Freckle-fading and whitening emulsion containing various plant extracts and preparation method of spot-fading and whitening emulsion

Abstract

The invention discloses spot-fading and whitening emulsion containing various plant extracts and a preparation method of the spot-fading and whitening emulsion, and belongs to the technical field of spot-fading and whitening cosmetics. The invention discloses an anti-allergy skin care product, which is prepared from the following ingredients in percentage by weight: 3.2 to 4 percent of hexyldecanol, 2 to 3 percent of nicotinamide, 1 to 1.5 percent of tranexamic acid, 0.5 to 1 percent of 4-butylresorcinol, 0.5 to 1 percent of hydrolyzed yeast protein, 0.3 to 0.8 percent of undecylenoyl phenylalanine, 0.1 to 0.5 percent of rhizoma bletillae extracts, 1.5 to 2 percent of radix caulophylli extracts, 0.1 to 0.5 percent of snow lotus herb extracts, 2 to 5 percent of humectants, 2.2 to 3.5 percent of thickening agents and the balance of deionized water. 1.5-2% of an emulsifier, 1-1.5% of a filler, and the balance of water. The composite emulsion system contains the eleutheroside B and other components, the polyethylene glycol assists in dispersing the arbutin, and all the components cooperate with one another, so that the purposes of efficiently fading spots and whitening can be achieved. The rhizoma bletillae, the radix caulophylli and the saussurea involucrata extract are compounded, polysaccharide and phlegm of the rhizoma bletillae extract have the effects of protecting and softening the skin, various compounds of the radix caulophylli extract and components of the saussurea involucrata extract act together, all-around spot fading and whitening can be achieved, and the spot fading and whitening mask is suitable for different skin types.

Description

Technical Field

[0001] The invention belongs to the technical field of spot-lightening and whitening cosmetics, and in particular relates to a spot-lightening and whitening emulsion containing multiple plant extracts and a preparation method thereof. Background Art

[0002] In today's society, with the rapid improvement of people's living standards and the continuous evolution of aesthetic concepts, the demand for skin care and whitening has become more refined and diversified. Fair and translucent skin is not only related to external beauty, but also a direct reflection of healthy skin condition, so it has become the core demand of many consumers in terms of skin care. There are a wide variety of whitening products on the market, and various whitening ingredients are emerging in an endless stream, but most products still have many limitations. Traditional whitening agents such as hydroquinone, although the whitening effect is immediate, have side effects that cannot be ignored. Long-term use can easily cause skin allergies, aggravated pigmentation, and may even cause irreversible skin damage. For safety considerations, it has gradually faded out of the ranks of mainstream skin care raw materials.

[0003] As one of the most popular whitening ingredients, arbutin plays a key blocking role at the source of melanin production by virtue of its ability to effectively inhibit the activity of tyrosinase, thus showing a considerable skin whitening effect, and is highly favored in the field of cosmetics research and development. However, the physical and chemical properties of arbutin itself expose obvious shortcomings in skin care products in the form of emulsions. It has poor dispersibility in the emulsion system, and it is difficult to disperse evenly and stably in the water phase and the oil phase. It is very easy to agglomerate and precipitate; this not only affects the appearance and texture of the product, but more importantly, it hinders its transdermal absorption efficiency. In addition, skin care products with a single whitening ingredient can often only focus on a certain link in the production of melanin, and it is difficult to deal with the complex causes of pigmentation in an all-round way. The formation of pigmentation is the result of the interweaving of internal and external factors. Factors such as ultraviolet invasion, endocrine disorders, skin inflammation, and skin aging can induce or aggravate pigmentation problems. Therefore, developing a skin care product that can not only overcome the problem of ingredient dispersion and improve transdermal absorption, but also comprehensively consider the causes of pigmentation and achieve multi-dimensional whitening and spot-lightening effects has become a technical difficulty and R&D hotspot that the current cosmetics industry urgently needs to overcome. Summary of the invention

[0004] In view of the above situation, in order to overcome the defects of the prior art, the present invention uses a composite emulsion system and multiple plant extracts to prepare an emulsion with whitening and spot-lightening effects.

[0005] In order to achieve the above-mentioned purpose, the following technical scheme is adopted: the present invention provides a freckle-lightening and whitening lotion containing multiple plant extracts, which comprises the following components in percentage by weight: 3.2-4% of hexyldecanol, 2-3% of niacinamide, 1-1.5% of tranexamic acid, 0.5-1% of 4-butylresorcinol, 0.5-1% of hydrolyzed yeast protein, 0.3-0.8% of undecylenoylphenylalanine, 0.1-0.5% of bletilla striata extract, 1.5-2% of ophiopogon erythrorhizon extract, 0.1-0.5% of snow lotus extract, 2-5% of moisturizer, 2.2-3.5% of thickener, 1.5-2% of emulsifier and 1-1.5% of filler, and the rest is water.

[0006] Furthermore, the spot-lightening and whitening emulsion intermediate is prepared by the following steps:

[0007] S1, mixing Acanthopanax senticosus saponin B, polyethylene glycol, 1,2-hexanediol and methyl glucose sesquistearate, and heating until each component is dissolved to obtain a first solution;

[0008] S2. Disperse arbutin and isopropyl myristate and add them to the first solution. Mix well and cool to room temperature to obtain a second solution. Then, slowly add deionized water to the second solution at a stirring speed of 500-800 rpm at room temperature and stir well to obtain the spot-lightening and whitening emulsion intermediate.

