Multi-effect talcum powder for animals as well as preparation method and application of multi-effect talcum powder

Abstract

The invention discloses multi-effect talcum powder for animals as well as a preparation method and application of the multi-effect talcum powder. The multi-effect talcum powder comprises the following components in percentage by mass: 1-3% of sodium benzoate, 1-3% of glycerol monolaurate, 0.5-1.5% of tea polyphenol, 7-13% of citric acid, 15-25% of corn fiber powder and 54.5-75.5% of diatomite. The talcum powder does not stick to the body, is easy to fall off, has good moisture absorption and drying effects, can improve a humid environment and adsorb odor, is good in acidic stability, has an inactivation effect on escherichia coli, salmonella, staphylococcus aureus and porcine rotavirus, and is safe, non-toxic, free of drug resistance, low in cost and stable in performance when being applied to a livestock and poultry breeding environment.

Description

Technical Field

[0001] The invention relates to the technical field of talcum powder for livestock and poultry breeding, and more specifically, to a multi-effect talcum powder and a preparation method and application thereof. Background Art

[0002] At present, the main method of caring for newborn piglets is to wipe the mucus on their bodies with towels or gauze. Although this method is convenient to operate, it cannot make the skin dry quickly. Repeated washing of used towels is not only labor-intensive, but also easy to cause cross-contamination. With the intensive development of animal husbandry, the number of large-scale pig farms has continued to increase. Under high-density breeding environments, the growth space of pigs has become increasingly narrow, and the epidemics in pig farms have also worsened. Piglet diarrhea is the most common disease in the piglet rearing stage. In pig farms, this type of disease accounts for 70% of the incidence of piglets, causing significant economic losses to the pig farming industry. Especially in the wet and cold winter season, the frequent use of liquid disinfectants will aggravate the high humidity and low temperature of the pig house environment, causing hypothermia, cold stress, and decreased resistance of piglets, which will aggravate piglet diarrhea.

[0003] In addition, sows are weak after giving birth and have low immunity. If the reproductive system and lactation system are not properly cared for, they are easily invaded by pathogenic microorganisms. Therefore, it is crucial to find a talcum powder that can quickly absorb moisture, dry the body surface, is non-sticky, and has a disinfecting effect. Summary of the invention

[0004] The first object of the present invention is to provide a multi-effect talcum powder that can effectively absorb moisture and has the effect of killing bacteria and viruses; the second object of the present invention is to provide a preparation method of the multi-effect talcum powder to reduce the preparation cost; the third object of the present invention is to provide an application of the multi-effect talcum powder.

[0005] In view of this, the technical solution of the present invention is as follows:

[0006] A multi-effect talcum powder comprises, by mass percentage, 1-3% sodium benzoate, 1-3% lauric acid monoglyceride, 0.5-1.5% tea polyphenols, 7-13% citric acid, 15-25% corn fiber powder and 54.5-75.5% diatomaceous earth.

[0007] The present invention provides a method for preparing the above-mentioned multi-effect talcum powder, which comprises mixing the components in proportion and stirring them evenly to obtain the powder.

[0008] The present invention provides an application of the above-mentioned multi-effect talcum powder in preparing a preparation for killing bacteria and / or viruses, which can kill bacteria and / or viruses in a short period of time; the preparation for killing bacteria includes a preparation for killing Escherichia coli, a preparation for killing Salmonella, a preparation for killing Staphylococcus aureus; and a preparation for killing porcine rotavirus.

[0009] Among them, citric acid can effectively lower the pH value of diatomaceous earth, making talcum powder acidic, and can kill most bacteria and viruses under acidic conditions.

[0010] Diatomaceous earth can make talcum powder have better suspension and adsorption properties. Corn fiber powder combined with diatomaceous earth can effectively absorb moisture from the body, and it is not sticky and easy to fall off.

