Device and method for extracting platelet-rich plasma

Abstract

The invention discloses a platelet-rich plasma extraction device and method, the platelet-rich plasma extraction device comprises a base, a rotating disc, an injector, a support mechanism and a motor, the support mechanism comprises a supporting plate, a sleeve, a connecting rod and a rotating wheel, the supporting plate is relatively fixedly installed on the rotating disc, the rotating wheel penetrates through the supporting plate to be fixedly connected with the sleeve, and the connecting rod is fixedly connected with the rotating wheel. The sleeve is fixedly connected with the connecting rod, the connecting rod is rotationally connected with the supporting plate on the other side, a first groove is formed in the bottom of the sleeve and connected with the syringe needle base in an inserted mode, and a through hole corresponding to the syringe needle base is formed in the bottom of the sleeve. And a needle head connecting part is arranged at the through hole at the bottom of the sleeve. The blood taking device has the beneficial effects that the syringe is fixed in the sleeve, and the blood taking needle is fixed on the sleeve in a sticking manner during centrifugal operation, so that the contact between blood and the outside is avoided, and the risk of pollution in the preparation process of platelet-rich plasma is reduced.

Description

Technical Field

[0001] The present invention relates to the technical field of medical devices, and in particular to a device and method for extracting platelet-rich plasma. Background Art

[0002] Platelet-rich plasma (PRP) is a platelet concentrate obtained by centrifuging whole blood. In addition to providing coagulation during hemostasis, it can also induce the division and proliferation of bone cells and collagen synthesis, promote the repair and reconstruction of bone tissue, and is widely used in orthopedics, dentistry, sports medicine and other fields. The principle of PRP preparation is to use the different sedimentation rates of various components in the blood to separate the blood into layers by centrifugation. Whole blood can be divided into three layers by low-speed centrifugation. The upper layer is the platelet-poor plasma layer (PPP), the middle layer is the platelet-rich plasma layer (PRP), and the lower layer is the red blood cell layer. Taking the middle layer can obtain highly concentrated platelet-containing plasma.

[0003] There are PRP machines for collection at home and abroad, but they are expensive and time-consuming to operate. The basic principle requires two centrifuge tubes, two extractions of plasma, which are easy to be contaminated, and there are different degrees of loss when platelets are extracted. The traditional two-centrifugation method requires two centrifuge tubes, namely a centrifuge tube containing sodium citrate anticoagulant and a centrifuge tube without anticoagulant; a blood collection syringe or blood collection needle; two long needles: the first one is used to extract the 2-3mm red blood cell layer and plasma layer below the white film layer after the first centrifugation, and the second one is used to extract the upper 3 / 4 plasma layer after the second centrifugation; two syringes for extracting PRP; 10% calcium chloride solution; human thrombin lyophilized powder; low-temperature high-speed centrifuge; sterile environment such as clean bench. Its disadvantages are high contamination rate, incomplete white film layer, low accuracy, many operation steps, and time-consuming. Summary of the invention

[0004] In view of the above-mentioned prior art, the present invention provides a device and method for extracting platelet-rich plasma, which mainly solves the technical problems existing in the above-mentioned background technology.

[0005] To achieve the above object, the technical solution of the embodiment of the present invention is implemented as follows:

[0006] A device for extracting platelet-rich plasma comprises a base, a turntable, a syringe, a support mechanism and a motor, wherein the turntable is arranged in the middle of the base, the motor is installed in the base, the motor is fixedly connected to the turntable, the support mechanism comprises a support plate, a sleeve, a connecting rod and a rotating wheel, the support plate is relatively fixedly installed on the turntable, the rotating wheel passes through the support plate and is fixedly connected to the sleeve, the sleeve is fixedly connected to the connecting rod, the connecting rod is rotatably connected to the support plate on the other side, a first groove is arranged at the bottom of the sleeve, the first groove is plugged into the syringe needle seat, a through hole is arranged at the bottom of the sleeve corresponding to the syringe needle seat, a needle connecting part is arranged at the bottom of the sleeve at the through hole, the syringe piston shaft is detachably connected to the piston in the syringe, and the top end of the sleeve is detachably connected to the sealing cover.

[0007] Preferably, the piston shaft cooperates with a threaded hole provided at the bottom of the piston and is fixed by screwing.

[0008] Preferably, the length of the syringe is the same as the inner length of the sleeve.

