Production method of refined ganoderma lucidum spore oil with high activity

Abstract

The invention relates to a production method of refined ganoderma lucidum spore oil with high activity. The method comprises the following steps: adding a multi-element enzyme system into ganoderma lucidum spore powder, mixing and heating to obtain a ganoderma lucidum spore powder mixture; adding an ionic liquid into the ganoderma lucidum spore powder mixture, and ultrasonically extracting ganoderma lucidum spore active components such as ganoderma lucidum triterpenes; taking the extracted ganoderma lucidum spore powder precipitate, extracting crude ganoderma lucidum spore oil by using a supercritical extraction method, adding activated clay, and then removing moisture through steam distillation and a drying agent to obtain dehydrated ganoderma lucidum spore oil; and redissolving the obtained ganoderma lucidum spore active component into the dehydrated ganoderma lucidum spore oil to prepare the refined ganoderma lucidum spore oil with high activity. According to the method, ganoderma lucidum spore active ingredients such as ganoderma lucidum triterpenes are extracted and finally redissolved into the refined ganoderma lucidum spore oil, so that the unpleasant taste of the ganoderma lucidum spore oil is effectively removed, the peroxide value is reduced, and the loss of active substances such as the ganoderma lucidum triterpenes is remarkably reduced.

Description

Technical Field

[0001] The invention relates to the technical field of cosmetics, in particular to a method for producing refined ganoderma lucidum spore oil with high activity. Background Art

[0002] Lingzhi is known as the "king of herbs" in traditional Chinese medicine and has rich medicinal value. Lingzhi spore oil is a high-value natural product extracted from Lingzhi spores. It contains triterpenoids, polyphenols, flavonoids, sterols, fatty acids and other bioactive ingredients, and has multiple biological activities such as anti-oxidation, physical enhancement, beauty and freckle removal, liver protection and detoxification.

[0003] Ganoderma triterpenoids are the main active ingredients in Ganoderma lucidum spore oil. Related studies have shown that they have a variety of important physiological functions, have direct cytotoxic effects on tumor cells, and can play an anti-tumor role through a variety of mechanisms; they have an inhibitory effect on the proliferation of rat hepatic stellate cells and show anti-liver fibrosis activity; they can enhance the activity of immune cells and increase the level of immunoglobulins. At the same time, Ganoderma triterpenoids can also be used in cosmetics. Ganoderma triterpenoids have strong antioxidant capacity, can remove free radicals in the body, increase the activity of antioxidant enzymes, and thus slow down the aging process; in addition, Ganoderma triterpenoids can regulate the balance of skin moisture and oil, improve skin quality, and make the skin smoother and more delicate.

[0004] Although Ganoderma lucidum spore oil and Ganoderma lucidum triterpenes have various beneficial effects, they face many difficulties in practical application. Ganoderma lucidum spore oil has an unpleasant taste that is unique to Ganoderma lucidum, which limits its application prospects in cosmetics. A high peroxide value is also a major problem in the application of Ganoderma lucidum spore oil, which means that the quality of the oil deteriorates, the nutritional value decreases, and the safety issue cannot be guaranteed. However, the high temperature of the frequently used steam distillation refining method can easily destroy the nutritional molecular structure of active substances such as Ganoderma lucidum triterpenes and flavonoids, resulting in the loss of medicinal properties, thereby weakening the auxiliary anti-cancer and liver protection effects of Ganoderma lucidum spore oil. Therefore, how to achieve the refining of Ganoderma lucidum spore oil while maintaining its high activity and achieving a dual improvement in stability and activity has become an industry problem that needs to be overcome urgently. Summary of the invention

[0005] In order to solve the above technical problems, the present invention provides a method for producing highly active refined Ganoderma lucidum spore oil. The present invention extracts active substances from Ganoderma lucidum spores and finally dissolves them in refined Ganoderma lucidum spore oil, which not only effectively removes the unpleasant taste of Ganoderma lucidum spore oil and reduces the peroxide value, but also significantly reduces the loss of active substances such as Ganoderma lucidum triterpenes.

