Composition for reducing diarrhea of young ruminants as well as preparation method and application of composition

Abstract

The invention provides a composition for reducing diarrhea of young ruminants as well as a preparation method and application of the composition. The composition comprises a yeast raw material, a dissolution promoter and a carrier, the composition comprises the following components in parts by weight: 5-200 parts of a yeast raw material, 5-10 parts of a dissolution promoter and 10-20 parts of a carrier, wherein the yeast raw materials comprise active dry yeast and yeast cell walls. The carrier comprises one or more of glucose, cane sugar, lactose, microcrystalline cellulose or starch. The feed can solve the problem that diarrhea control products capable of effectively improving diarrhea of young ruminants and meeting feeding requirements of the young ruminants are lacked in the prior art, and is suitable for the field of feeding of the young ruminants.

Description

Technical Field

[0001] The present invention relates to the field of raising young ruminants, and more particularly, to a composition for reducing diarrhea in young ruminants, a preparation method thereof, and an application thereof. Background Art

[0002] In the ruminant breeding industry, especially in the young age stage, the immaturity of its digestive system often leads to a series of health problems, among which diarrhea is one of the most common and worrying diseases. Young ruminants, such as calves, lambs, and fawns, are extremely vulnerable to factors such as feeding management, environmental changes, pathogenic microorganism invasion, nutritional imbalance, and stress due to the special structure and incomplete development of their digestive tracts, resulting in frequent diarrhea. This not only seriously affects the growth and development of young ruminants, increases the mortality rate, but also brings huge economic losses to farmers. Although traditional antibiotic therapy is effective in treating diarrhea in young ruminants, its multi-causal nature leads to weak targeting of antibiotics, easy induction of drug resistance in pathogenic microorganisms, and may remain in livestock products after long-term use, threatening food safety and human health. Therefore, finding alternatives to antibiotics has become a major demand in the breeding industry.

[0003] There are significant differences between the digestive systems of young ruminants and adults, which affects the design and use of diarrhea control products. In adult ruminants, the rumen is the main digestive organ, which processes complex diets through microbial fermentation, while young animals rely on the small intestine to directly digest and absorb nutrients. Therefore, products for adult ruminants often cannot be directly applied to the young age stage, and special consideration needs to be given to formulations that can dissolve in solutions such as water and milk to match the liquid eating habits of young animals, while ensuring the uniformity and nutritional absorption efficiency when the product is mixed into solid feed.

[0004] However, most of the existing diarrhea control products in the prior art are targeted at a single cause or have limitations in the usage scenarios. For example, it is difficult to achieve the dual functions of water solubility and mixing with feed at the same time, which limits their application effects and scope in the actual breeding environment. Especially for young ruminants, there is a lack of a diarrhea relief plan that comprehensively considers the characteristics of their digestive systems and diverse feeding needs. Summary of the Invention

[0005] The main object of the present invention is to provide a composition for reducing diarrhea in young ruminants, a preparation method thereof, and an application thereof, so as to solve the problem in the prior art that there is a lack of a diarrhea control product that can effectively improve diarrhea in young ruminants and meet the feeding needs of young ruminants.

[0006] To achieve the above object, according to the first aspect of the present invention, there is provided a composition for reducing diarrhea in young ruminants, the composition comprising: yeast raw materials, solubilizers and carriers; by weight, the composition comprises 5-200 parts of yeast raw materials, 5-10 parts of solubilizers and 10-20 parts of carriers; wherein, the yeast raw materials include active dry yeast and yeast cell wall; the carriers include one or more of glucose, sucrose, lactose, microcrystalline cellulose or starch.

[0007] Further, the active dry yeast includes one or more of Saccharomyces cerevisiae and / or Candida utilis, Kluyveromyces marxianus or Kluyveromyces lactis; preferably, Saccharomyces cerevisiae includes Saccharomyces cerevisiae var boulardii.

[0008] Further, the yeast raw materials further include one or more of yeast powder, yeast hydrolysate, yeast extract or yeast milk.

[0009] Further, the solubilizer includes maltodextrin and / or cyclodextrin; preferably, the cyclodextrin includes hydroxypropyl-β-cyclodextrin or sulfobutyl-β-cyclodextrin.

