Device and method for labeling carbon and nitrogen isotopes of plants

Abstract

The invention provides a plant carbon and nitrogen isotope labeling device which comprises a pool body, a double-layer support, an outer cover shell, a labeling pipe, a fan, a sucking pump and a recorder, the double-layer support is arranged in the middle in the pool body, the recorder is placed in the middle of the double-layer support, a plant is planted in a plant platform at the top end of the double-layer support, and the outer cover shell is arranged on the outer cover shell. A marking pipe used for marking 15N is further arranged on the plant platform, the double-layer support, the plant platform and the plants are surrounded by the outer cover shell, a fan is arranged in the middle of the outer top of the outer cover shell, an air pump is further arranged on one side of the outer cover shell, and an injector is further communicated with the outer cover shell. The 15N isotope leaf labeling method is simple and easy to implement, compared with a 15N isotope soil labeling method adopted in the prior art, leaf nitrogen absorption cannot be diluted by a matrix in the labeling process, the plant 15N labeling effect is better, two elements of C and N are considered, and the method is suitable for large-scale popularization and application. And the carbon and nitrogen transfer amount, direction and coupling relationship among the organs of the plant can be better detected.

Description

Technical Field

[0001] The invention belongs to the technical field of isotope labeling equipment, and in particular relates to a device and method for labeling carbon and nitrogen isotopes of plants. Background Art

[0002] Nitrogen is one of the key nutrients necessary for plant growth, especially in nitrogen-limited environments. Plant carbon transfer is one of the important processes that regulate the size of the soil carbon pool, and the intensity and direction of carbon and nitrogen transfer between plant organs will change with changes in the source-sink gradient. Although many scholars have conducted extensive research on carbon or nitrogen transfer between organs of different plants, people still have limited understanding of the interaction between carbon and nitrogen transfer processes between plant organs. Therefore, in-depth research on carbon and nitrogen transfer between plant organs and their interrelationships is of great significance for revealing the resource allocation strategy of plants and improving the global carbon and nitrogen cycle model.

[0003] Current technology adoption 15 The N isotope tracer method can effectively distinguish between exogenous nitrogen and endogenous nitrogen in plants. This method has become an efficient means to study nitrogen transfer between plants and soil, and between plant organs due to its advantages such as fast detection speed, accurate results and no interference with the ecosystem. 13 C labeling or enrichment technology has been successfully used to track the direction and intensity of carbon transfer between plant organs. The carbon cycle in plants is highly coupled to the nitrogen cycle, in which the energy generated by the carbon cycle supports the nitrogen uptake and assimilation process.

[0004] However, the prior art lacks a device for simultaneously tracking the carbon and nitrogen transfer of plants. Based on this, a device and method for plant carbon and nitrogen isotope labeling are proposed. Summary of the invention

[0005] The technical problem to be solved by the present invention is to provide a device and method for plant carbon and nitrogen isotope labeling in view of the deficiencies of the above-mentioned prior art, so as to solve the problems raised in the above-mentioned background technology.

[0006] In order to solve the above technical problems, the technical solution adopted by the present invention is:

[0007] In a first aspect, a device for labeling carbon and nitrogen isotopes of plants comprises a cell body, a double-layer support, an outer cover shell, a labeling tube, a fan, an air pump and a recorder;

[0008] The pool body is a circular pool body with an opening at the top, and the double-layer bracket is arranged in the middle of the pool body, a recorder is placed in the middle of the double-layer bracket, a plant platform is placed on the top of the double-layer bracket, plants are planted in the plant platform, and a marking device is also arranged on the plant platform. 15A marking tube of N, the marking tube being connected to the top of any plant;

[0009] The outer cover shell is a transparent cover shell with an opening at the bottom. The opening of the outer cover shell is arranged in the pool body, and the double-layer bracket, the plant platform and the plants are surrounded by the outer cover shell. A fan is arranged in the middle of the top of the outer cover shell, and an air pump is also arranged on one side of the outer cover shell. The fan and the air pump are respectively connected to the inner cavity of the outer cover shell. A syringe is also connected to the side wall of the outer cover shell through a silicone hose for later injection of the marked 13 CO 2 gas.

[0010] As a further illustration of the present invention, the recorder is used to record the air temperature, air humidity and carbon dioxide concentration in the outer housing.

[0011] As a further illustration of the present invention, a gas washing bottle is connected between the vacuum pump and the outer cover shell via a silicone hose.

[0012] As a further illustration of the present invention, the outer cover shell is made of transparent organic glass or transparent plastic with a thickness of 8 mm.

[0013] In a second aspect, a method for a device for plant carbon and nitrogen isotope labeling comprises the following steps:

[0014] S1. 15 When marking leaves with N isotopes, roll the leaf tips of the plants inward and place them in a marking tube, seal it, and fix the marking tube with a bracket.

