A rapid extraction device for cell collection and method of use thereof

Abstract

The application discloses a kind of quick extraction equipment and its use method for cell collection, belong to cell collection extraction technical field, by sample is extracted into turnover drum, the elastic force of second spring supports sliding plate, sealing seat can be closely combined with conical groove, by automatic plugging to liquid inlet pipe to prevent sample backflow, piston column moves downwards to make sample can pass one-way valve into catheter, one-way valve can prevent sample backflow to turnover drum, multiple one-way valves are communicated with turnover drum, in the process of piston column depression, multiple centrifugal tubes can be filled simultaneously, and sample inside turnover drum is shunted by multiple catheters, to facilitate completion quantitative sampling work, so the total amount of sample is collected by one-time, and then sample is shunted to multiple centrifugal tubes, to facilitate completion quantitative sampling extraction work, and sample inside turnover drum will not appear backflow condition, to prevent cross contamination.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of cell collection and extraction, in particular to a rapid extraction device for cell collection and a method of use thereof. BACKGROUND

[0002] Hematological tumors are malignant clonal proliferative diseases formed due to blocked differentiation and development of hematopoietic stem cells, including acute and chronic leukemia, lymphoma and multiple myeloma, etc. The pathogenesis and clinical characteristics thereof have strong heterogeneity, and the prognosis also has great difference. Therefore, the clinical diagnosis and treatment thereof needs to integrate a series of clinical data; and the acquisition of the clinical data mainly relies on MICM integrated diagnosis; the MICM integrated diagnosis means that morphological and cytochemical, immunological, cell chromosome karyotype analysis and molecular biology should be used for comprehensive diagnosis of hematological tumors.

[0003] Among them, the cell chromosome karyotype analysis is to reveal whether there is an abnormality in the chromosome by cell culture, microscopic examination and analysis of the number, shape and structure of the cell chromosome, so as to collect and extract cells in various hematological tumor samples. A centrifuge is used to separate supernatant and lower layer cells, the centrifuge tubes containing samples are placed in the centrifuge one by one and inclinedly placed in the centrifuge groove, which helps the sample particles to slide to the bottom along the wall of the centrifuge tube to form a precipitate. The inclined placing and taking operation of the batch centrifuge tubes is not convenient and increases the work burden. Moreover, in the process of placing the hematological tumor sample in multiple centrifuge tubes, a small amount of sample needs to be collected and extracted for multiple times, so that the sample is determined for multiple items. The straight suction type is difficult to accurately control the differences in the sampling pipette, so that the collected sample needs to be sucked for multiple times to complete the quantitative sampling work. Moreover, the sample in the pipette will appear backflow, which is easy to cause cross contamination. SUMMARY

[0004] The present application aims to provide a rapid extraction device for cell collection and a method of use thereof, so as to solve the problems of inconvenient inclined placing and taking operation of the batch centrifuge tubes, increasing work burden; and in the process of placing the hematological tumor sample in multiple centrifuge tubes, a small amount of sample needs to be collected and extracted for multiple times, so that the sample is determined for multiple items. The straight suction type is difficult to accurately control the differences in the sampling pipette, so that the collected sample needs to be sucked for multiple times to complete the quantitative sampling work. Moreover, the sample in the pipette will appear backflow, which is easy to cause cross contamination.

[0005] To achieve the above-mentioned purpose, the present application provides the following technical scheme:

[0006] The utility model provides a kind of quick extraction equipment for cell collection, including centrifuge shell, the centrifuge shell is installed with turnover drum, the bottom of the turnover drum is fixedly connected with bottom support component, the bottom of the centrifuge shell is opened with placing groove, the bottom of bottom support component is installed in placing groove, suction assembly is installed in the turnover drum, the outside of bottom support component is installed with several erection components, the centrifuge tube is equipped in the erection component, the top of the centrifuge tube is clamped with end cover, the end cover is connected with liquid guide component, the top of the liquid guide component is communicated with the bottom of turnover drum, the bottom of the center of turnover drum is installed with liquid inlet pipe, the bottom end of the liquid inlet pipe is connected with adapter, the bottom of the adapter is connected with extension pipe, the inner wall of the liquid inlet pipe is opened with taper slot, the isolation component is installed in the taper slot, the outer of the isolation component is equipped with baffle cover, the baffle cover is fixed in the bottom of the inner wall of turnover drum, the outer wall of the baffle cover is opened with several discharge holes.

