Preparation method of mulberry-ginger extract

Abstract

The invention relates to a preparation method and application of a mulberry-ginger extract. The mulberry-ginger extract is prepared from 30 parts of mulberry leaves, 16 parts of fructus forsythiae, 12 parts of chrysanthemum, 10 parts of dried ginger, 16 parts of semen armeniacae amarae and 16 parts of perilla leaves. The invention provides a mulberry-ginger extract preparation process capable of shortening the production period and improving the transfer rate of effective components.

Description

Technical Field

[0001] The invention belongs to the field of traditional Chinese medicine preparations, and particularly relates to a method for preparing mulberry and ginger extract. Background Art

[0002] Usually, the preparation process of Chinese medicine extracts adopts the water extraction and alcohol precipitation method. However, in the long-term use process, many problems are found: first, it is impossible to extract all the effective ingredients of each medicine in the whole prescription during water extraction, resulting in a decrease in content, thereby affecting the clinical efficacy of Chinese medicine preparations. Second, the production cost is high, and the amount of alcohol consumed in the ethanol recovery process is large. Third, the finished product has many impurities, and there are still many impurities in the precipitation process, especially proteins, which will bring unexpected adverse reactions to the injection when used, causing pain and loss to patients.

[0003] Cold, also known as upper respiratory tract infection, is a general term for acute inflammation of the nasal cavity, pharynx or larynx. In a broad sense, upper respiratory tract infection is not a disease diagnosis, but a group of diseases, including common cold, viral pharyngitis, laryngitis, herpetic pharyngitis, pharyngoconjunctival fever, and bacterial pharyngitis-tonsillitis. In a narrow sense, upper respiratory tract infection, also known as common cold, is the most common acute respiratory infectious disease, which is mostly self-limiting, but the incidence is high. It occurs 2 to 4 times a year in adults, and the incidence in children is higher, 6 to 8 times a year. It can occur throughout the year, but more often in winter and spring. Mulberry and ginger cold injection is prepared from six traditional Chinese medicines: mulberry leaves, chrysanthemums, forsythia, bitter almonds, dried ginger, and perilla. It has the effects of dispersing wind and heat, removing phlegm and relieving cough. It is mainly used to treat colds, coughs, headaches, and sore throats. Summary of the invention

[0004] Ultra-high pressure extraction (UHPE), also known as ultra-cold isostatic extraction, refers to the use of 100-1000MPa of fluid static pressure on the mixture of extraction solvent and traditional Chinese medicine at room temperature, and maintaining it at a predetermined pressure for a period of time, so that the pressure inside and outside the plant cells reaches equilibrium and then the pressure is quickly released. Due to the sudden increase in osmotic pressure inside and outside the cells, the cell membrane structure changes, allowing the active ingredients in the cells to penetrate various cell membranes and transfer to the extract outside the cells, achieving the purpose of extracting the active ingredients of traditional Chinese medicine. Ultra-high pressure extraction technology has the advantages of high extraction efficiency, ability to retain the biological activity of the extract, low energy consumption, wide range of applications, simple and safe process operation, etc. in traditional Chinese medicine extraction.

[0005] Adsorption clarification is a high-tech method that adds an adsorption clarifier to the Chinese medicine extract or extract concentrate to remove coarse particles in the solution by adsorption, so as to achieve the purpose of refining and improving the clarity of the preparation. The adsorption clarifier used is a substance that flocculates and precipitates large particles and suspended particles with Stoke precipitation tendency in the system through adsorption, bridging, flocculation, and coagulation of inorganic salt electrolyte particles and surface charges, while retaining most effective high-molecular substances such as polysaccharides, and utilizing the protective effect of high-molecular natural hydrophilic colloids on hydrophobic colloids, thereby improving the stability and clarity of the preparation. Using a clarifier to adsorb and clarify the Chinese medicine extract has the advantages of less loss of effective ingredients, high clarification efficiency, simple operation, less time consumption, low cost, safety and non-toxicity, and no residue. Common natural clarifiers include chitosan, juice 101, and ZTC1+1Ⅱ. In order to overcome the problems of existing mulberry and ginger cold preparations, the inventors conducted a large number of experimental studies and found an efficient extraction method and an efficient purification method. At present, there is no report on the preparation of mulberry and ginger extract by combining ultra-high pressure extraction and adsorption clarification.

