Composite high-altitude plant extract with nourishing effect as well as preparation method and application of composite high-altitude plant extract

Abstract

The invention belongs to the technical field of cosmetics, and particularly relates to a composite high-altitude plant extract with a nourishing effect and a preparation method and application thereof. The composite high-altitude plant extract with the nourishing effect comprises the radix scutellariae alpinae extract, the rhodiola crenulata extract, the leontopodium alpinum extract, the saussurea involucrata extract and other components, contains more active functional components such as total phenols and total flavonoids, and is good in oxidation resistance; the skin glossiness, the skin water content, the percutaneous moisture loss rate and the skin elasticity can be improved, the nourishing effect is achieved, and therefore the technical problem that high-performance and high-altitude plant extracts are lacked in the prior art is solved.

Description

Technical Field

[0001] The present application belongs to the field of cosmetic technology, and in particular relates to a composite high-altitude plant extract with nourishing effects, a preparation method and an application thereof. Background Art

[0002] Nourishing skin care products can improve skin parameters such as skin roughness, glossiness, dermis density, elasticity, etc. The active functional ingredients used in nourishing skin care products can be derived from chemical substances and natural plant extracts.

[0003] Compared with chemical substances, cosmetics formulated with natural plant extracts as active ingredients have many advantages. On the one hand, toxic and harmful substances may be used in the synthesis process of chemical substances, causing potential hazards, while natural plant extracts are derived from natural plants and have higher safety performance. On the other hand, studies have shown that natural components are more easily absorbed by the skin, making the effects of nourishing skin care products more significant and the nourishing effects more prominent. On the other hand, from a psychological point of view, ingredients extracted from natural plant raw materials can make consumers feel more comfortable and at ease.

[0004] Natural plant raw materials from different regions will have different types and contents of active functional ingredients due to the influence of regional climatic environmental factors such as light, temperature, and soil conditions, and their nourishing effects on the skin will also vary. The special ecological environmental conditions of low temperature, strong ultraviolet radiation, and high cold and hypoxia in high altitude areas have caused high altitude plants that have been in this high altitude ecological environment for a long time to have high levels of plant metabolites in their cells that can resist harsh climatic conditions under the influence of long-term natural selection and their own genetic variation. They also contain high levels of various active functional ingredients (such as flavonoids, terpenes, phenols, and amino acids). Therefore, high altitude plant extracts can be used as active functional ingredients used in nourishing skin care products. However, high altitude plants include Rhodiola rosea, alpine Scutellaria baicalensis, alpine Edelweiss, Seabuckthorn, Snow Lotus, Gentiana, and many other plants. Different high altitude plants contain different types and contents of active functional ingredients.

[0005] However, the current research on extracts from high-altitude plants is not in-depth enough, and thus the performance of the prepared high-altitude plant extracts needs to be improved. Summary of the invention

[0006] In view of this, the present application provides a composite high-altitude plant extract with nourishing efficacy, a preparation method and an application thereof, to solve the technical problem of the lack of high-performance high-altitude plant extracts in the prior art.

[0007] The first aspect of the present application provides a composite high-altitude plant extract with nourishing effects, including alpine scutellaria baicalensis extract, rhodiola rosea extract, alpine edelweiss extract and snow lotus extract.

[0008] Preferably, in the composite high-altitude plant extract with nourishing effect, the mass ratio of alpine scutellaria extract, rhodiola rosea extract, alpine edelweiss extract and snow lotus extract is 0.1:0.7:0.1:0.1, 0.4:0.4:0.1:0.1, 0.1:0.4:0.4:0.1, 0.1:0.4:0.4:0.1, 0.1:0.4:0.1:0.4, 0.175:0.475:0.175:0.175, 0.25:0.4:0.1:0.25 or 0.25:0.55:0.1:0.1.

[0009] Preferably, in the composite high-altitude plant extract with nourishing efficacy, the mass ratio of the alpine scutellaria extract, the rhodiola rosea extract, the alpine edelweiss extract and the snow lotus extract is 0.1:0.4:0.1:0.4 or 0.25:0.4:0.1:0.25.

[0010] The second aspect of the present application provides a method for preparing a composite high-altitude plant extract with nourishing efficacy, which can be used to prepare the composite high-altitude plant extract with nourishing efficacy described in the first aspect. The preparation method comprises the steps of:

[0011] The extraction step of the alpine scutellaria baicalensis extract is as follows: adding the alpine scutellaria baicalensis to an ethanol aqueous solution with a concentration of 40-60% according to a mass volume ratio of 1:20-60, ultrasonicating at a temperature of 30-70°C for 20-60 minutes, centrifugally filtering and obtaining the supernatant, and drying to obtain the alpine scutellaria baicalensis extract;

[0012] The step of extracting the Rhodiola rosea extract is as follows: adding the Rhodiola rosea to an ethanol aqueous solution with a concentration of 40-60% at a mass volume ratio of 1:20-60, ultrasonicating at a temperature of 30-70° C. for 20-60 minutes, centrifuging and filtering the supernatant, and drying to obtain the Rhodiola rosea extract;

[0013] The extraction step of the alpine edelweiss extract is as follows: adding the alpine edelweiss to an ethanol aqueous solution with a concentration of 40-60% at a mass volume ratio of 1:20-60, ultrasonicating at a temperature of 30-70°C for 20-60 minutes, centrifugally filtering and obtaining the supernatant, and drying to obtain the alpine edelweiss extract;

[0014] The snow lotus extract is extracted by adding the snow lotus to an ethanol aqueous solution with a concentration of 40-60% according to a mass volume ratio of 1:20-60, ultrasonicating at a temperature of 30-70° C. for 20-60 minutes, centrifugally filtering and taking the supernatant, and drying to obtain the snow lotus extract;

[0015] The compounding step is to mix the alpine scutellaria baicalensis extract, the rhodiola rosea extract, the alpine edelweiss extract and the snow lotus extract to obtain a composite high-altitude plant extract with nourishing effects.

[0016] Preferably, the extraction step of the alpine scutellaria extract is specifically as follows: adding alpine scutellaria to a 60% ethanol aqueous solution at a mass volume ratio of 1:60, ultrasonicating at a temperature of 60°C for 60 minutes, centrifugally filtering the supernatant, and drying to obtain the alpine scutellaria extract.

[0017] Preferably, the extraction step of the Rhodiola rosea extract specifically includes: adding Rhodiola rosea to a 50% ethanol aqueous solution at a mass volume ratio of 1:30, ultrasonicating at 70°C for 20 minutes, centrifugally filtering the supernatant, and drying to obtain the Rhodiola rosea extract.

