Bactericidal composition containing triazole compound and application thereof

Abstract

The present invention relates to a triazole compound-containing bactericidal composition and application thereof, the triazole compound-containing bactericidal composition comprises an active component A and an active component B, the active component A is a compound represented by formula (I), the active component B is a triazole compound, and the mass ratio of the active component A to the active component B is 1: 35-36: 1. The bactericidal composition has high antibacterial activity under a certain mass ratio, has an obvious synergistic effect on wheat scab, and has the effects of increasing yield and keeping harvest.

Description

Technical Field

[0001] The present invention relates to the field of pesticide fungicides, and specifically relates to a bactericidal composition containing a triazole compound and its application. Background Art

[0002] The compound of formula (I) is a methoxyacrylate fungicide developed by Sumitomo Chemical Co., Ltd., with CAS registration number: 2454319-63-0, and the Chinese chemical name: methyl (2Z)-3-methoxy-2-[(4-methyl[1,1'-biphenyl]-3-yl)oxy]prop-2-enoate, and its chemical structure is as follows:

[0003]

[0004] Gibberella zeae (Fusarium graminearum) infecting wheat causes Fusarium head blight, which is an important disease in current wheat production. However, due to the lack of stable high-yield disease-resistant wheat varieties in production, and chemical control has the advantages of being fast, efficient, and low-cost, so currently it is mainly controlled by chemical agents. Due to the long-term, large-scale, and unscientific application of fungicides to wheat, Gibberella zeae has developed varying degrees of resistance to a variety of fungicides including triazole fungicides.

[0005] The application of pesticides is an important means to prevent and control plant diseases. The present invention has conducted in-depth research on the application of the compound of formula (I) to Fusarium head blight. A large number of test results show that the compound of formula (I) is compounded with triazole fungicides, especially with propiconazole, cyflufenamid, penconazole, epoxiconazole, metconazole, etc., and can effectively improve the control effect at a certain mass ratio, with obvious synergistic effects, which is of great significance for ensuring the safe production of wheat. Summary of the Invention

[0006] Based on the above situation, the purpose of the present invention is to provide a bactericidal composition containing a triazole compound and its preparation, which is mainly used for preventing and controlling plant fungal diseases. This composition or its preparation can enhance the drug effect, reduce the dosage of the drug, and at the same time can extend the effective period and delay the development of drug resistance, and has high safety for crops.

[0007] This purpose is achieved through the following technical solutions: A bactericidal composition containing a triazole compound, comprising active ingredient A and active ingredient B, wherein the active ingredient A is the compound shown in formula (I), and the chemical structural formula: The active ingredient B is a triazole compound, and the triazole compound is selected from any one of propiconazole, cyflufenamid, penconazole, epoxiconazole, metconazole;

[0008] Furthermore, the mass ratio of the active ingredient A to the active ingredient B is 1:35 to 36:1;

[0009] Furthermore, the mass ratio of the active ingredient A to the active ingredient B is 1:32 to 30:1 or any value between the above values;

[0010] Furthermore, the mass ratio of the compound of formula (I) to propiconazole is 1:24 to 24:1;

[0011] Furthermore, the mass ratio of the compound of formula (I) to propiconazole is 1:24 to 16:1;

[0012] Still further, the mass ratio of the compound of formula (I) to propiconazole is 1:24, 1:16, 2:13, 5:2, 13:2, 16:1;

[0013] Furthermore, the mass ratio of the compound of formula (I) to difenoconazole is 1:20 to 30:1;

[0014] Furthermore, the mass ratio of the compound of formula (I) to difenoconazole is 1:20 to 20:1;

[0015] Still further, the mass ratio of the compound of formula (I) to difenoconazole is 1:20, 1:12, 1:6, 6:1, 12:1, 20:1;

[0016] Furthermore, the mass ratio of the compound of formula (I) to penconazole is 1:24 to 22:1;

[0017] Furthermore, the mass ratio of the compound of formula (I) to penconazole is 1:24 to 16:1;

[0018] Still further, the mass ratio of the compound of formula (I) to penconazole is 1:24, 1:8, 1:2, 2:1, 8:1, 16:1;

[0019] Furthermore, the mass ratio of the compound of formula (I) to epoxiconazole is 1:32 to 24:1;

[0020] Furthermore, the mass ratio of the compound of formula (I) to epoxiconazole is 1:18 to 14:1;

