Compost high-efficiency decay-promoting microbial inoculum as well as preparation method and application thereof

Abstract

The invention relates to a high-efficiency compost decay-promoting microbial inoculum as well as a preparation method and application thereof, and belongs to the technical field of biological agriculture and related industries. The microbial agent is prepared from the following bacterial strains: bacillus licheniformis DY-1, bacillus massiliensis MS, bacillus aryabhattai MB and gas-producing paenibacillus XWS-70; the viable count of each strain is greater than or equal to 5 * 10 < 8 > CFU / g; the total viable count of the microbial inoculum is greater than or equal to 109 CFU / g. The preparation method comprises the steps of strain activation, strain amplification culture, strain powder preparation and strain compounding. The invention relates to application in rapid maturing and composting of organic wastes. The invention has the following advantages: no antagonism is generated among the four strains, the degradation of cellulose can be obviously accelerated, and the degree of humus is improved.

Description

Technical Field

[0001] The invention belongs to the technical field of biological agriculture and related industries, and specifically relates to a composting efficient decay-promoting bacterial agent and a preparation method and application thereof. Background Art

[0002] With the intensive production of agriculture and animal husbandry in my country, a large amount of agricultural organic waste has accumulated. Composting is the main way to effectively treat organic waste and make use of it. It can convert organic waste into organic fertilizer, decompose high-molecular substances such as cellulose that are difficult to use, and promote the formation of usable small molecules such as fulvic acid (FA) and humic acid (HA). Therefore, accelerating the degradation of cellulose into stable humus is the key issue to solve the high-quality composting problem.

[0003] There are mainly physical, chemical and biological technologies for the treatment of organic waste at home and abroad. At present, biotechnology is often used for high-quality production of organic fertilizers, and the efficiency and quality of composting are improved by adding microbial agents. The existing technology focuses on single strains such as cellulose degradation, and there are few composite agents that can efficiently solve high-quality problems. Therefore, developing a composite microbial agent that can quickly mature agricultural organic waste and reduce resource waste is an effective way to achieve high-quality production. Summary of the invention

[0004] The first object of the present invention is to disclose a high-efficiency composting and decay-promoting bacterial agent.

[0005] The second object of the present invention is to disclose a method for preparing the above-mentioned high-efficiency decay-promoting bacteria agent.

[0006] The third object of the present invention is to disclose the application of the above-mentioned high-efficiency decay-promoting bacteria agent.

[0007] The objective of the present invention is achieved through the following technical solutions: A high-efficiency composting and decay-promoting bacterial agent, wherein: the bacterial agent is composed of Bacillus licheniformis ( Bacillus lichen-like )CGMCC No.32099, Ureibacillus massiliensis CGMCC No.32598, Bacillus aeruginosa ( Bacillus aryabhattai )CGMCC No.32597 and Paenibacillus odorifer CGMCC No.33451 composition; The mass ratio between the strains is one of A, B, C, D, E or F, wherein: A is Bacillus licheniformis: Ureibacillus massiliensis :Bacillus agglutinosa: Paenibacillus fragrant =1.5:3:2:1; B is Bacillus licheniformis: Ureibacillus massiliensis:Bacillus agglutinosa: Paenibacillus fragrant =1.5:1.5:2:1; C is Bacillus licheniformis: Ureibacillus massiliensis :Bacillus agglutinosa: Paenibacillus fragrant =1.5:1.5:3:1; D is Bacillus licheniformis: Ureibacillus massiliensis :Bacillus agglutinosa: Paenibacillus fragrant =1.5:2:3:1; E is Bacillus licheniformis: Ureibacillus massiliensis :Bacillus agglutinosa: Paenibacillus fragrant =1.5:2:1:1; or F is Bacillus licheniformis: Ureibacillus massiliensis :Bacillus agglutinosa: Paenibacillus fragrant =1.5:3:1:1; The number of viable cells of each strain was ≥5×10 8 CFU / g; the total viable count of the bacterial agent is greater than or equal to 10 9 CFU / g.

