Efficient culture device and culture method for fungus-carrying trichogramma

Abstract

The invention belongs to the technical field of trichogramma cultivation, and particularly relates to an efficient culture device and culture method for fungus-carrying trichogramma. The device comprises a box body, the box body is provided with a moving plate, a bee breeding plate for fixing bee bodies, a liquid box for containing liquid and conveying liquid or bacterial liquid, an intelligent control body for integrated control and other structures, and the effects of high bacterial carrying rate and high production efficiency are achieved.

Description

Technical Field

[0001] The present invention belongs to the technical field of Trichogramma breeding, and particularly relates to a high-efficiency culture device for bacterium-carrying Trichogramma and a culture method thereof. Background Art

[0002] Trichogramma is an important parasitic natural enemy insect and is widely used in the biological control of agricultural pests. It lays eggs inside the eggs of pests, making the host eggs unable to hatch, thereby achieving the purpose of controlling the pest population. Trichogramma has a significant control effect on Lepidoptera pests such as the Asian corn borer and the pine caterpillar, and has become one of the most widely used biological control means globally.

[0003] However, the control effect of traditional Trichogramma is limited by its single parasitic effect. It can only control the pest population by parasitizing the eggs of pests and cannot directly kill the larvae and adults of pests. In addition, the parasitic efficiency of Trichogramma is greatly affected by environmental factors such as temperature, humidity, and the quality of host eggs, resulting in unstable effects in practical applications.

[0004] To improve the control effect of Trichogramma, technicians have developed "bacterium-carrying Trichogramma". Bacterium-carrying Trichogramma refers to Trichogramma that is artificially made to carry pathogenic microorganisms such as insect viruses, Beauveria bassiana, and Bt bacteria. During the process of searching for host eggs, the pathogenic microorganisms are introduced into the pest population by Trichogramma, thereby achieving the dual control effect of "the wasp kills the eggs and the bacteria kill the larvae". This technology not only improves the control effect of Trichogramma but also significantly enhances its continuous control ability over the pest population. However, the existing technology is difficult to ensure that Trichogramma can carry pathogenic microorganisms efficiently and stably, resulting in unstable control effects. At the same time, the traditional culture method of bacterium-carrying Trichogramma relies on manual operation, with low efficiency and high cost, and it is difficult to meet the needs of large-scale production. Summary of the Invention

[0005] In view of the above technical problems, the present invention provides a high-efficiency culture device for bacterium-carrying Trichogramma with a high bacterium-carrying rate and high production efficiency and a culture method thereof.

[0006] Specifically, the first aspect of the present invention provides a high-efficiency culture device for bacterium-carrying Trichogramma,

[0007] a box body, the box body includes a main body with an opening on one side and a dirt box connected to the bottom of the box body;

[0008] wherein, the main body includes two side walls, a fixed wall connecting the two side walls, a screen bottom plate, and a top plate. A temperature regulator, evenly distributed adjustable nozzles, and a monitoring device arranged in the middle of the side wall are provided on the main body. A moving channel is provided at the end of the side wall, and a fixed cavity is provided on the fixed wall;

[0009] A moving plate, which moves through a moving channel and is opposite to a fixed wall;

[0010] A bee breeding plate, which includes a movable plate and a fixed bee body. The movable plate includes movable plate A and movable plate B. Movable plate A and movable plate B are connected by a buckle structure. Plate holes are evenly distributed on movable plate A and / or movable plate B. The fixed bee body includes a bee breeding area and a clamping area. The bee breeding plate can be movably inserted into a fixed cavity for fixation;

[0011] Among them, the bee breeding area is placed in the plate holes, and the clamping area is clamped and fixed by movable plate A and movable plate B;

[0012] A liquid tank, which is connected to an adjustable nozzle through a pipeline;

[0013] An intelligent control body, which can control the adjustable nozzle and the temperature regulator.

[0014] In some embodiments, after the bee breeding plate is inserted into the fixed cavity for fixation, the monitoring device can completely monitor the bee breeding plate.

[0015] In some embodiments, a suction fan and a waste bacterial liquid tank are further included, and the suction fan is controlled by the intelligent control body.

[0016] In some embodiments, the bee breeding area is loaded with 400 - 500 alternative host egg grains parasitized by Trichogramma evanescens per square centimeter.

