Fermentation equipment based on microbial agent preparation

Abstract

The invention relates to the technical field of microbial agent preparation, in particular to microbial agent preparation-based fermentation equipment which comprises an equipment shell, a fermentation cavity is formed in the equipment shell, and a shake culture mechanism used for bottom aeration to improve the oxygen dissolving efficiency and a splash-proof auxiliary mechanism are arranged in the fermentation cavity. The equipment shell is provided with an automatic control mechanism for adjusting the growth environment of thalli, the lower end of the equipment shell is provided with an anti-skid damping mechanism for leveling the equipment, and the splash-proof auxiliary mechanism can adjust the aeration rate of the shake culture mechanism by detecting the dissolved oxygen content in the culture bottle. By arranging the shake culture mechanism and utilizing mutual cooperation between the rectangular shaking table and the oxygen supply assembly, vortex can be generated through horizontal circular motion of the rectangular shaking table, oxygen transfer of a gas-liquid interface can be enhanced, microbubbles can be directly injected from the bottom of a culture bottle, oxygen diffusion limitation during high-density culture is broken through, and the culture efficiency is improved. The aeration efficiency and the thallus growth synchronism can be effectively improved.

Description

Technical Field

[0001] The present invention relates to the technical field of microbial agent preparation, and in particular to fermentation equipment based on microbial agent preparation. Background Art

[0002] A shaker (also known as an oscillating incubator) is a commonly used laboratory fermentation equipment in the preparation of microbial agents. It provides dissolved oxygen and mixing conditions through oscillation and is widely used in seed liquid propagation, small-scale trial process optimization and other links. The traditional shaker is mainly composed of an oscillation platform, a temperature control system and a speed regulation device.

[0003] However, conventional shaking incubators in the prior art usually use top aeration to provide dissolved oxygen, which is easily restricted by the gas-liquid interface. The oxygen transfer rate will drop significantly during high-density culture, which can easily cause the bacteria to die due to lack of oxygen and asynchronous growth of the bacteria. Summary of the invention

[0004] In view of the shortcomings of the prior art, the present invention provides a fermentation device based on the preparation of microbial inoculants, which solves the technical problem that the oxygen transfer rate of the shaker in the prior art will be significantly reduced during high-density culture, and has the advantage of being able to effectively improve the aeration efficiency and the synchronization of bacterial growth.

[0005] To solve the above technical problems, the present invention provides the following technical solutions: a fermentation device based on the preparation of microbial inoculants, comprising an equipment shell, a fermentation cavity is opened inside the equipment shell, an oscillation culture mechanism and an anti-splash auxiliary mechanism are arranged inside the fermentation cavity for providing oxygen to the culture solution and thus improving the dissolved oxygen efficiency, an automatic control mechanism for adjusting the bacterial growth environment is arranged on the equipment shell, an anti-skid shock-absorbing mechanism for leveling the equipment is arranged at the lower end of the equipment shell, the anti-skid shock-absorbing mechanism can adjust the aeration rate of the oscillation culture mechanism by detecting the dissolved oxygen content in the culture bottle, the anti-skid shock-absorbing mechanism and the oscillation culture mechanism can cooperate to achieve two-way shock absorption, and after the staff puts the culture bottle into the fermentation cavity, the oscillation culture mechanism and the anti-splash auxiliary mechanism will automatically oscillate and culture the bacteria.

[0006] Preferably, the shaking culture mechanism includes a rectangular shaker movably installed inside the fermentation cavity, an air guide duct is buried inside the rectangular shaker, a plurality of limiting bases are fixedly installed on the rectangular shaker, a culture bottle assembly is detachably installed on the limiting base, the bottom of the culture bottle assembly is recessed inward to form a circular groove, a silicone one-way valve is arranged inside the circular groove, a connecting circular tube is coaxially fixedly installed on the limiting base, and the connecting circular tube is connected to the silicone one-way valve. During the fermentation process of the bacteria, oxygen will enter the interior of the rectangular shaker through the oxygen supply assembly, and then enter the interior of the culture bottle through the connecting circular tube and the silicone one-way valve, thereby realizing bottom aeration.

[0007] Preferably, an oxygen supply assembly is provided on the device housing, and the oxygen supply assembly includes an oxygen valve fixedly mounted on the outside of the device housing, the air inlet end of the oxygen valve is connected to the gas storage tank, the air outlet end of the oxygen valve is connected to an exhaust hose, and the exhaust hose is connected to the air duct. Initially, the oxygen valve is in a closed state. When the oxygen valve is opened, oxygen will enter the interior of the air duct through the exhaust hose.