[0007] As a further scheme of the utility model, the turnover drum is externally installed with a bearing seat, the bearing seat is clamped with a support plate, the support plate is clamped at the top of the centrifuge shell, two bolts are threadedly connected at the two sides of the support plate, and the support plate is fixed to one side of the centrifuge shell through the bolts, and the outer wall of the turnover drum is provided with a scale bar.

[0008] As a further scheme of the utility model, the suction assembly includes a piston column, the piston column is located in the turnover drum, a groove is formed in the bottom of the piston column corresponding to the position of the baffle cover, a pull rod is provided at the top of the piston column, the pull rod is connected to the bottom of the turnover drum through a limiting sleeve, and a handle is fixed to the top end of the pull rod.

[0009] As a further scheme of the utility model, the bottom support component includes a mounting base, a rectangular slot is formed in the bottom of the mounting base, an operating hole is formed in the top of the inner wall of the rectangular slot, the bottom of the mounting base is connected to the bottom of the inner wall of the placing groove, a support frame is fixed to the top of the mounting base, the support frame is fixed to the bottom of the turnover drum, an opening is formed in the support frame corresponding to the position of the erection component, a hinge seat is mounted at the top of the inner wall of the opening, and the top end of the erection component is hinged to the support frame through the hinge seat.

[0010] As a further scheme of the utility model, the erection component includes a fixed frame, the fixed frame is designed in L shape, two partition strips are fixed to the inner wall of the fixed frame, the bottom end of the centrifuge tube penetrates through the two partition strips and is clamped to the bottom of the inner wall of the fixed frame, a stand is fixed to the bottom of the inner wall of the fixed frame, the top end of the stand penetrates through the two partition strips and is connected to the hinge seat, a telescopic rod is hinged to the top end of the fixed frame through a pin shaft, the top end of the telescopic rod is hinged to the bottom of the turnover drum through a pin shaft, and a first spring is sleeved to the telescopic rod.

[0011] As a further scheme of the present application, the liquid guiding assembly comprises a catheter, a one-way valve is installed at the top end of the catheter, the one-way valve is communicated with the bottom of the turnover drum, a stop valve is connected at the bottom end of the catheter, and the bottom end of the stop valve is inserted into the top of the end cover.

[0012] As a further scheme of the present application, the isolation assembly comprises a sealing seat which is designed in a conical shape and is clamped in a conical groove, a connecting column is fixed at the top of the sealing seat, a sliding plate is fixed at the top end of the connecting column and penetrates through the bottom of the blocking cover, a sliding column is fixed on the sliding plate, the sliding column penetrates through the blocking cover and slides thereon, a second spring is sleeved on the sliding column, and the two ends of the second spring are fixed on the sliding plate and the top of the inner wall of the blocking cover, respectively.

[0013] A method for using a rapid extraction device for cell collection, the method comprising the steps of:

[0014] When the cells in the blood tumor sample are collected and extracted, the bolts on both sides of the support plate are loosened, the locking state between the support plate and the centrifuge shell is released, the support plate is lifted, the turnover drum and the bottom support assembly are taken out of the centrifuge shell, the extension pipe is connected to the bottom end of the liquid inlet pipe through the adapter, the bottom end of the extension pipe is then inserted into the blood tumor sample, the pull handle is pulled upwards, the pull handle drives the piston column to move upwards through the pull rod, the bottom of the turnover drum and the piston column are in a sealed state, the volume of the gas in the turnover drum changes, the pressure of the gas changes, the sliding column is subjected to an upward thrust, the sliding column drives the sliding plate to move upwards inside the blocking cover, the sliding plate drives the sealing seat to move upwards through the connecting column, the conical sealing seat can gradually move away from the conical groove in the liquid inlet pipe, and a gap exists between the conical groove and the sealing seat.

[0015] The total amount of extraction is controlled by observing the scale bar on the outer wall of the turnover drum, the pull handle is stopped when the extraction of the sample is completed, the sliding plate is supported by the elastic force of the second spring, the sliding plate drives the sliding column and the connecting column to move downwards, the connecting column drives the sealing seat to move downwards and be located in the conical groove, the sealing seat is designed in a conical shape and can be tightly fitted with the conical groove, and the liquid inlet pipe is automatically blocked to prevent the sample in the turnover drum from flowing back to the liquid inlet pipe.

