Nucleic acid extraction, detection and analysis integrated instrument

Abstract

The invention discloses a nucleic acid extraction, detection and analysis integrated instrument, and relates to the technical field of biological detection.The nucleic acid extraction, detection and analysis integrated instrument comprises an integrated processing mechanism, funnel-shaped pipes are fixed in two through holes of a mounting rack, electric valves are mounted at the output ends of two connecting pipes, flow sensors are mounted at the output ends of the two electric valves, and the flow sensors are connected with the integrated processing mechanism. A first electric push rod is mounted on the rear surface of the inner wall of the shell, an asbestos wire gauze is arranged at the top of a placement ring, an evaporation pan is arranged at the top of the asbestos wire gauze, an alcohol lamp is arranged at a groove in the top of a placement plate, and centrifugal equipment is arranged at the top of the inner wall of the shell. Substances in some solvents used in the nucleic acid extraction, purification and detection process of the sample can be recycled, so that the utilization rate of resources is improved, and the use efficiency of the nucleic acid extraction, detection and analysis integrated instrument is improved.

Description

Technical Field

[0001] The present invention relates to the field of biological detection technology, and in particular to an integrated instrument for nucleic acid extraction, detection and analysis. Background Art

[0002] Biological detection technology is widely used in the fields of medical treatment, environmental protection, food safety, biomaterials, etc., and can provide important protection for human health and safety. Among them, biological detection in medical field is commonly pathogen detection, and biological detection generally requires steps such as nucleic acid extraction, purification, detection and analysis. Common biological detection equipment is integrated instrument for nucleic acid extraction, detection and analysis.

[0003] Although the existing integrated nucleic acid extraction, detection and analysis instruments for biological detection can extract and detect nucleic acids in samples to obtain the desired structure during actual use, they cannot recycle and reuse some substances in the solvent after use, thereby causing a waste of resources, that is, reducing the use effect of the integrated nucleic acid extraction, detection and analysis instruments, that is, reducing the use efficiency of the integrated nucleic acid extraction, detection and analysis instruments.

[0004] Therefore, it is necessary to propose a new integrated instrument for nucleic acid extraction, detection and analysis in order to solve the above-mentioned problems. Summary of the invention

[0005] The purpose of the present invention is to provide an integrated instrument for nucleic acid extraction, detection and analysis, so as to solve the problem that the existing integrated instrument for nucleic acid extraction, detection and analysis cannot recycle and reuse the components in some used solvents, thereby causing waste of resources, that is, reducing the use effect of the integrated instrument for nucleic acid extraction, detection and analysis, that is, reducing the use efficiency of the integrated instrument for nucleic acid extraction, detection and analysis.

[0006] To achieve the above-mentioned object, the present invention provides the following technical solutions: an integrated instrument for nucleic acid extraction, detection and analysis, comprising an integrated processing mechanism, one side of which is provided with an auxiliary mechanism; The auxiliary mechanism comprises a shell, a mounting frame is installed on the top of the shell, funnel-shaped tubes are fixed inside two through holes of the mounting frame, connecting tubes are installed at the output ends of the two funnel-shaped tubes, electric valves are installed at the output ends of the two connecting tubes, flow sensors are installed at the output ends of the two electric valves, and first round tubes are installed at the output ends of the two flow sensors, a mounting groove is provided on the front surface of the inner wall of the shell, a first electric push rod is installed on the rear surface of the inner wall of the shell, a hollow block is fixed to the telescopic end of the first electric push rod, a test tube is arranged inside the hollow block, a rectangular block is fixed on the top of the inner wall of the shell, a T-block is slidably connected inside the mounting groove, a placing ring is fixed on the side of the T-block close to the rectangular block, an asbestos net is arranged on the top of the placing ring, an evaporating dish is arranged on the top of the asbestos net, a hand-tightening bolt is threaded through the front surface of the shell, a placing plate is fixed on the front surface of the inner wall of the shell, an alcohol lamp is arranged at the top groove of the placing plate, and a centrifugal device is arranged on the top of the inner wall of the shell.

[0007] Preferably, the shell is located between the two limit blocks, the output ends of the two funnel-shaped tubes are movable through the top of the shell, the two flow sensors are respectively installed on the front surface and the rear surface of the rectangular block, and one end of the hand-tightening bolt is threadedly connected to the front surface of the placement ring.

[0008] Preferably, a shell cover is provided on one side of the shell, limiting blocks are fixed on the front surface and the rear surface of the shell, pipe plugs are installed on the tops of the two funnel-shaped tubes, a second round tube is fixedly penetrated through the top of the shell, and a limiting ring is movably sleeved on the outer wall of the evaporating dish, and the limiting ring is fixed on the front surface of the inner wall of the shell.

[0009] Preferably, the integrated processing mechanism includes a workbench, the shell is fixed on one side of the workbench, a plurality of support columns are fixed to the bottom of the workbench, four rectangular grooves are opened on the inner wall of the workbench, and three slide grooves are evenly distributed on the bottom of the inner wall of the workbench.

