Porous hydrophilic biological wax slow-release carbon source as well as preparation method and application thereof

Abstract

The invention relates to the technical field of water treatment, in particular to a porous hydrophilic biological wax slow-release carbon source and a preparation method and application thereof. The porous hydrophilic biological wax slow-release carbon source is prepared from the following raw materials in parts by mass: 20-40 parts of beewax, 20-40 parts of lignite wax and 20-80 parts of a pore-forming agent solution. The porous hydrophilic biological wax slow-release carbon source and the preparation method thereof provided by the invention have remarkable technical advantages in the aspects of increasing the adhesion amount of microorganisms, enhancing the adsorption capacity of pollutants, realizing uniform distribution of pores, continuously releasing nutrients and the like, and have an important application value in eutrophication treatment of small and medium-sized water bodies.

Description

Technical Field

[0001] The present application relates to the field of water treatment technology, and in particular to a porous hydrophilic biowax slow-release carbon source and a preparation method and application thereof. Background Art

[0002] The cause of eutrophication of the water environment is usually related to the increase in the content of nutrients such as nitrogen and phosphorus. When excessive nutrients enter the water, aquatic organisms and microorganisms represented by algae will obtain sufficient reproduction conditions, thereby multiplying and accumulating in large numbers, forming a layer of "microbial film" on the surface of the water, reducing the light transmittance of the water, resulting in a decrease in the dissolved oxygen content of the water, causing the death of aquatic organisms such as fish, and the water will change color and stink, losing its value.

[0003] Most of them are static or poorly mobile water bodies. Due to their small water area, they are easily polluted and have poor self-purification ability. In addition, due to different degrees of pollution and water quality management problems, they have gradually lost their original functions and seriously affected the natural environment of the city and people's living environment. Physical methods, chemical methods and biological methods such as adding microbial agents and aquatic plants are commonly used in the market to control eutrophication problems.

[0004] Microorganisms are one of the decomposers in the natural water system. They transform and degrade pollutants through chemical reactions catalyzed by enzymes. Biological methods have the advantages of low cost and sustainability, but they require a long cycle, so a carbon source needs to be provided.

[0005] In the prior art, water eutrophication treatment mostly adopts physical methods, chemical methods or direct addition of microbial agents, but there are problems such as high cost, easy to cause secondary pollution, and long cycle. Although traditional carbon sources such as powdered activated carbon or sodium acetate can promote microbial growth, they have poor solubility and poor sustained release effect. In addition, the pore structure of existing sustained release materials is uneven, resulting in low microbial attachment and unstable denitrification and phosphorus removal efficiency. Summary of the invention

[0006] In order to solve the problem of repeated eutrophication in small and medium-sized water bodies due to the increase in the concentration of ammonia nitrogen and total phosphorus in the water, the present application provides a porous hydrophilic biowax slow-release carbon source and its preparation method and application, which uses lignite wax as a high-efficiency material for river and lake management, provides nutrients, trace elements and breeding sites for the growth and reproduction of beneficial microorganisms in the water body, activates the activity of microorganisms in the water, accelerates the growth and reproduction of microorganisms in the water body, and metabolism, thereby improving the self-purification ability of the water body and achieving the purpose of improving the water body. By optimizing the ratio of beeswax, lignite wax and pore-forming agent, and combining the aeration and stirring process, a uniform microporous structure is formed, which significantly improves the amount of microbial attachment and the efficiency of nitrogen and phosphorus removal, and is suitable for the eutrophication control of small and medium-sized water bodies.

[0007] In the first aspect, the present application provides a porous hydrophilic biowax slow-release carbon source, using the following technical solution: A porous hydrophilic biowax slow-release carbon source comprises the following raw materials by weight: 20-40 parts of beeswax, 20-40 parts of montan wax and 20-80 parts of pore-forming agent solution.