[0011] Sodium benzoate has a broad-spectrum bactericidal effect, and its bactericidal and antibacterial effect is enhanced in acidic media. Monolaurin has antibacterial and antiviral effects, and is not limited by pH. Tea polyphenols not only have antibacterial and antiviral effects, but also have a deodorizing effect.

[0012] Sodium benzoate, monolaurin and tea polyphenols have a synergistic effect in sterilization or killing viruses, and jointly enhance the antibacterial and antiviral effects. Their mechanism of action mainly involves destroying microbial structure, interfering with metabolism and inhibiting enzyme activity. When sterilizing or killing viruses, sodium benzoate, monolaurin and tea polyphenols change the permeability of microbial cell membranes, insert and destroy membrane structures, and react with substances on the membrane; inhibit the activity of multiple enzymes in microbial cells, interfere with energy metabolism, redox and other normal metabolic pathways; bind to viral surface proteins to prevent them from adsorbing host cells, and interfere with the synthesis and assembly of viral nucleic acids and proteins. They work synergistically in multiple ways, destroy the structure and function of microorganisms, interfere with their metabolism, adsorption and replication processes, and thus significantly enhance the killing effect on bacteria and viruses. When the three components exist at the same time, the bactericidal and antiviral effects are significantly better than those of a single component or a combination of some components, reflecting a significant joint synergistic effect.

[0013] Compared with the prior art, the beneficial effects of the present invention include but are not limited to:

[0014] 1. The multi-effect talcum powder provided by the present invention can effectively reduce the pH value of diatomaceous earth through citric acid, making the talcum powder acidic and effectively killing bacteria and viruses, and can also enhance the bactericidal and antibacterial effect of sodium benzoate.

[0015] 2. The multi-effect talcum powder provided by the present invention contains three bactericidal and antiviral agents, namely sodium benzoate, monolaurin and tea polyphenols. The bactericidal effect thereof is not affected by pH but has an enhanced effect. The combined use thereof has a strong bactericidal and viral killing effect.

[0016] 3. The multi-effect talcum powder provided by the present invention has an inactivation effect on Escherichia coli, Salmonella, Staphylococcus aureus, and porcine rotavirus. When applied to livestock and poultry breeding environments, it is safe and non-toxic, has no drug resistance, is low in cost, and has stable performance.

[0017] 4. The multi-effect talcum powder provided by the present invention does not stick to the body and is easy to fall off. At the same time, it can also improve the humid environment and absorb odor, and has a good drying effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 This is a diagram showing the toxicity test of talcum powder solution on MA104 cells. DETAILED DESCRIPTION

[0019] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as limiting the present invention, but should be understood as a more detailed description of certain aspects, features, and embodiments of the present invention.

[0020] It should be understood that the terms described in the present invention are only for describing a particular embodiment and are not intended to limit the present invention. In addition, for the numerical range in the present invention, it should be understood that each intermediate value between the upper and lower limits of the scope is also specifically disclosed. The intermediate value in any stated value or stated range, and each smaller range between any other stated value or intermediate value in the described range is also included in the present invention. The upper and lower limits of these smaller ranges can be independently included or excluded in the scope.

[0021] Unless otherwise indicated, all technical and scientific terms used herein have the same meanings as those generally understood by those skilled in the art. Although the present invention describes only preferred methods and materials, any methods and materials similar or equivalent to those described herein may also be used in the implementation or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials associated with the documents. In the event of a conflict with any incorporated document, the content of this specification shall prevail.

[0022] It will be apparent to those skilled in the art that various modifications and variations may be made to the specific embodiments of the present invention description without departing from the scope or spirit of the present invention. Other embodiments derived from the present invention description will be apparent to those skilled in the art. The present invention description and examples are exemplary only.

[0023] The words “include,” “including,” “have,” “contain,” etc. used in this document are open-ended terms, meaning including but not limited to.

[0024] The purpose of the present invention is to solve the defects of the prior art talcum powder, such as being neutral or alkaline, having poor killing effect on bacteria or viruses that can survive in an acidic environment, and being sticky and having poor killing durability.

