Compound glutaraldehyde disinfectant for silkworms

Abstract

The invention discloses a compound glutaraldehyde disinfectant for silkworms. Belongs to the technical field of veterinary drugs (silkworm drugs). The invention provides a veterinary drug which is prepared from glutaraldehyde, didodecyl dimethyl ammonium chloride and trisodium phosphate according to the use concentration ratio of 2.5%: 0.2%: 0.3%. The defects that the disinfection effect on CPB, NPB, B.t spores and microparticles is poor when glutaraldehyde is singly used, the synergistic effect among prescriptions is obviously enhanced, the disinfection effect on microparticles is poor when a surfactant is singly used, and dependence, toxicity, corrosivity and pungent smell on chlorine-containing disinfection drugs are strong in the current sericulture production are overcome, and the harm to human bodies is greatly reduced.

Description

Technical Field

[0001] The invention belongs to the technical field of veterinary drugs (silkworm drugs), and is mainly used for disinfecting silkworm rooms, silkworm appliances and the environment in silkworm breeding, and relates to a compound glutaraldehyde disinfectant for silkworms; specifically, it relates to a compound glutaraldehyde disinfectant for silkworms and its usage method. Background Art

[0002] At present, most of the disinfectants for silkworm rooms and silkworm appliances used in sericulture production are chlorine-containing preparations such as trichloroisocyanuric acid powder, compound calcium hypochlorite powder, trichloroisocyanuric acid trisodium phosphate powder, etc. These preparations can kill the pathogens of silkworm viral diseases, fungal diseases, bacterial diseases, and protozoal diseases. However, their disadvantages are: strong corrosiveness and pungent smell, which have a greater impact on the human body.

[0003] At present, there are mainly two categories of common disinfectants for silkworm rooms and silkworm appliances in sericulture production: one is chlorine-containing preparations, mainly including bleaching powder (bleaching powder concentrate), compound calcium hypochlorite powder, trichloroisocyanuric acid powder, trichloroisocyanuric acid trisodium phosphate powder, etc.; the other is formaldehyde preparations, such as formalin lime slurry solution, sodium dichloroisocyanurate fumigant, etc.

[0004] Among them, although bleaching powder (bleaching powder concentrate), trichloroisocyanuric acid powder, and trichloroisocyanuric acid trisodium phosphate powder are broad-spectrum disinfectants, they are highly corrosive to metal products and cotton and linen fabrics. Trichloroisocyanuric acid powder has a strong pungent smell, and bleaching powder has poor stability and is difficult to store; with the improvement of living standards, the use range of the above-mentioned silkworm disinfectants will be greatly affected;

[0005] Compound calcium hypochlorite powder is a patented product, which has the advantages of comprehensive disinfection, low corrosiveness, and small pungent smell. However, the finished product has a short shelf life, the aqueous solution is turbid, it is inconvenient to use, and it takes a long time to be effective for leaf surface disinfection of mulberry leaves, affecting its use effect;

[0006] Although formalin lime slurry solution is a broad-spectrum disinfectant, it has a strong pungent smell. Operators need to wear gas masks, and the disinfection effect is often limited by temperature. When the temperature is lower than 24°C, the disinfection effect is significantly affected, so it is not widely used in rural sericulture areas;

[0007] Surfactant disinfectants such as Cankangning were used in sericulture production from the 1980s to the early 1990s. Its advantages are no pungent smell, no corrosion, and disinfection effect on fungal diseases, bacterial diseases and viral diseases. However, single use has no disinfection effect on microsporidia, and it is basically no longer used in sericulture production now. Summary of the Invention

[0008] In view of the above problems, the object of the present invention is to propose a compound glutaraldehyde disinfectant for silkworms.

[0009] The technical solution of the present invention is as follows: A compound glutaraldehyde disinfectant for silkworms of the present invention comprises glutaraldehyde, dodecyl dimethyl ammonium chloride and trisodium phosphate;

[0010] The concentration ratio of each component is: glutaraldehyde 2.5%, dodecyl dimethyl ammonium chloride 0.2%, and trisodium phosphate 0.3%;

[0011] The structural formula of glutaraldehyde is as follows:

[0012]

[0013] The operation steps of its usage method are as follows:

[0014] Step (1): Prepare a solution of glutaraldehyde and dodecyl dimethyl ammonium chloride in purified water according to the content requirements in proportion and fill it into bottle A;

[0015] Step (2): Take trisodium phosphate and fill it into bottle B;

[0016] Step (3): When in use, take the content of bottle A, dissolve it with a certain amount of water, add the content of bottle B and continue to dissolve, and then perform spray disinfection or immersion disinfection.

