Novel simple and convenient thrip breeding method

Abstract

The invention relates to the technical field of insect breeding, in particular to a novel simple and convenient thrip breeding method which comprises the following steps: S1, preliminary preparation of materials; s2, preparation and treatment of a culture medium; s3, leaf disinfection treatment; s4, placing and culturing the thrips; according to the novel simple and convenient thrip breeding method, existing breeding operation complexity is reduced, breeding cost is reduced, thrip breeding and follow-up inoculation work are convenient to observe, quantitative materials are accurately weighed, the growth and development conditions of the thrips are improved, the development speed and the breeding rate of the thrips are increased, the breeding process is simplified through the standardized operation process, and the breeding efficiency is improved. Compared with the prior art, the method has the advantages that labor cost is reduced, uncertainty caused by operation complexity is reduced, sterile operation is emphasized in each operation link, the risk of diseases and insect pests in the thrip breeding process is reduced, and the problem that diseases and insect pests are easily caused due to misoperation in an existing breeding method is solved.

Description

Technical Field

[0001] The present invention relates to the technical field of insect breeding, and particularly to a simple new method for breeding thrips. Background Technique

[0002] Thrips are small insects of the order Thysanoptera that mainly feed on the sap of plants. They use rasping-sucking mouthparts to pierce the plant epidermis and suck the sap inside the cells, resulting in faded plant petals, wrinkled leaves, and even the withering of the whole plant, which hinders plant growth and reduces yields. Although thrips are tiny in size, they have unique value in both scientific research and agriculture. In scientific research, they are often used as model organisms to study the physiological characteristics and genetic laws of insects. In agriculture, as pests, the research on their breeding technology helps to deeply understand their living habits, so as to formulate effective control strategies. At the same time, some predatory thrips also have potential applications in biological control. However, there are many drawbacks in the existing thrips breeding methods. It is difficult to regulate the breeding environment, and it is difficult to accurately control conditions such as temperature, humidity, and light, which often affect the growth and reproduction of thrips. In terms of feed, the existing formulations cannot meet the nutritional requirements of thrips, resulting in poor development and low reproduction rates. Moreover, the existing breeding operations are cumbersome, with high labor costs, and are also prone to diseases and pests due to improper operations. These problems seriously limit the development of thrips breeding. Summary of the Invention

[0003] The purpose of the present invention is to provide a simple new method for breeding thrips to solve the problems raised in the above background technique.

[0004] To achieve the above purpose, the present invention provides the following technical solution: A simple new method for breeding thrips, including the following steps:

[0005] S1. Preliminary preparation of materials;

[0006] S2. Preparation and treatment of the culture medium;

[0007] S3. Disinfection treatment of leaves;

[0008] S4. Placement and cultivation of thrips.

[0009] Preferably, the specific method in the step of S1. Preliminary preparation of materials is as follows:

[0010] S1-1. Accurately weigh 4.43 g of MS powder and 30 g of sucrose for standby;

[0011] S1-2. Measure 2,4-D with a concentration of 4 mg / L and 6-BA with a concentration of 0.22 mg / L for standby.

[0012] Preferably, the specific method in the step of S2. Preparation and treatment of the culture medium is as follows:

[0013] S2-1. Put the weighed MS powder and sucrose into a container, add an appropriate amount of water, stir to initially dissolve the MS powder and sucrose, then add the prepared 2,4-D solution and 6-BA solution, and continue to stir to ensure that all components are fully dissolved and evenly mixed;

[0014] S2-2. Use a suitable pH regulator to adjust the pH value of the mixed solution to 5.8;

[0015] S2-3. Add water to the solution to make the volume of the solution fixed at 1 L;

[0016] S2-4. Transfer the prepared culture medium to a suitable container, put it into a high-pressure steam sterilizer, and sterilize it at a temperature of 121 °C for 15 min;

[0017] S2-5. After sterilization, take out the culture medium and place it in an environment with a temperature of 25 °C for subsequent use.