[0025] The present invention is further described by the following examples, but is not intended to be limiting of the present invention. Specific experimental conditions and methods not specified in the following examples are conventional methods commonly known to those skilled in the art.

[0026] Example 1

[0027] According to weight ratio, weigh 1% sodium benzoate, 1% monolaurin, 0.5% tea polyphenols, 7% citric acid, 15% corn fiber powder, and 75.5% diatomaceous earth, mix and stir evenly to obtain talcum powder.

[0028] Example 2

[0029] According to weight ratio, weigh 2% sodium benzoate, 2% monolaurin, 1% tea polyphenols, 10% citric acid, 20% corn fiber powder, and 65% diatomaceous earth, mix and stir evenly to obtain talcum powder.

[0030] Example 3

[0031] According to weight ratio, weigh 3% sodium benzoate, 3% monolaurin, 1.5% tea polyphenols, 13% citric acid, 25% corn fiber powder, and 54.5% diatomaceous earth, mix and stir evenly to obtain talcum powder.

[0032] Comparative Example 1

[0033] According to weight ratio, weigh 13% citric acid, 25% corn fiber powder, and 62% diatomaceous earth, mix and stir evenly to obtain talcum powder.

[0034] Comparative Example 2

[0035] According to weight ratio, weigh 3% sodium benzoate, 13% citric acid, 25% corn fiber powder, and 59% diatomaceous earth, mix and stir evenly to obtain talcum powder.

[0036] Comparative Example 3

[0037] According to weight ratio, weigh 3% sodium benzoate, 3% monolaurin, 13% citric acid, 25% corn fiber powder, and 56% diatomaceous earth, mix and stir evenly to obtain talcum powder.

[0038] Experimental Example 1 Determination of pH value of talcum powder

[0039] Weigh 5.00g±0.01g of talcum powder sample, place it in a beaker, add 50mL, 100mL, and 250mL of water without carbon dioxide, respectively, that is, the dilution multiples are 10 times, 20 times, and 50 times, respectively. After stirring for 1 minute, let it stand for 5 minutes, and use a calibrated acidometer to measure the pH value of the suspension. Take the arithmetic mean of the parallel determination results as the determination result.

[0040] As shown in Table 1, the pH of the talcum powders of Examples 1, 2 and 3 is less than 3.7 when diluted within 50 times.

[0041] Table 1: pH value test of talcum powder

[0042]

[0043] Experimental Example 2 pH stability test of talcum powder

[0044] Add talcum powder to water and pig manure respectively, stir for 1 minute, and let it stand for 5 minutes. Measure the pH value every day with a calibrated acidometer for 7 consecutive days.

[0045] As shown in Table 2, after talcum powder was mixed with water and feces respectively, its pH value was relatively stable, and talcum powder could effectively reduce the pH value of feces, making it acidic.

[0046] Table 2: pH stability test of talcum powder

[0047]

[0048] Experimental Example 3 Stability Test of Talcum Powder

[0049] Weigh the talcum powder, put it into a beaker, and leave it open at room temperature. Weigh the talcum powder every day and record the temperature and humidity for 14 consecutive days.

[0050] As shown in Table 3, the weight change of talcum powder is small, which shows that the talcum powder is stable and can be stably stored in the environment.

[0051] Table 3: Talcum powder stability test

[0052]

[0053]

[0054] Experimental Example 4: Water absorption test of talcum powder

[0055] Choose a funnel with a diameter of 9 cm, fold the filter paper, and place it against the wall of the funnel. Place the funnel on a 50 mL measuring cylinder. Weigh 5 g of talcum powder and pour it into the funnel covered with filter paper. Slowly add 50 mL of ultrapure water to the talcum powder, and let the water slowly pass through the talcum powder and filter paper for about 30 minutes. After all the water is added, ensure that no water drops drip from under the filter paper for 3 consecutive minutes. Calculate the water absorption rate based on the amount of water after filtration.

