Automatic microorganism coating device

Abstract

The invention belongs to the technical field of microorganism culture, and particularly relates to an automatic microorganism coating device which comprises a base, a dilution liquid supply mechanism, a coating mechanism and a drying mechanism, and the top of the base is provided with a mounting frame and a culture dish; when the dilution liquid supply mechanism works, the bacterial liquid is automatically subjected to sealed gradient dilution, and then the bacterial liquid subjected to gradient dilution is conveyed into the culture dish; when the diluting and liquid supplying mechanism conveys bacterial liquid into the culture dish, the coating mechanism is automatically driven to start to work, and uniform coating operation is carried out on the bacterial liquid in the culture dish; when the coating mechanism works, the drying mechanism is automatically driven to start to work to dry the bacterial liquid in the culture dish, the automatic microorganism coating device replaces a traditional microorganism coating device to conduct coating, and the situation that when a strain is subjected to coating operation, the coating efficiency is greatly improved is avoided. The problem of poor coating effect caused by non-uniform coating and influence on the culture effect of the target strain due to cross infection of the strain is solved.

Description

Technical Field

[0001] The invention relates to the technical field of microorganism cultivation, in particular to an automatic microorganism coating device. Background Art

[0002] Spreading inoculation is a commonly used microbial culture method. It can not only be used for microbial counting and separation, but also for observing microorganisms by taking advantage of their characteristic of growing and forming colonies on the surface of plates. In traditional microbial culture technology, the microbial spreading operation requires first diluting the bacterial solution in a series of gradients, and then spreading the bacterial solutions of different dilutions on the surface of agar solid culture medium. The spreading operation requires the use of culture dishes and spreading rods. When used, the operator drops an appropriate amount of bacterial solution on the culture medium of the culture dish, and uses a spreading rod to spread the bacterial solution back and forth on the surface of the culture medium, so that a single colony or bacterial moss grows on the culture medium, achieving the purpose of separation, counting or culture observation. At present, in order to save manpower and improve the spreading efficiency, it is generally operated by an automatic coating machine.

[0003] Before applying the microbial strains, the bacterial solution generally needs to be diluted. However, the current coating device requires manual step-by-step operation for the gradient dilution of the bacterial solution before coating, which is time-consuming and has a high risk of cross-contamination. Infection with foreign bacteria will cause confusion in the bacterial solution, which can easily lead to uneven coating and affect the cultivation of the target strain, resulting in poor coating effect. Summary of the invention

[0004] The purpose of this section is to summarize some aspects of the embodiments of the present invention and briefly introduce some preferred embodiments. Some simplifications or omissions may be made in this section and the specification abstract and the invention title of this application to avoid blurring the purpose of this section, the specification abstract and the invention title, and such simplifications or omissions cannot be used to limit the scope of the present invention.

[0005] Therefore, the purpose of the present invention is to provide an automatic microbial coating device to replace the traditional microbial coating device for coating, thereby avoiding the problem of cross-infection of bacteria during the coating operation, resulting in uneven coating and affecting the target bacterial culture effect, thereby resulting in poor coating effect.

[0006] To solve the above technical problems, according to one aspect of the present invention, the present invention provides the following technical solutions: An automatic microbial coating device, comprising: A base having a mounting frame and a culture dish on the top thereof; A dilution liquid supply mechanism, which is installed on the mounting frame, wherein the dilution liquid supply mechanism automatically performs sealed gradient dilution on the bacterial solution and then delivers the gradient diluted bacterial solution to the culture dish when in operation; a coating mechanism, which is mounted on the mounting frame and corresponds to the culture dish, wherein when the dilution and liquid supply mechanism delivers bacterial liquid into the culture dish, the coating mechanism is automatically driven to start working, and a coating operation is performed evenly on the bacterial liquid in the culture dish; The drying mechanism is installed on the coating mechanism, wherein when the coating mechanism is in operation, the drying mechanism is automatically driven to start working to dry the bacterial liquid in the culture dish.

[0007] As a preferred embodiment of the automatic microbial coating device described in the present invention, the dilution and liquid supply mechanism includes a gradient dilution component and a liquid supply drive component connected to the output end of the gradient dilution component and driving the coating mechanism to work when in operation.

[0008] As a preferred embodiment of the automatic microbial coating device described in the present invention, the gradient dilution component includes a main liquid storage tank installed on the mounting frame, a connecting tube detachably connected to the output end of the main liquid storage tank and having a hydrophobic filter membrane inside, and a buffer tank whose input end is detachably connected to the other end of the connecting tube and has a liquid outlet at the bottom.

[0009] As a preferred solution of the automatic microbial coating device described in the present invention, the liquid supply drive assembly includes a first mounting plate located on the mounting frame, a swinging member corresponding to the liquid outlet, and a driving member installed on one side of the first mounting plate and having one end transmission connected to the swinging member and the other end transmission connected to the coating mechanism.