[0009] Furthermore, the Bletilla striata extract is prepared by the following steps: drying the Bletilla striata raw material until the moisture is less than 15%, coarsely crushing it to 20 mesh, and then crushing it to 60-120 mesh with an ultrafine grinder to obtain Bletilla striata powder, adding pure water to the Bletilla striata powder in a mass ratio of Bletilla striata: pure water = 1:20-1:25, stirring at room temperature at 30-40rpm, then leaching for 12h, heating to 88-92°C, stirring and extracting for 3h, cooling to room temperature, centrifuging at 10°C and 4000rpm for 30min, collecting the supernatant, transferring it to a single-effect concentrator, and concentrating it at 60-65°C to a specific gravity of 1.14-1.16 to obtain a Bletilla striata extract concentrate, continuously adding the Bletilla striata water extraction supernatant concentrate to anhydrous ethanol, stirring evenly at 60-80rpm to a final ethanol mass concentration of 70-72%, standing for 2h, removing the supernatant and centrifuging at 1400rpm for 15min, and freeze-drying the resulting precipitate to obtain the Bletilla striata extract.

[0010] Bletilla striata contains abundant polysaccharides, which have good film-forming properties. When applied to the skin, it can form a protective film on the skin surface, reduce the loss of skin moisture, and play a moisturizing role. This film can also resist the stimulation of the external environment on the skin, such as preventing ultraviolet rays, pollutants, etc. from damaging the skin. Bletilla striata also contains volatile oils, mucus and other ingredients. Mucus can increase the softness of the skin and make the skin feel smoother and more delicate.

[0011] Furthermore, the Herba Lycopersicon esculentum extract is prepared by the following steps: steaming the rhizome of Herba Lycopersicon esculentum at 80-100°C, then drying at 40-60°C for 12-24h, mechanically crushing, and then adding 95% ethanol with a volume of 1-3 times the weight of Herba Lycopersicon esculentum for three extractions, combining the extracts, concentrating under reduced pressure to obtain Herba Lycopersicon esculentum concentrate, adding 1-3 times the volume of deionized water of the Herba Lycopersicon esculentum concentrate to form a suspension, and then extracting with ethyl acetate and n-butanol, concentrating the ethyl acetate extraction part and the n-butanol extraction part, purifying by silica gel column chromatography, removing the solvent under reduced pressure and combining to obtain the Herba Lycopersicon esculentum extract.

[0012] The extract of Herba Lycopersicon contains a variety of saponin chemicals, cyclopentane compounds and sesquiterpenoid compounds. Saponin chemicals can inhibit the activity of tyrosinase and have anti-inflammatory effects. They have a certain alleviating effect on skin inflammation, such as skin redness and swelling caused by acne and blackheads. It can inhibit the production of inflammatory factors, reduce inflammatory reactions, and help the skin restore to a healthy state. The extract of Herba Lycopersicon also has antioxidant properties. The active ingredients in it can remove free radicals in the skin, delay the aging process of the skin, and maintain the elasticity and luster of the skin. Cyclopentane compounds have strong antioxidant capacity, eliminate the stimulation of free radicals on tyrosinase, and significantly reduce the production of melanin. Sesquiterpenoid compounds can promote metabolism, accelerate skin cell renewal, and accelerate the shedding of keratinocytes containing melanin.

[0013] Furthermore, the snow lotus extract is prepared by the following steps: washing, drying, and crushing the snow lotus, adding 3-5 times the weight of 80% methanol to perform three extractions, centrifuging the extract, and then concentrating to obtain a snow lotus concentrate, mixing it with 3-5 times the volume of petroleum ether of the snow lotus concentrate, taking the water phase, mixing the water phase with n-butanol for extraction, concentrating the n-butanol phase, and then separating it with a macroporous resin to obtain a purified solution, and concentrating the purified solution under reduced pressure, and drying it to obtain the snow lotus extract.

[0014] Snow lotus contains flavonoids, which have good antioxidant and antibacterial effects. They can protect skin cells from free radicals, inhibit the activity of tyrosinase, reduce the synthesis of melanin, and prevent the transfer of melanocytes to the stratum corneum of the skin, thereby reducing the formation of spots and achieving the effect of whitening and lightening the skin to prevent premature aging of the skin, such as wrinkles and sagging. Its antibacterial effect helps inhibit the growth of harmful bacteria on the skin surface, prevent and improve skin problems such as acne. Snow lotus extract also contains alkaloids and other ingredients, which can regulate the microcirculation of the skin, ensure that skin cells obtain adequate nutrition and oxygen, keep the skin healthy, and enhance skin metabolism.

[0015] Furthermore, in step S1, the weight ratio of Acanthopanax senticosus saponin B, polyethylene glycol, 1,2-hexanediol and methyl glucose sesquistearate is 15-22:25-35:4-5:10-12.

[0016] Furthermore, in step S2, the weight ratio of arbutin, isopropyl myristate, the first solution and deionized water is 10-20:5-15:40-60:30-50.

[0017] Furthermore, the silica gel column chromatography purification of the ethyl acetate extraction part is carried out in a system of petroleum ether:ethyl acetate:methanol=7:2:3, and the silica gel column chromatography purification of the n-butanol extraction part is carried out in a system of dichloromethane:ethanol=1:1.

[0018] Furthermore, the moisturizing agent is one or more of tocopherol acetate, glycerol, propylene glycol, butylene glycol, 1,2-pentanediol, betaine, mannose, trehalose and glycerol polymethacrylate.

[0019] Furthermore, the thickener is one or more of carbomer, xanthan gum, sclerotium gum, laureth-7, beheneth-25, hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, vinylformamide / ammonium acryloyldimethyl taurate copolymer, polysorbate-20, polysorbate-40 and polysorbate-60.