[0006] The object of the present invention is to provide a method for producing highly active refined ganoderma spore oil, comprising the following steps:

[0007] A1, adding a multi-enzyme system to the ganoderma lucidum spore powder, mixing and heating, to obtain a ganoderma lucidum spore powder mixture;

[0008] A2, adding ionic liquid to the Ganoderma lucidum spore powder mixture, ultrasonically extracting the Ganoderma lucidum spore active ingredients and the Ganoderma lucidum spore powder precipitation;

[0009] A3, taking the Ganoderma lucidum spore powder precipitate after extraction in step A2, extracting crude Ganoderma lucidum spore oil by supercritical extraction method, adding activated white clay, and then removing moisture by steam distillation and desiccant to obtain dehydrated Ganoderma lucidum spore oil;

[0010] A4. Redissolving the active ingredients of the ganoderma spores obtained in step A2 into the dehydrated ganoderma spore oil in step A3 to prepare the refined ganoderma spore oil with high activity.

[0011] In some embodiments of the present invention, in step A1, the multi-enzyme system includes two or more of cellulase, chitinase and pectinase. The enzymes in the multi-enzyme system are all commercially available products.

[0012] In some embodiments of the present invention, in step A1, the dosage of the multi-enzyme system is 0.1-10%.

[0013] In some embodiments of the present invention, in step A1, the heating treatment temperature is 35-50° C. and the time is 2-6 hours.

[0014] In some embodiments of the present invention, in step A2, the specific operation of extracting the active ingredients of Ganoderma lucidum spores by ultrasonic extraction using ionic liquid is as follows:

[0015] S1, mixing the Ganoderma lucidum spore powder mixture with the ionic liquid, and performing ultrasonic centrifugation to obtain a supernatant and a Ganoderma lucidum spore powder precipitate;

[0016] S2, passing the obtained supernatant through an AB-8 macroporous resin column, using water and ethanol as eluents, taking the eluate after elution, concentrating and freeze-drying it, and obtaining the active ingredients of the Ganoderma lucidum spores.

[0017] Advantages of the supercritical extraction method used in the present invention: High extraction efficiency: Supercritical fluid has high diffusivity and strong solubility, can quickly penetrate into the interior of Ganoderma lucidum spores, and efficiently extract the oil components therein, with a high extraction rate. Good product quality: Extraction under low temperature and oxygen-free environment can effectively avoid the destruction and deterioration of heat-sensitive substances and easily oxidized components in Ganoderma lucidum spore oil, ensure the quality of the oil, and the obtained product has high purity and few impurities. Environmentally friendly process: Supercritical extraction often uses carbon dioxide as an extractant. Carbon dioxide is non-toxic, harmless, non-flammable, chemically stable, and easily separated from the extract, and will not cause environmental pollution and solvent residues.

[0018] Low operating temperature: It can better preserve the biologically active ingredients in Ganoderma lucidum spore oil, such as ganoderic acid, polysaccharides, etc., so that it can maintain its original physiological activity and efficacy.

[0019] In some embodiments of the present invention, in step S1, the ultrasonic conditions are: temperature controlled at 50-70°C, ultrasonic power at 500-600W, and ultrasonic time at 1-2h.

[0020] In some embodiments of the present invention, in step A2, the ionic liquid includes 1-butyl-3-methylimidazolium tetrafluoroborate ([BMIM]BF 4 ), 1-ethyl-3-methylimidazolium acetate ([EMIM]OAc), 1-hexyl-3-methylimidazolium chloride ([HMIM]Cl), and 1-butyl-3-methylimidazolium hexafluorophosphate ([BMIM]PF 6 )

[0021] In some embodiments of the present invention, in step A3, the conditions of the supercritical extraction method are: the pressure is controlled at 20-40 MPa, the temperature is 40-60° C., and the extraction time is 2-4 h.

[0022] In some embodiments of the present invention, in step A3, the amount of activated clay is 1 wt% to 5 wt%;

[0023] The conditions for steam distillation are as follows: the steam generator is set at a temperature of 90-110°C, the temperature of the Ganoderma lucidum spore oil is maintained at 120-180°C, the vacuum degree is 0.08MPa-0.1MPa, and the distillation time is 1-4h.