[0016] When the sample in the turnover drum is introduced into multiple centrifuge tubes, the handle is pressed down to drive the pull rod and the piston column to move downward, the piston column moves downward to increase the pressure of the inner wall of the turnover drum, the slide column can be continuously pressed down, the lower sealing seat can be tightly fitted with the conical groove, and the effect of preventing backflow is improved, the sample in the turnover drum can enter the conduit through the one-way valve on the conduit and the bottom of the turnover drum, the one-way valve can prevent the sample from flowing back into the turnover drum, the sample can enter the inside of the centrifuge tube with the conduit and the stop valve, multiple one-way valves are communicated with the turnover drum, during the pressing process of the piston column, multiple centrifuge tubes can be filled at the same time, and the sample in the turnover drum is divided by multiple conduits to facilitate quantitative sampling work, and the stop valve at the bottom of the conduit can be closed or opened to fill different numbers of centrifuge tubes as required.

[0017] When the sample in the centrifuge tube is centrifuged to separate the supernatant and the lower layer cells, the extension pipe is detached from the adapter, the bottom support assembly is placed in the placement groove in the centrifuge shell through the support plate, the mounting base in the bottom support assembly is in contact with the bottom of the inner wall of the placement groove, the rectangular groove at the bottom of the mounting base can be embedded on the centrifuge shell structure arranged at the bottom of the placement groove, and the mounting base is limited, at this time, the support plate is lapped on both sides of the top of the centrifuge shell, and the support plate is fixed on the centrifuge shell by tightening the bolts.

[0018] The centrifuge shell is controlled to work to drive the bottom support assembly to rotate, and the bottom support assembly drives the turnover drum and the multiple centrifuge tubes to work, the fixed frame is driven to rotate around the hinge seat under the centrifugal effect, the multiple fixed frames drive the multiple centrifuge tubes to expand, and the centrifuge tubes are in an inclined state, which helps the sample particles to slide to the bottom along the wall of the centrifuge tube to form a precipitate, and the top end of the fixed frame can pull the telescopic rod, and the bottom end of the telescopic rod can rotate around the top of the fixed frame, when the centrifuging work is completed, the telescopic rod is retracted and the fixed frame is deflected by the pulling action of the first spring, the fixed frame drives the centrifuge tubes to return to the initial position, when the centrifuge tube is removed, the stop valve at the end of the conduit is pulled out, the stop valve is separated from the end cover, and then the centrifuge tube is pulled out upward to be removed from the bracket assembly, so that the cell extraction work is facilitated.

[0019] Compared with the prior art, the beneficial effects of the present application are:

[0020] 1. The application is characterized in that the handle is pulled upward to drive the piston column upward through the pull rod, the bottom of the rotating cylinder and the piston column are in a sealed state, the volume of the gas in the rotating cylinder changes, the extension pipe can guide the sample into the liquid inlet pipe through the adapter, the sample is guided into the rotating cylinder through the gap between the tapered groove and the sealing seat and the discharge hole, the total amount of extraction is controlled by observing the scale on the outer wall of the rotating cylinder, the handle is pulled to stop the extraction of the sample, the slide plate is supported by the elastic force of the second spring, the slide plate drives the slide column and the connecting column to move downward, the connecting column drives the sealing seat to move downward and is located in the tapered groove, the sealing seat is designed in a tapered shape to tightly fit the tapered groove, the liquid inlet pipe is automatically blocked to prevent the sample in the rotating cylinder from flowing back to the liquid inlet pipe, the handle is pressed downward to drive the pull rod and the piston column to move downward, the sample in the rotating cylinder can enter the conduit through the one-way valve, the one-way valve can prevent the sample from flowing back to the rotating cylinder, the sample can enter the centrifugal tube through the conduit and the stop valve, the plurality of one-way valves are in communication with the rotating cylinder, the plurality of centrifugal tubes can be filled simultaneously during the downward movement of the piston column, and the sample in the rotating cylinder is divided by the plurality of conduits to complete the quantitative sampling work, the stop valve at the bottom of the conduit can be closed or opened to fill different numbers of centrifugal tubes as needed, so that the total amount of sample is collected at one time and then divided into a plurality of centrifugal tubes to quickly complete the quantitative sampling work, and the sample in the rotating cylinder will not backflow to prevent cross contamination.

