Culture device for stem cells

Abstract

The application provides a culture device for stem cells, which comprises a culture box body, a top shell fixedly installed on the top of the culture box body, a controller fixedly installed on one side of the culture box body and a rotating arm movably installed in the culture box body, and a heating installation plate is fixedly installed on the bottom of the inner wall of the culture box body. The application can quickly transfer stem cells to temperature positions suitable for different stages, can simultaneously culture stem cells in different stages, can synchronously culture stem cells in different stages and ensure that the transferred stem cells have suitable transfer temperature, so as to solve the problems that in the existing temperature box culture process, stem cells in the container cannot quickly obtain the required culture temperature, the stem cells are not in the same culture stage, the required temperature is different, the stem cells in different stages cannot be directly placed in the required temperature culture position after being added, and the culture stem cells do not have a taking and temporary storage transfer position.

Description

Technical Field

[0001] This invention relates to the field of cell culture technology, and more particularly to a stem cell culture device. Background Technology

[0002] Stem cells are a type of cell with self-renewal and multi-lineage differentiation potential, demonstrating enormous application potential in numerous fields such as medical research, disease treatment, and regenerative medicine. As stem cell research continues to deepen, the demand for efficient and precise culture devices to support it is becoming increasingly prominent.

[0003] In recent years, stem cell research has achieved numerous breakthroughs. For example, in the field of regenerative medicine, stem cells are expected to be used to repair damaged tissues and organs, such as using mesenchymal stem cells to treat osteoarthritis, myocardial infarction, and other diseases. In drug development, stem cells can serve as in vitro models to screen and evaluate the efficacy and safety of drugs, and compared to traditional animal models, they can more accurately reflect the effects of drugs on human cells. This expansion of applications has led to a dramatic increase in demand for large quantities of high-quality, functionally stable stem cells.

[0004] Existing stem cell culture devices have the following technical problems:

[0005] If the temperature is not suitable when culturing in a uniform temperature chamber, the stem cells in the vessel cannot quickly reach the required culture temperature.

[0006] When multiple stem cells are cultured simultaneously, if they are not at the same culture stage, the required temperature will be different, and it cannot be guaranteed that newly added stem cells at different stages can be directly placed in the culture position at the required temperature.

[0007] It lacks a temporary storage location for cell culture after removal. When removing the cells, cooling will affect the cell culture viability, leading to an imbalance of internal and external ion concentrations and reducing the survival rate. Summary of the Invention

[0008] To address the shortcomings of existing technologies, the present invention aims to provide a stem cell culture device that can rapidly transfer stem cells to temperature positions suitable for different stages, simultaneously culture stem cells at different stages, and ensure that stem cells at different stages are cultured at the same time while ensuring that they are transferred at the appropriate temperature. This solves the problems of existing temperature chambers where the temperature is not suitable during culture, stem cells in the vessel cannot quickly reach the required culture temperature, different temperatures are required for different culture stages, and newly added stem cells at different stages cannot be directly placed in the required temperature culture position. It also lacks a temporary transfer position for removing and storing cells after culture.

[0009] To achieve the above objectives, the present invention is implemented through the following technical solution: a stem cell culture device, comprising a culture chamber, a top shell fixedly installed on the top of the culture chamber, a controller fixedly installed on one side of the culture chamber, and a rotating arm movably installed inside the culture chamber. A heating mounting plate is fixedly installed on the bottom of the inner wall of the culture chamber. A culture partition is fixedly installed inside the culture chamber, located at the bottom of the rotating arm and at the top of the heating mounting plate. Eight sets of annularly distributed culture dish placement holes are opened around the top of the culture partition. Each of the eight sets of culture dish placement holes is provided with a sealing cap that seals with it. A transfer hole is opened at the center of the top of the culture partition. A cell culture dish is placed inside the culture dish placement hole. An electric heating plate is fixedly installed inside the heating mounting plate, located at the bottom of the culture dish placement hole and the transfer hole. The device also includes a culture dish conversion mechanism fixedly installed on the top of the inner wall of the top shell. The culture dish conversion mechanism is used to drive the rotating arm to rotate inside the culture chamber to determine the position of the culture dish to be converted.

[0010] The vessel-holding extraction mechanism is slidably installed inside the rotating arm and is used to hold the culture vessel together for extraction and placement operations;

[0011] A transverse drive mechanism is slidably mounted inside the rotating arm and is used to drive the dish to press against the extraction mechanism for displacement;

[0012] The rotating synchronous flip-top drive mechanism is fixedly installed on the bottom of the rotating arm near the inner wall of the incubator, and is used to drive the sealing cover to flip open while the rotating arm rotates.

[0013] Furthermore, the culture dish conversion mechanism includes a top frame, a reinforcing plate, a stabilizing frame, and a servo motor. The top frame is provided in four sets and is arranged in a ring on the top of the inner wall of the top shell and is fixedly installed with the top shell. The reinforcing plate is fixedly installed at the bottom of the four sets of top frames. The stabilizing frame is rotatably installed at the bottom of the reinforcing plate. The servo motor is fixedly installed at the center of the top of the inner wall of the top shell. The output end of the servo motor passes through the reinforcing plate and extends to the top of the stabilizing frame and is fixedly installed with the stabilizing frame.