[0008] The method for preparing the high-efficiency compost-promoting microbial agent described in the above technical solution comprises the following steps: (1) Strain activation: Bacillus licheniformis, Ureibacillus massiliensis , Bacillus argyi, Paenibacillus fragrant were inoculated into LB liquid medium and cultured to the logarithmic growth phase to obtain Bacillus licheniformis, Ureibacillus massiliensis , Bacillus argyi, Paenibacillus odorifer Seed liquid; (2) Expansion culture of bacterial strains: The Bacillus licheniformis obtained in step (1) Ureibacillus massiliensis , Bacillus argyi, Paenibacillus odorifer The seed solution was transferred to a new LB liquid medium at a 10% (v / v) inoculation rate, and the corresponding bacterial solution was obtained by expansion culture; (3) Preparation of bacterial powder: The Bacillus licheniformis obtained in step (2) Ureibacillus massiliensis , Bacillus argyi, Paenibacillus odorifer The bacterial solution was centrifuged at 6000 rpm for 5 min at 4°C to collect the bacterial precipitate, which was freeze-dried to prepare bacterial powder of the four strains; (4) Compounding of bacterial strains: The licheniformis obtained in step (3) is detected Ureibacillus massiliensis , Bacillus argyi, Paenibacillus odorifer The number of live bacteria contained in the bacterial powder is Ureabacillus from Marseille , Bacillus argyi, Paenibacillus odorifer By mixing according to the mass ratio described in claim 1, a high-efficiency compost-promoting bacteria agent can be obtained.

[0009] The preparation method described in the above technical solution, wherein: the culture conditions in steps (1) and (2) are: Bacillus licheniformis DY-1 was cultured at 30°C and 180 rpm in a shaking incubator for 13–15 h; Bacillus massiliense MS was cultured at 30°C and 180 rpm in a shaking incubator for 12-17 hours; Bacillus aeruginosa MB was cultured at 30°C, 180 rpm in a shaking incubator for 13-16 h; Paenibacillus aerogenes XWS-70 was cultured at 30°C and 180 rpm in a shaking incubator for 12-15 hours.

[0010] The preparation method described in the above technical solution, wherein: the number of viable bacteria in the bacterial powder in step (4) is: The number of viable cells of each strain was ≥5×10 8 CFU / g; the number of viable bacteria in the mixed agent is ≥10 9 CFU / g.

[0011] The application of the high-efficiency composting-promoting bacteria agent described in the above technical solution in the rapid decomposition and composting of organic waste.

[0012] Bacillus licheniformis ( Bacillus licheniformis ) CGMCC No.32099 (hereinafter referred to as: Bacillus licheniformis DY-1) was screened from mushroom residue and grew on beef extract peptone agar medium. The colonies were light yellow, opaque, oval, slightly convex, and the bacteria were single or paired, rod-shaped, and had spores.

[0013] Ureibacillus massiliensis CGMCC No.32598 (hereinafter referred to as: Bacillus Marseille MS) was screened from mushroom residue and grew on beef extract peptone agar medium. The colonies were light yellow, translucent, round, slightly convex, smooth and with neat edges, moist, rod-shaped, and with spores.

[0014] Bacillus arguta Bacillus aryabhattai ) CGMCC No.32597 (hereinafter referred to as: Bacillus arnoldii MB) was screened from mushroom residue and grew on beef extract peptone solid culture medium. The colonies were light yellow, opaque, round, slightly without protrusions, smooth surface and neat edges, moist, rod-shaped, and had spores.

[0015] Paenibacillus odoriferCGMCC No.33451 (hereinafter referred to as: Paenibacillus aerogenes XWS-70) was screened from mushroom residues and grew on beef extract peptone agar medium. The colonies were milky white, translucent, round, slightly convex, with smooth surface and neat edges, moist, smooth texture, and spores.