[0006] At present, there is no report on the preparation of Chinese medicine composition extract, especially mulberry ginger extract, by ultra-high pressure extraction or natural adsorption clarification.

[0007] The invention aims to provide a method for preparing mulberry and ginger extract.

[0008] At the same time, the purpose of the present invention is also to provide a mulberry and ginger extract.

[0009] Furthermore, the present invention also aims to provide an application of mulberry and ginger extract in a traditional Chinese medicine compound preparation.

[0010] The preparation process of the mulberry and ginger extract of the present invention comprises the following steps:

[0011] (1) Take mulberry leaves, forsythia suspensa, and chrysanthemum in the proportion of mulberry and ginger as combination I, and take dried ginger, bitter almonds, and perilla leaves in the proportion of mulberry and ginger as combination II.

[0012] (2) respectively crushing the combination I and II, adding 4-5 times the weight of the medicinal materials to the crushed I and II, respectively, and soaking for 2-5 hours;

[0013] (3) the wet medicinal material obtained in step (2) and its soaking solution are placed in special containers, respectively, and ultrahigh pressure extraction is performed on each container, and finally the two extracts are combined;

[0014] (4) adding a clarifier to the combined extract obtained in step (3), stirring evenly, maintaining the temperature at 35-65° C., standing for 4-5 hours, centrifuging at high speed, and taking the supernatant;

[0015] (5) performing microfiltration on the supernatant obtained in step (4) to obtain a mulberry ginger filtrate;

[0016] (6) concentrating the mulberry and ginger filtrate obtained in step (5) to obtain a mulberry and ginger extract;

[0017] The mulberry and ginger formula described in step (1) comprises 30 parts of mulberry leaves, 16 parts of forsythia, 12 parts of chrysanthemum, 10 parts of dried ginger, 16 parts of bitter almonds and 16 parts of perilla leaves.

[0018] The amount of water added in step (1) is preferably 3-5 times the total weight of the medicinal materials.

[0019] The ultra-high pressure extraction in step (3) has a pressure of 400-900 MPa, preferably 500 MPa.

[0020] The ultra-high pressure extraction in step (3) is performed 3-6 times, preferably 2-4 times.

[0021] The ultra-high pressure extraction in step (3) has an extraction time of 18-30 minutes.

[0022] The natural clarifier described in step (4) is one of chitosan, fruit juice 101 and / or ZTC1+1Ⅱ, preferably ZTC1+1Ⅱ.

[0023] The natural clarifier described in step (4) is ZTC1+1Ⅱ, wherein the order of adding components A and B is B first and then A.

[0024] The natural clarifier described in step (4) is ZTC1+1Ⅱ. Components A and B are prepared into 1% A and B solutions respectively with 1% acetic acid according to the instructions.

[0025] The natural clarifier described in step (4) is ZTC1+1Ⅱ, and the addition amounts of components A and B are 2-10% and 4-20% of the volume of the liquid respectively.

[0026] The standing time in step (4) is 3-8 hours, preferably 6-8 hours.

[0027] The pore size of the microporous filtration described in step (5) is 1.2 μm.

[0028] According to production needs, the prepared mulberry ginger extract is mixed with appropriate auxiliary materials and then subjected to corresponding process to obtain mulberry ginger tablets, mulberry ginger capsules, mulberry ginger granules, mulberry ginger oral liquid and other preparations.

[0029] The present invention adopts an ultra-high pressure extraction method to extract dried ginger, perilla, bitter almonds containing colds and colds and mulberry leaves, forsythia, and chrysanthemum containing colds and colds, respectively, and can efficiently extract effective ingredients for treating colds and colds from the two groups of medicinal materials. In the process of removing impurities, an adsorption clarification technology is used to add a natural clarifier to the extract, and finally the extract is further removed by microporous filtration technology, which ensures the efficient removal of impurities and the quality of the effective ingredients in the extract. The process of preparing mulberry and ginger extract by combining ultra-high pressure extraction and adsorption clarification technology simplifies the production process, shortens the production time, replaces the traditional ethanol impurity removal with a natural clarifier, saves costs, and avoids environmental pollution caused by ethanol. The mulberry and ginger preparation prepared by the mulberry and ginger extract prepared by the production process has a good market prospect. DETAILED DESCRIPTION