[0008] Preferably, an exhaust passage for connecting the air guide duct and the connecting circular tube is coaxially provided on the limiting base.

[0009] Preferably, the splash-proof auxiliary mechanism includes a lifting push rod fixedly mounted on the rectangular shaker, a mounting bracket is provided at the upper end of the lifting push rod, a limiting tube cover is fixedly mounted on the mounting bracket, a rubber flange is fixedly mounted on the inner side wall of the limiting tube cover, a triangular bracket is fixedly mounted on the upper end of the limiting tube cover, a DO sensor for detecting dissolved oxygen is coaxially mounted at the lower end of the triangular bracket, a hydrophobic breathable membrane is provided on the triangular bracket, and during the shaking culture process, the lifting push rod will automatically contract downward, so that the limiting tube cover is sleeved over the culture bottle.

[0010] Preferably, the cross-sectional diameter of the limiting tube cover is smaller than the cross-sectional diameter at the culture bottle S, the number of the limiting tube covers matches the number of the limiting bases, and when the limiting tube cover moves downward, the rubber flange will contact the outer side of the culture bottle, thereby further pressing and limiting the culture bottle assembly.

[0011] Preferably, the hydrophobic breathable membrane is a PTFE membrane, which allows gas exchange but prevents water evaporation, and can effectively reduce the evaporation of the culture solution during long-term shaking culture, thereby avoiding the influence on the culture medium concentration and bacterial growth.

[0012] Preferably, the DO sensor is electrically connected to the oxygen supply component via a wire, and a closed-loop feedback system is formed with the oxygen supply component through real-time monitoring data of the DO sensor, thereby accurately adjusting the oxygen flow rate of bottom aeration.

[0013] Preferably, the automatic control mechanism includes a control panel fixedly mounted on the equipment housing, a temperature control component is arranged inside the fermentation cavity, an oscillation drive component for driving the rectangular shaker to perform eccentric circular motion is arranged at the bottom of the equipment housing, a movable cover is rotatably connected to the upper end of the fermentation cavity, a buffer convex strip is fixedly mounted on the inner wall of the fermentation cavity, a silicone handle is fixedly mounted on the outer side of the movable cover, the temperature control component can automatically adjust the temperature and humidity environment inside the fermentation cavity according to the program setting of the staff, and the oscillation drive component is mainly composed of a stepper motor, which can be programmed to accurately control the rotation speed, direction and displacement to realize complex oscillation modes (such as linear / circular compound motion).

[0014] Preferably, the anti-slip shock-absorbing mechanism includes a mounting sleeve fixedly installed at the bottom of the equipment housing, an adjusting screw is movably installed inside the mounting sleeve, an anti-slip silicone pad is fixedly installed at the lower end of the adjusting screw, the anti-slip silicone pad is coaxially arranged with the adjusting screw, and the adjusting screw is threadedly connected to the mounting sleeve.

[0015] By means of the above technical solution, the present invention provides a fermentation device based on the preparation of microbial inoculants, which has at least the following beneficial effects: 1. The present invention sets an oscillating culture mechanism and utilizes the cooperation between the rectangular shaker and the oxygen supply assembly. It can not only generate vortices through the horizontal circular motion of the rectangular shaker to enhance the oxygen transfer at the gas-liquid interface, but also directly inject microbubbles from the bottom of the culture bottle to break through the oxygen diffusion limitation during high-density culture, and effectively improve the aeration efficiency and the synchronization of bacterial growth.

[0016] 2. The present invention realizes rapid sealing installation by setting up a shaking culture mechanism and utilizing the cooperation between the connecting round tube and the silicone one-way valve, which can greatly shorten the operation time and avoid leakage of the gas path, and is easy to use.

[0017] 3. The present invention provides an anti-splash auxiliary mechanism and utilizes the coordinated compression fit between the lifting limit tube cover and the rubber flange to achieve secondary mechanical fixation of the culture bottle, which can effectively prevent the bottle from tipping over or liquid splashing during high-speed shaking and ensure stability during long-term shaking culture.

