PCR (Polymerase Chain Reaction) eight-row tube with high practicability
By introducing a temperature detection head and a one-way exhaust mechanism into the PCR eight-shift pipe, the reagent contamination problems caused by insufficient temperature monitoring and gas expansion in the prior art are solved, and more accurate experimental results and lower pollution risks are achieved.
Patent Information
- Application Number
- CN202510273082.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-03-10
- Publication Date
- 2025-06-06
- Estimated Expiration
- Not applicable · inactive patent
AI Technical Summary
The existing PCR eight-shift tube lacks a temperature monitoring system for each PCR tube, resulting in inaccurate experimental results; at the same time, the tube cover is prone to open due to gas expansion in high-temperature experiments, resulting in reagent contamination.
A PCR eight-track tube including eight integrated pipe bodies is designed, each pipe body is equipped with a temperature detection head and a one-way air outlet mechanism. The temperature detection head is used to detect and transmit temperature and data through the temperature measuring frame and processor module; the one-way air outlet mechanism realizes one-way exhaust through sealing balls and guiding slopes to prevent external gas from entering.
Accurate temperature monitoring of each PCR tube is achieved, improving the accuracy of experimental results; at the same time, the one-way air vent mechanism is used to avoid opening the tube cover caused by gas expansion, reducing the risk of reagent contamination.
Smart Images
Figure CN120098759A_ABST
Abstract
Description
Technical Field
[0001] The present invention relates to the technical field related to detection instruments, and in particular to a highly practical PCR eight-tube strip. Background Art
[0002] Reagents, also known as biochemical reagents or test drugs, are mainly pure chemicals used to achieve chemical reactions, analytical tests, research experiments, teaching experiments, and chemical formulas. They are generally divided into general reagents, high-purity reagents, analytical reagents, instrumental analytical reagents, clinical diagnostic reagents, biochemical reagents, inorganic ion colorimetric reagents, etc. Eight-row PCR tubes are the most commonly used reaction tubes for fluorescent quantitative PCR tests, and fluorescent quantitative PCR tests are currently widely used in the fields of biology and medicine. The existing eight-row PCR tubes lack a temperature monitoring system for each PCR tube. When the PCR tubes are in different positions in the PCR instrument, the temperature is also different, which leads to inaccurate experimental results. In addition, the existing PCR tubes are prone to opening the tube caps due to gas expansion during high-temperature experiments, which can easily cause reagent contamination and inaccurate experimental results. Summary of the invention
[0003] In order to solve the defects in the prior art, the present invention provides a highly practical PCR eight-tube strip.
[0004] In order to solve the above technical problems, the present invention provides the following technical solutions:
[0005] The present invention discloses a highly practical PCR eight-row tube, comprising eight tube bodies connected as one, wherein the upper ends of the tube bodies are connected as one through a connecting plate, the upper end of each tube body is connected to a tube cover through a flexible sheet, and the tube cover is provided with a plug inserted into the upper port of the tube body, and the front end of the plug is provided with a guide portion, the plug is interference-fitted with the upper end port of the tube body, the tube cover is provided with an exhaust pipe, and the exhaust pipe is provided with a one-way exhaust mechanism;
[0006] The temperature measuring frame is also included for measuring the temperature inside each tube body, and the temperature measuring frame includes a ring which is sleeved on the outside of the tube body and lifts the tube body, each ring is connected to form an integral body through a connecting frame, and the temperature measuring frame is provided with Positioning shell , and the positioning housing is provided with a processor module, and the temperature measuring rack is provided with eight temperature collection interfaces connected to the processor module;
[0007] Each of the tube bodies is provided with a connecting tube connected to the interior of the tube body, and each of the connecting tubes has a temperature detection head installed on the disassembly mechanism; the processor module is provided with a communication module, and the processor module transmits the temperature data detected by each temperature detection probe to the monitoring terminal through the communication module.