[0017] In some embodiments, the intelligent control body includes an intelligent control system.

[0018] In some embodiments, the intelligent control system includes putting standard Trichogramma evanescens bee cards in different periods into the device, obtaining standard values through monitoring the standard Trichogramma evanescens bee cards by the monitoring device, recording them into the intelligent control system, and then during the development process of the bacterium-carrying Trichogramma evanescens, using the monitoring device for monitoring and comparing with the standard values to monitor the development of the bacterium-carrying Trichogramma evanescens.

[0019] In some embodiments, the standard values include the color of the bee eggs and the plumpness of the egg grains.

[0020] In some embodiments, the intelligent control system further includes monitoring and controlling the internal environment of the device.

[0021] The second aspect of the present invention provides a method for culturing bacterium-carrying Trichogramma evanescens, which is characterized in that the culturing method includes the following steps:

[0022] A. Load the Trichogramma evanescens bee eggs into the bee breeding area of the high-efficiency culturing device for bacterium-carrying Trichogramma evanescens, fix them with the movable plate, and put them into the device;

[0023] B. Add the bacterial solution into the liquid tank, control the adjustable nozzle to spray through the intelligent control body. At the same time, the intelligent control body detects and controls the concentration of the bacterial solution in the device. After spraying is completed, perform air drying through the exhaust fan to remove excess moisture and ensure that the bacterial solution is effectively adsorbed;

[0024] C. Cultivate until the pre-pupa stage, stop spraying the bacterial solution, and then the cultivation of Trichogramma carrying bacteria can be completed. Description of the Drawings

[0025] To more clearly illustrate the technical solutions in the embodiments of the present invention, the following will briefly introduce the drawings required for use in the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0026] Figure 1 is the structural diagram of the present invention;

[0027] Figure 2 is the structural diagram of the box body in the present invention;

[0028] Figure 3 is the schematic structural diagram of the bee breeding board in the present invention;

[0029] Figure 4 is the schematic structural diagram of the movable board in the present invention;

[0030] Figure 5 is the schematic structural diagram of the structure for fixing the bee body in the present invention. Detailed Embodiments

[0031] The following will clearly and completely describe the technical solutions of the present invention in conjunction with the drawings. Obviously, the described embodiments are some, but not all, of the embodiments of the present invention.

[0032] Generally, the components of the embodiments of the present invention described and shown in the drawings here can be arranged and designed in various different configurations. Therefore, the following detailed description of the embodiments of the present invention provided in the drawings is not intended to limit the scope of the claimed present invention, but merely represents the selected embodiments of the present invention.

[0033] Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts fall within the scope of protection of the present invention.

[0034] In the description of the present invention, it should be noted that unless otherwise clearly specified and defined, the terms "installation", "connection", and "coupling" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, and it can be the communication inside two components. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific situations.

[0035] The first aspect of the present invention provides an efficient cultivation device for Trichogramma bacteria-carrying wasps.

[0036] A box body, the box body includes a main body with an opening on one side and a dirt box connected to the bottom of the box body;

[0037] Among them, the main body includes two side walls, a fixed wall connecting the two side walls, a screen bottom plate, and a top plate. A temperature regulator, evenly distributed adjustable nozzles, and a monitoring device arranged in the middle of the side walls are provided on the main body. A moving channel is provided at the end of the side wall, and a fixed cavity is provided on the fixed wall;

[0038] A moving plate, the moving plate moves through the moving channel, and the moving plate is opposite to the fixed wall;

[0039] A wasp breeding plate, the wasp breeding plate includes a movable plate and a fixed wasp body. The movable plate includes movable plate A and movable plate B. Movable plate A and movable plate B are connected by a buckle structure. Plate holes are evenly distributed on movable plate A and / or movable plate B. The fixed wasp body includes a wasp breeding area and a clamping area. The wasp breeding plate can be movably inserted into the fixed cavity for fixation;

[0040] Among them, the wasp breeding area is placed in the plate holes, and the clamping area is clamped and fixed by movable plate A and movable plate B;

[0041] A liquid tank, the liquid tank is connected to the adjustable nozzles through a pipeline;

[0042] An intelligent control body, the intelligent control body can control the adjustable nozzles and the temperature regulator.