[0020] Furthermore, the emulsifier is one or more of sorbitan isostearate, methyl glucose dioleate, polyglyceryl-3 methyl glucose distearate, ethoxylated methyl glucose distearate, cetearyl alcohol polyoxyethylene ether-20, and isostearyl alcohol polyether-25.

[0021] Furthermore, the filler is one or more of silica, nano-sized zinc oxide, nano-sized titanium dioxide, nano-sized aluminum oxide, nano-sized boron nitride, polydimethylsiloxane, cyclopolydimethylsiloxane and polymethyl methacrylate.

[0022] On the other hand, the present invention also provides a method for preparing a spot-lightening and whitening emulsion containing multiple plant extracts, comprising the following steps:

[0023] (1) mixing the humectant, thickener, filler and water uniformly, heating to 75-85° C., homogenizing and keeping the temperature for 20-30 minutes to obtain a first mixture;

[0024] (2) adding an emulsifier, hexyldecyl alcohol, niacinamide, tranexamic acid, 4-butylresorcinol, hydrolyzed yeast protein and undecylenoylphenylalanine to the first mixture and stirring to obtain a second mixture;

[0025] (3) The second mixture is cooled to 40° C., and then the Bletilla striata extract, the Herba Lycopodii extract and the Herba Saussureae extract are added, stirred evenly, and then cooled to room temperature to obtain the spot-lightening and whitening emulsion.

[0026] The beneficial effects of the present invention are:

[0027] (1) The composite emulsion system of the present invention comprises Acanthopanax senticosus saponin B, polyethylene glycol, 1,2-hexanediol, methyl glucose sesquistearate, isopropyl myristate and arbutin, wherein polyethylene glycol helps to evenly disperse arbutin, 1,2-hexanediol maintains the stability of the system, methyl glucose sesquistearate reduces interfacial tension and prevents arbutin from agglomerating, and isopropyl myristate enhances skin permeability, wherein arbutin can fully inhibit tyrosinase activity, reduce melanin production, lighten existing spots, and prevent the appearance of new spots, thereby efficiently achieving skin lightening and whitening;

[0028] (2) The present invention scientifically compounds Bletilla striata extract, Herba Lycopersicon esculentum extract and Herba Saussureae extract, which has an ideal spot-lightening and whitening effect. The polysaccharides in the Bletilla striata extract can form a protective film on the skin surface, reduce water loss and resist external stimuli; the mucilage makes the skin feel softer and more delicate; the saponin compounds in the Herba Lycopersicon esculentum extract inhibit tyrosinase activity, have anti-inflammatory and detumescent effects; the cycloterpenoid compounds are antioxidant and reduce melanin production; the sesquiterpenoid compounds accelerate metabolism and promote the shedding of keratinocytes containing melanin; the flavonoid compounds in the Herba Saussureae extract are antioxidant and antibacterial, reduce melanin synthesis and transport, and inhibit the growth of harmful bacteria; the alkaloids regulate skin microcirculation and ensure cell nutrition supply. The three work together to achieve all-round spot-lightening and whitening effects and improve skin health, so the present invention is suitable for people with different skin types. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 The results of the tyrosinase activity inhibition test of a spot-lightening and whitening lotion containing multiple plant extracts of the present invention;

[0030] Figure 2 The free radical scavenging test results of the spot-lightening and whitening lotion containing multiple plant extracts of the present invention are shown.

[0031] The accompanying drawings are used to provide further understanding of the present invention and constitute a part of the specification. They are used to explain the present invention together with the embodiments of the present invention and do not constitute a limitation of the present invention. DETAILED DESCRIPTION

[0032] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments in the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0033] Unless otherwise defined, all professional and scientific terms used herein have the same meanings as those familiar to those skilled in the art. In addition, any methods and materials similar or equivalent to those described herein may be applied to the present invention. The preferred implementation methods and materials described herein are for demonstration purposes only and are not intended to limit the content of this application.

[0034] The experimental methods in the following examples are conventional methods unless otherwise specified, and the experimental materials used in the following examples are purchased from commercial channels unless otherwise specified.

[0035] Example 1

[0036] A freckle-lightening and whitening lotion containing multiple plant extracts comprises the following percentages of components by weight: 4% hexyldecyl alcohol, 3% niacinamide, 1.5% tranexamic acid, 1% 4-butylresorcinol, 1% hydrolyzed yeast protein, 0.8% undecylenoyl phenylalanine, 0.5% bletilla striata extract, 2% ophiopogon erythrorhizon extract, 0.5% snow lotus extract, 5% moisturizer, 3.5% thickener, 2% emulsifier and 1.5% filler, and the rest is water.

[0037] The spot-lightening and whitening emulsion intermediate is prepared by the following steps:

[0038] S1, mixing Acanthopanax senticosus saponin B, polyethylene glycol, 1,2-hexanediol and methyl glucose sesquistearate in a weight ratio of 22:35:5:12, and heating until each component is dissolved to obtain a first solution;

[0039] S2. Disperse arbutin and isopropyl myristate and add them to the first solution. After mixing evenly, cool to room temperature to obtain a second solution. Then, slowly add deionized water to the second solution at a stirring speed of 800 rpm at room temperature and stir evenly to obtain the spot-lightening and whitening emulsion intermediate, wherein the weight ratio of arbutin, isopropyl myristate, the first solution and deionized water is 15:60:50.