[0010] Preferably, the inner wall front surface of each rectangular groove and the inner wall front surface of each slide groove are rotatably connected to a threaded rod through a bearing, a rectangular plate is fixed on one side of the workbench near the edge of the front surface, and seven motors are installed on the rear surface of the workbench.

[0011] Preferably, one end of four of the threaded rods close to the motor moves through the rear surfaces of the inner walls of the four rectangular grooves, and one end of the other three threaded rods close to the motor moves through the rear surfaces of the inner walls of the three slide grooves, one end of each threaded rod close to the motor is installed on the output end of each motor, and a placement rack is slidably connected to the interior of each rectangular groove.

[0012] Preferably, one end of four of the threaded rods are respectively threaded through one side of four placement racks close to the motor, a liquid storage tube is provided inside each of the placement racks, a tube cover is provided on the top of each of the liquid storage tubes, a slider is slidably connected inside each of the slide grooves, and one end of the other three threaded rods are respectively threaded through one side of three sliders close to the motor, a placement table is fixed on the top of each of the sliders, and a tube rack, a vortex mixer and a centrifuge are respectively placed on the top of the three placement tables.

[0013] Preferably, a controller is installed on the front surface of the rectangular plate, an L-shaped plate is fixed on the other side of the workbench, two symmetrical auxiliary frames are installed on the other side of the workbench, the tops of the two auxiliary frames are in contact with the bottom of the L-shaped plate, a photometer is arranged on the upper side of the L-shaped plate, and each of the motor, two electric valves, flow sensor, first electric push rod and centrifugal device are electrically connected to the controller.

[0014] Preferably, a concave plate is fixed to one side of the interior of the workbench, a collection box is arranged inside the concave plate, a second electric push rod is installed on the lower side of the workbench, the second electric push rod is electrically connected to the controller, and a box cover is installed at the bottom of the telescopic end of the second electric push rod.

[0015] Preferably, a sealing ring is bonded to the bottom of the box cover, a disinfection lamp is installed at the bottom of the box cover, and the wire of the disinfection lamp movably passes through the bottom of the box cover, the disinfection lamp is electrically connected to the controller, and a placement groove is opened on the top of the workbench.

[0016] Compared with the prior art, the present invention has the following beneficial effects: 1. The present invention can recycle and reuse substances in some solvents used in the process of sample nucleic acid extraction, purification and detection by setting an auxiliary mechanism, thereby improving the utilization rate of resources, that is, improving the utilization efficiency of the integrated instrument for nucleic acid extraction, detection and analysis. When it is necessary to treat the waste liquid generated after the sample liquid is mixed with the lysis buffer and centrifuged and filtered, the controller, the first electric push rod, the hollow block, the funnel-shaped tube filled with the phenol-chloroform solution, the connecting tube, the electric valve, the flow sensor and the first circular tube are used to inject an accurate volume of the phenol-chloroform solution into the test tube, and then the prepared centrifuge tube and the centrifugal device are used to centrifuge the mixed waste liquid and the phenol-chloroform solution into organic phase and aqueous phase layers.

[0017] 2. The present invention can achieve the goal of fully mixing the remaining organic phase solution and the isopropanol solution into an organic phase and an aqueous phase by using the cooperation of a controller, a first electric push rod, a hollow block, a funnel-shaped tube filled with an isopropanol solution, a connecting tube, an electric valve, a flow sensor and a first round tube. Then, with the cooperation of a hand-tightened bolt, a mounting groove, a T-block, an asbestos net and a placement ring, the top of the evaporating dish can be tightly contacted with the bottom of the second round tube to prevent heat from radiating into the interior of the tank shell. Afterwards, with the cooperation of an alcohol lamp, an asbestos net and an evaporating dish, the solvent in the organic phase can be evaporated to obtain proteinase K that can be recycled and reused.

[0018] 3. The present invention can perform nucleic acid extraction, purification, detection and analysis operations on samples by setting up an integrated processing mechanism, so as to obtain the desired results. When nucleic acid extraction is required for the sample liquid, the motor, rectangular groove, placement rack, threaded rod, controller, slide, slider and placement table are first used to cooperate with the liquid storage tube, tube rack, vortex mixer and centrifuge to move the required equipment to the front of the detection personnel standing on the front surface of the workbench, and then the tube rack, microcentrifuge tube and lysis buffer are used to separate the viral RNA molecules in the sample.

[0019] 4. The present invention can combine RNA molecules with silica gel membranes by using the cooperation of centrifugal columns, stationary phases and centrifuges, and then remove the residual impurities on the silica gel membrane by using the cooperation of centrifugal columns, centrifuges and washing liquids. After that, the purified RNA samples can be obtained by using the cooperation of centrifugal columns, elution buffers and centrifuges, and then the RNA samples can be detected and analyzed by using a photometer.