[0016] The present invention has the following beneficial effects: 1. The present invention screened and obtained a strain of Bacillus licheniformis DY-1, which has excellent β-glucosidase activity.

[0017] 2. The present invention screened and obtained a strain of Bacillus Massimoides MS, which has excellent CMC enzyme activity.

[0018] 3. The present invention screened and obtained a strain of Bacillus agni MB, which has excellent filter paper enzyme activity.

[0019] 4. The present invention screened and obtained a gas-producing Bacillus XWS-70, which has excellent CMC enzyme activity.

[0020] 5. The present invention is to Bacillus licheniformis DY-1 ( Bacillus licheniformis DY-1), Bacillus massiliense MS ( Ureibacillus massiliensis MS), Bacillus arguta MB ( Bacillus aryabhattai MB), Paenibacillus aerogenes XWS-70 ( Paenibacillus odorifer XWS-70) were mixed and compounded, and it was found that the four strains not only had no antagonistic effect on each other, but also could significantly accelerate the degradation of cellulose to form stable humus. After actual testing, the CMC enzyme activity, β-glucosidase activity and filter paper enzyme activity of the four strains after compounding reached 69.74±5.42 U / mL, 70.13±7.11 U / mL and 65.51±7.31 U / mL, respectively.

[0021] Strain deposit information: 1. The Bacillus licheniformis DY-1 of the present invention is classified and named as Bacillus licheniformis Bacillus lichen-like It has been deposited in the General Microbiology Center of China Culture Collection Administration. The address of the depository is No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The deposit number is CGMCC NO.32099 and the deposit date is September 29, 2024.

[0022] 2. The Bacillus Massimo MS of the present invention is classified and named Ureibacillus massiliensisIt has been deposited in the General Microbiology Center of China Microorganism Culture Collection (CGMCC). The address of the depository is No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The deposit number is CGMCC NO.32598 and the deposit date is November 12, 2024.

[0023] 3. The present invention's Bacillus agni MB is classified and named Bacillus agni Bacillus aryabhattai It has been deposited in the General Microbiology Center of China Microorganism Culture Collection (CGMCC). The address of the depository is No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The deposit number is CGMCC NO.32597 and the deposit date is November 12, 2024.

[0024] 4. The aerogenes bacillus XWS-70 of the present invention is classified and named Paenibacillus odorifer It has been deposited in the General Microbiology Center of China Microorganism Culture Collection (CGMCC). The address of the depository is No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing. The deposit number is CGMCC NO.33451 and the deposit date is January 17, 2025. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 It is the process of strain screening and composite bacterial agent preparation; Figure 2 is the evolutionary tree of strain DY-1; Figure 3 is the evolutionary tree of strain MS; Figure 4 is the evolutionary tree of strain MB; Figure 5 is the evolutionary tree of strain XWS-70; Figure 6 These are the results of pairwise antagonism experiments between strains. DETAILED DESCRIPTION

[0026] To facilitate the understanding of the technical solution of the present invention, the following further describes a high-efficiency composting and decay-promoting bacterial agent, a preparation method thereof, and an application of the present invention in conjunction with specific embodiments.

[0027] The process of strain screening and preparation of high-efficiency composting-promoting bacteria is shown in Figure 1 The specific preparation process is as described in the examples.

[0028] Example 1: Isolation of strains: Take 10 g of mushroom residue and put it into 50 mL of sterilized water. Shake it at 30°C and 180 rpm for 30 minutes. Let it stand for 30 minutes. Take 1 mL of the supernatant and dilute it to 10 with sterilized water. -1 , and make 10-fold gradient dilutions continuously. Take 0.5 mL of the suspension with different dilution multiples and spread it on the surface of beef extract peptone agar medium, culture it at 30℃ for 24-36 h, and pick strains with different morphologies for streaking culture in CMC-Na medium, β-glucoside medium and filter paper strip medium according to the morphology and color changes of the strains, and pick strains with good growth for low temperature storage.