[0008] By adopting the above technical scheme, beeswax and lignite wax: these two waxes are used as the main raw materials to provide the matrix and skeleton structure of the material. Beeswax has good hydrophilicity and biodegradability, while lignite wax provides a certain carbon source and stability. The combination of these two waxes can form a stable biowax matrix, while providing nutrients and trace elements required for the growth and reproduction of microorganisms. Pore-forming agent solution: The pore-forming agent forms a microporous structure in the material. These micropores not only increase the specific surface area of ​​the material, provide more microbial attachment points, but also help to improve the hydrophilicity and biodegradability of the material. At the same time, the microporous structure also helps to improve the sustained release performance of the material, so that the carbon source can be continuously and effectively released to the microorganisms in the water body. For example, polyvinyl pyrrolidone (PVP) is selected as a pore-forming agent. PVP plays a role in adjusting the pore structure and controlling the pore size during the material preparation process. By optimizing the dosage and ratio of PVP, a biowax material with a uniform microporous structure can be prepared, thereby increasing the attachment amount and biological activity of microorganisms. In summary, beeswax and lignite wax provide the matrix and nutrient source of biowax materials, and pore-forming agents improve the hydrophilicity and biological activity of materials by forming microporous structures, thereby enhancing the self-purification ability of water bodies and improving water quality.

[0009] Preferably, the mass ratio of the beeswax, montan wax and pore-forming agent solution is 1:2:2.

[0010] By adopting the above technical scheme, beeswax has good hydrophilicity and biodegradability, and can be used as the main material of the bio-wax slow-release carbon source. The addition of beeswax helps to improve the stability and durability of the material, and also increases the hydrophilicity of the material, making it easier to disperse in water and be used by microorganisms. Montan wax is a natural organic matter rich in carbon elements and other organic substances, which can be provided as a carbon source to microorganisms in water bodies. The addition of montan wax helps to increase the carbon content and biological activity of the material, and also enhances the slow-release performance of the material, so that the carbon source can be released continuously and stably. The function of the pore-forming agent solution is to form a microporous structure during the preparation of the material. These micropores can not only increase the specific surface area of ​​the material and increase the amount of microbial attachment, but also promote material exchange and microbial metabolic activities inside the material. At the same time, the microporous structure helps to improve the slow-release performance and biological activity of the material. In summary, when the mass ratio of beeswax, montan wax and pore-forming agent solution is 1:2:2, it can effectively combine their respective advantages to form an efficient porous hydrophilic bio-wax slow-release carbon source. This material can provide a suitable growth environment for microorganisms in water bodies, activate the activity of microorganisms, accelerate the metabolism of microorganisms, thereby improving the self-purification ability of water bodies and effectively improving the eutrophication problem of water bodies.

[0011] Preferably, the pore-forming agent solution is a polyvinyl pyrrolidone aqueous solution with a mass concentration of 90-110 g / L or a polyethylene glycol aqueous solution with a mass concentration of 60-70 g / L.

[0012] Preferably, the pore diameter of the bio-wax slow-release carbon source is 0.05-0.10 mm.

[0013] Preferably, it also includes 4-8 parts of activated carbon with a particle size of 200-325 meshes.

[0014] In a second aspect, the present application provides a method for preparing a porous hydrophilic biowax slow-release carbon source, using the following technical solution: As a general technical concept, the present application also provides a method for preparing the above-mentioned porous hydrophilic bio-wax slow-release carbon source, comprising the following steps: S61, adding beeswax with a particle size of 0.5-1 cm and montan wax with a particle size of 0.5-1 cm into a reactor respectively according to their mass fractions, heating in a water bath to completely melt the beeswax and montan wax into a liquid state, and obtaining liquid A; S62, adding the pore-forming agent solution to the liquid A according to the mass fractions and mixing evenly to obtain a mixed solution; S63, pouring the mixed solution into a heat-resistant mold and cooling it to form a porous hydrophilic bio-wax slow-release carbon source.

[0015] By adopting the above technical solution, S61 provides a carbon source and a structural basis, S62 introduces a pore former to form pores, and S63 is shaped and forms a porous structure. The synergistic effect lies in the fact that the structure and composition of the material jointly promote the attachment, growth and metabolism of microorganisms, thereby effectively treating eutrophic water bodies. S61: Melting of beeswax and lignite wax, beeswax and lignite wax as the main carriers, provide carbon sources, mineral elements and slow-release matrix. Beeswax (beeswax) is a natural hydrophobic substance that can form a slow-release skeleton; lignite wax contains humic acid, trace elements and mineral components, which can supplement the nutrients required for microbial metabolism. It is melted into a liquid by heating in a water bath to ensure that the two waxes are evenly mixed to form a homogeneous matrix, providing a basis for subsequent pore formation. S62: Mixing of pore former solutions, water-soluble pore formers, and introducing pore structures through physical mixing. After the pore former is dispersed in the wax liquid, it dissolves in contact with water during cooling to form microporous channels. The pore structure increases the specific surface area, provides attachment sites for microorganisms, and enhances hydrophilicity to promote the diffusion of nutrients in the water body. S63: Cooling and molding, cooling and shaping through the mold to form a stable porous hydrophilic structure, and the wax solidifies during the cooling process.