[0014] Furthermore, the vessel clamping and extraction mechanism includes an electric push rod one, a lifting rod, a fixing frame, an electric push rod two, a clamping plate, and an expansion assembly. The electric push rod one is slidably installed inside the rotating arm. The lifting rod is fixedly installed at the output end of the electric push rod one. The fixing frame is sleeved and fixedly installed on the top of the surface of the lifting rod. The electric push rod two is fixedly installed on the side of the fixing frame away from the lifting rod. The clamping plate is slidably sleeved on the bottom of the surface of the lifting rod. The top of the clamping plate is fixedly installed with the output end of the electric push rod two. The expansion assembly is located at the bottom of the lifting rod and is used to tension the cell culture dish.

[0015] Furthermore, the expansion assembly includes a clamping ring, a connecting ring, a pushing ring frame, a scissor-type telescopic frame, a fixed seat, a tensioning plate, and a second spring. The clamping ring is slidably mounted on the surface of the lifting rod and fixedly mounted to the bottom of the clamping plate. The connecting ring is detachably mounted on the bottom of the clamping ring by bolts. The pushing ring frame is rotatably sleeved on the top of the outer surface of the connecting ring. Three sets of scissor-type telescopic frames are provided, and the top of the three sets of scissor-type telescopic frames on the side near the pushing ring frame is hinged to the pushing ring frame. The top of the scissor-type telescopic frame on the side away from the pushing ring frame is slidably mounted to the tensioning plate. The bottom of the scissor-type telescopic frame on the side away from the pushing ring frame is fixedly hinged to the bottom of the tensioning plate on the side near the lifting rod. The bottom of the scissor-type telescopic frame on the side near the lifting rod is hinged to the fixed seat. The second spring is sleeved on the surface of the lifting rod, the top of the second spring contacts the bottom of the connecting ring, and the bottom of the second spring is fixedly mounted to the top of the fixed seat.

[0016] Furthermore, the transverse drive mechanism includes a linear motor stator, a linear motor mover, and a drive frame. The linear motor stator is fixedly mounted on the back of the rotating arm, the linear motor mover is mounted on the surface of the linear motor stator, the drive frame is fixedly mounted on the top of the linear motor mover, and the drive frame is fixedly mounted to the bottom of the electric push rod. The output end of the electric push rod passes through the drive frame and is in transmission cooperation with the lifting rod.

[0017] Furthermore, the synchronous flip-top drive mechanism includes an annular lifting guide frame, a limiting cylinder, a sliding rod, a roller, an arc-shaped toothed plate, a spring, and a power conversion flip-top assembly. The annular lifting guide frame is fixedly installed on the top of the culture partition. The limiting cylinder is fixedly installed on the bottom of the rotating arm near the inner wall of the culture chamber. The sliding rod is slidably installed inside the limiting cylinder. The roller is rotatably installed on the bottom of the sliding rod via a pin. The arc-shaped toothed plate is fixedly installed on the bottom of the sliding rod near the culture partition. The spring is located inside the limiting cylinder. The top of the spring is fixedly installed to the top of the inner wall of the limiting cylinder, and the bottom of the spring is fixedly installed to the top of the sliding rod. The power conversion flip-top assembly is located on the culture partition and has eight annularly distributed on the side of the culture dish placement hole away from the central transfer hole. The power conversion flip-top assembly is used to convert the torsional force of the rotating arm to open the sealing cover for easy placement and removal of cell culture dishes.

[0018] Furthermore, the power conversion flip-top assembly includes a support frame, a vertical rod, a first drive gear, a first bevel gear, a second bevel gear, a transverse drive rod, a second drive gear, a third drive gear, and a bracket. The support frame is fixedly installed on the top of the culture partition, away from the transfer hole and close to the sealing cover. The vertical rod is longitudinally rotatably installed on one side of the top of the support frame. The first drive gear is fixedly installed on the top of the vertical rod and meshes with the arc-shaped toothed plate. The first bevel gear is fixedly installed on the bottom of the surface of the vertical rod. The transverse drive rod is laterally rotatably installed inside the support frame. The second bevel gear is fixedly installed on the side of the transverse drive rod close to the first bevel gear and meshes with the first bevel gear. The second drive gear is fixedly installed on the side of the transverse drive rod away from the second bevel gear. The bracket is fixedly installed on the top of the culture partition and located on both sides between the support frame and the sealing cover. The third drive gear is fixedly installed on the side of the sealing cover close to the second drive gear and meshes with the second drive gear. The side of the sealing cover close to the support frame is rotatably engaged with the bracket via a pin.

[0019] Furthermore, a maintenance plate is detachably installed on one bottom side of the incubator, and the four corners of the maintenance plate are magnetically fixed to the incubator by magnetic blocks.

[0020] Furthermore, a temperature sensor is fixedly installed at the bottom of the sealing cap, and limiting protrusions are fixedly installed inside both the culture dish placement hole and the transfer hole, thus providing longitudinal limiting fit for the cell culture dish.