[0018] Preferably, the specific method in the step S3 of leaf disinfection treatment is as follows:

[0019] S3-1. Select appropriate leaves and place them in a 50-ml centrifuge tube, with 5-10 trifoliate leaves in each tube;

[0020] S3-2. Add a sodium hypochlorite solution with 6% available chlorine containing 0.1% Tween 20 to the centrifuge tube containing the leaves, gently shake the centrifuge tube, and gently stir for 15 min to ensure that the leaf surface is fully in contact with the disinfection solution;

[0021] S3-3. Rinse the leaves with sterile water for 5 times, and ensure that the residual disinfectant is rinsed clean each time;

[0022] S3-4. After rinsing, take out the leaves from the centrifuge tube and place them on sterile filter paper to let the filter paper absorb the water on the leaf surface;

[0023] S3-5. In a laminar flow hood, transfer the water-absorbed leaves to the previously prepared culture medium.

[0024] Preferably, the specific method in the step S4 of thrips placement and culture treatment is as follows:

[0025] S4-1. Use a portable small insect trap to suck thrips into a 1-ml sterile and enzyme-free centrifuge tube;

[0026] S4-2. Quickly in a laminar flow hood, gently flick the centrifuge tube to make the thrips in the centrifuge tube flick into the culture medium with the leaves already placed;

[0027] S4-3: After placing the thrips, seal the culture medium with medical tape. Subsequently, place the culture medium in an incubator, set the culture temperature at 25°C, and conduct the culture under a light cycle of 16 / 8 h.

[0028] Preferably, in step S2-2, the pH adjustment process should be carried out under aseptic conditions. When adjusting the pH value, the pH regulator should be added dropwise slowly.

[0029] Preferably, in step S3-1, the trifoliate leaves can be selected from soybeans, kidney beans, alfalfa, etc. The collection time of the leaves should be selected during the vigorous growth period of the plant. Before collecting the leaves, the plant should be appropriately pruned to remove diseased leaves, yellow leaves, etc.

[0030] Preferably, in step S4-2, after the thrips are flicked onto the culture medium, immediately check the distribution of the thrips. If there is an aggregation phenomenon, appropriately adjust the distribution of the thrips.

[0031] Preferably, in step S4-3, the incubator has the functions of automatic control and monitoring of temperature, humidity, light, etc. The monitoring function should be able to display the environmental parameters in the incubator in real time and have the functions of data storage and export.

[0032] Preferably, in step S4-3, the medical tape has good sealing and air permeability. Before using the medical tape, check the quality of the tape. When pasting the medical tape, ensure that the tape fits tightly with the culture medium container without bubbles and gaps.

[0033] Compared with the prior art, the beneficial effects of the present invention are:

[0034] 1. The new method for breeding thrips provided by the present invention reduces the cumbersome existing breeding operations, lowers the breeding cost, and facilitates the observation of thrips reproduction and subsequent inoculation work;

[0035] 2. Accurately weighing a specific amount of MS powder and sucrose, as well as accurately measuring specific concentrations of plant growth regulators such as 2,4-D and 6-BA, helps to ensure the stability of the nutrient components of the culture medium, provides a relatively stable nutrient source for thrips, meets their growth and development needs, ensures that thrips can obtain sufficient nutrients at different growth stages, meets their physiological needs, thereby improving the growth and development status of thrips and increasing their development speed and reproduction rate;

[0036] 3. The steps of the new thrips breeding method are clear and definite. From the preliminary preparation of materials, the preparation and treatment of the culture medium, the disinfection treatment of leaves to the placement and cultivation of thrips, each link has a detailed standardized operation process. For example, in the link of leaf disinfection treatment, the proportion of the disinfectant solution, the treatment time and the number of rinses are clearly specified. Such a standardized operation process simplifies the breeding process, reduces labor costs and reduces the uncertainty caused by the complexity of operations.