[0018] 4. The present invention is provided with an anti-splash auxiliary mechanism. The DO sensor is automatically inserted into the culture medium as the tube cover moves downward, dynamically feeds back the dissolved oxygen data and links the oxygen supply component to adjust the aeration volume, which can effectively ensure the dissolved oxygen accuracy. In addition, the hydrophobic breathable membrane covers the bottle mouth, which can effectively reduce the loss of water in the culture medium and avoid the interference of changes in the culture medium concentration on the growth of bacteria.

[0019] 5. The present invention sets up an automatic control mechanism and utilizes the coordinated cooperation of the temperature control component and the oscillation drive component, which can not only accurately control the temperature and humidity environment in the fermentation cavity, but also realize the intelligent switching of various oscillation modes (such as circular / linear compound motion) through a programmable stepper motor, thereby significantly improving the environmental adaptability and process controllability of culturing different strains.

[0020] 6. The present invention provides an anti-skid shock-absorbing mechanism and utilizes the mutual cooperation between the mounting sleeve and the adjusting screw, which can not only realize the rapid leveling of the equipment (single adjustment time is less than 30 seconds), but also effectively eliminate the shaking of the equipment caused by uneven ground (the vibration amplitude is reduced by more than 60%), thereby effectively ensuring the stability during the cultivation process.

[0021] 7. The present invention provides an anti-skid shock-absorbing mechanism and utilizes the synergistic effect of the anti-skid silicone pad and the adjusting screw to provide high friction fixation (static friction coefficient ≥ 0.8) and absorb vibration energy during equipment operation (shock absorption efficiency reaches 45%), thereby significantly extending the service life of the equipment. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] The drawings described herein are used to provide a further understanding of the present application and constitute a part of the present application. The illustrative embodiments of the present application and their descriptions are used to explain the present application and do not constitute an improper limitation on the present application. In the drawings: Figure 1 The three-dimensional structure of the overall structure of the present invention Figure 1 ; Figure 2 The three-dimensional structure of the overall structure of the present invention Figure 2 ; Figure 3 The three-dimensional structure of the overall structure of the present invention Figure 3 ; Figure 4 It is a structural schematic diagram of the shaking culture mechanism in the present invention; Figure 5 It is a schematic diagram of the structure of the limiting base in the present invention; Figure 6 It is a schematic diagram of the structure of the culture bottle assembly in the present invention; Figure 7 It is a structural schematic diagram of the mounting bracket in the present invention; Figure 8 It is a structural schematic diagram of the splash-proof auxiliary mechanism in the present invention; Fig. 9 It is a structural schematic diagram of the position-limiting pipe cover in the present invention; Fig.10 It is a structural schematic diagram of the anti-skid and shock-absorbing mechanism in the present invention.

[0023] In the figure: 1. Equipment shell; 2. Fermentation cavity; 3. Oscillating culture mechanism; 301. Rectangular shaker; 302. Limiting base; 303. Culture bottle assembly; 304. Circular groove; 305. Silicone one-way valve; 306. Connecting round pipe; 307. Oxygen supply assembly; 4. Splash-proof auxiliary mechanism; 401. Lifting push rod; 402. Mounting bracket; 403. Limiting pipe cover; 404. Rubber flange; 405. Triangular bracket; 406. DO sensor; 407. Hydrophobic breathable membrane; 5. Automatic control mechanism; 501. Control panel; 502. Temperature control assembly; 503. Oscillating drive assembly; 504. Movable cover; 505. Buffer convex strip; 506. Silicone handle; 6. Anti-skid shock-absorbing mechanism; 601. Mounting sleeve; 602. Anti-skid silicone pad; 603. Adjusting screw. DETAILED DESCRIPTION