[0016] Preferably, in S62, the process further includes adding the pore-forming agent solution and activated carbon into the liquid A and mixing them evenly to obtain a mixed liquid.

[0017] Preferably, in step S61, the water bath is heated to a temperature of 80-90°C.

[0018] Preferably, in step S62, the uniform mixing method is stirring for 4-8 minutes at a mechanical stirring speed of 600 r / min or using aeration treatment to increase the amount of internal bubbles, the aeration rate is 250 L / min, and the aeration time is 4-8 minutes.

[0019] By adopting the above technical solutions, 1. The core role of mechanical stirring (600r / min): to achieve uniform dispersion of pore-forming agent (polyvinyl pyrrolidone) and molten wax matrix, avoid local agglomeration, and ensure the uniformity of subsequent pore structure. 2. The unique contribution of aeration treatment (250L / min): the physical disturbance of bubbles forms a three-dimensional mesh channel, forming a "micropore-mesopore-through hole" multi-level structure, and improving pore connectivity. The through-porosity directly affects the efficiency of microbial migration and material exchange. The bubble effect produces an open pore structure, which increases the specific surface area more than simple mechanical stirring. Aeration can also promote the directional arrangement of hydrophilic groups (pyrrolidone rings of polyvinyl pyrrolidone) and enhance the hydrophilicity of the material. The gas-liquid interface generated by aeration accelerates the phase separation during the solidification of the wax matrix, forming a nano-scale rough structure on the pore wall, and increasing the amount of microbial attachment.

[0020] In a third aspect, the present application provides an application of a porous hydrophilic biowax slow-release carbon source, using the following technical solution: As a general technical concept, the present application also provides the application of the above-mentioned porous hydrophilic bio-wax slow-release carbon source in the treatment of water eutrophication, and the porous hydrophilic bio-wax slow-release carbon source is suspended in the water body to promote the degradation of ammonia nitrogen and total phosphorus by microorganisms.

[0021] In summary, the present application includes at least one of the following beneficial technical effects: 1. Improve the amount of microbial attachment and the efficiency of nitrogen and phosphorus removal: By optimizing the ratio of beeswax, lignite wax and pore-forming agent (polyvinyl pyrrolidone), a uniform microporous structure is formed. This structure not only increases the attachment space of microorganisms, but also provides a good growth environment for microorganisms, thereby significantly improving the amount of microbial attachment and the efficiency of nitrogen and phosphorus removal.

[0022] 2. Enhance the adsorption capacity of pollutants: Combined with the use of activated carbon, the adsorption capacity of pollutants is further enhanced. Activated carbon has a large specific surface area and good adsorption performance, which can effectively adsorb organic matter and other pollutants in water, thereby improving the overall denitrification and phosphorus removal efficiency.

[0023] 3. Achieve uniform pore distribution: The aeration and stirring process ensures uniform pore distribution inside the biowax slow-release carbon source. This uniform distribution helps to increase the effective contact opportunities and reaction efficiency of microorganisms, and further improve the nitrogen and phosphorus removal effects.

[0024] 4. Continuous release of nutrients: Biowax slow-release carbon source can continuously release nutrients and other trace elements required by microorganisms in the water body, while maintaining the activity of microorganisms, avoiding frequent artificial carbon replenishment operations, reducing management costs and environmental burden.

