Preparation method of medicinal and edible health care product based on marine biological enzyme

Abstract

The invention relates to the technical field of microbial enzymes, in particular to a preparation method of a medicinal and edible health care product based on marine biological enzymes, which comprises the following steps: estimating the type and quantity of target enzymes according to production requirements to obtain a first estimation result, performing large-scale production according to the first estimation result, and performing purification and activity protection to obtain the marine biological enzyme-based medicinal and edible health care product. The preparation method comprises the following steps: preparing a first preparation raw material, pre-estimating the variety of target medicinal and edible plants and the number of the target medicinal and edible plants according to production requirements to obtain a second pre-estimation result, performing pretreatment and low-temperature extraction according to the second pre-estimation result to obtain a second preparation raw material, and compounding and mixing the first preparation raw material and the second preparation raw material to obtain a finished product. And detecting the medicinal and edible products, and adjusting the activity protection according to the detection result. The preparation efficiency of the product is improved while the activity of the marine biological enzyme is ensured.

Description

Technical Field

[0001] The present invention relates to the technical field of microbial enzymes, and particularly to a preparation method of a health food homologous to medicine and food based on marine biological enzymes. Background Art

[0002] Homology between medicine and food means that many foods are also drugs, and there is no absolute boundary between them. Ancient medical scientists applied the theories of "four natures" and "five flavors" of traditional Chinese medicine to foods, believing that each food also has "four natures" and "five flavors". In the "Notice on Further Standardizing the Management of Health Food Raw Materials" announced by the Ministry of Health, specific regulations are made on the items homologous to medicine and food, the items that can be used in health foods, and the items prohibited from being used in health foods.

[0003] Chinese Patent Publication No.: CN105533740A discloses a sea cucumber traditional Chinese medicine extract with the efficacy of tonifying the kidney and replenishing essence and its preparation process, belonging to the technical field of marine biological health foods. Using fresh sea cucumbers as raw materials, after enzymatic hydrolysis, they are soaked successively with low-concentration alkaline acid solutions, so that the hydrogen bonds between and within the nutrient molecules of sea cucumbers are cross-linked, and the ionic side chains are opened. After soaking for a certain time, the cross-linking bonds are destroyed and further hydrolyzed into low-molecular forms such as peptone, peptide, and amino acid, which are more easily absorbed by the human body. Among them, the yield of polypeptide reaches more than 12.0%, the yield of polysaccharide reaches more than 14%, and the degree of hydrolysis reaches more than 35%. Reasonably supplemented with traditional Chinese herbs homologous to medicine and food such as Pseudostellaria heterophylla, Atractylodes macrocephala, Poria cocos, Rehmannia glutinosa, Saposhnikovia divaricata, Paeonia lactiflora, Platycladus orientalis, Poria, Astragalus membranaceus, and Chrysanthemum morifolium, the production process is advanced, operable, and the taste of sea cucumbers is improved through a special biological fermentation method, and the nutritional structure is rich. It can be used for insufficient essence and kidney, blood deficiency and physical weakness, soreness and weakness of the waist and knees, abnormal blood lipid, and is an ideal warm-tonic health food. However, this invention does not provide an idea for realizing the quantitative extraction of marine biological enzymes, nor can it solve the problems that the activity of marine biological enzymes decreases due to mixing when mixed with components homologous to medicine and food, and the activity of marine biological enzymes fails due to long-term storage. Summary of the Invention

[0004] Therefore, the present invention provides a preparation method of a health food homologous to medicine and food based on marine biological enzymes to overcome the problems in the prior art that the quantitative extraction of marine biological enzymes is difficult and the production and preparation efficiency of health foods homologous to medicine and food is not high due to the decrease in the activity of marine biological enzymes caused by mixing and long-term storage.

[0005] To achieve the above object, the present invention provides a preparation method of a health food homologous to medicine and food based on marine biological enzymes, including:

[0006] Step S1, estimating the types and quantities of target enzymes according to production requirements to obtain a first estimation result;

[0007] Step S2, perform large-scale production according to the first estimation result in Step S1 to obtain an initial marine bio-enzyme solution;

[0008] Step S3, purify the initial marine bio-enzyme solution to obtain a purified marine bio-enzyme solution;

[0009] Step S4, perform activity protection on the purified marine bio-enzyme solution to obtain a first preliminary raw material;

[0010] Step S5, estimate the types and quantities of target medicinal and edible homologous plants according to production requirements to obtain a second estimation result, and adjust the first estimation result in Step S1 according to the second estimation result;

[0011] Step S6, perform raw material pretreatment on the target medicinal and edible homologous plants according to the second estimation result in Step S5 to obtain pretreated medicinal and edible homologous plants;

[0012] Step S7, perform low-temperature extraction on the pretreated medicinal and edible homologous plants in Step S6 to obtain a second preliminary raw material;

[0013] Step S8, perform compound mixing on the first preliminary raw material and the second preliminary raw material to obtain a medicinal and edible homologous product;

[0014] Step S9, detect the medicinal and edible homologous product, and adjust the activity protection of the purified marine bio-enzyme solution according to the detection result.

[0015] Further, in Step S1, when estimating the types and quantities of target enzymes according to production requirements, input the production requirements into an enzyme production simulation model, and output the types and quantities of target enzymes.

