Anti-aging repair composition containing rose extracellular vesicles and application

Abstract

The invention discloses an anti-aging repairing composition containing rose extracellular vesicles and application, and belongs to the technical field of cosmetics, the anti-aging repairing composition containing the rose extracellular vesicles comprises the rose extracellular vesicles, crithmum maritimum extract and hydrolyzed lupin protein; the mass ratio of the rose extracellular vesicles to the crithmum maritimum extract to the hydrolyzed lupin protein is 1: (0.6-2.5): (0.5-2.4). The composition has the functions of resisting oxidation, resisting inflammation and promoting collagen synthesis to repair the skin barrier, comprehensively repairs the skin barrier and relieves skin sensitivity, redness and swelling, meanwhile, the antioxidant and anti-inflammatory effects generate a superimposed effect, damage of oxidative stress and inflammation to the skin can be more effectively reduced, and the skin can be effectively protected. The regeneration and repair of skin cells are promoted from different angles, the self-repair capability of the skin is enhanced, and the skin aging is delayed.

Description

Technical Field

[0001] The invention relates to the technical field of cosmetics, and in particular to an anti-aging and repairing composition containing rose extracellular vesicles and an application thereof. Background Art

[0002] Extracellular vesicles (EVs) are a type of natural vesicles secreted by cells, with sizes ranging from nanometers to micrometers. Extracellular vesicles interact with target cells through the biological molecules such as proteins, lipids, and RNA contained in them, transmit information, and regulate cell metabolism, repair, and immune response. Plant extracellular vesicles have good biocompatibility, are non-toxic, and have rich ingredients.

[0003] As we age, our skin will gradually age. Skin aging is a complex physiological process that is affected by many factors, such as ultraviolet radiation, environmental pollution, stress, and bad living habits. Aging skin is characterized by increased wrinkles, decreased elasticity, dull complexion, dryness, and roughness. At the same time, due to various external factors and internal physiological changes, the skin is also prone to sensitivity, inflammation, and barrier damage, which require repair.

[0004] In view of this, this application is filed. Summary of the invention

[0005] The purpose of the present invention is to overcome the shortcomings of the prior art and provide an anti-aging and repairing composition containing rose extracellular vesicles and its application. The anti-aging and repairing composition containing rose extracellular vesicles of the present invention can significantly improve skin hydration and skin elasticity, reduce transepidermal water loss and wrinkle values, and has excellent anti-aging and repairing effects.

[0006] To achieve the above-mentioned purpose, the first aspect of the present application provides an anti-aging repair composition containing rose extracellular vesicles, including rose extracellular vesicles, sea fennel extract and hydrolyzed lupin protein; the mass ratio of the rose extracellular vesicles, sea fennel extract and hydrolyzed lupin protein is 1: (0.6~2.5): (0.5~2.4).

[0007] Among them, the active ingredients in rose extracellular vesicles stimulate the proliferation and differentiation of skin cells, promote the synthesis of collagen and elastin, promote the regeneration and repair of skin cells, thereby enhancing the elasticity and firmness of the skin. Rose extracellular vesicles have excellent antioxidant effects, can neutralize free radicals, reduce oxidative stress damage to the skin, and inhibit the release of inflammatory factors, relieve skin inflammation, and at the same time, rose extracellular vesicles can improve the stability and utilization of other active ingredients.

[0008] Among them, hydrolyzed lupin protein is rich in amino acids and small molecule peptides, which can stimulate the synthesis of laminin 5 in the skin, promote the production of type IV collagen, enhance the elasticity and firmness of the skin, effectively repair the skin barrier, reduce water loss, improve the skin's moisturizing ability, relieve skin inflammation, and promote the repair of damaged skin.

[0009] Sea fennel extract has antioxidant and anti-free radical properties: Sea fennel extract is rich in polyphenols and flavonoids, has strong antioxidant capacity, can scavenge free radicals, and protect skin cells from oxidative damage. At the same time, sea fennel extract can stimulate the regeneration of skin cells, accelerate wound healing, enhance skin barrier function, and reduce water loss. Sea fennel extract also has soothing and anti-inflammatory effects, can relieve skin sensitivity and redness, and is suitable for the repair of sensitive skin and damaged skin.

[0010] The present invention creatively combines rose extracellular vesicles, sea fennel extract and hydrolyzed lupine protein according to the above-mentioned specific ratio. Under the synergistic effect of the three, the composition has the effects of anti-oxidation, anti-inflammatory, promoting collagen synthesis and repairing skin barrier function, comprehensively repairing the skin barrier, relieving skin sensitivity and redness and swelling, and at the same time, the antioxidant and anti-inflammatory effects produce a superimposed effect, which can more effectively reduce the damage to the skin caused by oxidative stress and inflammation, promote the regeneration and repair of skin cells from different angles, enhance the skin's self-repair ability, and delay skin aging.