[0025] 5. Suitable for small and medium-sized water treatment: Due to its uniform microporous structure and continuous nutrient release characteristics, the biowax slow-release carbon source of this application is particularly suitable for eutrophication treatment of small and medium-sized water bodies. It can effectively improve the self-purification capacity of these water bodies and reduce the occurrence of eutrophication problems. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the following briefly introduces the drawings used in the embodiments: Figure 1 This is a surface pore map of the bio-wax slow-release carbon source prepared in Comparative Example 1; Figure 2 This is a surface pore map of the bio-wax slow-release carbon source prepared in Comparative Example 2; Figure 3 This is a surface pore map of the bio-wax slow-release carbon source prepared in Example 3; Figure 4 This is an enlarged view of the surface pores of the bio-wax slow-release carbon source prepared in Example 3; Figure 5This is a surface pore map of the bio-wax slow-release carbon source prepared in Example 4; Figure 6 This is an enlarged view of the surface pores of the bio-wax slow-release carbon source prepared in Example 4; Figure 7 This is a surface pore map of the bio-wax slow-release carbon source prepared in Example 5; Figure 8 This is an enlarged view of the surface pores of the bio-wax slow-release carbon source prepared in Example 5; Fig. 9 A 7-day ammonia nitrogen removal rate curve of biowax slow-release carbon source biofilm was prepared for Example 3 and Example 4 and Comparative Examples 1 to Comparative Examples 3; Fig.10 A 14-day ammonia nitrogen removal rate curve of biowax slow-release carbon source biofilm formation was prepared for Example 3 and Example 4 and Comparative Examples 1 to Comparative Examples 3; Fig.11 A 21-day ammonia nitrogen removal rate curve of biowax slow-release carbon source biofilm was prepared for Example 3 and Example 4 and Comparative Examples 1 to Comparative Examples 3; Fig.12 The total phosphorus removal rate curve of the biowax slow-release carbon source biofilm formed in 7 days is prepared for Example 3 and Example 4 and Comparative Examples 1 to Comparative Examples 3; Fig.13 The total phosphorus removal rate curve of the biowax slow-release carbon source biofilm formed on 14 days was prepared for Example 3 and Example 4 and Comparative Examples 1 to Comparative Examples 3; Fig.14 The total phosphorus removal rate curve of 21 days of biowax slow-release carbon source biofilm formation was prepared for Example 3 and Example 4 and Comparative Examples 1 to Comparative Examples 3; Fig.15 This is the distribution map of the sampling points for Sanjian Lake detection. DETAILED DESCRIPTION

[0027] The embodiments of the present application will be described in detail below in conjunction with the examples, but it will be appreciated by those skilled in the art that the following examples are only used to illustrate the present application and should not be considered as limiting the scope of the present application. In the examples, if specific conditions are not specified, they are carried out according to normal conditions or the conditions recommended by the manufacturer. If the manufacturer is not specified for the reagents or instruments used, they are all conventional products that can be purchased commercially.

[0028] In the following examples, 1 portion means 100 g.

[0029] Example 1 A porous hydrophilic biowax slow-release carbon source, comprising the following raw materials by weight: 20 parts of beeswax, 20 parts of montan wax, and 20 parts of a pore-forming agent solution, wherein the pore-forming agent solution is a polyethylene glycol aqueous solution with a mass concentration of 65 g / L; The method for preparing the porous hydrophilic biowax slow-release carbon source adopts the following technical scheme: S61, adding beeswax with a particle size of 0.5-1 cm and montan wax with a particle size of 0.5-1 cm into a reactor respectively according to their mass fractions, heating the reactor in a water bath to 80° C., so that the beeswax and montan wax are completely melted into a liquid state, to obtain liquid A; S62, adding the pore-forming agent solution to liquid A according to the mass fraction, stirring for 5 minutes at a mechanical stirring speed of 600 r / min to obtain a mixed solution; S63, pouring the mixed solution into a heat-resistant mold and cooling it to form a porous hydrophilic bio-wax slow-release carbon source.

[0030] Example 2 A porous hydrophilic bio-wax slow-release carbon source, comprising the following raw materials by weight: 40 parts of beeswax, 40 parts of montan wax, and 80 parts of a pore-forming agent solution, wherein the pore-forming agent solution is a polyvinyl pyrrolidone aqueous solution with a mass concentration of 90 g / L; the preparation method of the porous hydrophilic bio-wax slow-release carbon source adopts the following technical scheme: S61, adding beeswax with a particle size of 0.5-1 cm and montan wax with a particle size of 0.5-1 cm into a reactor respectively according to their mass fractions, heating the reactor in a water bath to 90° C., so that the beeswax and montan wax are completely melted into a liquid state, to obtain liquid A; S62, adding the pore-forming agent solution to liquid A according to the mass fraction, performing aeration treatment to increase the amount of internal bubbles, with an aeration rate of 250 L / min and an aeration time of 8 minutes to obtain a mixed solution; S63, pouring the mixed solution into a heat-resistant mold and cooling it to form a porous hydrophilic bio-wax slow-release carbon source.