[0040] The Bletilla striata extract is prepared by the following steps: The Bletilla striata extract is prepared by the following steps: Drying the Bletilla striata raw material until the moisture content is less than 15%, coarsely crushing it to 20 mesh, and then crushing it to 6 mesh with an ultrafine grinder to obtain Bletilla striata powder, adding the Bletilla striata powder to pure water in a mass ratio of Bletilla striata: pure water = 1:20, stirring at 30rpm at room temperature, then leaching for 12h, heating to 88°C, stirring and extracting for 3h, cooling to room temperature, centrifuging at 10°C and 4000rpm for 30min, collecting the supernatant, transferring it to a single-effect concentrator, and concentrating it at 60°C to a specific gravity of 1.14 to obtain a Bletilla striata extract concentrate, continuously adding the Bletilla striata water extraction supernatant concentrate to anhydrous ethanol, stirring evenly at 60rpm until the final ethanol mass concentration is 70%, standing for 2h, removing the supernatant and centrifuging at 1400rpm for 15min, and freeze-drying the obtained precipitate to obtain the Bletilla striata extract.

[0041] The extract of Herba Lycopersicon esculentum is prepared by the following steps: the rhizome of Herba Lycopersicon esculentum is steamed at 100°C, then dried at 60°C for 24 hours, mechanically crushed, and then 3 times the weight of Herba Lycopersicon esculentum is added with 95% ethanol for extraction three times, the extracts are combined, and concentrated under reduced pressure to obtain a Herba Lycopersicon esculentum concentrate, 3 times the volume of deionized water of the Herba Lycopersicon esculentum concentrate is added to form a suspension, and then extracted with ethyl acetate and n-butanol, the ethyl acetate extraction part is purified by silica gel column chromatography in a system of petroleum ether: ethyl acetate: methanol = 7:2:3, and the n-butanol extraction part is purified by silica gel column chromatography in a system of dichloromethane: ethanol = 1:1, the solvent is removed under reduced pressure and combined to obtain the Herba Lycopersicon esculentum extract.

[0042] The snow lotus extract is prepared by the following steps: washing, drying and crushing the snow lotus, adding 5 times the weight of 80% methanol to perform three extractions, centrifuging the extract and then concentrating it to obtain a snow lotus concentrate, mixing it with 5 times the volume of petroleum ether of the snow lotus concentrate, taking the water phase, mixing the water phase with n-butanol for extraction, concentrating the n-butanol phase, and separating it with a macroporous resin to obtain a purified solution, concentrating the purified solution under reduced pressure, and drying it to obtain the snow lotus extract.

[0043] The moisturizer is selected from glycerin and butylene glycol, and the composition is composed of a mass ratio of 3:2; the thickener is selected from carbomer and xanthan gum, and the composition is composed of a mass ratio of 1:1; the emulsifier is selected from sorbitan isostearate and cetearyl alcohol polyoxyethylene ether-20, and the composition is composed of a mass ratio of 2:3; the filler is selected from silica and polydimethylsiloxane, and the composition is composed of a mass ratio of 4:1.

[0044] A method for preparing a spot-lightening and whitening emulsion containing multiple plant extracts comprises the following steps:

[0045] (1) mixing a humectant, a thickener, a filler and water uniformly, heating to 85° C., homogenizing and keeping the temperature for 30 minutes to obtain a first mixture;

[0046] (2) adding an emulsifier, hexyldecyl alcohol, niacinamide, tranexamic acid, 4-butylresorcinol, hydrolyzed yeast protein and undecylenoylphenylalanine to the first mixture and stirring to obtain a second mixture;

[0047] (3) The second mixture is cooled to 40° C., and then the Bletilla striata extract, the Herba Lycopodii extract and the Herba Saussureae extract are added, stirred evenly, and then cooled to room temperature to obtain the spot-lightening and whitening emulsion.

[0048] Example 2

[0049] A freckle-lightening and whitening lotion containing multiple plant extracts comprises the following components in percentage by weight: 3.2% of hexyldecyl alcohol, 2% of niacinamide, 1% of tranexamic acid, 0.5% of 4-butylresorcinol, 0.5% of hydrolyzed yeast protein, 0.3% of undecylenoylphenylalanine, 0.1% of bletilla striata extract, 1.5% of ophiopogon erythrorhizon extract, 0.1% of snow lotus extract, 2% of moisturizing agent, 2.2% of thickener, 1.5% of emulsifier and 1% of filler, and the rest is water.

[0050] The spot-lightening and whitening emulsion intermediate is prepared by the following steps:

[0051] S1, mixing Acanthopanax senticosus saponin B, polyethylene glycol, 1,2-hexanediol and methyl glucose sesquistearate in a weight ratio of 15:25:4:10, and heating until each component is dissolved to obtain a first solution;

[0052] S2. Disperse arbutin and isopropyl myristate and add them to the first solution. After mixing evenly, cool to room temperature to obtain a second solution. Then, slowly add deionized water to the second solution at a stirring speed of 500 rpm at room temperature and stir evenly to obtain the spot-lightening and whitening emulsion intermediate, wherein the weight ratio of arbutin, isopropyl myristate, the first solution and deionized water is 10:5:40:30.

[0053] The Bletilla striata extract is prepared by the following steps: The Bletilla striata extract is prepared by the following steps: Drying the Bletilla striata raw material until the moisture is less than 15%, coarsely crushing it to 20 mesh, and then crushing it to 120 mesh with an ultrafine grinder to obtain Bletilla striata powder, adding the Bletilla striata powder to pure water in a mass ratio of Bletilla striata: pure water = 1:25, stirring at 40rpm at room temperature, then leaching for 12h, heating to 92°C, stirring and extracting for 3h, cooling to room temperature, centrifuging at 10°C and 4000rpm for 30min, collecting the supernatant, transferring it to a single-effect concentrator, and concentrating it at 65°C to a specific gravity of 1.16 to obtain a Bletilla striata extract concentrate, continuously adding the Bletilla striata water extraction supernatant concentrate to anhydrous ethanol, stirring evenly at 80rpm to a final ethanol mass concentration of 72%, standing for 2h, removing the supernatant and centrifuging at 1400rpm for 15min, and freeze-drying the obtained precipitate to obtain the Bletilla striata extract.