[0010] Further, by weight, the composition comprises 10-40 parts of active dry yeast, 20-40 parts of yeast cell wall, 10-20 parts of yeast powder, 5-10 parts of solubilizer and 10-20 parts of carrier; preferably, by weight, the composition comprises 10-40 parts of active dry yeast, 20-40 parts of yeast cell wall, 10-20 parts of yeast powder, 10-20 parts of carrier and 5-10 parts of solubilizer.

[0011] Further, the mass content of protein in the composition is: 15.23% - 20.74%, the number of viable yeast cells ≥ 1.9×10 8 CFU / g, the mass content of mannan is 15% - 20%, and the mass content of β-glucan is 45% - 55%.

[0012] To achieve the above object, according to the second aspect of the present invention, there is provided a preparation method of a composition for reducing diarrhea in young ruminants, the preparation method comprising: mixing yeast raw materials, solubilizers and carriers to obtain a composition; by weight, the addition amount of yeast raw materials is 5-100 parts, the addition amount of solubilizers is 5-10 parts and the addition amount of carriers is 10-20 parts; wherein, the yeast raw materials include active dry yeast and yeast cell wall; the carriers include one or more of glucose, sucrose, lactose, microcrystalline cellulose or starch.

[0013] Further, by weight parts, the addition amount of active dry yeast is 10 - 100 parts, the addition amount of yeast cell wall is 5 - 100 parts, the addition amount of solubilizer is 5 - 10 parts, and the addition amount of carrier is 10 - 20 parts; preferably, the active dry yeast includes one or more of Saccharomyces cerevisiae, Candida utilis, Kluyveromyces marxianus, or Kluyveromyces lactis; preferably, Saccharomyces cerevisiae includes Saccharomyces cerevisiae var boulardii; preferably, the yeast raw material further includes one or more of yeast powder, yeast hydrolysate, yeast extract, or yeast milk; preferably, by weight parts, the addition amount of yeast powder is 10 - 20 parts; preferably, by weight parts, the addition amount of active dry yeast is 10 - 40 parts, the addition amount of yeast cell wall is 20 - 40 parts, the addition amount of yeast powder is 10 - 20 parts, the addition amount of solubilizer is 5 - 10 parts, and the addition amount of carrier is 10 - 20 parts.

[0014] To achieve the above object, according to the third aspect of the present invention, there is provided an application of the above composition for reducing diarrhea in young ruminants, or a composition obtained by the preparation method of the above composition for reducing diarrhea in young ruminants, in feeding young ruminants; the application includes: mixing the composition with the drinking liquid of young ruminants to obtain a first liquid, and feeding the first liquid to young ruminants; the young ruminants include young ruminants of the families Giraffidae, Tragulidae, Moschidae, Cervidae, Antilocapridae, or Bovidae.

[0015] Further, the drinking liquid includes water and / or milk; preferably, the family Bovidae includes the subfamily Bovinae or Caprinae; preferably, when the young ruminant is a young ruminant of the subfamily Bovinae, the addition amount of the composition is 5 - 20 g / h×d; preferably, when the young ruminant is a young ruminant of the subfamily Caprinae, the addition amount of the composition is 3 - 10 g / h×d; preferably, when the young ruminant is a young ruminant of the family Cervidae, the addition amount of the composition is 3 - 10 g / h×d.

[0016] Applying the technical solution of the present invention, in the composition for reducing diarrhea in young ruminants of the present application, components containing active dry yeast and yeast cell wall as yeast raw materials and a carrier capable of providing energy and nutrition are selected. By precisely controlling the proportioning of the yeast raw material, solubilizer, and carrier, the intestinal health of young ruminants is effectively improved, the occurrence of diarrhea is significantly reduced, which helps to improve the survival rate and growth performance of young ruminants, thereby enhancing the breeding efficiency. Detailed Embodiments

[0017] It should be noted that, without conflict, the embodiments in the present application and the features in the embodiments may be combined with each other. The present invention will be described in detail below with reference to the embodiments.

[0018] Glossary of Terms:

[0019] Active dry yeast: It is a dry yeast product obtained by pressing, drying, and dehydrating specially cultured fresh yeast, which still retains strong fermentation ability.

[0020] Saccharomyces boulardii: Also known as Saccharomyces cerevisiae var boulardii, it is a single-celled fungus of the genus Saccharomyces.

[0021] Yeast cell wall: It is a light yellow powdery substance obtained by autolyzing Saccharomyces cerevisiae, followed by enzymatic hydrolysis, centrifugation, and drying.