[0021] 2. The application is characterized in that the centrifuge shell is controlled to work to drive the bottom support assembly to rotate, the bottom support assembly drives the rotating cylinder and the plurality of centrifugal tubes to work, the fixed frame is driven by the centrifugal force to rotate around the hinge seat, the plurality of fixed frames drive the plurality of centrifugal tubes to expand, and the centrifugal tubes are in an inclined state, which helps the sample particles to slide along the wall of the centrifugal tube to the bottom to form a precipitate, and the top end of the fixed frame can pull the telescopic rod, so that the bottom end of the telescopic rod can rotate around the top of the fixed frame, when the centrifugal work is completed, the telescopic rod is retracted and the fixed frame is deflected by the pulling action of the first spring, so that the fixed frame drives the centrifugal tube to return to the initial position, at this time the centrifugal tube is in a relatively vertical state, so it will not interfere with the taking and placing work of the centrifugal tube, when the centrifugal tube is taken down, the stop valve at the end of the conduit is pulled out to make the stop valve separate from the end cover, and then the centrifugal tube is pulled out upward to be taken out from the erecting assembly, improving the work efficiency and operation convenience. BRIEF DESCRIPTION OF DRAWINGS

[0022] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings needed for the description of the embodiments will be briefly introduced as follows. Obviously, the drawings in the following description are only some embodiments of the present application, and for those skilled in the art, other drawings can also be obtained from these drawings without any creative effort.

[0023] Figure 1 A schematic structural view of the present application;

[0024] Figure 2 A schematic structural view of the present application;

[0025] Figure 3 A schematic structural view of the present application;

[0026] Figure 4 A schematic structural view of the present application;

[0027] Figure 5 A schematic structural view of the present application;

[0028] Figure 6 A schematic structural view of the present application Figure 5 A schematic structural view of the present application

[0029] Figure 7 A schematic structural view of the present application

[0030] Figure 8 A schematic structural view of the present application.

[0031] In the drawings, the components represented by the respective reference numerals are listed as follows:

[0032] 1, centrifuge housing; 2, placement groove; 3, support plate; 4, bolt; 5, bearing seat; 6, turnover drum; 7, scale bar; 8, suction assembly; 801, piston column; 802, groove; 803, pull rod; 804, handle; 9, bottom support assembly; 901, mounting base; 902, rectangular groove; 903, operation hole; 904, support frame; 905, opening; 906, hinged seat; 10, erecting assembly; 101, fixing frame; 102, partition bar; 103, stand column; 104, telescopic rod; 105, first spring; 11, centrifugal tube; 12, liquid guide assembly; 121, guide pipe; 122, one-way valve; 123, flow-stopping valve; 13, end cover; 14, liquid inlet pipe; 15, adapter; 16, extension pipe; 17, conical groove; 18, isolation assembly; 181, sealing seat; 182, connecting column; 183, sliding plate; 184, sliding column; 185, second spring; 19, baffle cover; 20, discharge hole. DETAILED DESCRIPTION

[0033] Clearly, the embodiments described are only a part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments of the present application, all other embodiments obtained by a person of ordinary skill in the art without creative effort belong to the scope of protection of the present application.

[0034] Please refer to Figures 1-8 The present application provides a technical solution:

[0035] A kind of fast extraction equipment for cell collection, including centrifuge shell 1, centrifuge shell 1 is installed with turnover drum 6, the bottom of turnover drum 6 is fixedly connected with bottom support assembly 9, the bottom of centrifuge shell 1 is opened with placing groove 2, bottom support assembly 9 is installed in placing groove 2, bottom support assembly 9 includes installation base 901, the bottom of installation base 901 is opened with rectangular groove 902, the top of the inner wall of rectangular groove 902 is opened with operation hole 903, the bottom of installation base 901 is connected with the bottom of the inner wall of placing groove 2, the top of installation base 901 is fixed with support frame 904, support frame 904 is fixed with the bottom of turnover drum 6.