[0056] As shown in Table 4, talcum powder absorbs 5 times its own weight in water.

[0057] Table 4: Hydraulic test of talcum powder series

[0058] Group Weighing weight(g) Amount of water added (mL) Filtered water volume (mL) Water absorption (mL) Example 1 5 50.0 26.8 23.2 Example 2 5 50.0 26.0 24.0 Example 3 5 50.0 25.1 24.9

[0059] Experimental Example 5 Effect of Talcum Powder on Killing Bacteria

[0060] Escherichia coli, Salmonella, and Staphylococcus aureus were inoculated and revived on TSA medium. After culturing at 37°C for 24 hours, single colonies were picked and inoculated into TSB medium, which was placed in a shaker at 180 r / min and 37°C for 24 hours. The bacterial solution was centrifuged at 1500 rpm / min for 5 minutes, the supernatant was removed, and the precipitate was resuspended with sterile saline to make a concentrated bacterial solution.

[0061] The concentrated bacterial solution was diluted with sterile saline to make a 0.5 McFarland unit (1-1.5×108CFU / mL) bacterial suspension, and shaken for use. The bacterial suspension was poured into conical bottles at 100mL / bottle, one of which was a blank control bottle. 10g of talcum powder was added to the test group, stirred evenly (liquid stratification will be slightly formed on the surface), and then allowed to stand for 1h, 2h, 3h, and 4h, and then shaken evenly. 100μL was taken and spread on TSA culture medium, and bacteria were counted after culturing for 24h-48h. A plate control group and a blank control group were set up at the same time. The plate control group was the plate with only culture medium added, without bacterial solution and talcum powder.

[0062] As shown in Table 5, the talcum powder of Example 1-3 has a killing effect on Escherichia coli after 1 hour of use. In the case of lack of some components, the killing effect of Comparative Example 1-3 is reduced.

[0063] As shown in Table 6, the talcum powder of Examples 1-3 has a killing effect on Salmonella after 1 hour of use. In the case of the lack of some components in Comparative Examples 1-3, the killing effect is reduced.

[0064] As shown in Table 7, the talcum powder of Example 1-3 has a killing effect on Staphylococcus aureus after 1 hour of use. In the case of lack of some components, the killing effect of Comparative Example 1-3 is reduced.

[0065] Table 5: Antibacterial effect of talcum powder on Escherichia coli

[0066]

[0067] Table 6: Antibacterial effect of talcum powder on Salmonella

[0068]

[0069] Table 7: Antibacterial effect of talcum powder on Staphylococcus aureus

[0070]

[0071]

[0072] From the sterilization effects recorded in Tables 5-7, it can be seen that the sterilization effects of Comparative Example 1 were significantly reduced in the absence of sodium benzoate, monolaurin and tea polyphenols, and the sterilization effects of Comparative Example 2 and Comparative Example 3 were reduced in the absence of tea polyphenols, indicating that the sterilization effect is optimal only when all components are present. Therefore, it can be judged that there is a synergistic effect between the above-mentioned ingredients.

[0073] Experimental Example 6 The killing effect of talcum powder on porcine rotavirus (PORV)

[0074] This experiment was completed in the laboratory of the College of Veterinary Medicine of Huazhong Agricultural University. The porcine rotavirus epidemic strain (HEBEI-2021 strain) was isolated, identified and preserved by the laboratory of the College of Veterinary Medicine of Huazhong Agricultural University. African green monkey embryonic kidney epithelial cells (MA104) were purchased from the American Type Culture Collection (ATCC). MA104 cells were cultured in DMEM containing 10% fetal bovine serum at 37°C and 5% CO 2 Culture in an incubator.