[0037] 4. In each operation link, attention is paid to aseptic operation. For example, high-pressure steam sterilization is used in the sterilization process of the culture medium, and the leaves are disinfected with a sodium hypochlorite solution containing 0.1% Tween 20 and 6% available chlorine and rinsed multiple times, and key thrips transfer and placement operations are carried out in a laminar flow hood. These aseptic operation measures effectively prevent the introduction of external germs and pests, reduce the risk of diseases and pests occurring in the thrips breeding process, and overcome the problem that diseases and pests are easily caused by improper operations in the existing breeding methods. BRIEF DESCRIPTION OF THE DRAWINGS

[0038] Figure 1 It is a schematic diagram of alfalfa leaf treatment in the present invention;

[0039] Figure 2 It is a schematic diagram of thrips breeding for 15 days in the present invention;

[0040] Figure 3 It is a schematic diagram of thrips breeding for 30 days in the present invention. DETAILED DESCRIPTION OF THE INVENTION

[0041] Unless otherwise clearly specified in the context, nouns without quantifiers and nouns modified by "the" include singular and plural referents.

[0042] As used herein, the terms "comprising", "including", "having", "may", "containing" and their variants, as used in the specification and claims, are open transitional phrases, terms or words that require the presence of the specified component / step and allow the presence of other components / steps. However, such description should also be construed as describing the composition or method as "consisting of" and "consisting essentially of" the listed components / steps, which allows only the presence of the specified component / step and any inevitable impurities that may result therefrom, and excludes other components / steps.

[0043] The numerical values in the specification and claims of the present application should be understood to include the same numerical values when reduced to the same number of significant digits and numerical values that differ from the said values by less than the experimental error of the conventional measurement techniques used to determine the said values of the type described in the present application.

[0044] All ranges disclosed herein include the recited endpoints and are combinable independently (e.g., the range of "2 grams to 10 grams" includes the endpoints 2 grams and 10 grams, as well as all intermediate values).

[0045] The terms "about" and "approximately" can be used to include any numerical value that can vary without changing the basic function of that value. When used in connection with a range, "about" and "approximately" also disclose the range defined by the absolute values of the two endpoints. For example, "about 2 to about 4" also discloses the range of "2 to 4". Generally, the terms "about" and "approximately" can refer to ±10% of the indicated number. However, for temperature, the term "about" means ±1 °C.

[0046] Unless otherwise expressly specified, percentages of elements shall be considered to be weight percentages of the alloy.

[0047] This disclosure may relate to the temperature of certain method steps. It should be noted that these indicators generally refer to the temperature set by the heat source (such as a furnace), and not necessarily the temperature that the material being heated must reach.

[0048] The present invention provides a simple new method for breeding thrips, comprising the following steps:

[0049] S1. Preliminary preparation of materials;

[0050] S1-1. Accurately weigh 4.43 g of MS powder and 30 g of sucrose for standby;

[0051] S1-2. Measure 2,4-D with a concentration of 4 mg / L and 6-BA with a concentration of 0.22 mg / L for standby.

[0052] S2. Preparation and treatment of the culture medium;

[0053] S2-1. Put the weighed MS powder and sucrose into a container, add an appropriate amount of water, stir to dissolve the MS powder and sucrose preliminarily, and then add the prepared 2,4-D solution and 6-BA solution, and continue to stir to ensure that all components are fully dissolved and homogenized;

[0054] S2-2. Use a suitable pH regulator to adjust the pH value of the mixed solution to 5.8. The pH adjustment process should be carried out under sterile conditions. When adjusting the pH value, the pH regulator should be added dropwise slowly;

[0055] S2-3. Add water to the solution to make the volume of the solution fixed at 1 L;

[0056] S2-4. Transfer the prepared culture medium to a suitable container, put it into a high-pressure steam sterilizer, and sterilize it at a temperature of 121 °C for 15 min;

[0057] S2-5. After sterilization is completed, take out the culture medium and place it in an environment with a temperature of 25°C for subsequent use.

[0058] S3. Leaf disinfection treatment;

[0059] S3-1. Select appropriate leaves and place them in a 50 ml centrifuge tube. Put 5 - 10 trifoliate leaves in each tube. The trifoliate leaves can be selected from soybeans, kidney beans, alfalfa, etc. The collection time of the leaves should be selected during the vigorous growth period of the plant. Before collecting the leaves, appropriately prune the plant to remove diseased leaves, yellow leaves, etc.