[0024] In some embodiments of the present invention, in step A3, the desiccant is anhydrous sodium sulfate;

[0025] The operation steps of removing water by using a desiccant include: adding 5wt% to 10wt% of a desiccant to the crude Ganoderma lucidum spore oil obtained by distilling water, stirring for 1 to 60 minutes, and then centrifuging at 1000 to 10000 rpm to remove anhydrous sodium sulfate.

[0026] The invention adds activated clay to remove the peculiar smell of the ganoderma lucidum spore oil. Meanwhile, the peculiar smell and impurities of the crude ganoderma lucidum spore oil are removed by steam distillation, and then the activated clay is removed by extraction, and finally anhydrous sodium sulfate is added for dehydration.

[0027] In the present invention, in order to effectively extract the active ingredients in Ganoderma lucidum spores, the present invention first uses a multi-enzyme system to treat the cell wall of Ganoderma lucidum spores, thereby facilitating the release of active ingredients. Further, the present invention uses ionic liquids to extract the active ingredients of Ganoderma lucidum spores. Compared with the traditional organic solvent extraction method, the extraction method using ionic liquids can destroy the cell wall / membrane structure of Ganoderma lucidum, promote the release of triterpenoids, and improve the extraction rate. In addition, when the ionic liquid extraction method is used in conjunction with microwave and ultrasonic assisted extraction, the ionic liquid can accelerate the mass transfer process, achieve efficient extraction at a lower temperature, shorten the extraction time, reduce the risk of thermal degradation of Ganoderma lucidum triterpenes, and avoid the damage of high temperature to heat-sensitive components. The method of using multi-enzyme treatment and ionic liquid combined extraction in the present invention effectively ensures the release of active ingredients in Ganoderma lucidum spores.

[0028] The above technical solution of the present invention has the following advantages compared with the prior art:

[0029] 1. The present invention can fully extract the active ingredients in Ganoderma lucidum spores through the technology of multi-enzyme treatment and ionic liquid combined treatment, thereby ensuring the release of the active ingredients in Ganoderma lucidum spores.

[0030] 2. After extracting the active ingredients from the ganoderma spores, the present invention extracts the remaining ganoderma spore oil and further refines it, which can reduce the peroxide value of the ganoderma spore oil and alleviate the unpleasant taste of the ganoderma spore oil, thereby effectively enhancing the stability of the ganoderma spore oil.

[0031] 3. The method of extracting the active ingredients of ganoderma spores and then refining the ganoderma spore oil can obtain stable refined ganoderma spore oil while retaining the active ingredients of the ganoderma spore oil. By combining a variety of advanced technologies, a highly active refined ganoderma spore oil is obtained, which is conducive to the use and promotion of ganoderma spore oil in cosmetics. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] In order to make the content of the present invention more clearly understood, the present invention is further described in detail below according to specific embodiments of the present invention in conjunction with the accompanying drawings, wherein:

[0033] Figure 1 These are the results of measuring the peroxide values ​​of different Ganoderma lucidum spore oils in the examples and comparative examples of the present invention.

[0034] Figure 2 These are the results of determining the total triterpene content of different Ganoderma lucidum spore oils in the examples and comparative examples of the present invention.

[0035] Figure 3 These are the results of determining the total flavonoids content of different Ganoderma lucidum spore oils in the examples and comparative examples of the present invention.

[0036] Figure 4These are the results of determining the total polyphenol content of different Ganoderma lucidum spore oils in the examples and comparative examples of the present invention. DETAILED DESCRIPTION

[0037] The present invention is further described below in conjunction with the accompanying drawings and specific embodiments so that those skilled in the art can better understand the present invention and implement it, but the embodiments are not intended to limit the present invention.

[0038] Determination method related to the present invention

[0039] (1) Determination of triterpenoid content in Ganoderma lucidum

[0040] Accurately weigh 10 mg of oleanolic acid, dissolve it in 50% ethanol aqueous solution, and dilute it to 50 mL. Dilute it to 0.2 mg / mL, 0.16 mg / mL, 0.12 mg / mL, 0.08 mg / mL, and 0.04 mg / mL respectively.