[0015] S2, when 15 After the N isotope labeling is completed, remove the labeling tube and start the plant 13 C isotope labeling, first inject water into the pool, the water depth does not exceed the lowest platform of the double-layer support, place the plant platform and recorder on the double-layer support, and surround it with an outer cover to complete 13 C marks the chamber seal;

[0016] S3, then turn on the fan and the vacuum pump, 13 The gas in the C marked room is sent back to the 13 C labeling chamber, circulate the air for 30 minutes, and complete the labeling as described before 13 C labeling indoor gas dilution process, starving plants and then labeling to increase CO 2 Absorption and assimilation rate;

[0017] S4. Extract gas from the sample bag using a syringe 13 CO 2 Gas, injected into the 13In the C marking room, control the amount of gas injected, 60ml per plant per day 13 CO 2 , 99atom% 13 C, continuous labeling for 3 days, 5 hours per day, the plants complete the process through photosynthesis 13 C isotope gas marker, 13 CO 2 The labeled amount of gas can also be adjusted according to the specific needs of different plants.

[0018] Compared with the prior art, the present invention has the following advantages:

[0019] 1. The present invention 15 The N isotope leaf labeling method is simple and easy to use, and is different from the existing 15 Compared with the N isotope soil labeling method, leaf nitrogen absorption will not be diluted by the matrix during the labeling process, and the plant 15 The N mark works better.

[0020] 2. The present invention 13 The C isotope gas labeling lasted for a longer period of time, 3 consecutive days, 5 hours per day, and the 13 C marks the CO in the room 2 The gas was diluted for 30 minutes, starving the plants for a period of time before labeling, which increased the CO 2 Absorption and assimilation rate.

[0021] 3. The present invention can simultaneously track the direction and amplitude of carbon and nitrogen transfer between various plant organs in the short term. Compared with the existing technology, it takes the two elements C and N into more comprehensive consideration, and can better explore the amount, direction and coupling relationship of carbon and nitrogen transfer between various plant organs. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 It is a schematic diagram of the overall structure of the present invention.

[0023] Description of reference numerals:

[0024] 1-Pool body; 2-Double-layer bracket; 3-Outer cover shell; 4-Plant platform; 5-Plant; 6-Marking tube; 7-Fan; 8-Vacuum pump; 9-Recorder. DETAILED DESCRIPTION

[0025] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0026] like Figure 1 As shown, the present invention provides a technical solution: a device for labeling carbon and nitrogen isotopes of plants, comprising a cell body 1, a double-layer bracket 2, an outer cover shell 3, a labeling tube 6, a fan 7, an air pump 8 and a recorder 9;

[0027] The pool body 1 is a circular pool body with an open top, and the double-layer bracket 2 is arranged in the middle of the pool body 1. A recorder 9 is placed in the middle of the double-layer bracket 2. The recorder 9 is used to record the air temperature, air humidity and carbon dioxide concentration in the outer cover shell 3.

[0028] A plant platform 4 is placed on the top of the double-layer support 2. Plants 5 are planted in the plant platform 4. The plant platform 4 is also provided with a marking device. 15 A marking tube 6 of N, wherein the marking tube 6 is connected to the top of any plant 5;

[0029] The outer cover shell 3 is a transparent cover shell with an opening at the bottom, and the outer cover shell 3 is made of transparent organic glass with a thickness of 8 mm.

[0030] The outer cover shell 3 is opened and arranged in the pool body 1, and the double-layer bracket 2, the plant platform 4 and the plant 5 are surrounded by the outer cover shell 3. A fan 7 is arranged in the middle of the outer top of the outer cover shell 3, and an air pump 8 is also arranged on one side of the outer cover shell 3. The fan 7 and the air pump 8 are respectively connected to the inner cavity of the outer cover shell 3. The side wall of the outer cover shell 3 is also connected to a syringe through a silicone hose for injecting the marker later. 13 CO 2 gas.

[0031] A gas washing bottle is connected between the vacuum pump 8 and the outer housing 3 through a silicone hose, and a 1M NaOH solution is placed in the gas washing bottle.

