Phytobacterium plantarum with bacteriostatic activity not affected by water-absorbent resin and application of phytobacterium plantarum
By using specific plant Bacillus Lactobacillus 021 mixed with water-absorbent resin, the problem of reducing growth activity of Lactobacillus in the water-absorbent resin environment and inhibiting pathogenic bacteria activity is solved, and the goal of maintaining antibacterial effects in sanitary products is achieved.
Patent Information
- Application Number
- CN202510276266.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-03-10
- Publication Date
- 2025-06-10
AI Technical Summary
In the prior art, when Lactobacillus is mixed with a water-absorbent resin, the growth activity of Lactobacillus and the inhibitory activity of pathogenic bacteria will be affected, resulting in a decrease in the antibacterial effect of sanitary products.
The specific Lactiplantibacillus plantarum was used. After mixing with water-absorbing resin, the strain was grown in MRS liquid culture medium, maintaining good growth activity, and effectively inhibiting the growth of Candida albicans, E. coli and Staphylococcus aureus.
After mixing Lactobacillus plantarum 021 with water-absorbent resin, it maintains good growth activity and inhibits the activity of pathogenic bacteria. It can be effectively applied to sanitary napkins and other sanitary products, reduces the risk of infection and provides protection for women's health.
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Figure CN120118783A_ABST
Abstract
Description
Technical Field
[0001] The present invention relates to the field of microbial applications, involving Lactiplantibacillus plantarum and its applications, and particularly to a Lactiplantibacillus plantarum whose antibacterial activity is not affected by superabsorbent resin and its application in the preparation of sanitary products. Background Art
[0002] Infectious vaginitis is a highly prevalent disease that affects women's health globally. Common pathogenic bacteria include Candida albicans, Staphylococcus aureus, Escherichia coli, Gardnerella vaginalis, etc. Lactobacilli are the dominant flora in the vaginal microecosystem of healthy reproductive-age women. They maintain the acidic environment of the vagina by producing lactic acid, inhibit the growth of harmful bacteria, and at the same time promote the repair of the vaginal mucosal barrier and enhance the local resistance of the vagina. Oral or vaginal topical use of female probiotic preparations mainly containing Lactobacilli can improve the vaginal microecological imbalance in women and assist in the treatment and prevention of infectious vaginitis. The Lactobacilli contained in female probiotic preparations mainly include Lactobacillus crispatus, Lactobacillus reuteri, and Lactiplantibacillus plantarum, etc.
[0003] Superabsorbent resin is a type of high-molecular compound with a certain degree of crosslinking, insoluble in water, and having high water swelling properties. Currently, more than 80% of the total superabsorbent resin produced in the world is used in health care and cosmetics. Superabsorbent resin is an important raw material for the production of products such as sanitary napkins, tampons, and diapers.
[0004] In recent years, antibacterial sanitary napkins have been favored by more and more women. During menstruation, since blood is a good medium for bacteria, it is easy to breed bacteria and increase the risk of infection. Antibacterial sanitary napkins contain antibacterial components that can inhibit the growth of pathogenic bacteria and reduce the risk of infectious diseases. Based on the inhibitory effect of Lactobacilli on common pathogenic bacteria, there are already tampon products containing Lactobacilli abroad to provide health protection for women.
[0005] The preparation of sanitary products containing Lactobacilli requires that Lactobacilli have good compatibility with the materials used to produce sanitary products, that is, the growth activity and antibacterial activity of Lactobacilli will not decline due to the influence of superabsorbent resin.
[0006] Through retrieval, no patent publication documents related to the present invention patent application have been found. Summary of the Invention
[0007] The purpose of the present invention is to overcome the above-mentioned deficiencies in the prior art and provide a Lactiplantibacillus plantarum whose antibacterial activity is not affected by superabsorbent resin and its application.
[0008] The technical solution adopted by the present invention to solve its technical problems is:
[0009] A Lactiplantibacillus plantarum whose antibacterial activity is not affected by superabsorbent resin (the original name of Lactiplantibacillus plantarum was Lactobacillus plantarum, with the Latin name Lactobacillus plantarum. According to the 4th announcement of the National Health Commission in 2022, it was renamed Lactiplantibacillus plantarum, with the Latin name Lactiplantibacillus plantarum). The name of the Lactiplantibacillus plantarum is: Lactobacillus 021, the classification name is: Lactobacillus plantarum, the preservation number is: CGMCC No. 22169, the preservation date is: April 12, 2021, and the preservation unit is: General Microbiology Center of the China Committee for Culture Collection of Microorganisms, Institute of Microbiology, Chinese Academy of Sciences, No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing.