[0011] Among them, the rose extracellular vesicles mentioned in the present invention can be prepared according to the method disclosed in the prior applications CN202411924386.5 and CN202311731566.7 of the applicant of this application.

[0012] The method for preparing rose extracellular vesicles comprises the following steps:

[0013] Mix rose flowers and PBS buffer to extract juice, filter and obtain filtrate;

[0014] adding cellulase, xylanase and β-glucanase to the filtrate, performing enzymolysis, filtering and obtaining an enzymolysis solution;

[0015] The enzymatic hydrolysate was centrifuged, filtered, and the precipitate was resuspended to obtain the extracellular vesicles.

[0016] The filtration may be performed using conventional filter cloth and / or filter membrane in the art.

[0017] Wherein, the centrifugation is carried out in an ultracentrifuge.

[0018] The centrifugal temperature is 0-6°C.

[0019] Among them, PBS buffer is preferably used for resuspending the precipitate.

[0020] In one embodiment, the solid-liquid ratio of the rose to PBS buffer is 1 g: (3-6) mL.

[0021] In one embodiment, the mass ratio of the cellulase, xylanase, β-glucanase, and filtrate is (0.01-0.03): (0.01-0.02): (0.01-0.02): 1; and / or

[0022] In the preparation method of rose extracellular vesicles, the enzymatic hydrolysis temperature is 45-55° C. and the enzymatic hydrolysis time is 2-6 hours.

[0023] As an implementation scheme of the present application, the mass ratio of the rose extracellular vesicles, sea fennel extract and hydrolyzed lupin protein is 1:(1.2-1.5):(1.5-1.8). Especially when the dosage of the three is within this range, the three have a more ideal synergistic effect and can more significantly improve the anti-aging and repair effect.

[0024] As an embodiment of the present application, the preparation method of the sea fennel extract is:

[0025] The whole sea fennel herb is crushed to obtain sea fennel powder, and the sea fennel powder, pectinase, cellulase, and hemicellulase are added into water for enzymolysis to obtain an enzymolysate;

[0026] The enzymatic hydrolysate is added into a low eutectic solvent, subjected to ultrasonic extraction and centrifugation to obtain the sea fennel extract.

[0027] As an implementation scheme of the present application, the mass ratio of the sea fennel powder, pectinase, cellulase, hemicellulase and water is 1: (0.008-0.02): (0.005-0.015): (0.002-0.01): (5-20).

[0028] As an implementation scheme of the present application, the enzymolysis temperature is 42 to 52° C., and the enzymolysis time is 1 to 8 hours.

[0029] As an embodiment of the present application, the low eutectic solvent includes betaine, allantoin, glycerol and water in a mass ratio of 1: (0.5-1.2): (2-4): (0.5-2).

[0030] As an implementation scheme of the present application, the power of the ultrasonic extraction is 200-500W, the time is 0.5-2h, and the temperature is 45-65°C.

[0031] As an implementation scheme of the present application, the mass ratio of the enzymatic hydrolysate to the low eutectic solvent is 1:(2-6).

[0032] The second aspect of the present application provides an anti-aging and repairing composition containing rose extracellular vesicles for use in the preparation of cosmetics.

[0033] As an embodiment of the present application, the dosage form of the cosmetic is an aqueous solution, emulsion, cream, gel or mask.

[0034] The third aspect of the present application provides an essence, comprising the following components in weight percentage: 0.1-10% of an anti-aging repair composition containing rose extracellular vesicles, 0.1-0.5% of ammonium acryloyldimethyltaurate / VP copolymer, 0.2-0.8% of 1,2-hexanediol, 0.2-0.8% of p-hydroxyacetophenone, 0.2-1% of tocopherol acetate, 1-4% of squalane, 2-5% of butanediol, and 77.9-96.2% of water;

[0035] The anti-aging and repairing composition containing rose extracellular vesicles is the anti-aging and repairing composition containing rose extracellular vesicles described above.

[0036] The preparation method of the essence is as follows: water, butylene glycol, squalane, tocopherol acetate, and ammonium acryloyldimethyltaurate / VP copolymer are mixed uniformly at 60-70° C., cooled to 35-45° C., the remaining materials are added, stirred uniformly, discharged, cooled to room temperature, and the essence is obtained.