[0021] The beneficial effects of this invention are as follows: This invention uses a transfer hole to transfer vessels that require rapid temperature changes during culture. The vessel transfer mechanism drives the rotating arm to rotate, which, in conjunction with a lateral drive mechanism, pushes the vessel against the extraction mechanism, allowing the cell culture vessel to be extracted and moved to the placement hole at the required temperature. A temperature-adaptive electric heating plate is then used to culture the cell culture vessel at the corresponding stage. Simultaneously, as the rotating arm flips, a synchronous flip-top drive mechanism opens the sealing cap at the desired transfer position, facilitating the extraction and transfer operation and improving the efficiency and temperature adaptability of cell culture transfer. Attached Figure Description

[0022] Other features, objects, and advantages of the present invention will become more apparent from the following detailed description of non-limiting embodiments with reference to the accompanying drawings:

[0023] Figure 1 This is a schematic diagram of the structure of the present invention;

[0024] Figure 2 This is a schematic diagram of the half-section three-dimensional structure of the present invention;

[0025] Figure 3 This is a schematic diagram of the exploded structure of the culture partition of the present invention;

[0026] Figure 4 This is a three-dimensional structural diagram of the sliding rod of the present invention;

[0027] Figure 5 For the present invention Figure 4 Another perspective structural diagram;

[0028] Figure 6 This is an exploded three-dimensional structural diagram of the rotating arm of the present invention;

[0029] Figure 7 This is an exploded three-dimensional structural diagram of the tensioning plate of the present invention.

[0030] In the diagram: 1. Incubator body; 11. Top shell; 12. Controller; 13. Inspection plate; 2. Rotating arm; 201. Top frame; 202. Reinforcing plate; 203. Stabilizing frame; 204. Servo motor; 21. Limiting cylinder; 211. Sliding rod; 212. Roller; 213. Arc-shaped toothed plate; 214. Spring 1; 22. Linear motor stator; 221. Linear motor mover; 222. Drive frame; 223. Electric push rod 1; 224. Lifting rod; 2241. Fixed frame; 2242. Electric push rod 2; 2243. Clamping plate; 2244. Clamping ring; 22441. Connecting ring; 22442. Pushing ring 1. Frame; 22443. Scissor-type telescopic frame; 22444. Fixed base; 22445. Tensioning plate; 22446. Spring II; 3. Culture partition; 31. Annular lifting guide frame; 32. Transfer hole; 33. Limiting protrusion ring; 34. Culture dish placement hole; 35. Cell culture dish; 301. Sealing cap; 3011. Temperature sensor; 341. Support frame; 342. Upright pole; 343. Drive gear I; 344. Bracket; 345. Bevel gear I; 346. Bevel gear II; 347. Horizontal drive rod; 348. Drive gear II; 349. Drive gear III; 4. Heating mounting plate; 41. Electric heating plate. Detailed Implementation

[0031] To make the technical means, creative features, objectives and effects of this invention easier to understand, the invention will be further described below in conjunction with specific embodiments.

[0032] Please see Figure 1 and Figure 2 , Figure 1 This is a schematic diagram of the structure of the present invention; Figure 2 This is a schematic diagram of the half-section three-dimensional structure of the present invention.

[0033] A stem cell culture device includes a culture chamber 1, a top shell 11 fixedly installed on the top of the culture chamber 1, a controller 12 fixedly installed on one side of the culture chamber 1, and a rotating arm 2 movably installed inside the culture chamber 1. A heating mounting plate 4 is fixedly installed on the bottom of the inner wall of the culture chamber 1. A culture partition 3 is fixedly installed inside the culture chamber 1, located at the bottom of the rotating arm 2 and above the heating mounting plate 4. Eight sets of annularly distributed culture dish placement holes 34 are formed around the top of the culture partition 3. Each of the eight sets of culture dish placement holes 34 is provided with a sealing device for sealing. The cover 301 has a transfer hole 32 at the top center of the culture partition 3. The culture dish placement hole 34 is used to house a cell culture dish 35. The heating mounting plate 4 is fixedly installed inside the heating mounting plate 4. The electric heating plate 41 is located at the bottom of the culture dish placement hole 34 and the transfer hole 32. The electric heating plate 41 is electrically connected to the controller 12. The controller 12 is used to control the temperature of the electric heating plate 41. The cover 301 also includes a culture dish conversion mechanism fixedly installed on the top of the inner wall of the top shell 11. The culture dish conversion mechanism is used to drive the rotating arm 2 to rotate inside the culture chamber 1 to determine the position of the culture dish to be converted.

[0034] Please see Figures 2 to 7 As shown, Figure 2 This is a schematic diagram of the half-section three-dimensional structure of the present invention; Figure 3 This is a schematic diagram of the exploded structure of the culture partition of the present invention; Figure 4 This is a three-dimensional structural diagram of the sliding rod of the present invention; Figure 5 For the present invention Figure 4 Another perspective structural diagram; Figure 6 This is an exploded three-dimensional structural diagram of the rotating arm of the present invention; Figure 7 This is an exploded three-dimensional structural diagram of the tensioning plate of the present invention.

[0035] The petri dish conversion mechanism includes a top frame 201, a reinforcing plate 202, a stabilizing frame 203, and a servo motor 204. Four sets of top frames 201 are arranged in a ring around the top of the inner wall of the top shell 11 and are fixedly installed thereto. The reinforcing plate 202 is fixedly installed at the bottom of the four sets of top frames 201. The stabilizing frame 203 is rotatably installed at the bottom of the reinforcing plate 202. The servo motor 204 is fixedly installed at the center of the top of the inner wall of the top shell 11. The output end of the servo motor 204 passes through the reinforcing plate 202 and extends to the top of the stabilizing frame 203, where it is fixedly installed. The top frame 201 facilitates the fixing of the reinforcing plate 202 to the top shell 11. The stabilizing frame 203, which rotatably engages with the reinforcing plate 202, provides the rotating arm 2 with anti-bending force during rotation. Starting the servo motor 204 drives the stabilizing frame 203 to rotate through its output end through the reinforcing plate 202. Subsequently, the stabilizing frame 203 drives the rotating arm 2 to rotate synchronously, moving it to the desired conversion position.