[0031] Example 3 A porous hydrophilic bio-wax slow-release carbon source, comprising the following raw materials by weight: 20 parts of beeswax, 40 parts of montan wax, and 40 parts of a pore-forming agent solution, wherein the pore-forming agent solution is a polyvinyl pyrrolidone aqueous solution with a mass concentration of 100 g / L; the preparation method of the porous hydrophilic bio-wax slow-release carbon source adopts the following technical scheme: S61, adding beeswax with a particle size of 0.5-1 cm and montan wax with a particle size of 0.5-1 cm into a reactor respectively according to their mass fractions, heating the reactor in a water bath to 85° C., so that the beeswax and montan wax are completely melted into a liquid state, to obtain liquid A; S62, adding the pore-forming agent solution to liquid A according to the mass fraction, performing aeration treatment to increase the amount of internal bubbles, with an aeration rate of 250 L / min and an aeration time of 5 minutes to obtain a mixed solution; S63, pouring the mixed solution into a heat-resistant mold and cooling it to form a porous hydrophilic bio-wax slow-release carbon source, which is labeled as S1.

[0032] Example 4 A porous hydrophilic biowax slow-release carbon source, comprising the following raw materials by weight: 20 parts of beeswax, 40 parts of montan wax, 40 parts of pore-forming agent solution, and 6 parts of activated carbon with a particle size of 200-325 meshes, wherein the pore-forming agent solution is a polyvinyl pyrrolidone aqueous solution with a mass concentration of 100 g / L; The method for preparing the porous hydrophilic biowax slow-release carbon source adopts the following technical scheme: S61, adding beeswax with a particle size of 0.5-1 cm and montan wax with a particle size of 0.5-1 cm into a reactor respectively according to their mass fractions, heating the reactor in a water bath to 85° C., so that the beeswax and montan wax are completely melted into a liquid state, to obtain liquid A; S62, adding the pore-forming agent solution and activated carbon to liquid A according to their weight fractions, and performing aeration treatment to increase the amount of internal bubbles. The aeration rate is 250 L / min, and the aeration time is 5 minutes to obtain a mixed solution; S63, pouring the mixed solution into a heat-resistant mold and cooling it to form a porous hydrophilic bio-wax slow-release carbon source, which is labeled as S2.

[0033] Example 5 The same as Example 3, except that: 40 parts of beeswax, 20 parts of montan wax, and 40 parts of pore-forming agent solution.

[0034] Comparative Example 1 The beeswax is heated to 90°C in a water bath to completely melt the beeswax into a liquid state, and then poured into a heat-resistant mold for cooling and casting to obtain a bio-wax slow-release carbon source, which is labeled F.

[0035] Comparative Example 2 The lignite wax is heated to 85° C. in a water bath to completely melt the lignite wax into a liquid state, and then poured into a heat-resistant mold for cooling and casting to obtain a bio-wax slow-release carbon source, which is labeled as H.

[0036] Comparative Example 3 Beeswax and montan wax are mixed in a ratio of 1:2, and the mixture is heated to 85°C in a water bath to completely melt the beeswax and montan wax into a liquid state. The mixture is then poured into a heat-resistant mold and cooled to form a bio-wax slow-release carbon source, which is labeled S.

[0037] Performance testing The bio-wax slow-release carbon sources prepared in Examples 3 to 5 and Comparative Examples 1 to 3 were sampled for subsequent various tests.

[0038] 1. Determination of the porosity of biowax slow-release carbon source For the samples prepared in the above-mentioned Examples 3 to 5 and Comparative Examples 1 to 2, the pore sizes were measured by visual observation and stereomicroscope observation. A certain area of ​​each sample was selected to measure 15 to 20 pore sizes, and the average value was calculated to evaluate the porosity. The test results are shown in FIG. Figure 1 , Figure 2 , Figure 3 , Figure 4 , Figure 5 , Figure 6 , Figure 7 and Figure 8 shown.