[0030] Example 1

[0031] (1) According to the mulberry and ginger formula ratio, 30 kg of mulberry leaves, 16 kg of forsythia, and 12 kg of chrysanthemum were weighed as combination I, and 10 kg of dried ginger, 16 kg of bitter almonds, and 16 kg of perilla leaves were weighed as combination II;

[0032] (2) respectively crushing the combination I and II, and then adding 4 times the weight of the medicinal materials of the crushed combination I and II into the crushed combination I and II, respectively, and soaking for 3 hours;

[0033] (3) placing the wet medicinal materials of combination I and II obtained in step (2) together with their soaking liquid into an ultra-high pressure extractor, extracting them 5 times at 200 MPa for 20 minutes each time, combining the four extracts, and finally combining the extracts of combination I and II;

[0034] (4) Adding 4% of the volume of the natural clarifier ZTC1+1Ⅱ (manufacturer: Beijing Zhengtiancheng Clarification Technology Co., Ltd.) component B solution to the extract obtained in step (3), stirring evenly, after the temperature of the liquid rises to 65° C., adding 2% of the volume of the liquid ZTC1+1Ⅱ (Beijing Zhengtiancheng Clarification Technology Co., Ltd.) component A solution, stirring evenly, standing for 8 hours, high-speed centrifugation, and taking the supernatant;

[0035] (5) filtering the supernatant obtained in step (4) through a 1.2 μm micropore filter to obtain a mulberry ginger filtrate;

[0036] (6) concentrating the mulberry and ginger filtrate obtained in step (5) to obtain a mulberry and ginger extract;

[0037] (7) According to production needs, the mulberry ginger extract obtained in step (6) is used with appropriate auxiliary materials and then subjected to corresponding process to prepare mulberry ginger tablets, mulberry ginger capsules, mulberry ginger granules, mulberry ginger oral liquid and other preparations.

[0038] Example 2

[0039] (1) According to the mulberry and ginger formula ratio, 15 kg of mulberry leaves, 8 kg of forsythia, and 6 kg of chrysanthemum were weighed as combination I, and 5 kg of dried ginger, 8 kg of bitter almonds, and 8 kg of perilla leaves were weighed as combination II;

[0040] (2) respectively crushing the combination I and II, and then adding 4 times the weight of the medicinal materials of the crushed combination I and II into the crushed combination I and II, respectively, and soaking for 4 hours;

[0041] (3) placing the wet medicinal materials of combination I and II obtained in step (2) together with their soaking liquid into an ultra-high pressure extractor, extracting them once at 800 MPa for 5 minutes, and combining the extracts of combination I and II;

[0042] (4) adding 20% ​​of the volume of the natural clarifier ZTC1+1Ⅱ component B solution to the extract obtained in step (3), stirring evenly, and after the temperature of the solution rises to 85° C., adding 10% of the volume of the ZTC1+1Ⅱ component A solution, stirring evenly, standing for 1 hour, and centrifuging at high speed to obtain the supernatant;

[0043] (5) filtering the supernatant obtained in step (4) through a 1.2 μm micropore filter to obtain a mulberry ginger filtrate;

[0044] (6) concentrating the mulberry and ginger filtrate obtained in step (5) to obtain a mulberry and ginger extract;

[0045] (7) According to production needs, the mulberry ginger extract obtained in step (6) is used with appropriate auxiliary materials and then subjected to corresponding process to prepare mulberry ginger tablets, mulberry ginger capsules, mulberry ginger granules, mulberry ginger oral liquid, mulberry ginger injection and other preparations.

[0046] Example 3

[0047] (1) According to the mulberry and ginger formula ratio, 15 kg of mulberry leaves, 8 kg of forsythia, and 6 kg of chrysanthemum were weighed as combination I, and 5 kg of dried ginger, 8 kg of bitter almonds, and 8 kg of perilla leaves were weighed as combination II;

[0048] (2) respectively crushing the combination I and II, and then adding 6 times the weight of the medicinal materials of the crushed combination I and II into the crushed combination I and II, respectively, and soaking for 1 hour;

[0049] (3) placing the wet medicinal materials of combination I and II obtained in step (2) together with their soaking liquid into an ultra-high pressure extractor, extracting them twice at 400 MPa for 8 minutes each time, combining the two extracts, and finally combining the extracts of combination I and II;