[0060] S3-2. Add a sodium hypochlorite solution with 6% available chlorine containing 0.1% Tween 20 to the centrifuge tube containing the leaves. Gently shake the centrifuge tube and gently stir for 15 min to ensure that the leaf surface is fully in contact with the disinfection solution.

[0061] S3-3. Rinse the leaves with sterile water 5 times, and ensure that the residual disinfectant is rinsed clean each time.

[0062] S3-4. After rinsing is completed, take out the leaves from the centrifuge tube and place them on sterile filter paper to let the filter paper absorb the water on the leaf surface.

[0063] S3-5. In a laminar flow hood, transfer the leaves with absorbed water to the previously prepared culture medium.

[0064] S4. Thrips placement and cultivation;

[0065] S4-1. Use a portable small insect trap to suck thrips into a 1 ml sterile and enzyme-free centrifuge tube.

[0066] S4-2. Quickly in a laminar flow hood, gently flick the centrifuge tube to make the thrips in the centrifuge tube bounce onto the culture medium with the leaves already placed on it. After the thrips bounce onto the culture medium, immediately check the distribution of the thrips. If there is an aggregation phenomenon, appropriately adjust the distribution of the thrips.

[0067] S4-3. After placing the thrips is completed, seal the culture medium with medical tape. Subsequently, place the culture medium in an incubator, set the culture temperature to 25°C, and the light cycle to 16 / 8 h for cultivation. The incubator has automatic control and monitoring functions for conditions such as temperature, humidity, and light. The monitoring function should be able to display the environmental parameters in the incubator in real time and have data storage and export functions. The medical tape has good sealing and air permeability. Before using the medical tape, check the quality of the tape. When sticking the medical tape, ensure that the tape fits tightly with the culture medium container without air bubbles and gaps.

[0068] Specific steps: Accurately weigh 4.43 g of MS powder and 30 g of sucrose for standby, measure 2,4-D with a concentration of 4 mg / L and 6-BA with a concentration of 0.22 mg / L for standby. Put the weighed MS powder and sucrose into a container, add an appropriate amount of water, and stir to preliminarily dissolve the MS powder and sucrose. Then, add the prepared 2,4-D solution and 6-BA solution, and continue to stir to ensure that all components are fully dissolved and evenly mixed. Use a suitable pH regulator to adjust the pH value of the mixed solution to 5.8. The pH adjustment process should be carried out under sterile conditions. When adjusting the pH value, the pH regulator should be slowly added dropwise, and water should be added to the solution to make the volume of the solution fixed at 1 L. Transfer the prepared medium to a suitable container, put it into a high-pressure steam sterilizer, and sterilize it at a temperature of 121 °C for 15 min. After sterilization, take out the medium and place it in an environment with a temperature of 25 °C for subsequent use. Select appropriate leaves and place them in a 50 ml centrifuge tube, with 5-10 trifoliate leaves placed in each tube. The trifoliate leaves can be selected from soybeans, kidney beans, alfalfa, etc., and the collection time of the leaves should be selected during the vigorous growth period of the plant. Before collecting the leaves, appropriately prune the plant to remove diseased leaves, yellow leaves, etc. Add a sodium hypochlorite solution with an available chlorine of 6% containing one-thousandth of Tween 20 to the centrifuge tube containing the leaves, gently shake the centrifuge tube, and gently stir for 15 min to ensure that the leaf surface is fully in contact with the disinfection solution. Rinse the leaves with sterile water 5 times, and ensure that the residual disinfectant is rinsed off each time. After rinsing, take out the leaves from the centrifuge tube and place them on sterile filter paper to let the filter paper absorb the water on the leaf surface. In a laminar flow hood, transfer the dried leaves to the previously prepared medium. Use a portable small insect trap to suck thrips into a 1 ml sterile and enzyme-free centrifuge tube, and quickly in the laminar flow hood, gently flick the centrifuge tube to make the thrips in the centrifuge tube flick onto the medium where the leaves have been placed. After the thrips are flicked onto the medium, immediately check the distribution of the thrips. If there is an aggregation phenomenon, appropriately adjust the distribution of the thrips. After the thrips are placed, seal the medium with medical tape. Subsequently, place the medium in an incubator, set the culture temperature to 25 °C, and culture it under a light cycle of 16 / 8 h. The incubator has automatic control and monitoring functions for conditions such as temperature, humidity, and light. The monitoring function should be able to display the environmental parameters in the incubator in real time and have data storage and export functions. The medical tape has good sealing and air permeability. Before using the medical tape, check the quality of the tape. When sticking the medical tape, ensure that the tape fits tightly with the medium container without bubbles and gaps.