[0010] As a preferred embodiment of the automatic microorganism coating device of the present invention, the swing member includes an eccentric pendulum corresponding to the liquid outlet and a swing block located at one end of the eccentric pendulum; The driving member includes a ratchet disk rotatably mounted on the side wall of the first mounting plate and having a first torsion spring on the connecting shaft, a driving ratchet mounted on one side of the first mounting plate, a first bevel gear set connected to the driving ratchet through the rotating shaft at one end, and a pulley set connected to the output end of the first bevel gear set through a pulley transmission at one end, one end of the swing member is rotatably connected to the inner wall of the ratchet disk and has a second torsion spring on the connected rotating shaft, the swing block corresponds to the elastic pawl on the inner side of the ratchet disk, the outer side wall of the ratchet disk has a plurality of first serrations and a side adjacent to the first serrations has an extrusion block, and the outer side wall of the ratchet disk has second serrations corresponding to the elastic pawl of the driving ratchet; The side wall of the first mounting plate has an escapement, which includes a first mounting seat located on the side wall of the first mounting plate and having a first limiting groove on the inner wall, an elastic limiting pawl located in the first limiting groove and corresponding to the first sawtooth, and a ramp block located at the bottom of the elastic limiting pawl and corresponding to the extrusion block, and the side wall of the elastic limiting pawl has a first elastic member connected to the inner wall of the first mounting seat at the other end.

[0011] As a preferred embodiment of the automatic microbial coating device described in the present invention, the coating mechanism includes a second mounting plate mounted on the mounting frame, a coating assembly located on the first mounting plate, and a first transmission assembly having one end transmission connected to the driving member and the other end transmission connected to the coating assembly.

[0012] As a preferred solution of the automatic microorganism coating device described in the present invention, the coating assembly includes a rotating spindle located at the bottom of the second mounting plate, a fixed plate located at the bottom of the rotating spindle, and a coating rod with a plurality of second elastic members on the top, and the other end of the second elastic member is connected to the bottom of the fixed plate; The first transmission assembly includes a second bevel gear set located on the top of the second mounting plate and connected to the rotating main shaft at one end, and a transmission pulley at one end connected to the other end of the second bevel gear set and connected to the pulley set through a belt.

[0013] As a preferred solution of the automatic microorganism coating device described in the present invention, the bottom of the fixed plate has an air guide groove, the top of the fixed plate has a second limiting slide groove, the top of the fixed plate has a connecting tube with one end connected to the air guide groove, and the top of the fixed plate has a counterweight bar extending into the second limiting slide groove and slidably connected to the inner wall of the connecting tube; The top of the coating rod is provided with a plug corresponding to the air guide groove.

[0014] As a preferred solution of the automatic microorganism coating device of the present invention, the end of the counterweight bar has a sawtooth block; The bottom of the buffer bin is provided with an on-off assembly for opening and closing the liquid outlet, and the on-off assembly includes a second mounting seat located at the bottom of the buffer bin and having a third limiting sliding groove on the inner wall, a magnetic attraction plate located at one side of the second mounting seat, and a partition extending into the third limiting sliding groove and having a limiting hole at the bottom; A trigger assembly is installed on the second mounting plate, and the trigger assembly includes a transmission gear located at the bottom of the second mounting plate and meshing with the sawtooth block, a threaded rod located at the top of the second mounting plate and connected to the transmission gear through a rotating shaft, and a trigger frame with one end threadedly sleeved on the threaded rod and slidably sleeved on the limiting sliding rod at the top of the second mounting plate, and the top of the trigger frame has a limiting protrusion corresponding to the limiting hole.

[0015] As a preferred embodiment of the automatic microbial coating device described in the present invention, the drying mechanism includes a vortex tube installed on the second mounting plate and having a guide tube at the output end, and a second transmission assembly connected to the rotating main shaft at one end and connected to the vortex tube at the other end, and the reverse direction of the guide tube forms an angle of thirty degrees with the coating direction of the coating rod; The second transmission assembly includes a connecting frame located at the bottom of the second mounting plate, a trigger plate hinged on the connecting frame, a trigger switch located on the side wall of the connecting frame and corresponding to the trigger plate, and an eccentric wheel located on the side wall of the rotating spindle and corresponding to the bottom of the trigger plate, and the trigger switch is electrically connected to the vortex tube.

[0016] Compared with the prior art, the present invention has the beneficial effect that the automatic microbial coating device automatically gradient dilutes the bacterial solution in a sealed environment through a dilution liquid supply mechanism and then delivers it to a culture dish, thereby reducing cross-infection between bacterial species. When the gradient diluted bacterial solution is output, the coating mechanism is driven to evenly coat the bacterial solution in the culture dish. At the same time, the drying mechanism works to dry the bacterial solution in the culture dish, so that during the coating process, the moisture in the bacterial solution is quickly dried up, which prevents the bacterial species from flowing freely and easily stacking and aggregating, thereby causing uneven distribution of the coated bacterial species. The coating method of replacing the traditional microbial coating device is avoided, and the problem of uneven coating caused by cross-infection of bacterial species during the coating operation of the bacterial species is avoided, which affects the culture effect of the target bacterial species and causes poor coating effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the present invention will be described in detail below in combination with the accompanying drawings and detailed embodiments. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative labor. Among them: Figure 1 It is a structural schematic diagram of an automatic microorganism coating device of the present invention; Figure 2 This is a structural breakdown diagram of an automatic microbial coating device of the present invention; Figure 3This is a structural breakdown diagram of a gradient dilution component of an automatic microbial coating device of the present invention; Figure 4 A structural disassembled diagram of a liquid supply drive component of an automatic microorganism coating device of the present invention from one perspective; Figure 5 A structural disassembled diagram of a liquid supply drive assembly of an automatic microorganism coating device of the present invention from another perspective; Figure 6 This is a disassembled diagram of the connection structure between the coating mechanism and the drying mechanism of an automatic microorganism coating device of the present invention; Figure 7 The figure is a schematic diagram of the connection structure between the second mounting plate and the trigger assembly of an automatic microorganism coating device of the present invention.