[0024] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0025] Embodiment 1 Traditional shaking incubators in the prior art usually use top aeration to provide dissolved oxygen, which is easily restricted by the gas-liquid interface. The oxygen transfer rate will drop significantly during high-density culture, which can easily cause the cells to die due to lack of oxygen and asynchronous growth of the cells. In order to solve this technical defect in the prior art, Figure 1-Figure 7 As shown, this embodiment proposes a fermentation device based on the preparation of microbial inoculants, which can generate vortexes through the horizontal circular motion of the rectangular shaking table 301 to enhance the oxygen transfer at the gas-liquid interface, and can directly inject microbubbles from the bottom of the culture bottle to break through the oxygen diffusion limitation during high-density culture. The fermentation device includes an equipment shell 1, and a fermentation cavity 2 is opened inside the equipment shell 1. The fermentation cavity 2 is provided with an oscillation culture mechanism 3 and an anti-splash auxiliary mechanism 4 for bottom aeration to improve the dissolved oxygen efficiency. The equipment shell 1 is provided with an automatic control mechanism 5 for adjusting the bacterial growth environment, and the lower end of the equipment shell 1 is provided with an anti-skid shock-absorbing mechanism 6 for equipment leveling. The anti-splash auxiliary mechanism 4 can adjust the aeration rate of the oscillation culture mechanism 3 by detecting the dissolved oxygen content in the culture bottle. The anti-skid shock-absorbing mechanism 6 and the oscillation culture mechanism 3 can cooperate to achieve two-way shock absorption. After the staff puts the culture bottle into the fermentation cavity 2, the oscillation culture mechanism 3 and the anti-splash auxiliary mechanism 4 will automatically perform oscillation culture on the strain.

[0026] Specifically, the shaking culture mechanism 3 includes a rectangular shaking table 301 movably installed inside the fermentation cavity 2, an air guide pipe is buried inside the rectangular shaking table 301, a plurality of limiting bases 302 are fixedly installed on the rectangular shaking table 301, a culture bottle assembly 303 is detachably installed on the limiting base 302, the bottom of the culture bottle assembly 303 is recessed inward to form a circular groove 304, a silicone one-way valve 305 is arranged inside the circular groove 304, a connecting circular pipe 306 is coaxially fixedly installed on the limiting base 302, an exhaust channel for connecting the air guide pipe and the connecting circular pipe 306 is coaxially opened on the limiting base 302, and the connecting circular pipe 303 is connected to the fermentation cavity 2. 06 matches the silicone one-way valve 305, and an oxygen supply component 307 is arranged on the equipment housing 1. During the fermentation process of the bacteria, oxygen will enter the interior of the rectangular shaker 301 through the oxygen supply component 307, and then enter the interior of the culture bottle through the connecting round tube 306 and the silicone one-way valve 305, so as to realize bottom aeration. The oxygen supply component 307 includes an oxygen valve fixedly installed on the outside of the equipment housing 1, the air inlet end of the oxygen valve is connected to the gas storage tank, and the air outlet end of the oxygen valve is connected to an exhaust hose, and the exhaust hose is connected to the air guide pipe. Initially, the oxygen valve is in a closed state. When the oxygen valve is opened, oxygen will enter the interior of the air guide pipe through the exhaust hose.

[0027] According to the above content, when the bacteria are fermented, Figure 1 As shown, first, the staff will place the culture bottle containing liquid culture medium and bacterial strains on the limiting base 302, and press hard to connect the connecting circular tube 306 with the silicone one-way valve 305. At this time, the culture bottle will be initially limited by the combined action of the limiting base 302 and the connecting circular tube 306.

[0028] Subsequently, the rectangular shaking table 301 will oscillate under the action of the automatic control mechanism 5. The movement of the rectangular shaking table 301 will cause the culture bottle assembly 303 to move synchronously, thereby oscillating and culturing the bacteria uniformly to ensure that the bacteria can enter the logarithmic growth phase synchronously.

[0029] Moreover, during the shaking culture process, oxygen will first enter the gas duct through the exhaust hose, and then enter the interior of the culture bottle through the connecting tube 306 and the silicone one-way valve 305 (using a conical microporous structure, the pore size gradient is designed as: inlet 50μm→outlet 10μm), thereby achieving bottom aeration, which can effectively improve the dissolved oxygen efficiency in the liquid culture medium during high-density culture.

[0030] In this embodiment, by setting up the shaking culture mechanism 3 and utilizing the mutual cooperation between the rectangular shaking table 301 and the oxygen supply component 307, eddy currents can be generated through the horizontal circular motion of the rectangular shaking table 301 to enhance the oxygen transfer at the gas-liquid interface, and microbubbles can be directly injected from the bottom of the culture bottle to break through the oxygen diffusion limitation during high-density culture, thereby effectively improving the aeration efficiency and the synchronization of bacterial growth; moreover, in this embodiment, by setting up the shaking culture mechanism 3 and utilizing the mutual cooperation between the connecting circular tube 306 and the silicone one-way valve 305, rapid sealing installation is achieved, which can greatly shorten the operation time, avoid leakage of the gas path, and is easy to use.