[0050] (4) adding 8% of the volume of the natural clarifier ZTC1+1Ⅱ component B solution to the extract obtained in step (3), stirring evenly, and after the temperature of the solution rises to 70° C., adding 4% of the volume of the ZTC1+1Ⅱ component A solution, stirring evenly, standing for 3 hours, and then centrifuging at high speed to obtain the supernatant;

[0051] (5) filtering the supernatant obtained in step (4) through a 1.2 μm micropore filter to obtain a mulberry ginger filtrate;

[0052] (6) concentrating the mulberry and ginger filtrate obtained in step (5) to obtain a mulberry and ginger extract;

[0053] (7) According to production needs, the mulberry ginger extract obtained in step (6) is used with appropriate auxiliary materials and then subjected to corresponding process to prepare mulberry ginger tablets, mulberry ginger granules, mulberry ginger capsules, mulberry ginger oral liquid and other preparations.

[0054] Example 4

[0055] (1) According to the mulberry and ginger formula ratio, 30 kg of mulberry leaves, 16 kg of forsythia, and 12 kg of chrysanthemum were weighed as combination I, and 10 kg of dried ginger, 16 kg of bitter almonds, and 16 kg of perilla leaves were weighed as combination II;

[0056] (2) respectively crushing the combination I and II, and then adding 5 times the weight of the medicinal materials of the crushed combination I and II into the crushed combination I and II, respectively, and soaking for 2 hours;

[0057] (3) placing the wet medicinal materials of combination I and II obtained in step (2) together with their soaking liquid into an ultra-high pressure extractor, extracting them once at 600 MPa for 15 minutes, and finally combining the extracts of combination I and II;

[0058] (4) adding 10% of the volume of the natural clarifier ZTC1+1Ⅱ component B solution to the extract obtained in step (3), stirring evenly, maintaining the temperature of the solution at 25° C., adding 5% of the volume of the ZTC1+1Ⅱ component A solution, stirring evenly, standing for 8 hours, and centrifuging at high speed to obtain the supernatant;

[0059] (5) filtering the supernatant obtained in step (4) through a 1.2 μm micropore filter to obtain a mulberry ginger filtrate;

[0060] (6) concentrating the mulberry and ginger filtrate obtained in step (5) to obtain a mulberry and ginger extract;

[0061] (7) According to production needs, the mulberry ginger extract obtained in step (6) is used with appropriate auxiliary materials and then subjected to corresponding process to prepare mulberry ginger tablets, mulberry ginger capsules, mulberry ginger granules, mulberry ginger oral liquid and other preparations.

[0062] Example 5

[0063] (1) According to the mulberry and ginger formula ratio, 15 kg of mulberry leaves, 8 kg of forsythia, and 6 kg of chrysanthemum were weighed as combination I, and 5 kg of dried ginger, 8 kg of bitter almonds, and 8 kg of perilla leaves were weighed as combination II;

[0064] (2) respectively crushing the combination I and II, and then adding 3 times the weight of the medicinal materials of the crushed combination I and II into water, respectively, and soaking for 2 hours;

[0065] (3) placing the wet medicinal materials of combination I and II obtained in step (2) together with their soaking liquid into an ultra-high pressure extractor, extracting them three times at 300 MPa for 10 minutes each time, combining the three extracts, and finally combining the extracts of combination I and II;

[0066] (4) adding 16% of the volume of the natural clarifier ZTC1+1Ⅱ component B solution to the extract obtained in step (3), stirring evenly, and after the temperature of the solution rises to 50° C., adding 8% of the volume of the ZTC1+1Ⅱ component A solution, stirring evenly, standing for 6 hours, and centrifuging at high speed to obtain the supernatant;

[0067] (5) filtering the supernatant obtained in step (4) through a 1.2 μm micropore filter to obtain a mulberry ginger filtrate;

[0068] (6) concentrating the mulberry and ginger filtrate obtained in step (5) to obtain a mulberry and ginger extract;

[0069] (7) According to production needs, the mulberry ginger extract obtained in step (6) is used with appropriate auxiliary materials and then subjected to corresponding process to prepare mulberry ginger tablets, mulberry ginger granules, mulberry ginger capsules, mulberry ginger oral liquid and other preparations.

