Cell culture device for cancer cell irradiation experiment

Abstract

The invention belongs to the technical field of medical experiments, and particularly discloses a cell culture device for cancer cell irradiation experiments, which comprises a culture container, and culture holes are formed in the culture container and used for culturing cancer cells. The cell culture device further comprises a base and an irradiation assembly, the number of the culture containers is at least one, the culture containers and the base form a culture part, the irradiation assembly and the base form an irradiation part, and the side edge of the irradiation part is connected with the side edge of the culture part. According to the invention, based on the base, the culture container and the irradiation assembly, the arrangement of the irradiation group in the cancer cell culture process is realized, the requirement of continuously irradiating the cultured cancer cells by radioactive particles during an irradiation experiment can be met, and the irradiation experiment device has relatively high flexibility. In addition, according to the irradiation assembly, the partition plate is matched with the radiation column to form a radiation channel from the radiation source, and orderly and regular irradiation on cancer cells is achieved to meet the irradiation experiment requirement.

Description

Technical Field

[0001] The invention belongs to the technical field of medical experiments, and in particular relates to a cell culture device for cancer cell irradiation experiments. Background Art

[0002] As the incidence of tumors increases year by year, traditional treatment methods (surgery, external radiotherapy, chemotherapy) face multiple difficulties in clinical practice, such as the limitations of surgical resection: some tumors are located in special locations (such as the pancreas, mediastinum) or invade important structures, and the surgical risk is high; the postoperative recurrence rate is high (such as glioma, liver cancer), and the secondary operation is difficult. Radioactive particle implantation therapy technology has become an indispensable new method in the comprehensive treatment of tumors due to its "targeted, continuous, and minimally invasive" characteristics. It is to accurately implant radioactive particles (such as iodine-125 particles, palladium-103 particles, iridium-192 particles) into the tumor, and the low-energy gamma rays continuously emitted by them can effectively kill tumor tissue.

[0003] Clinical treatment has proved that radioactive particle implantation technology has clinical value in solving the pain points of traditional treatment. There are many clinical cases. For example, in case 1, a patient with pancreatic cancer liver metastasis had no pain relief after external radiotherapy. Radioactive particle implantation was used to directly target the lesion. After 3 months, CT showed that the tumor had shrunk by 30%, and the pain score dropped from 8 points to 2 points. Another example is a prostate cancer patient in case 2, a 72-year-old male, diagnosed with early prostate cancer and coronary heart disease. Traditional surgery requires general anesthesia and may damage the urethral sphincter. Radioactive particle implantation is completed under local anesthesia, and the urination function is intact after surgery. It can be seen that radioactive particle implantation treatment technology has developed from a single treatment method to a core component of comprehensive tumor treatment. With the development of medical technology, its application scope will be further expanded to complex scenarios such as oligometastatic cancer and microlesions. In the future, large-scale experimental studies need to be conducted to verify the long-term efficacy and promote the technology to leap in the direction of "precision, intelligence, and individualization" to promote the development and application of radioactive particle implantation treatment technology and provide better treatment options for tumor patients.

[0004] The cancer cell irradiation experiment is a type of experimental research that uses various types of radiation to irradiate cancer cells. It aims to study the mechanism of action of radiation on cancer cells and explore new methods for treating cancer. The cancer cell irradiation experiment uses radioactive particles as the radiation source, which can effectively provide data support for promoting the development and application of radioactive particle implantation therapy technology.

[0005] In the prior art, cancer cell irradiation experiments are conducted by placing cancer cells in a cell culture container, such as a culture container, a cell culture plate, or a cell culture bottle, which is filled with culture fluid. The cell culture container is then cultured using the temperature and other environments required for cancer cell growth in the cell culture box. The cancer cells are then irradiated using an irradiation instrument to achieve the purpose of the experiment. However, the cell culture container is not suitable for irradiation experiments using radioactive particles as a radiation source. This is mainly because radioactive particles are a short-range, low-dose radiation source. Compared with other traditional radiotherapy rays, they require continuous radiation to the cancer cells in order to inhibit the growth of cancer cells. Traditional cancer cell culture containers cannot meet the need for continuous irradiation of cancer cells in culture with radioactive particles. Summary of the invention

[0006] In view of the above problems, the object of the present invention is to provide a cell culture device for cancer cell irradiation experiments, which can meet the demand for continuous irradiation of cancer cells in culture with radioactive particles.

[0007] The technical solution of the present invention is: a cell culture device for cancer cell irradiation experiment, comprising a culture container, the culture container is provided with culture holes, and the culture holes are used to culture cancer cells.

[0008] The cell culture device also includes a base and an irradiation component. There are multiple bases, each of which includes a substrate and a square frame. The cross section of the substrate is a regular n-gon structure, there are n square frames, 3≤n≤6, the n square frames are all arranged on the substrate and are distributed one-to-one above the n side edges of the substrate, the n square frames form a placement groove on the substrate, there is at least one culture container, which is stuck in the placement groove, and the culture container and the base constitute a culture part. There is at least one group of irradiation components, which are stuck in the placement groove, the irradiation components and the culture container are stuck in different placement grooves, the irradiation components are used to place radioactive particles, the irradiation components and the base constitute an irradiation part, and any side of the irradiation part is connected to at least one of the n side edges of the culture part.