[0029] The culture medium and its formulation in the above embodiment are as follows: Beef extract peptone agar medium: peptone: 10 g, beef extract: 5 g, NaCl: 5 g, agar: 20 g, distilled water: 1000 mL, PH: 7.2; CMC-Na medium: CMC-Na: 15 g, MgSO 4 7H 2 O: 0.5 g, yeast extract: 1.0 g, KH 2 PO 4 :1.0 g,NH 4 NO 3 : 1.0 g, agar: 20 g, distilled water: 1000 mL, pH: 6.8-7.2.

[0030] β-Glucoside medium: salicin: 5 g, MgSO 4 7H 2 O: 0.5 g, yeast extract: 1.0 g, KH 2 PO 4 :1.0g,NH 4 NO 3 : 1.0 g, agar: 20 g, distilled water: 1000mL, pH: 6.8-7.2.

[0031] Filter paper strip culture medium: Filter paper strips (1×2 cm): 30 strips, MgSO4·7H 2 O: 0.5 g, yeast extract: 1.0 g, KH 2 PO 4 :1.0 g,NH 4 NO 3 : 1.0 g, agar: 20 g, distilled water: 1000mL, pH: 6.8-7.2.

[0032] Example 2: Screening and identification of strains: 1. Screening of strains: The strains were screened by cellulase activity. The specific steps are as follows: 1.1. Seed solution preparation: Use a sterile inoculation loop to pick a single colony of the strain that grows well on the solid culture medium from the slant, inoculate it into LB liquid culture medium, place it at 30°C, shake it at 180 rpm, and culture it to the logarithmic growth phase to prepare the seed solution.

[0033] 1.2. Screening of cellulose-degrading bacteria: The various sub-liquids were inoculated into CMC-Na liquid culture medium, β-glucosidase liquid culture medium and filter paper strip liquid culture medium at a 10% (v / v) inoculation rate, and cultured continuously at 30°C and 180 rpm for 7 days. The activities of CMC enzyme, β-glucosidase and filter paper enzyme were determined by DNS method.

[0034] The culture medium and formula used in the above screening steps are as follows: LB liquid medium: peptone: 10 g, yeast powder: 5 g, NaCl: 5 g, pH: 7.2; CMC-Na liquid medium: CMC-Na: 5 g, MgSO 4 7H 2 O: 0.5 g, yeast extract: 1.0 g, KH 2 PO 4 :1.0g,NH 4 NO 3 : 1.0 g, distilled water: 1000 mL, pH: 6.8-7.2.

[0035] β-Glucoside liquid medium: salicin: 5 g, MgSO 4 7H 2 O: 0.5 g, yeast extract: 1.0 g, KH 2 PO 4 :1.0 g,NH 4 NO 3 : 1.0 g, distilled water: 1000 mL, pH: 6.8-7.2.

[0036] Filter paper strips Liquid medium: Filter paper strips (1 × 2 cm): 30 strips, MgSO 4 7H 2 O: 0.5 g, yeast extract: 1.0 g, KH 2 PO 4 :1.0 g,NH 4 NO 3 : 1.0 g, distilled water: 1000 mL, pH: 6.8-7.2.

[0037] According to the above screening steps, 4 strains with strong CMC enzyme activity (U / mL), β-glucosidase activity (U / mL) and filter paper enzyme activity (U / mL) were screened. The decay-promoting ability of each strain is shown in Table 1: Table 1 Cellulase degradation activity of each strain

[0038] 2. Identification of bacterial strains: 2.1. Physiological and biochemical tests: 2.1.1. Inoculate the four strains obtained in step 1 on beef extract peptone agar medium, culture at 30°C for 48 h, observe and record the colony growth and morphology, and observe and record the bacterial morphology under a microscope.

[0039] Bacillus licheniformis DY-1 was screened from mushroom residue and grew on beef extract peptone agar medium. The colonies were light yellow, opaque, oval, slightly convex, and the bacteria were single or paired, rod-shaped, and had spores.