[0039] 2. Biowax slow-release carbon source biofilm and water purification test The biowax slow-release carbon source samples prepared in Example 3 and Example 4 and Comparative Examples 1 to 3 were suspended in a lake water at a depth of one meter (Sanjian Lake, the distribution map of the sampling points for Sanjian Lake detection is shown in Fig. Fig.15 The water quality test results are shown in Table 1). The biofilms were naturally formed on days 7, 14 and 21 respectively. Two samples of each type were hung for the subsequent determination of sample biomass and nitrogen and phosphorus removal capacity.

[0040] Table 1 Results of water quality testing at Sanjian Lake 2.1 Microbial growth (1) Extraction of biowax slow-release carbon source film: Take the above biowax slow-release carbon source and cut it into two biowax slow-release carbon source discs with a radius of 0.65 cm and a thickness of 0.5 cm using a hole puncher. After gently rinsing the biomass floating on the surface with distilled water, transfer it to a test tube and add 100 ml of distilled water. Ultrasonicate for 20 minutes to allow the biowax slow-release carbon source to fall off and be removed from the bacterial solution. Repeat this process once, combine the bacterial solutions, and use them to count the number of biofilm colonies.

[0041] (2) Colony count: Prepare beef extract peptone medium plate, take 100ul bacterial solution with a pipette and add it to a centrifuge tube containing 900ul pure water to prepare a bacterial suspension, then dilute it in sequence to obtain a bacterial solution diluted 10 times to 1000 times, take 100uL of the three gradient bacterial solutions diluted 10 times to 1000 times with a pipette onto the plate, spread it evenly, and culture it at 37°C for 24-48h, calculate the colony count, and analyze the biofilm formation of each sample. Make 2 plates for each gradient. The test results are shown in Table 2.

[0042] 2.2 Effect of different biowax slow-release carbon source biofilm-forming time on ammonia nitrogen and total phosphorus removal Using NH 4 Cl and K 2 HPO 4Prepare a solution with an ammonia nitrogen content of 4 mg / L and a total phosphorus content of 0.5 mg / L. The standard for Class V water is Class V water: ammonia nitrogen 2 mg / L, total phosphorus 0.2 mg / L. This study used 2 times the standard ammonia nitrogen concentration of Class V water and 2.5 times the total phosphorus concentration of Class V water for the experiment. Take the solution and the bio-wax slow-release carbon source block after natural biofilm formation, add the bio-wax slow-release carbon source block at a solid-liquid ratio of 1:10 in a 250ml beaker for simulation, and then conduct daily ammonia nitrogen and total phosphorus measurements on the solution every week for 3 consecutive weeks (samples are taken for testing at 9:00 am every day).

[0043] The removal rates of ammonia nitrogen and total phosphorus were (daily concentration - initial concentration) / initial concentration × 100%.

[0044] Ammonia nitrogen test method: 1). Take 5ml of the water sample to be tested and 5ml of pure water, add 1ml of LH-N3 reagent, then add 1ml of LH-N2 reagent, shake well, let stand at room temperature for 10 minutes and then test.

[0045] 2). In the initial interface, select the measurement interface, select "Ammonia Nitrogen Dish Colorimetry", and the instrument will automatically adjust to the ammonia nitrogen dish colorimetry mode. Use a 10mm colorimetric dish for detection and analysis, first use a blank sample to calibrate the blank, and when the value is stable, press the "Blank" key to calibrate, then add the samples to be tested in turn, and read and record when the value is stable.

[0046] Total phosphorus test method: 1). Preheating of the digester: Turn on the power of the digester, select the total phosphorus digestion mode, set the preset temperature to 120℃, and let the instrument heat up automatically. When the instrument reaches the set temperature, it will buzz and alarm. Press any button to stop the alarm. At this time, the digester is in use.

[0047] 2). Take 4 ml of the water sample to be tested and 4 ml of pure water and add them into the digestion tube, add 1 ml of potassium persulfate, shake well, digest at 120°C for 30 min, air cool for 2 min, cool in a water bath for 2 min, then add 1 ml of LH-P1 reagent and 1 ml of LH-P2 reagent, shake well, and let stand for 10 min.