[0022] Food yeast powder: It is a specially refined food raw material, which is a powdery substance refined by various methods using active yeast as the raw material.

[0023] Young ruminants: Usually refer to ruminants in the transitional stage from birth to reaching adult rumination function. For example, young deer (within 12 months old); lesser mouse-deer (within 6 months old); forest musk deer and alpine musk deer (within 12 months old); pronghorn antelopes before 12 months old, sika deer, red deer, and reindeer (within 12 months old), young water buffalo, dairy cows (within 6 months old), yaks (within 12 months old), goats (within 6 months old), sheep (within 6 months old), and antelopes (within 12 months old).

[0024] As mentioned in the background art, there is a lack of products in the prior art that can effectively improve diarrhea in young ruminants and are also suitable for the feeding scenarios of young ruminants. Based on this, in this application, the inventors attempted to develop a new composition for reducing diarrhea in young ruminants, which can not only effectively improve diarrhea, meet the special digestive needs of young ruminants, but also be suitable for various feeding scenarios, thus proposing a series of protection schemes in this application.

[0025] In the first typical embodiment of the present application, a composition for reducing diarrhea in young ruminants is provided. The composition includes: yeast raw materials, solubilizers, and carriers; the yeast raw materials include active dry yeast and yeast cell walls; by weight parts, the composition includes 5 - 200 parts (including but not limited to 5, 6, 7, 8, 9, 10, 20, 30, 40, 50, 60, 70, 80, 90, 100, 110, 120, 130, 140, 150, 160, 170, 180, 190, or 200 parts) of yeast raw materials, 5 - 10 parts (including but not limited to 5, 6, 7, 8, 9, or 10 parts) of solubilizers, and 10 - 20 parts (including but not limited to 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, or 20 parts) of carriers; wherein, the yeast raw materials include active dry yeast and yeast cell walls; the carriers include one or more of glucose, sucrose, lactose, microcrystalline cellulose, or starch.

[0026] Diarrhea is a common disease in the breeding of young ruminants, which seriously affects the growth of young ruminants, with a relatively high incidence and mortality rate. At home and abroad, antibiotic therapy is often used clinically to treat calf diarrhea. Although it has certain curative effects, there are also many problems that cannot be ignored. First, there are many causes of calf diarrhea, such as improper feeding management, environmental factors, bacterial factors, viral infections, parasitic factors, nutritional deficiency factors, and stress factors, etc. Therefore, in the case of multiple causes, the pertinence of taking antibiotics is not strong, and there is a situation where there is no obvious improvement in diarrhea after taking antibiotics; second, long-term use of antibiotics will cause pathogens to develop drug resistance, resulting in a weakened curative effect of taking antibiotics when young ruminants have diarrhea again during the subsequent breeding process; moreover, taking antibiotics easily leads to excessive antibiotic residues in the final livestock products, threatening food safety and human health. Therefore, finding alternatives to antibiotics has become a major demand in the breeding industry.

[0027] Although there are products in the prior art that can improve the rumen health of ruminants, most of them are for adult ruminants. In the growth process of ruminants, there are significant differences in the digestive systems between the adult and young stages. The stomach of an adult ruminant consists of the rumen, reticulum, omasum, and abomasum. The rumen occupies the vast majority of the total stomach volume and is the core site of microbial fermentation. Its unique rumination process, that is, regurgitating the incompletely digested food in the rumen to the mouth for re-chewing and swallowing, greatly improves the food digestion efficiency, creates favorable conditions for the decomposition of cellulose by microorganisms in the rumen, and promotes the effective conversion of complex nutrients.

[0028] For young ruminants, taking calves and lambs as examples, their stomachs are not fully developed. The rumen and reticulum are not only small in volume but also imperfect in function. Before weaning, they mainly rely on breast milk or milk replacers to obtain nutrition. At this time, the rumen fermentation function is weak and it is difficult to undertake the main digestion task. Food does not go through the rumination process but directly enters the small intestine for digestion and absorption. The small intestine undertakes the important task of the main nutrient absorption organ at this stage to meet the growth and development needs of young ruminants.