[0017] Further, in step (1), the amount of the prepared solution is: 500 ml.

[0018] Further, in step (3), when in use, take the content of bottle A, dissolve it with 4500 ml of water, add the content of bottle B and continue to dissolve.

[0019] Further, in step (3), the dissolved mixture is used for spray disinfection or immersion disinfection of the silkworm room, silkworm tools and the silkworm-raising environment.

[0020] Further, the mixture disinfects bacterial diseases, viral diseases, pebrine diseases and fungal diseases in the silkworm room, silkworm tools and the silkworm-raising environment.

[0021] Further, in step (3), the spraying amount is 225 ml per square meter.

[0022] Further, in step (3), keep it moist for 35 minutes after disinfection.

[0023] The beneficial effects of the present invention are as follows: The present invention provides a preparation composed of glutaraldehyde, didodecyldimethylammonium chloride, and trisodium phosphate in a usage concentration formulation ratio of 2.5%:0.2%:0.3%: 1. It has the characteristics of high efficiency, broad spectrum, and thorough disinfection; the killing rates of the pathogens of silkworm viral diseases, fungal diseases, bacterial diseases, and protozoal diseases all reach over 98%; 2. The synergistic effect between the prescriptions is obvious, overcoming the disadvantage of poor disinfection effect of surfactants on microparticles; 3. It solves the problems of poor disinfection effects of using glutaraldehyde alone on CPB, NPB, B.t spores, and microparticles, the obvious enhancement of the synergistic effect between prescriptions, and the poor disinfection effect of using surfactants alone on microparticles; 4. It completely overcomes the dependence on chlorine-containing disinfection drugs in current sericulture production, as well as the disadvantages of strong toxicity, corrosiveness, and irritating odor. Detailed Implementation Modes

[0024] The following further elaborates on the specific technical solutions of the present invention in combination with specific examples.

[0025] A compound glutaraldehyde disinfectant for silkworms according to the present invention, a compound disinfectant for silkworms used for disinfecting silkworm rooms, silkworm tools, and the environment, is composed of glutaraldehyde, didodecyldimethylammonium chloride, and trisodium phosphate. The concentration formulation ratio is: glutaraldehyde 2.5% + didodecyldimethylammonium chloride 0.2% + trisodium phosphate 0.3%; the specific specification is: glutaraldehyde 125 g + didodecyldimethylammonium chloride 10 g + trisodium phosphate 15 g; glutaraldehyde and didodecyldimethylammonium chloride are formulated into a solution for packaging, and trisodium phosphate is packaged alone. When in use, both are dissolved in water separately and then mixed together, and keep moist for 35 minutes.

[0026] The raw material of the compound disinfectant for silkworms in the present invention, glutaraldehyde, has the English name: Glutaraldehyde; the molecular formula is: C 5 H 8 O 2 ; the relative molecular weight is 100.12, and the structural formula is:

[0027]

[0028] The specification requirements for glutaraldehyde: the content ≥ 50%;

[0029] The content of didodecyldimethylammonium chloride ≥ 70%;

[0030] The content of trisodium phosphate ≥ 95%;

[0031] Glutaraldehyde, didodecyldimethylammonium chloride, and trisodium phosphate are formulated in a usage concentration formulation ratio of 2.5%:0.2%:0.3%.

[0032] The present invention is used for disinfecting silkworm rearing rooms, silkworm appliances and the environment; it has a good killing effect on the pathogens of silkworm virus diseases, fungal diseases, bacterial diseases and protozoal diseases; the main component glutaraldehyde is a highly effective disinfectant and sterilant, but the disinfection effect of using glutaraldehyde alone on CPB, NPB, B.t spores and microsporidia is poor; dodecyl dimethyl ammonium chloride is a surfactant disinfectant, which has a good disinfection effect on Aspergillus flavus spores, but has basically no disinfection effect on silkworm microsporidiosis, CPB, NPB and B.t spores when used alone; when used in combination with glutaraldehyde and trisodium phosphate, it improves the disinfection effect on Aspergillus flavus spores and strengthens the disinfection effect of glutaraldehyde on CPB, NPB, B.t spores and microsporidia; the compound disinfectant for silkworms used for disinfecting silkworm rearing rooms, silkworm appliances and the environment integrates the functions of each component of the prescription and has an obvious synergistic effect; it has basically no corrosion on carbon steel, stainless steel, copper and aluminum, and the toxicity, corrosivity and pungent smell are significantly reduced.