[0018] In the figure: 100, base; 110, mounting frame; 120, culture dish; 200, dilution supply mechanism; 210, gradient dilution assembly; 210a, main liquid storage tank; 210b, connecting pipe; 210c, buffer tank; 210c-1, liquid outlet; 210c-2, on-off assembly; 210c-21, second mounting seat; 210c-22, magnetic suction plate; 210c-23, partition; 210c-231, limit hole; 22 0, liquid supply drive assembly; 220a, first mounting plate; 220a-1, escapement; 220a-11, first mounting seat; 220a-12, elastic limit pawl; 220a-13, inclined block; 220b, swing member; 220b-1, eccentric pendulum; 220b-2, swing block; 220c, drive member; 220c-1, ratchet disk; 220c-11, first sawtooth; 220c-12, extrusion block; 220 c-13, second sawtooth; 220c-2, driving ratchet; 220c-3, first bevel gear set; 220c-4, pulley set; 300, coating mechanism; 310, second mounting plate; 310a, trigger assembly; 310a-1, transmission gear; 310a-2, threaded rod; 310a-3, trigger frame; 310a-31, limit convex block; 320, coating assembly; 320a, rotating spindle; 320b, fixing plate ; 320b-1, air guide groove; 320b-2, counterweight bar; 320b-21, sawtooth block; 320b-3, connecting tube; 320c, coating rod; 320c-1, second elastic member; 320c-2, plug column; 400, drying mechanism; 410, vortex tube; 410a, guide tube; 420, second transmission assembly; 420a, connecting frame; 420b, trigger plate; 420c, trigger switch; 420d, eccentric wheel. DETAILED DESCRIPTION

[0019] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the specific embodiments of the present invention are described in detail below with reference to the accompanying drawings.

[0020] Secondly, the present invention is described in detail with reference to schematic diagrams. When describing the embodiments of the present invention in detail, for the sake of convenience, the cross-sectional diagrams showing the device structure will not be partially enlarged according to the general scale, and the schematic diagrams are only examples, which should not limit the scope of protection of the present invention. In addition, in actual production, the three-dimensional dimensions of length, width and depth should be included.

[0021] In order to make the objectives, technical solutions and advantages of the present invention more clear, the embodiments of the present invention will be further described in detail below with reference to the accompanying drawings.

[0022] The present invention provides an automatic microbial coating device, which replaces the traditional microbial coating device for coating, avoiding the problem of cross-infection of bacteria during the coating operation, resulting in uneven coating and affecting the target bacteria culture effect, thereby resulting in poor coating effect.

[0023] Figure 1-Figure 7 The structure diagram of an automatic microorganism coating device of the present invention is shown in FIG. Figure 1-Figure 7 A detailed introduction is given to this automatic microbial coating device.

[0024] Example 1: Reference Figure 1-Figure 2 The present invention discloses an automatic microorganism coating device, the main body of which includes a base 100, a dilution and liquid supply mechanism 200, a coating mechanism 300 and a drying mechanism 400.

[0025] refer to Figure 1-Figure 2 The base 100 is used to support the entire device, and the top thereof is provided with a mounting frame 110 and a culture dish 120. The mounting frame 110 is used to facilitate the installation of the dilution and liquid supply mechanism 200, the coating mechanism 300 and the drying mechanism 400, and the culture dish 120 is used to carry the bacterial colonies to be cultured; refer to Figure 1-Figure 2 The dilution liquid supply mechanism 200 is used to seal and dilute the bacterial solution and then introduce it into the culture dish 120 for coating. The dilution liquid supply mechanism 200 is installed on the mounting frame 110. When the dilution liquid supply mechanism 200 is working, it automatically performs a sealed gradient dilution on the bacterial solution and then transports the gradient diluted bacterial solution into the culture dish 120, so that the bacterial solution is automatically gradient diluted in a sealed ring environment to avoid cross infection of bacterial species. refer to Figure 1-Figure 2The coating mechanism 300 is used to coat the bacterial solution dripping into the culture dish 120. The coating mechanism 300 is mounted on the mounting frame 110 and corresponds to the culture dish 120. When the dilution liquid supply mechanism 200 delivers the bacterial solution into the culture dish 120, the coating mechanism 300 is automatically driven to start working, and the bacterial solution in the culture dish 120 is evenly coated. Thus, when the bacterial solution is gradiently diluted and drips into the culture dish 120, the coating mechanism 300 is automatically driven to start working, and the bacterial colonies dripping into the culture dish 120 are evenly coated. refer to Figure 1-Figure 2 The drying mechanism 400 is used to dry the bacterial solution being coated in the culture dish 120. The drying mechanism 400 is installed on the coating mechanism 300. When the coating mechanism 300 is working, the drying mechanism 400 is automatically driven to start working to dry the bacterial solution in the culture dish 120. When the coating mechanism 300 is working, the drying mechanism 400 is automatically driven to synchronously dry the bacterial solution being coated in the culture dish 120, so as to quickly dry the water in the bacterial solution, and avoid the bacterial species from freely flowing and laminating in the bacterial solution, resulting in uneven distribution of the bacterial species, thereby causing uneven coating.