[0037] The beneficial effect of the present invention is that: the present invention combines rose extracellular vesicles, sea fennel extract and hydrolyzed lupin protein according to the above-mentioned specific ratio. Under the synergistic effect of the three, the composition has the effects of anti-oxidation, anti-inflammatory, promoting collagen synthesis and repairing skin barrier function, comprehensively repairing the skin barrier, relieving skin sensitivity and redness and swelling, and at the same time, the antioxidant and anti-inflammatory effects produce a superimposed effect, which can more effectively reduce the damage of oxidative stress and inflammation to the skin, promote the regeneration and repair of skin cells from different angles, enhance the self-repair ability of the skin, and delay skin aging. DETAILED DESCRIPTION

[0038] In order to make the purpose, technical solutions and advantages of the embodiments of the present application clearer, the technical solutions in the embodiments of the present application are described clearly and completely below. Obviously, the described embodiments are part of the embodiments of the present application, not all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present application.

[0039] In the present application, the technical features described in an open manner include closed technical solutions composed of the listed features, and also include open technical solutions containing the listed features.

[0040] In this application, when it comes to numerical ranges, unless otherwise specified, the above numerical ranges are deemed to be continuous and include the minimum and maximum values ​​of the range, as well as each value between such minimum and maximum values. Further, when a range refers to an integer, each integer between the minimum and maximum values ​​of the range is included. In addition, when multiple ranges are provided to describe features or characteristics, the ranges can be merged. In other words, unless otherwise specified, all ranges disclosed herein should be understood to include any and all subranges included therein.

[0041] In the present application, there is no particular limitation on the specific dispersion and stirring treatment methods.

[0042] Unless otherwise specified, the components, raw materials or instruments used in the embodiments and comparative examples of the present invention are all commercially available raw materials or instruments, and the components and raw materials used in each parallel experiment are all of the same kind.

[0043] Among them, hydrolyzed lupin protein comes from Shanghai Boshuo Industrial.

[0044] Morus alba bark extract comes from Guangzhou Meiyi Biological.

[0045] Centella asiatica extract comes from Hunan Langlin Biology.

[0046] The following examples are provided to facilitate understanding of the present invention. These examples are not provided to limit the scope of the claims.

[0047] Example 1

[0048] A serum comprises the following components in percentage by weight: 4% of an anti-aging repair composition containing rose extracellular vesicles, 0.2% of ammonium acryloyldimethyltaurate / VP copolymer, 0.5% of 1,2-hexanediol, 0.5% of p-hydroxyacetophenone, 0.5% of tocopherol acetate, 2% of squalane, 4% of butylene glycol, and the balance of water.

[0049] The anti-aging repair composition containing rose extracellular vesicles comprises rose extracellular vesicles, sea fennel extract and hydrolyzed lupine protein; the mass ratio of the rose extracellular vesicles, sea fennel extract and hydrolyzed lupine protein is 1:1.5:1.5.

[0050] The preparation method of the sea fennel extract is:

[0051] The whole sea fennel herb is crushed into 100 meshes to obtain sea fennel powder, and the sea fennel powder, pectinase, cellulase, and hemicellulase are added to water, and enzymolysis is performed at 48°C for 5 hours to obtain an enzymolysate; the mass ratio of the sea fennel powder, pectinase, cellulase, hemicellulase, and water is 1:0.015:0.01:0.005:10.

[0052] The enzymatic hydrolysate is added to a low eutectic solvent, heated to 60°C, extracted under 300W ultrasound for 1 hour, centrifuged at 5000rpm for 10 minutes, and the supernatant is the sea fennel extract. The mass ratio of the enzymatic hydrolysate to the low eutectic solvent is 1:3; the low eutectic solvent includes betaine, allantoin, glycerol and water in a mass ratio of 1:1:3:1.5.

[0053] The method for preparing rose extracellular vesicles comprises the following steps:

[0054] (1) Preparation of rose extracellular vesicles: Rose and PBS buffer were added into a juicer at a solid-liquid ratio of 1 g:4 mL, mixed and juiced, and filtered through a 200-mesh filter cloth to obtain a filtrate;

[0055] Add cellulase, xylanase and β-glucanase to the filtrate, perform enzymolysis at 50°C for 5 hours, and filter through a 300-mesh filter cloth to obtain an enzymolysis solution; the mass ratio of the cellulase, xylanase, β-glucanase and filtrate is 0.025:0.018:0.012:1;

[0056] The enzymatic hydrolysate was added to an ultracentrifuge and centrifuged at 10,000 g for 30 min and then at 100,000 g for 60 min at 4°C. The solution was filtered through a 0.22 μm filter membrane, and the filtrate was resuspended in PBS buffer (the volume ratio of the filtrate to PBS was 1:10), and filtered through a 0.22 μm filter membrane to obtain rose extracellular vesicles.

[0057] The preparation method of the essence comprises the following steps: mixing water, butylene glycol, squalane, tocopherol acetate, and ammonium acryloyldimethyltaurate / VP copolymer at 65° C., cooling to 40° C., adding other materials, stirring evenly, discharging, and cooling to room temperature to obtain the essence.