[0009] Preferably, the side wall of the rotating wheel is provided with a second groove, a spring is fixedly installed at the bottom of the second groove, the other end of the spring is fixedly connected to the spring block, and the outer side wall of the support plate is provided with a slot corresponding to the spring block.

[0010] Preferably, the arc diameter of the outer side wall of the spring block is the same as the diameter of the rotating wheel.

[0011] Preferably, two of the card slots are provided along the axis of the support plate.

[0012] Preferably, a suction cup is provided at the bottom of the base.

[0013] The present invention also discloses a method for preparing leukocyte-reduced platelet-rich plasma, comprising the following steps:

[0014] S1: Open the sealing cover, put the syringe into the sleeve and plug it into the first groove to fix it, connect the needle connection part at the bottom of the sleeve to the blood collection needle, pull the piston shaft to extract the anticoagulant and blood, pull out the needle of the blood collection needle and seal it with a rubber cap, stick the blood collection needle to the sleeve, remove the piston shaft of the syringe, and cover the sealing cover;

[0015] S2: Turn the rotating wheel to make the syringe needle seat face down and fix it, start the motor, the rotating disk speed is 3000rpm, the rotating time is 3-6 minutes, after the rotation is completed, the blood is stratified, the lower layer is the red blood cell RBC layer, the middle layer is the white blood cell WBC and platelet PLE buffy coat layer, and the upper layer is the plasma layer;

[0016] S3: Tear off the blood collection needle and take off the rubber cap, open the sealing cover, connect the piston shaft of the syringe with the piston, push the piston shaft of the syringe to discharge the red blood cell layer close to the syringe outlet from the needle, retain the white film layer and the plasma layer, rotate the rotating wheel, remix the blood in the syringe, and make the syringe needle seat face up and fix it, seal the needle with a rubber cap, stick the blood collection needle on the cannula, remove the piston shaft of the syringe, and cover the sealing cover;

[0017] S4: Start the motor, the rotating disk rotates at 3000 rpm, and rotates for 10-15 minutes. After the rotation is completed, the blood is separated into layers, the upper layer is the plasma layer, and the lower layer is the buffy coat layer including white blood cells (WBC) and platelets (PLE);

[0018] S5: Tear off the blood collection needle and take off the rubber cap, open the sealing cover, connect the piston shaft of the syringe with the piston, push the piston shaft of the syringe to discharge the plasma layer near the syringe outlet from the needle, and the remaining white film layer is platelet-rich plasma.

[0019] The beneficial effects of the present invention are:

[0020] (1) By fixing the syringe in the cannula and fixing the blood collection needle on the cannula by gluing during the centrifugal operation, the contact between blood and the outside world is avoided, thereby reducing the risk of contamination during the preparation of platelet-rich plasma.

[0021] (2) The direction of the syringe needle seat is fixed by the rotating wheel, which facilitates the removal of other components in the blood after centrifugation and retains the platelet-rich plasma, thereby improving work efficiency. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 This is a schematic cross-sectional structure diagram of a platelet-rich plasma extraction device during centrifugation in an embodiment of the present application;

[0023] Figure 2 This is a schematic diagram of the cross-sectional structure of a platelet-rich plasma extraction device in an embodiment of the present application when drawing blood;

[0024] Figure 3 for Figure 2 The enlarged schematic diagram of part A in the middle;

[0025] Figure 4 This is a schematic diagram of an inverted structure of a platelet-rich plasma extraction device in an embodiment of the present application;

[0026] Figure 5 This is a front view schematic diagram of the rotating wheel in the embodiment of the present application;

[0027] Description of Figure Numbers:

[0028] 1. Base; 2. Turntable; 3. Syringe; 4. Bracket mechanism; 401. Support plate; 4011. Card slot; 402. Sleeve; 403. Connecting rod; 404. Rotating wheel; 4041. Second groove; 4042. Spring; 4043. Spring block; 5. Motor; 6. First groove; 7. Through hole; 8. Needle connecting part; 9. Sealing cover; 10. Threaded hole; 11. Suction cup. DETAILED DESCRIPTION

[0029] The technical solution of the present invention is further elaborated in detail below in conjunction with the drawings and specific embodiments of the specification. Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by technicians in the technical field of the present invention. The terms used in the specification of the present invention herein are only for the purpose of describing specific embodiments and are not intended to limit the present invention. In the following description, the expression "some embodiments" is related to a subset of all possible embodiments, but it should be understood that "some embodiments" can be the same subset or different subsets of all possible embodiments, and can be combined with each other without conflict.