[0026] In this embodiment, the specific use process is as follows: the bacterial liquid is automatically gradient diluted in a sealed environment by the dilution liquid supply mechanism 200 and then dripped into the culture dish 120. At the same time, when the bacterial liquid drips, it drives the coating mechanism 300 to work, and evenly coats the bacterial liquid dripped into the culture dish 120. When the coating mechanism 300 works, it automatically drives the drying mechanism 400 to work, and synchronously dries the bacterial liquid being coated, so as to quickly remove the moisture in the bacterial liquid, and avoid the bacteria species from freely flowing in the bacterial liquid and then overlapping, which leads to uneven distribution of bacteria species and uneven coating.

[0027] Example 2: Based on Example 1, Figure 1-Figure 5 The dilution liquid supply mechanism 200 includes a gradient dilution component 210 and a liquid supply driving component 220 connected to the output end of the gradient dilution component 210 and driving the coating mechanism 300 to work when working. The gradient dilution component 210 is used to automatically perform gradient dilution on the bacterial solution in a sealed environment, and the liquid supply driving component 220 is used to drive the coating mechanism 300 to work after the diluted bacterial solution is discharged.

[0028] In this embodiment, reference Figure 3The gradient dilution assembly 210 includes a main liquid storage tank 210a installed on the mounting frame 110, a connecting tube 210b detachably connected to the output end of the main liquid storage tank 210a and having a hydrophobic filter membrane inside, and a buffer tank 210c whose input end is detachably connected to the other end of the connecting tube 210b and has a liquid outlet 210c-1 at the bottom. The main liquid storage tank 210a is used to store the bacterial liquid stock solution. The hydrophobic filter membrane inside the connecting tube 210b balances the air pressure difference between the main liquid storage chamber and the buffer chamber through its characteristics and blocks bacteria to avoid cross infection. At the same time, it makes the flow rate of the bacterial liquid entering the buffer tank 210c more uniform to avoid siphoning. The buffer tank 210c is used to temporarily store and then export the bacterial liquid that passes through the hydrophobic filter membrane. The liquid outlet 210c-1 is used to drip the bacterial liquid in the buffer tank 210c.

[0029] In this embodiment, reference Figure 1-Figure 5 The liquid supply drive assembly 220 includes a first mounting plate 220a located on the mounting frame 110, a swinging member 220b corresponding to the liquid outlet 210c-1, and a driving member 220c installed on one side of the first mounting plate 220a and having one end drivingly connected to the swinging member 220b and the other end drivingly connected to the coating mechanism 300. The first mounting plate 220a is used to facilitate the installation of the swinging member 220b and the driving member 220c. The swinging member 220b is used to drive the swinging member 220b to swing when the bacterial liquid dripping from the liquid outlet 210c-1 falls, and then drive the driving member 220c to work. The driving member 220c is used to drive the coating mechanism 300 to work when working.

[0030] In this embodiment, reference Figure 4-Figure 5 The swing member 220b includes an eccentric pendulum 220b-1 corresponding to the liquid outlet 210c-1 and a swing block 220b-2 located at one end of the eccentric pendulum 220b-1. The eccentric pendulum 220b-1 is used to receive the dripping bacterial liquid and then drive the swing block 220b-2 to swing. The swing block 220b-2 is used to drive the ratchet 220c-2 disk 220c-1 to deflect once when swinging once; The driving member 220c includes a ratchet disc 220c-1 rotatably mounted on the side wall of the first mounting plate 220a and having a first torsion spring on the connecting shaft, a driving ratchet 220c-2 mounted on one side of the first mounting plate 220a, a first bevel gear set 220c-3 connected to the driving ratchet 220c-2 at one end through a rotating shaft, and a pulley set 220c-4 connected to the output end of the first bevel gear set 220c-3 at one end through a pulley transmission. The ratchet disc 220c-1 is used as an elastic limit ratchet When the claw 220a-12 is released from the limit and reverses, it drives the driving ratchet 220c-2 to rotate. The driving ratchet 220c-2 is used to drive the first bevel gear set 220c-3 to rotate when rotating. The first bevel gear set 220c-3 is used to drive the pulley set 220c-4 to rotate when rotating. The pulley set 220c-4 is used to drive the coating mechanism 300 to work when rotating. One end of the swinging member 220b is rotatably connected to the inner wall of the ratchet disk 220c-1 and a second torsion spring is provided on the connected rotating shaft. , which is used to make the swing block 220b-2 and the eccentric pendulum 220b-1 automatically return to their original state after swinging once through their own elastic force. The swing block 220b-2 corresponds to the elastic pawl on the inner side of the ratchet disc 220c-1. The outer wall of the ratchet disc 220c-1 has a plurality of first saw teeth 220c-11 and a squeezing block 220c-12 is provided on the side adjacent to the first saw teeth 220c-11. When the ratchet disc 220c-1 is deflected to a certain angle, the squeezing block 220c-12 squeezes After the inclined surface block 220a-13 is pressed, the elastic limiting pawl 220a-12 is pushed to move along the first limiting sliding groove, and the restriction on the first sawtooth 220c-11 is released, so that the ratchet disc 220c-1 is driven to reverse under the torsion of the first torsion spring. The outer wall of the ratchet disc 220c-1 has a second sawtooth 220c-13 corresponding to the elastic pawl of the driving ratchet 220c-2, which is used to drive the driving ratchet 220c-2 to rotate when the ratchet disc 220c-1 is reversed; refer to Figure 4-Figure 5The side wall of the first mounting plate 220a has an escapement 220a-1, which is used to cooperate with the first sawtooth 220c-11 to limit the ratchet disc 220c-1 when the ratchet disc 220c-1 is deflected to a certain angle, so as to facilitate the first torsion spring to be tightened. The escapement 220a-1 includes a first mounting seat 220a-11 located on the side wall of the first mounting plate 220a and having a first limiting groove on the inner wall, an elastic limiting pawl 220a-12 located in the first limiting groove and corresponding to the first sawtooth 220c-11, and an inclined block 220a-13 located at the bottom of the elastic limiting pawl 220a-12 and corresponding to the extrusion block 220c-12, and the elastic limiting pawl 220a-12 is provided. The side wall of 0a-12 has a first elastic member whose other end is connected to the inner wall of the first mounting seat 220a-11. The first mounting seat 220a-11 is used to facilitate the sliding installation of the elastic limiting pawl 220a-12. The elastic limiting pawl 220a-12 is used to limit the first saw tooth 220c-11. The inclined block 220a-13 is used to force the elastic limiting pawl 220a-12 to move along the first limiting groove when it contacts the extrusion block 220c-12. The first elastic member is used to force the elastic limiting pawl 220a-12 to limit the first saw tooth 220c-11 under its own elastic force when the inclined block 220a-13 is not in contact with the extrusion block 220c-12.