[0058] Example 2

[0059] The difference between Example 2 and Example 1 is that the mass ratios of rose extracellular vesicles, sea fennel extract and hydrolyzed lupine protein are different, and the others are the same.

[0060] A serum comprises the following components in percentage by weight: 4% of an anti-aging repair composition containing rose extracellular vesicles, 0.2% of ammonium acryloyldimethyltaurate / VP copolymer, 0.5% of 1,2-hexanediol, 0.5% of p-hydroxyacetophenone, 0.5% of tocopherol acetate, 2% of squalane, 4% of butylene glycol, and the balance of water.

[0061] The anti-aging repair composition containing rose extracellular vesicles comprises rose extracellular vesicles, sea fennel extract and hydrolyzed lupine protein; the mass ratio of the rose extracellular vesicles, sea fennel extract and hydrolyzed lupine protein is 1:1.2:1.8.

[0062] Example 3

[0063] The difference between Example 3 and Example 1 is that the mass ratios of rose extracellular vesicles, sea fennel extract and hydrolyzed lupine protein are different, and the others are the same.

[0064] A serum comprises the following components in percentage by weight: 4% of an anti-aging repair composition containing rose extracellular vesicles, 0.2% of ammonium acryloyldimethyltaurate / VP copolymer, 0.5% of 1,2-hexanediol, 0.5% of p-hydroxyacetophenone, 0.5% of tocopherol acetate, 2% of squalane, 4% of butylene glycol, and the balance of water.

[0065] The anti-aging repair composition containing rose extracellular vesicles comprises rose extracellular vesicles, sea fennel extract and hydrolyzed lupine protein; the mass ratio of the rose extracellular vesicles, sea fennel extract and hydrolyzed lupine protein is 1:0.6:2.4.

[0066] Example 4

[0067] The difference between Example 4 and Example 1 is that the mass ratios of rose extracellular vesicles, sea fennel extract and hydrolyzed lupine protein are different, and the others are the same.

[0068] A serum comprises the following components in percentage by weight: 4% of an anti-aging repair composition containing rose extracellular vesicles, 0.2% of ammonium acryloyldimethyltaurate / VP copolymer, 0.5% of 1,2-hexanediol, 0.5% of p-hydroxyacetophenone, 0.5% of tocopherol acetate, 2% of squalane, 4% of butylene glycol, and the balance of water.

[0069] The anti-aging repair composition containing rose extracellular vesicles comprises rose extracellular vesicles, sea fennel extract and hydrolyzed lupine protein; the mass ratio of the rose extracellular vesicles, sea fennel extract and hydrolyzed lupine protein is 1:2.5:0.5.

[0070] Example 5

[0071] The difference between Example 5 and Example 1 is that the preparation parameters of the sea fennel extract are different, and the others are the same.

[0072] The preparation method of the sea fennel extract is:

[0073] The whole sea fennel herb is crushed into 100 meshes to obtain sea fennel powder, and the sea fennel powder, pectinase, cellulase, and hemicellulase are added to water, and enzymolysis is performed at 48°C for 5 hours to obtain an enzymolysate; the mass ratio of the sea fennel powder, pectinase, cellulase, hemicellulase, and water is 1:0.008:0.015:0.002:5.

[0074] The enzymatic hydrolysate is added to a low eutectic solvent, heated to 60°C, extracted under 300W ultrasound for 1 hour, centrifuged at 5000rpm for 10 minutes, and the supernatant is the sea fennel extract. The mass ratio of the enzymatic hydrolysate to the low eutectic solvent is 1:3; the low eutectic solvent includes betaine, allantoin, glycerol and water in a mass ratio of 1:0.5:4:0.5.

[0075] Example 6

[0076] The difference between Example 6 and Example 1 is that the preparation parameters of the sea fennel extract are different, and the others are the same.

[0077] The preparation method of the sea fennel extract is:

[0078] The whole sea fennel herb is crushed into 100 meshes to obtain sea fennel powder, and the sea fennel powder, pectinase, cellulase, and hemicellulase are added into water, and enzymolysis is performed at 48° C. for 5 hours to obtain an enzymolysate; the mass ratio of the sea fennel powder, pectinase, cellulase, hemicellulase, and water is 1:0.02:0.005:0.01:20.

[0079] The enzymatic hydrolysate is added to a low eutectic solvent, heated to 60°C, extracted under 300W ultrasound for 1 hour, centrifuged at 5000rpm for 10 minutes, and the supernatant is the sea fennel extract. The mass ratio of the enzymatic hydrolysate to the low eutectic solvent is 1:3; the low eutectic solvent includes betaine, allantoin, glycerol and water in a mass ratio of 1:1.2:2:2.