[0070] Example 6

[0071] (1) According to the mulberry and ginger formula ratio, 30 kg of mulberry leaves, 16 kg of forsythia, and 12 kg of chrysanthemum were weighed as combination I, and 10 kg of dried ginger, 16 kg of bitter almonds, and 16 kg of perilla leaves were weighed as combination II;

[0072] (2) respectively crushing the combination I and II, and then adding 4 times the weight of the medicinal materials of the crushed combination I and II into the crushed combination I and II, respectively, and soaking for 2 hours;

[0073] (3) placing the wet medicinal materials of combination I and II obtained in step (2) together with their soaking liquid into an ultra-high pressure extractor, extracting them three times at 400 MPa for 7 minutes each time, combining the three extracts, and finally combining the extracts of combination I and II;

[0074] (4) adding 10% of the volume of the natural clarifier ZTC1+1Ⅱ component B solution to the extract obtained in step (3), stirring evenly, and after the temperature of the solution rises to 85° C., adding 5% of the volume of the ZTC1+1Ⅱ component A solution, stirring evenly, standing for 7 hours, and centrifuging at high speed to obtain the supernatant;

[0075] (5) filtering the supernatant obtained in step (4) through a 1.2 μm micropore filter to obtain a mulberry ginger filtrate;

[0076] (6) concentrating the mulberry and ginger filtrate obtained in step (5) to obtain a mulberry and ginger extract;

[0077] (7) According to production needs, the mulberry ginger extract obtained in step (6) is used with appropriate auxiliary materials and then subjected to corresponding process to prepare mulberry ginger tablets, mulberry ginger granules, mulberry ginger capsules, mulberry ginger oral liquid and other preparations.

[0078] Example 7

[0079] (1) According to the mulberry and ginger formula ratio, 30 kg of mulberry leaves, 16 kg of forsythia, and 12 kg of chrysanthemum were weighed as combination I, and 10 kg of dried ginger, 16 kg of bitter almonds, and 16 kg of perilla leaves were weighed as combination II;

[0080] (2) respectively crushing the combination I and II, and then adding 4 times the weight of the medicinal materials of the crushed combination I and II into the crushed combination I and II, respectively, and soaking for 2 hours;

[0081] (3) placing the wet medicinal materials of combination I and II obtained in step (2) together with their soaking liquid into an ultra-high pressure extractor, extracting them three times at 400 MPa for 8 minutes each time, combining the three extracts, and finally combining the extracts of combination I and II;

[0082] (4) Slightly concentrate the extract obtained in step (3), add 95% ethanol to the concentrated extract to adjust the ethanol concentration in the extract to 65%-75%, stir, let stand for 10-24 hours, centrifuge, and take the supernatant.

[0083] (5) concentrating the supernatant obtained in step (4) to recover ethanol to obtain mulberry and ginger extract;

[0084] (6) According to production needs, the mulberry ginger extract obtained in step (5) is used with appropriate auxiliary materials and then subjected to corresponding process to prepare mulberry ginger tablets, mulberry ginger granules, mulberry ginger capsules, mulberry ginger oral liquid and other preparations.

[0085] Example 8

[0086] (1) according to the mulberry and ginger formula ratio, weigh 30 kg of mulberry leaves, 16 kg of forsythia, 12 kg of chrysanthemum, 10 kg of dried ginger, 16 kg of bitter almonds, and 16 kg of perilla leaves, grind the six medicinal materials, add 4 times the weight of the total medicinal materials in water, soak for 1-3 hours, and then extract the effective ingredients in a traditional Chinese medicine extraction tank for 6-8 hours, and filter to obtain a mulberry and ginger water extract;

[0087] (2) adding 10% of the volume of the natural clarifier ZTC1+1Ⅱ component B solution to the mulberry ginger water extract obtained in step (1), stirring evenly, and after the temperature of the liquid rises to 75° C., adding 5% of the volume of the ZTC1+1Ⅱ component A solution, stirring evenly, standing for 7 hours, and centrifuging at high speed to obtain the supernatant;

[0088] (3) filtering the supernatant obtained in step (2) through a 1.2 μm micropore filter to obtain a mulberry ginger filtrate;

[0089] (4) concentrating the mulberry and ginger filtrate obtained in step (3) to obtain a mulberry and ginger extract;

[0090] (5) According to production needs, the mulberry ginger extract obtained in step (4) is used with appropriate auxiliary materials and then subjected to corresponding process to prepare mulberry ginger tablets, mulberry ginger granules, mulberry ginger capsules, mulberry ginger oral liquid and other preparations.