[0009] The structure of the base plate and the square frame determines that the base as a whole is a positive n-side column structure. In actual use, the culture part and the irradiation part can be connected in a side-aligned contact arrangement, and the culture part and the irradiation part can be flexibly combined according to experimental requirements. Experimenters can adjust the number and connection method of the culture part and the irradiation part according to different experimental plans, and build a variety of structures similar to cell culture plates to meet the requirements of various complex experiments. The base with a positive n-side column structure can be closely arranged when spliced, effectively utilizing the space in the incubator. This design can accommodate more culture parts and irradiation parts in a limited space, improving the throughput and efficiency of the experiment.

[0010] The culture container and the irradiation component can be stuck in the placement slot. This standardized placement slot design also facilitates the replacement of culture containers and irradiation components of different specifications, improving the versatility and adaptability of the device. In addition, this design ensures the close combination and stability between the components.

[0011] Radioactive particles are placed inside the irradiation component, which can irradiate cancer cells at close range and with precision. The rays released by the radioactive particles can act on cancer cells, simulating the effects of clinical radiotherapy, which helps experimenters gain a deeper understanding of the mechanism and impact of radiotherapy on cancer cells.

[0012] Since the structural design of the device and the installation method of the components are standardized and stable, the operation and conditions during the experiment are easy to control, thereby improving the repeatability of the experiment. Different experimenters can use the device to conduct experiments under the same experimental conditions and obtain relatively consistent results, which enhances the reliability and scientificity of the experimental data.

[0013] Furthermore, n=6, and a plurality of bases form a honeycomb structure.

[0014] On the one hand, multiple bases can be tightly and seamlessly spliced ​​to form a honeycomb structure. This arrangement allows the bases to support and restrict each other, enhancing the stability of the entire structure. During the experiment, even if it is disturbed by certain external forces, such as slight vibrations of the incubator, the overall structure can maintain a relatively stable state, reducing the displacement and damage of the culture container or irradiation component caused by structural instability, thereby ensuring the normal progress of the experiment. From a mechanical point of view, the honeycomb structure has good compression and deformation resistance. The shape of the regular hexagon can evenly disperse stress when subjected to external force, so that the entire structure can withstand greater pressure without deformation. This is especially important for cell culture devices placed in incubators, because there may be certain airflow and temperature changes in the incubator, which may exert certain pressure on the overall structure, and the design of the honeycomb structure can effectively resist the influence of these external forces.

[0015] On the other hand, the honeycomb structure is an arrangement with extremely high space utilization. Compared with other polygonal structures, regular hexagons can fill the plane space to the maximum extent when spliced, reducing space waste. In the limited incubator space, more bases can be placed, thereby increasing the number of culture containers and irradiation components and improving the throughput of the experiment, which means that experimenters can process more samples at the same time in one experiment, greatly improving the efficiency of the experiment. In actual experiments, this structure is also convenient for the reasonable layout of the culture part and the irradiation part. Due to the symmetry of the regular hexagon, experimenters can more easily adjust the position and connection method of the culture part and the irradiation part, making the distance and angle between them more uniform and reasonable, thereby providing a more consistent culture and irradiation environment for cancer cells and improving the accuracy and comparability of experimental results.

[0016] The honeycomb structure makes the environment around each culture container more uniform. During the culture process, the gas exchange is more uniform, which can provide cancer cells with a more stable and consistent oxygen supply, which is conducive to the growth and reproduction of cancer cells. At the same time, during the irradiation process, since the arrangement of the irradiation part and the culture part is more regular and uniform, the rays released by the radioactive particles can irradiate the cancer cells more evenly, reducing the experimental error caused by uneven irradiation and improving the reliability of the experimental results. Multiple culture parts and irradiation parts are closely arranged in the honeycomb structure, which is convenient for studying the interaction between cancer cells and between cancer cells and the irradiation environment. Experimenters can more easily observe the growth and mutual influence of cancer cells in adjacent culture containers, and conduct in-depth research on the group behavior of cancer cells and the influence mechanism of the microenvironment on cancer cells, providing richer experimental data for cancer research.

[0017] Furthermore, the irradiation assembly includes a radiation column and a partition plate, a cavity is provided inside the radiation column, the cavity is used to place the radiation particles, there are 6 partition plates, the 6 partition plates are distributed circumferentially along the axis of the radiation column, one end of the partition plate is arranged on the radiation column, and the other end is clamped on the vertical side of the square frame, the partition plate is made of radiation-proof material, two adjacent partition plates constitute a radiation channel, the starting point of the radiation channel is the cavity, and the end point of the radiation channel is the square frame, and the radiation particles are used as radiation sources along the radiation channel and through the culture part adjacent to the radiation channel to irradiate the cancer cells in the culture part. Through the 6 radiation-proof partition plates distributed circumferentially along the axis of the radiation column, multiple radiation channels are formed, and the rays released by the radiation particles can be directed to irradiate the cancer cells in the culture container along a specific radiation channel. This directional radiation method can accurately control the direction and range of the radiation, so that the cancer cells can receive a more accurate radiation dose, avoid the influence of the scattering of the rays on the surrounding irrelevant areas, and improve the pertinence and effectiveness of the radiation. The design of multiple radiation channels helps to make the radiation dose more evenly distributed in the culture container. Since the starting point of each radiation channel is the cavity of the radiation column and the end point is the square frame, the rays can irradiate cancer cells from multiple directions at the same time, reducing the dose difference caused by a single radiation angle, thereby providing a more uniform radiation environment for cancer cells and enabling the experimental results to more accurately reflect the impact of radiation on cancer cells.