[0021] Still further, the mass ratio of the compound of formula (I) to epoxiconazole is 1:18, 1:7, 3:2, 7:1, 14:1;

[0022] Furthermore, the mass ratio of the compound of formula (I) to metconazole is 1:26 to 21:1;

[0023] Furthermore, the mass ratio of the compound of formula (I) to metconazole is 1:17 to 17:1;

[0024] Furthermore, the mass ratio of the compound of formula (I) to metconazole is 1:17, 1:7, 2:1, 7:1, 17:1;

[0025] Further, based on the total weight of the bactericidal composition being 100 wt%, the sum of the contents of active ingredient A and active ingredient B in the bactericidal composition is 1 - 90 wt%;

[0026] Further, based on the total weight of the bactericidal composition being 100 wt%, the sum of the contents of active ingredient A and active ingredient B in the bactericidal composition is preferably 5 - 80 wt%;

[0027] Further, the bactericidal composition further comprises agriculturally acceptable auxiliary ingredients in addition to the active ingredients, and the auxiliary ingredients are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreezing agents, defoaming agents, solvents, preservatives, stabilizers, synergists, binders or carriers;

[0028] Further, the wetting agent is selected from one or more of alkylbenzene sulfonates, alkylnaphthalene sulfonates, lignin sulfonates, sodium dodecyl sulfate, sodium dioctyl sulfosuccinate, α-olefin sulfonates, alkylphenol polyoxyethylene ethers, castor oil polyoxyethylene ethers, alkylphenol ethoxylates, fatty alcohol ethoxylates, sodium fatty alcohol polyoxyethylene ether sulfate, silkworm excrement, soapnut powder, sapindus powder, SOPA, detergent, emulsifier 2000 series and wetting penetrant F; and / or

[0029] Further, the dispersant is selected from one or more of lignin sulfonates, alkylnaphthalene sulfonate formaldehyde condensates, naphthalene sulfonates, triphenylvinylphenol ethoxylate phosphates, fatty alcohol ethoxylates, alkylphenol polyoxyethylene ethers, alkylphenol polyoxyethylene ether methyl ether condensate sulfates, fatty amine polyoxyethylene ethers, glycerol fatty acid ester polyoxyethylene ethers, polycarboxylates, polyacrylates, phosphates, EO-PO block copolymers and EO-PO graft copolymers; and / or

[0030] Further, the emulsifier is selected from one or more of calcium dodecylbenzenesulfonate, alkylphenol formaldehyde resin polyoxyethylene ether, phenethylphenol polyoxyethylene polyoxypropylene ether, fatty alcohol ethylene oxide-propylene oxide copolymer, styrylphenol polyoxyethylene ether, castor oil polyoxyethylene ether and alkylphenol ether phosphate; and / or

[0031] Further, the thickener is selected from one or more of xanthan gum, organobentonite, gum arabic, sodium alginate, magnesium aluminum silicate, carboxymethyl cellulose and silica white; and / or

[0032] Further, the disintegrant is selected from one or more of sodium sulfate, ammonium sulfate, aluminum chloride, sodium chloride, ammonium chloride, bentonite, glucose, sucrose, starch, cellulose, urea, sodium carbonate, sodium bicarbonate, citric acid, and tartaric acid; and / or

[0033] Further, the antifreeze is selected from one or more of alcohols, alcohol ethers, chlorinated hydrocarbons, and inorganic salts; and / or

[0034] Further, the defoamer is selected from C 10 -C 20 saturated fatty acid compounds, silicone oils, silicone compounds, C 8 -C 10 fatty alcohols; and / or

[0035] Further, the solvent is selected from one or more of benzene, toluene, xylene, durene, methanol, ethanol, isopropanol, n-butanol, dimethyl sulfoxide, dimethylformamide, cyclohexanone, alkylene carbonate, diesel oil, solvent naphtha, vegetable oils (such as soybean oil, corn oil, rapeseed oil, palm oil, etc.), vegetable oil derivatives, and water; and / or

[0036] Further, the preservative is selected from one or more of propionic acid, sodium propionate, sorbic acid, sodium sorbate, potassium sorbate, benzoic acid, sodium benzoate, sodium p-hydroxybenzoate, methyl p-hydroxybenzoate, Kathon, and 1,2-benzisothiazolin-3-one; and / or

[0037] Further, the stabilizer is selected from one or more of disodium hydrogen phosphate, oxalic acid, succinic acid, adipic acid, borax, 2,6-di-tert-butyl-p-cresol, triethanolamine oleate, epoxidized vegetable oil, kaolin, diatomaceous earth, bentonite, attapulgite, silica white, talcum powder, montmorillonite, and starch; and / or

[0038] Further, the synergist is selected from synergistic phosphorus and synergistic ether; and / or

[0039] Further, the carrier is selected from one or more of ammonium salts, ground natural minerals, ground artificial minerals, silicates, resins, waxes, solid fertilizers, water, organic solvents, mineral oils, vegetable oils, and vegetable oil derivatives.