[0041] Take 10 mg of Ganoderma lucidum spore oil, dissolve it in 10 mL of 50% ethanol aqueous solution, mix well, and obtain a sample solution with a concentration of 1 mg / mL.

[0042] Take 0.5 mL of sample and standard solution, place in a stoppered test tube, accurately add 0.5 mL of 8% vanillin ethanol solution (50% ethanol in water), add 4 mL of 80% sulfuric acid solution, shake well, heat at 60°C for 15 min, then cool in a cold water bath for 10 min, take out and measure the absorbance at 541 nm.

[0043] (2) Determination of peroxide value

[0044] The determination method refers to the first method indicator titration method of national standard GB 5009.227-2023. Take 2g of Ganoderma lucidum spore oil sample and add 30mL of acetic acid pentanediol mixed solution (ratio is 3:2) in a conical flask, add 1mL of saturated potassium iodide solution, plug the bottle tightly, shake gently to mix, react in the dark for 3min, add 1mL of starch indicator and mix well, and use 0.01M Na 2 S 2 O 3 Titrate the standard solution until the blue color disappears, record the volume of standard titrant consumed, and calculate the peroxide value. The formula is as follows:

[0045] X1=(V-V0)×c×0.1269 / m×100

[0046] Where: X1-peroxide value, unit is gram / 100 gram (g / 100g);

[0047] V-the volume of standard sodium thiosulfate titration solution consumed by the sample, in milliliters (mL);

[0048] V0-the volume of standard sodium thiosulfate titration solution consumed in the blank test, milliliters (mL);

[0049] c-concentration of standard sodium thiosulfate titration solution, in moles / liter (mol / L);

[0050] 0.1269 - the mass of iodine equivalent to 1.00 mL of standard sodium thiosulfate titration solution [c(Na 2 S 2 O 3 )=1.000mol / L], the unit is gram / millimole (g / mmol);

[0051] m-sample mass, in grams (g)

[0052] 100- conversion factor, used to convert to 100g sample.

[0053] (3) Determination of total polyphenol content

[0054] Table 1 Total polyphenol content test sample information

[0055] Reagents Blank control Standard determination Sample determination Sample control determination water 25μL - - 225μL Gallic acid working solution - 25μL - - sample - - 25μL 25μL 10% Folin 125μL 125μL 125μL - 7.5% sodium carbonate solution 100μL 100μL 100μL -

[0056] 1) Prepare samples in the order and amount shown in the table above.

[0057] 2) After adding 10% Folin phenol reagent, react for 3 to 8 minutes.

[0058] 3) After adding the sodium carbonate solution, leave it at room temperature for 60 minutes and measure the absorbance with a spectrophotometer at a wavelength of 765 nm using a cuvette.

[0059] According to the absorbance, a standard curve is prepared with the standard solution concentration as the X-axis and the absorbance as the Y-axis. The measured (sample absorbance value - sample control absorbance value) is substituted into the standard curve. The measured concentration c1 is obtained.

[0060] Total polyphenols (mg GA / kg) = (c1 / c0)*1000

[0061] c0: original sample concentration (μg / mL)

[0062] (4) Determination of total flavonoid content

[0063] Table 2 Total flavonoids content test sample information

[0064]

[0065]

[0066] Add samples and measure according to the information shown in Table 2 above.

[0067] According to the absorbance, a standard curve is prepared with the standard solution concentration as the X-axis and the absorbance as the Y-axis. The measured (sample absorbance value - sample control absorbance value) is substituted into the standard curve. The measured concentration c1 is obtained.

[0068] Total flavonoids (mg Rutin / kg) = (c1 / c0)*1000

[0069] c0: original sample concentration (mg / mL)

[0070] Example 1: Ionic Liquid Screening

[0071] In order to achieve a high content of active ingredients of Ganoderma lucidum spores in the refined Ganoderma lucidum spore oil, the present invention first considers extracting active ingredients from Ganoderma lucidum spores to avoid the loss of active ingredients of oil in the refining process, and then extracts and refines the remaining oil, thereby finally achieving the dual benefits of stability and activity.