[0043] Through the above disclosure, the effects of high bacteria-carrying rate and high production efficiency can be obtained.

[0044] Next, an exemplary embodiment of the efficient cultivation device and cultivation method for Trichogramma bacteria-carrying wasps according to the present invention will be described with reference to the drawings.

[0045] At least one embodiment of the present disclosure provides an efficient cultivation device for Trichogramma bacteria-carrying wasps. Figure 1 The structural diagram of the efficient cultivation device for Trichogramma bacteria-carrying wasps shown. As Figure 1The shown box body 1, the box body 1 includes a main body 11 with an opening on one side and a dirt box 6 connected to the bottom of the box body 1;

[0046] Among them, the main body 11 includes two side walls 111, a fixed wall 112 connecting the two side walls 111, a screen bottom plate 16, and a top plate 113. A temperature regulator 13, evenly distributed adjustable nozzles 17, and a monitoring device 14 arranged in the middle of the side wall 111 are provided on the main body 11. Here, the monitoring device 14 can be a camera or an infrared imaging device, etc., which is a device that can be used to extract the characteristic parameters of bee eggs. A moving channel 18 is provided at the end of the side wall 111, and a fixed cavity 15 is provided on the fixed wall 112;

[0047] In the embodiment of the present disclosure, the temperature regulator 13 can be used to effectively adjust the temperature inside the device, ensure the normal breeding of bee eggs, and at the same time use the adjustable nozzles 17 to spray the bacterial liquid into the device, and can effectively adjust the bacterial concentration in the device, and monitor the survival rate of Trichogramma adults and the activity parameters of the bacterial liquid in real time, screen the critical concentration threshold that enables the survival rate of Trichogramma to be ≥90% and the maintenance rate of the activity of the bacterial liquid to be ≥80%, and stabilize the bacterial liquid concentration within ±5% of the critical concentration threshold through the feedback control system, ensure that the bee eggs gradually adapt to the presence of bacteria, effectively improve the coexistence with bacteria, increase the number of bacteria carried, and improve the cultivation method of bacteria-carrying Trichogramma.

[0048] A moving plate 12, the moving plate 12 moves through the moving channel 18, and the moving plate 12 is opposite to the fixed wall 112; the main body 11 can be effectively opened or closed by the moving method of the moving plate 12.

[0049] For example, Figure 3 shows a schematic structural diagram of a bee breeding plate, Figure 4 shows a schematic structural diagram of a movable plate, combined with Figure 2 and Figure 2 , a bee breeding plate 7, the bee breeding plate 7 includes a movable plate 71 and a fixed bee body 72. The movable plate 71 includes a movable plate A 711 and a movable plate B 712. The movable plate A 711 and the movable plate B 712 are connected through a buckle structure. For example, a clamping head is provided on the movable plate A 711, and a clamping groove is provided on the movable plate B 712. The two cooperate to achieve a good connection, and at the same time achieve the clamping effect on the fixed bee body 72. Plate holes 713 are evenly distributed on the movable plate A 711 and / or the movable plate B 712, as Figure 5 is a schematic structural diagram of the fixed bee body, combined with Figure 5 , the fixed bee body 72 includes a bee breeding area 721 and a clamping area 722. The bee breeding plate 7 can be movably inserted into the fixed cavity 15 for fixation. The material of the solid bee body 72 is paper, transparent plastic, etc. Bee eggs are fixed in the bee breeding area 721 with non-toxic glue.

[0050] For example, the bee breeding area 721 is placed in the plate hole 713, and the clamping area 722 is clamped and fixed by the movable plate A 711 and the movable plate B 712;

[0051] For example, as Figure 1 shown in the liquid tank 3, the liquid tank 3 is connected to the adjustable nozzle 17 through a pipeline; the liquid tank 3 can hold water or bacterial liquid, and bacterial liquids with different concentrations can be placed according to needs to meet the requirements of the bacterial liquid concentration in the device.

[0052] For example, in some embodiments, as Figure 1 shown in the intelligent control body 2, the intelligent control body 2 can control the adjustable nozzle 17 and the temperature regulator 13.

[0053] For example, in some embodiments, after the bee breeding plate 7 is inserted and fixed in the fixed cavity 15, the monitoring device 14 can fully monitor the bee breeding plate 7.