[0030] It should also be noted that when an element is referred to as being "fixed to" another element, it may be directly on the other element or there may be a central element. When an element is considered to be "connected to" another element, it may be directly connected to the other element or there may be a central element at the same time. The terms "vertical", "horizontal", "inside", "outside", "left", "right" and similar expressions used herein are for illustrative purposes only and are not intended to be the only implementation method.

[0031] Example 1

[0032] Please refer to the attached Figure 1-4The present application provides a platelet-rich plasma extraction device, comprising a base 1, a turntable 2, a syringe 3, a support mechanism 4 and a motor 5. The turntable 2 is arranged in the middle of the base 1, the motor 5 is installed in the base 1, the motor 5 is fixedly connected to the turntable 2, the support mechanism 4 comprises a support plate 401, a sleeve 402, a connecting rod 403 and a rotating wheel 404, the support plate 401 is relatively fixedly installed on the turntable 2, the rotating wheel 404 passes through the support plate 401 and is fixedly connected to the sleeve 402, the The sleeve 402 is fixedly connected to the connecting rod 403, and the connecting rod 403 is rotatably connected to the support plate 401 on the other side. A first groove 6 is provided at the bottom of the sleeve 402, and the first groove 6 is plugged into the needle seat of the syringe 3. A through hole 7 corresponding to the needle seat of the syringe 3 is provided at the bottom of the sleeve 402. A needle connecting part 8 is provided at the bottom of the sleeve 402 at the through hole 7. The piston shaft of the syringe 3 is detachably connected to the piston inside the syringe 3, and the top of the sleeve 402 is detachably connected to the sealing cover 9.

[0033] Open the sealing cover 9 on the sleeve 402, put the syringe 3 into the sleeve 402 and plug it in to fix it, so that the syringe 3 and the sleeve 402 form a whole, which is convenient for subsequent centrifugation operation. The needle connecting part 8 at the bottom of the sleeve 402 is connected to the blood collection needle, and the anticoagulant and blood are extracted through the blood collection needle and the syringe 3. The rotating wheel 404 is rotated to mix the blood and anticoagulant in the syringe 3. After mixing, the rotating wheel 404 can be rotated to put the needle seat of the syringe 3 upright or inverted, which is convenient for subsequent secondary centrifugation operation, while removing other components in the blood, retaining platelet-rich plasma, and improving work efficiency. After fixing the rotating wheel 404, remove the piston shaft of the syringe 3, cover it with the sealing cover 9 for sealing. The function of the sealing cover 9 is to limit the syringe 3 to prevent the syringe 3 from detaching from the sleeve 40 during centrifugation. 2, causing centrifugal failure, pulling out the needle of the blood collection needle and sealing it with a rubber cap, sticking the blood collection needle on the sleeve 402, starting the motor 5, driving the turntable 2 to rotate, and at the same time, the syringe 3 in the bracket mechanism 4 rotates coaxially with the turntable 2, and the blood mixture in the syringe 3 is centrifuged. After the centrifugation is completed, open the sealing cover 9, connect the piston shaft of the syringe 3 with the piston, push the piston shaft of the syringe 3, and discharge the non-platelet-rich plasma components after the blood separation at the outlet of the syringe 3, and finally obtain high-purity platelet-rich plasma. By fixing the syringe 3 in the sleeve 402 and fixing the blood collection needle on the sleeve 402 by gluing during the centrifugal operation, the contact between the blood and the outside world is avoided, and the risk of contamination during the preparation of platelet-rich plasma is reduced. Preferably, the support plate 401 is provided with a groove for storing the rotating part of the rotating wheel 404, and the depth of the groove is 1 / 3 of the thickness of the rotating part of the rotating wheel 404. The contact surface between the groove of the support plate 401 and the rotating part of the rotating wheel 404 are both made of rough material. After the rotating wheel 404 is fixed, the rotating wheel 404 is locked by friction.

[0034] Furthermore, the piston shaft is matched with the threaded hole 10 provided at the bottom of the piston and is fixed by screwing. The piston shaft is screwed and fixed with the threaded hole 10 provided at the bottom of the piston, which is convenient for installation and disassembly.