[0008] As a preferred technical solution of the present invention, the one-way air outlet mechanism includes a lower connecting block and an upper connecting block, and the lower connecting block is provided with a first groove, and the upper connecting block is provided with a second groove, and the first groove and the second groove are connected to form a cavity, a sealing ball is provided in the cavity, and the lower connecting block is provided with a first channel on the side of the first groove, and the bottom of the first groove is provided with a guide slope inclined toward the first channel, the upper connecting block is provided with a second channel, and the upper connecting block and the lower connecting block are threadedly connected.
[0009] As a preferred technical solution of the present invention, the lower connecting block is provided with a butt joint connected to the exhaust pipe, and the butt joint is threadedly connected to the exhaust pipe, and the size of the cavity is slightly larger than the size of the sealing ball.
[0010] As a preferred technical solution of the present invention, the sealing ball is a steel ball.
[0011] As a preferred technical solution of the present invention, the temperature detection head includes a temperature detection probe, and the outside of the temperature detection probe is provided with an insulating block that wraps the outer end of the temperature detection probe, the temperature detection probe is connected to a wire extending from the insulating block, and the outer end of the wire is provided with a plug inserted into the temperature acquisition interface; the disassembly mechanism includes an external thread structure provided on the insulating block, and the insulating block is threadedly connected to the connecting pipe.
[0012] As a preferred technical solution of the present invention, the connecting pipe is arranged at the bottom of the pipe body.
[0013] As a preferred technical solution of the present invention, the working method of the PCR eight-row tube is as follows: first, the temperature detection head and the one-way air outlet mechanism are disinfected. After the disinfection is completed, the temperature detection head is installed on the connecting pipe at the bottom of the tube body, and the one-way air outlet mechanism is installed on the exhaust pipe. Then, the reagent is loaded on the tube body, and the tube cover is covered. The tube body is placed on a temperature measuring rack, and the plugs of each temperature detection head are inserted into the temperature collection interface. When in use, the temperature detection head detects and collects the reagent temperature in each tube body. When the one-way air outlet mechanism generates gas expansion in the tube body, the gas passes through the first top and rolls the dynamic sealing ball to one side, thereby connecting the first channel and the second channel, thereby exhausting the gas. When the air pressure in the tube body drops, the sealing ball moves back and forth under the action of the guide slope, thereby blocking the first channel and preventing external gas from entering.
[0014] The beneficial effects of the present invention are:
[0015] 1. This highly practical PCR eight-row tube first disinfects the temperature detection head and the one-way air outlet mechanism. After the disinfection is completed, the temperature detection head is installed on the connecting pipe at the bottom of the tube body, and the one-way air outlet mechanism is installed on the exhaust pipe. Then, the reagent is installed on the tube body, the tube cover is covered, the tube body is placed on the temperature measuring rack, and the plugs of each temperature detection head are inserted into the temperature collection interface. When in use, the temperature detection head detects and collects the reagent temperature in each tube body. When the one-way air outlet mechanism generates gas expansion in the tube body, the gas passes through the first top and rolls the dynamic sealing ball to one side, so that the first channel and the second channel are connected, so as to exhaust. When the air pressure in the tube body drops, the sealing ball moves back and forth under the action of the guide slope, thus blocking the first channel to prevent external gas from entering, thereby reducing the influence of temperature and making the experimental data more accurate.
[0016] 2. This highly practical PCR eight-row tube is provided with a specific one-way air outlet mechanism, and the upper connecting block and the lower connecting block are threadedly connected, and has the characteristics of simple structure and easy disassembly and disinfection. When gas expansion occurs in the tube body, the gas passes through the first upper top, and the dynamic sealing ball rolls to one side, so that the first channel and the second channel are connected, thereby exhausting the gas. When the air pressure in the tube body drops, the sealing ball moves back and forth under the action of the guide slope, thereby blocking the first channel and preventing external gas from entering. BRIEF DESCRIPTION OF THE DRAWINGS
[0017] The accompanying drawings are used to provide a further understanding of the present invention and constitute a part of the specification. Together with the embodiments of the present invention, they are used to explain the present invention and do not constitute a limitation of the present invention. In the accompanying drawings:
[0018] Figure 1 This is a schematic diagram of the structure of a highly practical PCR eight-tube strip of the present invention;
[0019] Figure 2 It is a structural schematic diagram of a one-way gas outlet mechanism of a PCR eight-tube strip with high practicality according to the present invention;
[0020] Figure 3 It is a structural schematic diagram of a temperature detection head of a PCR eight-tube strip with high practicality according to the present invention;
[0021] Figure 4 The present invention is a system block diagram of a PCR eight-tube strip with high practicality.