[0020] 5. The present invention can seal the top opening of the collection box by the cooperation of the second electric push rod, the controller, the box cover and the sealing ring, and then disinfect the waste liquid collected in the collection box under the action of the disinfection lamp. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 This is a stereogram of an integrated instrument for nucleic acid extraction, detection and analysis according to the present invention; Figure 2 This is a partial three-dimensional diagram of the auxiliary mechanism of an integrated instrument for nucleic acid extraction, detection and analysis of the present invention; Figure 3 This is a top-down perspective view of an integrated instrument for nucleic acid extraction, detection and analysis according to the present invention; Figure 4 This is a schematic diagram of the three-dimensional structure of a threaded rod, a sliding block and a placement table of an integrated instrument for nucleic acid extraction, detection and analysis of the present invention; Figure 5This is a schematic diagram of the three-dimensional structure of a placement rack and a liquid storage tube of an integrated instrument for nucleic acid extraction, detection and analysis of the present invention; Figure 6 This is a schematic diagram of the three-dimensional structure of a placement ring and a T-block of an integrated instrument for nucleic acid extraction, detection and analysis of the present invention; Figure 7 A three-dimensional diagram of a concave plate of an integrated instrument for nucleic acid extraction, detection and analysis according to the present invention; Figure 8 This is a three-dimensional diagram of a limit block of an integrated instrument for nucleic acid extraction, detection and analysis according to the present invention; Fig. 9 This is a schematic diagram of the three-dimensional structure of a second electric rod, a box cover, a sealing ring and a disinfection lamp of an integrated instrument for nucleic acid extraction, detection and analysis of the present invention.

[0022] In the figure: 1. integrated processing mechanism; 101. workbench; 102. support column; 103. rectangular groove; 104. slideway; 105. threaded rod; 106. motor; 107. placement rack; 108. liquid storage tube; 109. tube cover; 110. slider; 111. placement table; 112. tube rack; 113. vortex mixer; 114. centrifuge; 115. rectangular plate; 116. controller; 117. L-shaped plate; 118. auxiliary rack; 119. photometer; 2. auxiliary mechanism; 201. shell; 202. shell cover; 203. limit block; 204. mounting rack; 205. funnel tube; 2 06. Pipe plug; 207. Connecting pipe; 208. Electric valve; 209. Flow sensor; 210. First round tube; 211. First electric push rod; 212. Hollow block; 213. Test tube; 214. Rectangular block; 215. Second round tube; 216. Mounting groove; 217. Placement ring; 218. T-block; 219. Asbestos mesh; 220. Evaporating dish; 221. Limiting ring; 222. Tightening bolt; 223. Placement plate; 224. Alcohol lamp; 225. Centrifugal device; 3. Concave plate; 4. Collecting box; 5. Second electric push rod; 6. Box cover; 7. Sealing ring; 8. Disinfection lamp; 9. Placement groove. DETAILED DESCRIPTION

[0023] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the implementation regulations described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0024] See also Figure 1-Figure 9 As shown, the present invention provides a technical solution: an integrated instrument for nucleic acid extraction, detection and analysis, comprising an integrated processing mechanism 1, and an auxiliary mechanism 2 is provided on one side of the integrated processing mechanism 1; The auxiliary mechanism 2 includes a shell 201, and a wire outlet hole is set on the shell 201. A mounting frame 204 is installed on the top of the shell 201. Funnel-shaped tubes 205 are fixed inside the two through holes of the mounting frame 204. Connecting tubes 207 are installed at the output ends of the two funnel-shaped tubes 205. Electric valves 208 are installed at the output ends of the two connecting tubes 207. Flow sensors 209 are installed at the output ends of the two electric valves 208. First round tubes 210 are installed at the output ends of the two flow sensors 209. A mounting groove 216 is opened on the front surface of the inner wall of the shell 201. A first electric push rod 211 is installed on the rear surface of the inner wall of the shell 201. The first electric push rod 211 A hollow block 212 is fixed to the telescopic end, a test tube 213 is arranged inside the hollow block 212, a rectangular block 214 is fixed to the top of the inner wall of the shell 201, a T-block 218 is slidably connected inside the mounting groove 216, a placement ring 217 is fixed to the side of the T-block 218 close to the rectangular block 214, an asbestos net 219 is arranged on the top of the placement ring 217, an evaporating dish 220 is arranged on the top of the asbestos net 219, a hand-tightening bolt 222 is threaded through the front surface of the shell 201, a placement plate 223 is fixed to the front surface of the inner wall of the shell 201, an alcohol lamp 224 is arranged at the top groove of the placement plate 223, and a centrifugal device 225 is arranged on the top of the inner wall of the shell 201.

[0025] according to Figure 1-Figure 3 , Figure 6 and Figure 8 As shown, the shell 201 is located between the two limit blocks 203, the output ends of the two funnel-shaped tubes 205 are movable through the top of the shell 201, and the two flow sensors 209 are respectively installed on the front surface and the rear surface of the rectangular block 214. One end of the hand-tightening bolt 222 is threadedly connected to the front surface of the placement ring 217, so that the placement ring 217 can be fixed after moving with the cooperation of the hand-tightening bolt 222 and the T-block 218.