[0018] Preferably, the extraction step of the alpine edelweiss extract specifically includes: adding the alpine edelweiss to a 60% ethanol aqueous solution at a mass volume ratio of 1:60, ultrasonicating at 70°C for 40 minutes, centrifugally filtering the supernatant, and drying to obtain the alpine edelweiss extract.

[0019] Preferably, the extraction step of the snow lotus extract specifically includes: adding the snow lotus to a 55% ethanol aqueous solution at a mass volume ratio of 1:50, ultrasonicating at a temperature of 60° C. for 60 minutes, centrifugally filtering to obtain the supernatant, and drying to obtain the snow lotus extract.

[0020] The third aspect of the present application provides the use of a composite high-altitude plant extract with nourishing effect described in the first aspect in the preparation of a skin care product.

[0021] Preferably, the application is specifically: application in the preparation of lotion, essence water, essence liquid or facial mask.

[0022] The fourth aspect of the present application provides an essence water with nourishing effect, comprising essence water and a composite high-altitude plant extract with nourishing effect as described in the first aspect.

[0023] Preferably, the mass ratio of the essence water to the composite high-altitude plant extract with nourishing efficacy described in the first aspect is 90:10. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] In order to more clearly illustrate the specific implementation methods of the present application or the technical solutions in the prior art, the drawings required for use in the specific implementation methods or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are some implementation methods of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0025] Figure 1 Schematic diagram of the total phenol content in the high-altitude plant extracts obtained by extracting seabuckthorn, alpine scutellaria, rhodiola rosea, alpine edelweiss, and snow lotus using water extraction, 60% ethanol aqueous solution extraction, and anhydrous ethanol extraction in Example 1 of the present application;

[0026] Figure 2 This is a schematic diagram of the total flavonoid content in the high-altitude plant extracts obtained by extracting seabuckthorn, alpine scutellaria, rhodiola rosea, alpine edelweiss, and snow lotus using water extraction, 60% ethanol aqueous solution extraction, and anhydrous ethanol extraction in Example 1 of the present application;

[0027] Figure 3 This is a schematic diagram of the total phenolic and total flavonoid contents in the high-altitude plant extracts obtained by extracting sea buckthorn, alpine scutellaria, rhodiola rosea, alpine edelweiss, and snow lotus using 60% ethanol aqueous solution in Example 1 of the present application;

[0028] Figure 4 This is a schematic diagram of the DPPH·scavenging rate and ABTS+·scavenging rate of the high-altitude plant extracts obtained by extracting sea buckthorn, alpine scutellaria, rhodiola rosea, alpine edelweiss, and snow lotus using 60% ethanol aqueous solution in Example 1 of the present application;

[0029] Figure 5 The gallic acid and absorbance standard curve used in calculating the total phenol content in the high-altitude plant extract in Example 1 of the present application;

[0030] Figure 6 The rutin and absorbance standard curve used in calculating the total flavonoid content in the high-altitude plant extract in Example 1 of the present application;

[0031] Figure 7 This is the Trolox versus DPPH clearance rate standard curve used when calculating the DPPH clearance rate of high altitude plant extracts in Example 1 of the present application;

[0032] Figure 8 This is the Trolox versus ABTS+· clearance rate standard curve used when calculating the ABTS+· clearance rate of high altitude plant extracts in Example 1 of the present application;

[0033] Fig. 9Schematic diagram of DPPH·scavenging rate of alpine scutellaria extract, Rhodiola rosea extract, alpine edelweiss extract, and snow lotus extract extracted in the examples of the present application, wherein Figure A is the DPPH·scavenging rate of the alpine scutellaria extract, Figure B is the DPPH·scavenging rate of the Rhodiola rosea extract, Figure C is the DPPH·scavenging rate of the alpine edelweiss extract, and Figure D is the DPPH·scavenging rate of the snow lotus extract;

[0034] Fig.10 A schematic diagram of the change in skin glossiness of an essence water containing a composite high-altitude plant extract with nourishing effects provided in an embodiment of the present application and a common essence water;

[0035] Fig.11 A schematic diagram of the change in skin moisture content of an essence water to which a composite high-altitude plant extract with nourishing efficacy provided in an embodiment of the present application is added and a common essence water;

[0036] Fig.12 A schematic diagram of skin elasticity changes of an essence water containing a composite high-altitude plant extract with nourishing efficacy provided in an embodiment of the present application and a common essence water;

[0037] Fig.13 This is a schematic diagram of the changes in transepidermal water loss of the skin after adding an essence water containing a composite high-altitude plant extract with nourishing efficacy provided in an embodiment of the present application and a common essence water. DETAILED DESCRIPTION

[0038] The present application provides a composite high-altitude plant extract with nourishing efficacy, a preparation method and an application thereof, which are used to solve the technical problem of the lack of high-performance high-altitude plant extracts in the prior art.

[0039] The technical solution of the present application will be described clearly and completely below in conjunction with the accompanying drawings. Obviously, the described embodiments are part of the embodiments of the present application, rather than all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present application.

[0040] In view of the fact that the current research on high-altitude plants such as Rhodiola rosea, alpine scutellaria, alpine edelweiss, sea buckthorn, snow lotus, and gentian is not in-depth enough, and there is a lack of high-performance high-altitude plant extracts; the present application provides a composite high-altitude plant extract with nourishing effects, including alpine scutellaria extract, Rhodiola rosea extract, alpine edelweiss extract, and snow lotus extract.

[0041] The present application provides a composite high-altitude plant extract with nourishing effect, which is a composite extract of alpine scutellaria, rhodiola rosea, alpine edelweiss and snow lotus screened from rhodiola rosea, alpine scutellaria, alpine edelweiss, sea buckthorn and snow lotus. The research results show that the high-altitude plant extracts extracted by water extraction, alcohol-water extraction and alcohol extraction have high contents of total phenols and total flavonoids in alpine scutellaria, rhodiola rosea, alpine edelweiss and snow lotus, and the DPPH scavenging rate and ABTS+ scavenging rate, and the antioxidant properties are also better than those of composite high-altitude plant extracts such as sea buckthorn; this shows that the present application obtains a high-altitude plant extract with better performance by compounding alpine scutellaria extract, rhodiola rosea extract, alpine edelweiss extract and snow lotus extract, which has nourishing effect and can be added as an active functional ingredient to nourishing skin care products, thereby overcoming the current defect of lack of high-performance high-altitude plant extracts.