[0018] Furthermore, the radiating column, the partition plate and the square frame are of the same height. This structural design can effectively ensure the high consistency of the overall structure and facilitate use. On the other hand, the high consistency can make the connection between the radiating column, the partition plate and the square frame more stable.

[0019] Furthermore, the irradiation assembly also includes a weakening member, which is a tubular structure, arranged coaxially with the radiation column, and an annular groove is provided at the bottom of the placement groove. The bottom of the weakening member is inserted into the annular groove, and 6 vertical grooves are opened on the weakening member along the circumference of the tube axis, and the vertical grooves are flush with the placement grooves. 6 partition plates are arranged in the vertical grooves one by one. The 6 vertical grooves opened on the weakening member along the circumference of the tube axis provide precise installation positions and guides for the partition plates. The partition plates can be arranged in the vertical grooves one by one, ensuring that the circumferential distribution of the partition plates around the radiation column is uniform and accurate. This makes the shape and size of the radiation channel more regular and consistent, which is conducive to the propagation of rays in a predetermined direction and range, and improves the uniformity and controllability of the radiation. The vertical groove plays a limiting role on the partition plate, which can effectively prevent the partition plate from shifting horizontally or vertically during the experiment. Even if it is subjected to a certain external force, the partition plate will be restricted to a specific position by the vertical groove, ensuring the stability and integrity of the radiation channel, thereby maintaining the consistency of the radiation environment.

[0020] The vertical groove divides the part of the weakening member located in the placement groove into 6 arc-shaped plates, each of which is provided with a hollow cavity, and a weakening layer is placed in the hollow cavity, and the weakening layer is used to weaken the radiation passing through the radiation channel. Different cancer cell experiments may require radiation of different intensities to observe their reactions. By setting a weakening layer, the experimenter can select materials with different weakening capabilities as the weakening layer according to the specific experimental needs, so as to flexibly adjust the radiation dose that passes through the radiation channel and irradiates the cancer cells. For example, for some cancer cell lines that are more sensitive to radiation, a weakening layer with a stronger weakening ability is used to reduce the radiation intensity, avoid excessive damage to cells due to excessive radiation doses, and fail to accurately observe the normal response of cells; for cancer cells with strong radiation tolerance, a weakening layer with a weaker weakening ability can be selected to provide sufficient radiation dose to study the resistance mechanism of cells.

[0021] Furthermore, the weakening layer includes a cavity shell and a particle layer. The cavity shell is inserted in the hollow cavity, and the particle layer is obtained by filling particles in the cavity shell, and is used to weaken the rays passing through the radiation channel; wherein the particles are particles with radiation absorbing materials. In actual use, the weakening ability of the weakening layer is adjusted by deploying different volume percentages of radiation protection material particles.

[0022] Furthermore, the base also includes a blocking part, which includes an upper cover plate and a side cover plate. The upper cover plate corresponds to the structure of the base plate and is made of radiation-proof material. The upper cover plate is placed above the placement slot to absorb radiation passing through the placement slot. The side cover plate corresponds to the structure of the square frame and is made of radiation-proof material. The side cover plate is arranged on the square frame and is located on a side of the square frame away from the placement slot to absorb radiation passing through the square frame. In actual use, the experimenter can selectively use the blocking part according to the experimental requirements to avoid radiation leakage.

[0023] Furthermore, a plurality of clasps are sequentially arranged inside the cavity from top to bottom, and a placement plate is clamped on each clasp, and at least one of the radioactive particles is placed on the placement plate. Multiple clasps can be arranged on placement plates from top to bottom, and radioactive particles can be placed on each placement plate. In actual use, the experimenter can flexibly adjust the spatial distribution of the radiation dose by placing different numbers of radioactive particles on placement plates of different heights according to the experimental requirements. For example, if you want to simulate the difference in radiation dose received by tumor tissues at different depths, you can place fewer particles on the upper placement plate and more particles on the lower placement plate, so as to more accurately study the response of cancer cells to different doses of radiation.

[0024] Furthermore, the clamping ring is an annular structure, and the width of the multiple clamping rings increases from top to bottom. The placement plate is a disc-shaped structure, and the disc diameter of the placement plate decreases from top to bottom. The placement plate is provided with a slot, and the radioactive particles are clamped in the slot.

[0025] Compared with the prior art, the beneficial effect of the present invention is that the present invention realizes the arrangement of the irradiation group during the cancer cell culture process based on the base for the culture container and the irradiation component, wherein the structure of the substrate and the square frame determines that the base as a whole is a positive n-side column structure, and the base, the culture container, and the irradiation component are respectively matched to form the culture part and the irradiation part, and the culture part and the irradiation part can be spliced ​​and flexibly combined according to the experimental requirements, and the requirements of continuous irradiation of the cultured cancer cells by the radioactive particles can be met during the irradiation experiment, and it has high flexibility. In addition, the irradiation component of the present invention cooperates with the radiation column through the dividing plate to realize the formation of the radiation channel from the radiation source, so as to realize the orderly and regular irradiation of the cancer cells to meet the requirements of the irradiation experiment. BRIEF DESCRIPTION OF THE DRAWINGS

[0026] Figure 1 It is a schematic diagram of a partial structure of Embodiment 1 of the present invention;

[0027] Figure 2 It is a schematic diagram of the structure of the culture part of the present invention;

[0028] Figure 3 is a schematic structural diagram of an irradiation unit in accordance with Embodiment 1 of the present invention;

[0029] Figure 4 is a schematic structural diagram of an irradiation unit in Embodiment 2 of the present invention;

[0030] Figure 5 is a schematic structural diagram of a cell culture device of the present invention;

[0031] Figure 6 It is a schematic structural diagram of another cell culture device of the present invention.