[0033] In the use of the present invention, take the content of bottle A (mixed solution of glutaraldehyde and dodecyl dimethyl ammonium chloride), dissolve it with 4500 ml of water, then add the content of bottle B (trisodium phosphate) and continue to dissolve, and then carry out spray disinfection or immersion disinfection; the spraying amount is about 225 ml per square meter, and keep it moist for 35 minutes; do not mix it with anionic surfactants and inorganic salt disinfectants during use, and use it within 40 minutes after the solution is prepared.

[0034] Example 1

[0035] Disinfection effect tests of various ratios of the compound glutaraldehyde disinfectant for silkworms, single-agent preparations, control drugs and blank controls. The disinfection effect is calculated based on the incidence rate and expressed as the inactivation rate.

[0036] (1) Disinfection effect of the disinfectant on NPB;

[0037] Table 1 Disinfection effect of the disinfectant on NPB

[0038]

[0039] (2) Disinfection effect of the disinfectant on CPB;

[0040] Table 2 Disinfection effect of the disinfectant on CPB

[0041]

[0042] (3) Disinfection effect of the disinfectant on microsporidia;

[0043] Table 3 Disinfection effect of the disinfectant on microsporidia

[0044]

[0045]

[0046] (4) Disinfection effect of disinfectants on Beauveria bassiana spores;

[0047] Table 4 Disinfection effect of disinfectants on Beauveria bassiana spores

[0048]

[0049] (5) Disinfection effect of disinfectants on Aspergillus flavus spores;

[0050] Table 5 Disinfection effect of disinfectants on Aspergillus flavus spores

[0051]

[0052] (6) Disinfection effect of disinfectants on B.t spores;

[0053] Table 6 Disinfection effect of disinfectants on B.t spores

[0054]

[0055] Note: D1221 represents dodecyl dimethyl ammonium chloride

[0056] The above tests show that the disinfection effects of glutaraldehyde alone on CPB, NPB, B.t spores, and microparticles are all inferior to those of compound disinfectants; the addition of adjuvants dodecyl dimethyl ammonium chloride and trisodium phosphate has an obvious synergistic effect, strengthening the disinfection effects of glutaraldehyde on CPB, NPB, B.t spores, and microparticles.

[0057] Dodecyl dimethyl ammonium chloride has a good disinfection effect on Aspergillus flavus spores, but has basically no disinfection effect on Nosema bombycis, CPB, NPB, and B.t spores when used alone; when mixed with glutaraldehyde and trisodium phosphate, it improves the disinfection effect on Aspergillus flavus spores, and the synergistic disinfection effect is obvious.

[0058] Taking the highest glutaraldehyde concentration and the longest action time in the recommended disinfection combinations for different pathogens as the recommended concentration ratio of the compound disinfectant; 2.5% glutaraldehyde + 0.2% dodecyl dimethyl ammonium chloride + 0.3% trisodium phosphate, disinfecting for 35 minutes is the best, which is the content of the present invention.

[0059] Example 2

[0060] The comparative test of the corrosion of carbon steel, aluminum, copper, and stainless steel between the present invention and commonly used chlorine preparation drugs in the market is expressed by the corrosion rate (R) / (mm / a).

[0061] Table 7 Results of metal corrosion test

[0062] Disinfectant solution Corrosion rate (R) / (mm / a) Compound glutaraldehyde disinfectant solution Copper 0.0039 Stainless steel 0.0000 Aluminum 0.0000 Carbon steel -0.0679 Yadijing 250-fold solution Copper 3.2589 Stainless steel 0.0016 Aluminum 1.1683 Carbon steel 4.4931

[0063] Table 8 Results of Metal Corrosion Test

[0064]

[0065]

[0066] It can be seen from the above tests that the present invention has basically no corrosion on carbon steel, stainless steel, copper, and aluminum; while Yadi Jingchu has basically no corrosion on stainless steel, but has severe corrosion on copper, aluminum, and carbon steel.