[0016] Further, in Step S1, when estimating the types and quantities of target enzymes according to production requirements, also calculate the actual quantity X1 of the target enzyme based on the unit extraction quantity A of the target enzyme, the production input cost B of the target enzyme, and the unit price S of the unit target enzyme in the actual production situation, and replace the estimated quantity X0 of the target enzyme in the first estimation result with the actual quantity X1 of the target enzyme.

[0017] Further, in Step S2, when performing large-scale production on the first estimation result, perform large-scale production on the first estimation result through a large-scale production method, and the large-scale production method includes:

[0018] Step S201, extract the marine organism genes of the target enzyme type to obtain target genes;

[0019] Step S202: Transfer the target gene into the engineered bacteria, and culture the engineered bacteria under the culture parameters of a culture temperature T1, a culture dissolved oxygen content Q, and a culture pH value R to obtain mature engineered bacteria, where 30°C ≤ T1 ≤ 37°C and Q > 30%.

[0020] Step S203: Dissolve the cell wall of the mature engineered bacteria with lysozyme to obtain a cell solution, and centrifuge the cell solution in a centrifuge to remove cell debris in the cell solution to obtain an initial marine biological enzyme solution.

[0021] Further, in step S3, when purifying the initial marine biological enzyme solution, repeatedly filter the initial marine biological enzyme solution in step S203 through a molecular weight cut-off membrane to obtain a purified marine biological enzyme solution, and detect the concentration p of the purified marine biological enzyme solution until the concentration p of the purified marine biological enzyme solution ≥ 95%.

[0022] Further, in step S4, when performing activity protection on the purified marine biological enzyme solution, perform activity protection on the purified marine biological enzyme solution through an activity protection method, and the activity protection method is as follows:

[0023] Add 5% trehalose and 3% mannitol to the purified marine biological enzyme solution to obtain a freeze-dried powder pre-formulation solution. Place the freeze-dried powder pre-formulation solution in a refrigerator at a freezing temperature T2 for pre-freezing until fine ice crystals are formed. Put the pre-frozen freeze-dried powder pre-formulation solution into a freeze dryer for freeze-drying until a freeze-dried powder without fine ice crystals is obtained. Seal the freeze-dried powder in an empty bottle filled with nitrogen to obtain a first preparation raw material with a first activity k1 > 90%, where T2 = -80°C.

[0024] Further, in step S5, when estimating the types and quantities of target medicinal and edible homologous plants according to production requirements, also calculate the actual quantity Y1 of the target medicinal and edible homologous plants based on the production input cost C1 and the unit price C2 of the target medicinal and edible homologous plants in the actual production situation, and replace the quantity of the target medicinal and edible homologous plants in the second estimation result with the actual quantity Y1 of the target medicinal and edible homologous plants. Calculate the target enzyme demand X2 according to the actual quantity Y1 of the target medicinal and edible homologous plants and the target medicinal and edible homologous plant formula ratio d, compare the target enzyme demand X2 with the actual quantity X1 of the target enzyme, judge the supply and demand status of the target enzyme demand according to the comparison result, and adjust the actual quantity of the target enzyme and the actual quantity of the target medicinal and edible homologous plants according to the judgment result.

[0025] Further, in the step S801, when the first preliminary raw material and the second preliminary raw material are compounded and mixed, the internal temperature Ts of the three-dimensional motion mixer is also monitored in real time by a temperature monitor, and the internal temperature Ts is compared with a preset temperature Ts0. It is set that 25°C ≤ Ts0 ≤ 30°C, and the suitability of the internal temperature is judged according to the comparison result, and the internal temperature of the three-dimensional motion mixer is regulated according to the judgment result.

[0026] Further, in the step S801, when the internal temperature Ts of the three-dimensional motion mixer is monitored in real time by a temperature monitor, the water content Se of the second preliminary raw material is monitored in real time by a near-infrared spectrometer, and the water content Se of the second preliminary raw material is compared with a preset water content Se0. It is set that 5% ≤ Se0 ≤ 10%, and the drying requirement of the second preliminary raw material is judged according to the comparison result, and the judgment process of the suitability of the internal temperature is calibrated according to the judgment result.

[0027] Further, in the step S9, when the medicine and food homologous product is detected and the active protection of the purified marine bio-enzyme solution is adjusted according to the detection result, the activity k2 of the product target enzyme in the medicine and food homologous product is detected by an enzyme activity detection method, and the activity k2 of the product target enzyme is compared with a preset enzyme activity k0, and the qualification of the activity of the product target enzyme is judged according to the comparison result, and the active protection method is adjusted according to the judgment result.