[0029] Moreover, because the digestive system of young ruminants is not yet fully developed, liquid feed is needed to meet the nutritional requirements and promote rumen development. Therefore, the proportion of liquid feeding for young ruminants is relatively high. As they grow older, they gradually transition to the feeding mode of adult ruminants mainly based on solid feed. Young ruminants grow and develop rapidly and have high requirements for protein, energy, and minerals, and need high-quality liquid feed and solid feed to support their rapid growth and rumen development. However, most of the existing products for improving rumen health in ruminants are insoluble products, with high application limitations, and their application scenarios and usage are restricted. In addition, there are many types of formula raw materials, the product functions are single, and the supplementation of body electrolytes and energy other than the clinical symptoms of animal diarrhea is not considered. In summary, the existing products for improving rumen health in ruminants are not suitable for improving diarrhea in young ruminants.

[0030] By screening the above-mentioned functional yeast raw materials and the above-mentioned non-yeast source carriers suitable for reducing diarrhea in young ruminants, this application can take into account the adsorption and inhibition of pathogenic bacteria in young ruminants, enhance immunity, supplement electrolytes and energy, effectively improve the diarrhea symptoms of young ruminants, and supplement appropriate nutritional components, making the effect more stable. And this application makes the composition soluble in water, milk, etc. by adding solubilizers and precisely controlling the proportion of each component, which is more convenient for on-site feeding and automated use of young ruminants and more meets the market needs.

[0031] In a preferred embodiment, the active dry yeast includes one or more of Saccharomyces cerevisiae, Candida utilis, Kluyveromyces marxianus, or Kluyveromyces lactis; preferably, Saccharomyces cerevisiae includes Saccharomyces cerevisiae var boulardii; preferably, the yeast raw material also includes one or more of yeast powder, yeast hydrolysate, yeast extract, or yeast milk.

[0032] In a preferred embodiment, the solubilizer includes one or more of maltodextrin, hydroxypropyl-β-cyclodextrin, sulfobutyl-β-cyclodextrin; preferably maltodextrin.

[0033] In a preferred embodiment, by weight parts, the composition comprises 10 - 100 parts (including but not limited to 5, 6, 7, 8, 9, 10, 20, 30, 40, 50, 60, 70, 80, 90 or 100 parts) of active dry yeast, 5 - 100 parts (including but not limited to 5, 6, 7, 8, 9, 10, 20, 30, 40, 50, 60, 70, 80, 90 or 100 parts) of yeast cell wall, 5 - 10 parts of solubilizer and 10 - 20 parts of carrier. Preferably, the composition comprises: 10 - 40 parts of active dry yeast, 20 - 40 parts of yeast cell wall, 10 - 20 parts of yeast powder, 10 - 20 parts of carrier and 5 - 10 parts of solubilizer.

[0034] In the composition of the present application, the contents of active dry yeast, yeast cell wall and solubilizer are controlled within the above ranges, so that the composition can fully exert the functions of adsorbing and inhibiting pathogenic bacteria of young ruminants and improving animal immunity, while supplementing electrolytes and energy, making the effect of improving the diarrhea symptoms of young ruminants more stable, and being applicable to a variety of feeding scenarios, which is more in line with the market needs.

[0035] In a preferred embodiment, the protein content in the composition is 15.23% - 20.74%, the viable yeast cell count ≥ 1.9×10 8 CFU / g, the mannan content is: 15% - 20%, and the β-glucan content is 45% - 55%.

[0036] The protein in the composition of the present application is derived from yeast powder, the viable yeast cell count is derived from active dry yeast, mannan and β-glucan are derived from yeast cell wall and active dry yeast, and the composition of the present application also contains electrolyte substances derived from yeast powder, including substances such as potassium, sodium, sulfur and chlorine, which can supplement electrolytes and nutrients while improving the diarrhea symptoms of young ruminants.

[0037] In the second typical embodiment of the present application, a preparation method of a composition for reducing diarrhea in young ruminants is provided. The preparation method includes: mixing yeast raw materials, solubilizer and carrier to obtain a composition; by weight parts, the addition amount of yeast raw materials is 5 - 100 parts, the addition amount of solubilizer is 5 - 10 parts, and the addition amount of carrier is 10 - 20 parts; wherein, the yeast raw materials include active dry yeast and yeast cell wall; the carrier includes one or more of glucose, sucrose, lactose, microcrystalline cellulose or starch.

