High-temperature-resistant auricularia plicata strain and application thereof

Abstract

The invention relates to a high-temperature-resistant auricularia delicata strain Adel 27, which has a preservation number of CCTCC NO: M 2025114, is dense in bacterial colony, long in growth period, high-temperature-resistant, single-piece clustered in sporocarp, small in auricular base, light brown in auricular piece and soft in texture, and can be used for auricularia delicata cultivation and breeding. The cultivated auricularia plicata is high in nutrient substance content, can turn moisture for 3-4 times, can greatly improve the yield and reduce the cost, and is suitable for a factory large-scale production mode of one-time harvesting.

Description

Technical Field

[0001] The invention relates to the biological field, and in particular to a high-temperature-resistant Auricularia auricularia auricula strain and application thereof. Background Art

[0002] Auricularia delicata (Mont.ex Fr.) Henn. is an edible fungus that can be used as both food and medicine. It has the function of improving the body's immune function, has a preventive and therapeutic effect on coronary heart disease, and can also be used as a traditional medicine to treat gastrointestinal and liver diseases. Auricularia delicata is one of the emerging edible fungi industries in my country. It is produced on a large scale in Sichuan, Yunnan, Guizhou, Jilin and other places. It is one of the characteristic edible fungi species in the southwest and northeast regions. Because it has a special mushroom flavor and a taste between meat and gelatin, its fruiting body has reticular wrinkles on the ventral side, which not only increases the layered feeling of eating, but also can retain the flavor substances of cooking ingredients. As a high-quality edible fungus ingredient, it has attracted much attention from consumers, but its nutritional content is low, there are few cultivated varieties of Auricularia delicata, and it is not resistant to high temperatures. Therefore, it is necessary to find strains of Auricularia delicata that can cultivate higher nutritional value and high temperature resistance. Summary of the invention

[0003] The invention aims to provide a wrinkled fungus strain Adel 27 (Auricularia delicata), whose preservation number is CCTCC NO: M 2025114.

[0004] Another object of the present invention is to provide the application of the Auricularia auricula strain of the present invention in the cultivation and breeding of Auricularia auricula.

[0005] According to a preferred technical solution of the present invention, the temperature of the Auricularia auricularia strain during the ear-growing period is 14-30°C.

[0006] According to a preferred technical solution of the present invention, the wrinkled Auricularia auricula obtained by cultivating the wrinkled Auricularia auricula strain has a fresh ear piece having a light brown ventral surface and a gray-brown dorsal surface; a dried ear piece has a yellow-brown ventral surface and a brown dorsal surface.

[0007] According to a preferred technical solution of the present invention, the dry-to-wet ratio of the ear pieces of the wrinkled wood ear is 1:8-1:8.5.

[0008] In a preferred technical solution of the present invention, the crude polysaccharide content of the wrinkled fungus is greater than 10%, preferably greater than 12%, and more preferably greater than 16%.

[0009] According to a preferred technical solution of the present invention, the protein content of the Auricularia auricula is greater than 4%, preferably greater than 5%, and more preferably greater than 6%.

[0010] According to a preferred technical solution of the present invention, the total amino acid content of the Auricularia auricula is greater than 2%, preferably greater than 3%, and more preferably greater than 4%.

[0011] According to the preferred technical solution of the present invention, the Auricularia auricularia strain has good resistance and is free from specific diseases and insect pests.

[0012] According to a preferred technical solution of the present invention, the hardness of the wrinkled fungus is greater than 200, preferably greater than 220, and more preferably greater than 240.

[0013] According to a preferred technical solution of the present invention, the elasticity of the wrinkled wood ear is greater than 0.4, preferably greater than 0.6, and more preferably greater than 0.8.

[0014] Unless otherwise specified, when the present invention relates to the percentage between liquids, the percentage is volume / volume percentage; when the present invention relates to the percentage between liquids and solids, the percentage is volume / weight percentage; when the present invention relates to the percentage between solids and liquids, the percentage is weight / volume percentage; the rest are weight / weight percentages.