[0080] Comparative Example 1

[0081] The difference between Comparative Example 1 and Example 1 is that the mass ratios of rose extracellular vesicles, sea fennel extract and hydrolyzed lupin protein are different, and the others are the same.

[0082] A serum comprises the following components in percentage by weight: 4% of an anti-aging repair composition containing rose extracellular vesicles, 0.2% of ammonium acryloyldimethyltaurate / VP copolymer, 0.5% of 1,2-hexanediol, 0.5% of p-hydroxyacetophenone, 0.5% of tocopherol acetate, 2% of squalane, 4% of butylene glycol, and the balance of water.

[0083] The anti-aging repair composition containing rose extracellular vesicles comprises rose extracellular vesicles, sea fennel extract and hydrolyzed lupine protein; the mass ratio of the rose extracellular vesicles, sea fennel extract and hydrolyzed lupine protein is 1:3.8:0.2.

[0084] Comparative Example 2

[0085] The difference between Comparative Example 2 and Example 1 is that the mass ratios of rose extracellular vesicles, sea fennel extract and hydrolyzed lupin protein are different, and the others are the same.

[0086] A serum comprises the following components in percentage by weight: 4% of an anti-aging repair composition containing rose extracellular vesicles, 0.2% of ammonium acryloyldimethyltaurate / VP copolymer, 0.5% of 1,2-hexanediol, 0.5% of p-hydroxyacetophenone, 0.5% of tocopherol acetate, 2% of squalane, 4% of butylene glycol, and the balance of water.

[0087] The anti-aging repair composition containing rose extracellular vesicles comprises rose extracellular vesicles, sea fennel extract and hydrolyzed lupine protein; the mass ratio of the rose extracellular vesicles, sea fennel extract and hydrolyzed lupine protein is 1:0.2:3.8.

[0088] Comparative Example 3

[0089] The difference between Comparative Example 3 and Example 1 is that Comparative Example 3 is a single rose extracellular vesicle (the total amount remains unchanged), and the rest are the same.

[0090] A serum comprises the following components in percentage by weight: 4% rose extracellular vesicles, 0.2% ammonium acryloyldimethyltaurate / VP copolymer, 0.5% 1,2-hexanediol, 0.5% p-hydroxyacetophenone, 0.5% tocopherol acetate, 2% squalane, 4% butylene glycol, and the balance water.

[0091] Comparative Example 4

[0092] The difference between Comparative Example 4 and Example 1 is that Comparative Example 4 is a single sea fennel extract (the total amount remains unchanged), and the other parts are the same.

[0093] An essence comprises the following components in percentage by weight: 4% sea fennel extract, 0.2% ammonium acryloyldimethyltaurate / VP copolymer, 0.5% 1,2-hexanediol, 0.5% p-hydroxyacetophenone, 0.5% tocopherol acetate, 2% squalane, 4% butylene glycol, and the balance water.

[0094] Comparative Example 5

[0095] The difference between Comparative Example 5 and Example 1 is that Comparative Example 5 is a single hydrolyzed lupin protein (the total amount remains unchanged), and the other contents are the same.

[0096] An essence comprises the following components in percentage by weight: 4% sea fennel extract, 0.2% ammonium acryloyldimethyltaurate / VP copolymer, 0.5% 1,2-hexanediol, 0.5% p-hydroxyacetophenone, 0.5% tocopherol acetate, 2% squalane, 4% butylene glycol, and the balance water.

[0097] Comparative Example 6

[0098] The difference between Comparative Example 6 and Example 1 is that Comparative Example 6 does not contain rose extracellular vesicles (the total amount remains unchanged), and the other parts are the same.

[0099] A serum comprises the following components in percentage by weight: 4% anti-aging repair composition, 0.2% ammonium acryloyldimethyltaurate / VP copolymer, 0.5% 1,2-hexanediol, 0.5% p-hydroxyacetophenone, 0.5% tocopherol acetate, 2% squalane, 4% butylene glycol, and the balance water.

[0100] The anti-aging repair composition comprises sea fennel extract and hydrolyzed lupine protein; the mass ratio of the sea fennel extract to the hydrolyzed lupine protein is 1.5:1.5.

[0101] Comparative Example 7

[0102] The difference between Comparative Example 7 and Example 1 is that Comparative Example 7 does not contain sea fennel extract (the total amount remains unchanged), and the other contents are the same.

[0103] A serum comprises the following components in percentage by weight: 4% of an anti-aging repair composition containing rose extracellular vesicles, 0.2% of ammonium acryloyldimethyltaurate / VP copolymer, 0.5% of 1,2-hexanediol, 0.5% of p-hydroxyacetophenone, 0.5% of tocopherol acetate, 2% of squalane, 4% of butylene glycol, and the balance of water.