[0069] Example 1:

[0070] S1. Preliminary preparation of materials;

[0071] S1-1. Weigh 4.43 g of MS powder and 30 g of sucrose accurately for later use;

[0072] S1-2. Measure 2,4-D with a concentration of 4 mg / L and 6-BA with a concentration of 0.22 mg / L for later use.

[0073] S2. Preparation and treatment of the culture medium;

[0074] S2-1. Put the weighed MS powder and sucrose into a container, add an appropriate amount of water, stir to dissolve the MS powder and sucrose preliminarily, then add the prepared 2,4-D solution and 6-BA solution, and continue to stir to ensure that all components are fully dissolved and evenly mixed;

[0075] S2-2. Use a suitable pH regulator to adjust the pH value of the mixed solution to 5.8. The pH adjustment process should be carried out under sterile conditions. When adjusting the pH value, the pH regulator should be added drop by drop slowly;

[0076] S2-3. Add water to the solution to make the volume of the solution fixed at 1 L;

[0077] S2-4. Transfer the prepared culture medium to a suitable container, put it into a high-pressure steam sterilizer, and sterilize it at a temperature of 121 °C for 15 min;

[0078] S2-5. After sterilization, take out the culture medium and place it in an environment with a temperature of 25 °C for subsequent use.

[0079] S3. Disinfection treatment of leaves;

[0080] S3-1. Select fresh and healthy alfalfa leaves, cut the alfalfa leaves into pieces with a size of 3 cm 2 and place them in a 50-ml centrifuge tube, with 7 alfalfa leaves placed in each tube;

[0081] S3-2. Add sodium hypochlorite solution with 6% available chlorine containing 0.1% Tween 20 to the centrifuge tube containing alfalfa leaves, gently shake the centrifuge tube, and gently stir for 15 min to ensure that the surface of the alfalfa leaves is fully in contact with the disinfection solution;

[0082] S3-3. Rinse the alfalfa leaves with sterile water for 5 times, and ensure that the residual disinfectant is rinsed clean each time;

[0083] S3-4. After rinsing, take out the alfalfa leaves from the centrifuge tube and place them on sterile filter paper to let the filter paper absorb the water on the surface of the alfalfa leaves;

[0084] S3-5. Transfer the alfalfa leaves with the water absorbed in a clean bench to the previously prepared culture medium.

[0085] S4, Thrips Placement and Cultivation;

[0086] S4-1. Use a portable small insect trap to suck thrips into a 1-ml sterile and enzyme-free centrifuge tube.

[0087] S4-2. Quickly, within a laminar flow hood, gently flick the centrifuge tube to make the thrips in the centrifuge tube bounce onto the culture medium with alfalfa leaves already placed. After the thrips are bounced onto the culture medium, immediately check the distribution of the thrips. If there is an aggregation phenomenon, appropriately adjust the distribution of the thrips.

[0088] S4-3. After the thrips placement is completed, seal the culture medium with medical tape. Subsequently, place the culture medium in an incubator, set the culture temperature at 25 °C, and perform the culture with a light cycle of 16 / 8 h.