[0031] Embodiment 2 In order to prevent the bottle from tipping over or the liquid from splashing during high-speed shaking and to ensure the stability during long-term shaking culture, based on Example 1, Figure 1 , Figure 2 as well as Figure 7-Figure 9 As shown, the present embodiment is provided with a splash-proof auxiliary mechanism 4. Specifically, the splash-proof auxiliary mechanism 4 includes a lifting push rod 401 fixedly mounted on the rectangular shaking table 301. A mounting bracket 402 is arranged at the upper end of the lifting push rod 401. A limiting tube cover 403 is fixedly mounted on the mounting bracket 402. The cross-sectional diameter of the limiting tube cover 403 is smaller than the cross-sectional diameter of the middle part of the culture bottle. The number of limiting tube covers 403 matches the number of limiting bases 302. During the downward movement of the limiting tube cover 403, the rubber flange 404 will contact the outer side of the culture bottle, thereby further pressing and limiting the culture bottle assembly 303. A rubber flange 404 is fixedly mounted on the inner side wall of the limiting tube cover 403. A triangular support is fixedly mounted on the upper end of the limiting tube cover 403. The support frame 405 is provided with a DO sensor 406 for detecting the dissolved oxygen content coaxially mounted at the lower end of the triangular support frame 405. The DO sensor 406 is electrically connected to the oxygen supply assembly 307 through a wire. Through the real-time monitoring data of the DO sensor 406, a closed-loop feedback system is formed with the oxygen supply assembly 307, so as to accurately adjust the oxygen flow rate of the bottom aeration. The triangular support frame 405 is provided with a hydrophobic breathable membrane 407. During the shaking culture process, the lifting push rod 401 will automatically shrink downward, so that the limiting tube cover 403 is sleeved on the top of the culture bottle. The hydrophobic breathable membrane 407 is a PTFE membrane, which allows gas exchange but prevents water evaporation. During the long-term shaking culture process, the evaporation of the culture solution can be effectively reduced, thereby avoiding the influence on the concentration of the culture medium and the growth of the bacteria.

[0032] According to the above content, after the staff puts the culture bottle assembly 303 on the limiting base 302 as required, the lifting push rod 401 will automatically shrink downward to make the mounting bracket 402 move vertically downward.

[0033] The downward movement of the mounting bracket 402 will cause the limiting tube cover 403 to be sleeved on the outside of the culture bottle. Moreover, as the mounting bracket 402 continues to move downward, the annular rubber flange 404 will contact the outside of the culture bottle, thereby performing secondary positioning of the culture bottle, which can effectively prevent the culture bottle from tipping over during long-term shaking culture.

[0034] Moreover, when the mounting bracket 402 moves downward, the probe portion of the DO sensor 406 will extend into the interior of the liquid culture medium. During the shaking culture process, the DO sensor 406 will monitor the dissolved oxygen content in the liquid culture medium in real time. Subsequently, the air intake of the oxygen supply component 307 will be automatically adjusted according to the measured dissolved oxygen content.

[0035] In addition, after the mounting bracket 402 is moved downward, the hydrophobic breathable membrane 407 will just cover the mouth of the culture bottle, which can effectively reduce the evaporation of the culture solution during long-term (eg, 24 to 72 hours) shaking culture.

[0036] In this embodiment, the splash-proof auxiliary mechanism 4 is provided, and the cooperative pressing fit between the lifting and limiting tube cover 403 and the rubber flange 404 is utilized to realize the secondary mechanical fixation of the culture bottle, which can effectively prevent the bottle body from tipping over or the liquid from splashing during high-speed oscillation, and ensure the stability during long-term oscillation culture; moreover, in this embodiment, the splash-proof auxiliary mechanism 4 is provided, and the DO sensor 406 is automatically inserted into the culture medium as the tube cover moves downward, dynamically feeds back the dissolved oxygen data and links the oxygen supply component 307 to adjust the aeration volume, which can effectively ensure the dissolved oxygen accuracy, and the hydrophobic breathable membrane 407 covers the bottle mouth, which can effectively reduce the water loss of the culture medium and avoid the interference of the concentration change of the culture medium on the growth of the bacteria.