[0031] In this embodiment, the specific working process is as follows: the bacterial liquid in the main liquid storage tank 210a passes through the hydrophobic filter membrane in the connecting tube 210b and enters the buffer tank 210c, thereby completing the gradient dilution and avoiding cross infection with bacteria. The bacterial liquid in the buffer tank 210c drips through the liquid outlet 210c-1 and hits the eccentric pendulum 220b-1. In this process, the surface of the eccentric pendulum 220b-1 is at an obtuse angle to the falling direction of the bacterial liquid, thereby avoiding splashing of the bacterial liquid and causing loss. At this time, the eccentric pendulum 220b-1 and the swing block 220b-2 are driven to swing once, thereby driving the ratchet 220c-2 and the disc 220c-1 to deflect once. The bacterial liquid continues to drip. When the ratchet disk 220c-1 deflects to a certain angle and the extrusion block 220c-12 squeezes the inclined block 220a-13, the elastic limiting pawl 220a-12 moves along the first limiting slide groove to release the limiting of the first sawtooth 220c-11. At this time, the first torsion spring resets and drives the ratchet 220c-2 disk 220c-1 to reverse, driving the driving ratchet 220c-2 to rotate and then drives the first bevel gear set 220c-3 to rotate. When the first bevel gear set 220c-3 rotates, it drives the pulley set 220c-4 to rotate. When the pulley set 220c-4 rotates, it drives the coating mechanism 300 to start working.

[0032] Example 3: Based on Example 2, Figure 1-Figure 7The coating mechanism 300 includes a second mounting plate 310 mounted on the mounting frame 110, a coating assembly 320 located on the first mounting plate 220a, and a first transmission assembly having one end transmission-connected to the driving member 220c and the other end transmission-connected to the coating assembly 320. The second mounting plate 310 is used to facilitate the installation of the coating assembly 320 and the first transmission assembly. The coating assembly 320 is used to coat the bacterial liquid in the culture dish 120 when working, and the first transmission assembly is used to drive the coating assembly 320 to work when working.

[0033] In this embodiment, reference Figure 6 The coating assembly 320 includes a rotating spindle 320a located at the bottom of the second mounting plate 310, a fixed plate 320b located at the bottom of the rotating spindle 320a, and a coating rod 320c having a plurality of second elastic members 320c-1 at the top. The rotating spindle 320a is used to drive the fixed plate 320b to rotate when rotating, and the fixed plate 320b is used to drive the coating rod 320c to rotate when conveniently rotating. The coating rod 320c is used to evenly coat the bacterial solution in the culture dish 120 when rotating. The other end of the second elastic member 320c-1 is connected to the bottom of the fixed plate 320b. The second elastic member 320c-1 is used to prevent excessive pressure between the bottom of the coating rod 320c and the inner wall of the culture dish 120, thereby affecting the rotation of the coating assembly 320; refer to Figure 6 The first transmission assembly includes a second bevel gear set located at the top of the second mounting plate 310 and connected to the rotating main shaft 320a at one end, and a transmission pulley at one end connected to the other end of the second bevel gear set and connected to the pulley set 220c-4 through a belt. The second bevel gear set is used to drive the rotating main shaft 320a to rotate when it rotates, and the transmission pulley is used to drive the second bevel gear set to rotate when the pulley set 220c-4 drives it to rotate.

[0034] In this embodiment, the specific working process is as follows: when the pulley group 220c-4 rotates to drive the transmission pulley to rotate, the transmission pulley drives the second bevel gear group to rotate, and the second bevel gear group rotates to drive the rotating main shaft 320a to rotate, and the rotating main shaft 320a rotates to drive the fixed plate 320b to rotate, thereby driving the coating rod 320c to rotate under the connection action of the second elastic member 320c-1, and performing a uniform coating operation on the bacterial liquid in the culture dish 120.