[0040] Bacillus Massimo MS was screened from mushroom residue and grew on beef extract peptone agar medium. The colonies were light yellow, translucent, round, slightly protruding, smooth and with neat edges, moist, rod-shaped, and with spores.

[0041] Bacillus arnoldii MB was screened from mushroom residue and grew on beef extract peptone solid medium. The colonies were light yellow, opaque, round, slightly without protrusions, smooth and with neat edges, moist, rod-shaped, and with spores.

[0042] The aerogenes Paenibacillus XWS-70 was screened from mushroom residue and grew on beef extract peptone agar medium. The colonies were milky white, translucent, round, slightly convex, with smooth surface and neat edges, moist, smooth texture, and spores.

[0043] 2.1.2. Use bacterial multiphase identification detection method to conduct physiological and biochemical tests on each strain: The specific operations are as follows: (1) Reagent strips used: API 50CH reagent strip, API 20E reagent strip (2) Supporting culture medium: API 50 CHB / E medium, API 0.85% NaCl medium (3) Preparation of bacterial suspension: Use an inoculation tool to pick an appropriate amount of colonies from the plate and place them in API 50 CHB / E medium or API 0.85% NaCl medium, grind them carefully to make a uniform bacterial suspension. (4) Prepare the training environment: Prepare a culture box and pour about 5 mL of distilled water into the honeycomb of the dish to create a wet chamber. (5) Inoculation reagent strips: Place the API 50CH reagent strip or API 20E reagent strip in the culture box and use a pipette to add the bacterial suspension into the small tube of the reagent strip. The bacterial suspension should fill the tube and cup of the CIT, VP and GEL tubes in the API 20E reagent strip. 2 S and URE tubes require the addition of mineral oil to create an anaerobic environment.

[0044] (6) Cultivation: Place the inoculated test strips in an incubator and incubate at 36°C ± 2°C for 18-24 hours.

[0045] (7) Interpretation of results: Observe the color changes of each small tube on the test strip and refer to the instructions of the test strip to determine whether it is positive, weakly positive or negative.

[0046] The physiological and biochemical experimental results of the four strains obtained are shown in Table 2.

[0047] Table 2 Physiological and biochemical characteristics of strains

[0048] Note: “+”: positive; “w”: weak positive; “-”: negative 2.2 Molecular Validation: The four strains were inoculated in LB liquid medium, cultured at 30°C in a shaking incubator for 24 hours, and centrifuged at 12000rpm to collect the bacteria. DY-1, MS, MB and XWS-70 were extracted using the Ezup column bacterial genomic DNA extraction kit. PCR amplification of strains DY-1, MS, MB and XWS-70 was performed using primers 27F and 1492R; the nucleotide sequences of the primers are as follows: 27F: 5'-AGAGTTTGATCCTGGCTCAG-3' 1492R:5'-AGAGTTTGATCCTGGCTCAG-3' After purification, the PCR product was sent to Shanghai Biotechnology Co., Ltd. for sequencing, and the sequencing results were compared with the sequences in the NCBI website database for homology analysis. Figure 2 The evolutionary tree of strain MS is shown in Figure 3 The evolutionary tree of strain MB is shown in Figure 4 The evolutionary tree of strain XWS-70 is shown in Figure 5 shown.

[0049] Finally, the physiological and biochemical characteristics of the four strains were combined with molecular verification to confirm that the four strains DY-1, MS, MB, and XWS-70 involved in the present invention were Bacillus licheniformis DY-1 ( Bacillus licheniformis DY-1), Bacillus massiliense MS ( Ureibacillus massiliensis MS), Bacillus arguta MB ( Bacillus aryabhattai MB), Paenibacillus aerogenes XWS-70 ( Paenibacillus odorifer XWS-70).