[0089] Specific steps: Accurately weigh 4.43 g of MS powder and 30 g of sucrose for standby. Measure 2,4-D with a concentration of 4 mg / L and 6-BA with a concentration of 0.22 mg / L for standby. Put the weighed MS powder and sucrose into a container, add an appropriate amount of water, stir to make the MS powder and sucrose preliminarily dissolve. Then, add the prepared 2,4-D solution and 6-BA solution, and continue to stir to ensure that all components are fully dissolved and evenly mixed. Use a suitable pH regulator to adjust the pH value of the mixed solution to 5.8. The pH adjustment process should be carried out under sterile conditions. When adjusting the pH value, slowly drip the pH regulator and add water to the solution to make the volume of the solution fixed at 1 L. Transfer the prepared culture medium to a suitable container, put it into a high-pressure steam sterilizer, sterilize at a temperature of 121 °C for 15 min. After sterilization is completed, take out the culture medium and place it in an environment with a temperature of 25 °C for subsequent use. Select fresh and healthy alfalfa leaves and cut the alfalfa leaves into 3 cm 2Size, place it in a 50 ml centrifuge tube, put 7 alfalfa leaves in each tube. Add sodium hypochlorite solution with 6% available chlorine containing 0.1% Tween 20 to the centrifuge tube with alfalfa leaves. Gently shake the centrifuge tube and gently stir for 15 min to ensure that the surface of the alfalfa leaves is fully in contact with the disinfection solution. Rinse the alfalfa leaves with sterile water for 5 times, and ensure that the residual disinfectant is rinsed off each time. After rinsing, take out the alfalfa leaves from the centrifuge tube and place them on sterile filter paper to let the filter paper absorb the water on the surface of the alfalfa leaves. In the ultra-clean workbench, transfer the water-absorbed alfalfa leaves to the previously prepared medium. Use a portable small insect trap to suck thrips into a 1 ml sterile and enzyme-free centrifuge tube. Quickly, in the ultra-clean workbench, gently flick the centrifuge tube to make the thrips in the centrifuge tube bounce onto the medium with alfalfa leaves already placed. After the thrips are bounced onto the medium, immediately check the distribution of the thrips. If there is an aggregation phenomenon, appropriately adjust the distribution of the thrips. After placing the thrips, seal the medium with medical tape. Subsequently, place the medium in an incubator, set the culture temperature at 25 °C, and culture with a light cycle of 16 / 8 h.

[0090] Example 2:

[0091] S1. Preliminary preparation of materials;

[0092] S1-1. Accurately weigh 4.43 g of MS powder and 30 g of sucrose for standby;

[0093] S1-2. Measure 2,4-D with a concentration of 4 mg / L and 6-BA with a concentration of 0.22 mg / L for standby.

[0094] S2. Preparation and treatment of the medium;

[0095] S2-1. Put the weighed MS powder and sucrose into a container, add an appropriate amount of water, stir to make the MS powder and sucrose dissolve preliminarily. Then, add the prepared 2,4-D solution and 6-BA solution, and continue to stir to ensure that all components are fully dissolved and evenly mixed;

[0096] S2-2. Use a suitable pH regulator to adjust the pH value of the mixed solution to 5.8. The pH adjustment process should be carried out under sterile conditions. When adjusting the pH value, the pH regulator should be slowly added dropwise;

[0097] S2-3. Add water to the solution to make the volume of the solution fixed at 1 L;

[0098] S2-4. Transfer the prepared medium to a suitable container, put it into a high-pressure steam sterilizer, and sterilize at a temperature of 121 °C for 15 min;

[0099] S2-5. After sterilization is completed, take out the culture medium and place it in an environment with a temperature of 25 °C for subsequent use.

[0100] S3. Leaf disinfection treatment;

[0101] S3-1. Select fresh and healthy kidney bean leaves, cut the kidney bean leaves into pieces of 2 cm 2 in size, place them in a 50 ml centrifuge tube, and place 10 kidney bean leaves in each tube;

[0102] S3-2. Add sodium hypochlorite solution with 6% available chlorine containing 0.1% Tween 20 to the centrifuge tube containing kidney bean leaves, gently shake the centrifuge tube, and gently stir for 15 min to ensure that the surface of the kidney bean leaves is fully in contact with the disinfection solution;

[0103] S3-3. Rinse the kidney bean leaves with sterile water for 5 times, and ensure that the residual disinfectant is rinsed off each time;

[0104] S3-4. After rinsing is completed, take out the kidney bean leaves from the centrifuge tube and place them on sterile filter paper to let the filter paper absorb the water on the surface of the kidney bean leaves;

[0105] S3-5. Transfer the kidney bean leaves with the water absorbed in the ultra-clean workbench to the previously prepared culture medium.

