is significantly higher than that of the Acanthopanax senticosus extract group of Comparative Example 1, and the relative bioavailability of the Acanthopanax senticosus extract powder of Example 1 is increased by 25-40%. The experimental results show that the preparation method of the Acanthopanax senticosus extract powder of Example 1 significantly enhances the absorption efficiency of the active ingredients in the organism.

[0088] (3) Sensory test

[0089] 50 consumers were recruited, and the products of Example 1 and Comparative Example 1 were randomly distributed. The consumers were required to taste and fill in an evaluation form in turn. The evaluation indicators included taste, flavor, appearance, overall preference (1-5 points) and subjective acceptance. The consumers were asked to rinse their mouths after tasting to avoid cross-interference. After collecting the results, it was found that the Acanthopanax senticosus extract powder product of Example 1 had a mild flavor, and the unique strong flavor of Acanthopanax senticosus was significantly masked. The average values ​​of the five evaluation indicators of taste, flavor, appearance and overall preference were all higher than those of the control example, and the acceptance of the testers reached more than 85%.

[0090] (4) Convenience and preservation

[0091] The powder form is convenient for packaging, transportation and storage, which solves the disadvantage that Acanthopanax senticosus extract is prone to deterioration in liquid state. According to the accelerated aging test (high temperature 40℃±2℃, high humidity 75%±5%RH), the powder remains stable at room temperature for 6 months without obvious color or taste changes, and has a long shelf life.

[0092] (5) Determination of the contents of five active ingredients in Acanthopanax senticosus extract powder by UPLC

[0093] The ultra-high performance liquid chromatograph was Agilent 1290 Infinity II, and the chromatographic conditions were: Agilent Eclipse Plus C18 RRHD column, 1.8 μm, 2.1 × 50 mm; H 2 O(A)-CH 3 OH(B) was used as the mobile phase, and gradient elution was performed according to Table 2; the detection wavelength was 220 nm; the column temperature was 20°C; and the flow rate was 0.2 mL / min.

[0094] Accurately weigh appropriate amounts of syringin, chlorogenic acid, eleutheroside E, isofraxidin and rutin reference substances, add 50% (volume concentration) methanol to prepare a reference substance mixed solution containing 125 μg syringin, 225 μg chlorogenic acid, 75 μg eleutheroside, 30 μg isofraxidin and 25 μg rutin per 1 mL, and dilute 2.5 times, 5 times, 10 times and 25 times to prepare 5 groups of mixed standard solutions with different concentrations.

[0095] Table 2 UPLC elution gradient

[0096] Time (min) A％ B％ 0～4 100→80 0→20 4～14 80→50 30→50 14～30 50→0 50→100

[0097] Under the above analytical method conditions, 5 mixed standard solutions of different concentrations were taken for UPLC analysis, and the peak area (Y) was linearly regressed against the concentration (X) (average value of 3 measurements) to obtain the regression equation and correlation coefficient. The results are shown in Table 3.

[0098] Table 3 Concentration, regression equation, and correlation coefficient results

[0099]

[0100] Take 0.5 g of the Acanthopanax senticosus extract powder sample prepared in Example 1, accurately weigh it, place it in a stoppered conical flask, accurately add 10 mL of 50% methanol, weigh it, ultrasonically treat it for 30 minutes, take it out, cool it, make up the weight with 50% methanol, shake it well, and perform UPLC analysis on the Acanthopanax senticosus extract powder sample. The samples were injected three times in parallel. According to the regression equation, the percentage content of each active substance in the powder sample was shown in Table 4.

[0101] Table 4 Content of each active substance in powder samples

[0102] Compound Peak area Concentration (μg / mL) Percentage Syringin 350.64354 26.28 0.5256％ Chlorogenic acid 318.265784 45.52 0.9104％ Acanthopanax glycoside E 197.684669 16.53 0.3306％ Isotriazine 112.689368 6.17 0.1234％ Rutin 37.18379 5.15 0.1030％

[0103] The UPLC chromatograms of the 5-fold diluted mixture reference substance (A) and the Acanthopanax senticosus extract powder sample (B) prepared in Example 1 are shown in FIG. Figure 2 In the figure, 1, 2, 3, 4, and 5 represent syringin, chlorogenic acid, eleutheroside E, isoflurane, and rutin, respectively.