[0015] The present invention adopts the following detection method to detect:

[0016] 1. Dry-wet ratio: refers to the ratio of the dry weight of the ear piece to the wet weight after soaking. The wet weight test method is to soak the dry ear in water at room temperature for 4 hours, take it out and drain it, use moisture-absorbing paper to absorb the moisture on the surface of the ear piece, and weigh it, refer to "NY / T 3729-2020 Plant Variety Specificity (Distinguishability), Consistency and Stability Testing Guide"

[0017] 2. Amino acid: GB 5009.124-2016; protein: GB 5009.5-2016 Method 1; crude polysaccharide: NY / T1676-2023.

[0018] 3. Hardness and elasticity: The total texture analysis (TPA) method of the texture analyzer is used, with a pre-test speed of 1mm / s, a test speed of 1mm / s, a post-test speed of 1mm / s, a target mode of compression (strain), a deformation of 50%, and a trigger force of 5g. A cylindrical P75 probe (75mm in diameter) is used.

[0019] Compared with the prior art, the beneficial technical effects of the present invention include:

[0020] The wrinkled wood ear strain scientifically screened by the invention has dense colonies, a long growth period (ears can be grown from May to September), high temperature resistance (ears can be grown at 30 DEG C), and the cultivated wrinkled wood ear has high nutrient content (polysaccharide content is twice that of ordinary wrinkled wood ear), soft texture, neat ears, and can be tidal 3-4 times, which can greatly increase the yield (twice that of ordinary wrinkled wood ear). At the same time, the adaptability to raw materials is wide (ordinary agricultural waste materials can be cultivated by formula), the cost is low, and the factory-based large-scale production mode suitable for one-time harvesting is broad market prospects.

[0021] Biomaterial Deposit

[0022] The wrinkled fungus strain described in the present invention is named as wrinkled fungus Adel 27 (Auricularia delicata), which was submitted for preservation on January 13, 2025, with the preservation number: CCTCC NO: M2025114, the preservation unit: China Center for Type Culture Collection (CCTCC), and the preservation address is Luojia Mountain, Bayi Road, Wuchang District, Wuhan City, Hubei Province. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 The front side of the Auricularia auricularia mycelium of the present invention;

[0024] Figure 2 The mycelium of Auricularia auricula of the present invention (cultivar);

[0025] Figure 3 The invention discloses the ventral surface of the fruiting body of Auricularia auricula. DETAILED DESCRIPTION

[0026] The present invention will be described below with reference to examples, but the present invention is not limited to the examples.

[0027] Example 1 Screening of Auricularia auricula strains of the present invention

[0028] The wild Auricularia delicata fruiting bodies collected from Muchuan County, Leshan City, Sichuan Province were separated from wild material tissues to obtain wild material strains, which were then purified by pure culture. The Auricularia delicata strain of the present invention was obtained by a systematic breeding method. The strain was identified as Auricularia delicata (Fr.) Henn. by morphological and ITS sequence analysis. The basidiospores of the strain are elliptical to oval, the hyphae are translucent and filamentous, and have lock-like joints ( Figure 1-2 ).

[0029] The wrinkled fungus strain described in the present invention is named Adel 27 (Auricularia delicata), which was submitted for preservation on January 13, 2025, with the preservation number: CCTCC NO: M 2025114, the preservation unit: China Center for Type Culture Collection (CCTCC), and the preservation address is Luojia Mountain, Bayi Road, Wuchang District, Wuhan City, Hubei Province.

[0030] Example 2 Field test study of the Auricularia auricula strain of the present invention

[0031] The field experiment was conducted in a greenhouse of an experimental base in Shifang City, Sichuan Province. The wrinkled Auricularia auricularia strain of the present invention was used to conduct a variety regional test for two consecutive years.

[0032] Potato dextrose agar medium (PDA): contains 200g potato, 20g glucose, 20g agar and 1000mL distilled water; take 20mL of the medium and put it into a test tube (test tube diameter 20mm×200mm), sterilize at 121℃ for 30min, cool, and you will get a test tube filled with PDA solid medium.

[0033] Preparation of Auricularia auricularia mycelium culture: The Auricularia auricularia strain Adel27 of the present invention is placed in a test tube (test tube diameter 20 mm×200 mm) containing a PDA solid culture medium, and cultured at 25° C. for 15 days to obtain the culture.