[0037] Embodiment 3 In order to improve the environmental adaptability and process controllability of the equipment when culturing different strains, based on the above embodiments, Figure 1 , Figure 2 as well as Figure 3 As shown, the present embodiment is provided with an automatic control mechanism 5, specifically, the automatic control mechanism 5 includes a control panel 501 fixedly mounted on the equipment housing 1, a temperature control component 502 is arranged inside the fermentation cavity 2, an oscillation drive component 503 for driving the rectangular shaking table 301 to perform eccentric circular motion is arranged at the bottom of the equipment housing 1, a movable cover plate 504 is rotatably connected to the upper end of the fermentation cavity 2, a buffer convex strip 505 is fixedly mounted on the inner wall of the fermentation cavity 2, a silicone handle 506 is fixedly mounted on the outer side of the movable cover plate 504, the temperature control component 502 can automatically adjust the temperature and humidity environment inside the fermentation cavity 2 according to the program setting of the staff, and the oscillation drive component 503 is mainly composed of a stepping motor, which can be programmed to accurately control the rotation speed, direction and displacement to realize complex oscillation modes (such as linear / circular compound motion).

[0038] According to the above content, after the culture bottle is placed, the staff will first cover the movable cover 504, and then use the control panel 501 to set the parameters. After the parameters are set, the temperature control component 502 will adjust the temperature and humidity in the fermentation cavity 2, and the oscillation drive component 503 will drive the rectangular shaker 301 to oscillate, thereby completing the fermentation culture of the strain.

[0039] This embodiment provides an automatic control mechanism 5 and utilizes the coordinated cooperation of the temperature control component 502 and the oscillation drive component 503 to not only accurately control the temperature and humidity environment in the fermentation cavity 2, but also realize intelligent switching of various oscillation modes (such as circular / linear compound motion) through a programmable stepper motor, thereby significantly improving the environmental adaptability and process controllability of culturing different strains.

[0040] Embodiment 4 In order to improve the stability of the device when shaking and culturing the strains as much as possible, based on the above embodiments, Figure 1 , Figure 3 as well as Fig.10 As shown, the present embodiment is provided with an anti-skid shock absorbing mechanism 6. Specifically, the anti-skid shock absorbing mechanism 6 includes a mounting sleeve 601 fixedly mounted on the bottom of the device housing 1, an adjusting screw 603 is movably mounted inside the mounting sleeve 601, an anti-skid silicone pad 602 is fixedly mounted on the lower end of the adjusting screw 603, the anti-skid silicone pad 602 is coaxially arranged with the adjusting screw 603, and the adjusting screw 603 is threadedly connected to the mounting sleeve 601.

[0041] According to the above content, when the installation plane is uneven, the staff can turn the anti-slip silicone pad 602 to make the adjustment screw 603 rotate synchronously. When the adjustment screw 603 rotates, it will move up or down, thereby quickly leveling the equipment, which can effectively improve the stability of the equipment during the shaking culture process.

[0042] Moreover, the anti-slip silicone pad 602 can effectively increase the friction between the device and the installation surface, preventing the device from shifting during operation.

[0043] This embodiment provides an anti-skid shock-absorbing mechanism 6, and utilizes the mutual cooperation between the mounting sleeve 601 and the adjusting screw 603, which can not only realize the rapid leveling of the equipment (single adjustment time <30 seconds), but also effectively eliminate the shaking of the equipment caused by the uneven ground (the vibration amplitude is reduced by more than 60%), thereby effectively ensuring the stability during the cultivation process; moreover, this embodiment provides an anti-skid shock-absorbing mechanism 6, and utilizes the synergistic effect of the anti-skid silicone pad 602 and the adjusting screw 603, which can not only provide high friction fixation (static friction coefficient ≥ 0.8), but also absorb the vibration energy of the equipment during operation (shock absorption efficiency reaches 45%), thereby significantly extending the service life of the equipment.

[0044] The control method of the present invention is automatic control through a controller. The control circuit of the controller can be implemented by simple programming by technicians in this field. The provision of power is also common knowledge in this field. The present invention is mainly used to protect mechanical devices, so the present invention will no longer explain the control method and circuit connection in detail.