[0038] During the preparation of the above composition of the present application, the addition amounts of the yeast raw material, the solubilizer and the carrier are controlled within the above ranges. The composition for reducing diarrhea in young ruminants can be obtained by simple mixing. The composition has simple ingredients and simple preparation steps, can effectively and stably improve the diarrhea symptoms of young ruminants, can also be dissolved in liquids, is applicable to a variety of feeding application scenarios, and is more suitable for market promotion.

[0039] In a preferred embodiment, by weight, the addition amount of active dry yeast is 10-100 parts, the addition amount of yeast cell wall is 5-100 parts, the addition amount of solubilizer is 5-10 parts, and the addition amount of carrier is 10-20 parts; preferably, by weight, the addition amount of yeast powder is 10-20 parts; preferably, by weight, the addition amount of active dry yeast is 10-40 parts, the addition amount of yeast cell wall is 20-40 parts, the addition amount of yeast powder is 10-20 parts, the addition amount of solubilizer is 5-10 parts, and the addition amount of carrier is 10-20 parts.

[0040] In a preferred embodiment, the active dry yeast includes one or more of Saccharomyces cerevisiae, Candida utilis, Kluyveromyces marxianus or Kluyveromyces lactis; preferably, Saccharomyces cerevisiae includes Saccharomyces cerevisiae var boulardii; preferably, the yeast raw material further includes one or more of yeast powder, yeast hydrolysate, yeast extract or yeast milk; preferably, the solubilizer includes maltodextrin and / or cyclodextrin; preferably, the cyclodextrin includes hydroxypropyl-β-cyclodextrin or sulfobutyl-β-cyclodextrin.

[0041] In the third typical embodiment of the present application, there is provided an application of the above composition for reducing diarrhea in young ruminants, or a composition obtained by the preparation method of the above composition for reducing diarrhea in young ruminants, in feeding young ruminants; the application includes mixing the composition with the drinking liquid of young ruminants to obtain a first liquid, and feeding the first liquid to young ruminants; the young ruminants include young ruminants of the families Giraffidae, Tragulidae, Moschidae, Cervidae or Bovidae.

[0042] Preferably, the family Giraffidae includes young giraffes; preferably, the family Tragulidae includes young mouse deer (within 6 months old); preferably, the family Moschidae includes young forest musk deer or alpine musk deer (within 12 months old); preferably, the family Antilocapridae includes pronghorn (within 12 months old); preferably, the family Cervidae includes young sika deer, red deer or reindeer (within 12 months old); preferably, the family Bovidae includes young water buffalo (within 6 months old), young dairy cows (within 6 months old), yaks (within 12 months old), goats (within 6 months old), sheep (within 6 months old), antelopes (within 12 months old).

[0043] The composition of the present application has a dissolution rate maintained at 82.5 - 85.5%, with moderate solubility, and is suitable for use as a mixed feed additive in the drinking water of young ruminants and at the end - use of milk farming. If the solubility is too low to dissolve in the drinking liquid of young ruminants, it cannot be applied to various feeding scenarios. The composition of the present application has moderate solubility, avoiding the above - mentioned two situations, being applicable to various feeding scenarios, helping to improve the diarrhea symptoms of young ruminants, and being more suitable for promotion in the breeding industry.

[0044] When the composition of the present application is applied to the feeding of calves and lambs, it can effectively reduce the diarrhea rate of the target animals by more than 85%. This proves that the composition of the present application can effectively and gently improve the diarrhea symptoms of young ruminants and promote the growth and development of young ruminants.

[0045] In a preferred embodiment, the drinking liquid includes water and / or milk; preferably, the Bovidae family includes the Bovinae subfamily or the Caprinae subfamily; preferably, when the young ruminants include young ruminants of the Bovinae subfamily, the addition amount of the composition is 5 - 20 g / h×d; preferably, when the young ruminants include young ruminants of the Caprinae subfamily, the addition amount of the composition is 3 - 10 g / h×d; preferably, when the young ruminants are young ruminants of the Cervidae family, the addition amount of the composition is 3 - 10 g / h×d.

[0046] Unless otherwise specified, the reagents in the embodiments of the present application are all commercially available products.

[0047] The beneficial effects of the present application will be further explained in detail below with specific embodiments.

[0048] In the present application, the Saccharomyces cerevisiae var boulardii in the embodiments is from Angel Yeast Co., Ltd., with the product number 82000657, the yeast cell wall is from Angel Yeast Co., Ltd., with the product number 82001331; the food yeast powder is from Angel Yeast Co., Ltd., with the product number 82001343.