[0032] Among them, 1-culture container, 10-culture part, 2-base, 20-placement groove, 21-base plate, 22-square frame, 3-irradiation component, 30-irradiation part, 31-radiation column, 310-cavity, 3100-clamp ring, 32-dividing plate, 33-weakening member, 330-vertical groove, 331-arc plate, 34-weakening layer. DETAILED DESCRIPTION

[0033] Combine the following Figures 1 to 6, the specific implementation methods of the present invention are described in detail. In the description of the present invention, it should be understood that the terms "center", "upper", "lower", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside" and the like indicate positions or positional relationships based on the positions or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as limiting the present invention.

[0034] The terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Therefore, the features defined as "first" and "second" may explicitly or implicitly include one or more of the features; in the description of the present invention, unless otherwise specified, "plurality" means two or more.

[0035] Example 1

[0036] like Figure 1 The cell culture device for cancer cell irradiation experiment shown comprises a culture container 1, on which culture holes are arranged for culturing cancer cells.

[0037] The cell culture device also includes a base 2 and an irradiation assembly 3. There are multiple bases 2, each of which includes a substrate 21 and a square frame 22. The cross section of the substrate 21 is a regular n-gon structure, and there are n square frames 22, 3≤n≤6, and the n square frames 22 are all arranged on the substrate 21 and distributed one by one above the n side edges of the substrate 21. The n square frames 22 surround a placement groove 20 on the substrate 21, and there is at least one culture container 1, which is clamped in the placement groove 20, such as Figure 2 As shown, the culture container 1 and the base 2 constitute the culture part 10. There is at least one irradiation component 3, which is clamped in the placement slot 20. The irradiation component 3 and the culture container 1 are clamped in different placement slots 20. The irradiation component 3 is used to place the radiation particles, such as Figure 3 As shown, the irradiation assembly 3 and the base 2 form an irradiation portion 30 , and any side edge of the irradiation portion 30 is connected to at least one side edge of the n side edges of the culture portion 10 .

[0038] The culture part 10 and the irradiation part 30 form a structure similar to a cell culture plate. In actual use, the number of the culture part 10 and the irradiation part 30 can be selected according to actual needs. When in use, the cell culture device needs to be placed in an incubator for culture.

[0039] The structure of the base plate 21 and the square frame 22 determines that the base as a whole is a regular n-side column structure. In actual use, the culture part and the irradiation part can be connected in a side-aligned contact arrangement, and the culture part and the irradiation part can be flexibly combined according to experimental requirements. Experimenters can adjust the number and connection method of the culture part 10 and the irradiation part 30 according to different experimental schemes, and build a diverse overall structure to meet the requirements of various complex experiments. The base 2 with a regular n-side column structure can be closely arranged when spliced, effectively utilizing the space in the incubator. This design can accommodate more culture parts 10 and irradiation parts 30 in a limited space, thereby improving the throughput and efficiency of the experiment.

[0040] The culture container 1 and the irradiation component 3 can both be stuck in the placement groove 20. This standardized placement groove design is also convenient for replacing culture containers 1 and irradiation components 3 of different specifications, thereby improving the versatility and adaptability of the device. In addition, this design ensures the close combination and stability between the components. During the culture process, the experimenter can observe the growth of cancer cells in the cell culture device. Since the layout of the culture part 10 and the irradiation part 30 is clear, it is convenient to compare and observe and analyze cells in different areas, thereby improving the efficiency and accuracy of experimental observation.

[0041] Radioactive particles are placed inside the irradiation component 3, which can irradiate cancer cells at close range and accurately. The rays released by the radioactive particles can directly act on cancer cells, simulating the effect of clinical radiotherapy, which helps experimenters to gain a deeper understanding of the mechanism and impact of radiotherapy on cancer cells.

[0042] Preferably, n=6, and multiple bases 2 form a honeycomb structure. On the one hand, the regular hexagonal base 2 can be closely and seamlessly spliced ​​to form a honeycomb-like structure. This arrangement allows the bases 2 to support and restrict each other, thereby enhancing the structural stability of the entire cell culture device. During the experiment, even if it is disturbed by certain external forces, such as slight vibrations of the incubator, the overall structure can maintain a relatively stable state, reducing the displacement and damage of the culture container 1 or the irradiation component 3 caused by structural instability, thereby ensuring the normal progress of the experiment. From a mechanical point of view, the honeycomb structure has good compression and deformation resistance. The shape of the regular hexagon can evenly disperse stress when subjected to external force, so that the entire structure can withstand greater pressure without deformation. This is particularly important for a cell culture device placed in an incubator, because there may be certain airflow and temperature changes in the incubator, which may exert certain pressure on the overall structure, and the design of the honeycomb structure can effectively resist the influence of these external forces.