[0106] S4. Thrips placement and cultivation;

[0107] S4-1. Use a portable small insect trap to suck thrips into a 1 ml sterile and enzyme-free centrifuge tube;

[0108] S4-2. Quickly in the ultra-clean workbench, gently flick the centrifuge tube to make the thrips in the centrifuge tube flick onto the culture medium where the kidney bean leaves have been placed. After the thrips are flicked onto the culture medium, immediately check the distribution of the thrips. If there is an aggregation phenomenon, appropriately adjust the distribution of the thrips;

[0109] S4-3. After the thrips are placed, seal the culture medium with medical tape. Subsequently, place the culture medium in an incubator, set the culture temperature at 25 °C, and the light cycle at 16 / 8 h for cultivation.

[0110] Specific steps: Accurately weigh 4.43 g of MS powder and 30 g of sucrose for standby, measure 2,4-D with a concentration of 4 mg / L and 6-BA with a concentration of 0.22 mg / L for standby. Put the weighed MS powder and sucrose into a container, add an appropriate amount of water, and stir to initially dissolve the MS powder and sucrose. Then, add the prepared 2,4-D solution and 6-BA solution, and continue to stir to ensure that all components are fully dissolved and evenly mixed. Use a suitable pH regulator to adjust the pH value of the mixed solution to 5.8. The pH adjustment process should be carried out under sterile conditions. When adjusting the pH value, the pH regulator should be slowly added dropwise. Add water to the solution to make the volume of the solution fixed at 1 L. Transfer the prepared culture medium to a suitable container, put it into a high-pressure steam sterilizer, and sterilize it at a temperature of 121 °C for 15 min. After sterilization is completed, take out the culture medium and place it in an environment with a temperature of 25 °C for subsequent use. Select fresh and healthy kidney bean leaves, cut the kidney bean leaves into pieces of 2 cm 2 in size, place them in a 50 ml centrifuge tube, and place 10 kidney bean leaves in each tube. Add sodium hypochlorite solution with an available chlorine of 6% containing one-thousandth of Tween 20 to the centrifuge tube containing the kidney bean leaves, gently shake the centrifuge tube, and gently stir for 15 min to ensure that the surface of the kidney bean leaves is fully in contact with the disinfection solution. Rinse the kidney bean leaves with sterile water 5 times, and ensure that the residual disinfectant is rinsed off each time. After rinsing is completed, take out the kidney bean leaves from the centrifuge tube and place them on sterile filter paper to let the filter paper absorb the water on the surface of the kidney bean leaves. In a laminar flow hood, transfer the kidney bean leaves with the water absorbed to the previously prepared culture medium. Use a portable small insect trap to suck thrips into a 1 ml sterile and enzyme-free centrifuge tube. Quickly, in the laminar flow hood, gently flick the centrifuge tube to make the thrips in the centrifuge tube flick onto the culture medium where the kidney bean leaves have been placed. After the thrips are flicked onto the culture medium, immediately check the distribution of the thrips. If there is an aggregation phenomenon, appropriately adjust the distribution of the thrips. After the thrips are placed, seal the culture medium with medical tape. Subsequently, place the culture medium in an incubator, set the culture temperature to 25 °C, and perform the culture with a light cycle of 16 / 8 h.

[0111] Finally, it should be noted that the above are only the preferred embodiments of the present invention and are not used to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, for those skilled in the art, they can still modify the technical solutions recorded in the foregoing embodiments, or perform equivalent replacements for some of the technical features. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be included within the protection scope of the present invention.

Claims

1. A simple new method for breeding thrips, characterized in that: The following steps are involved: S1. Preliminary preparation of materials; S2, medium preparation and processing; S3, leaf disinfection; S4. Thrips placement and cultivation.