[0042] As a preferred embodiment, in the composite high-altitude plant extract with nourishing effect provided by the present application, the ratio of alpine scutellaria extract, large-flowered rhodiola rosea extract, alpine edelweiss extract and snow lotus extract is also adjusted to obtain a composite high-altitude plant extract with better performance; when the mass ratio of alpine scutellaria extract, large-flowered rhodiola rosea extract, alpine edelweiss extract and snow lotus extract is 0.1:0.7:0.1:0.1, 0.4:0.4:0.1:0.1, 0.1:0.4:0.4:0.1, 0.1:0.4:0.1:0.4, 0.175:0.475:0.175:0.175, 0.25:0.4:0.1:0.25 or 0.25:0.55:0.1 :0.1, the composite high-altitude plant extract of alpine scutellaria extract, rhodiola rosea extract, alpine edelweiss extract and snow lotus extract has a strong antioxidant property, and when the mass ratio of alpine scutellaria extract, rhodiola rosea extract, alpine edelweiss extract and snow lotus extract is 0.1:0.4:0.1:0.4 or 0.25:0.4:0.1:0.25, alpine scutellaria extract, rhodiola rosea extract, alpine edelweiss extract and snow lotus extract can show obvious synergistic effect; that is, the active ingredients in alpine scutellaria extract, rhodiola rosea extract, alpine edelweiss extract and snow lotus extract have a synergistic effect, and the nourishing effect is better than that of rhodiola rosea extract at the same dosage (IC 50 Value is 0.571mg / mL) or alpine edelweiss extract (IC 50 The value is 0.549mg / mL), which is certainly better than the less effective alpine scutellaria extract (IC 50 value of 0.937mg / mL) and snow lotus extract (IC 50The value is 2.308mg / mL).

[0043] As a preferred embodiment, the present application also improves the preparation method of the composite high-altitude plant extract with nourishing effect. By comparing various extraction methods such as water extraction, ethanol-water solution extraction and anhydrous ethanol extraction, the developed ethanol-water solution method can extract more total phenols and total flavonoids from high-altitude plants such as alpine Scutellaria baicalensis extract, Rhodiola rosea extract, alpine Edelweiss extract and Snow Lotus, and improve the antioxidant properties of high-altitude plant extracts.

[0044] As a further preferred embodiment, the present application has made further improvements to the method of extracting high-altitude plants such as alpine scutellaria extract, rhodiola rosea extract, alpine edelweiss extract and snow lotus with ethanol aqueous solution. By improving the concentration of ethanol aqueous solution, the solid-liquid ratio of high-altitude plants and ethanol aqueous solution, and the temperature and time of ultrasonic extraction, more total phenols and total flavonoids are further extracted from high-altitude plants such as alpine scutellaria extract, rhodiola rosea extract, alpine edelweiss extract and snow lotus, and the antioxidant properties of high-altitude plant extracts are further improved.

[0045] Correspondingly, the present application also provides the use of the above-mentioned composite high-altitude plant extract with nourishing effect in the preparation of skin care products such as lotion, essence water, essence liquid or facial mask.

[0046] At the same time, the present application also provides a skin care product, which is an essence water, to which 10% of the above-mentioned composite high-altitude plant extract with nourishing effect is added.

[0047] The following is a detailed description of the composite high-altitude plant extract with nourishing effect provided by the present application in combination with the embodiments and experimental examples.

[0048] Example 1

[0049] Example 1 of the present application provides methods for extracting high-altitude plant extracts from seabuckthorn, alpine scutellaria, rhodiola rosea, alpine edelweiss and snow lotus, respectively.

[0050] The method for extracting a high-altitude plant extract from seabuckthorn comprises: weighing 3 g of seabuckthorn plant powder, adding 150 mL of water, 150 mL of 60% ethanol solution or 150 mL of anhydrous ethanol, ultrasonicating at 40° C. for 30 min, centrifugally filtering and obtaining a supernatant to obtain a seabuckthorn extract;

[0051] The method for extracting a high-altitude plant extract from alpine scutellaria comprises: weighing 3 g of alpine scutellaria plant powder, adding 150 mL of water, 150 mL of 60% ethanol solution or 150 mL of anhydrous ethanol, ultrasonicating at 40° C. for 30 min, centrifugally filtering and collecting the supernatant to obtain an alpine scutellaria extract;

[0052] The method for extracting a high-altitude plant extract from Rhodiola rosea includes: weighing 3 g of Rhodiola rosea plant powder, adding 150 mL of water, 150 mL of 60% ethanol solution or 150 mL of anhydrous ethanol, ultrasonicating at 40° C. for 30 min, centrifugally filtering and collecting the supernatant to obtain a Rhodiola rosea extract;

[0053] The method for extracting a high-altitude plant extract from alpine edelweiss includes: weighing 3 g of alpine edelweiss plant powder, adding 150 mL of water, 150 mL of 60% ethanol solution or 150 mL of anhydrous ethanol, ultrasonicating at 40° C. for 30 min, centrifugally filtering and collecting the supernatant to obtain an alpine edelweiss extract;

[0054] The method for extracting a high-altitude plant extract from snow lotus comprises: weighing 3 g of snow lotus plant powder, adding 150 mL of water, 150 mL of 60% ethanol solution or 150 mL of anhydrous ethanol, ultrasonicating at 40° C. for 30 min, centrifuging and filtering to obtain a supernatant to obtain a snow lotus extract.

[0055] Experimental Example 1

[0056] Experimental Example 1 of the present application tests the total phenolic and total flavonoid contents and DPPH·scavenging rate and ABTS+·scavenging rate of the seabuckthorn extract, alpine scutellaria extract, large-flowered rhodiola rosea extract, alpine edelweiss extract and snow lotus extract provided in Example 1. The test results are as follows: Figure 1-4 shown.