[0032] The above-mentioned method for the device for plant carbon and nitrogen isotope labeling comprises the following steps:

[0033] S1. Perform the following steps on the leaves of plant 5: 15 When marking leaves with N isotope, the leaf tips of the leaves of the plant 5 are rolled inward and then placed in the marking tube 6 for sealing, and the marking tube 6 is fixed with a bracket;

[0034] S2, when 15 After the N isotope labeling is completed, remove the labeling tube and start the plant 13 C isotope labeling, first inject water into the pool body 1, the water depth does not exceed the lowest platform of the double-layer support 2, place the plant platform 4 and the recorder 9 on the double-layer support 2, and surround it with the outer cover 3 to complete 13 C marks the chamber seal;

[0035] S3, then turn on the fan 7 and the vacuum pump 8,13 The gas in the C marked room is sent back to the 13 C labeling chamber, circulate the air for 30 minutes, and complete the labeling as described before 13 C labeling indoor gas dilution process, starving plants for 5 seconds before labeling to increase CO 2 Absorption and assimilation rate;

[0036] S4. Extract gas from the sample bag using a syringe 13 CO 2 Gas, injected into the 13 In the C marking room, control the amount of gas injected, 60ml per plant per day 13 CO 2 , 99atom% 13 C, continuous labeling for 3 days, 5 hours per day, the plants complete the process through photosynthesis 13 C isotope gas marker, 13 CO 2 The labeled amount of gas can also be adjusted according to the specific needs of different plants.

[0037] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0038] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A device for labeling carbon and nitrogen isotopes in plants, characterized in that: It comprises a pool body (1), a double-layer bracket (2), an outer cover shell (3), a marking tube (6), a fan (7), an air pump (8) and a recorder (9); The pool body (1) is a circular pool body with an opening at the top, and the double-layer bracket (2) is arranged in the middle of the pool body (1), a recorder (9) is placed in the middle of the double-layer bracket (2), a plant platform (4) is placed on the top of the double-layer bracket (2), plants (5) are planted in the plant platform (4), and a device for marking is also provided on the plant platform (4). 15 A marking tube (6) of N, wherein the marking tube (6) is connected to the top of any plant (5); The outer cover shell (3) is a transparent cover shell with an opening at the bottom. The opening of the outer cover shell (3) is arranged in the pond body (1), and the double-layer support (2), the plant platform (4) and the plant (5) are surrounded by the outer cover shell (3). A fan (7) is arranged in the middle of the top of the outer cover shell (3). An air pump (8) is also arranged on one side of the outer cover shell (3). The fan (7) and the air pump (8) are respectively connected to the inner cavity of the outer cover shell (3). A syringe is also connected to the side wall of the outer cover shell (3) through a silicone hose for injecting the marked 13 CO2 gas.

2. The device for plant carbon and nitrogen isotope labeling according to claim 1, characterized in that: The recorder (9) is used to record the air temperature, air humidity and carbon dioxide concentration in the outer casing (3).

3. The device for plant carbon and nitrogen isotope labeling according to claim 1, characterized in that: A gas washing bottle is also connected between the vacuum pump (8) and the outer housing (3) via a silicone hose.

4. The device for plant carbon and nitrogen isotope labeling according to claim 1, characterized in that: The outer housing shell (3) is made of transparent organic glass or transparent plastic with a thickness of 8 mm.

5. A method for a plant carbon and nitrogen isotope labeling device according to any one of claims 1 to 4, characterized in that: The following steps are involved: S1. The leaves of the plant (5) are subjected to 15 When marking leaves with N isotopes, the leaf tips of the leaves of the plant (5) are rolled inward and then placed in a marking tube (6) and sealed, and the marking tube (6) is fixed with a bracket; S2, when 15 After N isotope labeling is completed, remove the labeling tube (6) and start the plant 13 C isotope labeling, firstly, water is injected into the pool body (1), the water depth does not exceed the lowest platform of the double-layer support (2), and the plant platform (4) and the recorder (9) are placed on the double-layer support (2), and the outer cover (3) is used to surround the plant platform (4) and the recorder (9) is placed on the double-layer support (2). 13 C marks the chamber seal; S3, then turn on the fan (7) and the vacuum pump (8), 13 The gas in the C marked room is sent back to the 13 C labeling chamber, circulate the air for 30 minutes, and complete the labeling as described before 13 C marks the process of dilution of indoor gas, which makes the plants (5) starve before marking to increase the absorption and assimilation rate of CO2; S4. Extract gas from the sample bag using a syringe 13 CO2 gas is injected into the 13 In the C marking room, control the amount of gas injected, 60ml per plant per day 13 CO2, 99atom% 13 C, continuous labeling for 3 days, 5 hours per day, the plants complete the process through photosynthesis 13 C isotope gas labeling.

6. A method for a plant carbon and nitrogen isotope labeling device according to claim 5, characterized in that: The labeled tube (6) contains 1.2 ml of 0.5% 15 N-urea, 99.7atom%, 6mg per leaf tip 15 N-urea solution, 99.7atom%, to be 15 The N-urea solution is completely absorbed by the leaves. 15 N mark.

7. A method for a plant carbon and nitrogen isotope labeling device according to claim 5, characterized in that: 1M NaOH solution is placed in the gas washing bottle.

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