[0026] according to Figure 1-Figure 3 and Figure 8 As shown, a shell cover 202 is provided on one side of the shell 201, and limiting blocks 203 are fixed on the front surface and the rear surface of the shell 201. Pipe plugs 206 are installed on the tops of the two funnel-shaped tubes 205. A second round tube 215 is fixedly penetrated through the top of the shell 201. A limiting ring 221 is movably sleeved on the outer wall of the evaporating dish 220, and the limiting ring 221 is fixed on the front surface of the inner wall of the shell 201. Under the action of the limiting ring 221, the evaporating dish 220 can be prevented from being more stable and not moving during operation.

[0027] according to Figure 1-Figure 3As shown, the integrated processing mechanism 1 includes a workbench 101, a shell 201 is fixed on one side of the workbench 101, a plurality of support columns 102 are fixed on the bottom of the workbench 101, four rectangular grooves 103 are opened on the inner wall of the workbench 101, and three slide grooves 104 are evenly distributed on the bottom of the inner wall of the workbench 101. With the cooperation of the slide grooves 104 and the slider 110, the placement table 111 can be moved horizontally.

[0028] according to Figure 1 , Figure 3 and Figure 4 As shown, the inner wall front surface of each rectangular groove 103 and the inner wall front surface of each slide groove 104 are rotatably connected with a threaded rod 105 through a bearing, a rectangular plate 115 is fixed on one side of the workbench 101 near the edge of the front surface, and seven motors 106 are installed on the rear surface of the workbench 101, so that the motor 106 can provide power support for the movement of the placement rack 107 and the placement table 111.

[0029] according to Figure 1 and Figure 3-Figure 5 As shown, one end of four threaded rods 105 close to the motor 106 respectively moves through the rear surface of the inner wall of the four rectangular grooves 103, and one end of the other three threaded rods 105 close to the motor 106 respectively moves through the rear surface of the inner wall of the three slide grooves 104, and one end of each threaded rod 105 close to the motor 106 is installed with the output end of each motor 106 respectively, and the interior of each rectangular groove 103 is slidably connected with a placement rack 107, which can drive the liquid storage tube 108 to move back and forth horizontally with the cooperation of the placement rack 107, the rectangular groove 103, the threaded rod 105 and the motor 106.

[0030] according to Figure 1 and Figure 3-Figure 5 As shown, one end of four threaded rods 105 are respectively threaded through the side of four placement racks 107 close to the motor 106, each placement rack 107 is provided with a liquid storage tube 108 inside, and a tube cover 109 is provided on the top of each liquid storage tube 108, and a slider 110 is slidably connected to the inside of each slide groove 104, and one end of the other three threaded rods 105 are respectively threaded through the side of three sliders 110 close to the motor 106, and a placement table 111 is fixed on the top of each slider 110, and a tube rack 112, a vortex mixer 113 and a centrifuge 114 are respectively placed on the top of the three placement tables 111, so that the lysis buffer and the sample liquid can be fully mixed together under the action of the vortex mixer 113.

[0031] according to Figure 1-Figure 3As shown, a controller 116 is installed on the front surface of the rectangular plate 115, an L-shaped plate 117 is fixed on the other side of the workbench 101, and two symmetrical auxiliary frames 118 are installed on the other side of the workbench 101. The tops of the two auxiliary frames 118 are in contact with the bottom of the L-shaped plate 117, and a photometer 119 is arranged on the upper side of the L-shaped plate 117. Each motor 106, two electric valves 208, a flow sensor 209, and a first electric push rod 211 are electrically connected to the controller 116. Under the action of the controller 116, the components electrically connected to the controller 116 can be controlled to perform opening and closing operations.

[0032] according to Figure 1 , Figure 3 , Figure 7 and Fig. 9 As shown, a concave plate 3 is fixed to one side of the interior of the workbench 101, a collecting box 4 is arranged inside the concave plate 3, a second electric push rod 5 is installed on the lower side of the workbench 101, the second electric push rod 5 is electrically connected to the controller 116, and a box cover 6 is installed at the bottom of the telescopic end of the second electric push rod 5, so that the disinfection lamp 8 can perform ultraviolet disinfection operation on the waste liquid in the collecting box 4 with the cooperation of the box cover 6 and the sealing ring 7.

[0033] according to Figure 1 , Figure 3 and Fig. 9 As shown, a sealing ring 7 is bonded to the bottom of the box cover 6, a disinfection lamp 8 is installed at the bottom of the box cover 6, and the wire of the disinfection lamp 8 runs through the bottom of the box cover 6, the disinfection lamp 8 is electrically connected to the controller 116, and a placement groove 9 is provided on the top of the workbench 101, and the sample tubes that need to be subjected to nucleic acid extraction, purification and detection can be placed under the action of the placement groove 9.