[0057] The process of total phenol test includes: referring to the Folin phenol method for determination, first adding 2.4mL ultrapure water and 0.2mL sample solution or standard solution into a test tube (10mL), then adding 0.2mL Folin phenol reagent, and then mixing the solution with 2mL 7% sodium carbonate aqueous solution, and measuring the absorbance at 750nm after 2h; and using gallic acid as the standard solution, seabuckthorn extract, alpine scutellaria extract, large-flowered rhodiola rosea extract, alpine edelweiss extract and snow lotus extract as sample solutions; wherein, the gallic acid concentration is the horizontal axis (X), and the absorbance (Y) is the vertical axis to draw a standard curve, and the gallic acid mass concentration is 0.05~0.4mg / mL and the absorbance has a good linear relationship, and the linear fitting equation is y = 3.9383x + 0.0642 (R² = 0.988), such as Figure 5 As shown;

[0058] The calculation method of the total phenol test results is as follows: the total phenol content in seabuckthorn extract, alpine scutellaria extract, large-flowered rhodiola rosea extract, alpine edelweiss extract and snow lotus extract is referenced to the standard curve using gallic acid (GA) equivalent (mg GA / g of phenolic compounds equivalent to gallic acid per gram of dry sample). All samples were measured 3 times. The results of the total phenol test are as follows: Figure 1 shown.

[0059] The process of total flavonoids test includes: weighing 20.00 mg of rutin, diluting to 100.00 mL with methanol; preparing a 0.20 mg / mL working stock solution; accurately measuring 0.2, 0.4, 0.6, 0.8, 1.0, and 1.2 mL of the working stock solution, adding water to 1.2 mL, adding 0.2 mL of 5% sodium nitrite solution, shaking, and leaving for 6 minutes, adding 0.2 mL of 10% aluminum chloride / aluminum nitrate solution, shaking, and leaving for 6 minutes, adding 2 mL of 1N sodium hydroxide test solution, and then adding water to 5.0 mL, shaking, and leaving for 15 minutes, using the corresponding reagent as a blank, and measuring the absorbance at a wavelength of 500 nm; wherein, a standard curve is drawn with rutin concentration as the horizontal axis (X) and absorbance (Y) as the vertical axis, and the linear relationship between rutin mass concentration 0.008~0.0048 mg / mL and absorbance is good, and the linear fitting equation is: y = 12.182x -0.0209 (R² = 0.9992), e.g. Figure 6 As shown;

[0060] The calculation method for the total flavonoids test is as follows: the total flavonoids content in seabuckthorn extract, alpine scutellaria extract, large-flowered rhodiola rosea extract, alpine edelweiss extract and snow lotus extract is based on the standard curve using rutin (the number of milligrams of rutin equivalent to flavonoids per gram of dry sample, mg RT / g). All samples were tested 3 times. The results of the total flavonoids test are as follows: Figure 2 shown.

[0061] The process of DPPH scavenging rate test includes: taking 0.5mL seabuckthorn extract, alpine scutellaria extract, large-flowered rhodiola rosea extract, alpine edelweiss extract and snow lotus extract and different concentrations of trolox solution and mixing them with 3mL DPPH working solution, taking another 0.5mL sample solvent (methanol) and mixing it with 3mL DPPH working solution as a blank control, after placing it in the dark at room temperature for 20 minutes, the absorbance value was measured at 517nm, according to the formula DPPH scavenging rate = A 0 -A t / A 0 Calculate the clearance rate, where A 0 A is the blank control; tThe standard curve was drawn with the absorbance after the reaction, the DPPH clearance rate as the ordinate (r), and the standard Trolox solution concentration (μmol / L) as the abscissa (X). The linear fitting equation was y = 0.0034x + 0.0178 (R² = 0.9989), as shown in Figure 7 As shown;

[0062] The calculation method of DPPH scavenging rate is as follows: the free radical scavenging capacity of seabuckthorn extract, alpine scutellaria extract, large-flowered rhodiola rosea extract, alpine edelweiss extract and snow lotus extract is expressed in Trolox equivalent antioxidant capacity (TEAC), that is, the number of micromoles of Trolox required for 1 g of sample dry mass to have the same antioxidant capacity (μmol Trolox / g). The DPPH scavenging rate test results are shown in Figure 4 shown.

[0063] The process of ABTS clearance test includes: taking 0.5mL of seabuckthorn extract, alpine scutellaria extract, large-flowered rhodiola rosea extract, alpine edelweiss extract and snow lotus extract and different concentrations of trolox solution and mixing them with 3mL of ABTS working solution, taking another 0.5mL of sample solvent (methanol) and mixing it with 3mL of ABTS working solution as a blank control, placing it in the dark at room temperature for 20 minutes, and then measuring the absorbance at 517nm. According to the formula ABTS clearance = A 0 -A t / A 0 Calculate the clearance rate, where A 0 A is the blank control; t The absorbance after the reaction was plotted with ABTS·scavenging rate as the ordinate (r) and the concentration of standard Trolox solution (μmol / L) as the abscissa (X). The linear fitting equation was y = 0.0011x + 0.0032 (R² = 0.9992), as shown in Figure 8 As shown;

[0064] The calculation method of ABTS scavenging rate is as follows: the free radical scavenging capacity of seabuckthorn extract, alpine scutellaria extract, large-flowered rhodiola rosea extract, alpine edelweiss extract and snow lotus extract is expressed in Trolox equivalent antioxidant capacity (TEAC), that is, the number of micromoles of Trolox required for 1 g of sample dry mass to have the same antioxidant capacity (μmol Trolox / g). The ABTS scavenging rate test results are shown in Figure 4 shown.

[0065] from Figure 1-8 It can be seen from the experimental results shown that extraction methods such as water extraction, 60% ethanol aqueous solution extraction and anhydrous ethanol extraction will affect the extraction of active functional components such as total phenols and total flavonoids in alpine plants. By comparison, it can be seen that ethanol aqueous solution has the best extraction effect and can extract more active functional components from alpine plants; at the same time, there are also great differences in the active functional components extracted from different alpine plants using ethanol aqueous solution. The content of active functional components in seabuckthorn is relatively low, while the active functional components that can be extracted from alpine scutellaria, rhodiola rosea, alpine edelweiss and snow lotus have higher contents of total phenols and total flavonoids, higher scavenging rates for DPPH· and ABTS·, and higher antioxidant properties.

[0066] Example 2

[0067] Example 2 of the present application provides an experiment on the effects of process parameters such as the concentration of ethanol-water solution, the liquid-to-solid ratio of ethanol-water solution and high-altitude plants such as Scutellaria baicalensis, Rhodiola rosea, Edelweiss alpine and Snow Lotus, and the temperature and time of ultrasonic extraction on the extraction of active functional components of high-altitude plants.

[0068] Among them, the experimental results of the antioxidant properties of Scutellaria baicalensis in high-altitude plants such as the concentration of ethanol-water solution, the liquid-to-solid ratio of ethanol-water solution and high-altitude plants, the temperature and time of ultrasonic extraction and other process parameters are shown in Table 1.