[0054] The extract of Herba Lycopersicon esculentum is prepared by the following steps: the rhizome of Herba Lycopersicon esculentum is steamed at 80°C, then dried at 40°C for 12 hours, mechanically crushed, then leached three times with 95% ethanol (1 times the weight of Herba Lycopersicon esculentum), the extracts are combined, and concentrated under reduced pressure to obtain a Herba Lycopersicon esculentum concentrate, deionized water (1 times the volume of the Herba Lycopersicon esculentum concentrate) is added to form a suspension, and then extracted with ethyl acetate and n-butanol, the ethyl acetate extraction part is purified by silica gel column chromatography in a system of petroleum ether: ethyl acetate: methanol = 7:2:3, the n-butanol extraction part is purified by silica gel column chromatography in a system of dichloromethane: ethanol = 1:1, the solvent is removed under reduced pressure and combined to obtain the Herba Lycopersicon esculentum extract.

[0055] The snow lotus extract is prepared by the following steps: washing, drying and crushing the snow lotus, adding 3 times the weight of 80% methanol to perform three extractions, centrifuging the extract and then concentrating it to obtain a snow lotus concentrate, mixing it with 3 times the volume of petroleum ether of the snow lotus concentrate, taking the water phase, mixing the water phase with n-butanol for extraction, concentrating the n-butanol phase, and separating it with a macroporous resin to obtain a purified solution, concentrating the purified solution under reduced pressure, and drying it to obtain the snow lotus extract.

[0056] The moisturizer is selected from tocopherol acetate, betaine, and mannose, and is composed in a mass ratio of 1:2:1; the thickener is selected from sclerotium gum and hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, and is composed in a mass ratio of 3:2; the emulsifier is selected from methyl glucose dioleate and polyglycerol-3 methyl glucose distearate, and is composed in a mass ratio of 1:1; the filler is selected from nano-zinc dioxide and nano-titanium dioxide, and is composed in a mass ratio of 1:1.

[0057] A method for preparing a spot-lightening and whitening emulsion containing multiple plant extracts comprises the following steps:

[0058] (1) mixing the humectant, thickener, filler and water uniformly, heating to 75° C., homogenizing and keeping the temperature for 20 minutes to obtain a first mixture;

[0059] (2) adding an emulsifier, hexyldecyl alcohol, niacinamide, tranexamic acid, 4-butylresorcinol, hydrolyzed yeast protein and undecylenoylphenylalanine to the first mixture and stirring to obtain a second mixture;

[0060] (3) The second mixture is cooled to 40° C., and then the Bletilla striata extract, the Herba Lycopodii extract and the Herba Saussureae extract are added, stirred evenly, and then cooled to room temperature to obtain the spot-lightening and whitening emulsion.

[0061] Example 3

[0062] A freckle-lightening and whitening lotion containing multiple plant extracts comprises the following percentages of components by weight: 3.6% of hexyldecyl alcohol, 2.5% of niacinamide, 1.2% of tranexamic acid, 0.7% of 4-butylresorcinol, 0.7% of hydrolyzed yeast protein, 0.5% of undecylenoylphenylalanine, 0.3% of bletilla striata extract, 1.7% of ophiopogon erythrorhizon extract, 0.3% of snow lotus extract, 3% of moisturizing agent, 2.8% of thickener, 1.7% of emulsifier and 1.2% of filler, and the rest is water.

[0063] The spot-lightening and whitening emulsion intermediate is prepared by the following steps:

[0064] S1, mixing Acanthopanax senticosus saponin B, polyethylene glycol, 1,2-hexanediol and methyl glucose sesquistearate in a weight ratio of 18:30:4.5:11, and heating until each component is dissolved to obtain a first solution;

[0065] S2. Disperse arbutin and isopropyl myristate and add them to the first solution. After mixing evenly, cool to room temperature to obtain a second solution. Then, slowly add deionized water to the second solution at a stirring speed of 600 rpm at room temperature and stir evenly to obtain the spot-lightening and whitening emulsion intermediate, wherein the weight ratio of arbutin, isopropyl myristate, the first solution and deionized water is 15:10:50:40.

[0066] The Bletilla striata extract is prepared by the following steps: The Bletilla striata extract is prepared by the following steps: Drying the Bletilla striata raw material until the moisture content is less than 15%, coarsely crushing it to 20 mesh, and then crushing it to 100 mesh with an ultrafine grinder to obtain Bletilla striata powder, adding the Bletilla striata powder to pure water in a mass ratio of Bletilla striata: pure water = 1:22, stirring at 35rpm at room temperature, then leaching for 12h, heating to 90°C, stirring and extracting for 3h, cooling to room temperature, centrifuging at 10°C and 4000rpm for 30min, collecting the supernatant, transferring it to a single-effect concentrator, and concentrating it at 62°C to a specific gravity of 1.15 to obtain a Bletilla striata extract concentrate, continuously adding the Bletilla striata water extraction supernatant concentrate to anhydrous ethanol, stirring evenly at 70rpm to a final ethanol mass concentration of 70%, standing for 2h, removing the supernatant and centrifuging at 1400rpm for 15min, and freeze-drying the obtained precipitate to obtain the Bletilla striata extract.