[0034] The effect achieved by the entire mechanism is: when it is necessary to perform biological detection on the sample, the controller 116 is first connected to the external power supply, and after the external power supply is connected, the controller 116 is turned on, and the flow threshold and the use program are set, and then the vortex mixer 113, the centrifuge 114, the centrifugal device 225 and the photometer 119 are all connected to the external power supply, and then all the tube covers 109 are removed, and through Figure 1As shown, from left to right, an appropriate amount of lysis buffer, washing solution, elution buffer and clean water are sequentially injected into the liquid storage tube 108. After the liquid injection operation is completed in each liquid storage tube 108, all the tube covers 109 are installed back to the initial position, and then the two tube plugs 206 are removed, and then an appropriate amount of phenol chloroform solution and isopropanol solution are directly injected into the two funnel-shaped tubes 205, respectively, and then the two tube plugs 206 are respectively installed back to the initial position. When everything is ready, the sample tube containing the sample solution is first placed in the placement groove 9, and then the controller 116 is used to start the corresponding tube rack 112 and the motor 106 that drives the liquid storage tube 108 containing the lysis buffer to move. At this time, the two started motors 106 will move at the corresponding With the cooperation of the slide 104, the slider 110, the rectangular groove 103 and the threaded rod 105, the corresponding placement table 111 and the placement rack 107 are directly driven to move, and the moving placement table 111 and the placement rack 107 will directly drive the tube rack 112 in contact with them and the liquid storage tube 108 inside the placement rack 107 to move. When the placement table 111 and the placement rack 107 are moved to a state where they cannot move, the controller 116 will turn off the corresponding motor 106 respectively. At this time, the tube rack 112 and the liquid storage tube 108 filled with lysis buffer are just in front of the inspector standing on the front surface of the workbench 101. At this time, the inspector can take out the prepared microcentrifuge tube and place it in the groove on the tube rack 112, and then take the sample tube out The interior of the placement slot 9 is taken out and opened, and then the sample solution in the sample tube and the lysis buffer in the storage tube 108 are injected into the microcentrifuge tube in sequence using a tool. When the lysis buffer contacts the sample solution, the lysis buffer (usually composed of phenol, proteinase K and guanidine isothiocyanate, etc.) can directly denature the sample solution to a high degree. At the same time, the lysis buffer also contains some RNase inhibitors, which can ensure that the viral RNA is completely separated. Then, the controller 116 is used to synchronously start the motor 106 that drives the storage tube 108 filled with washing solution to move, the motor 106 that drives the storage tube 108 filled with elution buffer solution to move, and the motor 106 corresponding to the vortex mixer 113. At this time, the started motor 106 will move in the corresponding slide slot 1 04. With the cooperation of the slider 110, the rectangular groove 103 and the threaded rod 105, the corresponding placement table 111 and the two placement racks 107 are directly driven to move, and the moving placement table 111 and the two placement racks 107 will directly drive the tube rack 112 in contact with them and the liquid storage tubes 108 respectively located inside the two placement racks 107 to move. When the placement table 111 and the two placement racks 107 are moved to the point where they cannot move, the controller 116 will turn off the corresponding motors 106 respectively. At this time, the tube rack 112, the liquid storage tube 108 filled with lysis buffer and the liquid storage tube 108 filled with elution buffer are just in front of the inspector standing on the front surface of the workbench 101. At this time, the tube mouth of the microcentrifuge tube is directly installed with a cover.Then, the microcentrifuge tube is taken down from the tube rack 112, and then the microcentrifuge tube containing the lysate and the sample solution is placed in the vortex mixer 113, and then the vortex mixer 113 is directly started. At this time, the started vortex mixer 113 allows the two liquids to be fully mixed. When the two solutions have completed the full mixing operation, the vortex mixer 113 is turned off, and then the microcentrifuge tube is taken out from the vortex mixer 113, and then placed on the tube rack 112. Then, the vortex mixer 113 is reset and moved back to the initial position by using the cooperation of the controller 116 and the motor 106 corresponding to the vortex mixer 113, and then the cover on the processed microcentrifuge tube on the tube rack 112 is opened, and the centrifuge column is taken out, and then the microcentrifuge tube is placed on the tube rack 112. The liquid in the centrifuge tube is extracted and injected into the centrifuge column, and the stationary phase composed of a silica gel matrix is ​​placed in it. Finally, the cover on the centrifuge column is installed. When the solution in the centrifuge column needs to be centrifuged, the controller 116 is used to start the motor 106 corresponding to the centrifuge 114. At this time, the started motor 106 will directly move the centrifuge 114 on the top of the placement table 111 to the front of the tester with the cooperation of the corresponding slide 104, threaded rod 105, slider 110 and placement table 111. Then, the centrifuge column containing the sample is placed in the centrifuge 114, and then the centrifuge 114 is directly started. At this time, the started centrifuge 114 will directly centrifuge the mixture in the centrifuge column, and during the centrifugation process, the centrifuge 114 will be directly centrifuged. During the process, RNA molecules will directly bind to the silica gel membrane. When the mixture in the centrifugal column completes the centrifugal operation, the centrifuge 114 is turned off first, and then the treated centrifugal column is removed from the centrifuge 114. Then the centrifugal column cover is opened to filter out the liquid in the centrifugal column. Then the washing liquid in the liquid storage tube 108 is extracted by a tool and injected into the interior of the centrifugal column. Then the centrifugal column cover is installed again, and then the centrifugal operation is performed again by the centrifuge 114. When the centrifugal column is centrifuged again, the washing liquid in the centrifugal column will pass through the silica gel membrane to remove the impurities remaining on the silica gel membrane. When the centrifugal column completes the centrifugal operation, only the RNA bound to it is left on the silica gel membrane. Then the centrifugal column cover is removed to discharge the liquid in the centrifugal column. The elution solution in the liquid storage tube 108 is then drawn out and injected into the centrifugal column, and the lid is installed again after the liquid is injected. Then, the centrifuge 114 is used for centrifugal operation again. At this time, the elution buffer will pass through the silica gel membrane under the action of centrifugation, thereby removing the viral RNA adsorbed on the membrane from the centrifugal column. Then, the lid of the centrifugal column after the final treatment is opened, and the liquid in the centrifugal column is discharged to obtain the purified nucleic acid RNA sample. Then, the controller 116, the motor 106 corresponding to the tube rack 112 and the motor 106 corresponding to the centrifuge 114 are used to reset the tube rack 112 and the centrifuge 114 to their initial positions, and finally, the purified nucleic acid RNA sample is removed from the centrifugal column.Move it to the detection box on the photometer 119, and