[0072] In order to achieve effective extraction of active substances in Ganoderma lucidum spore powder, different ionic liquids were screened for their extraction effects on active substances in Ganoderma lucidum spore powder. The screened ionic liquids include: 1-butyl-3-methylimidazolium tetrafluoroborate ([BMIM]BF4), 1-ethyl-3-methylimidazolium acetate ([EMIM]OAc), 1-hexyl-3-methylimidazolium chloride ([HMIM]Cl) and 1-butyl-3-methylimidazolium hexafluorophosphate ([BMIM]PF6), and ethanol was used as a dispersant. The specific experimental steps are:

[0073] (1) Ganoderma lucidum spore powder was weighed, and mixed with water in a mass ratio of 1:1, and then 0.5% cellulase, 0.3% chitinase and 0.1% pectinase were added to the total mass of the system, and the mixture was stirred at 40° C. for 4 h.

[0074] (2) After the reaction is completed, the Ganoderma lucidum spore powder is dried by freeze drying. After the drying treatment is completed, four portions of 100 g of the dried Ganoderma lucidum spore powder are taken in parallel and mixed with 400 mL of the above four ionic liquids with a concentration of 0.5 M respectively. Ultrasonic extraction is performed, and the parameters of ultrasonic extraction are set as follows: temperature 60°C, power 600W, time 1.5h. Afterwards, centrifuge (8000rpm, 10min) to collect the supernatant and Ganoderma lucidum spore powder precipitate.

[0075] (3) The obtained supernatant was loaded onto an AB-8 macroporous resin column and eluted with deionized water (1000 mL) and 80% ethanol (300 mL) in sequence; the ethanol eluate was collected, concentrated by rotary evaporation, and then freeze-dried to obtain a solid active substance.

[0076] (4) The total weight of the triterpenoids in the solid active matter was measured respectively, and the total yield of the triterpenoids was calculated. The results are shown in Table 3. The results show that the most triterpenoids can be extracted when [EMIM]OAc is used to extract the active matter from the spores of Ganoderma lucidum.

[0077] (5) The spore powder precipitate obtained in step (2) is transferred to a supercritical device to extract spore oil of Ganoderma lucidum. The supercritical extraction conditions adopted are: pressure of 28 MPa, temperature of 50° C., extraction time of 3 h, and Ganoderma lucidum spore oil is obtained.

[0078] (6) The extracted Ganoderma lucidum spore oil was placed in a round-bottom flask and 2% activated clay was added. The water vapor temperature in the steam distillation device was set to 100°C, the temperature of the Ganoderma lucidum spore oil was set to 140°C, the vacuum degree was 0.1 MPa, and the treatment time was 4 hours. The obtained Ganoderma lucidum spore oil was filtered to remove the activated clay, and then anhydrous sodium sulfate with a weight of 5% of the oil was added and stirred at room temperature for 10 minutes, and centrifuged at 10000 rpm for 10 minutes to obtain dehydrated Ganoderma lucidum spore oil.

[0079] (7) adding the dehydrated Ganoderma lucidum spore oil obtained in step (6) to the solid active substance obtained in step (3), dissolving the solid active substance in the Ganoderma lucidum spore oil by ultrasonic dissolution, and removing the insoluble part by filtering. The filtrate is collected to obtain a highly active refined Ganoderma lucidum spore oil product.

[0080] (8) Slowly add until all the ganoderma triterpenes are dissolved. The content of ganoderma triterpenes is determined by spectrophotometry, and the peroxide value is detected by titration.