[0104] The anti-aging repair composition containing rose extracellular vesicles comprises rose extracellular vesicles and hydrolyzed lupine protein; the mass ratio of the rose extracellular vesicles to the hydrolyzed lupine protein is 1:1.5.

[0105] Comparative Example 8

[0106] The difference between Comparative Example 8 and Example 1 is that Comparative Example 8 does not contain hydrolyzed lupin protein (the total amount remains unchanged), and the other contents are the same.

[0107] A serum comprises the following components in percentage by weight: 4% of an anti-aging repair composition containing rose extracellular vesicles, 0.2% of ammonium acryloyldimethyltaurate / VP copolymer, 0.5% of 1,2-hexanediol, 0.5% of p-hydroxyacetophenone, 0.5% of tocopherol acetate, 2% of squalane, 4% of butylene glycol, and the balance of water.

[0108] The anti-aging repair composition containing rose extracellular vesicles comprises rose extracellular vesicles and sea fennel extract; the mass ratio of the rose extracellular vesicles to the sea fennel extract is 1:1.5.

[0109] Comparative Example 9

[0110] The difference between Comparative Example 9 and Example 1 is that Comparative Example 9 uses Morus alba bark extract to replace Crimson Fennel extract, and the other contents are the same.

[0111] A serum comprises the following components in percentage by weight: 4% of an anti-aging repair composition containing rose extracellular vesicles, 0.2% of ammonium acryloyldimethyltaurate / VP copolymer, 0.5% of 1,2-hexanediol, 0.5% of p-hydroxyacetophenone, 0.5% of tocopherol acetate, 2% of squalane, 4% of butylene glycol, and the balance of water.

[0112] The anti-aging repair composition containing rose extracellular vesicles comprises rose extracellular vesicles, mulberry bark extract and hydrolyzed lupine protein; the mass ratio of the rose extracellular vesicles, mulberry bark extract and hydrolyzed lupine protein is 1:1.5:1.5.

[0113] Comparative Example 10

[0114] The difference between Comparative Example 10 and Example 1 is that Comparative Example 10 uses Centella asiatica extract to replace hydrolyzed lupin protein, and the rest is the same.

[0115] A serum comprises the following components in percentage by weight: 4% of an anti-aging repair composition containing rose extracellular vesicles, 0.2% of ammonium acryloyldimethyltaurate / VP copolymer, 0.5% of 1,2-hexanediol, 0.5% of p-hydroxyacetophenone, 0.5% of tocopherol acetate, 2% of squalane, 4% of butylene glycol, and the balance of water.

[0116] The anti-aging repair composition containing rose extracellular vesicles comprises rose extracellular vesicles, sea fennel extract and Centella asiatica extract; the mass ratio of the rose extracellular vesicles, sea fennel extract and Centella asiatica extract is 1:1.5:1.5.

[0117] Comparative Example 11

[0118] The difference between Comparative Example 11 and Example 1 is that the preparation method of the sea fennel extract is different, and the other aspects are the same.

[0119] The preparation method of the sea fennel extract is:

[0120] The whole sea fennel herb is crushed into 100 meshes to obtain sea fennel powder, and the sea fennel powder, pectinase, cellulase and hemicellulase are added to water, and enzymolysis is performed at 48°C for 5 hours to obtain an enzymolysis product, which is filtered and dried to obtain a sea fennel extract; the mass ratio of the sea fennel powder, pectinase, cellulase, hemicellulase and water is 1:0.015:0.01:0.005:10.

[0121] Comparative Example 12

[0122] The difference between Comparative Example 12 and Example 1 is that the preparation method of the sea fennel extract is different, and the other aspects are the same.

[0123] The whole herb of sea fennel is crushed into 100 meshes to obtain sea fennel powder, and the sea fennel powder is added into water (each time the solid-liquid ratio is 1 g: 10 mL), refluxed and extracted twice, and the extracts are combined, filtered, and dried to obtain the sea fennel extract.

[0124] Test Case

[0125] 1. Irritation test: The test was conducted in accordance with the method of SNT 2329-2009, and the results were evaluated using the irritation scoring method.

[0126] The specific scores are shown in Table 1, and the evaluation results are shown in Table 2.