[0028] Compared with the prior art, the beneficial effects of the present invention are as follows. By linking the estimation of the quantity of marine biological enzymes and the estimation of the quantity of medicinal and edible homologous plants, the production plan is dynamically adjusted to avoid waste of raw materials or imbalance between supply and demand, so as to achieve the quantitative extraction of marine biological enzymes. The method also extends the storage period of marine biological enzymes by adopting activity protection, thereby reducing production costs. The method also monitors the compound mixing process of the first prepared raw material and the second prepared raw material, and regulates the internal temperature of the three-dimensional motion mixer according to the water content of the second prepared raw material and the highest suitable temperature of the first prepared raw material, so as to maintain the activity of marine biological enzymes, further improve the product effect, and avoid the problem of reduced activity caused by the mixing of marine biological enzymes and medicinal and edible homologous plants. The method also judges the activity of marine biological enzymes in the medicinal and edible homologous products through the detection of the medicinal and edible homologous products, and adjusts the activity maintenance method of the marine biological enzymes in the medicinal and edible homologous products according to the judgment results, so as to avoid the situation of too large loss of marine biological enzyme activity, resulting in short storage period of unqualified medicinal and edible homologous products, further extend the storage period of marine biological enzymes, ensure the qualification of the supply of marine biological enzymes, be able to mix marine biological enzymes and medicinal and edible homologous plants at any time, improve the production and preparation efficiency of medicinal and edible homologous health products, and thus reduce the cost input while enhancing the economic value of the products. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 FIG. is a schematic flow chart of the preparation method of the medicinal and edible homologous health product based on marine biological enzymes in this embodiment. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0030] In order to make the objectives and advantages of the present invention clearer, the present invention will be further described below in conjunction with embodiments; it should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0031] The preferred embodiments of the present invention will be described below with reference to the accompanying drawings. Those skilled in the art should understand that these embodiments are only used to explain the technical principle of the present invention and do not limit the protection scope of the present invention.

[0032] Please refer to Figure 1 shown, which is a schematic flow chart of the preparation method of the medicinal and edible homologous health product based on marine biological enzymes in this embodiment. The preparation method includes:

[0033] Step S1, estimating the type and quantity of the target enzyme according to the production demand to obtain the first estimation result;

[0034] Step S2, carrying out large-scale production according to the first estimation result in step S1 to obtain the initial marine biological enzyme solution;

[0035] Step S3: Purify the initial marine biological enzyme solution to obtain a purified marine biological enzyme solution;

[0036] Step S4: Protect the activity of the purified marine biological enzyme solution to obtain a first preliminary raw material;

[0037] Step S5: Estimate the types and quantities of target medicinal and edible homologous plants according to production requirements to obtain a second estimation result, and adjust the first estimation result in Step S1 according to the second estimation result;

[0038] Step S6: Pretreat the target medicinal and edible homologous plants according to the second estimation result in Step S5 to obtain pretreated medicinal and edible homologous plants;

[0039] Step S7: Perform low-temperature extraction on the pretreated medicinal and edible homologous plants in Step S6 to obtain a second preliminary raw material;

[0040] Step S8: Compound and mix the first preliminary raw material and the second preliminary raw material to obtain a medicinal and edible homologous product;

[0041] Step S9: Detect the medicinal and edible homologous product, and adjust the activity protection of the purified marine biological enzyme solution according to the detection result.

[0042] Specifically, this method is applied to the intelligent manufacturing control terminal of health products with medicinal and edible homology of marine biological enzymes. Through the linkage of the estimation of the quantity of marine biological enzymes and the estimation of the quantity of medicinal and edible homologous plants, this method dynamically adjusts the production plan to avoid waste of raw materials or imbalance between supply and demand, so as to achieve the quantitative extraction of marine biological enzymes. This method also extends the storage period of marine biological enzymes through activity protection to reduce production costs. This method also monitors the compound mixing process of the first preliminary raw material and the second preliminary raw material, and regulates the internal temperature of the three-dimensional motion mixer according to the water content of the second preliminary raw material and the maximum suitable temperature of the first preliminary raw material to maintain the activity of marine biological enzymes, further improving the product effect and avoiding the problem of reduced activity caused by the mixing of marine biological enzymes and medicinal and edible homologous plants. This method also judges the activity of marine biological enzymes in the medicinal and edible homologous product through the detection of the medicinal and edible homologous product, and adjusts the activity maintenance method of marine biological enzymes in the medicinal and edible homologous product according to the judgment result, avoiding the situation of excessive loss of marine biological enzyme activity, resulting in short storage period of unqualified medicinal and edible homologous products, further extending the storage period of marine biological enzymes, ensuring the qualified supply of marine biological enzymes, enabling the mixing of marine biological enzymes and medicinal and edible homologous plants at any time, and improving the production and preparation efficiency of health products with medicinal and edible homology, thereby enhancing the economic value of the product while reducing the cost investment.

[0043] Specifically, the target enzyme refers to a marine biological enzyme, the type of the target enzyme refers to the type of marine biological enzyme corresponding to the production demand, the target medicinal and edible homologous plant refers to a natural plant that can be used as food and has medicinal value and can regulate the human body functions, such as wolfberry, Chinese yam, and poria cocos, and the type of the target medicinal and edible homologous plant refers to the type of medicinal and edible homologous plant corresponding to the production demand.

[0044] Specifically, in step S1, when estimating the type and quantity of the target enzyme according to the production demand, the production demand is input into the enzyme production simulation model, and the type and quantity of the target enzyme are output.