[0035] Example 4: Based on Example 3, in order to avoid excessive pressure between the culture medium in the culture dish 120 and the coating rod 320c as the coating continues, refer to Figure 6-Figure 7The bottom of the fixed plate 320b has an air guide groove 320b-1, which is used to introduce the squeezed air into the connecting tube 320b-3. The top of the fixed plate 320b has a second limiting slide groove, which is used to facilitate the moving rod of the counterweight bar 320b-2. The top of the fixed plate 320b has a connecting tube 320b-3 with one end connected to the air guide groove 320b-1, which is used to drive the counterweight bar 320b-2 to move along the second limiting slide groove toward a position away from the axis when the high-pressure air in the air guide groove 320b-1 is introduced. The top of the fixed plate 320b has a counterweight bar 320b-2 extending into the second limiting slide groove and slidably connected to the inner wall of the connecting tube 320b-3, which is used to automatically balance the pressure between the coating component 320 and the culture medium of the culture dish 120 through the centrifugal force generated during rotation, thereby maintaining the stability of the coating; refer to Figure 6-Figure 7 The top of the coating rod 320c has a plug post 320c-2 corresponding to the air guide groove 320b-1, which is used when the coating rod 320c and the culture dish 120 become increasingly crowded. As the second elastic member 320c-1 is deformed, the plug post 320c-2 gradually extends into the air guide groove 320b-1, and gradually squeezes the air in the air guide groove 320b-1 into the connecting tube 320b-3.

[0036] In this embodiment, the specific working process is as follows: as the coating continues, when the pressure between the coating rod 320c and the culture medium gradually increases, the plug 320c-2 extends into the air guide groove 320b-1, and the air in the air guide groove 320b-1 is squeezed into the connecting tube 320b-3, thereby driving the counterweight bar 320b-2 to move along the second limiting slide groove toward a position away from the axis, and then through the centrifugal force generated by the rotation of the counterweight bar 320b-2, the pressure between the coating component 320 and the culture medium is automatically balanced.

[0037] Example 5: Based on Example 4, in order to facilitate the pressure between the coating component 320 and the culture medium to reach a certain value to stop the liquid supply, so as to automatically quantify the coated bacterial liquid, refer to Figure 1-Figure 7 The end of the counterweight bar 320b-2 has a sawtooth block 320b-21, which is used to drive the transmission gear 310a-1 to rotate through the sawtooth block 320b-21 when the counterweight bar 320b-2 moves to a certain position and the counterweight bar 320b-2 rotates; refer to Figure 3The bottom of the buffer bin 210c has an on-off assembly 210c-2 for opening and closing the liquid outlet 210c-1. The on-off assembly 210c-2 includes a second mounting seat 210c-21 located at the bottom of the buffer bin 210c and having a third limiting groove on the inner wall, a magnetic plate 210c-22 located on one side of the second mounting seat 210c-21, and a partition 210c-23 extending into the third limiting groove and having a limiting hole 210c-231 at the bottom. The second mounting seat 210c-21 is used to facilitate the installation of the magnetic plate 210c-22 and the partition Plate 210c-23, the partition 210c-23 is used to block the liquid outlet 210c-1 when combined with the magnetic plate 210c-22, the partition 210c-23 has a magnetic sheet on one side adjacent to the magnetic plate 210c-22, the magnetic plate 210c-22 is used to adsorb the partition 210c-23 to the position for blocking the liquid outlet 210c-1 when the partition 210c-23 is released from the limit, the limiting hole 210c-231 is used to cooperate with the limiting protrusion 310a-31 to limit the partition 210c-23; refer to Figure 7 A trigger assembly 310a is installed on the second mounting plate 310, which is used to trigger the on-off assembly 210c-2 to block the liquid outlet 210c-1 when the pressure between the coating assembly 320 and the culture medium reaches a certain value. The trigger assembly 310a includes a transmission gear 310a-1 located at the bottom of the second mounting plate 310 and meshed with the sawtooth block 320b-21, a threaded rod 310a-2 located at the top of the second mounting plate 310 and connected to the transmission gear 310a-1 through a rotating shaft, and a trigger assembly 310a having one end threadedly sleeved on the threaded rod 310a-2 and slidably sleeved on the limiting sliding rod at the top of the second mounting plate 310. The trigger frame 310a-3 has a limiting protrusion 310a-31 on the top corresponding to the limiting hole 210c-231. The transmission gear 310a-1 is used to drive the threaded rod 310a-2 to rotate when it rotates. The threaded rod 310a-2 is used to drive the trigger frame 310a-3 to move downward under the limiting action of the limiting slide rod when it rotates. The trigger frame 310a-3 is used to drive the limiting protrusion 310a-31 away from the limiting hole 210c-231 when it moves downward. The limiting protrusion 310a-31 is used to cooperate with the limiting hole 210c-231 to limit the partition 210c-23.

[0038] In this embodiment, the specific working process is as follows: when the pressure between the coating component 320 and the culture medium reaches a certain value, the counterweight bar 320b-2 moves to the farthest end away from the axis. As the counterweight bar 320b-2 rotates, the transmission gear 310a-1 is driven to rotate through the sawtooth block 320b-21. When the transmission gear 310a-1 rotates, it drives the threaded rod 310a-2 to rotate. When the threaded rod 310a-2 rotates, it drives the trigger frame 310a-3 to move downward under the limiting action of the limiting slide rod, thereby driving the limiting protrusion 310a-31 to move out of the limiting hole 210c-231. At this time, the partition 210c-23 moves toward the magnetic suction plate 210c-22 along the third limiting slide groove under the suction force of the magnetic suction plate 210c-22, and blocks the liquid outlet 210c-1, thereby completing the quantitative release of the coated bacterial liquid.