[0054] For example, in some embodiments, as Figure 1 shown in the exhaust fan 5 and the waste bacterial liquid tank 5, the exhaust fan 5 is controlled by the intelligent control body 2, and the exhaust fan 5 can be used to control the dryness inside the device to ensure the normal hatching of bee eggs.

[0055] Thus, as Figure 5 shown, the bee breeding area 721 is loaded with 400 - 500 alternative host egg grains parasitized by Trichogramma evanescens per square centimeter. Maintaining this density of loading can effectively improve the production effect of bacteria-carrying Trichogramma.

[0056] For example, in some embodiments, as Figure 1 shown, the intelligent control body 2 includes an intelligent control system. The intelligent control system includes putting standard Trichogramma cards at different times into the device, obtaining standard values through monitoring the standard Trichogramma cards by the monitoring device 14, and recording them into the intelligent control system. Subsequently, during the cultivation process of bacteria-carrying Trichogramma, the monitoring device 14 is used for monitoring and compared with the standard values to monitor the development of bacteria-carrying Trichogramma. If the comparison between the monitored value and the standard value is lower than the set minimum value, such as 80%, 85%, 90%, 95%, etc. (the minimum value is set according to actual needs), the intelligent control body 2 will give an alarm to remind the user to conduct inspections or adjustments.

[0057] Among them, the standard values include the color of bee eggs and the plumpness of egg grains.

[0058] For example, in some embodiments, the intelligent control system further includes the monitoring and control of the internal environment of the device.

[0059] The existence of the intelligent control body 2 in the present disclosure ensures the cultivation and self-help monitoring of bacterium-carrying Trichogramma, reduces production costs, and improves the cultivation effect at the same time.

[0060] At least one embodiment of the present disclosure provides a method for culturing bacterium-carrying Trichogramma, and the culturing method includes the following steps:

[0061] A. Load the Trichogramma eggs onto the bee-breeding area 721 in the high-efficiency culturing device for bacterium-carrying Trichogramma, fix them with the movable plate 71, and place them into the device;

[0062] B. Add the bacterial liquid into the liquid tank 3, control the adjustable nozzle 17 to spray through the intelligent control body 2, and at the same time, the intelligent control body 2 detects and controls the concentration of the bacterial liquid in the device. After spraying is completed, perform air-drying treatment through the exhaust fan 5 to remove excess moisture and ensure that the bacterial liquid is effectively adsorbed; during this process, the bacteria used are those that can cause the death or disease of pests, such as insect viruses, Beauveria bassiana, Bacillus thuringiensis, Metarhizium anisopliae, etc.;

[0063] C. Cultivate until the prepupa stage, stop spraying the bacterial liquid, and then the cultivation of bacterium-carrying Trichogramma can be completed.

[0064] In the above solution, the Trichogramma selected can be Trichogramma dendrolimi, Trichogramma ostriniae, Trichogramma chilonis, etc.

[0065] Through the above equipment and method, such as Beauveria bassiana and Trichogramma chilonis, the intelligent control body 2 controls the temperature at (26±1)°C, RH at (60±10)%, and the photoperiod at 14L:10D. The concentration of the Beauveria bassiana bacterial liquid is 10 4 spores / mL (spray three times at intervals of 3 hours) in the first stage, 10 5 spores / mL (spray three times at intervals of 3 hours) in the second stage, 10 6 spores / mL (spray three times at intervals of 3 hours) in the third stage, 10 7 spores / mL of the Beauveria bassiana spore suspension (spray once every 4 hours) in the fourth stage. The concentration of the bacterial liquid in the fourth stage is obtained through detection, and 10 7 spores / mL is detected as the critical concentration threshold and maintained. After cultivating until the prepupa stage, stop spraying the bacterial liquid. After the bee eggs emerge, detect the bacterium-carrying amount and the emergence rate. The bacterium-carrying amount is 4.26×10 4 spores / bee, and the emergence rate is above 91%.

[0066] The above is only the preferred specific implementation manner of the present invention, but the protection scope of the present invention is not limited thereto. Any person skilled in the art within the technical scope disclosed by the present invention, according to the technical solution and inventive concept of the present invention, makes equivalent substitutions or changes, and all should be covered within the protection scope of the present invention.