[0036] As a further scheme of the present application, bearing seat 5 is installed outside turnover drum 6, bearing seat 5 is clamped with support plate 3, support plate 3 is clamped at the top of centrifuge shell 1, two bolts 4 are respectively screwed on the two sides of support plate 3, and support plate 3 is fixed with one side of centrifuge shell 1 by bolt 4, the bolt 4 on the two sides of support plate 3 is loosened, the locking state between support plate 3 and centrifuge shell 1 is released, support plate 3 is lifted, and then turnover drum 6 and bottom support assembly 9 are conveniently taken out of centrifuge shell 1. The outer wall of turnover drum 6 is provided with scale bar 7, and the total amount of extraction is controlled by observing the scale bar 7 on the outer wall of turnover drum 6. When working, bottom support assembly 9 is placed in placing groove 2 in centrifuge shell 1 by support plate 3, installation base 901 in bottom support assembly 9 contacts with the bottom of the inner wall of placing groove 2, rectangular groove 902 in the bottom of installation base 901 can be embedded on the centrifuge shell structure installed on the inner bottom of placing groove 2, to limit installation base 901, improve the stability of centrifugal work.

[0037] The bottom support assembly 9 is externally mounted with several erecting assemblies 10, the erecting assemblies 10 are internally provided with centrifugal tubes 11, the top of the centrifugal tube 11 is clamped with an end cover 13, the end cover 13 is communicated with a liquid guide assembly 12, the top end of the liquid guide assembly 12 is communicated with the bottom of the turnover drum 6, the bottom of the turnover drum 6 is internally mounted with a liquid inlet pipe 14, the bottom end of the liquid inlet pipe 14 is connected with an adapter 15, the bottom of the adapter 15 is connected with an extension pipe 16; the inner wall of the liquid inlet pipe 14 is internally provided with a tapered groove 17, the tapered groove 17 is internally mounted with a separation assembly 18, the separation assembly 18 is externally provided with a baffle cover 19, the baffle cover 19 is fixed on the bottom of the inner wall of the turnover drum 6, and the outer wall of the baffle cover 19 is externally provided with a plurality of discharge holes 20; the total amount of the sample is collected at one time, and then the sample is shunted to the plurality of centrifugal tubes 11, so that the quantitative sampling and extraction work is conveniently and quickly completed, and the sample in the turnover drum 6 cannot flow back, thereby preventing cross contamination.

[0038] As a further scheme of the present application, the turnover drum 6 is internally mounted with a suction assembly 8, the suction assembly 8 comprises a piston column 801, the piston column 801 is located in the turnover drum 6, the bottom of the piston column 801 is externally provided with a groove 802 corresponding to the position of the baffle cover 19, the top of the piston column 801 is externally provided with a pull rod 803, the pull rod 803 is connected with the bottom of the turnover drum 6 through a limiting sleeve, and the top end of the pull rod 803 is fixedly provided with a handle 804. The handle 804 is pressed downward to drive the pull rod 803 and the piston column 801 to move downward, the pressure of the inner wall of the turnover drum 6 is increased in the process of the piston column 801 moving downward, the groove 802 at the bottom of the piston column 801 can be moved to the outside of the baffle cover 19, and the bottom of the piston column 801 is in contact with the bottom of the inner wall of the turnover drum 6, so that the sample in the turnover drum 6 can be conveniently discharged, and the sample in the turnover drum 6 cannot be completely discharged due to the interference of the baffle cover 19.

[0039] As a further scheme of the present application, the erecting assembly 10 comprises a fixed frame 101, the fixed frame 101 is designed in an L shape, two partition strips 102 are fixedly arranged on the inner wall of the fixed frame 101, the bottom end of the centrifugal tube 11 penetrates through the two partition strips 102 and is clamped with the bottom of the inner wall of the fixed frame 101, a stand column 103 is fixedly arranged on the bottom of the inner wall of the fixed frame 101, the top end of the stand column 103 penetrates through the two partition strips 102 and is connected with the hinged seat 906, the top end of the fixed frame 101 is hingedly connected with a telescopic rod 104 through a pin shaft, the top end of the telescopic rod 104 is hingedly connected with the bottom of the turnover drum 6 through a pin shaft, and the telescopic rod 104 is externally sleeved with a first spring 105.