[0035] Furthermore, the length of the syringe 3 is the same as the inner length of the sleeve. The sealing cover 9 can limit the position of the syringe 3 while sealing, so as to prevent the needle seat of the syringe 3 from being separated from the sleeve 402 by the centrifugal force during centrifugation, thereby affecting the centrifugal effect.

[0036] Example 2

[0037] Please refer to the attached Figure 1-5The difference between this embodiment and the first embodiment is that the side wall of the rotating wheel 404 is provided with a second groove 4041, a spring 4042 is fixedly installed at the bottom of the second groove 4041, the other end of the spring 4042 is fixedly connected to the spring block 4043, and the outer wall of the support plate 401 is provided with a slot 4011 corresponding to the spring block 4043. The spring 4042 is fixedly installed in the second groove 4041 of the rotating wheel 404, and the spring 4042 is fixedly connected to the spring block 4043. Under the elastic force of the spring 4042, the spring block 4043 is engaged with the slot 4011 on the support plate 401, which plays a role in fixing the direction of the syringe 3, and is convenient for determining the direction of the needle seat of the syringe 3 during centrifugation. Preferably, when the spring block 4043 is engaged with the slot 4011, the needle seat of the syringe 3 is upright or inverted.

[0038] Further, the outer wall of the spring block 4043 has the same diameter as the rotating wheel 404. The outer wall of the spring block 4043 is arranged in an arc shape, and the diameter of the arc shape is the same as the diameter of the rotating wheel 404, so that the spring block 4043 can be rotated in the groove on the support plate 401 after being compressed into the rotating wheel 404. Preferably, the outer wall of the slot 4011 is also arranged in an arc shape, and the diameter of the arc shape is the same as the diameter of the rotating wheel 404.

[0039] Furthermore, two clamping slots 4011 are provided along the axis of the support plate 401. Two vertical clamping slots 4011 are provided, one above the other, to facilitate fixing the direction of the syringe 3.

[0040] Furthermore, a suction cup 11 is provided at the bottom of the base 1. The base 1 is fixed on a plane by the suction cup 11 at the bottom of the base 1, thereby improving the stability of the device.

[0041] Using the above device, the present invention also discloses a method for preparing leukocyte-reduced platelet-rich plasma, comprising the following steps:

[0042] S1: fix the base on a plane, open the sealing cover 9, put the syringe 3 into the sleeve 402 and plug it into the first groove 6, connect the needle connection part 8 at the bottom of the sleeve 402 to the blood collection needle, pull the piston shaft to extract the anticoagulant and blood, pull out the needle of the blood collection needle and seal it with a rubber cap, stick the blood collection needle to the sleeve 402, remove the piston shaft of the syringe 3, and cover the sealing cover 9;

[0043] S2: Rotate the rotating wheel 404 to make the needle seat of the syringe 3 face downward and fix it, start the motor 5, rotate the turntable 2 at a speed of 3000 rpm, and rotate for 3-6 minutes. After the rotation is completed, the blood is stratified, with the lower layer being the red blood cell RBC layer, the middle layer being the buffy coat layer including white blood cells WBC and platelets PLE, and the upper layer being the plasma layer;

[0044] S3: tear off the blood collection needle and take off the rubber cap, open the sealing cover 9, connect the piston shaft of the syringe 3 with the piston, push the piston shaft of the syringe 3, make the red blood cell layer near the outlet of the syringe 3 be discharged from the needle, keep the white film layer and the plasma layer, rotate the rotating wheel 404, remix the blood in the syringe 3, and make the needle seat of the syringe 3 face up and fix it, seal the needle with the rubber cap, stick the blood collection needle on the sleeve 402, remove the piston shaft of the syringe 3, and cover the sealing cover 9;

[0045] S4: start the motor 5, the rotating disk 2 rotates at a speed of 3000 rpm, and the rotating time is 10-15 minutes. After the rotating is completed, the blood is stratified, the upper layer is the plasma layer, and the lower layer is the buffy coat layer including white blood cells WBC and platelets PLE;

[0046] S5: Tear off the blood collection needle and the rubber cap, open the sealing cover 9, connect the piston shaft of the syringe 3 with the piston, push the piston shaft of the syringe 3, so that the plasma layer near the outlet of the syringe 3 is discharged from the needle, and the remaining white film layer is platelet-rich plasma.