[0127] Table 1

[0128] Stimulus score Irritation classification IS<1 Non-irritating 1≤IS<5 Mild irritation 5≤IS<9 Moderate irritation IS ≥ 10 Strong irritant / corrosive

[0129] Table 2

[0130] Experimental Group Stimulus score IS Irritation classification Example 1 ＜1 Non-irritating Example 2 ＜1 Non-irritating Example 3 ＜1 Non-irritating Example 4 ＜1 Non-irritating Example 5 ＜1 Non-irritating Example 6 ＜1 Non-irritating Comparative Example 1 ＜1 Non-irritating Comparative Example 2 ＜1 Non-irritating Comparative Example 3 ＜1 Non-irritating Comparative Example 4 ＜1 Non-irritating Comparative Example 5 ＜1 Non-irritating Comparative Example 6 ＜1 Non-irritating Comparative Example 7 ＜1 Non-irritating Comparative Example 8 ＜1 Non-irritating Comparative Example 9 ＜1 Non-irritating Comparative Example 10 ＜1 Non-irritating Comparative Example 11 ＜1 Non-irritating Comparative Example 12 ＜1 Non-irritating

[0131] It can be seen from Table 2 that the essence containing the anti-aging repair composition of the present invention is mild and non-irritating and has good safety.

[0132] 2. Stability test

[0133] Weigh 9 samples of 20 g of the essence of the embodiment and the comparative example in transparent PET bottles (3 test samples at each temperature), place them at constant temperatures of -18°C, 25°C, and 48°C for 30 days, and observe the test samples after returning to room temperature. If there is no stratification or oil release, and no change in transparency, it is considered normal. Otherwise, it is considered abnormal.

[0134] Table 3

[0135] Experimental Group -18℃ 25℃ 48℃ Example 1 No abnormality No abnormality No abnormality Example 2 No abnormality No abnormality No abnormality Example 3 No abnormality No abnormality No abnormality Example 4 No abnormality No abnormality No abnormality Example 5 No abnormality No abnormality No abnormality Example 6 No abnormality No abnormality No abnormality Comparative Example 1 No abnormality No abnormality No abnormality Comparative Example 2 No abnormality No abnormality No abnormality Comparative Example 3 No abnormality No abnormality No abnormality Comparative Example 4 No abnormality No abnormality No abnormality Comparative Example 5 No abnormality No abnormality No abnormality Comparative Example 6 No abnormality No abnormality No abnormality Comparative Example 7 No abnormality No abnormality No abnormality Comparative Example 8 No abnormality No abnormality No abnormality Comparative Example 9 No abnormality No abnormality No abnormality Comparative Example 10 No abnormality No abnormality No abnormality Comparative Example 11 No abnormality No abnormality No abnormality Comparative Example 12 No abnormality No abnormality No abnormality

[0136] It can be seen from Table 3 that the essence containing the anti-aging repair composition of the present invention has excellent stability.

[0137] 3. Efficacy test

[0138] 108 volunteers were selected and all volunteers were randomly divided into 18 groups with 6 people in each group. The volunteers in each group tried the essence of the embodiment and the comparative example respectively. After cleansing their faces in the morning and evening every day, the volunteers used the above products on their entire faces for 30 consecutive days. The skin hydration, transepidermal water loss (TEWL), skin wrinkle value and skin elasticity value were tested on the 0th day and the 30th day of the trial samples, and the skin hydration change rate, transepidermal water loss (TEWL) change rate, skin wrinkle change rate and skin elasticity change rate were calculated.

[0139] The criteria for inclusion of volunteers are: aged 25 to 35 years, female; those who have not used hormone drugs and immunosuppressants in the past month; those who have not participated in other clinical trials at present or in the past 30 days; those who can cooperate well with the trial; those who can read and understand all the contents of the informed consent form and voluntarily sign the informed consent form; and those who agree not to use any cosmetics, drugs, and health products that may affect the results during the trial.

[0140] Exclusion criteria: those with open wounds or eroded surfaces; those with highly allergic constitutions; those with a history of cosmetic allergies; women who are pregnant, breastfeeding, or planning to become pregnant during the test period; those with severe heart, liver, and kidney damage and severe immunodeficiency; those with mental illness, severe endocrine diseases, and those taking oral contraceptives; those who have participated in drug clinical trials or other trials within 30 days, or those who have systemically used drugs that may affect the test results within the past week; those who have taken oral or external beauty products that may affect the test results within 2 weeks; those who are considered by the researchers to be unsuitable for participating in this test.

[0141] Suspension and exit criteria: Failure to apply the test sample according to the test requirements or failure to make follow-up records as required during the trial; serious illness or serious adverse reactions during use.

[0142] The skin hydration was tested by Corneometer CM 825, the transepidermal water loss was tested by TewameterTM 300, and the skin wrinkle value and skin elasticity value were tested by PRIMOS, a rapid three-dimensional skin imaging system.

[0143] Skin hydration change rate = [(skin hydration on day 30 - skin hydration on day 0) / skin hydration on day 0] * 100%.

[0144] Transdermal water loss change rate = [(transdermal water loss on the 30th day - transdermal water loss on the 0th day) / transdermal water loss degree on the 0th day] * 100%.

[0145] Skin wrinkle change rate = [(skin wrinkle value on day 30 - skin wrinkle value on day 0) / skin wrinkle value on day 0] * 100%.