[0040] The fixed frame 101 can drive the column 103 to rotate around the hinge seat 906 under the centrifugal effect, so that the plurality of fixed frames 101 drive the plurality of centrifugal tubes 11 to expand, so that the centrifugal tubes 11 are in an inclined state, which helps the sample particles to slide along the wall of the centrifugal tube 11 to the bottom to form a precipitate; when the centrifugation is completed, the telescopic rod 104 is retracted and the fixed frame 101 is deflected by the pulling action of the first spring 105, so that the fixed frame 101 drives the centrifugal tube 11 to return to the initial position, and the centrifugal tube 11 is in a relatively vertical state, which facilitates the taking and placing work of the centrifugal tube 11.

[0041] As a further scheme of the application, the liquid guide assembly 12 comprises a guide pipe 121, a one-way valve 122 is installed at the top end of the guide pipe 121, the one-way valve 122 is in communication with the bottom of the turnover drum 6, a stop valve 123 is connected at the bottom end of the guide pipe 121, and the bottom end of the stop valve 123 is inserted into the top of the end cover 13.

[0042] Because the one-way valve 122 on the guide pipe 121 is in communication with the bottom of the turnover drum 6, the sample in the turnover drum 6 can enter the guide pipe 121 through the one-way valve 122, the one-way valve 122 can prevent the sample from flowing back into the turnover drum 6, and the sample can enter the inside of the centrifugal tube 11 along with the guide pipe 121 and the stop valve 123; the stop valve 123 can be closed or opened, so that different numbers of centrifugal tubes 11 can be filled as needed.

[0043] As a further scheme of the application, the isolation assembly 18 comprises a sealing seat 181, the sealing seat 181 is designed in a conical shape and is clamped in the conical groove 17, a connecting column 182 is fixed at the top of the sealing seat 181, a sliding plate 183 is fixed at the top end of the connecting column 182 and penetrates through the bottom of the blocking cover 19, a sliding column 184 is fixed on the sliding plate 183, the sliding column 184 penetrates through and slides on the blocking cover 19, a second spring 185 is sleeved on the sliding column 184, and the two ends of the second spring 185 are fixed on the sliding plate 183 and the top of the inner wall of the blocking cover 19 respectively.

[0044] The sliding plate 183 is supported by the elastic force of the second spring 185, so that the sliding plate 183 drives the sliding column 184 and the connecting column 182 to move downward, and the connecting column 182 drives the sealing seat 181 to move downward and be located in the conical groove 17; because the sealing seat 181 is designed in a conical shape, it can be tightly fitted with the conical groove 17, so that the liquid inlet pipe 14 is automatically blocked to prevent the sample in the turnover drum 6 from flowing back into the liquid inlet pipe 14.

[0045] A use method of a rapid extraction device for cell collection, the use method comprising the following steps:

[0046] When the cells in the blood tumor sample are collected and extracted, the bolts 4 on both sides of the support plate 3 are loosened, the locking state between the support plate 3 and the centrifuge shell 1 is released, the support plate 3 is lifted, and the turnover drum 6 and the bottom support assembly 9 are taken out of the centrifuge shell 1, the extension pipe 16 is connected to the bottom end of the liquid inlet pipe 14 through the adapter 15, and then the bottom end of the extension pipe 16 is inserted into the blood tumor sample, the pull handle 804 is pulled upward, the pull handle 804 drives the piston column 801 to move upward through the pull rod 803, the bottom of the turnover drum 6 is in a sealed state with the piston column 801, the volume of the gas in the turnover drum 6 changes, which causes the change of the gas pressure, the slide column 184 is subjected to an upward thrust, and the slide plate 183 moves upward in the baffle cover 19, the sealing seat 181 is driven to move upward by the connecting column 182, the conical sealing seat 181 can gradually move away from the conical groove 17 in the liquid inlet pipe 14, and there is a gap between the conical groove 17 and the sealing seat 181, the bottom end of the extension pipe 16 is inserted into the blood tumor sample, so that the sample can be introduced into the liquid inlet pipe 14 through the adapter 15, and then the sample is introduced into the turnover drum 6 through the gap between the conical groove 17 and the sealing seat 181 and the discharge hole 20;