[0036] The vessel clamping and extraction mechanism is slidably installed inside the rotating arm 2 and is used to clamp the culture vessel for extraction and placement operations. The vessel clamping and extraction mechanism includes an electric push rod 223, a lifting rod 224, a fixing frame 2241, an electric push rod 2242, a clamping plate 2243, and an expansion assembly. The electric push rod 223 is slidably installed inside the rotating arm 2. The lifting rod 224 is fixedly installed at the output end of the electric push rod 223. The fixing frame 2241 is sleeved and fixed to the top of the surface of the lifting rod 224. The electric push rod 2242 is fixedly installed on the side of the fixing frame 2241 away from the lifting rod 224. The clamping plate 2243 is slidably sleeved on the bottom of the surface of the lifting rod 224. The top of the clamping plate 2243 is fixedly installed with the output end of the electric push rod 2242. The expansion assembly is located at the bottom of the lifting rod 224 and is used to tension the cell culture dish 35. When the electric push rod 223 is activated, it can drive the lifting rod 224 to move up and down through the output end. When the lifting rod 224 moves up and down, it can drive the fixed frame 2241 and the expansion component at the bottom to move up and down synchronously. At the same time, when the electric push rod 2242 needs to be activated, it can drive the clamping plate 2243 to move downward. The clamping plate 2243 drives the expansion component to expand and tighten the cell culture dish 35 to facilitate extraction and placement operations.

[0037] The expansion assembly includes a clamping ring 2244, a connecting ring 22441, a pushing ring frame 22442, a scissor-type telescopic frame 22443, a fixing base 22444, a tensioning plate 22445, and a spring 22446. The clamping ring 2244 is slidably mounted on the surface of the lifting rod 224 and fixedly mounted to the bottom of the clamping plate 2243. The connecting ring 22441 is detachably mounted to the bottom of the clamping ring 2244 by bolts. The pushing ring frame 22442 is rotatably sleeved on the top of the outer surface of the connecting ring 22441. Three sets of scissor-type telescopic frames 22443 are provided, and the top of the three sets of scissor-type telescopic frames 22443 on the side closest to the pushing ring frame 22442 is connected to... The push ring frame 22442 is hinged. The top of the scissor-type telescopic frame 22443 on the side away from the push ring frame 22442 is slidably installed with the tension plate 22445. The bottom of the scissor-type telescopic frame 22443 on the side away from the push ring frame 22442 is fixedly hinged with the bottom of the tension plate 22445 on the side near the lifting rod 224. The bottom of the scissor-type telescopic frame 22443 on the side near the lifting rod 224 is hinged with the fixed seat 22444. The second spring 22446 is sleeved on the surface of the lifting rod 224. The top of the second spring 22446 contacts the bottom of the connecting ring 22441. The bottom of the second spring 22446 is fixedly installed with the top of the fixed seat 22444.

[0038] When the clamping plate 2243 is driven downward by the electric push rod 2242, it can drive the clamping ring 2244 to move downward synchronously. The clamping ring 2244 then compresses and pushes the ring frame 22442 downward. Subsequently, the ring frame 22442 is pushed through the hinged scissor-type telescopic frame 22443 so that the top and bottom of the side near the lifting rod 224 are brought closer together. This pushes the tension plate 22445 outward to expand and clamp the cell culture dish 35, facilitating extraction of the cell culture dish 35 after clamping. Simultaneously, the spring 22446... The elastic force is compressed and contracted by the downward squeezing force of the connecting ring 22441. When contraction is required, the output end of the electric push rod 2242 can be activated again to drive the clamping plate 2243 to move upward. Then, the elastic force of the spring 22446 is released, which can drive the push ring frame 22442, the connecting ring 22441 and the clamping ring 2244 to move upward synchronously, forcing the two ends of the top of the scissor-type telescopic frame 22443 to move closer to each other, thereby driving the tension plate 22445 to retract. At this time, the cell culture dish 35 can lose the clamping force of the tension plate 22445, thus making it easier to place.

[0039] A lateral drive mechanism, slidably mounted inside the rotating arm 2, is used to drive the cell culture dish to move against the extraction mechanism. The lateral drive mechanism includes a linear motor stator 22, a linear motor mover 221, and a drive frame 222. The linear motor stator 22 is fixedly mounted on the back of the rotating arm 2, the linear motor mover 221 is mounted on the surface of the linear motor stator 22, and the drive frame 222 is fixedly mounted on the top of the linear motor mover 221. The drive frame 222 is fixedly mounted to the bottom of an electric push rod 223, and the output end of the electric push rod 223 passes through the drive frame 222 and is driven by a lifting rod 224. After the linear motor stator 22 and the linear motor mover 221 are driven together, the linear motor mover 221 can slide laterally on the linear motor stator 22, and the drive frame 222 can then drive the electric push rod 223 laterally, moving the cell culture dish 35 fixed on the tension plate 22445 to the desired transfer position.