[0075] 1. Toxicity test of talcum powder solution on cells

[0076] The examples 1-3 and comparative examples 1-3 were prepared into 2% aqueous solutions with sterile deionized water, filtered with a disposable filter membrane (0.22 μm), and 10 μl of each solution was added to MA104 cells cultured in a 96-well plate. Six wells were repeated for each concentration, and the cells were cultured in a 37°C, 5% CO2 incubator for 48 h. After being gently washed three times with DMEM medium, 100 μl of serum-free DMEM medium was added to each well, and then 10 μl of Cell Counting kit-8 (CCK-8) solution was added to each well. The cells were cultured in a 37°C, 5% CO2 incubator for 48 h. 2 After incubation in an incubator for 4 h, the absorbance at 450 nm was measured using an enzyme-labeled instrument.

[0077] The results are as follows Figure 1 As shown, the test shows that the cell activities of Examples 1-3 and Comparative Examples 1-3 are all above 90%, indicating that the talcum powder solution is non-toxic to MA104 cells.

[0078] 2. Test on killing virus by talcum powder

[0079] Weigh 0.5 g of talcum powder of Examples 1-3 and Comparative Examples 1-3 respectively in several 50 ml centrifuge tubes, and act with 1 ml of virus solution (107TCID50) at room temperature for 5 min, 15 min, and 30 min, respectively, then add appropriate amount of DMEM culture medium to prepare suspensions with final concentrations of 1%, 2%, and 5%, mix well, let stand at room temperature for 10 min, take appropriate amount and filter with 0.22 μm filter membrane, and take 250 μl (1%), 125 μl (2%), and 50 μl (5%) respectively to inoculate MA104 cell monolayer (24-well plate). At the same time, virus solution not treated with drugs, suspension and culture medium with only drugs were inoculated as positive control, drug control, and blank control. After incubation at 37°C, 5% CO2 for 0.5-1h, discard the cells and gently wash three times with DMEM culture medium (maintenance medium) containing trypsin at a final concentration of 4μg / ml, then add 300μl of maintenance medium to each well and incubate at 37°C, 5% CO2 for 12h. Observe the cytopathic effect. After 12 hours, the maintenance solution was discarded, and the cells were gently washed three times with PBS. The cells were fixed with 4% paraformaldehyde at 4°C for 0.5 hours, the fixative was discarded, and the cells were gently washed three times with PBS. The membrane was permeabilized with 0.2% Triton X-100 at room temperature for 20 minutes, the permeabilization solution was discarded, the cells were gently washed three times with PBS, and air-dried. 5% skim milk was added, the cells were blocked at 37°C for 2 hours, the blocking solution was discarded, the cells were gently washed three times with PBS, and air-dried. PORV high-immune serum (1:500) was added, the cells were acted at 37°C for 1 hour, the cells were discarded, the cells were gently washed three times with PBS, and air-dried. FITC-labeled goat anti-rabbit IgG secondary antibody (1:1000) was added, the cells were acted at 37°C for 1 hour, the cells were discarded, the cells were gently washed three times with PBS, and air-dried. DAPI (1:1000) was added to stain the nucleus, the cells were acted at 37°C for 20 minutes, the cells were discarded, and the cells were gently washed once with PBS. Finally, 100 μl PBS was added to each well, and the fluorescence was observed under an inverted fluorescence microscope. The test results were statistically analyzed.

[0080] Result judgment criteria: Under the premise that the cell fluorescence positivity rate in the positive control group wells is above 90% and there is no fluorescence signal in the negative control group wells, if there is no fluorescence signal in the experimental group wells, it proves that the virus is completely inactivated by talcum powder; if there is an obvious fluorescence signal in the experimental group wells, it proves that the virus is not completely inactivated by talcum powder.

[0081] The results are shown in Table 8. The talcum powder of Examples 1-3 showed no fluorescence at room temperature for 5 minutes, and had the effect of killing porcine rotavirus.