[0047] The total amount of extraction is controlled by observing the scale bar 7 on the outer wall of the turnover drum 6, the pull handle 804 is stopped when the extraction of the sample is completed, the slide plate 183 is supported by the elastic force of the second spring 185, the slide plate 183 drives the slide column 184 and the connecting column 182 to move downward, the connecting column 182 drives the sealing seat 181 to move downward and be located in the conical groove 17, the sealing seat 181 is designed in a conical shape, which can be tightly fitted with the conical groove 17, and the liquid inlet pipe 14 is automatically blocked to prevent the sample in the turnover drum 6 from flowing back to the liquid inlet pipe 14;

[0048] When the sample in the rotating drum 6 is introduced into the plurality of centrifugal tubes 11, the handle 804 is pressed downward to drive the pull rod 803 and the piston column 801 to move downward, and the piston column 801 can increase the pressure of the inner wall of the rotating drum 6 during the downward movement, so that the slide column 184 can be continuously pressed downward, and the lower sealing seat 181 can be tightly fitted with the conical groove 17, thereby improving the anti-backflow effect. Because the one-way valve 122 on the conduit 121 communicates with the bottom of the rotating drum 6, the sample in the rotating drum 6 can enter the conduit 121 through the one-way valve 122, and the one-way valve 122 can prevent the sample from flowing back into the rotating drum 6. The sample can enter the inside of the centrifugal tube 11 along with the conduit 121 and the stop valve 123. A plurality of one-way valves 122 are in communication with the rotating drum 6. During the downward movement of the piston column 801, a plurality of centrifugal tubes 11 can be filled at the same time. Moreover, the sample in the rotating drum 6 is divided by a plurality of conduits 121 to facilitate quantitative sampling. The stop valve 123 at the bottom of the conduit 121 can be closed or opened, and different numbers of centrifugal tubes 11 can be filled as needed.

[0049] When the sample in the centrifugal tube 11 is centrifuged to separate the supernatant and the lower layer of cells, the extension pipe 16 is detached from the adapter 15, and the bottom support assembly 9 is placed in the placement groove 2 in the centrifuge shell 1 through the support plate 3. The mounting base 901 in the bottom support assembly 9 is in contact with the bottom of the inner wall of the placement groove 2. The rectangular groove 902 at the bottom of the mounting base 901 can be embedded in the centrifuge shell structure installed at the bottom of the placement groove 2, thereby limiting the mounting base 901. At this time, the support plate 3 is lapped on both sides of the top of the centrifuge shell 1, and the support plate 3 is fixed on the centrifuge shell 1 by tightening the bolt 4.

[0050] The centrifuge shell 1 is controlled to work to drive the bottom support assembly 9 to rotate, and the bottom support assembly 9 drives the rotating drum 6 and the plurality of centrifugal tubes 11 to work. The fixed frame 101 can drive the vertical column 103 to rotate around the hinge seat 906 under the centrifugal force, so that the plurality of fixed frames 101 drive the plurality of centrifugal tubes 11 to expand, so that the centrifugal tube 11 is in an inclined state, which helps the sample particles to slide along the wall of the centrifugal tube 11 to the bottom to form a precipitate. At the same time, the top end of the fixed frame 101 can pull the telescopic rod 104, so that the bottom end of the telescopic rod 104 can rotate around the top of the fixed frame 101. When the centrifuging work is completed, the telescopic rod 104 is retracted and the fixed frame 101 is deflected by the pulling action of the first spring 105, so that the fixed frame 101 drives the centrifugal tube 11 to return to the initial position. When the centrifugal tube 11 is removed, the stop valve 123 at the end of the conduit 121 is pulled out, so that the stop valve 123 is separated from the end cover 13. Then, the centrifugal tube 11 can be taken out from the erecting assembly 10 by pulling it upward, so as to facilitate the extraction of cells.