[0047] It should be noted that the platelet-rich plasma finally obtained, after being shaken evenly, can be connected to the injection needle through the needle connection part 8 for injection.

[0048] The above are only specific embodiments of the present invention, but the protection scope of the present invention is not limited thereto. Any person skilled in the art can easily think of changes or substitutions within the technical scope disclosed by the present invention, which should be included in the protection scope of the present invention. The protection scope of the present invention should be based on the protection scope of the claims.

Claims

1. A platelet-rich plasma extraction device, characterized in that: It includes a base, a turntable, a syringe, a bracket mechanism and a motor. The turntable is arranged in the middle of the base, the motor is installed in the base, and the motor is fixedly connected to the turntable. The bracket mechanism includes a support plate, a sleeve, a connecting rod and a rotating wheel. The support plate is relatively fixedly installed on the turntable, the rotating wheel passes through the support plate and is fixedly connected to the sleeve, the sleeve is fixedly connected to the connecting rod, and the connecting rod is rotatably connected to the support plate on the other side. A first groove is arranged at the bottom of the sleeve, and the first groove is plugged into the syringe needle seat. A through hole corresponding to the syringe needle seat is arranged at the bottom of the sleeve, and a needle connecting part is arranged at the bottom of the sleeve at the through hole. The syringe piston shaft is detachably connected to the piston in the syringe, and the top end of the sleeve is detachably connected to the sealing cover.

2. A platelet-rich plasma extraction device according to claim 1, characterized in that: The piston shaft matches with the threaded hole arranged at the bottom of the piston and is fixed by screwing.

3. The platelet-rich plasma extraction device according to claim 1, characterized in that: The length of the syringe is the same as the inner length of the sleeve.

4. The platelet-rich plasma extraction device according to claim 1, characterized in that: The side wall of the rotating wheel is provided with a second groove, a spring is fixedly installed at the bottom of the second groove, the other end of the spring is fixedly connected to the spring block, and the outer side wall of the support plate is provided with a slot corresponding to the spring block.

5. The platelet-rich plasma extraction device according to claim 4, characterized in that: The arc diameter of the outer side wall of the spring block is the same as the diameter of the rotating wheel.

6. The platelet-rich plasma extraction device according to claim 4, characterized in that: The clamping slots are provided with two along the axis of the support plate.

7. The platelet-rich plasma extraction device according to claim 1, characterized in that: A suction cup is provided at the bottom of the base.

8. A method for extracting platelet-rich plasma, characterized in that: A platelet-rich plasma extraction device according to any one of claims 1 to 7 is implemented, comprising the following steps: S1: Open the sealing cover, put the syringe into the sleeve and plug it into the first groove to fix it, connect the needle connection part at the bottom of the sleeve to the blood collection needle, pull the piston shaft to extract the anticoagulant and blood, pull out the needle of the blood collection needle and seal it with a rubber cap, stick the blood collection needle to the sleeve, remove the piston shaft of the syringe, and cover the sealing cover; S2: Turn the rotating wheel to make the syringe needle seat face down and fix it, start the motor, the rotating disk speed is 3000rpm, the rotating time is 3-6 minutes, after the rotation is completed, the blood is stratified, the lower layer is the red blood cell RBC layer, the middle layer is the white blood cell WBC and platelet PLE buffy coat layer, and the upper layer is the plasma layer; S3: Tear off the blood collection needle and take off the rubber cap, open the sealing cover, connect the piston shaft of the syringe with the piston, push the piston shaft of the syringe to discharge the red blood cell layer close to the syringe outlet from the needle, retain the white film layer and the plasma layer, rotate the rotating wheel, remix the blood in the syringe, and make the syringe needle seat face up and fix it, seal the needle with a rubber cap, stick the blood collection needle on the cannula, remove the piston shaft of the syringe, and cover the sealing cover; S4: Start the motor, the rotating disk rotates at 3000 rpm, and rotates for 10-15 minutes. After the rotation is completed, the blood is separated into layers, the upper layer is the plasma layer, and the lower layer is the buffy coat layer including white blood cells (WBC) and platelets (PLE); S5: Tear off the blood collection needle and take off the rubber cap, open the sealing cover, connect the piston shaft of the syringe with the piston, push the piston shaft of the syringe to discharge the plasma layer near the syringe outlet from the needle, and the remaining white film layer is platelet-rich plasma.

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