[0039] Example 6: Based on Example 5, Figure 1-Figure 6 The drying mechanism 400 includes a vortex tube 410 installed on the second mounting plate 310 and having a guide tube 410a at the output end, and a second transmission assembly 420 with one end being transmission-connected to the rotating main shaft 320a and the other end being transmission-connected to the vortex tube 410. The vortex tube 410 is used to release hot air flow into the guide tube 410a during operation. At the same time, in order to prevent the hot air flow from being too high in temperature and causing damage to the bacteria, the staff sets the output temperature of the vortex tube 410 in advance. The guide tube 410a is used to guide the hot air flow into the culture dish 120, so as to dry the moisture in the bacterial liquid. The second transmission assembly 420 is used to trigger the vortex tube 410 to work when the coating assembly 320 is working. The reverse direction of the guide tube 410a is at an angle of thirty degrees to the coating direction of the coating rod 320c, so as to produce a Coanda effect to adsorb the miscellaneous bacteria particles in the internal environment of the culture dish 120. refer to Figure 6-Figure 7 The second transmission assembly 420 includes a connecting frame 420a located at the bottom of the second mounting plate 310, a trigger plate 420b hinged on the connecting frame 420a, a trigger switch 420c located on the side wall of the connecting frame 420a and corresponding to the trigger plate 420b, and an eccentric wheel 420d located on the side wall of the rotating main shaft 320a and corresponding to the bottom of the trigger plate 420b. The trigger switch 420c is connected to the vortex tube 410. The connecting frame 420a is used to facilitate the hinged trigger plate 420b. The trigger plate 420b is used to trigger the trigger switch 420c after flipping. The trigger switch 420c is used to trigger the vortex tube 410 to be powered on and start working after being triggered by the trigger plate 420b. The eccentric wheel 420d is used to squeeze one end of the trigger plate 420b to flip it when the rotating main shaft 320a rotates and drives it to rotate.

[0040] In this embodiment, the specific working process is as follows: when the rotating main shaft 320a rotates and drives the eccentric wheel 420d to rotate, the trigger plate 420b is squeezed to flip, and when the trigger plate 420b flips and contacts the trigger switch 420c, the trigger switch 420c triggers the vortex tube 410 to be powered on and start working. When the vortex tube 410 is working, the hot air flow is introduced into the guide tube 410a, and the hot air flow is blown to the inside of the culture dish 120 through the guide tube 410a to quickly dry the moisture in the bacterial solution, thereby preventing the bacteria from flowing randomly in the bacterial solution and forming stacking, which leads to uneven distribution of the bacteria and affects the coating effect.

[0041] Although the present invention has been described above with reference to the embodiments, various modifications may be made thereto and parts thereof may be replaced by equivalents without departing from the scope of the present invention. In particular, as long as there is no structural conflict, the various features in the embodiments disclosed in the present invention may be used in combination with each other in any manner, and the fact that these combinations are not exhaustively described in this specification is only for the sake of omitting space and saving resources. Therefore, the present invention is not limited to the specific embodiments disclosed herein, but includes all technical solutions falling within the scope of the claims.

Claims

1. An automatic microbial coating device, characterized in that: include: A base (100) having a mounting frame (110) and a culture dish (120) on the top thereof; a dilution liquid supply mechanism (200) mounted on the mounting frame (110), wherein the dilution liquid supply mechanism (200) automatically performs a sealed gradient dilution on the bacterial liquid when in operation and then delivers the gradient diluted bacterial liquid to the culture dish (120); a coating mechanism (300) mounted on the mounting frame (110) and corresponding to the culture dish (120), wherein when the dilution liquid supply mechanism (200) transports bacterial liquid into the culture dish (120), the coating mechanism (300) is automatically driven to start working, and a uniform coating operation is performed on the bacterial liquid in the culture dish (120); A drying mechanism (400) is installed on the coating mechanism (300), wherein when the coating mechanism (300) is in operation, the drying mechanism (400) is automatically driven to start working, thereby drying the bacterial liquid in the culture dish (120).

2. An automatic microbial coating device according to claim 1, characterized in that: The dilution and liquid supply mechanism (200) comprises a gradient dilution component (210) and a liquid supply drive component (220) connected to the output end of the gradient dilution component (210) and driving the coating mechanism (300) to work when in operation.

3. An automatic microbial coating device according to claim 2, characterized in that: The gradient dilution assembly (210) comprises a main liquid storage tank (210a) mounted on the mounting frame (110), a connecting tube (210b) detachably connected to an output end of the main liquid storage tank (210a) and having a hydrophobic filter membrane inside, and a buffer tank (210c) having an input end detachably connected to the other end of the connecting tube (210b) and having a liquid outlet (210c-1) at the bottom.

4. An automatic microbial coating device according to claim 3, characterized in that: The liquid supply drive assembly (220) comprises a first mounting plate (220a) located on the mounting frame (110), a swinging member (220b) corresponding to the liquid outlet (210c-1), and a driving member (220c) mounted on one side of the first mounting plate (220a) and having one end drivingly connected to the swinging member (220b) and the other end drivingly connected to the coating mechanism (300).