Claims

1. A rapid extraction device for cell collection comprising a centrifuge housing (1), characterized in that: The centrifuge shell (1) is provided with a turnover drum (6), the bottom of the turnover drum (6) is fixedly connected with a bottom support assembly (9), the bottom of the centrifuge shell (1) is provided with a placing groove (2), the bottom of the bottom support assembly (9) is arranged in the placing groove (2), a suction assembly (8) is arranged in the turnover drum (6), a plurality of erecting assemblies (10) are arranged on the outer portion of the bottom support assembly (9), a centrifuge tube (11) is arranged in the erecting assembly (10), an end cover (13) is connected to the top of the centrifuge tube (11), a liquid guide assembly (12) is arranged on the end cover (13), the top of the liquid guide assembly (12) is communicated with the bottom of the turnover drum (6), a liquid inlet pipe (14) is arranged at the center of the bottom of the turnover drum (6), the bottom of the liquid inlet pipe (14) is connected with an adapter (15), the bottom of the adapter (15) is connected with an extension pipe (16), a tapered groove (17) is arranged on the inner wall of the liquid inlet pipe (14), an isolation assembly (18) is arranged in the tapered groove (17), a baffle cover (19) is arranged on the outer portion of the isolation assembly (18), the baffle cover (19) is fixedly arranged on the bottom of the inner wall of the turnover drum (6), a plurality of discharge holes (20) are arranged on the outer wall of the baffle cover (19). The suction assembly (8) comprises a piston column (801), the piston column (801) is arranged in the turnover drum (6), a recess (802) is arranged on the bottom of the piston column (801) corresponding to the position of the baffle cover (19), a pull rod (803) is arranged on the top of the piston column (801), the pull rod (803) is connected with the bottom of the turnover drum (6) through a limiting sleeve, and a handle (804) is fixedly arranged on the top of the pull rod (803). The bottom support assembly (9) comprises a mounting base (901), a support frame (904) is fixedly arranged on the top of the mounting base (901), and the support frame (904) is fixedly arranged on the bottom of the turnover drum (6). The erecting assembly (10) comprises a fixed frame (101), the fixed frame (101) is designed in an L shape, two partition strips (102) are fixedly arranged on the inner wall of the fixed frame (101), the bottom end of the centrifuge tube (11) penetrates through the two partition strips (102) and is clamped with the bottom of the inner wall of the fixed frame (101), a stand column (103) is fixedly arranged on the bottom of the inner wall of the fixed frame (101), the top end of the stand column (103) penetrates through the two partition strips (102) and is connected with a hinged seat (906), a telescopic rod (104) is hingedly connected to the top end of the fixed frame (101) through a pin shaft, the top end of the telescopic rod (104) is hingedly connected with the bottom of the turnover drum (6) through a pin shaft, and a first spring (105) is arranged on the outer sleeve of the telescopic rod (104).

2. The rapid extraction device for cell collection according to claim 1, characterized by: The outer wall of the turnover drum (6) is provided with a scale bar (7).

3. The rapid extraction device for cell collection according to claim 1, characterized by: The bottom of the mounting base (901) is provided with a rectangular groove (902), and the top of the inner wall of the rectangular groove (902) is provided with an operation hole (903). The bottom of the mounting base (901) is connected with the bottom of the inner wall of the placing groove (2). The outer side of the support frame (904) is provided with an opening (905) corresponding to the position of the erecting assembly (10). The top of the inner wall of the opening (905) is provided with a hinged seat (906). The top end of the erecting assembly (10) is hinged with the support frame (904) through the hinged seat (906).

4. The rapid extraction device for cell collection according to claim 1, characterized by: The liquid guiding assembly (12) comprises a conduit (121), and the top end of the conduit (121) is provided with a one-way valve (122). The one-way valve (122) is communicated with the bottom of the turnover drum (6). The bottom end of the conduit (121) is connected with a check valve (123), and the bottom end of the check valve (123) is inserted into the top of the end cover (13).

5. The rapid extraction device for cell collection according to claim 1, characterized in that: The isolation assembly (18) comprises a sealing seat (181), which is tapered and clamped in the tapered groove (17). The top of the sealing seat (181) is fixedly provided with a connecting column (182). The top end of the connecting column (182) penetrates the bottom of the blocking cover (19) and is fixedly provided with a sliding plate (183). The sliding plate (183) is fixedly provided with a sliding column (184), which penetrates and slides on the blocking cover (19). The sliding column (184) is sleeved with a second spring (185), and the two ends of the second spring (185) are fixedly provided on the sliding plate (183) and the top of the inner wall of the blocking cover (19).

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