[0022] In the figure: 1. pipe body; 2. connecting plate; 4. flexible sheet; 5. pipe cover; 6. plug block; 7. guide part; 8. exhaust pipe; 9. one-way air outlet mechanism; 10. temperature measuring frame; 11. collar; 12. connecting frame; 13. positioning shell; 14. processor module; 15. temperature acquisition interface; 16. connecting pipe; 17. temperature detection head; 18. temperature detection probe; 19. insulating block; 20. wire; 21. plug; 22. communication module; 23. lower connecting block; 24. upper connecting block; 25. first groove; 26. second groove; 27. sealing ball; 28. first channel; 29. guide slope; 30. second channel; 31. butt tube. DETAILED DESCRIPTION
[0023] The preferred embodiments of the present invention are described below in conjunction with the accompanying drawings. It should be understood that the preferred embodiments described herein are only used to illustrate and explain the present invention, and are not used to limit the present invention.
[0024] Example: Figure 1 , Figure 2 , Figure 3 and Figure 4 As shown, the present invention provides a highly practical PCR eight-row tube, comprising eight tube bodies 1 connected as one, the upper ends of the tube bodies 1 are connected as one via a connecting plate 2, the upper end of each tube body 1 is connected to a tube cover 5 via a flexible sheet 4, and the tube cover 5 is provided with a plug 6 plugged into the upper end of the tube body 1, and the front end of the plug 6 is provided with a guide portion 7, the plug 6 is interference fit with the upper end of the tube body 1, the tube cover 5 is provided with an exhaust pipe 8, and the exhaust pipe 8 is provided with a one-way air outlet mechanism 9;
[0025] The temperature measuring frame 10 is used to measure the temperature inside each tube body 1, and the temperature measuring frame 10 includes a ring 11 which is sleeved on the outside of the tube body 1 and lifts the tube body. Each ring 11 is connected to form a whole through a connecting frame 12, and the temperature measuring frame 10 is provided with Positioning shell 13, and the positioning housing 13 is provided with a processor module 14, and the temperature measuring frame 10 is provided with eight temperature collection interfaces 15 connected to the processor module 14;
[0026] Each of the tube bodies 1 is provided with a connecting pipe 16 connected to the inside of the tube body 1, and each of the connecting pipes 16 is provided with a temperature detection head 17 for disassembly mechanism; the processor module 14 is provided with a communication module 22, and the processor module 14 transmits the temperature data detected by each temperature detection probe 18 to the monitoring terminal through the communication module 22. First, the temperature detection head 17 and the one-way air outlet mechanism 9 are disinfected. After the disinfection is completed, the temperature detection head 17 is installed on the connecting pipe 16 at the bottom of the tube body 1, and the one-way air outlet mechanism 9 is installed on the exhaust pipe 8, and then the reagent is installed on the tube body 1, and the tube cover 5 is covered. The tube body 1 is placed on the temperature measuring rack 10, and the plug 21 of each temperature detection head 17 is inserted into the temperature collection interface 15. When in use, the temperature detection head 17 detects and collects the reagent temperature in each tube body 1, and the one-way air outlet mechanism 9 performs one-way exhaust when gas expansion occurs in the tube body 1 to avoid excessive pressure, which causes the tube cover to open and the reagent to be contaminated.