start the photometer 119 to detect the substance in the detection box after completing the moving operation. At the same time, after completing the lysis buffer mixture centrifugal filtration, directly inject the obtained waste liquid into the test tube 213. After the entire sample nucleic acid extraction, detection and analysis are processed, directly put the test tube 213 containing the waste liquid into the interior of the hollow block 212, and then use the controller 116 to start the first electric push rod 211, so that the started first electric push rod 211 drives the test tube 213 placed on the hollow block 212 to move to the bottom position of the output end of the first circular tube 210 close to the hollow block 212. When the test tube 213 moves to the bottom position of the output end of the first circular tube 210 close to the hollow block 212, the controller 116 is used to start the first electric push rod 211. The controller 116 will directly close the first electric push rod 211, and then start the electric valve 208 corresponding to the first round tube 210 near the hollow block 212. When the electric valve 208 is opened, the phenol-chloroform solution in the corresponding funnel-shaped tube 205 will directly flow into the connecting tube 207 connected thereto, and then be transported to the inside of the opened electric valve 208, and then pass through the inside of the corresponding flow sensor 209, and then be transported to the corresponding first round tube 210, and then be guided to the inside of the test tube 213. At the same time, when the solution passes through the inside of the flow sensor 209, the flow sensor 209 will continuously count the flow rate and continuously transmit the obtained data to the inside of the controller 116 in the form of electrical signals. When the controller When the controller 116 receives data from the flow sensor 209, the controller 116 will directly compare the received data with the flow threshold value set in advance by the controller 116. When the data received by the controller 116 is lower than the flow threshold value set in advance by the controller 116, the controller 116 will not close the opened electric valve 208. When the data received by the controller 116 reaches the flow threshold value set in advance by the controller 116, the controller 116 will directly close the opened electric valve 208, stop the first round tube 210 from injecting the solution into the test tube 213, and then directly pour the two liquids in the test tube 213 into the centrifuge tube, then install the lid, shake and mix evenly, and then put it into the centrifuge device 22. 5, and then the centrifugal device 225 is started to centrifuge the mixture in the centrifuge tube. When the centrifugal tube completes the centrifugal operation, the centrifugal device 225 is closed, and then the centrifuge tube is taken out from the inside of the centrifuge device 225. At this time, the liquid inside the centrifuge tube after being taken out is an upper and lower layer, namely an organic phase and an aqueous phase. Then the organic phase is removed from the centrifuge tube and moved to a new centrifuge tube. After that, the centrifuge tube containing the organic phase is placed in the hollow block 212. Then, the controller 116, the hollow block 212 and the first electric push rod 211 are used to move the centrifuge tube to the bottom position of the output end of another first circular tube 210. When the centrifuge tube moves to the position of another first circular tube 210, the controller 116 will directly close the first electric push rod 211.Then, the electric valve 208 corresponding to the other first circular tube 210 is directly started. When the electric valve 208 is opened, the isopropyl alcohol solution in the corresponding funnel-shaped tube 205 will move directly to the connecting tube 207 connected thereto, and then enter the interior of the opened electric valve 208, and then enter the corresponding flow sensor 209, and then be transported to the interior of the first circular tube 210 connected thereto, and then be directed to the centrifuge tube to mix with the treated liquid. At the same time, when the solution passes through the interior of the flow sensor 209, the flow sensor 209 will continuously count the flow rate, and continuously transmit the obtained data to the interior of the controller 116 in the form of electrical signals. When the controller 116 receives the flow sensor 2 09, the controller 116 will directly compare the received data with the flow threshold value set in advance by the controller 116. When the data received by the controller 116 is lower than the flow threshold value set in advance by the controller 116, the controller 116 will not close the opened electric valve 208. When the data received by the controller 116 reaches the flow threshold value set in advance by the controller 116, the controller 116 will directly close the opened electric valve 208, stop the first circular tube 210 from injecting the solution into the interior of the centrifuge tube, and when the interior of the centrifuge tube is completely injected with the isopropanol solution, directly use the cover to cover the outlet of the centrifuge tube, then remove the centrifuge tube from the interior of the hollow block 212, and then use the centrifugal device 225 to centrifuge again. The centrifugal tube is centrifuged again. When the centrifugal tube completes the centrifugal operation, the centrifugal device 225 is directly closed at this time, and then the centrifugal tube is taken out from the inside of the centrifugal device 225. At this time, the liquid inside the centrifugal tube after being taken out is two layers, namely, the organic phase and the aqueous phase. Then the organic phase is removed from the centrifugal tube and poured into the evaporating dish 220. Then the evaporating dish 220 is placed on the asbestos net 219. When the evaporating dish 220 completes the placement operation, the placement ring 217 is directly moved upward. At this time, the moving placement ring 217 will directly drive the evaporating dish 220 to move upward with the cooperation of the mounting groove 216, the T-block 218 and the asbestos net 219. When the top of the evaporating dish 220 passes through the inside of the limiting ring 221 and contacts with the bottom of the second circular tube 215, it is directly moved upward at this time. Twist the hand screw bolt 222 until one end of the hand screw bolt 222 is threadedly connected to the T-block 218 to fix the placement ring 217, and then light the alcohol lamp 224 to evaporate the liquid in the evaporating dish 220. When the solvent in the liquid organic phase is evaporated, turn off the alcohol lamp 224. At this time, the liquid left in the evaporating dish 220 can be proteinase K. Then, remove the evaporating dish 220, and pour out the liquid in the evaporating dish 220 to obtain the substance in the used solvent, thereby avoiding the waste of resources and improving the use efficiency of the integrated instrument for nucleic acid extraction, detection and analysis. At the same time, other waste liquid generated after the sample nucleic acid is extracted and purified will also be directly poured into the collection box 4. At the same time, after each time the waste liquid is poured into the collection box 4,Directly use the cooperation of the controller 116, the second electric push rod 5, the box cover 6 and the sealing ring 7 to seal the top opening of the collection box 4. After the sample nucleic acid extraction, purification, detection and analysis are completed, directly use the controller 116 to turn on the disinfection lamp 8, and let the activated disinfection lamp 8 disinfect the waste liquid in the collection box 4. When the waste liquid is disinfected, directly use the controller 116 to turn off the disinfection lamp 8, and then use the cooperation of the controller 116 and the second electric push rod 5 to reset the box cover 6 back to the initial position, and then the disinfected waste liquid can be processed later.