[0034] A cultivation bag (200 mm × 480 mm × 0.28 mm) containing 1000 g of cultivation material: the composition of the cultivation material by weight is 330 g of sawdust, 100 g of cottonseed hulls, 300 g of corn cobs, 200 g of wheat bran, 20 g of corn flour, 40 g of lime, 10 g of gypsum and 600 g of water. The cultivation bag is sterilized at 121°C for 2.5 hours and then cooled for use.

[0035] Field experiment: Shelf-type bag planting, no less than 60 bags, with 3 replicates. Bags are made in December, and ears are produced from May to September of the following year. The cultivation bags with an inoculation amount of 10g of Auricularia auricularia mycelium culture / bag are placed in a culture room at 18-26℃ and protected from light for 30 days. After the fungus is completed, ear production management is carried out. The temperature during the ear production period is 14℃~30℃, and the relative humidity of the air in the ear shed is maintained at 85%~95%; the light intensity should not exceed 310 lux on sunny days, and the ventilation is good. The shed door is opened for ventilation in the morning and evening, and the ventilation time is 15-30 minutes / day. The edges of the fruiting body ear pieces are slightly upturned and can be harvested.

[0036] The cultivation results show that the yield is 88.6g / bag in dry weight and 708.8g / bag in fresh weight. The mature fruiting bodies are single-piece clustered, with smooth edges. The ventral surface of the fresh ear is covered with grid-like wrinkles and is light brown, while the back is gray-brown. The ventral surface of the dry ear is yellow-brown and the back is brown ( Figure 3 ); When the ears come out from both ends of the long stick cultivation in the shed, the length of the fresh ears is 69.3-79.4 mm, the width of the ears is 90.3-116.8 mm, the thickness of the ears is 2.0-2.8 mm, and the dry-wet ratio of the ears is 1:8.

[0037] Routine nutrient composition determination of mature fruiting body dried ear slices showed that each 100 grams contained 16.7 grams of crude polysaccharide, 6.88 grams of protein, and 4.83 grams of total amino acids. The texture was soft, with a hardness value of 245.93±35.419 and an elasticity value of 0.82±0.056.

[0038] In addition, statistics show that the contamination rate of the cultivated species of Auricularia auricula mycelium is only 2.3%, and the infected bacteria are common edible fungi green mold in production, and the infection is caused by the damage of the mushroom sticks, and no specific diseases and insect pests occur during the ear-growing period. The strain of the present invention has strong resistance.

[0039] The above description of the specific embodiments of the present invention does not limit the present invention. Those skilled in the art may make various changes or modifications based on the present invention. As long as they do not depart from the spirit of the present invention, they should all fall within the scope of protection of the claims of the present invention.

Claims

1. A strain of Auricularia delicata Adel 27, with a preservation number of CCTCCNO: M 2025114.

2. Use of the Auricularia auricula strain as claimed in claim 1 in cultivation and breeding of Auricularia auricula.

3. The use according to claim 2, wherein the temperature during the growth period of the Auricularia auricularia auricularia strain is 14-30°C.

4. The use according to any one of claims 2-3, wherein the Auricularia auricula cultivated with the Auricularia auricula strain has a fresh ear whose ventral surface is light brown and whose back is gray-brown; a dried ear whose ventral surface is yellow-brown and whose back is brown.

5. The use according to any one of claims 2 to 4, wherein the dry-to-wet ratio of the ear pieces of the Auricularia auricula is 1:

8.

6. The use according to any one of claims 2 to 5, wherein the crude polysaccharide content of the Auricularia auricula is greater than 10%, preferably greater than 12%, and more preferably greater than 16%.

7. The use according to any one of claims 2 to 6, wherein the protein content of the Auricularia auricula is greater than 4%, preferably greater than 5%, and more preferably greater than 6%.

8. The use according to any one of claims 2 to 7, wherein the total amino acid content of the Auricularia auricula is greater than 2%, preferably greater than 3%, and more preferably greater than 4%.

9. The use according to any one of claims 2 to 8, wherein the hardness of the Auricularia auricula is greater than 200, preferably greater than 220, and more preferably greater than 240.

10. The use according to any one of claims 2 to 9, wherein the elasticity of the Auricularia auricula is greater than 0.4, preferably greater than 0.6, and more preferably greater than 0.8.

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