[0091] Reference Example 1

[0092] (1) According to the formula ratio of mulberry and ginger, weigh 15 kg of mulberry leaves (the remaining 15 kg of mulberry leaves are reserved), 16 kg of forsythia, 12 kg of chrysanthemum, 10 kg of dried ginger, 16 kg of bitter almonds, and 16 kg of perilla leaves, grind the six medicinal materials, add 6 times the weight of the total medicinal materials in water, soak for 2 hours, and then extract the effective ingredients in a traditional Chinese medicine extraction tank for 7-9 hours, filter, and obtain mulberry and ginger water extract;

[0093] (2) The mulberry ginger water extract obtained in step (1) is concentrated to a relative density of 1.2-1.5, ethanol is added to make the ethanol concentration in the liquid to be 70%, and after standing for 8-12 hours, high-speed centrifugation is performed to obtain the supernatant.

[0094] (3) Concentrating the supernatant obtained in step (2), recovering ethanol, and obtaining mulberry and ginger extract.

[0095] (4) The remaining mulberry leaves are crushed and sieved, and evenly mixed with the mulberry and ginger extract obtained in step (3) to prepare granules.

[0096] (5) According to production requirements, the mulberry and ginger particles obtained in step (4) are made into tablets, capsules, granules, etc.

[0097] Reference Example 2

[0098] (1) According to the mulberry and ginger formula ratio, 30 kg of mulberry leaves, 16 kg of forsythia, 12 kg of chrysanthemum, 10 kg of dried ginger, 16 kg of bitter almonds, and 16 kg of perilla leaves were weighed, and the six medicinal materials were crushed, and water in an amount of 4 times the total weight of the medicinal materials was added, and the mixture was soaked for 3 hours, and then the effective ingredients were extracted in a traditional Chinese medicine extraction tank for 6 hours, and filtered to obtain a mulberry and ginger water extract;

[0099] (2) The mulberry ginger water extract obtained in step (1) is concentrated to a relative density of 1.2-1.5, 95% ethanol is added to the liquid to obtain an ethanol concentration of 80%, and after standing for 8-12 hours, high-speed centrifugation is performed to obtain the supernatant.

[0100] (3) Concentrating the supernatant obtained in step (2), recovering ethanol, and obtaining mulberry and ginger extract.

[0101] (4) According to production needs, the mulberry ginger extract obtained in step (3) is used with appropriate auxiliary materials and then subjected to corresponding process to prepare mulberry ginger tablets, mulberry ginger granules, mulberry ginger capsules, mulberry ginger oral liquid and other preparations.

[0102] Experimental Example 1 Screening of the Natural Clarifying Agent of the Present Invention

[0103] 1. Instruments and test drugs:

[0104] Ultraviolet spectrophotometer; electronic balance (1 / 10,000); high-speed centrifuge; ultra-high pressure extractor; chitosan; juice 101; ZTC1+1Ⅱ.

[0105] 2. Methods and Results

[0106] 2.1 Preparation of mulberry and ginger extract

[0107] Weigh 30g of mulberry leaves, 16g of forsythia, 12g of chrysanthemum, 10g of dried ginger, 16g of bitter almonds, and 16g of perilla leaves.

[0108] (1) After the weighed mulberry leaves, forsythia suspensa and chrysanthemum are crushed together, they are soaked in water 3 times the total weight of the three herbs for 2 hours. The wet herbs and their soaking liquid are subjected to ultrahigh pressure extraction at 400 MPa for 3 times. The three extracts are combined to obtain extract I.

[0109] (2) The weighed dried ginger, bitter almonds and perilla leaves are subjected to corresponding ultra-high extraction in the same manner as in step (1) to obtain extract II.

[0110] (3) combining the two extracts obtained in step (1) and step (2) to obtain a mulberry and ginger extract.

[0111] 2.2 Preparation of clarifier

[0112] (1) Preparation of chitosan solution: Weigh an appropriate amount of chitosan powder and use 1% acetic acid solution to prepare a 1% acetic acid solution containing 1% chitosan.

[0113] (2) Preparation of Juice 101 Solution: Weigh an appropriate amount of Juice 101 powder and use 1% acetic acid solution to prepare a 1% acetic acid solution containing 1% Juice 101.