[0035] Among them, the motor 106, the vortex mixer 113, the centrifuge 114 (centrifugal equipment 225), the controller 116 (PLC controller), the photometer 119 (UV-visible spectrophotometer), the electric valve 208, the flow sensor 209, the first electric push rod 211, the second electric push rod 5 and the disinfection lamp 8 are all existing technologies and will not be explained in detail here.

[0036] Although the present invention has been described in detail with reference to the aforementioned embodiments, it is still possible for those skilled in the art to modify the technical solutions described in the aforementioned embodiments, or to make equivalent substitutions for some of the technical features therein. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. An integrated instrument for nucleic acid extraction, detection and analysis, characterized in that: It comprises an integrated processing mechanism (1), wherein an auxiliary mechanism (2) is provided on one side of the integrated processing mechanism (1); The auxiliary mechanism (2) comprises a shell (201), a mounting frame (204) is installed on the top of the shell (201), a funnel-shaped tube (205) is fixed inside two through holes of the mounting frame (204), a connecting tube (207) is installed at the output ends of the two funnel-shaped tubes (205), an electric valve (208) is installed at the output ends of the two connecting tubes (207), a flow sensor (209) is installed at the output ends of the two electric valves (208), a first round tube (210) is installed at the output ends of the two flow sensors (209), a mounting groove (216) is provided on the front surface of the inner wall of the shell (201), a first electric push rod (211) is installed on the rear surface of the inner wall of the shell (201), and a hollow block (210) is fixed to the telescopic end of the first electric push rod (211) 212), a test tube (213) is arranged inside the hollow block (212), a rectangular block (214) is fixed on the top of the inner wall of the shell (201), a T-shaped block (218) is slidably connected inside the mounting groove (216), a placement ring (217) is fixed on the side of the T-shaped block (218) close to the rectangular block (214), an asbestos net (219) is arranged on the top of the placement ring (217), an evaporating dish (220) is arranged on the top of the asbestos net (219), a hand-tightening bolt (222) is threadedly penetrated on the front surface of the shell (201), a placement plate (223) is fixed on the front surface of the inner wall of the shell (201), an alcohol lamp (224) is arranged at the groove on the top of the placement plate (223), and a centrifugal device (225) is arranged on the top of the inner wall of the shell (201).