[0082] Table 8: Fluorescence observation results of talcum powder on PORV at room temperature

[0083]

[0084] Experimental Example 7: Test on the irritation of talcum powder to animal skin

[0085] Several healthy piglets were selected, 20 in each group, and patch tests were performed on their back skin. An appropriate amount of talcum powder was evenly applied to the patch device, applied to the surface of the animal skin, and removed after 24 hours. The skin was observed for irritation reactions such as redness, itching, and erythema. The observation was continued for 7 days, and the results are shown in Table 9. The results show that Comparative Examples 1-3 and Examples 1-3 were non-irritating to the skin.

[0086] Table 9: Skin irritation of talcum powder

[0087] Grouping Total number of heads (heads) Number of stimulation heads (heads) Comparative Example 1 20 0 Comparative Example 2 20 0 Comparative Example 3 20 0 Embodiment 1 talcum powder 20 0 Embodiment 2 talcum powder 20 0 Embodiment 3 talcum powder 20 0

[0088] Experimental Example 8 Talcum Powder Drying Effect

[0089] In the delivery room of a pig farm, the talcum powder prepared by this preparation was compared with traditional towels and other brands of talcum powder for wiping newborn piglets. The results are shown in Table 10.

[0090] Table 10: Talcum powder drying effect

[0091]

[0092] Example 9 Application of talcum powder in pig delivery room

[0093] In a pig farm, liquid disinfectant was used in the delivery room to compare with the talcum powder of the present preparation. The delivery room area was treated with talcum powder as required, the feces were covered with talcum powder, and the lactation area of ​​7-day-old piglets and sows was covered with talcum powder. The comparison results are shown in Table 11. As can be seen from Table 11, the talcum powder of the present preparation can effectively reduce the diarrhea rate of piglets, improve the survival rate of piglets and the rate of healthy piglets at weaning. Comparative Examples 1, 2 and 3 are less effective than Example 1 in the absence of some effective ingredients.

[0094] Table 11: Application of talcum powder in delivery room

[0095] project Liquid disinfectant Comparative Example 1 Comparative Example 2 Comparative Example 3 Embodiment 1 talcum powder Newborn piglets (head) 100 100 100 100 100 Weaned piglets (head) 75 80 83 85 90 Survival rate (%) 75 80 83 85 90 Weaned healthy piglets (head) 53 60 63 67 80 Healthy weaning rate (%) 71 75 76 79 89 Number of weaned piglets with diarrhea (head) 15 13 10 7 4 Diarrhea rate of weaned piglets (%) 20 16 12 8 4

[0096] Experimental Example 10: Method of using talcum powder

[0097] Taking pig farm as an example, the usage of talcum powder is shown in Table 12.

[0098] Table 12: How to use talcum powder

[0099]

[0100]

[0101] Finally, a few points should be explained. Although the present invention has been described in detail above with general descriptions and specific embodiments, on the basis of the present invention, the above embodiments are only used to illustrate the technical solution of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, ordinary technicians in this field should understand that they can still modify the technical solutions recorded in the aforementioned embodiments, or replace some or all of the technical features therein with equivalents. However, these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A multi-effect talcum powder for animals, characterized in that: The composition comprises, by mass percentage, 1-3% sodium benzoate, 1-3% monolaurin, 0.5-1.5% tea polyphenols, 7-13% citric acid, 15-25% corn fiber powder and 54.5-75.5% diatomaceous earth.

2. The method for preparing the multi-effect talcum powder for animals according to claim 1, characterized in that: Mix the components in proportion and stir evenly.

3. Use of the multi-effect talcum powder for animals according to any one of claims 1 to 2 in the preparation of a preparation for killing Escherichia coli.

4. Use of the multi-effect talcum powder for animals according to any one of claims 1 to 2 in the preparation of a preparation for killing Salmonella.

5. Use of the multi-effect talcum powder for animals according to any one of claims 1 to 2 in the preparation of a preparation for killing Staphylococcus aureus.

6. Use of the multi-effect talcum powder for animals according to any one of claims 1 to 2 in the preparation of a preparation for killing rotavirus in pigs.

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