[0040] A synchronous flip-top drive mechanism is fixedly installed at the bottom of the rotating arm 2 near the inner wall of the culture chamber 1, and is used to drive the sealing cover 301 to flip open while rotating with the rotating arm 2. The synchronous flip-top drive mechanism includes an annular lifting guide frame 31, a limiting cylinder 21, a sliding rod 211, a roller 212, an arc-shaped toothed plate 213, a spring 214, and a power conversion flip-top assembly. The annular lifting guide frame 31 is fixedly installed on the top of the culture partition 3, the limiting cylinder 21 is fixedly installed at the bottom of the rotating arm 2 near the inner wall of the culture chamber 1, the sliding rod 211 is slidably installed inside the limiting cylinder 21, and the roller 212... A pivot pin is rotatably mounted on the bottom of the sliding rod 211. An arc-shaped toothed plate 213 is fixedly mounted on the bottom of the sliding rod 211 near the culture partition 3. A spring 214 is located inside the limiting cylinder 21. The top of the spring 214 is fixedly mounted to the top of the inner wall of the limiting cylinder 21, and the bottom of the spring 214 is fixedly mounted to the top of the sliding rod 211. A power conversion flip-top assembly is mounted on the culture partition 3 and has eight sets of rings distributed on the side of the culture dish placement hole 34 away from the transfer hole 32. The power conversion flip-top assembly is used to convert the torsional force of the rotating arm 2 to open the sealing cover 301 for easy handling of cell culture dishes 35.

[0041] The annular lifting guide frame 31 provides guidance for the movement trajectory of the roller 212. When the rotating arm 2 rotates, the sliding rod 211 rotates accordingly. Subsequently, the arc-shaped toothed plate 213 maintains a downward elastic force on the sliding rod 211, ensuring that the roller 212 at the bottom of the sliding rod 211 contacts the top of the annular lifting guide frame 31. This facilitates the roller 212 to change its height according to the slope of the annular lifting guide frame 31. When the roller 212 is at the top position on the annular lifting guide frame 31, the arc-shaped toothed plate 213 and the drive gear 343 will not mesh. When the roller 212 is at the bottom position on the annular lifting guide frame 31, the arc-shaped toothed plate 213 and the drive gear 343 will mesh, thereby driving the power conversion flip-top assembly.

[0042] The power conversion flip-top assembly includes a support frame 341, a vertical rod 342, a first drive gear 343, a first bevel gear 345, a second bevel gear 346, a transverse drive rod 347, a second drive gear 348, a third drive gear 349, and a bracket 344. The support frame 341 is fixedly installed on the top of the culture partition 3, away from the central hole 32 and close to the sealing cover 301. The vertical rod 342 is longitudinally rotatably installed on one side of the top of the support frame 341. The first drive gear 343 is fixedly installed on the top of the vertical rod 342 and meshes with the arc-shaped toothed plate 213. The first bevel gear 345 is fixedly installed on the bottom of the surface of the vertical rod 342. The transverse drive rod 347 is transversely rotatably installed on the support frame 344. Inside the support frame 341, bevel gear 2 346 is fixedly installed on the side of the transverse drive rod 347 near bevel gear 1 345 and meshes with bevel gear 1 345. Drive gear 2 348 is fixedly installed on the side of the transverse drive rod 347 away from bevel gear 2 346. Support 344 is fixedly installed on the top of the culture partition 3 and located on both sides between the support frame 341 and the sealing cover 301. Drive gear 3 349 is fixedly installed on the side of the sealing cover 301 near drive gear 2 348 and meshes with drive gear 2 348. The side of the sealing cover 301 near the support frame 341 is rotatably engaged with support 344 through a shaft pin.

[0043] When the rotating arm 2 rotates and drives the roller 212 to the bottom position on the annular lifting guide frame 31, the arc-shaped toothed plate 213 can drive the drive gear 343 to rotate through meshing with the drive gear 343. After the drive gear 343 rotates, it can drive the bevel gear 345 to rotate through the upright rod 342. Then, the bevel gear 345 drives the bevel gear 346 to rotate. The rotation of the bevel gear 346 can drive the transverse drive rod 347 to rotate. Then, the transverse drive rod 347 drives the drive gear 348 to rotate. After the drive gear 348 meshes with the drive gear 349, it drives the drive gear 349 to rotate and drive the sealing cover 301 to rotate. This causes the sealing cover 301 to flip upward around the axis of the bracket 344, which facilitates the linear motor stator 22 on the rotating arm 2 to drive the linear motor mover 221 to drive the electric push rod 223 on the drive frame 222 to move laterally and realize the position transfer of the cell culture dish 35.

[0044] A maintenance plate 13 is detachably installed on one side of the bottom of the culture chamber 1. The four corners of the maintenance plate 13 are magnetically fixed to the culture chamber 1 by magnetic blocks. The maintenance plate 13 allows users to easily open and access the cell culture dish 35, and facilitates maintenance. The magnetic fixation also improves the ease of fixation during access.

[0045] A temperature sensor 3011 is fixedly installed at the bottom of the sealing cap 301. Limiting rings 33 are fixedly installed inside both the culture dish placement hole 34 and the transfer hole 32, providing longitudinal limiting fit for the cell culture dish 35. The temperature sensor 3011 can detect the incubation temperature of each group of culture dish placement holes 34, determining the specific temperature for each group. Simultaneously, the limiting rings 33 prevent the cell culture dish 35 from falling off after placement, improving the ease of use during culture.