[0043] On the other hand, the honeycomb structure is an arrangement with extremely high space utilization. Compared with other polygonal structures, regular hexagons can fill the plane space to the maximum extent when spliced, reducing space waste. In the limited incubator space, more bases 2 can be placed, thereby increasing the number of culture containers 1 and irradiation components 3 and improving the throughput of the experiment, which means that the experimenter can process more samples at the same time in one experiment, greatly improving the efficiency of the experiment. In addition, since the radiation radius of the radioactive particles is generally 1.7 cm, that is, its effective radiation distance is 1.7 cm, and beyond this distance, the radiation dose is very small, therefore, placing a large number of irradiation components 3 in the limited incubator space will not cause excessive radiation interference to the cancer cells in the non-adjacent culture containers 1 due to the increase in number of irradiation components 3. In actual experiments, this structure is also convenient for the reasonable layout of the culture part 10 and the irradiation part 30. Due to the symmetry of the regular hexagon, the experimenter can more conveniently adjust the position and connection method of the culture part 10 and the irradiation part 30 to make the distance and angle between them more uniform and reasonable, thereby providing a more consistent culture and irradiation environment for cancer cells and improving the accuracy and comparability of the experimental results.

[0044] The honeycomb structure makes the environment around each culture container 1 more uniform, and during the culture process, the gas exchange is more uniform, which is conducive to the growth and reproduction of cancer cells. At the same time, during the irradiation process, since the arrangement of the irradiation part 30 and the culture part 10 is more regular and uniform, the rays released by the radioactive particles can be irradiated to the cancer cells more uniformly, reducing the experimental error caused by uneven irradiation and improving the reliability of the experimental results. Multiple culture parts 10 and irradiation parts 30 are closely arranged in the honeycomb structure, which is convenient for studying the interaction between cancer cells and between cancer cells and the irradiation environment. Experimenters can more conveniently observe the growth and mutual influence of cancer cells in adjacent culture containers 1, and conduct in-depth research on the group behavior of cancer cells and the influence mechanism of the microenvironment on cancer cells, providing more abundant experimental data for cancer research.

[0045] The regular hexagonal base 2 has obvious symmetry and regularity, making the positions of the culture part 10 and the irradiation part 30 clearer and easier to identify. When the experimenter is performing operations such as cell inoculation, culture fluid replacement, and irradiation component 3 replacement, he can find the target position more accurately and reduce the occurrence of operational errors. At the same time, the layout of the honeycomb structure also makes the operation more convenient. The experimenter can operate each culture container 1 and irradiation component 3 in a certain order, thereby improving the operating efficiency.

[0046] After the experiment, it is more convenient to clean, disinfect and store the cell culture device. Due to the regularity of the honeycomb structure, the connection and disassembly between the bases 2 are easier, and the experimenter can quickly disassemble and assemble the cell culture device, which improves the efficiency of maintenance and management.

[0047] Moreover, the placement groove 20 surrounded by 6 square frames 22 on the substrate 21 is also a regular hexagonal column structure. The regular regular hexagonal column shape makes the installation process of the culture container 1 and the irradiation component 3 simpler and more accurate. The experimenter does not need to spend extra energy to adjust the direction and position, and only needs to put the components into the placement groove 20, which improves the efficiency of the experimental operation to a certain extent, especially when a large number of components need to be installed. The advantage is more obvious. Moreover, the placement groove 20 of the regular hexagonal column can make full use of the internal space of the base and avoid space waste. Compared with placement grooves of other shapes, the regular hexagonal placement groove 20 can be arranged more closely in the regular hexagonal column base 2, so that the internal space of the base 2 is maximized, and then under the same base size, a larger volume or more number of culture containers 1 and irradiation components 3 can be accommodated, which improves the culture and irradiation capacity of the entire device. Most importantly, for the irradiation component 3, the regular hexagonal column placement groove 20 is conducive to the uniform distribution of the rays released by the radiation particles. The radiation can irradiate the cancer cells in the culture container 1 in a relatively uniform manner, reducing the experimental deviation caused by uneven irradiation, so that the experimental results can more accurately reflect the real response of the cancer cells under specific irradiation conditions, and enhancing the reliability and scientificity of the experimental data.

[0048] like Figure 5 In the cell culture device shown in FIG. 1 , the cancer cells placed in the culture part 10 are irradiated by only the radiation particles of one irradiation part 30. Figure 6 In the cell culture device shown in FIG. 1 , the culture part 10 is irradiated by the radiation particles of the surrounding n irradiation parts 30, and the radiation amount is relatively Figure 5 When studying the effects of different irradiation doses on cancer cells, the experimenter can adjust the irradiation dose of the culture part 10 by increasing or decreasing the number of irradiation parts 30. In addition, by adjusting the connection mode and position relationship between the irradiation part 30 and the culture part 10, different irradiation conditions and microenvironments can be simulated, providing an experimental basis for the optimization of clinical radiotherapy plans.

[0049] Preferably, Figure 3As shown, the irradiation assembly 3 includes a radiation column 31 and a partition plate 32. A cavity 310 is provided inside the radiation column 31, and the radiant particles are placed in the cavity 310. There are 6 partition plates 32, and the 6 partition plates 32 are distributed circumferentially along the axis of the radiation column 31. One end of the partition plate 32 is set on the radiation column 31, and the other end is clamped on the vertical side of the square frame 22. The partition plate 32 is made of radiation-proof material. Two adjacent partition plates 32 form a radiation channel. The starting point of the radiation channel is the cavity 310, and the end point of the radiation channel is the square frame 22. The radiant particles are used as radiation sources along the radiation channel and through the culture part 10 adjacent to the radiation channel to irradiate the cancer cells in the culture part 10. In this embodiment, the partition plate 32 is specifically made of lead glass.