[0069] The experimental results on the antioxidant properties of Rhodiola rosea among high-altitude plants by process parameters such as the concentration of ethanol-water solution, the liquid-to-solid ratio of ethanol-water solution and high-altitude plants, and the temperature and time of ultrasonic extraction are shown in Table 2.

[0070] Table 3 shows the experimental results on the antioxidant properties of alpine edelweiss among high-altitude plants by process parameters such as the concentration of ethanol aqueous solution, the liquid-to-solid ratio of ethanol aqueous solution and high-altitude plants, and the temperature and time of ultrasonic extraction.

[0071] The experimental results on the antioxidant properties of snow lotus among high-altitude plants by process parameters such as the concentration of ethanol aqueous solution, the liquid-to-solid ratio of ethanol aqueous solution and high-altitude plants, and the temperature and time of ultrasonic extraction are shown in Table 4.

[0072] Table 1: Experiments on Scutellaria baicalensis

[0073]

[0074] Table 2: Experiments on Rhodiola rosea

[0075]

[0076] Table 3: Experiments with alpine edelweiss

[0077]

[0078] Table 4: Experiments on snow lotus

[0079]

[0080] As can be seen from Table 1, the concentration of ethanol aqueous solution, the liquid-to-solid ratio of ethanol aqueous solution and high-altitude plants, the temperature and time of ultrasonic extraction and other process parameters affect the extraction yield of active ingredients from alpine Scutellaria baicalensis in the order of C>B>A>D. The optimal extraction conditions at the experimental design level are A5B5C4 D5. Therefore, the optimal extraction process was determined as follows: 60% ethanol concentration, 60:1 liquid-to-solid ratio of ethanol aqueous solution and alpine Scutellaria baicalensis, 60°C extraction temperature and 60 min ultrasonic time.

[0081] As can be seen from Table 2, the concentration of ethanol-water solution, the liquid-to-solid ratio of ethanol-water solution and high-altitude plants, the temperature and time of ultrasonic extraction and other process parameters have an influence on the extraction yield of active ingredients of Rhodiola rosea in the order of C>A>B>D. The optimal extraction conditions at the experimental design level are A3B2C5D1. Therefore, the optimal extraction process is determined as follows: 50% ethanol concentration, 30:1 liquid-to-solid ratio, 70℃ extraction temperature and 20min ultrasonic time.

[0082] As can be seen from Table 3, the concentration of ethanol-water solution, the liquid-to-solid ratio of ethanol-water solution and high-altitude plants, the temperature and time of ultrasonic extraction and other process parameters affect the extraction rate of active ingredients of alpine edelweiss in the order of C>B>A>D. The optimal extraction conditions at the experimental design level are A5B5C5D3. Therefore, the optimal extraction process was determined as follows: 60% ethanol concentration, 60:1 liquid-to-solid ratio, 70°C extraction temperature and 40 min ultrasonic time.

[0083] As can be seen from Table 4, the concentration of ethanol-water solution, the liquid-to-solid ratio of ethanol-water solution and high-altitude plants, the temperature and time of ultrasonic extraction and other process parameters have an influence on the extraction rate of active ingredients of snow lotus in the order of B>A>D>C. The optimal extraction conditions at the experimental design level are A4B4C4D5. Therefore, the optimal extraction process is determined as follows: 55% ethanol concentration, 50:1 liquid-to-solid ratio, 60°C extraction temperature and 60 min ultrasonic time.

[0084] It can be seen from this embodiment that by improving the concentration of ethanol-water solution, the liquid-to-solid ratio of ethanol-water solution and high-altitude plants, and the temperature and time of ultrasonic extraction, more total phenols and total flavonoids can be further extracted from high-altitude plants such as Scutellaria baicalensis, Rhodiola rosea, Edelweiss alpine and Snow Lotus, and the antioxidant properties of high-altitude plant extracts can be further improved.

[0085] Experimental Example 2

[0086] This Experimental Example 2 verifies the optimal conditions for high-altitude plant extraction obtained in Example 2, and uses antioxidant capacity as an evaluation index to record the extraction rate of each active ingredient. The result is expressed as Trolox equivalent antioxidant capacity (TEAC), that is, the number of micromoles of Trolox (mmol Trolox / g) required for 1g of sample dry mass to have the same antioxidant capacity.

[0087] The specific extraction steps of the alpine scutellaria extract are as follows: according to a mass-to-volume ratio of 1:60, 1 g of alpine scutellaria is added to a 60% ethanol aqueous solution, ultrasonicated at 60°C for 60 minutes, and centrifuged to obtain the supernatant to obtain the alpine scutellaria extract; the extraction times are 5 times, and the results are shown in Table 5.

[0088] The extraction steps of the Rhodiola rosea extract specifically include: adding 1 g of Rhodiola rosea to a 50% ethanol aqueous solution at a mass volume ratio of 1:30, ultrasonicating at 70°C for 20 minutes, centrifuging and filtering the supernatant to obtain the Rhodiola rosea extract; the extraction times are 5 times, and the results are shown in Table 6.

[0089] The extraction steps of the alpine edelweiss extract specifically include: adding 1g of alpine edelweiss to a 60% ethanol aqueous solution at a mass volume ratio of 1:60, ultrasonicating at 70°C for 40 minutes, centrifuging and filtering the supernatant to obtain the alpine edelweiss extract; the extraction times are 5 times, and the results are shown in Table 7.

[0090] The extraction steps of the snow lotus extract specifically include: adding 1g of snow lotus to a 55% ethanol aqueous solution at a mass volume ratio of 1:50, ultrasonicating at 60°C for 60 minutes, centrifuging and filtering the supernatant to obtain the snow lotus extract; the extraction times are 5 times, and the results are shown in Table 8.

[0091] Table 5: Antioxidant activity of Scutellaria baicalensis extract

[0092]

[0093] Table 6: Antioxidant capacity of Rhodiola rosea extract

[0094]

[0095] Table 7: Antioxidant capacity of alpine edelweiss extract

[0096]

[0097] Table 8: Antioxidant capacity results of snow lotus extract

[0098]

[0099] It can be seen from Tables 5-8 that the average yield of the alpine Scutellaria baicalensis extract is 64.40 mmol Trolox / g, the average yield of the Rhodiola rosea extract is 291.26 mmol Trolox / g, the average yield of the alpine Edelweiss extract is 45.74 mmol Trolox / g, and the average yield of the Snow Lotus extract is 11.08 mmol Trolox / g; and the RSD is in the range of 2.70~3.67%, indicating that the alpine Scutellaria baicalensis, Rhodiola rosea, alpine Edelweiss extract and Snow Lotus extract of Example 2 of the present application can extract more total phenols and total flavonoids under the corresponding process parameters of ethanol aqueous solution concentration, ethanol aqueous solution and high-altitude plant liquid-to-solid ratio, ultrasonic extraction temperature and time, and the antioxidant properties of the high-altitude plant extracts are improved.