[0046] Working principle: When performing multi-stage stem cell culture, the most suitable temperature position can be selected according to the current stage. The temperature sensor 3011 on the sealing cover 301 of each culture dish placement hole 34 detects the corresponding temperature to determine the most suitable position for that stage. Then, the electric heating plate 41 at the transfer hole 32 is adjusted to the appropriate transfer temperature. Once the transfer temperature is suitable, the servo motor 204 is started to drive the stabilizing frame 203 to rotate through the output end. Then, the stabilizing frame 203 drives the rotating arm 2 to rotate synchronously, so that it rotates to the transition position suitable for the current stem cell stage temperature.

[0047] As the rotating arm 2 rotates, it drives the limiting cylinder 21 and its internal sliding rod 211 to pass through the high and low guide positions of the annular lifting guide frame 31. When it moves a certain distance before reaching the appropriate position, the sliding rod 211 is driven by the elastic force of the spring 214, causing the roller 212 to be at the low position on the annular lifting guide frame 31. Then, the arc-shaped toothed plate 213 on the sliding rod 211 meshes with the drive gear 343. At this time, the rotating arm 2 continues to rotate to align with the required position. The arc-shaped toothed plate 213 drives the drive gear 343 to rotate through the meshing with the drive gear 343. After the drive gear 343 rotates, it can pass through the upright 34. 2 drives bevel gear 345 to rotate, which in turn drives bevel gear 346 to rotate. The rotation of bevel gear 346 drives the transverse drive rod 347 to rotate, which in turn drives drive gear 348 to rotate. The meshing of drive gear 348 and drive gear 349 drives drive gear 349 to rotate, which in turn drives the sealing cover 301 to rotate. This causes the sealing cover 301 to flip upward around the axis of the bracket 344, which in turn facilitates the linear motor stator 22 on the rotating arm 2 to drive the linear motor mover 221 to drive the electric push rod 223 on the drive frame 222 laterally to the top of the central hole 32.

[0048] Then, when the electric push rod 223 is activated, it can drive the lifting rod 224 to retract downward through the output end. When the lifting rod 224 extends and retracts downward, it can drive the fixed frame 2241 and the bottom expansion component to move up and down synchronously. At the same time, when the cell culture dish 35 needs to be tightened, the electric push rod 2242 can be activated to drive the clamping plate 2243 to move downward. When the clamping plate 2243 is driven downward by the electric push rod 2242, it can drive the clamping ring 2244 to move downward synchronously. Then, the clamping ring 2244 squeezes and pushes the ring frame 22442 to move downward.

[0049] Then, the ring frame 22442 is pushed so that the top and bottom of the side near the lifting rod 224 are brought closer together through the hinged scissor-type telescopic frame 22443. This pushes the tension plate 22445 outward to expand and tighten the cell culture dish 35, making it easier to extract the cell culture dish 35 after it is tightened. At the same time, the spring 22446 is compressed by the downward squeezing force of the connecting ring 22441. After the cell culture dish 35 is tightened, the electric push rod 223 is activated to drive the lifting rod 224 to move upward. This can simultaneously drive the cell culture dish 35 on the tension plate 22445 to move upward. Then, the linear motor mover 221 on the linear motor stator 22 moves the drive frame 222 and the electric push rod 223 to the position above the culture dish placement hole 34 where the required temperature is needed. Then, the electric push rod 223 is activated to move downward, which drives the cell culture dish 35 downward to the inside of the culture dish placement hole 34 through the linear motor stator 22.

[0050] At this time, the output end of the second electric push rod 2242 is activated again to drive the clamping plate 2243 to move upward. Then, the spring 22446 releases its elasticity, which drives the push ring frame 22442, the connecting ring 22441 and the clamping ring 2244 to move upward synchronously, forcing the two ends of the top of the scissor-type telescopic frame 22443 to move closer to each other, thereby driving the tension plate 22445 to retract. At this time, the cell culture dish 35 can lose the clamping of the tension plate 22445, making it easier to place. Then, the first electric push rod 223 is activated again to drive the tension plate 22445 to retract upward. Then, the servo motor 204 is activated to drive the stabilizing frame 203 and the rotating arm 2 to rotate to the high position of the annular lifting guide frame 31, so that its arc-shaped toothed plate 213 separates from the drive gear 1 343, making it easy for the sealing cover 301 to fall down again under its own weight to seal, or to the torsion spring fixed at the shaft pin connection between the bracket 344 and the sealing cover 301 to perform torsion flip sealing.

[0051] Furthermore, the above steps also allow for mutual switching between multiple sets of cells placed in the culture dish wells 34. Even when the temperature position needs to be changed in a cell culture dish 35 that is not externally added, the above steps can still be used for the switching. The simplified process is as follows:

[0052] Step 1: Temperature detection and rotating arm positioning;

[0053] The temperature of each culture dish placement hole 34 is detected by the temperature sensor 3011 at the bottom of the sealing cover 301 to determine the appropriate temperature position for the current stem cell stage.