[0045] Specifically, the enzyme production simulation model refers to a neural network model that takes the production demand as the input and the type and quantity of the target enzyme as the output. The construction process of the enzyme production simulation model includes:

[0046] Dividing 70% of the learning data set into a simulation training set and 30% of the learning data set into a simulation validation set, selecting a recurrent neural network model as the neural network architecture of the enzyme production simulation model, selecting an Adam optimizer and a cross-entropy loss function to train the recurrent neural network model, loading the simulation training set into the recurrent neural network model, performing forward propagation through the recurrent neural network model, calculating the output value of the optimal efficiency simulation model of slurry power generation, calculating the loss function value according to the output value and the true value of the recurrent neural network model, calculating the gradient through the backpropagation algorithm, and updating the weights and biases of the recurrent neural network model. Repeat the processes of forward propagation, calculating the loss, and backpropagation until the preset number of training rounds is reached, and verify the accuracy rate of the recurrent neural network model through the simulation validation set. Output the recurrent neural network model with an accuracy rate meeting 90% as the enzyme production simulation model;

[0047] The production demand refers to the production demand of the medicinal and edible homologous products by the factory according to the actual types and quantities of the products to be produced when producing the medicinal and edible homologous products. The learning data set refers to the data set used to train the enzyme production simulation model. The learning data set includes the recorded historical production demands and the types and quantities of the historical target enzymes corresponding to the historical production demands.

[0048] Specifically, in step S1, when estimating the type and quantity of the target enzyme according to the production demand, the actual quantity X1 of the target enzyme is also calculated according to the unit extraction amount A of the target enzyme, the production input cost B of the target enzyme, and the unit price S of the target enzyme in the actual production situation. Set X1 = B / S × A, and replace the estimated quantity X0 of the target enzyme in the first estimated result with the actual quantity X1 of the target enzyme.

[0049] Specifically, the actual production situation refers to the actual situation in the production of the health food and medicine homologous product based on marine biological enzymes. The target enzyme unit extraction amount refers to the number of target enzymes that can be extracted by one culture tank. The target enzyme production input cost refers to the monetary cost invested in the production of the target enzyme. The unit price of the target enzyme refers to the unit price of purchasing the target enzyme of one culture tank. The actual number of target enzymes refers to the number of enzymes that can be produced under the actual production situation.

[0050] Specifically, in step S2, when carrying out large-scale production on the first estimation result, the first estimation result is subjected to large-scale production through a large-scale production method, and the large-scale production method includes:

[0051] Step S201: Extract the marine biological gene of the target enzyme type to obtain the target gene;

[0052] Step S202: Transfer the target gene into engineering bacteria, and culture the engineering bacteria under the culture parameters of culture temperature T1, culture dissolved oxygen Q, and culture pH value R to obtain mature engineering bacteria, and set 30°C ≤ T1 ≤ 37°C, Q > 30%;

[0053] Step S203: Dissolve the cell wall of the mature engineering bacteria through lysozyme to obtain a cell solution, and centrifuge the cell solution by placing it in a centrifuge to remove cell debris in the cell solution to obtain an initial marine biological enzyme solution.

[0054] Specifically, the engineering bacteria refer to bacteria artificially modified through genetic engineering techniques, such as Escherichia coli. The lysozyme refers to a hydrolase existing in organisms that can specifically decompose peptidoglycan in the cell wall of engineering bacteria to break the cell wall of engineering bacteria. In this embodiment, the specific value of the culture pH value is not limited, and those skilled in the art can set it according to actual needs as long as it meets the culture requirements of the engineering bacteria, and the specific value of the culture pH value can be set according to the type of engineering bacteria.

[0055] Specifically, in step S3, when purifying the initial marine biological enzyme solution, the initial marine biological enzyme solution in step S203 is repeatedly filtered through a molecular weight cut-off membrane to obtain a purified marine biological enzyme solution, and the concentration p of the purified marine biological enzyme solution is detected until the concentration p of the purified marine biological enzyme solution ≥ 95%.

[0056] Specifically, the molecular weight cut-off membrane refers to a separation membrane based on molecular size selectivity, which selectively allows substances with small target enzymes to pass through through physical pore size and chemical structure differences.

[0057] Specifically, in step S4, when performing activity protection on the purified marine biological enzyme solution, the purified marine biological enzyme solution is subjected to activity protection through an activity protection method, and the activity protection method is as follows:

[0058] Add 5% trehalose and 3% mannitol to the purified marine biological enzyme solution to obtain a pre-freeze-dried powder solution. Place the pre-freeze-dried powder solution in a refrigerator at a freezing temperature T2 for pre-freezing until fine ice crystals are formed. Then, put the pre-frozen pre-freeze-dried powder solution into a freeze dryer for freeze-drying until a freeze-dried powder without fine ice crystals is obtained. Pack the freeze-dried powder into an empty bottle filled with nitrogen and seal it to obtain a first preliminary raw material with a first activity k1>90%. Set T2 = -80°C.

[0059] Specifically, the trehalose refers to a naturally occurring non-reducing disaccharide that can protect the target enzyme from maintaining its structural integrity under stresses such as drought, high temperature, and freezing. The mannitol refers to... The freeze dryer, when performing freeze-drying treatment on the pre-frozen pre-freeze-dried powder solution, the freeze-drying treatment steps of the freeze dryer are as follows:

[0060] Step A01, perform primary drying on the pre-frozen pre-freeze-dried powder solution. The temperature Td1 of the first freeze dryer satisfies -45°C ≤ Td1 ≤ -30°C, and the pressure Pd1 of the first freeze dryer satisfies 5 Pa ≤ Pd1 ≤ 20 Pa;

[0061] Step A02, perform secondary drying on the pre-frozen pre-freeze-dried powder solution until a freeze-dried powder without fine ice crystals is obtained. The temperature Td2 of the second freeze dryer satisfies Td2 = 25°C, and the pressure Pd2 of the second freeze dryer satisfies 1 Pa ≤ Pd2 ≤ 5 Pa;

[0062] The first activity refers to the activity of the target enzyme when the freeze-dried powder is packed into an empty bottle filled with nitrogen and sealed.