[0027] Among them, the one-way air outlet mechanism 9 includes a lower connecting block 23 and an upper connecting block 24, and a first groove 25 is provided in the lower connecting block 22, and a second groove 26 is provided on the upper connecting block 24, and the first groove 25 and the second groove 26 are connected to form a cavity, a sealing ball 27 is provided in the cavity, and a first channel 28 is provided on the side of the first groove 23 on the lower connecting block 23, and a guide slope 29 inclined toward the first channel 28 is provided at the bottom of the first groove 25, and a second channel 30 is provided on the upper connecting block 24, and the upper connecting block 24 and the lower connecting block 23 are threadedly connected. By setting a specific one-way air outlet mechanism 9, the upper connecting block 24 and the lower connecting block 23 are threadedly connected, which has the characteristics of simple structure and easy disassembly and disinfection. When the one-way air outlet mechanism 9 generates gas expansion in the tube body 1, the gas passes through the first upper top and rolls the dynamic sealing ball 27 to one side, so that the first channel 28 and the second channel 30 are connected, so as to exhaust. When the air pressure in the tube body 1 drops, the sealing ball 27 moves back and forth under the action of the guide slope 29, so as to block the first channel 28 and prevent external gas from entering.
[0028] Among them, the lower connecting block 23 is provided with a butt joint 31 connected to the exhaust pipe 8, and the butt joint 31 is threadedly connected to the exhaust pipe 6. The size of the cavity is slightly larger than the size of the sealing ball 27, so that it is convenient for the sealing ball to open the channel when pushed by air pressure.
[0029] The sealing ball 27 is a steel ball, which falls back by its own gravity, thus having a better sealing effect.
[0030] Among them, the temperature detection head 17 includes a temperature detection probe 18, and the outside of the temperature detection probe 18 is provided with an insulating block 19 that wraps the outer end of the temperature detection probe 18, the temperature detection probe 18 is connected to a wire 20 extending from the insulating block 19, and the outer end of the wire 20 is provided with a plug 21 inserted into the temperature acquisition interface 15; the disassembly mechanism includes an external thread structure provided on the insulating block 19, and the insulating block 19 is threadedly connected to the connecting pipe 16, so that the temperature detection head 17 is easy to disassemble and convenient for disinfection, and no dead corners will remain during disinfection, which is convenient for subsequent reuse.
[0031] The connecting pipe 16 is arranged at the bottom of the pipe body 1, so as not to cause adverse effects on normal use.
[0032] During operation, the PCR eight-row tube with high practicality is first disinfected for the temperature detection head 17 and the one-way air outlet mechanism 9. After the disinfection is completed, the temperature detection head 17 is installed on the connecting tube 16 at the bottom of the tube body 1, and the one-way air outlet mechanism 9 is installed on the exhaust pipe 8. Then, the reagent is loaded on the tube body 1, and the tube cover 5 is covered. The tube body 1 is placed on the temperature measuring rack 10, and the plugs 21 of each temperature detection head 17 are inserted into the temperature collection interface 15. When in use, the temperature detection head 17 detects and collects the reagent temperature in each tube body 1. When the one-way air outlet mechanism 9 generates gas expansion in the tube body 1, the gas is pushed up from the first passage, and the dynamic sealing ball 27 rolls to one side, so that the first channel 28 and the second channel 30 are connected, so as to exhaust. When the air pressure in the tube body 1 drops, the sealing ball 27 moves back and forth under the action of the guide slope 29, so as to block the first channel 28 and prevent external gas from entering, thereby reducing the influence of temperature and making the experimental data more accurate.
[0033] Finally, it should be noted that the above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or replace some of the technical features therein by equivalents. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.