[0100] Example 3

[0101] In Example 3 of the present application, high-altitude plant extracts are extracted under the process parameters of the optimal ethanol-water solution concentration of high-altitude plants, the liquid-to-solid ratio of ethanol-water solution and high-altitude plants, and the temperature and time of ultrasonic extraction provided in Example 2, and then the four high-altitude plant extracts are compounded to provide 19 groups of composite high-altitude plant extracts to study the antioxidant properties of the compounded composite high-altitude plant extracts. Four groups of high-altitude plants are also provided to study the antioxidant properties of single high-altitude plant extracts.

[0102] The process of extracting high altitude plant extracts includes:

[0103] The extraction of the alpine scutellaria extract is as follows: according to a mass volume ratio of 1:60, 1 g of alpine scutellaria is added to a 60% ethanol aqueous solution, ultrasonicated at a temperature of 60°C for 60 minutes, centrifuged to obtain the supernatant, then concentrated by rotary evaporation at 40°C and dried at 40°C to obtain the alpine scutellaria extract;

[0104] The extraction of Rhodiola rosea extract is as follows: according to a mass-to-volume ratio of 1:30, 1 g of Rhodiola rosea is added to a 50% ethanol aqueous solution, ultrasonicated at 70°C for 20 minutes, centrifuged to obtain the supernatant, then rotary evaporated and concentrated the supernatant at 40°C and dried at 40°C to obtain the Rhodiola rosea extract.

[0105] The extraction of alpine edelweiss extract is as follows: according to a mass-to-volume ratio of 1:60, 1 g of alpine edelweiss is added to a 60% ethanol aqueous solution, ultrasonicated at 70°C for 40 minutes, centrifuged to obtain the supernatant, then rotary evaporated and concentrated the supernatant at 40°C and dried at 40°C to obtain the alpine edelweiss extract.

[0106] The extraction of snow lotus extract is as follows: according to a mass-to-volume ratio of 1:50, 1 g of snow lotus is added to a 55% ethanol aqueous solution, ultrasonicated at 60°C for 60 minutes, centrifuged to obtain the supernatant, then rotary evaporated and concentrated the supernatant at 40°C and dried at 40°C to obtain the snow lotus extract.

[0107] First, 4 groups of high-altitude plant extracts were prepared according to formulas A to D shown in Table 9, and then the alpine scutellaria extract, the large-flowered rhodiola rosea extract, and the alpine edelweiss extract were dissolved in ethanol to form a 1 mg / mL high-altitude extract solution, and the snow lotus extract was dissolved in ethanol to form a 2.5 mg / mL high-altitude extract solution; then the four high-altitude plant extracts were compounded according to the mass ratios in formulas 1-19 shown in Table 10 to obtain 19 groups of composite high-altitude plant extracts, and then the 19 groups of composite high-altitude plant extracts were respectively dissolved in ethanol to form 2.5 mg / mL composite high-altitude extract solutions, and the antioxidant capacity was determined.

[0108] Table 9: Four groups of high altitude plant extract formulas

[0109]

[0110] Table 10: 19 groups of compound high altitude plant extract formulas

[0111]

[0112] Among them, the process of testing the antioxidant properties of 4 groups of high-altitude plant extracts includes: preparing 4 groups of high-altitude Scutellaria baicalensis extract solutions (1 mg / mL), Rhodiola rosea extract solutions (1 mg / mL), alpine Edelweiss extract solutions (1 mg / mL), and Snow Lotus extract solutions (2.5 mg / mL) into concentration gradient solutions, and then conducting DPPH·scavenging rate tests. In the test, 0.1 mL of high-altitude plant solutions of different concentrations were taken, 0.4 mL of deionized water was added, and mixed with 3 mL of DPPH working solution respectively. Another 0.5 mL of sample solvent (methanol) was mixed with 3 mL of DPPH working solution as a blank control. After being placed in the dark at room temperature for 20 min, the absorbance was measured at 517 nm. According to the formula DPPH·scavenging rate = A 0 -A t / A 0 Calculate the clearance rate, where A 0 A is the blank control;t The absorbance after the reaction was plotted with mass concentration as the horizontal axis and DPPH scavenging rate as the vertical axis. The antioxidant value of each formula was obtained according to the DPPH scavenging rate curves of the four groups of high-altitude plant extract formulas as IC 50 Value to represent.

[0113] The results of the experimental antioxidant capacity (EAC) tests of the four high-altitude plant extract formulas are as follows: Fig. 9 As shown, from Fig. 9 It can be seen that in the concentration range of 0~1 mg / mL, the alpine scutellaria extract solution, the large-flowered rhodiola rosea extract solution and the alpine edelweiss extract solution have a good linear relationship with the DPPH·scavenging rate. The higher the concentration of the alpine scutellaria extract solution, the large-flowered rhodiola rosea extract solution and the alpine edelweiss extract solution, the higher the DPPH·scavenging rate. In the concentration range of 0~2.5 mg / mL, the snow lotus extract solution has a good linear relationship with the DPPH·scavenging rate. The higher the concentration of the snow lotus extract solution, the higher the DPPH·scavenging rate.

[0114] It can be seen that the IC 50 The value is 0.937mg / mL, and the IC 50 The value is 0.571mg / mL, and the IC 50 The value is 0.549mg / mL, and the IC 50 The value is 2.308 mg / mL; this shows that alpine edelweiss has the best scavenging effect on DPPH·, and can achieve a higher DPPH· clearance rate at a lower concentration, while snow lotus has a poor scavenging effect on DPPH·, and requires a higher concentration to achieve a higher DPPH· clearance rate.

[0115] The testing process of the antioxidant properties of 19 groups of composite high-altitude plant extracts included experimental antioxidant capacity (EAC) testing and theoretical antioxidant capacity (TAC) calculation.