[0146] Skin elasticity change rate = [(skin elasticity value on day 30 - skin elasticity value on day 0) / skin elasticity value on day 0] * 100%.

[0147] Table 4

[0148]

[0149]

[0150] From Table 4, the anti-aging and repairing composition containing rose extracellular vesicles of the present invention can significantly improve skin hydration and skin elasticity, reduce transepidermal water loss and wrinkle values, and has excellent anti-aging and repairing effects.

[0151] By comparing Examples 1 to 4 with Comparative Examples 1 to 2, it can be seen that the anti-aging and repairing effect can be significantly improved by controlling the mass ratio of the rose extracellular vesicles, the sea fennel extract and the hydrolyzed lupin protein to 1: (0.6 to 2.5): (0.5 to 2.4).

[0152] By comparing Examples 1 to 2 with Examples 3 to 4, it can be seen that the anti-aging and repairing effect can be further improved by controlling the mass ratio of the rose extracellular vesicles, the sea fennel extract and the hydrolyzed lupin protein to 1:(1.2-1.5):(1.5-1.8).

[0153] By comparing Example 1 with Comparative Examples 3 to 10, it can be seen that the rose extracellular vesicles, sea fennel extract and hydrolyzed lupin protein described in the present invention have a significant synergistic effect in anti-aging and repair. Under the mutual influence and interaction of the three, the anti-aging and repair effect is significantly improved. When the total amount remains unchanged, the lack of any one of them or the use of other ingredients to replace it will lead to a significant decrease in the anti-aging and repair effect.

[0154] By comparing Example 1 with Comparative Examples 11 to 12, it can be seen that the sea fennel extract prepared by the method described in the present invention can significantly improve the anti-aging and repair effect.

[0155] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solution of the present invention can be modified or replaced by equivalents without departing from the essence and scope of the technical solution of the present invention.

Claims

1. An anti-aging repair composition containing rose extracellular vesicles, characterized in that: The invention comprises rose extracellular vesicles, sea fennel extract and hydrolyzed lupine protein; the mass ratio of the rose extracellular vesicles, sea fennel extract and hydrolyzed lupine protein is 1:(0.6-2.5):(0.5-2.4).

2. The anti-aging and repairing composition containing rose extracellular vesicles according to claim 1, characterized in that: The mass ratio of the rose extracellular vesicles, the sea fennel extract and the hydrolyzed lupin protein is 1:(1.2-1.5):(1.5-1.8).

3. The anti-aging and repairing composition containing rose extracellular vesicles according to claim 1, characterized in that: The preparation method of the sea fennel extract is: The whole sea fennel herb is crushed to obtain sea fennel powder, and the sea fennel powder, pectinase, cellulase, and hemicellulase are added into water for enzymolysis to obtain an enzymolysate; The enzymatic hydrolysate is added into a low eutectic solvent, subjected to ultrasonic extraction and centrifugation to obtain the sea fennel extract.

4. The anti-aging and repairing composition containing rose extracellular vesicles according to claim 3, characterized in that: The mass ratio of the sea fennel powder, pectinase, cellulase, hemicellulase and water is 1: (0.008-0.02): (0.005-0.015): (0.002-0.01): (5-20).

5. The anti-aging and repairing composition containing rose extracellular vesicles according to claim 3, characterized in that: The enzymolysis temperature is 42-52° C., and the enzymolysis time is 1-8 hours.

6. The anti-aging and repairing composition containing rose extracellular vesicles according to claim 3, characterized in that: The low eutectic solvent comprises betaine, allantoin, glycerol and water in a mass ratio of 1:(0.5-1.2):(2-4):(0.5-2).

7. The anti-aging and repairing composition containing rose extracellular vesicles according to claim 3, characterized in that: The power of the ultrasonic extraction is 200-500W, the time is 0.5-2h, and the temperature is 45-65°C.

8. Use of the anti-aging and repairing composition containing rose extracellular vesicles according to any one of claims 1 to 7 in the preparation of cosmetics.

9. The use according to claim 8, characterized in that: The dosage form of the cosmetic is water solution, emulsion, cream, gel or facial mask.

10. An essence, characterized in that: The invention comprises the following components in percentage by weight: 0.1-10% of an anti-aging repair composition containing rose extracellular vesicles, 0.1-0.5% of ammonium acryloyldimethyltaurate / VP copolymer, 0.2-0.8% of 1,2-hexanediol, 0.2-0.8% of p-hydroxyacetophenone, 0.2-1% of tocopherol acetate, 1-4% of squalane, 2-5% of butanediol, and 77.9-96.2% of water; The anti-aging and repairing composition containing rose extracellular vesicles is the anti-aging and repairing composition containing rose extracellular vesicles according to any one of claims 1 to 7.

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