[0049] Example 1

[0050] The components of the composition for reducing diarrhea in young ruminants in this example include: 30 parts of Saccharomyces cerevisiae var boulardii, 30 parts of yeast cell wall, 10 parts of food yeast powder, 20 parts of anhydrous glucose, and 10 parts of maltodextrin.

[0051] The preparation method is as follows:

[0052] Mix Saccharomyces cerevisiae var boulardii, yeast cell wall, food yeast powder, anhydrous glucose, and maltodextrin to obtain the composition of this example.

[0053] Example 2

[0054] The composition for reducing diarrhea in young ruminants in this example includes: 20 parts of Saccharomyces boulardii, 30 parts of yeast cell wall, 10 parts of food yeast powder, 20 parts of anhydrous glucose, and 20 parts of maltodextrin. The preparation method is exactly the same as that in Example 1.

[0055] Example 3

[0056] The composition for reducing diarrhea in young ruminants in this example includes: 30 parts of Saccharomyces boulardii, 30 parts of yeast cell wall, 10 parts of food yeast powder, 18 parts of anhydrous glucose, and 12 parts of maltodextrin. The preparation method is exactly the same as that in Example 1.

[0057] Example 4

[0058] The only difference between this example and Example 1 is that in the composition for reducing diarrhea in young ruminants in this example, the number of parts of Saccharomyces boulardii is 10 parts, and the preparation method is exactly the same as that in Example 1.

[0059] Example 5

[0060] The only difference between this example and Example 1 is that in the composition for reducing diarrhea in young ruminants in this example, Saccharomyces boulardii is 40 parts, and the preparation method is exactly the same as that in Example 1.

[0061] Example 6

[0062] The only difference between this example and Example 1 is that in the composition for reducing diarrhea in young ruminants in this example, the yeast cell wall is 20 parts, and the preparation method is exactly the same as that in Example 1.

[0063] Example 7

[0064] The only difference between this example and Example 1 is that in the composition for reducing diarrhea in young ruminants in this example, the yeast cell wall is 40 parts, and the preparation method is exactly the same as that in Example 1.

[0065] Example 8

[0066] The only difference between this example and Example 1 is that in the composition for reducing diarrhea in young ruminants in this example, the anhydrous glucose is 10 parts, and the preparation method is exactly the same as that in Example 1.

[0067] Example 9

[0068] The difference between this example and Example 1 is only that the anhydrous glucose in the composition for reducing diarrhea in young ruminants in this example is 30 parts, and the preparation method is exactly the same as that in Example 1.

[0069] Example 10

[0070] The difference between this example and Example 1 is only that the maltodextrin in the composition for reducing diarrhea in young ruminants in this example is 10 parts, and the preparation method is exactly the same as that in Example 1.

[0071] Example 11

[0072] The difference between this example and Example 1 is only that the maltodextrin in the composition for reducing diarrhea in young ruminants in this example is 25 parts, and the preparation method is exactly the same as that in Example 1.

[0073] Comparative Example 1

[0074] The components of the composition for reducing diarrhea in young ruminants in this example include: 30 parts of yeast cell wall, 30 parts of food yeast powder, 20 parts of anhydrous glucose, and 20 parts of maltodextrin. The preparation method is exactly the same as that in Example 1.

[0075] Comparative Example 2

[0076] The components of the composition for reducing diarrhea in young ruminants in this example include: 30 parts of Saccharomyces boulardii, 20 parts of food yeast powder, 20 parts of anhydrous glucose, and 20 parts of maltodextrin.

[0077] Test Example 1

[0078] The compositions prepared in the above examples and comparative examples were measured for protein, viable yeast cell count, mannan oligosaccharide, and β-glucan mass content. The results are shown in Table 1.

[0079] Table 1

[0080] 。

[0081] Test Example 2

[0082] The combinations prepared in the above examples and comparative examples were subjected to solubility detection. The detection method was: Q / AQJM2169s - 2022 / Appendix B, and the detection results are shown in Table 2.

[0083] Table 2

[0084] 。

[0085] Application Example 1

[0086] Select male dairy calves of the same age and weight (before weaning), and randomly divide them into 4 treatment groups (the basal diet group (Dacheng Yongkang Nutrition Technology (Tianjin) Co., Ltd., 8203), the group fed with Composition A of Example 1, the group fed with Composition B of Comparative Example 1, and the group fed with Composition C of Comparative Example 2). Each treatment group has 4 replicates, with 4 calves in each replicate. The experimental period is 8 days. The specific grouping is shown in Table 3.