[0067] The extract of Herba Lycopersicon esculentum is prepared by the following steps: the rhizome of Herba Lycopersicon esculentum is steamed at 90°C, then dried at 50°C for 18 hours, mechanically crushed, then leached three times with 95% ethanol twice the weight of Herba Lycopersicon esculentum, the extracts are combined, and concentrated under reduced pressure to obtain a Herba Lycopersicon esculentum concentrate, deionized water twice the volume of the Herba Lycopersicon esculentum concentrate is added to form a suspension, and then extracted with ethyl acetate and n-butanol, the ethyl acetate extraction part is purified by silica gel column chromatography in a system of petroleum ether: ethyl acetate: methanol = 7:2:3, the n-butanol extraction part is purified by silica gel column chromatography in a system of dichloromethane: ethanol = 1:1, the solvent is removed under reduced pressure and combined to obtain the Herba Lycopersicon esculentum extract.

[0068] The snow lotus extract is prepared by the following steps: washing, drying and crushing the snow lotus, adding 4 times the weight of 80% methanol to perform three extractions, centrifuging the extract and then concentrating it to obtain a snow lotus concentrate, mixing it with 4 times the volume of petroleum ether of the snow lotus concentrate, taking the water phase, mixing the water phase with n-butanol for extraction, concentrating the n-butanol phase, and separating it with a macroporous resin to obtain a purified solution, concentrating the purified solution under reduced pressure, and drying it to obtain the snow lotus extract.

[0069] The moisturizer is selected from propylene glycol, 1,2-pentanediol, and trehalose, and is composed in a mass ratio of 2:1:2; the thickener is selected from laureth-7, beheneth-25, and polysorbate 20, and is composed in a mass ratio of 1:1:1; the emulsifier is selected from ethoxylated methyl glucose distearate and isosteareth-25, and is composed in a mass ratio of 2:3; the filler is selected from nano-alumina, nano-boron nitride, and polymethyl methacrylate, and is composed in a mass ratio of 1:1:2.

[0070] A method for preparing a spot-lightening and whitening emulsion containing multiple plant extracts comprises the following steps:

[0071] (1) mixing a humectant, a thickener, a filler and water uniformly, heating to 80° C., homogenizing and keeping the temperature for 25 minutes to obtain a first mixture;

[0072] (2) adding an emulsifier, hexyldecyl alcohol, niacinamide, tranexamic acid, 4-butylresorcinol, hydrolyzed yeast protein and undecylenoylphenylalanine to the first mixture and stirring to obtain a second mixture;

[0073] (3) The second mixture is cooled to 40° C., and then the Bletilla striata extract, the Herba Lycopodii extract and the Herba Saussureae extract are added, stirred evenly, and then cooled to room temperature to obtain the spot-lightening and whitening emulsion.

[0074] Example 4

[0075] A freckle-lightening and whitening lotion containing multiple plant extracts comprises the following components in percentage by weight: 3.8% of hexyldecyl alcohol, 2.8% of niacinamide, 1.4% of tranexamic acid, 0.8% of 4-butylresorcinol, 0.8% of hydrolyzed yeast protein, 0.6% of undecylenoylphenylalanine, 0.4% of bletilla striata extract, 1.8% of ophiopogon erythrorhizon extract, 0.4% of snow lotus extract, 4% of moisturizing agent, 3.2% of thickener, 1.8% of emulsifier and 1.4% of filler, and the rest is water.

[0076] The rest is the same as Example 3.

[0077] Comparative Example 1

[0078] In this comparative example, Acanthopanax senticosus saponin B and arbutin were used to replace the spot lightening and whitening lotion intermediate, and the rest was the same as Example 4.

[0079] Comparative Example 2

[0080] In this comparative example, no Bletilla striata extract was added, and the rest was the same as in Example 4.

[0081] Comparative Example 3

[0082] In this comparative example, no Herba Lycopodiellae extract was added, and the rest was the same as in Example 4.

[0083] Comparative Example 4

[0084] In this comparative example, no snow lotus extract was added, and the rest was the same as in Example 4.

[0085] Test Example 1

[0086] Tyrosinase activity inhibition assay

[0087] The samples of each embodiment and comparative example were prepared into a 0.15 mmol / L test sample solution. 200 μL of the test sample solution, 200 μL of PBS buffer (pH=6.80), and 100 μL of 100 U / mL tyrosinase solution were added to a 1.5 mL centrifuge tube in sequence. The mixture was incubated at 37° C. for 10 min in a constant temperature water bath. 200 μL of 1.0 mg / mL L-DOPA solution was added, and the reaction solutions in each tube were quickly transferred to a 96-well plate. The absorbance at a wavelength of 475 nm was detected every 30 s. The change in absorbance per unit time was used as a standard for enzyme activity. Kojic acid was used as a positive control and PBS was used as a blank control. A curve was drawn according to the concentration of the sample and the inhibition rate of tyrosinase activity to calculate the inhibition IC of the sample on tyrosinase activity. 50 value (the concentration of the sample when the tyrosinase activity inhibition rate is 50%, in mg / mL), IC 50 The smaller the value, the stronger the ability of the sample to inhibit tyrosinase activity. Figure 1 .

[0088] The tyrosinase activity inhibition rate was calculated according to the following formula:

[0089] Tyrosinase activity inhibition rate (%) = k 0 -k i / k 0 ×100%,

[0090] Where k 0 The slope of the absorbance change curve of the blank control at 475 nm, k i It is the slope of the absorbance change curve of the sample solution at 475nm.