[0045] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0046] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A fermentation device based on the preparation of a microbial inoculant, comprising a device housing (1), wherein a fermentation cavity (2) is provided inside the device housing (1), and characterized in that: The fermentation cavity (2) is provided with an oscillating culture mechanism (3) and an anti-splashing auxiliary mechanism (4) for supplying oxygen to the culture liquid and thus improving the dissolved oxygen efficiency; an automatic control mechanism (5) for adjusting the bacterial growth environment is provided on the device housing (1); an anti-skid shock absorbing mechanism (6) for leveling the device is provided at the lower end of the device housing (1); the anti-splashing auxiliary mechanism (4) can adjust the aeration rate of the oscillating culture mechanism (3) by detecting the dissolved oxygen content in the culture bottle; and the anti-skid shock absorbing mechanism (6) and the oscillating culture mechanism (3) can cooperate to achieve bidirectional shock absorption.

2. The fermentation equipment based on microbial inoculant preparation according to claim 1, characterized in that: The shaking culture mechanism (3) comprises a rectangular shaking table (301) movably mounted inside the fermentation cavity (2), an air guide duct being buried inside the rectangular shaking table (301), a plurality of limiting bases (302) being fixedly mounted on the rectangular shaking table (301), a culture bottle assembly (303) being detachably mounted on the limiting base (302), the bottom of the culture bottle assembly (303) being recessed inwardly to form a circular groove (304), a silicone one-way valve (305) being arranged inside the circular groove (304), a connecting circular tube (306) being coaxially fixedly mounted on the limiting base (302), and the connecting circular tube (306) being connected to the silicone one-way valve (305).

3. The fermentation equipment based on microbial inoculant preparation according to claim 2, characterized in that: The device housing (1) is provided with an oxygen supply assembly (307), the oxygen supply assembly (307) comprising an oxygen valve fixedly mounted on the outside of the device housing (1), the air inlet end of the oxygen valve being connected to the gas storage tank, the air outlet end of the oxygen valve being connected to an exhaust hose, and the exhaust hose being connected to the air guide pipe.

4. The fermentation equipment based on microbial inoculant preparation according to claim 2, characterized in that: An exhaust passage for connecting the air guide pipe and the connecting circular tube (306) is coaxially provided on the limiting base (302).

5. The fermentation equipment based on microbial inoculant preparation according to claim 2, characterized in that: The splash-proof auxiliary mechanism (4) comprises a lifting push rod (401) fixedly mounted on the rectangular shaking table (301); a mounting bracket (402) is arranged at the upper end of the lifting push rod (401); a limit tube cover (403) is fixedly mounted on the mounting bracket (402); a rubber flange (404) is fixedly mounted on the inner side wall of the limit tube cover (403); a triangular bracket (405) is fixedly mounted on the upper end of the limit tube cover (403); a DO sensor (406) for detecting dissolved oxygen is coaxially mounted on the lower end of the triangular bracket (405); and a hydrophobic breathable membrane (407) is arranged on the triangular bracket (405).

6. The fermentation equipment based on microbial inoculant preparation according to claim 5, characterized in that: The cross-sectional diameter of the position-limiting tube cover (403) is smaller than the cross-sectional diameter of the culture bottle assembly (303) at S, and the number of the position-limiting tube covers (403) matches the number of the position-limiting bases (302).

7. The fermentation equipment based on microbial inoculant preparation according to claim 5, characterized in that: The hydrophobic breathable membrane (407) is a PTFE membrane, which allows gas exchange but prevents water evaporation.

8. The fermentation equipment based on microbial inoculant preparation according to claim 5, characterized in that: The DO sensor (406) is electrically connected to the oxygen supply component (307) via a wire.

9. The fermentation equipment based on microbial inoculant preparation according to claim 2, characterized in that: The automatic control mechanism (5) comprises a control panel (501) fixedly mounted on the device housing (1); a temperature control component (502) is arranged inside the fermentation cavity (2); an oscillation drive component (503) for driving the rectangular shaking table (301) to perform eccentric circular motion is arranged at the bottom of the device housing (1); a movable cover plate (504) is rotatably connected to the upper end of the fermentation cavity (2); a buffer convex strip (505) is fixedly mounted on the inner wall of the fermentation cavity (2); and a silicone handle (506) is fixedly mounted on the outer side of the movable cover plate (504).

10. The fermentation equipment based on microbial inoculant preparation according to claim 1, characterized in that: The anti-skid shock-absorbing mechanism (6) comprises a mounting sleeve (601) fixedly mounted on the bottom of the device housing (1), an adjusting screw (603) being movably mounted inside the mounting sleeve (601), and an anti-skid silicone pad (602) being fixedly mounted at the lower end of the adjusting screw (603).

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