[0048] 3). In the initial interface, select the measurement interface, select "Total Phosphorus Colorimetric", and the instrument will automatically adjust to the total phosphorus colorimetric mode. After the water bath is cooled, select a 30mm colorimetric dish for detection and analysis. First, use a blank sample to calibrate the blank. When the value is stable, press the "Blank" key to calibrate, then add the samples to be tested in turn, and read and record when the value is stable.

[0049] Table 2 Biomass From the data in Table 2, it can be seen that the CFU of S2 was the highest among the above-mentioned test samples at 7 and 14 days of biofilm formation, which were 215.23 and 631.97 respectively. The CFU of F and H at 7 and 14 days were significantly lower than that of S2, but at 21 days of biofilm formation, the CFU was higher than that of S2, at which time F was about 4.5 times that of S2, at 2097.63; and H was about 2 times that of S2, at 855.15.

[0050] Figure 3 This is the surface pore map of the bio-wax slow-release carbon source prepared in Example 3. Figure 4 This is an enlarged view of the surface pores of the biowax slow-release carbon source prepared in Example 3; Figure 3 and Figure 4 , it can be seen that the pores are mostly circular, with diameters basically between 0.05-0.10 mm, uniform in size, evenly distributed, and the pore surface accounts for a large proportion, which is 100% of the surface.

[0051] Figure 5 This is the surface pore map of the bio-wax slow-release carbon source prepared in Example 4. Figure 6 This is an enlarged view of the surface pores of the bio-wax slow-release carbon source prepared in Example 4; Figure 5 and Figure 6 It can be seen that the diameter is basically around 0.08-0.13mm. The hole shape is regular, the holes are deep, the distribution is uniform and the number of pores is large, accounting for 100% of the surface area.

[0052] Figure 7 This is the surface pore map of the bio-wax slow-release carbon source prepared in Example 5. Figure 8 This is an enlarged view of the surface pores of the bio-wax slow-release carbon source prepared in Example 5; Figure 7 and Figure 8 It can be seen that the surface pores are unevenly distributed. The dense pores are mostly round, with a diameter of about 0.10 mm, uniform in size, accounting for 40% of the total area; the sparse pores are round, and the pore layer is thin, with a diameter of about 0.15 mm, accounting for 60% of the total area.

[0053] Fig. 9 The ammonia nitrogen removal rate curve of the biowax slow-release carbon source biofilm formed on 7 days was prepared for Example 3 and Example 4 and Comparative Examples 1 to Comparative Examples 3. Fig.10 A 14-day ammonia nitrogen removal rate curve of biowax slow-release carbon source biofilm formation was prepared for Example 3 and Example 4 and Comparative Examples 1 to Comparative Examples 3; Fig.11 Ammonia nitrogen removal rate curves of 21-day biowax slow-release carbon source biofilm formation were prepared for Example 3 and Example 4 as well as Comparative Examples 1 to 3.

[0054] from Figure 9-11It can be seen that the ammonia nitrogen removal rates of S1 and S2 were higher when the biofilm was formed on the 14th and 21st days. It can be seen from the three time periods that on the 7th day, F had the best removal effect, which was maintained at about 45%, the removal rate of S1 was maintained at about 35%, and S2 showed an obvious increase in ammonia nitrogen instead of a decrease; on the 14th day, the ammonia nitrogen removal rates of all biowaxes reached the highest, among which S1 reached a removal rate of 98%, S2 reached a removal rate of 92%, and the removal rates of F, H, and S were concentrated at about 70%; on the 21st day, the ammonia nitrogen removal rate of S2 was the highest among all biowaxes, and the change curve was tortuous. The removal rate decreased from the second to the fifth day and from the sixth to the seventh day in the continuous measurement, among which the removal rate on the second day was the highest, reaching 70%, and the change curves of the removal rates of the remaining biowaxes after 21 days of biofilm formation showed a law of increasing first and then decreasing.

[0055] Fig.12 The total phosphorus removal rate curve of the biowax slow-release carbon source biofilm formed in 7 days is prepared for Example 3 and Example 4 and Comparative Examples 1 to Comparative Examples 3. Fig.13 The total phosphorus removal rate curve of the biowax slow-release carbon source biofilm formed on 14 days was prepared for Example 3 and Example 4 as well as Comparative Examples 1 to 3. Fig.14 The total phosphorus removal rate curve of 21 days of biowax slow-release carbon source biofilm formation was prepared for Example 3 and Example 4 as well as Comparative Examples 1 to 3.