[0050] Six radiation-proof partition plates 32 are distributed circumferentially along the axis of the radiation column 31 to form multiple radiation channels. The radiation released by the radiation particles can be directed along a specific radiation channel to irradiate the cancer cells in the culture container 1. This directional radiation method can accurately control the direction and range of the radiation, so that the cancer cells can receive a more accurate radiation dose, avoid the influence of the scattering of the radiation on the surrounding irrelevant areas, and improve the pertinence and effectiveness of the radiation. The design of multiple radiation channels helps to make the radiation dose more evenly distributed in the culture container. Since the starting point of each radiation channel is the cavity 310 of the radiation column and the end point is the side of the base 2, the radiation can irradiate the cancer cells from multiple directions at the same time, reducing the dose difference caused by the single radiation angle, thereby providing a more uniform radiation environment for the cancer cells, so that the experimental results can more accurately reflect the effect of radiation on cancer cells.

[0051] This directional radiation design can better simulate the way radiation irradiates tumor tissue in clinical radiotherapy. In actual clinical treatment, radiation oncologists will try their best to accurately irradiate the tumor while reducing damage to surrounding normal tissues. The design of this irradiation component can provide experimenters with a radiation environment that is closer to the actual clinical situation in the laboratory, which is helpful for in-depth research on the mechanism of action and efficacy evaluation of radiotherapy on cancer cells, and provide more valuable experimental basis for the optimization of clinical radiotherapy plans.

[0052] Preferably, the radiation column 31, the partition plate 32 and the square frame 22 are of the same height. This structural design can effectively ensure the high consistency of the overall structure and facilitate use. On the other hand, the high consistency can make the connection between the radiation column 31, the partition plate 32 and the square frame 22 more stable.

[0053] Preferably, a plurality of clamping rings 3100 are sequentially arranged inside the cavity 310 from top to bottom, and a placement plate is clamped on each clamping ring 3100, and at least one radioactive particle is placed on the placement plate.

[0054] Preferably, the clasp 3100 is an annular structure, and the width of the plurality of clasps 3100 increases from top to bottom, the placement plate is a disc-shaped structure, and the disc diameter of the placement plate decreases from top to bottom, and a slot is provided on the placement plate, and the radioactive particles are clamped in the slot. The plurality of clasps 3100 can be arranged in order from top to bottom on the placement plates, and radioactive particles can be placed on each placement plate. In actual use, the experimenter can flexibly adjust the spatial distribution of the radiation dose by placing different numbers of radioactive particles on placement plates of different heights according to the experimental requirements. For example, if you want to simulate the difference in radiation dose received by tumor tissues at different depths, you can put fewer particles on the upper placement plate and more particles on the lower placement plate, so as to more accurately study the response of cancer cells to different doses of radiation.

[0055] Different cancer cell experiments may require different radiation doses and irradiation modes. By adjusting the number and position of the radiation particles on each placement plate, a variety of experimental needs can be met. Whether it is a high-dose short-time irradiation or a low-dose long-time irradiation experiment, it can be easily achieved, which improves the applicability and flexibility of the experimental device.

[0056] Multiple placement plates form multiple radiation source layers in the cavity 310, and the radiation particles release radiation from different heights and positions, so that the radiation is more evenly distributed in the culture container 1. Compared with a radiation source at a single position, this layered layout can reduce radiation blind spots, avoid the accuracy of the experimental results being affected by excessive differences in local radiation doses of cancer cells, and help to more realistically reflect the overall response of cancer cells to radiation.

[0057] Uniform radiation distribution can ensure that cancer cells at all positions in the culture container 1 can receive relatively consistent radiation doses, making the experimental results more reliable and comparable. This is crucial for studying the effects of radiation on cancer cell growth, apoptosis, gene mutation, etc., and provides more accurate experimental data for a deeper understanding of the mechanism of cancer radiotherapy.

[0058] It should be noted that: the radioactive particles in this embodiment use iodine-125 particles. Since the radiation radius of iodine-125 particles is generally 1.7 cm, the radiation dose is very small beyond this distance. Therefore, the diameter of the inscribed circle of the regular n-gon corresponding to the cross section of the base 2 is 1 cm, which effectively ensures that the radioactive particles in the irradiation part 30 can effectively irradiate the cancer cells in the culture part 10. In addition, the substrate 21 and the square frame 22 of this embodiment are an integrated structure, both made of polystyrene, and the two adjacent bases 2 are connected by a clamping connection set on the outer wall of the square frame 22. Since the square frame 22 is a frame structure, Figure 1As shown, the iodine-125 particles inside the irradiation component 3 are used as a radiation source to irradiate cancer cells and are only blocked by the side wall of the culture container 1. Therefore, the culture container 1 is made of polystyrene, and the problem of the radiation weakening of the radiation source by the side wall of the culture container 1 is ignored in actual experiments.

[0059] Example 2

[0060] The difference from Example 1 is that: Figure 4 As shown, preferably, the irradiation assembly 3 further includes a weakening member 33, which is a tubular structure and is coaxially arranged with the radiation column 31. An annular groove is provided at the bottom of the substrate 21, and the bottom of the weakening member 33 is inserted in the annular groove. Six vertical grooves 330 are opened on the weakening member 33 along the circumference of its tube axis. The vertical grooves 330 are flush with the placement groove 20, and six dividing plates 32 are correspondingly penetrated in the vertical grooves 330. The vertical grooves 330 divide the part of the weakening member 33 located in the placement groove 20 into six arc-shaped plates 331, each of which is provided with a hollow cavity, and a weakening layer 34 is placed in the hollow cavity. The weakening layer 34 is used to weaken the radiation passing through the radiation channel.