[0091] In the tyrosinase activity inhibition test, Figure 1 It is clearly shown that the IC values ​​of the samples of Examples 1-4 of the present invention for inhibiting tyrosinase activity are 50The value is significantly lower than that of Comparative Examples 1-4. In the composite emulsion system of the present invention, arbutin plays a key blocking role at the source of melanin generation by virtue of its ability to efficiently inhibit the activity of tyrosinase. Certain groups in the molecular structure of arbutin can specifically bind to the active site of tyrosinase, interfere with the conversion process of tyrosine to melanin, thereby reducing the synthesis of melanin. At the same time, the synergistic effect of various plant extracts further strengthens this inhibitory effect. The saponin compounds in the red hair seven extract interact with tyrosinase through their unique chemical structure, change the conformation of the enzyme or the microenvironment of the active center, make it difficult for tyrosinase to normally catalyze the melanin synthesis reaction, and form a complementary inhibition mechanism with arbutin. This synergistic effect enables the spot-lightening and whitening emulsion of the present invention to more accurately and effectively inhibit tyrosinase activity, thereby reducing the amount of melanin generated at the root, which not only helps to lighten existing spots, but also prevents the appearance of new spots, and lays the foundation for achieving a full range of spot-lightening and whitening effects.

[0092] Test Example 2

[0093] Free radical scavenging assay

[0094] Prepare 0.15mmol / L sample solution in each embodiment and comparative example, take 200μL of sample solution and 600μL of 100μmol / L DPPH solution, mix them evenly, react at 37℃ in the dark for 0.5h, measure absorbance at 517nm, use water as blank control and VC as positive control, calculate DPPH· clearance rate, draw curve according to sample concentration and DPPH· clearance rate, calculate IC of sample to clear DPPH· 50 value (the concentration of the sample when the DPPH clearance rate is 50%, in mg / mL), IC 50 The smaller the value, the stronger its scavenging ability to DPPH· is. See the test results in Figure 2 .

[0095] The DPPH clearance rate was calculated according to the following formula:

[0096] DPPH·scavenging rate (%) = A 0 -A i / A 0 ×100%,

[0097] Among them A 0 is the absorbance value of the control group at 517 nm, A i is the absorbance of the sample solution at 517 nm.

[0098] Depend on Figure 2 It can be seen that the IC values ​​of the samples of Examples 1-4 of the present invention for removing DPPH· 50The value is significantly smaller than that of comparative examples 1-4. In terms of free radical scavenging, the Bletilla striata extract, the Herba Lycopodii extract and the Herba Saussureae extract in the present invention each exert a unique and synergistic antioxidant effect. The polysaccharide components in the Bletilla striata extract, in addition to having a good film-forming and moisturizing effect, the active groups such as hydroxyl groups in its molecular structure can react with free radicals to convert the free radicals into stable compounds, thereby interrupting the chain reaction of free radicals and reducing the damage of free radicals to skin cells. The cyclopentadiene compounds in the Herba Lycopodii extract have strong antioxidant ability, and the conjugated double bond system in its chemical structure can efficiently capture free radicals and make them inactive, thereby eliminating the stimulation of free radicals on tyrosinase and reducing the excessive production of melanin caused by oxidative stress. The flavonoids in the snow lotus extract provide hydrogen atoms to combine with free radicals to form stable free radical intermediates, thereby preventing free radicals from further causing oxidative damage to skin cells. At the same time, they can also inhibit the activity of tyrosinase and reduce melanin synthesis and transport. These plant extracts cooperate with each other to form a multi-level, multi-target antioxidant network, which greatly improves the efficiency and ability of the entire emulsion system to scavenge free radicals. By effectively removing free radicals in the skin, it can delay the aging process of the skin and avoid problems such as decreased skin elasticity, wrinkles and reduced glossiness caused by free radical damage. From an antioxidant perspective, it effectively assists in achieving the spot-lightening and whitening effect, so that the spot-lightening and whitening emulsion of the present invention shows excellent performance in free radical scavenging. Compared with the control group, it has a more significant and comprehensive skin care effect, and can better meet the needs of people with different skin types for skin whitening and health.

[0099] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

[0100] The present invention and its implementation methods are described above, which is not restrictive. The drawings are only one of the implementation methods of the present invention, and the actual application is not limited thereto. In short, if ordinary technicians in the field are inspired by it and design methods and embodiments similar to the technical solution without creativity without departing from the purpose of the invention, they should all fall within the protection scope of the present invention.