[0010] Furthermore, Lactobacillus 021 was mixed with superabsorbent resin and cultured in MRS liquid medium, and the growth activity of Lactobacillus 021 was not inhibited by the superabsorbent resin.
[0011] Furthermore, Lactobacillus 021 was co-cultured with Candida albicans, and Lactobacillus 021 inhibited the growth of Candida albicans; Lactobacillus 021 was mixed with superabsorbent resin and then co-cultured with Candida albicans, and the inhibitory activity of Lactobacillus 021 against Candida albicans was not affected by the superabsorbent resin.
[0012] Furthermore, Lactobacillus 021 was co-cultured with Staphylococcus aureus, and Lactobacillus 021 inhibited the growth of Staphylococcus aureus; Lactobacillus 021 was mixed with superabsorbent resin and then co-cultured with Staphylococcus aureus, and the inhibitory activity of Lactobacillus 021 against Staphylococcus aureus was not affected by the superabsorbent resin.
[0013] Furthermore, Lactobacillus 021 was co-cultured with Escherichia coli, and Lactobacillus 021 inhibited the growth of Escherichia coli; Lactobacillus 021 was mixed with superabsorbent resin and then co-cultured with Escherichia coli, and the inhibitory activity of Lactobacillus 021 against Escherichia coli was not affected by the superabsorbent resin.
[0014] Applications of the above-mentioned Lactobacillus 021 mixed with superabsorbent resin in feminine hygiene products such as sanitary napkins and tampons.
[0015] Applications of the above-mentioned Lactobacillus 021 mixed with superabsorbent resin in other sanitary products.
[0016] The advantages and positive effects achieved by the present invention are:
[0017] The Lactobacillus 021 of the present invention still has good growth activity after being mixed with superabsorbent resin.
[0018] After being mixed with a water-absorbing resin, Lactiplantibacillus plantarum 021 of the present invention still has strong inhibitory effects on common pathogenic bacteria, such as Candida albicans, Escherichia coli, and Staphylococcus aureus. Therefore, after being mixed with a water-absorbing resin, Lactiplantibacillus plantarum 021 can be applied to the preparation of sanitary products such as sanitary napkins to inhibit pathogenic bacteria, reduce the risk of infection, and provide protection for women's health. BRIEF DESCRIPTION OF THE DRAWINGS
[0019] Figure 1 It is a growth activity diagram of Lactiplantibacillus plantarum 021 after being mixed with a water-absorbing resin in the present invention;
[0020] Figure 2 It is an antibacterial activity diagram of Lactiplantibacillus plantarum 021 against Candida albicans after being mixed with a water-absorbing resin in the present invention;
[0021] Figure 3 It is an antibacterial activity diagram of Lactiplantibacillus plantarum 021 against Staphylococcus aureus after being mixed with a water-absorbing resin in the present invention;
[0022] Figure 4 It is an antibacterial activity diagram of Lactiplantibacillus plantarum 021 against Escherichia coli after being mixed with a water-absorbing resin in the present invention. DETAILED DESCRIPTION OF THE INVENTION
[0023] The present invention will be described in detail below with reference to specific embodiments. It should be noted that these embodiments are narrative and not restrictive, and the protection scope of the present invention cannot be limited thereby.
[0024] The raw materials used in the present invention are all conventional commercially available products without special instructions; the methods used in the present invention are all conventional methods in the art without special instructions.
[0025] A Lactiplantibacillus (formerly named Lactobacillus plantarum, with the Latin name Lactobacillus plantarum, and renamed Lactiplantibacillus plantarum according to the 4th announcement of the National Health Commission in 2022, with the Latin name Lactiplantibacillus plantarum) whose antibacterial activity is not affected by a water-absorbing resin. The name of the Lactiplantibacillus is Lactiplantibacillus plantarum 021, the classification name is Lactobacillus plantarum, the preservation number is CGMCC No. 22169, the preservation date is April 12, 2021, and the preservation unit is the General Microbiology Center of the China Committee for Culture Collection of Microorganisms, Institute of Microbiology, Chinese Academy of Sciences, No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing.