[0040] Further, the dosage form of the bactericidal composition is selected from solid preparations and / or liquid preparations and / or seed treatment preparations;

[0041] The solid preparations include powders, granules, balls, tablets, strips, wettable powders, oil-dispersed powders, milk powders, water-dispersible granules, milk granules, water-dispersible tablets, soluble powders, soluble tablets, or soluble granules;

[0042] The liquid preparations described above include soluble solutions, sols, oils, film-forming oils, emulsifiable concentrates, latexes, dispersible liquids, ointments, emulsifiable concentrates, oil emulsions, microemulsions, fats, suspensions, microcapsule suspensions, oil suspensions, dispersible oil suspensions, suspension emulsions, microcapsule suspension-suspensions, microcapsule suspension-emulsifiable concentrates or microcapsule suspension-suspension emulsions;

[0043] Furthermore, the bactericidal composition described above can be prepared into a pesticide-acceptable preparation dosage form, and the preparation dosage form is a microemulsion, an emulsifiable concentrate, a suspension, a dispersible oil suspension, a soluble solution, an emulsifiable concentrate, a suspension emulsion, a microcapsule suspension, a water-dispersible granule, a wettable powder, a granule, a seed treatment suspension, a seed treatment dry powder;

[0044] Furthermore, the preparation dosage form is a microemulsion, an emulsifiable concentrate, a suspension, an emulsifiable concentrate, a water-dispersible granule, a seed treatment suspension.

[0045] The present invention also discloses the application of a bactericidal composition as described above in preventing and controlling plant diseases;

[0046] Furthermore, the plant diseases are plant diseases caused by fungi or bacteria;

[0047] Furthermore, the plant diseases are plant diseases caused by fungi;

[0048] Furthermore, the plant diseases caused by fungi are wheat head blight, wheat leaf rust, wheat powdery mildew. The beneficial effects of the present invention:

[0049] 1) The bactericidal composition of the present invention has a synergistic effect on the control effect under a certain mass ratio;

[0050] 2) The bactericidal composition of the present invention can reduce the dosage of each single agent and reduce the pesticide cost;

[0051] 3) The bactericidal composition and its preparation of the present invention are environmentally friendly and have high safety. Specific Embodiments

[0052] To make the technical solutions, objectives and advantages of the present invention clearer, the present invention is described with the following specific embodiments, but the present invention can be implemented in various forms and should not be limited by the embodiments described herein.

[0053] Indoor toxicity determination:

[0054] Indoor bioassay of wheat head blight

[0055] Test basis: NY / T 1156.2-2006 "Pesticide Bioassay Guidelines for Indoor Fungicides - Part 2: Petri Dish Method for Inhibiting the Mycelial Growth of Pathogenic Fungi"; NY / T 1156.6-2006 "Pesticide Bioassay Guidelines for Indoor Fungicides - Part 6: Determination of the Combined Action of Mixtures".

[0056] Tested pathogenic bacteria: Fusarium graminearum of wheat head blight;

[0057] Tested pesticides: propiconazole, cyflufenamid, penconazole, epoxiconazole, metconazole. All the above test pesticides were provided by the Group R & D Center.

[0058] Pesticide preparation: First, dissolve the tested technical material with acetone, and then dilute it with 0.1% Tween 80 aqueous solution to prepare single-agent mother liquors respectively, and set 5 series of mass concentrations according to the purpose of mixing and the activity of pesticides.

[0059] After melting the PDA medium with a microwave oven and cooling it to about 50°C, according to the principle from low concentration to high concentration, take 1 mL of the prepared test liquid to be measured and 9 mL of PDA medium and add them into a Petri dish with a diameter of 9 cm, mix well, and make drug-containing plates with corresponding concentrations.