[0063] Specifically, in step S5, when estimating the types and quantities of target medicinal and edible homologous plants according to production requirements, input the production requirements into a medicinal and edible homologous plant production simulation model, and output the types and quantities of target medicinal and edible homologous plants.

[0064] Specifically, the medicinal and edible homologous plant production simulation model refers to a neural network learning model that takes production requirements as input and outputs the types and quantities of target medicinal and edible homologous plants. The construction process of the medicinal and edible homologous plant production simulation model includes:

[0065] Divide 70% of the simulation dataset into a simulation training set and 30% of the simulation dataset into a simulation validation set. Select a recurrent neural network model as the neural network architecture of the homologous medicinal and edible plant production simulation model. Select the Adam optimizer and the cross-entropy loss function to train the recurrent neural network model. Load the simulation training set into the recurrent neural network model, perform forward propagation through the recurrent neural network model, calculate the output value of the optimal efficiency simulation model of slurry power generation, calculate the loss function value based on the output value and the true value of the recurrent neural network model, calculate the gradient through the backpropagation algorithm, and update the weights and biases of the recurrent neural network model. Repeat the processes of forward propagation, loss calculation, and backpropagation until the preset number of training rounds is reached, and verify the accuracy rate of the recurrent neural network model through the simulation validation set. Output the recurrent neural network model with an accuracy rate meeting 95% as the homologous medicinal and edible plant production simulation model;

[0066] The simulation dataset refers to the dataset used to train the homologous medicinal and edible plant production simulation model. The simulation dataset includes the recorded historical production demands and the types and quantities of the historical target homologous medicinal and edible plants corresponding to the historical production demands.

[0067] Specifically, in step S5, when estimating the types and quantities of the target homologous medicinal and edible plants according to the production demands, the actual quantity Y1 of the target homologous medicinal and edible plants is also calculated based on the production input cost C1 and the unit price C2 of the target homologous medicinal and edible plants in the actual production situation. Set Y1 = C1 / C2, and replace the quantity of the target homologous medicinal and edible plants in the second estimation result with the actual quantity Y1 of the target homologous medicinal and edible plants. Calculate the target enzyme demand X2 according to the actual quantity Y1 of the target homologous medicinal and edible plants and the formula ratio d of the target homologous medicinal and edible plants. Set X2 = (Y1 / d) × (10 - d), compare the target enzyme demand X2 with the actual quantity X1 of the target enzyme, judge the supply and demand status of the target enzyme demand according to the comparison result, and adjust the actual quantity of the target enzyme and the actual quantity of the target homologous medicinal and edible plants according to the judgment result, where:

[0068] When X2 < X1, it is determined that the supply and demand status of the target enzyme demand is supply greater than demand, and the actual quantity of the target enzyme is adjusted. The adjustment method is to replace the actual quantity X1 of the target enzyme with the target enzyme demand X2 to obtain the final production quantity of the target enzyme;

[0069] When X2 = X1, it is determined that the supply and demand status of the target enzyme demand is supply and demand balance, and the actual quantity of the target enzyme and the actual quantity of the target homologous medicinal and edible plants are not adjusted;

[0070] When X2 > X1, it is determined that the supply-demand state of the target enzyme demand is in short supply, and the actual quantity of the target medicinal and edible homologous plants is adjusted. The adjustment plan is to calculate the target enzyme demand Y2 according to the actual quantity X1 of the target enzyme and the formula ratio d of the target medicinal and edible homologous plants, set Y2 = (X1 / (10 - d)) × d, and replace the actual quantity Y1 of the target medicinal and edible homologous plants with the target enzyme demand Y2 to obtain the final production quantity of the target medicinal and edible homologous plants.

[0071] Specifically, the production input cost of the target medicinal and edible homologous plants refers to the monetary cost invested in the production of the target medicinal and edible homologous plants. The unit price of the target medicinal and edible homologous plants refers to the price of a single target medicinal and edible homologous plant. The formula ratio of the target medicinal and edible homologous plants refers to the proportion of the target medicinal and edible homologous plants when the first preparatory raw material and the second preparatory raw material are compounded and mixed. In this embodiment, the specific value of the formula ratio of the target medicinal and edible homologous plants is not limited, and those skilled in the art can set it according to specific needs, as long as it meets the calculation requirements of the target enzyme demand. For example, the specific value of the formula ratio of the target medicinal and edible homologous plants can be limited according to production needs. The supply-demand balance state of the target enzyme demand is whether the actual quantity of the target enzyme meets the demand for the target enzyme. The supply-demand balance state of the target enzyme demand includes that the supply-demand state of the target enzyme demand is in oversupply, the supply-demand state of the target enzyme demand is in balance, and the supply-demand state of the target enzyme demand is in short supply.

[0072] Specifically, in step S6, when preprocessing the raw materials of the target medicinal and edible homologous plants according to the second estimation result in step S5, the target medicinal and edible homologous plants are washed with clean water, and after washing, the target medicinal and edible homologous plants are crushed to obtain the crushed target medicinal and edible homologous plants. The crushed target medicinal and edible homologous plants are placed in an oven at 45°C and dried for 1 hour to obtain the preprocessed medicinal and edible homologous plants.