2. A simple new method for breeding thrips according to claim 1, characterized in that: The specific method in the preliminary preparation step of S1, materials is as follows: S1-1. Accurately weigh 4.43 g MS powder and 30 g sucrose for later use; S1-2. Measure 4 mg / L 2,4-D and 0.22 mg / L 6-BA for later use.

3. A simple new method for breeding thrips according to claim 1, characterized in that: The specific method in the step of S2, medium preparation and treatment is as follows: S2-1. Put the weighed MS powder and sucrose into a container, add an appropriate amount of water, and stir to dissolve the MS powder and sucrose preliminarily. Then, add the prepared 2,4-D solution and 6-BA solution, and continue stirring to ensure that all ingredients are fully dissolved evenly. S2-2, using a suitable pH adjuster, adjusting the pH value of the mixed solution to 5.8; S2-3, add water to the solution to make the volume of the solution to 1L; S2-4. Transfer the prepared culture medium to a suitable container, place it in a high pressure steam sterilizer, and sterilize it at 121°C for 15 minutes; S2-5. After sterilization, take out the culture medium and place it in an environment with a temperature of 25°C for subsequent use.

4. A simple new method for breeding thrips according to claim 1, characterized in that: The specific method in S3, the leaf disinfection treatment step is as follows: S3-1. Select appropriate leaves and place them in 50ml centrifuge tubes, with 5-10 trifoliate leaves placed in each tube; S3-2. Add 6% sodium hypochlorite solution containing 1 / 1000 Tween 20 and effective chlorine to the centrifuge tube containing the leaves. Gently shake the centrifuge tube and stir gently for 15 minutes to ensure that the surface of the leaves is fully exposed to the disinfectant solution. S3-3, rinse the leaves with sterile water for 5 times, making sure to rinse away any remaining disinfectant each time; S3-4. After rinsing, take the leaves out of the centrifuge tube and place them on sterile filter paper to allow the filter paper to absorb the moisture on the surface of the leaves; S3-5. In the clean bench, transfer the dried leaves to the previously prepared culture medium.

5. A simple new method for breeding thrips according to claim 1, characterized in that: The specific method in S4, thrips placement and cultivation step is as follows: S4-1. Use a portable small insect trap to suck the thrips into a 1 ml sterile enzyme-free centrifuge tube; S4-2. Quickly flick the centrifuge tube gently in the clean bench to make the thrips in the centrifuge tube bounce into the culture medium where the leaves have been placed; S4-3. After the thrips were placed, the culture medium was sealed with medical tape, and then placed in an incubator, the culture temperature was set to 25°C, and the light cycle was set to 16 / 8h for cultivation.

6. A simple new method for breeding thrips according to claim 3, characterized in that: In the step S2-2, the pH adjustment process should be carried out under sterile conditions, and the pH adjuster should be slowly added dropwise when adjusting the pH value.

7. A simple new method for breeding thrips according to claim 4, characterized in that: In the step S3-1, the trifoliate leaves may be selected from soybeans, beans, alfalfa, etc., and the leaves should be collected during the vigorous growth period of the plants. Before collecting the leaves, the plants should be properly pruned to remove diseased leaves, yellow leaves, etc.

8. A simple new method for breeding thrips according to claim 5, characterized in that: In the step S4-2, after the thrips are ejected onto the culture medium, the distribution of the thrips is immediately checked, and if there is aggregation, the distribution of the thrips is appropriately adjusted.

9. A simple new method for breeding thrips according to claim 5, characterized in that: In the step S4-3, the incubator has the function of automatic control and monitoring of temperature, humidity, light and other conditions. The monitoring function should be able to display various environmental parameters in the incubator in real time and have data storage and export functions.

10. A simple new method for breeding thrips according to claim 5, characterized in that: In the S4-3 step, the medical tape has good sealing and air permeability. Before using the medical tape, check the quality of the tape. When sticking the medical tape, ensure that the tape fits tightly to the culture medium container without bubbles and gaps.

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