[0087] Table 3

[0088] 。

[0089] Note: The composition is premixed with 20 g / h×d of powdered raw materials and then added to the milk. The unit "g / h×d" refers to the added amount (g) of the material fed to each animal per day.

[0090] The statistical results of the effect of the composition in this test example on the diarrhea rate of calves are shown in Table 4.

[0091] Table 4

[0092] 。

[0093] Compared with the basal diet group, adding the composition prepared in Example 1 at 20 g / h×d to the diet increased the diarrhea reduction rate by 43.1%; adding the compositions prepared in Comparative Examples 1 and 2 at 20 g / h×d to the diet increased the diarrhea reduction rate by 6.64% and 9.13% respectively.

[0094] In Table 4, the diarrhea rate at 0 d can be regarded as the initial diarrhea situation of the sample young ruminants. At this time, they have not been treated with the composition to be tested. Since the grouping of the sample animals is randomly selected, it is normal for there to be certain differences in the diarrhea rates of each group at 0 d. Statistical analysis shows that there is no significant difference in the diarrhea rates among the groups (the same situation applies in the other application examples).

[0095] The results show that after calves are fed with the composition prepared in the examples of the present invention, compared with those fed only with the basal diet and the compositions prepared in the comparative examples, the diarrhea reduction rate is significantly increased. Feeding the composition prepared in the examples of the present invention can reduce the diarrhea rate of calves.

[0096] Application Example 2

[0097] Select lambs of about 3 months old and the same weight (just weaned), and randomly divide them into 4 treatment groups (the basal diet group (Beijing Zhongbot Biotechnology Engineering Co., Ltd., zbt88), Composition D group, Composition E group, and Composition F group). Each treatment group has 4 replicates, with 4 sheep in each replicate. The experimental period is 8 days. The specific grouping is shown in Table 5 for group feeding in separate pens.

[0098] Table 5

[0099] 。

[0100] Note: After premixing 5 g / h×d of the powdery raw materials of the composition, add them to the drinking water.

[0101] The statistical results of the effect of the composition in this test example on the diarrhea rate of lambs are shown in Table 6.

[0102] Table 6

[0103] 。

[0104] Compared with the basal diet group, when adding the composition prepared in Example 2 at 5 g / h×d to the diet, the diarrhea reduction rate increased by 66.13% respectively; when adding the compositions prepared in Comparative Examples 1 and 2 at 5 g / h×d to the diet respectively, the diarrhea reduction rates increased by 38.16% and 35.16% respectively;

[0105] The results show that: after lambs add the composition prepared in the example of the present invention, compared with only eating the basal diet and the compositions prepared in the comparative examples, the diarrhea reduction rate is significantly increased.

[0106] Application Example 3

[0107] Select young deer with the same body weight and clinical symptoms of diarrhea and randomly divide them into 4 treatment groups (basal diet group (Jilin Maoyuan Feed Co., Ltd., product number 888), Composition G group, Composition H group, Composition I group). Each treatment group has 4 replicates, and each replicate has 10 young deer. The specific grouping is shown in Table 1 and they are raised in separate pens. The test period is 8 days. The specific grouping is shown in Table 7.

[0108] Table 7

[0109] 。

[0110] Note: After premixing 5 g / h×d of the powdery raw materials of the composition, add them to the young deer feed.

[0111] The statistical results of the effect of the composition in this test example on the diarrhea rate of young deer are shown in Table 8.

[0112] Table 8

[0113] 。

[0114] Compared with the control group, when adding the composition prepared in Example 3 at 5 g / h×d to the diet, the diarrhea reduction rate increased by 65.68% respectively; when adding the compositions prepared in Comparative Examples 1 and 2 at 5 g / h×d to the diet respectively, the diarrhea reduction rates increased by 37.17% and 37.48% respectively;

[0115] The results show that: after adding the composition prepared in the embodiment of the present invention, the diarrhea reduction rate of young deer is significantly higher compared to those only fed with the basal diet and the composition prepared in the comparative example.