[0081] Table 3 Effects of different ionic liquids on the yield of triterpenoids in Ganoderma lucidum

[0082]

[0083] Comparative Example 1:

[0084] Take a commercially available sample of Ganoderma lucidum spore oil and directly perform steam distillation for refining. Add 5% activated clay to the oil, set the water vapor temperature in the steam distillation device to 100°C, the temperature of Ganoderma lucidum spore oil to 180°C, the vacuum degree to 0.1MPa, the content of activated clay in Ganoderma lucidum spore oil to 5%, and the treatment time to 3h. The refined Ganoderma lucidum spore oil is filtered to remove the activated clay, and then anhydrous sodium sulfate of 5% of the weight of the oil is added and stirred at room temperature for 10min, and centrifuged at 10000rpm for 10min to obtain dehydrated Ganoderma lucidum spore oil. The content of Ganoderma lucidum triterpenoids in the refined Ganoderma lucidum spore oil is determined by spectrophotometry, and the peroxide value of the refined Ganoderma lucidum spore oil is determined by titration.

[0085] Comparative Example 2

[0086] Commercially available Ganoderma lucidum spore oil.

[0087] Comparative Example 3

[0088] This comparative example is the same as Example 1, except that in step (2), no ionic liquid is added.

[0089] Test Example 1:

[0090] The peroxide value, triterpene content, total polyphenol content and total flavonoid content of the commercially available Ganoderma lucidum spore oil (Comparative Example 2, Sample 1), the Ganoderma lucidum spore oil obtained in Comparative Example 1 (Sample 2) and the highly active refined Ganoderma lucidum spore oil obtained in Example 1 (Sample 3) were measured respectively. Figure 1 As shown in Figure 1, the peroxide value of Ganoderma lucidum spore oil is significantly reduced through the refining process, wherein the peroxide value of the highly active refined Ganoderma lucidum spore oil (sample 3) obtained by the method of the present invention is reduced by 61.7% compared with the peroxide value of the commercially available Ganoderma lucidum spore oil (sample 1). Figure 2 As shown, the triterpene content of the highly active refined Ganoderma lucidum spore oil obtained by the method of the present invention is comparable to that of the commercially available Ganoderma lucidum spore oil (sample 1), while the triterpene content of the refined Ganoderma lucidum spore oil obtained by the direct steam refining method described in Comparative Example 1 is significantly reduced.

[0091] This indicates that the Ganoderma lucidum spore oil obtained by the method of the present invention can maintain a high content of Ganoderma lucidum triterpenoids while refining the Ganoderma lucidum spore oil. Figure 3 and Figure 4 As shown, the flavonoid content and polyphenol content of the refined Ganoderma lucidum spore oil obtained by the method of the present invention are significantly improved compared with the commercially available Ganoderma lucidum spore oil, with the total flavonoid content increased by 298% and the total polyphenol content increased by 152%. It further illustrates that the refined Ganoderma lucidum spore oil obtained by the method of the present invention improves the biological activity of Ganoderma lucidum spore oil under the condition of ensuring the improvement of the stability of Ganoderma lucidum spore oil.

[0092] Test Example 2:

[0093] Sensory evaluations were performed on commercially available Ganoderma lucidum spore oil (sample 1), Ganoderma lucidum spore oil obtained in Comparative Example 1 (sample 2), and highly active refined Ganoderma lucidum spore oil (sample 3) obtained in Example 1. Evaluation indicators included appearance, odor, and transparency. As shown in Table 4, commercially available Ganoderma lucidum spore oil has a heavy odor, while refined Ganoderma lucidum spore oil samples 2 and 3 have no obvious odor, which is more conducive to the application of Ganoderma lucidum spore oil in cosmetics.

[0094] Table 4 Sensory evaluation of different Ganoderma lucidum spore oils

[0095] Ganoderma Lucidum Spore Oil Appearance odor transparency Sample 1 Light yellow liquid Strong odor, slightly oily Clarity and transparency Sample 2 Light yellow liquid Slight smell, no odor Clarity and transparency Sample 3 Light yellow liquid Slight smell, no odor Clarity and transparency

[0096] Through the above tests, it can be concluded that the Ganoderma lucidum spore oil obtained by the method of the present invention can not only remove the unpleasant taste of the Ganoderma lucidum spore oil, but also enhance the stability of the Ganoderma lucidum spore oil, and at the same time maintain the high activity of the Ganoderma lucidum spore oil.