[0067] Example 3

[0068] The compound glutaraldehyde disinfectant for silkworms of the present invention is composed of glutaraldehyde, dodecyl dimethyl ammonium chloride, and trisodium phosphate. For actual use, take 125 g of glutaraldehyde and 10 g of dodecyl dimethyl ammonium chloride, and prepare a 500 ml solution with purified water according to the content requirements, then fill it into bottle A, and put 15 g of trisodium phosphate into bottle B. During use, take the content of bottle A, dissolve it in 4500 ml of water, then add the content of bottle B and continue to dissolve, and then spray or immerse the silkworm rearing room, silkworm rearing tools and the rearing environment for disinfection; the spraying amount is about 225 ml per square meter, and keep it moist for 35 minutes after disinfection; conduct a pilot test for large-scale production in the silkworm rearing area and use it in farmers' households and family farms respectively; use 1000 bags of the compound glutaraldehyde disinfectant for silkworms of the present invention to disinfect the silkworm rearing room, silkworm rearing tools and the rearing environment for 800 silkworm eggs; after use, it has good disinfection effects on bacterial diseases, viral diseases, pebrine diseases and fungal diseases; it has a relatively small pungent smell and basically no corrosion on silkworm rearing tools such as stainless steel and copper; the incidence of silkworm diseases has decreased, and the incidence of the using farmers' households has decreased by about 4 percentage points on average compared with those without using, and the family farms have decreased by about 5 percentage points on average; the average cocoon yield per sheet has increased by 5% throughout the year.

[0069] The compound glutaraldehyde disinfectant for silkworms of the present invention has the advantages of high efficiency, broad spectrum, thorough disinfection, stable drug efficacy, convenient use, low corrosiveness, and low pungent smell, and has good disinfection effects on bacterial diseases, viral diseases, pebrine diseases and fungal diseases. It is mainly used for disinfecting silkworm rearing rooms, silkworm rearing tools and the environment, and can be used as a substitute for chlorine-containing disinfectants for disinfecting silkworm rearing rooms and silkworm rearing tools.

Claims

1. A compound glutaraldehyde disinfectant for silkworms, characterized in that: Includes glutaraldehyde, didodecyldimethylammonium chloride and trisodium phosphate.

2. A compound glutaraldehyde disinfectant for silkworms according to claim 1, characterized in that: The concentration ratio of each component of the disinfectant is: 2.5% of glutaraldehyde, 0.2% of didodecyl dimethyl ammonium chloride, and 0.3% of trisodium phosphate.

3. A compound glutaraldehyde disinfectant for silkworms according to claim 1, characterized in that: The structural formula of glutaraldehyde is shown below:

4. The method for using the compound glutaraldehyde disinfectant for silkworms according to claim 3, characterized in that: The operation steps are as follows: Step (1): glutaraldehyde, didodecyl dimethyl ammonium chloride and purified water are mixed in proportion to prepare a solution according to the required content, and the solution is placed in bottle A; Step (2): put trisodium phosphate into bottle B; Step (3): When in use, take the contents of bottle A, add a certain amount of water to dissolve, then add the contents of bottle B to continue dissolving, and then perform spray disinfection or immersion disinfection.

5. The method for using the compound glutaraldehyde disinfectant for silkworms according to claim 4, characterized in that: In step (1), the amount of the prepared solution is: 500 ml.

6. The method for using the compound glutaraldehyde disinfectant for silkworms according to claim 4, characterized in that: In step (3), when using, take the contents of bottle A, add 4500 ml of water to dissolve, and then add the contents of bottle B to continue dissolving.

7. The method for using the compound glutaraldehyde disinfectant for silkworms according to claim 4, characterized in that: In step (3), the dissolved mixed solution is used for spraying or immersion disinfection of silkworm room, silkworm tools and silkworm rearing environment.

8. The method for using the compound glutaraldehyde disinfectant for silkworms according to claim 7, characterized in that: The mixed liquid is used to disinfect bacterial diseases, viral diseases, microparticle diseases and fungal diseases in the silkworm room, silkworm tools and silkworm breeding environment.

9. The method for using the compound glutaraldehyde disinfectant for silkworms according to claim 4, characterized in that: In step (3), the spraying amount is 225 ml per square meter.

10. The method for using the compound glutaraldehyde disinfectant for silkworms according to claim 4, characterized in that: In step (3), moisturize for 35 minutes after disinfection.

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