[0073] Specifically, in step S7, when performing low-temperature extraction on the preprocessed medicinal and edible homologous plants in step S6, the preprocessed medicinal and edible homologous plants are mixed with the extraction solution, and ultrasonic extraction is performed at the ultrasonic temperature T3, ultrasonic power E, and ultrasonic time t1. It is set that 43°C ≤ T3 ≤ 47°C, 200W ≤ E ≤ 400W, and 10min ≤ t1 ≤ 30min to obtain the second preparatory raw material.

[0074] Specifically, the extraction solution refers to the solution used for extracting the pretreated medicinal and edible homologous plants. The extraction solution includes water and ethanol. In this embodiment, the ratio of water to ethanol in the extraction solution is not limited, and those skilled in the art can set it according to the actual situation. For example, the ratio of water to ethanol in the extraction solution can be limited according to the type of the target medicinal and edible homologous plant. The ultrasonic extraction refers to a high-efficiency technology that utilizes the cavitation effect, mechanical vibration, and thermal effect of ultrasonic waves to accelerate the extraction of the target components by the extraction solution.

[0075] Specifically, in step S8, when the first prepared raw material and the second prepared raw material are subjected to compounding and mixing treatment, the first prepared raw material and the second prepared raw material are subjected to compounding and mixing treatment by a compounding and mixing treatment method. The compounding and mixing treatment method includes:

[0076] Step S801: Place the second prepared raw material into a three-dimensional motion mixer and start the three-dimensional motion mixer for stirring. During the stirring process, slowly add the first prepared raw material, and detect the mixing uniformity CV of the first prepared raw material and the second prepared raw material by a near-infrared spectrometer. The three-dimensional motion mixer stirs the first prepared raw material and the second prepared raw material until the mixing uniformity CV ≤ 5%, and a mixed powder is obtained.

[0077] Step S802: Mix the mixed powder with the tea base and then pack it into a filter paper bag to obtain a medicinal and edible homologous product.

[0078] Specifically, the three-dimensional motion mixer refers to a device that realizes efficient and uniform mixing of materials through multi-directional motion. The near-infrared spectrometer refers to an optical analysis instrument based on the interaction of substances in the near-infrared light region. By detecting the absorption, reflection, or transmission characteristics of the sample to near-infrared light, it can quickly and non-destructively analyze the chemical composition and physical properties of the substance, and is used to detect the mixing uniformity of the first prepared raw material and the second prepared raw material. The tea base refers to the selected tea in the components of the medicinal and edible homologous product, such as black tea and green tea. The filter paper bag refers to the container used to package the mixed powder and the tea base.

[0079] Specifically, in step S801, when the first prepared raw material and the second prepared raw material are subjected to compounding and mixing treatment, the internal temperature Ts of the three-dimensional motion mixer is also monitored in real time by a temperature monitor, and the internal temperature Ts is compared with a preset temperature Ts0. It is set that 25°C ≤ Ts0 ≤ 30°C, and the suitability of the internal temperature is judged according to the comparison result, and the internal temperature of the three-dimensional motion mixer is regulated according to the judgment result, where:

[0080] When Ts ≤ Ts0, it is determined that the suitability of the internal temperature is suitable, and the internal temperature of the three-dimensional motion mixer is not regulated.

[0081] When Ts > Ts0, it is determined that the internal temperature is unsuitable, and the internal temperature of the three-dimensional motion mixer is regulated. The regulation method is to reduce the water temperature of the cooling jacket of the three-dimensional motion mixer until Ts ≤ Ts0.

[0082] Specifically, the temperature monitor refers to a machine used to monitor the internal temperature of the three-dimensional motion mixer. The preset temperature refers to a preset value used to judge the suitability of the internal temperature. The suitability of the internal temperature refers to whether the internal temperature of the three-dimensional motion mixer is suitable for mixing the first prepared raw material and the second prepared raw material. The suitability of the internal temperature includes that the internal temperature is suitable and the internal temperature is unsuitable. The cooling jacket of the three-dimensional motion mixer refers to a device in the three-dimensional motion mixer used to control the temperature of the material during the mixing process.

[0083] Specifically, in step S801, when the internal temperature Ts of the three-dimensional motion mixer is monitored in real time by a temperature monitor, the water content Se of the second prepared raw material is monitored in real time by a near-infrared spectrometer, and the water content Se of the second prepared raw material is compared with the preset water content Se0. It is set that 5% ≤ Se0 ≤ 10%. According to the comparison result, the drying requirement of the second prepared raw material is judged, and the judgment process of the suitability of the internal temperature is calibrated according to the judgment result, where:

[0084] When Se ≤ Se0, it is determined that the drying requirement of the second prepared raw material is not required, and the judgment process of the suitability of the internal temperature is not calibrated;

[0085] When Se > Se0, it is determined that the drying requirement of the second prepared raw material is required, and the judgment process of the suitability of the internal temperature is calibrated. Through the calibration coefficient f = 1.3 - 0.3×e -(Se-Se0) The preset temperature is calibrated to obtain the calibrated preset temperature Ts0f. It is set that Ts0f = Ts0 × f. The internal temperature Ts is compared with the calibrated preset temperature Ts0f again, and the suitability of the internal temperature is judged according to the comparison result, and the internal temperature of the three-dimensional motion mixer is regulated according to the judgment result.