[0114] (3) Preparation of ZTC1+1Ⅱ: Weigh an appropriate amount of component A in ZTC1+1Ⅱ and prepare a 1% component A solution with 1% acetic acid, that is, first dissolve it with a small amount of 1% acetic acid, stir it thoroughly, and then add the calculated amount of 1% acetic acid solution to swell for 24 hours after the viscose solution is formed. Use 1% acetic acid to prepare a 1% component B solution in the same way.

[0115] 2.3 Clarification process

[0116] (1) Take three portions of 50 ml of the above mulberry and ginger extract and number them as I, II, and III.

[0117] (2) Add 10% of the prepared chitosan solution to the mulberry and ginger extract No. I; add 10% of the fruit juice 101 solution to No. II; first add 10% of the B component solution to No. III, stir, and then add 5% of the A component solution.

[0118] (3) The temperature of the three groups of samples was maintained at 50°C. After standing for 6 hours, the floccules were observed and centrifuged at high speed to obtain the supernatant of the three groups of samples.

[0119] (4) Use an ultraviolet spectrophotometer to measure the transmittance of the three groups of supernatants and record the results.

[0120] 2.4 Experimental results are shown in Table 1

[0121] No. I No. II No. III Floc situation Small granular, filamentous Filamentous Filamentous, flocculent Light transmittance 67％ 50％ 73％

[0122] The results in the table show that under the same conditions, the natural clarifier ZTC1+1Ⅱ is the best choice for removing impurities from mulberry and ginger extract.

Claims

1. A method for preparing mulberry and ginger extract, Features: The traditional Chinese medicine formula for preparing the mulberry ginger extract is 30 parts of mulberry leaves, 16 parts of forsythia, 12 parts of chrysanthemum, 10 parts of dried ginger, 16 parts of bitter almonds, and 16 parts of perilla leaves. Ultrahigh pressure extraction is adopted, and then a clarifier is adopted for purification.

2. According to the method for preparing a mulberry and ginger extract as described in claim 1, Features: The following steps are involved: (1) taking mulberry leaves, forsythia suspensa, and chrysanthemum in the proportion of mulberry and ginger as combination I, and taking dried ginger, bitter almonds, and perilla leaves in the proportion of mulberry and ginger as combination II; (2) respectively crushing the combination I and II, adding 4-5 times the weight of the medicinal materials to the crushed I and II, respectively, and soaking for 2-5 hours; (3) the wet medicinal material obtained in step (2) and its soaking solution are placed in special containers, respectively, and ultrahigh pressure extraction is performed on each container, and finally the two extracts are combined; (4) adding a clarifier to the combined extract obtained in step (3), stirring evenly, maintaining the temperature at 35-65° C., standing for 4-5 hours, centrifuging at high speed, and taking the supernatant; (5) performing microfiltration on the supernatant obtained in step (4) to obtain a mulberry ginger filtrate; (6) concentrating the mulberry and ginger filtrate obtained in step (5) to obtain a mulberry and ginger extract.

3. According to the preparation method described in claim 1, 2, Features: The medium to ultrahigh pressure extraction has a pressure of 400-900 MPa, preferably 500 MPa.

4. According to the preparation method described in claim 3, Features: The ultra-high pressure extraction is performed 3 to 6 times.

5. According to the preparation method described in claim 3, Features: The ultra-high pressure extraction has an extraction time of 18-30 minutes.

6. According to the preparation method described in claim 1, 2, Features: The clarifier is one or more of ethanol chitosan, fruit juice 101 and / or ZTC1+1Ⅱ.

7. The preparation method according to claim 6, Features: The clarifier in step (4) is ZTC1+1Ⅱ, and the amount of components A and B added is 1:2 based on the volume of the liquid.

8. According to the preparation method described in claim 2, Features: The standing time in step (4) is 3-8 hours, preferably 6-8 hours.

9. According to the preparation method described in claim 2, Features: The pore size of the microporous filtration in step (5) is 1.2 μm.

10. The preparation method according to claims 1-9, Features: The mulberry ginger extract can be prepared into mulberry ginger solid preparations (tablets, capsules, granules, pills, etc.) or mulberry ginger liquid preparations through corresponding process according to production needs by adding auxiliary materials in appropriate proportions.