[0116] The theoretical antioxidant capacity (TAC) of the composite high-altitude plant extract is related to the sum of the experimental antioxidant capacity of the single high-altitude plant extracts in the four groups of high-altitude plant extracts. The calculation formula is: TAC = W a ×T a +W b ×T b + W c ×T c+ W d ×T d , where T a -T d - The antioxidant capacity of the extracts of Scutellaria baicalensis, Rhodiola rosea, Leontopodium edulis, and Snow Lotus in single use of the same fixed dose, W a -W d They represent the weight of the antioxidant capacity of the single component of the dose of each extract in the composite sample. The theoretical antioxidant capacity (TAC) of the composite high-altitude plant extracts is shown in Table 11. Taking the theoretical antioxidant capacity (TAC) of the composite high-altitude plant extracts of Group 1 as an example, the calculation process is (0.937±0.019)×0.7+(0.571±0.004)×0.1+(0.549±0.024)×0.1+(2.308±0.059)×0.1;

[0117] The experimental antioxidant performance (EAC) test process is as follows: referring to the antioxidant performance test process of 4 groups of high-altitude plant extracts, 19 groups of 2.5 mg / mL composite high-altitude plant extract solutions were diluted into solutions with a concentration gradient of 0-2.5 mg / mL, 0.1 mL was added with 0.4 mL of deionized water, and mixed with 3 mL of DPPH working solution, and 0.5 mL of sample solvent (methanol) was mixed with 3 mL of DPPH working solution as a blank control. After being placed in the dark at room temperature for 20 min, the absorbance was measured at 517 nm. According to the formula DPPH·scavenging rate = A 0 -A t / A 0 Calculate the clearance rate, where A 0 A is the blank control; t The absorbance after the reaction was plotted with mass concentration as the horizontal axis and DPPH scavenging rate as the vertical axis. The antioxidant value of each formula was obtained according to the DPPH scavenging rate curves of 19 groups of compound high-altitude plant extract formulas as IC 50 The experimental antioxidant activity (EAC) test results of the composite high altitude plant extracts are shown in Table 11.

[0118] At the same time, the synergistic effect (SE) of the antioxidant performance of the composite high-altitude plant extracts was judged by comparing the experimental antioxidant performance (EAC) and the theoretical antioxidant capacity (TAC) of the composite high-altitude plant extracts. The ratio of the experimental antioxidant capacity (EAC) to the theoretical antioxidant capacity (TAC) was used for judgment, and the synergistic effect (SE) calculation formula was TAC / EAC; when the synergistic effect (SE) was greater than 1, it meant that the test value of the experimental antioxidant capacity (EAC) was less than the calculated value of the theoretical antioxidant capacity (TAC), the IC50 value was lower, the experimental antioxidant capacity (EAC) was greater than the theoretical antioxidant capacity (TAC), and the extracts of Scutellaria baicalensis, Rhodiola rosea, Leontopodium alba, and Snow Lotus in the composite high-altitude plant extracts could functionally support each other, which was a synergistic effect, and when it was equal to 1, it meant that the experimental antioxidant capacity (EAC) was equal to the theoretical antioxidant capacity (TAC), which was an additive effect, and when it was less than 1, it meant that the experimental antioxidant capacity (EAC) was less than the theoretical antioxidant capacity (TAC), which was an antagonistic effect.

[0119] Among them, the test results of the synergistic effect (SE) of the antioxidant properties of 4 groups of high-altitude plant extracts and 19 groups of composite high-altitude plant extracts are shown in Table 11.

[0120] Table 11: Test results of synergistic effect (SE) of antioxidant performance

[0121]

[0122] As can be seen from Table 11, the experimental antioxidant capacity (EAC) of the 19 groups of composite high-altitude plant extracts is greater than the theoretical antioxidant capacity (TAC), indicating that the alpine scutellaria extract, Rhodiola rosea extract, alpine edelweiss extract and snow lotus in the composite high-altitude plant extract provided by the present application have mutual synergy; at the same time, when the mass ratios of the alpine scutellaria extract, Rhodiola rosea extract, alpine edelweiss extract and snow lotus extract in the composite high-altitude plant extract are different, the effects shown are different. When the alpine scutellaria extract, Rhodiola rosea extract, alpine edelweiss extract and snow lotus extract are in the ratio of 0.1:0.7:0.1:0.1, 0.4:0.4:0.1:0. 1. When the mass ratios are 0.1:0.4:0.4:0.1, 0.1:0.4:0.1:0.4, 0.175:0.475:0.175:0.175, 0.25:0.4:0.1:0.25 or 0.25:0.55:0.1:0.1, they can exhibit strong experimental antioxidant properties (EAC); when the alpine scutellaria baicalensis extract, the large-flowered rhodiola rosea extract, the alpine edelweiss extract and the snow lotus extract are in the mass ratios of 0.1:0.4:0.1:0.4 or 0.25:0.4:0.1:0.25, they can exhibit obvious synergistic effects, show the strongest scavenging rate for DPPH free radicals, and have the best antioxidant effect.

[0123] Example 4

[0124] The present application conducts a nourishing efficacy experiment on the composite high-altitude plant extract provided in Example 3, which is prepared with alpine scutellaria baicalensis extract, rhodiola rosea extract, alpine edelweiss extract and snow lotus extract in a ratio of 0.1:0.7:0.1:0.1.

[0125] Nourishing efficacy experiments include:

[0126] The steps for preparing the experimental samples were to add 10% of the complex high-altitude plant extract to the essence water basic formula as the test product A, and the essence water basic formula as the control product B.

[0127] The process of the nourishing efficacy experiment includes: screening healthy women or men aged 18-60, with dull, dry and rough facial skin; no allergic diseases, no history of allergies to cosmetics or other topical preparations; no history of photosensitivity diseases, and no recent use of drugs that affect photosensitivity; the skin of the test site should be free of inflammation; 5-10 volunteers use the product on their faces for 28 days, and measure the skin gloss, moisture content of the skin stratum corneum, skin elasticity, and transepidermal water loss rate before the test and 7 days, 14 days, and 28 days after using the product. The location of each test area for each volunteer is as consistent as possible, and the left and right face skin of the volunteer is the test group A and the control group B (left A and right B). All operations are completed by the same person to reduce accidental errors. On the day of the test, the volunteers did not use the product. After cleaning the test site, it was necessary to balance at room temperature of 21±1℃ and relative humidity of 55%±5% for at least 30 minutes before testing. SPSS22.0 and Excel software were used to organize and analyze the data. After using the product, the skin gloss, skin water content, skin elasticity, skin transepidermal water loss change (△ difference) and change rate (W) are calculated by the formula W=(T a -T b ) / T a ×100% calculation, △ difference = T a -T b , in the formula, T a is the data before use, T b For post-use data.