[0086] Specifically, the water content of the second prepared raw material refers to the proportion of the water content in the second prepared raw material. The preset water content refers to a preset value used to judge the drying requirement of the second prepared raw material. The drying requirement of the second prepared raw material refers to the situation of whether the second prepared raw material needs to be dried during the stirring process. The drying requirement of the second prepared raw material includes that the drying requirement of the second prepared raw material is not required and the drying requirement of the second prepared raw material is required.

[0087] Specifically, in step S801, during the calibration of the process of judging the suitability of the internal temperature, the highest active temperature Th of the first preparatory raw material is compared with the preset temperature Ts0f after calibration, and the temperature adaptation of the target enzyme is judged according to the comparison result, and the preset temperature after calibration is revised according to the judgment result, where:

[0088] When Th≥Ts0f, it is determined that the temperature adaptation of the target enzyme is suitable, and the preset temperature after calibration is not revised;

[0089] When Th<Ts0f, it is determined that the temperature adaptation of the target enzyme is not suitable, and the preset temperature after calibration is revised. The revision plan is to replace the preset temperature Ts0f after calibration with the highest active temperature Th, and compare the internal temperature Ts with the highest active temperature Th, and judge the suitability of the internal temperature according to the comparison result, and control the internal temperature of the three-dimensional motion mixer according to the judgment result.

[0090] Specifically, the highest active temperature of the first preparatory raw material refers to the highest temperature at which the target enzyme in the first preparatory raw material can maintain its activity and adapt, and the temperature adaptation of the target enzyme refers to the situation where the target enzyme can adapt to the preset temperature after calibration. The temperature adaptation of the target enzyme includes that the temperature adaptation of the target enzyme is suitable and the temperature adaptation of the target enzyme is not suitable.

[0091] Specifically, in step S9, when detecting the medicated and edible homologous products and adjusting the activity protection of the purified marine biological enzyme solution according to the detection results, the activity k2 of the product target enzyme in the medicated and edible homologous products is detected by an enzyme activity detection method, and the activity k2 of the product target enzyme is compared with the preset enzyme activity k0, and the qualification of the activity of the product target enzyme is judged according to the comparison result, and the activity protection method is adjusted according to the judgment result, where:

[0092] When k2≥k0, it is determined that the qualification of the activity of the product target enzyme is qualified, and the activity protection method is not adjusted;

[0093] When k2<k0, it is determined that the qualification of the activity of the product target enzyme is unqualified, and the activity protection method is adjusted to adopt a double-layer embedding method for activity protection. The double-layer embedding method includes:

[0094] Step A1, dissolve trehalose in deionized water, add the purified marine biological enzyme solution, mix evenly and dry to obtain trehalose-enzyme particles. Also dissolve chitosan powder in 1% acetic acid solution and stir until it becomes a transparent colloid to obtain a chitosan solution;

[0095] Step A2: Immerse the trehalose-enzyme particles in the chitosan solution, and dry them at 40°C in a vacuum for 6 - 12 hours. Repeat this process 2 - 3 times to obtain the adjusted first preliminary raw material;

[0096] And replace the first preliminary raw material with the adjusted first preliminary raw material.

[0097] Specifically, the activity of the target enzyme of the product refers to the activity of the target enzyme in the product of homologous medicine and food. The preset enzyme activity refers to the preset value used to judge whether the activity of the target enzyme of the product is qualified. In this embodiment, the specific value of the preset enzyme activity is not limited, and those skilled in the art can set it according to their needs. For example, the specific value of the preset enzyme activity can be limited according to the type of the target enzyme. The qualified situation of the activity of the target enzyme of the product refers to whether the activity of the target enzyme of the product reaches the preset standard. The qualified situation of the activity of the target enzyme of the product includes that the qualified situation of the activity of the target enzyme of the product is qualified and the qualified situation of the activity of the target enzyme of the product is unqualified.

[0098] So far, the technical solution of the present invention has been described in conjunction with the preferred embodiments shown in the drawings. However, it is easy for those skilled in the art to understand that the protection scope of the present invention is obviously not limited to these specific embodiments. Without departing from the principle of the present invention, those skilled in the art can make equivalent changes or substitutions to the relevant technical features, and the technical solutions after these changes or substitutions will fall within the protection scope of the present invention.

Claims

1. A method for preparing a medical and edible health product based on marine enzymes, characterized in that: include: Step S1, estimating the type and quantity of the target enzyme according to production requirements to obtain a first estimation result; Step S2, performing large-scale production according to the first estimated result in step S1 to obtain an initial marine biological enzyme solution; Step S3, purifying the initial marine biological enzyme solution to obtain a purified marine biological enzyme solution; Step S4, protecting the activity of the purified marine biological enzyme solution to obtain a first preparatory raw material; Step S5, estimating the types and quantity of the target medicinal and edible plants according to production requirements, obtaining a second estimation result, and adjusting the first estimation result in step S1 according to the second estimation result; Step S6, pre-processing the target medicinal and edible plant according to the second estimated result in step S5 to obtain a pre-processed medicinal and edible plant; Step S7, performing low-temperature extraction treatment on the pretreated medicinal and edible plants in step S6 to obtain a second prepared raw material; Step S8, compounding and mixing the first prepared raw material and the second prepared raw material to obtain a medicine and food product; Step S9, testing the food-medicine homologous product, and adjusting the activity protection of the purified marine enzyme solution according to the test results.