[0050] Embodiment 3: Compound of high-efficiency decomposing bacteria agent: 1. Antagonism experiment: The 4 strains were tested for antagonism on solid culture medium using the filter paper method. The bacteria on the plate were the base bacteria, and the bacteria on the filter paper were the test bacteria. The growth of the base bacteria and the test bacteria was observed. If there is no inhibition zone between the two bacteria, it means there is no antagonism. Otherwise, it means there is antagonism between the strains. Figure 6 As shown, there is no antagonism between these four strains.

[0051] 2. Compounding of strains: The obtained 4 decay-promoting strains were inoculated into LB liquid medium and cultured in a shaking table until the logarithmic growth phase to obtain seed solutions of Bacillus licheniformis DY-1, Bacillus masseille MS, Bacillus alteiricus MB, and Paenibacillus aerogenes XWS-70 (the specific method was as follows: Bacillus licheniformis DY-1 was cultured in a shaking table at 30°C and 180 rpm for 14 hours; Bacillus masseille MS was cultured in a shaking table at 30°C and 180 rpm for 15 hours; Bacillus alteiricus MB was cultured in a shaking table at 30°C and 180 rpm for 14 hours, and Paenibacillus aerogenes XWS-70 was cultured in a shaking table at 30°C and 180 rpm for 15 hours. rpm shaking culture for 13 hours to obtain seed liquid of four strains); inoculate and expand the culture and then freeze-dry (the specific method is: the seed liquid of Bacillus licheniformis DY-1, Bacillus massiense MS, Bacillus alteiricus MB, and Bacillus aeruginosa XWS-70 is transferred to a new LB liquid culture medium at an inoculation rate of 10% (v / v), Bacillus licheniformis DY-1 is cultured at 30°C, 180 rpm shaking culture for 14 hours; Bacillus massiense MS is cultured at 30°C, 180 rpm shaking culture for 15 hours; Bacillus alteiricus MB is cultured at 30°C, 180 rpm shaking culture for 14 hours; Bacillus alteiricus MB is cultured at 30°C, 180 rpm shaking culture for 13 hours, and the corresponding bacterial liquid is expanded; the obtained four bacterial liquids are centrifuged at 4°C, 6000 rpm for 5 minutes to collect the bacterial precipitate, freeze-dried, and the bacterial powder of the four strains is prepared), and the bacterial powder (viable cell count ≥5×10 8CFU / g); compounded according to the following mass ratio: (Bacillus licheniformis DY-1: Bacillus massiliense MS: Bacillus agglomerans MB: Paenibacillus aerogenes XWS-70) = A: (1.5:3:2:1); B: (1.5:1.5:2:1); C: (1.5:1.5:3:1); D: (1.5:2:3:1); E: (1.5:2:1:1); F: (1.5:3:1:1), the compound combinations were 6, inoculated into CMC-Na medium, β-glucoside medium and filter paper strip medium, respectively, for the determination of CMC enzyme activity, β-glucosidase activity and filter paper enzyme activity. The results are shown in Table 3. The results in Table 3 show that the CMC enzyme activity of combination A is higher than that of other combinations, the β-glucosidase of combination B is higher than that of other combinations, and the filter paper enzyme activity of combination C is higher than that of other combinations; the CMC enzyme activity and filter paper enzyme activity of combination D are higher than those of other combinations, and the three enzyme activities of combinations E and F are relatively high, higher than the CMC enzyme activity, β-glucosidase activity and filter paper enzyme activity of the compound decay-promoting agent of CN118421517A.

[0052] Table 3 Cellulase activity of the composite bacterial agent

[0053] The above description is only a preferred embodiment of the present invention and does not impose any formal or substantial limitation on the present invention. Any technician familiar with the profession can make use of the technical contents disclosed above without departing from the scope of the technical solution of the present invention, and any slight changes, modifications and evolutions made by equivalent changes are all equivalent embodiments of the present invention; at the same time, any changes, modifications and evolutions of any equivalent changes made to the above embodiments based on the essential technology of the present invention still fall within the scope of the technical solution of the present invention.