5. An automatic microbial coating device according to claim 4, characterized in that: The swing member (220b) comprises an eccentric pendulum (220b-1) corresponding to the liquid outlet (210c-1) and a swing block (220b-2) located at one end of the eccentric pendulum (220b-1); The driving member (220c) comprises a ratchet disc (220c-1) rotatably mounted on the side wall of the first mounting plate (220a) and connected to a rotating shaft with a first torsion spring, a driving ratchet (220c-2) mounted on one side of the first mounting plate (220a), a first bevel gear set (220c-3) connected at one end to the driving ratchet (220c-2) via a rotating shaft, and a pulley set (220c-4) connected at one end to an output end of the first bevel gear set (220c-3) via a pulley transmission, and one end of the swinging member (220b) is connected to the output end of the first bevel gear set (220c-3) via a pulley transmission. The inner wall of the ratchet disc (220c-1) is rotatably connected and a second torsion spring is provided on the connected rotating shaft; the swing block (220b-2) corresponds to the elastic pawl on the inner side of the ratchet disc (220c-1); the outer wall of the ratchet disc (220c-1) has a plurality of first saw teeth (220c-11) and a pressing block (220c-12) is provided on a side adjacent to the first saw teeth (220c-11); the outer wall of the ratchet disc (220c-1) has second saw teeth (220c-13) corresponding to the elastic pawl of the driving ratchet (220c-2); The side wall of the first mounting plate (220a) has an escapement (220a-1), the escapement (220a-1) comprising a first mounting seat (220a-11) located on the side wall of the first mounting plate (220a) and having a first limiting sliding groove on the inner wall, an elastic limiting pawl (220a-12) located in the first limiting sliding groove and corresponding to the first sawtooth (220c-11), and an inclined block (220a-13) located at the bottom of the elastic limiting pawl (220a-12) and corresponding to the extrusion block (220c-12), the side wall of the elastic limiting pawl (220a-12) having a first elastic member with the other end connected to the inner wall of the first mounting seat (220a-11).

6. An automatic microbial coating device according to claim 5, characterized in that: The coating mechanism (300) comprises a second mounting plate (310) mounted on the mounting frame (110), a coating assembly (320) located on the first mounting plate (220a), and a first transmission assembly having one end drivingly connected to the driving member (220c) and the other end drivingly connected to the coating assembly (320).

7. An automatic microbial coating device according to claim 6, characterized in that: The coating assembly (320) comprises a rotating spindle (320a) located at the bottom of the second mounting plate (310), a fixing plate (320b) located at the bottom of the rotating spindle (320a), and a coating rod (320c) having a plurality of second elastic members (320c-1) at the top, wherein the other end of the second elastic member (320c-1) is connected to the bottom of the fixing plate (320b); The first transmission assembly comprises a second bevel gear set located on the top of the second mounting plate (310) and connected to the rotating main shaft (320a) at one end, and a transmission pulley connected to the other end of the second bevel gear set at one end and connected to the pulley set (220c-4) via a belt.

8. An automatic microorganism coating device according to claim 7, characterized in that: The bottom of the fixed plate (320b) is provided with an air guide groove (320b-1), the top of the fixed plate (320b) is provided with a second limiting sliding groove, the top of the fixed plate (320b) is provided with a connecting tube (320b-3) one end of which is connected to the air guide groove (320b-1), and the top of the fixed plate (320b) is provided with a counterweight bar (320b-2) extending into the second limiting sliding groove and slidably connected to the inner wall of the connecting tube (320b-3); The top of the coating rod (320c) is provided with an insertion column (320c-2) corresponding to the air guide groove (320b-1).

9. An automatic microbial coating device according to claim 8, characterized in that: The end of the counterweight bar (320b-2) is provided with a sawtooth block (320b-21); The bottom of the buffer bin (210c) is provided with an on-off assembly (210c-2) for opening and closing the liquid outlet (210c-1), the on-off assembly (210c-2) comprising a second mounting seat (210c-21) located at the bottom of the buffer bin (210c) and having a third limiting sliding groove on its inner wall, a magnetic attraction plate (210c-22) located at one side of the second mounting seat (210c-21), and a partition plate (210c-23) extending into the third limiting sliding groove and having a limiting hole (210c-231) at the bottom; A trigger assembly (310a) is mounted on the second mounting plate (310), the trigger assembly (310a) comprising a transmission gear (310a-1) located at the bottom of the second mounting plate (310) and meshing with the sawtooth block (320b-21), a threaded rod (310a-2) located at the top of the second mounting plate (310) and connected to the transmission gear (310a-1) via a rotating shaft, and a trigger frame (310a-3) having one end threadedly sleeved on the threaded rod (310a-2) and slidably sleeved on a limiting sliding rod at the top of the second mounting plate (310), the trigger frame (310a-3) having a limiting protrusion (310a-31) corresponding to the limiting hole (210c-231) at the top.

10. The automatic microorganism coating device according to claim 7, characterized in that: The drying mechanism (400) comprises a vortex tube (410) mounted on the second mounting plate (310) and having a guide tube (410a) at the output end, and a second transmission assembly (420) having one end drivingly connected to the rotating main shaft (320a) and the other end drivingly connected to the vortex tube (410), wherein the reverse direction of the guide tube (410a) forms an angle of thirty degrees with the coating direction of the coating rod (320c); The second transmission assembly (420) comprises a connecting frame (420a) located at the bottom of the second mounting plate (310), a trigger plate (420b) hinged on the connecting frame (420a), a trigger switch (420c) located on the side wall of the connecting frame (420a) and corresponding to the trigger plate (420b), and an eccentric wheel (420d) located on the side wall of the rotating main shaft (320a) and corresponding to the bottom of the trigger plate (420b), wherein the trigger switch (420c) is electrically connected to the vortex tube (410).

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