2. The method for preparing the marine enzyme-based health product of claim 1, characterized in that: In the step S1, when estimating the type and quantity of the target enzyme according to the production demand, the production demand is input into the enzyme production simulation model, and the type and quantity of the target enzyme are output.

3. The method for preparing the marine enzyme-based health product of claim 2, characterized in that: In step S1, when estimating the type and quantity of the target enzyme according to production requirements, the actual quantity X1 of the target enzyme is also calculated based on the unit extraction amount A of the target enzyme, the production input cost B of the target enzyme and the unit price S of the target enzyme in the actual production situation, and the estimated quantity X0 of the target enzyme in the first estimation result is replaced by the actual quantity X1 of the target enzyme.

4. The method for preparing the marine enzyme-based health product of claim 3, characterized in that: In the step S2, when the first estimation result is mass-produced, the first estimation result is mass-produced by a mass-production method, and the mass-production method includes: Step S201, extracting the marine biological gene of the target enzyme type to obtain the target gene; Step S202, transferring the target gene into the engineered bacteria, and culturing the engineered bacteria under the culture parameters of culture temperature T1, culture dissolved oxygen content Q and culture pH value R to obtain mature engineered bacteria, set at 30°C≤T1≤37°C, Q>30%; Step S203, dissolving the cell wall of the mature engineered bacteria by lysing bacteria to obtain a cell solution, and placing the cell solution in a centrifuge for centrifugation to remove cell debris in the cell solution to obtain an initial marine biological enzyme solution.

5. The method for preparing the marine enzyme-based health product of claim 4, characterized in that: In step S3, when the initial marine enzyme solution is purified, the initial marine enzyme solution in step S203 is repeatedly filtered through a molecular weight cutoff membrane to obtain a purified marine enzyme solution, and the concentration p of the purified marine enzyme solution is detected until the concentration p of the purified marine enzyme solution is ≥95%.

6. The method for preparing the marine enzyme-based health product of claim 5, characterized in that: In the step S4, when the purified marine biological enzyme solution is protected for activity, the purified marine biological enzyme solution is protected for activity by an activity protection method, wherein the activity protection method is: Add 5% trehalose and 3% mannitol to the purified marine enzyme solution to obtain a freeze-dried powder pre-liquid, place the freeze-dried powder pre-liquid in a refrigerator with a freezing temperature of T2 for pre-freezing until fine ice crystals are formed, place the pre-frozen freeze-dried powder pre-liquid in a freeze dryer for freeze-drying until a freeze-dried powder without fine ice crystals is obtained, and place the freeze-dried powder in an empty bottle filled with nitrogen and seal it to obtain a first preparatory raw material with a first activity k1>90%, and set T2=-80°C.

7. The method for preparing the marine enzyme-based health product of claim 6, characterized in that: In the step S5, when estimating the types and quantity of the target medicinal and edible plants according to the production demand, the actual quantity Y1 of the target medicinal and edible plants is calculated according to the production input cost C1 of the target medicinal and edible plants and the unit price C2 of the target medicinal and edible plants in the actual production situation, and the target medicinal and edible plants in the second estimated result are replaced with the actual quantity Y1 of the target medicinal and edible plants, and the target enzyme demand X2 is calculated according to the actual quantity Y1 of the target medicinal and edible plants and the formula ratio d of the target medicinal and edible plants, and the target enzyme demand X2 is compared with the actual quantity X1 of the target enzyme, and the supply and demand state of the target enzyme demand is judged according to the comparison result, and the actual quantity of the target enzyme and the actual quantity of the target medicinal and edible plants are adjusted according to the judgment result.

8. The method for preparing the marine enzyme-based health product of claim 7, characterized in that: In the step S801, when the first prepared raw material and the second prepared raw material are compounded and mixed, the internal temperature Ts of the three-dimensional motion mixer is also monitored in real time by a temperature monitor, and the internal temperature Ts is compared with the preset temperature Ts0, and 25°C≤Ts0≤30°C is set, and the suitability of the internal temperature is judged based on the comparison result, and the internal temperature of the three-dimensional motion mixer is regulated based on the judgment result.

9. The method for preparing the marine enzyme-based health product of claim 8, characterized in that: In the step S801, when the internal temperature Ts of the three-dimensional motion mixer is monitored in real time by the temperature monitor, the water content Se of the second prepared raw material is monitored in real time by the near-infrared spectrometer, and the water content Se of the second prepared raw material is compared with the preset water content Se0, and 5%≤Se0≤10% is set, and the drying requirement of the second prepared raw material is judged according to the comparison result, and the judgment process of the suitability of the internal temperature is calibrated according to the judgment result.

10. The method for preparing the marine enzyme-based health product of claim 9, characterized in that: In step S9, when the medicine-food product is tested and the activity protection of the purified marine biological enzyme solution is adjusted according to the test result, the activity k2 of the target enzyme in the medicine-food product is tested by an enzyme activity detection method, and the activity k2 of the target enzyme is compared with the preset enzyme activity k0, and the qualified activity of the target enzyme is judged according to the comparison result, and the activity protection method is adjusted according to the judgment result.

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