[0061] This design provides additional support for the entire irradiation assembly 3, making the radiation column 31 more stable in the placement slot 20. During the experiment, external interference may occur due to factors such as the vibration of the incubator and the slight touch of the experimenter, and the weakening member 33 can effectively resist these external forces, prevent the radiation column from shaking or tilting, and ensure that the position of the iodine experimenter-experimenter 125 experimenter particles is relatively fixed, thereby ensuring the stability and accuracy of the radiation.

[0062] The six vertical grooves 330 opened along the circumference of the tube axis on the weakening member 33 provide a precise installation position and guide for the dividing plate 32. The dividing plates 32 can be inserted into the vertical grooves 330 one by one, ensuring that the circumferential distribution of the dividing plates 32 around the radiation column 31 is uniform and accurate. This makes the shape and size of the radiation channel more regular and consistent, which is conducive to the propagation of rays in a predetermined direction and range, and improves the uniformity and controllability of radiation. The vertical grooves 330 limit the dividing plate 32 and can effectively prevent the dividing plate 32 from shifting horizontally or vertically during the experiment. Even if it is subjected to a certain external force, the dividing plate 32 will be restricted to a specific position by the vertical grooves, ensuring the stability and integrity of the radiation channel, thereby maintaining the consistency of the radiation environment.

[0063] Furthermore, when installing the irradiation assembly 3, the vertical groove 330 of the weakening member 33 provides a clear guide for the installation of the partition plate 32, so that the experimenter can more conveniently and quickly install the partition plate 32 accurately to the designated position. Similarly, when disassembling and cleaning operations are performed after the experiment, the partition plate 32 can be taken out of the vertical groove 330 more easily, thereby improving the efficiency of the experimental operation.

[0064] Different cancer cell experiments may require different intensities of radiation to observe their reactions. By setting the weakening layer 34, the experimenter can select materials with different weakening capabilities as the weakening layer 34 according to the specific experimental requirements, so as to flexibly adjust the radiation dose that passes through the radiation channel and irradiates the cancer cells. For example, for some cancer cell lines that are more sensitive to radiation, a weakening layer 34 with a stronger weakening capability is used to reduce the radiation intensity to avoid excessive damage to the cells due to excessive radiation doses, and the inability to accurately observe the normal response of the cells; while for cancer cells with strong radiation tolerance, a weakening layer 34 with a weaker weakening capability can be selected to provide sufficient radiation dose to study the resistance mechanism of the cells.

[0065] During the experiment, precise control of the radiation dose is crucial to obtaining accurate and reliable experimental results. The weakening layer 34 can finely adjust the radiation to reduce the fluctuation and error of the radiation dose. Even if there is a certain instability in the radiation output of the radioactive particles, the weakening layer can also play a role of buffering and regulation, making the radiation dose irradiated to the cancer cells more stable and accurate, thereby improving the repeatability and comparability of the experimental results.

[0066] Preferably, the weakening layer 34 includes a cavity shell and a particle layer. The cavity shell is inserted in the hollow cavity. The particles are particles with radiation absorbing materials, which are used to weaken the rays passing through the radiation channel; wherein the particles include radiation-proof material particles with a volume percentage of V% and non-radiation-proof material particles with a volume percentage of (100-V)%, 100≥V≥1. It should be noted that the radiation-proof material particles can be aluminum powder particles, lead glass particles, etc. In this embodiment, the radiation-proof material particles use aluminum powder particles, and the non-radiation-proof material particles use plastic particles. After the radiation-proof material particles and the non-radiation-proof material particles are mixed evenly, they are filled into the cavity shell to obtain the weakening layer 34. In addition, V does not take a value less than 1, because when V takes a value less than 1, it is assumed that the radiation protection ability of the weakening layer 34 against the radiated particles is very weak, and the weakening layer 34 is not provided. In actual experiments, V often takes values ​​such as 20, 25, 30, ..., 100. It should be noted that: when the value of V is large, the weakening layer 34 reduces the radiation intensity. When the value of V is 100, the default weakening layer 34 weakens the radiation of the radiated particles passing through the radiation channel to 0, which can be selectively used when setting a control group that is not irradiated. Since the theoretical volume percentage V% of the radiation-proof material particles in the particles can be adjusted within the range of 100≥V≥1, the experimenter can accurately control the degree of attenuation of the rays by the weakening layer according to the specific experimental requirements. When a higher radiation dose is required for the experiment, the volume percentage of the radiation-proof material particles can be appropriately reduced or the weakening layer 34 can be not set; when the radiation dose needs to be strictly limited, the percentage can be increased, thereby achieving flexible regulation of the radiation dose and improving the accuracy of the experiment.

[0067] The structure of the particle layer allows the radiation protection material particles to be evenly distributed in the cavity shell. This even distribution helps to absorb and scatter the radiation more evenly, avoiding excessive or insufficient local radiation attenuation. Compared with a single block of radiation protection material, the particle layer can provide a larger surface area to interact with the radiation, thereby more effectively attenuating the radiation and ensuring a more uniform attenuation effect in the entire radiation channel.