[0026] Example 1: The growth activity of Lactiplantibacillus plantarum 021 is not affected by a water-absorbing resin
[0027] (1) Resuscitation and culture of Lactobacillus plantarum 021
[0028] Take out the Lactobacillus plantarum 021 strain stored in the -80°C refrigerator, use an inoculation loop to streak on the suitable solid MRS medium by the three-zone streaking method. After completion, invert it and place it in an incubator for 48 h. Then, in a laminar flow hood, use a pipette to aspirate 5 mL of the suitable MRS medium into a sterile test tube. After picking a single colony of Lactobacillus plantarum 021 and inoculating it, place it on a shaker at 37°C for 12 h. Take the bacterial liquid from the previous step and inoculate it into the test tube with the added medium at an inoculation amount of 2%, and continue to culture it in an incubator at 37°C for 12 h. Use the medium to adjust the bacterial liquid concentration to 1×10 9 CFU / mL.
[0029] (2) Preparation of water-absorbing resin
[0030] Weigh 0.1 g of water-absorbing resin, place it in a 50 mL centrifuge tube, add 25 mL of normal saline, and fully swell it at room temperature for 12 h. Put the prepared sample into an autoclave and sterilize it at 121°C for 30 min for later use.
[0031] (3) Mixing and incubating water-absorbing resin with Lactobacillus plantarum 021
[0032] Add 4 mL of MRS medium and 1 mL of Lactobacillus plantarum 021 bacterial liquid to the sterilized and cooled water-absorbing resin. The blank control does not add resin. After placing it on a shaker at 37°C and oscillating for 12 h, take 1 mL of the bacterial liquid, dilute it by 10-fold gradient, and take 50 μL of the diluted bacterial liquid and spread it on an MRS plate. 10 -5 ,10 -6 ,10 -7 Make three replicates for each dilution, culture it in an incubator at 37°C for 24 h, and observe the growth of Lactobacillus plantarum 021 colonies.
[0033] The results are as Figure 1 shown. There is no obvious difference in the number of colonies of Lactobacillus plantarum 021 growing on the MRS plate after mixing and incubating with the water-absorbing resin and the control group of Lactobacillus plantarum 021 incubated alone, indicating that the growth activity of Lactobacillus plantarum 021 is not affected by the water-absorbing resin.
[0034] Example 2: Lactobacillus plantarum 021 inhibits Candida albicans and its antibacterial activity is not affected by water-absorbing resin
[0035] (1) Resuscitation and culture of Candida albicans and Lactobacillus plantarum 021
[0036] Take out the bacterial strains stored in the -80°C refrigerator, and use an inoculation loop to streak on the appropriate solid medium (Candida albicans on Sabouraud medium, Lactobacillus plantarum 021 on MRS medium) using the three-zone streaking method. After completion, invert and place it in an incubator and culture for 48 h. In a laminar flow hood, use a pipette to aspirate 5 mL of the appropriate medium into a sterilized test tube, and then pick a single colony and inoculate it into the test tube with the appropriate medium. Culture Lactobacillus plantarum 021 in a shaker at 37°C for 12 h, and culture Candida albicans in a shaker at 30°C for 20 h. Inoculate the bacterial liquid from the previous step into a new medium at an inoculation amount of 2% and passage it once. Use a blank medium to adjust the bacterial liquid concentration to 1×10 9 CFU / mL.
[0037] (2) Preparation of water-absorbing resin
[0038] Weigh 0.1 g of water-absorbing resin, place it in a 50 mL centrifuge tube, add 25 mL of normal saline, and fully swell it at room temperature for 12 h. Put the prepared sample into an autoclave and sterilize it at 121°C for 30 min for later use.
[0039] (3) Mixing and incubating water-absorbing resin with Lactobacillus plantarum 021 and Candida albicans
[0040] Add 3 mL of MRS medium, 1 mL of Lactobacillus plantarum 021 bacterial liquid, and 1 mL of Candida albicans bacterial liquid to the sterilized and cooled water-absorbing resin. The control groups are the co-incubation of Lactobacillus plantarum 021 and Candida albicans and the single incubation of Candida albicans. After culturing in a shaker at 37°C for 20 h, take 1 mL of the bacterial liquid, dilute it by 10-fold gradient, and take 50 μL of the diluted bacterial liquid and spread it on a Sabouraud medium plate. 10 -5 ,10 -6 ,10 -7 For each dilution, make three replicates, culture in an incubator at 30°C for 48 h, and observe the growth of Candida albicans colonies.