[0054] Start the servo motor 204 to drive the stabilizer 203 and the rotating arm 2 to rotate to the corresponding position;

[0055] Step Two: Open the sealing cap and prepare for transfer;

[0056] During the rotation of the rotating arm 2, the arc-shaped toothed plate 213 on the sliding rod 211 meshes with the drive gear 343 through the synchronous flip-cover drive mechanism, driving the sealing cover 301 to flip and open upward.

[0057] The linear motor stator 22 and the linear motor mover 221 cooperate to drive the electric push rod 223 to move laterally so that the cell culture dish 35 can be transferred laterally.

[0058] Step 3: Extraction and transfer of cells into culture dishes;

[0059] The electric push rod 223 is activated to drive the lifting rod 224 to retract downwards. At the same time, the electric push rod 2242 drives the clamping plate 2243 to move downwards, and the cell culture dish 35 is tightened and extracted through the expansion component.

[0060] The linear motor stator 22 drives the linear motor mover 221 to move the electric push rod 223 laterally to above the target culture dish placement hole 34;

[0061] Restart the electric push rod 223 to move it downwards and place the cell culture dish 35 into the target culture dish placement hole 34;

[0062] Step 4: Reset the sealing cap and reset the device;

[0063] After placement is completed, the relevant components are reversed, such as restarting the electric push rod 2242 to retract the expansion component, so that the cell culture dish 35 is no longer pressed and can be placed.

[0064] The servo motor 204 is started to drive the rotating arm 2 to rotate to the high position of the annular lifting guide frame 31, so that the arc-shaped toothed plate 213 is separated from the drive gear 343, and the sealing cover 301 is reset and sealed.

[0065] The foregoing has shown and described the basic principles, main features, and advantages of the present invention. It will be apparent to those skilled in the art that the invention is not limited to the details of the exemplary embodiments described above, and that the invention can be implemented in other specific forms without departing from its spirit or essential characteristics. Therefore, the embodiments should be considered illustrative and non-limiting in all respects, and the scope of the invention is defined by the appended claims rather than the foregoing description. Thus, all variations falling within the meaning and scope of equivalents of the claims are intended to be included within the scope of the invention. No reference numerals in the claims should be construed as limiting the scope of the claims.

[0066] Furthermore, it should be understood that although this specification describes embodiments, not every embodiment contains only one independent technical solution. This narrative style is merely for clarity. Those skilled in the art should consider the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

Claims

1. A stem cell culture device, comprising a culture chamber (1), a top shell (11) fixedly installed on the top of the culture chamber (1), a controller (12) fixedly installed on one side of the culture chamber (1), and a rotating arm (2) movably installed inside the culture chamber (1), wherein a heating mounting plate (4) is fixedly installed on the bottom of the inner wall of the culture chamber (1), and a culture partition (3) is fixedly installed inside the culture chamber (1) at the bottom of the rotating arm (2) and at the top of the heating mounting plate (4), wherein the culture partition... (3) has eight sets of annularly distributed culture dish placement holes (34) around its top perimeter. Each of the eight sets of culture dish placement holes (34) is provided with a sealing cap (301) that seals with it. A transfer hole (32) is provided at the center of the top of the culture partition (3). A cell culture dish (35) is placed inside the culture dish placement hole (34). An electric heating plate (41) located at the bottom of the culture dish placement hole (34) and the transfer hole (32) is fixedly installed inside the heating mounting plate (4). The feature is that: Also includes a petri dish conversion mechanism fixedly installed on the top of the inner wall of the top shell (11), which is used to drive the rotating arm (2) to rotate inside the incubator body (1) to determine the position where the culture vessel needs to be converted; The vessel abutting extraction mechanism is slidingly installed inside the rotating arm (2) and is used to abut the culture vessel for extraction and placement operation; The transverse driving mechanism is slidingly installed inside the rotating arm (2) and is used to drive the vessel abutting extraction mechanism to displace; The synchronous flip cover driving mechanism is fixedly installed on the bottom of the rotating arm (2) near one side of the inner wall of the incubator body (1) and is used to follow the rotation of the rotating arm (2) while driving the sealing cover (301) to flip open; The vessel abutting extraction mechanism includes an electric push rod one (223), a lifting rod (224), a fixed frame (2241), an electric push rod two (2242), an abutting plate (2243), and an expansion assembly, the electric push rod one (223) is slidingly installed inside the rotating arm (2), the lifting rod (224) is fixedly installed on the output end of the electric push rod one (223), the fixed frame (2241) is sleeved and fixed on the top of the surface of the lifting rod (224), the electric push rod two (2242) is fixedly installed on the side of the fixed frame (2241) away from the lifting rod (224), the abutting plate (2243) is slidingly sleeved on the bottom of the surface of the lifting rod (224), the top of the abutting plate (2243) is fixedly installed with the output end of the electric push rod two (2242), and the expansion assembly is arranged at the bottom of the lifting rod (224) and is used to tension the cell culture dish (35); The synchronous flip cover driving mechanism includes an annular jacking guide frame (31), a limiting cylinder (21), a sliding rod (211), a roller (212), an arc-shaped tooth plate (213), a spring one (214), and a power conversion flip cover assembly, the annular jacking guide frame (31) is fixedly installed on the top of the culture partition plate (3), the limiting cylinder (21) is fixedly installed on the bottom of the rotating arm (2) near one side of the inner wall of the incubator body (1), the sliding rod (211) is slidingly installed inside the limiting cylinder (21), the roller (212) is rotatably installed at the bottom of the sliding rod (211) through a shaft pin, the arc-shaped tooth plate (213) is fixedly installed at the bottom of the sliding rod (211) near one side of the culture partition plate (3), the spring one (214) is arranged inside the limiting cylinder (21), the top of the spring one (214) is fixedly installed with the top of the inner wall of the limiting cylinder (21), the bottom of the spring one (214) is fixedly installed with the top of the sliding rod (211), and the power conversion flip cover assembly is arranged on the culture partition plate (3) and is arranged with eight groups of annular distribution on the side of the culture dish placement hole (34) away from the transfer hole (32), the power conversion flip cover assembly is used to convert the force of the rotating arm (2) to flip open the sealing cover (301) to facilitate the taking and placing of the cell culture dish (35).