[0068] Example 3

[0069] The difference from the second embodiment is that the base 2 further includes a blocking part, which includes an upper cover plate and a side cover plate. The upper cover plate corresponds to the structure of the base plate 21 and is made of radiation-proof material. The upper cover plate is placed above the placement slot 20 to absorb the radiation passing through the placement slot 20. The side cover plate corresponds to the structure of the square frame 22 and is made of radiation-proof material. The side cover plate is arranged on the square frame 22 and is located on the side of the square frame 22 away from the placement slot 20 to absorb the radiation passing through the square frame 22. In actual use, the experimenter can selectively use the blocking part according to the experimental requirements to avoid radiation leakage.

[0070] The specific embodiments described above further illustrate the objectives, technical solutions and beneficial effects of the present invention in detail. It should be understood that the above description is only a specific embodiment of the present invention and does not limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention are included in the protection scope of the present invention.

Claims

1. A cell culture device for cancer cell irradiation experiments, comprising a culture container (1), wherein the culture container (1) is provided with culture holes for culturing cancer cells, wherein the culture holes are used for culturing cancer cells, and wherein: Also includes: There are multiple bases (2), each base (2) comprising: a substrate (21) having a regular n-gonal structure in cross section; There are n square frames (22), 3≤n≤6, the n square frames (22) are arranged on the base plate (21) and are distributed one-to-one above the n side edges of the base plate (21), and the n square frames (22) surround a placement groove (20) on the base plate (21); at least one culture container (1) is clamped in the placement groove (20), and the culture container (1) and the base (2) form a culture part (10); At least one group of irradiation components (3) is clamped in the placement groove (20), the irradiation components (3) and the culture container (1) are clamped in different placement grooves (20), the irradiation components (3) are used to place radioactive particles, the irradiation components (3) and the base (2) form an irradiation part (30), and any side edge of the irradiation part (30) is connected to at least one side edge of the n side edges of the culture part (10).

2. A cell culture device for cancer cell irradiation experiments as claimed in claim 1, characterized in that: The n=6, and the multiple bases (2) form a honeycomb structure.

3. A cell culture device for cancer cell irradiation experiments as claimed in claim 1, characterized in that: The irradiation component (3) comprises: A radiation column (31) having a cavity (310) therein, wherein the cavity (310) is used to place radiation particles; There are six dividing plates (32), and the six dividing plates (32) are distributed circumferentially along the axis of the radiation column (31); one end of the dividing plate (32) is arranged on the radiation column (31), and the other end is clamped on the vertical side of the square frame (22); the dividing plate (32) is made of radiation-proof material; two adjacent dividing plates (32) form a radiation channel; the starting point of the radiation channel is the cavity (310), and the end point of the radiation channel is the square frame (22); the radioactive particles are used as radiation sources to travel along the radiation channel and pass through the culture section (10) adjacent to the radiation channel to irradiate the cancer cells in the culture section (10).

4. A cell culture device for cancer cell irradiation experiment as claimed in claim 3, characterized in that: The radiation column (31), the partition plate (32) and the square frame (22) are of the same height.

5. A cell culture device for cancer cell irradiation experiment as claimed in claim 3, characterized in that: The irradiation assembly (3) further comprises a weakening member (33), the weakening member (33) being a tubular structure and arranged coaxially with the radiation column (31); an annular groove is provided at the bottom of the base plate (21); the bottom of the weakening member (33) is inserted into the annular groove; six vertical grooves (330) are provided on the weakening member (33) along the circumference of the tube axis; the vertical grooves (330) are flush with the placement groove (20); and six partition plates (32) are inserted into the vertical grooves (330) in a one-to-one correspondence; The vertical groove (330) divides the portion of the weakening member (33) located in the placement groove (20) into six arc-shaped plates (331), each of the arc-shaped plates (331) being provided with a hollow cavity, a weakening layer (34) being placed in the hollow cavity, and the weakening layer (34) being used to weaken radiation passing through the radiation channel.

6. A cell culture device for cancer cell irradiation experiments as claimed in claim 5, characterized in that: The weakened layer (34) comprises: A cavity shell is inserted into the hollow cavity; The particle layer is obtained by filling the cavity shell with particles and is used to weaken the rays passing through the radiation channel; wherein the particles are particles containing radiation absorbing materials.

7. The cell culture device for cancer cell irradiation experiment according to claim 1, characterized in that: The base (2) further comprises a blocking portion, which comprises: An upper cover plate, corresponding to the structure of the base plate (21), is made of radiation-proof material, and is placed above the placement slot (20) to absorb radiation passing through the placement slot (20); The side cover plate corresponds to the structure of the square frame (22) and is made of radiation-proof material. The side cover plate is arranged on the square frame (22) and is located on a side of the square frame (22) away from the placement groove (20), and is used to absorb radiation passing through the square frame (22).

8. The cell culture device for cancer cell irradiation experiment according to claim 1, characterized in that: A plurality of clamping rings (3100) are sequentially arranged inside the cavity (310) from top to bottom, and a placement plate is clamped onto each clamping ring (3100), and at least one of the radioactive particles is placed on the placement plate.

9. A cell culture device for cancer cell irradiation experiments as claimed in claim 8, characterized in that: The clamping ring (3100) is an annular structure, and the widths of the multiple clamping rings (3100) increase from top to bottom. The placement plate is a disc-shaped structure, and the disc diameter of the placement plate decreases from top to bottom. The placement plate is provided with a clamping groove, and the radioactive particles are clamped in the clamping groove.

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