[0104] Depend on Figure 2 It can be seen from Tables 3 and 4 that the retention times of syringin, chlorogenic acid, eleutheroside E, isofraxidin and rutin in the Acanthopanax senticosus extract powder sample prepared in Example 1 are 6.66 min, 8.38 min, 12.04 min, 14.76 min and 18.28 min, respectively, which are consistent with the retention times of the corresponding substances in the mixed standard solution reference substance, indicating that the preparation process of the present invention has no effect on the five active ingredients.

[0105] In summary, the present invention effectively solves the deficiencies of Acanthopanax senticosus extract in terms of water solubility, bioavailability, flavor and powderization by optimizing the emulsification system and cyclodextrin inclusion technology, making its application prospect in the field of functional foods broader.

[0106] The above are only preferred specific embodiments of the present invention, but the protection scope of the present invention is not limited thereto. Any changes or substitutions that can be easily thought of by a person skilled in the art within the technical scope disclosed by the present invention should be included in the protection scope of the present invention. Therefore, the protection scope of the present invention should be based on the protection scope of the claims.

Claims

1. A Acanthopanax senticosus extract powder having excellent water solubility and taste, characterized in that: The raw materials include acanthopanax senticosus extract, medium-chain triglycerides, surfactants, co-surfactants and cyclodextrin. The mass ratio of the acanthopanax senticosus extract, medium-chain triglycerides, surfactants and co-surfactants is (0.10-0.13): (0.20-0.26): (0.21-0.42): (0.18-0.49). The mass ratio of the total mass of the acanthopanax senticosus extract, medium-chain triglycerides, surfactants and co-surfactants to the cyclodextrin is (1.5-2.5):

1.

2. The Acanthopanax senticosus extract powder with excellent water solubility and mouthfeel according to claim 1, characterized in that: The surfactant includes Tween 80 and / or Span 80.

3. The Acanthopanax senticosus extract powder with excellent water solubility and mouthfeel according to claim 1, characterized in that: The co-surfactant includes propylene glycol and / or isopropyl alcohol.

4. A method for preparing the Acanthopanax senticosus extract powder having excellent water solubility and taste according to any one of claims 1 to 3, characterized in that: The following steps are involved: (1) dissolving Acanthopanax senticosus extract in medium chain triglycerides to obtain an oil phase; (2) adding a surfactant and a co-surfactant to the oil phase in sequence, and mixing to obtain an emulsion; (3) adding cyclodextrin to the emulsion and stirring evenly to obtain a cyclodextrin inclusion emulsion; (4) The cyclodextrin inclusion emulsion is pre-frozen and then freeze-dried to obtain the Acanthopanax senticosus extract powder with excellent water solubility and mouthfeel.

5. The method for preparing the Acanthopanax senticosus extract powder having excellent water solubility and mouthfeel according to claim 4, characterized in that: In step (4), the pre-freezing treatment is carried out at a temperature of -50 to -40°C and for a period of 1 to 3 hours.

6. The method for preparing the Acanthopanax senticosus extract powder having excellent water solubility and mouthfeel according to claim 4, characterized in that: In step (4), the freeze-drying temperature is -40 to -20°C, the vacuum degree is <0.1 Pa, and the time is 48-72 hours.

7. The method for preparing the Acanthopanax senticosus extract powder having excellent water solubility and mouthfeel according to claim 4, characterized in that: In step (2), the droplet diameter of the emulsion is 300-500nm.

8. The method for preparing the Acanthopanax senticosus extract powder having excellent water solubility and mouthfeel according to claim 4, characterized in that: In step (4), before the cyclodextrin inclusion emulsion is pre-frozen, a step of adding a solid agent is also included.

9. The method for preparing the Acanthopanax senticosus extract powder having excellent water solubility and mouthfeel according to claim 8, characterized in that: The solid agent includes microcrystalline cellulose.

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