[0041] The results are as Figure 2 shown. The number of Candida albicans colonies growing on the Sabouraud medium plate after the mixed incubation of Lactobacillus plantarum 021, Candida albicans, and water-absorbing resin has no obvious difference from the control group of the co-incubation of Lactobacillus plantarum 021 and Candida albicans, and is significantly less than the number of colonies in the control group of the single incubation of Candida albicans, indicating that Lactobacillus plantarum 021 inhibits the growth of Candida albicans and its antibacterial activity is not affected by the water-absorbing resin.
[0042] Example 3: Lactobacillus plantarum 021 inhibits Staphylococcus aureus and its antibacterial activity is not affected by water-absorbing resin
[0043] (1) Resuscitation and culture of Staphylococcus aureus and Lactobacillus plantarum 021
[0044] Take out the bacterial strains stored in the -80 °C refrigerator, and use an inoculation loop to streak on the appropriate solid medium (Staphylococcus aureus on LB medium, Lactobacillus plantarum 021 on MRS medium) using the three-zone streaking method. After completion, place it upside down in an incubator and incubate for 48 h. In a laminar flow hood, use a pipette to aspirate 5 mL of the appropriate medium into a sterilized test tube, and then pick a single colony and inoculate it into the test tube containing the appropriate medium, and place it on a shaker at 37 °C for 12 h. Take the bacterial liquid from the previous step and inoculate it into a new medium at an inoculation amount of 2% for one passage. Use a blank medium to adjust the bacterial liquid concentration to 1×10 9 CFU / mL.
[0045] (2) Preparation of superabsorbent resin
[0046] Weigh 0.1 g of superabsorbent resin, place it in a 50 mL centrifuge tube, add 25 mL of normal saline, and fully swell it at room temperature for 12 h. Put the prepared sample into an autoclave and sterilize it at 121 °C for 30 min for later use.
[0047] (3) Mixing and incubating superabsorbent resin with Lactobacillus plantarum 021 and Staphylococcus aureus
[0048] Add 3 mL of MRS medium, 1 mL of Lactobacillus plantarum 021 bacterial liquid, and 1 mL of Staphylococcus aureus bacterial liquid to the sterilized and cooled superabsorbent resin. After incubating on a shaker at 37 °C for 12 h, take 1 mL of the bacterial liquid, dilute it by 10-fold gradient, and take 50 μL of the diluted bacterial liquid and spread it on an LB medium plate. 10 -5 , 10 -6 , 10 -7 Make three replicates for each dilution, incubate in a 37 °C incubator for 16 h, and observe the growth of Staphylococcus aureus colonies.
[0049] The results are as Figure 3 shown. The number of Staphylococcus aureus colonies growing on the LB medium plate after the mixed incubation of Lactobacillus plantarum 021, Staphylococcus aureus, and superabsorbent resin has no obvious difference from that of the control group of the co-incubation of Lactobacillus plantarum 021 and Staphylococcus aureus, and is significantly less than the number of colonies in the control group of the single incubation of Staphylococcus aureus, indicating that Lactobacillus plantarum 021 inhibits the growth of Staphylococcus aureus and its antibacterial activity is not affected by the superabsorbent resin.
[0050] Example 4: Lactobacillus plantarum 021 inhibits Escherichia coli and its antibacterial activity is not affected by superabsorbent resin
[0051] (1) Resuscitation and culture of Escherichia coli and Lactobacillus plantarum 021
[0052] Take out the bacterial strains stored in the -80°C refrigerator, and use an inoculation loop to streak on the appropriate solid medium (E. coli on LB medium, Lactobacillus plantarum 021 on MRS medium) by the method of three-zone streaking. After completion, place it upside down in an incubator and incubate for 48 h. In a laminar flow hood, use a pipette to aspirate 5 mL of the appropriate medium into a sterilized test tube, and then pick a single colony and inoculate it into the test tube with the appropriate medium, and place it on a shaker at 37°C for 12 h. Take the bacterial liquid from the previous step and inoculate it into a new medium at an inoculation amount of 2% for 1 subculture. Use the blank medium to adjust the bacterial liquid concentration to 1×10 9 CFU / mL.