[0116] From the above description, it can be seen that the above embodiments of the present invention achieve the following technical effects: by screening yeast functional raw materials and non-yeast functional carrier types, the present application can effectively synergize with microorganisms in the body of young ruminants, enhance immunity, supplement electrolytes and energy, and further improve the diarrhea symptoms of young ruminants; moreover, the composition of the present application also contains a solubilizer, and by precisely controlling the above three components, a composition that can be mixed into solid feed and also dissolved in the drinking liquid of young ruminants can be obtained. Compared with the products in the prior art for improving the rumen health / diarrhea symptoms of ruminants, the composition of the present application is rich in components, has a simple preparation method, can be applied to a variety of feeding scenarios, is more suitable for improving the diarrhea of young ruminants, and is more suitable for market promotion.

[0117] The above are only the preferred embodiments of the present invention and are not intended to limit the present invention. For those skilled in the art, the present invention can have various changes and modifications. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included within the protection scope of the present invention.

Claims

1. A composition for reducing diarrhea in young ruminants, characterized in that: The composition comprises: a yeast raw material, a dissolution promoter and a carrier; The composition comprises, by weight, 5-200 parts of the yeast raw material, 5-10 parts of the dissolution agent and 10-20 parts of the carrier; Wherein, the yeast raw material includes active dry yeast and yeast cell wall; The carrier includes one or more of glucose, sucrose, lactose, microcrystalline cellulose or starch.

2. The composition according to claim 1, characterized in that The active dry yeast includes one or more of Saccharomyces cerevisiae, Candida utilis, Kluyveromyces marxianus or Kluyveromyces lactis; The Saccharomyces cerevisiae includes Saccharomyces boulardii.

3. The composition according to claim 1, characterized in that The yeast raw material further comprises one or more of yeast powder, yeast hydrolyzate, yeast extract or yeast milk.

4. The composition according to claim 1, characterized in that The solubilizing agent includes maltodextrin and / or cyclodextrin; The cyclodextrin includes hydroxypropyl-β-cyclodextrin or sulfobutyl-β-cyclodextrin.

5. The composition according to any one of claims 1 to 4, characterized in that In parts by weight, the composition comprises 10-40 parts of the active dry yeast, 20-40 parts of the yeast cell walls, 10-20 parts of the yeast powder, 5-10 parts of the dissolution promoter and 10-20 parts of the carrier.

6. The composition according to claim 1, characterized in that The protein content of the composition is 15.23% to 20.74%, and the number of live yeast cells is ≥ 1.9×10 8 CFU / g, the mass content of mannan is 15%~20%, and the mass content of β-glucan is 45%~55%.

7. A method for preparing a composition for reducing diarrhea in young ruminants, characterized in that: The preparation method comprises: mixing yeast raw materials, a solubilizing agent and a carrier to obtain the composition; In parts by weight, the yeast raw material is added in an amount of 5-100 parts, the dissolution agent is added in an amount of 5-10 parts, and the carrier is added in an amount of 10-20 parts; Wherein, the yeast raw material includes active dry yeast and yeast cell wall; The carrier includes one or more of glucose, sucrose, lactose, microcrystalline cellulose or starch.

8. The preparation method according to claim 7, characterized in that: In parts by weight, the added amount of the active dry yeast is 10-100 parts, the added amount of the yeast cell wall is 5-100 parts, the added amount of the solubilizing agent is 5-10 parts, and the added amount of the carrier is 10-20 parts.

9. Use of the composition for reducing diarrhea in young ruminants according to any one of claims 1 to 6, or the composition obtained by the preparation method of the composition for reducing diarrhea in young ruminants according to any one of claims 7 to 8, in feeding young ruminants; The applications include: mixing the composition with the drinking liquid of the young ruminant to obtain a first liquid, and feeding the first liquid to the young ruminant; The drinking liquid includes water and / or milk; The young ruminant includes a young ruminant of the family Giraffidae, the family Mouse deer, the family Musk deer, the family Cervidae, the family Pronghorn or the family Bovidae.

10. The use according to claim 9, characterized in that: The Bovidae family includes the subfamily Bovinae or Caprinae; When the young ruminant is a young ruminant of the Bovine subfamily, the addition amount of the composition is 5-20 g / h×d; When the young ruminant is a young ruminant of the subfamily Caprinae, the addition amount of the composition is 3-10 g / h×d; When the young ruminant is a young ruminant of the family Cervidae, the added amount of the composition is 3-10 g / h×d.