2. The culture device for stem cells according to claim 1, wherein: The culture dish conversion mechanism comprises a top frame (201), a reinforcing disc (202), a stable frame (203) and a servo motor (204), the top frame (201) is arranged on the top of the inner wall of the top shell (11) in four groups and in a ring shape, and is fixedly installed with the top shell (11), the reinforcing disc (202) is fixedly installed at the bottom of the four groups of top frames (201), the stable frame (203) is rotatably installed at the bottom of the reinforcing disc (202), the servo motor (204) is fixedly installed at the center of the top of the inner wall of the top shell (11), and the output end of the servo motor (204) penetrates through the reinforcing disc (202) and extends to the top of the stable frame (203) and is fixedly installed with the stable frame (203).

3. The culture device for stem cells according to claim 1, wherein: The expansion assembly comprises a abutting ring (2244), a connecting ring (22441), a push ring frame (22442), a scissor type telescopic frame (22443), a fixed seat (22444), a tensioning plate (22445) and a spring two (22446), the abutting ring (2244) is slidably installed on the surface of the lifting rod (224) and is fixedly installed with the bottom of the abutting plate (2243), the connecting ring (22441) is detachably installed on the bottom of the abutting ring (2244) through bolts, the push ring frame (22442) is rotatably sleeved on the top of the outer surface of the connecting ring (22441), the scissor type telescopic frame (22443) is provided in three groups, and the top of the side, close to the push ring frame (22442), of the three groups of scissor type telescopic frames (22443) is hinged to the push ring frame (22442), the top end of the side, away from the push ring frame (22442), of the scissor type telescopic frame (22443) is slidably installed with the tensioning plate (22445), the bottom of the side, away from the push ring frame (22442), of the scissor type telescopic frame (22443) is fixedly hinged to the bottom of the side, close to the lifting rod (224), of the tensioning plate (22445), the bottom of the side, close to the lifting rod (224), of the scissor type telescopic frame (22443) is hinged to the fixed seat (22444), and the spring two (22446) is sleeved on the surface of the lifting rod (224), the top of the spring two (22446) is in contact with the bottom of the connecting ring (22441), and the bottom of the spring two (22446) is fixedly installed with the top of the fixed seat (22444).

4. The culture device for stem cells according to claim 1, wherein: The transverse driving mechanism comprises a linear motor stator (22), a linear motor mover (221) and a driving frame (222), the linear motor stator (22) is fixedly installed on the back of the rotating arm (2), the linear motor mover (221) is installed on the surface of the linear motor stator (22), and the driving frame (222) is fixedly installed on the top of the linear motor mover (221).

5. The culture device for stem cells according to claim 1, wherein: The power conversion flip cover assembly comprises a support frame (341), a vertical rod (342), a driving gear one (343), a bevel gear one (345), a bevel gear two (346), a transverse driving rod (347), a driving gear two (348), a driving gear three (349) and a bracket (344), the support frame (341) is fixedly installed on the top of the culture partition (3) away from the transfer hole (32) and close to one side of the sealing cover (301), the vertical rod (342) is longitudinally rotatably installed on one side of the top of the support frame (341), the driving gear one (343) is fixedly installed on the top of the vertical rod (342) and engaged with the arc-shaped toothed plate (213), the bevel gear one (345) is fixedly installed on the bottom of the surface of the vertical rod (342), the transverse driving rod (347) is transversely rotatably installed in the support frame (341), the bevel gear two (346) is fixedly installed on one side of the transverse driving rod (347) close to the bevel gear one (345) and engaged with the bevel gear one (345), the driving gear two (348) is fixedly installed on one side of the transverse driving rod (347) away from the bevel gear two (346), the bracket (344) is fixedly installed on the top of the culture partition (3) between the support frame (341) and the sealing cover (301) on both sides, the driving gear three (349) is fixedly installed on one side of the sealing cover (301) close to the driving gear two (348), the driving gear three (349) is engaged with the driving gear two (348), and the sealing cover (301) is rotatably connected with the bracket (344) through an axle pin on one side close to the support frame (341).

6. The culture device for stem cells according to claim 1, wherein: The bottom of one side of the incubator body (1) is detachably provided with an inspection plate (13), and the four corners of the inspection plate (13) are fixedly connected with the incubator body (1) through magnetic blocks.

7. The culture device for stem cells according to claim 1, wherein: The bottom of the sealing cover (301) is fixedly provided with a temperature sensor (3011), the inside of the culture dish placing hole (34) and the transfer hole (32) are fixedly provided with limiting convex rings (33), and the cell culture dish (35) is longitudinally limited and matched.

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