[0053] (2) Preparation of superabsorbent resin
[0054] Weigh 0.1 g of superabsorbent resin, place it in a 50 mL centrifuge tube, add 25 mL of physiological saline, and fully swell it at room temperature for 12 h. Put the prepared sample into an autoclave and sterilize it at 121°C for 30 min for later use.
[0055] (3) Mixing and incubating superabsorbent resin with Lactobacillus plantarum 021 and Escherichia coli
[0056] Add 3 mL of MRS medium, 1 mL of Lactobacillus plantarum 021 bacterial liquid, and 1 mL of Escherichia coli bacterial liquid to the sterilized and cooled superabsorbent resin. After incubating on a shaker at 37°C for 12 h, take 1 mL of the bacterial liquid, dilute it by 10-fold gradient, and take 50 μL of the diluted bacterial liquid and spread it on an LB medium plate. 10 -5 , 10 -6 , 10 -7 Make three replicates for each dilution, incubate in a 37°C incubator for 16 h, and observe the growth of Escherichia coli colonies.
[0057] The results are as Figure 4 shown. The number of Escherichia coli colonies growing on the LB medium plate after the mixed incubation of Lactobacillus plantarum 021, Escherichia coli and superabsorbent resin has no obvious difference from the control group of the co-incubation of Lactobacillus plantarum 021 and Escherichia coli, and is significantly less than the number of colonies in the control group of Escherichia coli incubated alone, indicating that Lactobacillus plantarum 021 inhibits the growth of Escherichia coli and its antibacterial activity is not affected by the superabsorbent resin.
[0058] The results of Examples 1-4 show that Lactobacillus plantarum 021 has the activity of inhibiting pathogenic bacteria, and moreover, it maintains good growth activity and the activity of inhibiting pathogenic bacteria after mixing with superabsorbent resin. Lactobacillus plantarum 021 mixed with superabsorbent resin can inhibit the growth of harmful pathogenic bacteria in sanitary products such as sanitary napkins and prevent pathogenic bacteria from contaminating. Therefore, Lactobacillus plantarum 021 can be applied in the preparation of sanitary products such as sanitary napkins.
[0059] Although embodiments of the present invention are disclosed for illustrative purposes, those skilled in the art will understand that various substitutions, changes, and modifications are possible without departing from the spirit and scope of the present invention and the appended claims. Therefore, the scope of the present invention is not limited to the content disclosed in the embodiments.
Claims
1. A plant lactobacillus whose antibacterial activity is not affected by a water-absorbing resin, characterized in that: The name of the plant lactobacillus is: Lactobacillus plantarum 021, the classification name is: Lactobacillus plantarum, the preservation number is: CGMCC No.22169, the preservation date is: April 12, 2021, and the preservation unit is: General Microbiology Center of China Culture Collection Administration, Institute of Biology, Chinese Academy of Sciences, No. 3, No. 1 Beichen West Road, Chaoyang District, Beijing.
2. The plant lactobacillus whose antibacterial activity is not affected by the water-absorbing resin as claimed in claim 1, characterized in that: The plant lactobacillus has an inhibitory effect on the growth of Candida albicans, and after being mixed with the water-absorbing resin, the inhibitory effect on Candida albicans is not affected.
3. The plant lactobacillus whose antibacterial activity is not affected by the water-absorbing resin according to claim 1, characterized in that: The plant lactobacillus has an inhibitory effect on the growth of Staphylococcus aureus, and after being mixed with the water-absorbing resin, the inhibitory effect on Staphylococcus aureus is not affected.
4. The plant lactobacillus whose antibacterial activity is not affected by the water-absorbing resin according to claim 1, characterized in that: The plant lactobacillus has an inhibitory effect on the growth of Escherichia coli, and after being mixed with the water-absorbing resin, the inhibitory effect on Escherichia coli is not affected.
5. Use of the plant lactobacillus whose antibacterial activity is not affected by water-absorbing resin as claimed in any one of claims 1 to 4 mixed with water-absorbing resin in the preparation of sanitary napkins, tampons and other female hygiene products.
6. Use of the Lactobacillus plantarum whose antibacterial activity is not affected by the water-absorbing resin as claimed in any one of claims 1 to 5 mixed with a water-absorbing resin in the preparation of other sanitary products such as diapers.