2. The integrated instrument for nucleic acid extraction, detection and analysis according to claim 1, characterized in that: The housing (201) is located between the two limit blocks (203), the output ends of the two funnel-shaped tubes (205) are movable through the top of the housing (201), the two flow sensors (209) are respectively mounted on the front surface and the rear surface of the rectangular block (214), and one end of the hand-tightening bolt (222) is threadedly connected to the front surface of the placement ring (217).

3. The integrated instrument for nucleic acid extraction, detection and analysis according to claim 1, characterized in that: A shell cover (202) is provided on one side of the shell (201); a limiting block (203) is fixed to the front surface and the rear surface of the shell (201); a pipe plug (206) is installed on the top of each of the two funnel-shaped tubes (205); a second round tube (215) is fixedly passed through the top of the shell (201); a limiting ring (221) is movably sleeved on the outer wall of the evaporating dish (220); and the limiting ring (221) is fixed to the front surface of the inner wall of the shell (201).

4. The integrated instrument for nucleic acid extraction, detection and analysis according to claim 1, characterized in that: The integrated processing mechanism (1) comprises a workbench (101), the shell (201) is fixed on one side of the workbench (101), a plurality of support columns (102) are fixed on the bottom of the workbench (101), four rectangular grooves (103) are provided on the inner wall of the workbench (101), and three slide grooves (104) are equidistantly distributed on the bottom of the inner wall of the workbench (101).

5. The integrated instrument for nucleic acid extraction, detection and analysis according to claim 4, characterized in that: The inner wall front surface of each rectangular groove (103) and the inner wall front surface of each slide groove (104) are rotatably connected to a threaded rod (105) via a bearing, a rectangular plate (115) is fixed to one side of the workbench (101) near the edge of the front surface, and seven motors (106) are installed on the rear surface of the workbench (101).

6. The integrated instrument for nucleic acid extraction, detection and analysis according to claim 5, characterized in that: One end of four of the threaded rods (105) close to the motor (106) movably penetrates the rear surfaces of the inner walls of the four rectangular slots (103), and one end of the other three threaded rods (105) close to the motor (106) movably penetrates the rear surfaces of the inner walls of the three slide slots (104), and one end of each threaded rod (105) close to the motor (106) is respectively installed with the output end of each motor (106), and a placement rack (107) is slidably connected inside each rectangular slot (103).

7. The integrated instrument for nucleic acid extraction, detection and analysis according to claim 6, characterized in that: One end of four of the threaded rods (105) are respectively threadedly penetrated through one side of four placement racks (107) close to the motor (106); a liquid storage tube (108) is provided inside each of the placement racks (107); a tube cover (109) is provided on the top of each of the liquid storage tubes (108); a slider (110) is slidably connected inside each of the slide grooves (104); one end of the other three threaded rods (105) are respectively threadedly penetrated through one side of three sliders (110) close to the motor (106); a placement table (111) is fixed on the top of each of the sliders (110); and a tube rack (112), a vortex mixer (113) and a centrifuge (114) are respectively placed on the top of the three placement tables (111).

8. The integrated instrument for nucleic acid extraction, detection and analysis according to claim 5, characterized in that: A controller (116) is installed on the front surface of the rectangular plate (115); an L-shaped plate (117) is fixed on the other side of the workbench (101); two symmetrical auxiliary frames (118) are installed on the other side of the workbench (101); the tops of the two auxiliary frames (118) are in contact with the bottom of the L-shaped plate (117); a photometer (119) is arranged on the upper side of the L-shaped plate (117); and each of the motors (106), the two electric valves (208), the flow sensor (209), and the first electric push rod (211) are electrically connected to the controller (116).

9. The integrated instrument for nucleic acid extraction, detection and analysis according to claim 1, characterized in that: A concave plate (3) is fixed to one side of the interior of the workbench (101), a collection box (4) is arranged inside the concave plate (3), a second electric push rod (5) is installed on the lower side of the workbench (101), the second electric push rod (5) is electrically connected to the controller (116), and a box cover (6) is installed at the bottom end of the telescopic end of the second electric push rod (5).

10. The integrated instrument for nucleic acid extraction, detection and analysis according to claim 9, characterized in that: A sealing ring (7) is bonded to the bottom of the box cover (6), a disinfection lamp (8) is installed at the bottom of the box cover (6), and a wire of the disinfection lamp (8) movably passes through the bottom of the box cover (6), the disinfection lamp (8) is electrically connected to the controller (116), and a placement groove (9) is provided on the top of the workbench (101).

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