[0056] from Figure 12-14 It can be seen that when the biofilm formation time is 7 days and 21 days, the total phosphorus removal rate of other biowaxes except S2 does not increase but decreases, and obviously shows negative values. It can be seen from the three time periods that when the biofilm formation time is 7 days, 14 days and 21 days, the removal effect of S2 is better than that of other biowaxes: on the 7th day, the total phosphorus removal rate of S2 is as high as 78%; on the 14th day, the total phosphorus removal rates of S1 and S2 fluctuate at 30%; on the 21st day, the removal rate of S2 is as high as 77%.

[0057] The above embodiments are only used to explain the technical solutions of the present application rather than to limit them. Although the above embodiments provide a specific description of the present application, relevant technical personnel should understand that the specific implementation methods of the present application can still be modified or replaced by equivalents, and any modifications and equivalent replacements that do not depart from the spirit and scope of the present application should be included in the protection scope of the present application.

Claims

1. A porous hydrophilic biowax slow-release carbon source, characterized in that: The preparation method comprises the following raw materials by weight: 20-40 parts of beeswax, 20-40 parts of montan wax and 20-80 parts of pore-forming agent solution.

2. A porous hydrophilic biowax slow-release carbon source according to claim 1, characterized in that: The mass ratio of the beeswax, montan wax and pore-forming agent solution is 1:2:

2.

3. The porous hydrophilic biowax slow-release carbon source according to claim 1, characterized in that: The pore-forming agent solution is a polyvinyl pyrrolidone aqueous solution with a mass concentration of 90-110 g / L or a polyethylene glycol aqueous solution with a mass concentration of 60-70 g / L.

4. The porous hydrophilic biowax slow-release carbon source according to claim 1, characterized in that: The pore diameter of the biowax slow-release carbon source is 0.05-0.10 mm.

5. The porous hydrophilic biowax slow-release carbon source according to claim 1, characterized in that: It also includes 4-8 parts of activated carbon with a particle size of 200-325 meshes.

6. A method for preparing a porous hydrophilic biowax slow-release carbon source as claimed in any one of claims 1 to 4, characterized in that: The following steps are involved: S61, adding beeswax with a particle size of 0.5-1 cm and montan wax with a particle size of 0.5-1 cm into a reactor respectively according to their mass fractions, heating in a water bath to completely melt the beeswax and montan wax into a liquid state, and obtaining liquid A; S62, adding the pore-forming agent solution to the liquid A according to the mass fraction and mixing evenly to obtain a mixed solution; S63, pouring the mixed solution into a heat-resistant mold and cooling it to form a porous hydrophilic bio-wax slow-release carbon source.

7. A method for preparing a porous hydrophilic biowax slow-release carbon source as claimed in any one of claims 1 to 5, characterized in that: The following steps are involved: S71. Add beeswax with a particle size of 0.5-1 cm and montan wax with a particle size of 0.5-1 cm into a reactor according to their mass fractions, and heat the reactor in a water bath to completely melt the beeswax and montan wax into a liquid state to obtain liquid A. S72, adding the pore-forming agent solution and activated carbon to liquid A in order according to the mass fractions, and mixing them evenly to obtain a mixed solution; S73, pouring the mixed solution into a heat-resistant mold and cooling it to form a porous hydrophilic bio-wax slow-release carbon source.

8. A method for preparing a porous hydrophilic biowax slow-release carbon source according to claim 6 or 7, characterized in that: In step S61 or S71, the water bath is heated to a temperature of 80-90°C.

9. The method for preparing a porous hydrophilic biowax slow-release carbon source according to claim 6 or 7, characterized in that: In step S62 or S72, the uniform mixing method is stirring at a mechanical stirring speed of 600 r / min for 4-8 minutes or using aeration treatment to increase the amount of internal bubbles, the aeration rate is 250 L / min, and the aeration time is 4-8 minutes.

10. An application of the porous hydrophilic biowax slow-release carbon source as claimed in any one of claims 1 to 5 in the treatment of eutrophication of water bodies, characterized in that: The porous hydrophilic biowax slow-release carbon source is suspended in the water body to promote the degradation of ammonia nitrogen and total phosphorus by microorganisms.

Images