Claims

1. An efficient culture device for bacteria-carrying trichogrammatids, characterized in that: A box body (1), the box body (1) comprising a main body (11) with an opening on one side and a dirt box (6) connected to the bottom of the box body (1); The body (11) comprises two side walls (111), a fixed wall (112) connecting the two side walls (111), a screen bottom plate (16) and a top plate (113); the body (11) is provided with a temperature controller (13), evenly distributed adjustable nozzles (17) and a monitoring device (14) arranged in the middle of the side wall (111); a movable channel (18) is arranged at the end of the side wall (111); and a fixed cavity (15) is arranged on the fixed wall (112); A movable plate (12), the movable plate (12) moves through the movable channel (18), and the movable plate (12) is opposite to the fixed wall (112); A bee-raising plate (7), the bee-raising plate (7) comprising a movable plate (71) and a fixed bee body (72), the movable plate (71) comprising a movable plate A (711) and a movable plate B (712), the movable plate A (711) and the movable plate B (712) being connected via a snap-fit ​​structure, plate holes (713) being evenly distributed on the movable plate A (711) and / or the movable plate B (712), the fixed bee body (72) comprising a bee-raising area (721) and a clamping area (722), the bee-raising plate (7) being movably inserted into a fixed cavity (15) for fixing; The bee breeding area (721) is placed in the plate hole (713), and the clamping area (722) is clamped and fixed by the movable plate A (711) and the movable plate B (712); A liquid tank (3), wherein the liquid tank (3) is connected to the adjustable nozzle (17) via a pipeline; An intelligent control body (2), wherein the intelligent control body (2) can control an adjustable nozzle (17) and a temperature controller (13).

2. The efficient culture device for Trichogramma carrying bacteria according to claim 1, characterized in that: After the bee breeding board (7) is inserted into the fixing cavity (15) and fixed, the monitoring device (14) can completely monitor the bee breeding board (7).

3. The efficient culture device for bacteria-carrying trichogrammatids according to claim 2, characterized in that: It also includes an exhaust fan (5) and a waste bacteria liquid tank (5), wherein the exhaust fan (5) is controlled by the intelligent control body (2).

4. The efficient culture device for bacteria-carrying trichogrammatids according to claim 3, characterized in that: The bee breeding area (721) carries 400 to 500 parasitic host replacement eggs of Trichogramma per square centimeter.

5. The efficient culture device for bacteria-carrying trichogrammatids according to claim 4, characterized in that: The intelligent control body (2) comprises an intelligent control system.

6. The efficient culture device for bacteria-carrying trichogrammatids according to claim 5, characterized in that: The intelligent control system comprises placing standard trichogrammatid bee cards of different periods into a device, monitoring the standard trichogrammatid bee cards through a monitoring device (14) to obtain standard values, and recording them in the intelligent control system; subsequently, during the cultivation process of the bacteria-carrying trichogrammatids, the monitoring device (14) is used to monitor and compare the values ​​with the standard values ​​to monitor the development of the bacteria-carrying trichogrammatids.

7. The efficient culture device for Trichogramma carrying bacteria according to claim 6, characterized in that: The standard values ​​include the color of the bee eggs and the fullness of the eggs.

8. The efficient culture device for Trichogramma carrying bacteria according to claim 5, characterized in that: The intelligent control system also includes monitoring and controlling the internal environment of the device.

9. A method for cultivating a bacterium-carrying trichogrammatid, characterized in that: The culture method comprises the following steps: A. Loading the Trichogrammatid eggs in the bee breeding area (721) of the efficient culture device for bacteria-carrying Trichogrammatids according to any one of claims 1 to 8, fixing them by a movable plate (71), and placing them in the device; B. Add bacterial liquid into the liquid tank (3), and control the adjustable nozzle (17) to spray through the intelligent control body (2). At the same time, the intelligent control body (2) detects and controls the concentration of the bacterial liquid in the device. After spraying, the exhaust fan (5) is used for air drying to remove excess moisture and ensure that the bacterial liquid is effectively adsorbed; C. When the culture reaches the pre-pupae stage, stop spraying the bacterial solution and the culture of the fungus-carrying trichogrammatid is completed.

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