[0097] Obviously, the above embodiments are merely examples for clear explanation and are not intended to limit the implementation methods. For those skilled in the art, other different forms of changes or modifications can be made based on the above description. It is not necessary and impossible to list all the implementation methods here. The obvious changes or modifications derived from these are still within the protection scope of the invention.

Claims

1. A method for producing highly active refined Ganoderma lucidum spore oil, characterized in that: The following steps are involved: A1, adding a multi-enzyme system to the ganoderma lucidum spore powder, mixing and heating, to obtain a ganoderma lucidum spore powder mixture; A2, adding ionic liquid to the Ganoderma lucidum spore powder mixture, ultrasonically extracting the Ganoderma lucidum spore active ingredients and the Ganoderma lucidum spore powder precipitation; A3, taking the Ganoderma lucidum spore powder precipitate after extraction in step A2, extracting crude Ganoderma lucidum spore oil by supercritical extraction method, adding activated white clay, and then removing moisture by steam distillation and desiccant to obtain dehydrated Ganoderma lucidum spore oil; A4. Redissolving the active ingredients of the ganoderma spores obtained in step A2 into the dehydrated ganoderma spore oil in step A3 to prepare the refined ganoderma spore oil with high activity.

2. The method for producing a highly active refined Ganoderma lucidum spore oil according to claim 1, characterized in that: In step A1, the multi-enzyme system includes two or more of cellulase, chitinase and pectinase.

3. The method for producing a highly active refined Ganoderma lucidum spore oil according to claim 1, characterized in that: In step A1, the dosage of the multi-enzyme system is 0.1-10 wt %.

4. The method for producing a highly active refined Ganoderma lucidum spore oil according to claim 1, characterized in that: In step A1, the heating treatment temperature is 35-50° C. and the time is 2-6 hours.

5. The method for producing a highly active refined Ganoderma lucidum spore oil according to claim 1, characterized in that: In step A2, the specific operation of extracting the active ingredients of Ganoderma lucidum spores by ultrasonic extraction using ionic liquid is as follows: S1, mixing the Ganoderma lucidum spore powder mixture with the ionic liquid, and performing ultrasonic centrifugation to obtain a supernatant and a Ganoderma lucidum spore powder precipitate; S2, passing the obtained supernatant through an AB-8 macroporous resin column, using water and ethanol as eluents, taking the eluate after elution, concentrating and freeze-drying it, and obtaining the active ingredients of the Ganoderma lucidum spores.

6. The method for producing a highly active refined Ganoderma lucidum spore oil according to claim 5, characterized in that: In step S1, the ultrasonic conditions are: temperature controlled at 50-70°C, ultrasonic power at 500-600W, and ultrasonic time at 1-2h.

7. The method for producing a highly active refined Ganoderma lucidum spore oil according to claim 1, characterized in that: In step A2, the ionic liquid includes one or more of 1-butyl-3-methylimidazolium tetrafluoroborate, 1-ethyl-3-methylimidazolium acetate, 1-hexyl-3-methylimidazolium chloride and 1-butyl-3-methylimidazolium hexafluorophosphate.

8. The method for producing a highly active refined Ganoderma lucidum spore oil according to claim 1, characterized in that: In step A3, the conditions of the supercritical extraction method are: pressure controlled at 20-40 MPa, temperature at 40-60° C., and extraction time at 2-4 h.

9. The method for producing a highly active refined Ganoderma lucidum spore oil according to claim 1, characterized in that: In step A3, the amount of activated clay is 1wt% to 5wt%; The conditions for steam distillation are as follows: the steam generator is set at a temperature of 90-110°C, the temperature of the Ganoderma lucidum spore oil is maintained at 120-180°C, the vacuum degree is 0.08MPa-0.1MPa, and the distillation time is 1-4h.

10. The method for producing a highly purified Ganoderma lucidum spore oil according to claim 1, characterized in that: In step A3, the desiccant is anhydrous sodium sulfate; The operation steps of removing water by using a desiccant include: adding 5wt% to 10wt% of a desiccant to the crude Ganoderma lucidum spore oil obtained by distilling water, stirring for 1 to 60 minutes, and then centrifuging at 1000 to 10000 rpm to remove anhydrous sodium sulfate.

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