Claims

1. A spot-lightening and whitening lotion containing multiple plant extracts, characterized in that: The composition comprises the following percentages by weight: 3.2-4% of hexyldecyl alcohol, 2-3% of niacinamide, 1-1.5% of tranexamic acid, 0.5-1% of 4-butylresorcinol, 0.5-1% of hydrolyzed yeast protein, 0.3-0.8% of undecylenoylphenylalanine, 0.1-0.5% of bletilla striata extract, 1.5-2% of rosmarinus officinalis extract, 0.1-0.5% of snow lotus extract, 2-5% of moisturizing agent, 2.2-3.5% of thickener, 1.5-2% of emulsifier and 1-1.5% of filler, and the rest is water; The spot-lightening and whitening emulsion intermediate is prepared by the following steps: S1, mixing Acanthopanax senticosus saponin B, polyethylene glycol, 1,2-hexanediol and methyl glucose sesquistearate, and heating until each component is dissolved to obtain a first solution; S2, dispersing arbutin and isopropyl myristate and adding them to the first solution, mixing them evenly, cooling them to room temperature to obtain a second solution, and then slowly adding deionized water to the second solution at a stirring speed of 500-800 rpm at room temperature, stirring them evenly, to obtain the spot-lightening and whitening emulsion intermediate; The Bletilla striata extract is prepared by the following steps: drying the Bletilla striata raw material until the moisture is less than 15%, coarsely crushing it to 20 mesh, and then crushing it to 60-120 mesh with an ultrafine grinder to obtain Bletilla striata powder, adding pure water to the Bletilla striata powder in a mass ratio of Bletilla striata: pure water = 1:20-1:25, stirring at room temperature at 30-40rpm, then leaching for 12h, heating to 88-92°C, stirring and extracting for 3h, cooling to room temperature, centrifuging at 10°C and 4000rpm for 30min, collecting the supernatant, transferring it to a single-effect concentrator, concentrating it at 60-65°C to a specific gravity of 1.14-1.16, and obtaining a Bletilla striata extract concentrate, continuously adding the Bletilla striata water extraction supernatant concentrate to anhydrous ethanol, stirring evenly at 60-80rpm until the final ethanol mass concentration is 70-72%, standing for 2h, removing the supernatant and centrifuging at 1400rpm for 15min, and freeze-drying the obtained precipitate to obtain the Bletilla striata extract; The extract of Herba Lycopersicon esculentum is prepared by the following steps: steaming the rhizome of Herba Lycopersicon esculentum at 80-100° C., drying at 40-60° C. for 12-24 hours, mechanically crushing, and then adding 95% ethanol in an amount of 1-3 times the weight of Herba Lycopersicon esculentum for three extractions, combining the extracts, and concentrating under reduced pressure to obtain a Herba Lycopersicon esculentum concentrate, adding 1-3 times the volume of deionized water in an amount of the Herba Lycopersicon esculentum concentrate to form a suspension, and then extracting with ethyl acetate and n-butanol, concentrating the ethyl acetate extraction part and the n-butanol extraction part, purifying by silica gel column chromatography, removing the solvent under reduced pressure, and combining to obtain the Herba Lycopersicon esculentum extract; The snow lotus extract is prepared by the following steps: washing, drying and crushing the snow lotus, adding 3-5 times the weight of 80% methanol to carry out three extractions, centrifuging the extract and then concentrating it to obtain a snow lotus concentrate, mixing it with 3-5 times the volume of petroleum ether of the snow lotus concentrate, taking the water phase, mixing the water phase with n-butanol for extraction, concentrating the n-butanol phase, and separating it with a macroporous resin to obtain a purified solution, concentrating the purified solution under reduced pressure, and drying it to obtain the snow lotus extract.

2. The spot-lightening and whitening emulsion containing multiple plant extracts according to claim 1, characterized in that: In the step S1, the weight ratio of Acanthopanax senticosus saponin B, polyethylene glycol, 1,2-hexanediol and methyl glucose sesquistearate is 15-22:25-35:4-5:10-12.

3. The spot-lightening and whitening emulsion containing multiple plant extracts according to claim 2, characterized in that: In the step S2, the weight ratio of arbutin, isopropyl myristate, the first solution and deionized water is 10-20:5-15:40-60:30-50.

4. The spot-lightening and whitening emulsion containing multiple plant extracts according to claim 3, characterized in that: The silica gel column chromatography purification of the ethyl acetate extraction part is carried out in a system of petroleum ether:ethyl acetate:methanol=7:2:3, and the silica gel column chromatography purification of the n-butanol extraction part is carried out in a system of dichloromethane:ethanol=1:

1.

5. The spot-lightening and whitening emulsion containing multiple plant extracts according to claim 4, characterized in that: The moisturizing agent is one or more of tocopherol acetate, glycerol, propylene glycol, butylene glycol, 1,2-pentanediol, betaine, mannose, trehalose and glycerol polymethacrylate.

6. The spot-lightening and whitening emulsion containing multiple plant extracts according to claim 5, characterized in that: The thickener is one or more of carbomer, xanthan gum, sclerotium gum, laureth-7, beheneth-25, hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, vinylformamide / ammonium acryloyldimethyl taurate copolymer, polysorbate-20, polysorbate-40 and polysorbate-60.

7. The spot-lightening and whitening emulsion containing multiple plant extracts according to claim 6, characterized in that: The emulsifier is one or more of sorbitan isostearate, methyl glucose dioleate, polyglyceryl-3 methyl glucose distearate, ethoxylated methyl glucose distearate, cetearyl alcohol polyoxyethylene ether-20, and isostearyl alcohol polyether-25.

8. The spot-lightening and whitening emulsion containing multiple plant extracts according to claim 7, characterized in that: The filler is one or more of silica, nano-zinc oxide, nano-titanium dioxide, nano-aluminum oxide, nano-boron nitride, polydimethylsiloxane, cyclopolydimethylsiloxane and polymethyl methacrylate.

9. A method for preparing the spot-lightening and whitening emulsion containing multiple plant extracts according to any one of claims 1 to 8, characterized in that: The following steps are involved: (1) mixing the humectant, thickener, filler and water uniformly, heating to 75-85° C., homogenizing and keeping the temperature for 20-30 minutes to obtain a first mixture; (2) adding an emulsifier, hexyldecyl alcohol, niacinamide, tranexamic acid, 4-butylresorcinol, hydrolyzed yeast protein and undecylenoylphenylalanine to the first mixture and stirring to obtain a second mixture; (3) The second mixture is cooled to 40° C., and then the Bletilla striata extract, the Herba Lycopodii extract and the Herba Saussureae extract are added, stirred evenly, and then cooled to room temperature to obtain the spot-lightening and whitening emulsion.

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