Claims
1. A highly practical PCR eight-tube strip, characterized by: The invention comprises eight tube bodies (1) connected as one body, wherein the upper ends of the tube bodies (1) are connected as one body via a connecting plate (2), the upper end of each tube body (1) is connected to a tube cover (5) via a flexible sheet (4), and the tube cover (5) is provided with a plug (6) plugged into the upper end of the tube body (1), and a guide portion (7) is provided at the front end of the plug (6), the plug (6) and the upper end of the tube body (1) are interference fit, the tube cover (5) is provided with an exhaust pipe (8), and the exhaust pipe (8) is provided with a one-way air outlet mechanism (9); The invention also comprises a temperature measuring frame (10) for measuring the temperature inside each tube body (1), wherein the temperature measuring frame (10) comprises a sleeve (11) provided on a support and sleeved outside the tube body (1) and used to lift the tube body, each sleeve (11) being connected to form an integral body via a connecting frame (12), and the temperature measuring frame (10) is provided with Positioning Shell( 13), and a processor module (14) is provided on the positioning housing (13), and eight temperature collection interfaces (15) connected to the processor module (14) are provided on the temperature measuring frame (10); Each of the tube bodies (1) is provided with a connecting tube (16) connected to the interior of the tube body (1), and each of the connecting tubes (16) is provided with a temperature detection head (17) for mounting a disassembly mechanism; a communication module (22) is provided on the processor module (14), and the processor module (14) transmits temperature data detected by each temperature detection probe (18) to a monitoring terminal via the communication module (22).
2. The highly practical PCR eight-tube strip according to claim 1, characterized in that: The one-way air outlet mechanism (9) comprises a lower connecting block (23) and an upper connecting block (24), wherein a first groove (25) is provided in the lower connecting block (22), and a second groove (26) is provided on the upper connecting block (24), and the first groove (25) and the second groove (26) are butted against each other to form a cavity, wherein a sealing ball (27) is provided in the cavity, and a first channel (28) is provided on the side of the first groove (23) on the lower connecting block (23), and a guide slope (29) inclined toward the first channel (28) is provided at the bottom of the first groove (25), and a second channel (30) is provided on the upper connecting block (24), and the upper connecting block (24) and the lower connecting block (23) are threadedly connected.
3. The highly practical PCR eight-tube strip according to claim 2, characterized in that: The lower connection block (23) is provided with a butt joint pipe (31) connected to the exhaust pipe (8), and the butt joint pipe (31) is threadedly connected to the exhaust pipe (6), and the size of the cavity is slightly larger than the size of the sealing ball (27).
4. The highly practical PCR eight-tube strip according to claim 2, characterized in that: The sealing ball (27) is a steel ball.
5. The highly practical PCR eight-tube strip according to claim 1, characterized in that: The temperature detection head (17) comprises a temperature detection probe (18), and an insulating block (19) is provided on the outside of the temperature detection probe (18) to wrap the outer end of the temperature detection probe (18); the temperature detection probe (18) is connected to a wire (20) extending from the insulating block (19), and the outer end of the wire (20) is provided with a plug (21) inserted into the temperature acquisition interface (15); the disassembly mechanism comprises an external thread structure provided on the insulating block (19), and the insulating block (19) is threadedly connected to the connecting pipe (16).
6. The highly practical PCR eight-tube strip according to claim 5, characterized in that: The connecting pipe (16) is arranged at the bottom of the pipe body (1).
7. A highly practical PCR eight-tube strip according to any one of claims 1 to 6, characterized in that: The working method of the PCR eight-tube strip is as follows: first, the temperature detection head and the one-way air outlet mechanism are disinfected. After the disinfection is completed, the temperature detection head is installed on the connecting pipe at the bottom of the tube body, and the one-way air outlet mechanism is installed on the exhaust pipe. Then, the reagent is installed on the tube body, and the tube cover is covered. The tube body is placed on a temperature measuring rack, and the plugs of each temperature detection head are inserted into the temperature collection interface. When in use, the temperature detection head detects and collects the reagent temperature in each tube body, and when the one-way air outlet mechanism generates gas expansion in the tube body, the gas is pushed up from the first passage, and the dynamic sealing ball rolls to one side, thereby connecting the first channel with the second channel, thereby exhausting the gas. When the air pressure in the tube body drops, the sealing ball moves back and forth under the action of the guide slope, thereby blocking the first channel and preventing external gas from entering.