[0128] The results of the nourishing efficacy experiment are shown in Tables 12 and Figure 10-13 As shown;

[0129] Table 12: Experimental results of nourishing efficacy experiment

[0130]

[0131] From Table 12 and Figure 10-13It can be seen that compared with the control area experiment, the skin glossiness of the test area was improved by 9.01%, 3.5% and 1.71% respectively, the skin water content of the test area was improved by 15.09%, 10.87% and 4.15% respectively, and the transepidermal water loss rate in the test area was reduced by 3.30%, 7.89% and 3.62% respectively compared with the control area. At the same time, the skin elasticity of the test area was improved by 11.39%, 13.19% and 13.66% respectively compared with the control area. This shows that adding the composite high-altitude plant extract provided by this application to the essence water can improve the skin gloss, skin water content, transepidermal water loss rate and skin elasticity. The nourishing effect is defined in the group standard (T / CAB 0152-2022) as: it helps to improve two or more of the four skin parameters such as skin roughness, gloss, dermis density and elasticity, which can be used as the basis for the product to have a nourishing effect; therefore, the composite high-altitude plant extract provided in this application is a nourishing composite high-altitude plant extract with a nourishing effect.

[0132] The above embodiments are only used to illustrate the technical solutions of the present application, rather than to limit them. Although the present application has been described in detail with reference to the aforementioned embodiments, a person skilled in the art should understand that the technical solutions described in the aforementioned embodiments may still be modified, or some or all of the technical features thereof may be replaced by equivalents. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the scope of the technical solutions of the embodiments of the present application.

Claims

1. A composite high-altitude plant extract with nourishing effect, characterized in that: Including alpine scutellaria extract, rhodiola rosea extract, alpine edelweiss extract and snow lotus extract.

2. The composite high-altitude plant extract with nourishing effect according to claim 1, characterized in that: In the composite high-altitude plant extract with nourishing effect, the mass ratio of alpine scutellaria extract, large-flowered rhodiola rosea extract, alpine edelweiss extract and snow lotus extract is 0.1:0.7:0.1:0.1, 0.4:0.4:0.1:0.1, 0.1:0.4:0.4:0.1, 0.1:0.4:0.1:0.4, 0.175:0.475:0.175:0.175, 0.25:0.4:0.1:0.25 or 0.25:0.55:0.1:0.

1.

3. The composite high-altitude plant extract with nourishing effect according to claim 1, characterized in that: In the composite high-altitude plant extract with nourishing effect, the mass ratio of alpine scutellaria extract, rhodiola rosea extract, alpine edelweiss extract and snow lotus extract is 0.1:0.4:0.1:0.4 or 0.25:0.4:0.1:0.

25.

4. A method for preparing a composite high-altitude plant extract with nourishing effect as claimed in any one of claims 1 to 3, characterized in that: The following steps are involved: The extraction step of the alpine scutellaria baicalensis extract is as follows: adding the alpine scutellaria baicalensis to an ethanol aqueous solution with a concentration of 40-60% according to a mass volume ratio of 1:20-60, ultrasonicating at a temperature of 30-70°C for 20-60 minutes, centrifugally filtering and obtaining the supernatant, and drying to obtain the alpine scutellaria baicalensis extract; The step of extracting the Rhodiola rosea extract is as follows: adding the Rhodiola rosea to an ethanol aqueous solution with a concentration of 40-60% at a mass volume ratio of 1:20-60, ultrasonicating at a temperature of 30-70° C. for 20-60 minutes, centrifuging and filtering the supernatant, and drying to obtain the Rhodiola rosea extract; The extraction step of the alpine edelweiss extract is as follows: adding the alpine edelweiss to an ethanol aqueous solution with a concentration of 40-60% at a mass volume ratio of 1:20-60, ultrasonicating at a temperature of 30-70°C for 20-60 minutes, centrifugally filtering and obtaining the supernatant, and drying to obtain the alpine edelweiss extract; The snow lotus extract is extracted by adding the snow lotus to an ethanol aqueous solution with a concentration of 40-60% according to a mass volume ratio of 1:20-60, ultrasonicating at a temperature of 30-70° C. for 20-60 minutes, centrifugally filtering and taking the supernatant, and drying to obtain the snow lotus extract; The compounding step is to mix the alpine scutellaria baicalensis extract, the rhodiola rosea extract, the alpine edelweiss extract and the snow lotus extract to obtain a composite high-altitude plant extract with nourishing effects.

5. The method for preparing a composite high-altitude plant extract with nourishing effect according to claim 4, characterized in that: The extraction steps of the alpine scutellaria extract are specifically as follows: adding alpine scutellaria to a 60% ethanol aqueous solution at a mass volume ratio of 1:60, ultrasonicating at a temperature of 60° C. for 60 minutes, centrifugally filtering to obtain the supernatant, and drying to obtain the alpine scutellaria extract.

6. The method for preparing a composite high-altitude plant extract with nourishing effect according to claim 4, characterized in that: The extraction step of the Rhodiola rosea extract specifically includes: adding Rhodiola rosea to a 50% ethanol aqueous solution at a mass volume ratio of 1:30, ultrasonicating at a temperature of 70°C for 20 minutes, centrifugally filtering to obtain the supernatant, and drying to obtain the Rhodiola rosea extract.

7. The method for preparing a composite high-altitude plant extract with nourishing effect according to claim 4, characterized in that: The extraction step of the alpine edelweiss extract specifically includes: adding the alpine edelweiss to a 60% ethanol aqueous solution at a mass volume ratio of 1:60, ultrasonicating at a temperature of 70°C for 40 minutes, centrifugally filtering to obtain the supernatant, and drying to obtain the alpine edelweiss extract.

8. The method for preparing a composite high-altitude plant extract with nourishing effect according to claim 4, characterized in that: The extraction step of the snow lotus extract specifically includes: adding the snow lotus to an ethanol aqueous solution with a concentration of 55% according to a mass-to-volume ratio of 1:50, ultrasonicating at a temperature of 60° C. for 60 minutes, centrifugally filtering to obtain the supernatant, and drying to obtain the snow lotus extract.

9. Use of the composite high-altitude plant extract with nourishing effect as claimed in any one of claims 1 to 3 in the preparation of skin care products.

10. An essence water with nourishing effect, characterized in that: The invention comprises essence water and a composite high-altitude plant extract with nourishing effect as claimed in any one of claims 1 to 3.

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