Claims

1. A high-efficiency composting and decay-promoting bacterial agent, characterized in that: The bacterial agent is composed of Bacillus licheniformis ( Bacillus licheniformis )CGMCC No.32099, Ureibacillus massiliensis CGMCC No.32598, Bacillus aeruginosa ( Bacillus aryabhattai )CGMCC No.32597 and Paenibacillus odorifer CGMCC No.33451 composition; The mass ratio between the strains is one of A, B, C, D, E or F, wherein: A is Bacillus licheniformis: Ureibacillus massiliensis :Bacillus agglutinosa: Paenibacillus odorifer =1.5:3:2:1; B is Bacillus licheniformis: Ureibacillus massiliensis :Bacillus agglutinosa: Paenibacillus odorifer =1.5:1.5:2:1; C is Bacillus licheniformis: Ureibacillus massiliensis :Bacillus agglutinosa: Paenibacillus odorifer =1.5:1.5:3:1; D is Bacillus licheniformis: Ureibacillus massiliensis :Bacillus agglutinosa: Paenibacillus odorifer =1.5:2:3:1; E is Bacillus licheniformis: Ureibacillus massiliensis :Bacillus agglutinosa: Paenibacillus odorifer =1.5:2:1:1; or F is Bacillus licheniformis: Ureibacillus massiliensis :Bacillus agglutinosa: Paenibacillus odorifer =1.5:3:1:1; The number of viable cells of each strain was ≥5×10 8 CFU / g; the total viable count of the bacterial agent is greater than or equal to 10 9 CFU / g.

2. The method for preparing the high-efficiency compost-promoting microbial agent according to claim 1, comprising the following steps: (1) Strain activation: Bacillus licheniformis, Ureibacillus massiliensis , Bacillus argyi, Paenibacillus odorifer were inoculated into LB liquid medium and cultured to the logarithmic growth phase to obtain Bacillus licheniformis, Ureibacillus massiliensis , Bacillus argyi, Paenibacillus odorifer Seed liquid; (2) Expansion culture of bacterial strains: The Bacillus licheniformis obtained in step (1) Ureibacillus massiliensis , Bacillus argyi, Paenibacillus odorifer The seed solution was transferred to a new LB liquid medium at a 10% (v / v) inoculation rate, and the corresponding bacterial solution was obtained by expansion culture; (3) Preparation of bacterial powder: The Bacillus licheniformis obtained in step (2) Ureibacillus massiliensis , Bacillus argyi, Paenibacillus odorifer The bacterial solution was centrifuged at 6000 rpm for 5 min at 4°C to collect the bacterial precipitate, which was freeze-dried to prepare bacterial powder of the four strains; (4) Compounding of bacterial strains: Detection of the Bacillus licheniformis obtained in step (3), Ureibacillus massiliensis , Bacillus argyi, Paenibacillus odorifer The number of live bacteria contained in the bacterial powder was Ureibacillus massiliensis , Bacillus argyi, Paenibacillus odorifer By mixing according to the mass ratio described in claim 1, a high-efficiency compost-promoting bacteria agent can be obtained.

3. The preparation method according to claim 2, characterized in that: The culture conditions in steps (1) and (2) are: Bacillus licheniformis DY-1 was cultured at 30°C and 180 rpm in a shaking incubator for 13–15 h; Bacillus massiliense MS was cultured at 30°C and 180 rpm in a shaking incubator for 12-17 hours; Bacillus aeruginosa MB was cultured at 30°C, 180 rpm in a shaking incubator for 13-16 h; Paenibacillus aerogenes XWS-70 was cultured at 30°C and 180 rpm in a shaking incubator for 12-15 hours.

4. The preparation method according to claim 2, characterized in that: The number of viable bacteria in the bacterial powder in step (4) is: The number of viable cells of each strain was ≥5×10 8 CFU / g; the number of viable bacteria in the mixed agent is ≥10 9 CFU / g.

5. Use of the high-efficiency compost-promoting bacteria agent according to claim 1 in rapid composting of organic waste.

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