[0060] Inoculation: For the cultivated pathogenic bacteria, under sterile conditions, use a punch with a diameter of 6 mm to punch out activated pathogenic fungal cakes. After the drug-containing medium solidifies, place the fungal cakes at the center position of the medium, and finally seal the Petri dish with sealing film, then place it in an incubator at 27°C with a relative humidity of 70% for cultivation. At the same time, set up a treatment without pesticides as a blank control, and repeat each treatment 3 times.

[0061] Investigation: According to the mycelial growth in the blank control Petri dish, investigate the mycelial growth of the pathogenic bacteria, and measure the colony diameter with a caliper, with the unit of millimeter (mm). Measure the diameter of each colony vertically once with the cross method and take the average value.

[0062] Calculation method: According to the investigation results, calculate the mycelial growth inhibition rate according to the following formula, with the unit of percentage (%), and retain two decimal places for the calculation results.

[0063] D = D 1 - D 2

[0064] In the formula:

[0065] D - The increased diameter of the colony;

[0066] D 1 - The diameter of the colony;

[0067] D 2 - The diameter of the fungal cake.

[0068]

[0069] I——Inhibition rate of hypha growth;

[0070] D 0 ——Colony growth diameter of blank control;

[0071] D t ——Colony growth diameter of medicament treatment.

[0072] Test statistics: The data was processed by the method of probit analysis. Analyzed by DPS statistical analysis system to obtain the virulence regression line and EC 50 value, and evaluate the activity of the test medicament on the biological test materials.

[0073] Sun Yunpei method: Evaluate the synergistic effect of medicament mixture according to the co-toxicity coefficient (CTC). When the co-toxicity coefficient CTC of the compound is ≥120, it shows a synergistic effect; when CTC ≤80, it shows an antagonistic effect; when 80 < CTC < 120, it shows an additive effect.

[0074] The co-toxicity coefficient (CTC value) of the mixture is calculated by the following formula:

[0075]

[0076] Where:

[0077] ATI——Actual toxicity index of the mixture;

[0078] S——EC of the standard fungicide 50 , in milligrams per liter (mg / L);

[0079] M——EC of the mixture 50 , in milligrams per liter (mg / L).

[0080] TTI = TI A *P A +TI B *P B

[0081] Where:

[0082] TTI——Theoretical toxicity index of the mixture;

[0083] TI A ——Toxicity index of agent A;

[0084] P A ——Percentage content of agent A in the mixture, in percentage (%);

[0085] TI B ——Toxicity index of agent B;

[0086] P B —— Percentage content of agent B in the mixture, unit: percentage (%)

[0087]

[0088] In the formula:

[0089] CTC - Co-toxicity coefficient

[0090] ATI - Measured toxicity index of the mixture

[0091] TTI - Theoretical toxicity index of the mixture

[0092] Test results:

[0093] Table 1 results show that the EC of propiconazole against Gibberella zeae of wheat 50 is 0.201 mg / L. (I) The compound shows additive or synergistic effects when mixed with propiconazole in the mass ratio range of 1:35 to 24:1. Among them, when the mass ratio is 1:24 to 24:1, the co-toxicity coefficient > 120, showing a synergistic effect. The co-toxicity coefficient value of the compound of formula (I) and propiconazole = 5:2 is the largest, which is 246.068, and the EC 50 is 0.188 mg / L, with a significant synergistic effect.

[0094] Indoor determination results of different ratios of the compound of formula (Ⅰ) and propiconazole against Gibberella zeae of wheat in Table 1

[0095]

[0096] Table 2 results show that the EC of cyflufenamid against Gibberella zeae of wheat 50 is 0.406 mg / L. (I) The compound shows a synergistic effect when mixed with cyflufenamid in the mass ratio range of 1:20 to 30:1. Among them, when the mass ratio is 1:20 to 20:1, the co-toxicity coefficient > 130, with a significant synergistic effect. The co-toxicity coefficient value of the compound of formula (I) and cyflufenamid = 1:6 is the largest, which is 187.529, and the EC 50 is 0.236 mg / L, with a significant synergistic effect.

[0097] Indoor determination results of different ratios of the compound of formula (Ⅰ) and cyflufenamid against Gibberella zeae of wheat in Table 2

[0098]

[0099]

[0100] Table 3 results show that the EC of penconazole against Gibberella zeae of wheat 50It is 0.758 mg / L. (I) The compound shows additive or synergistic effects within the mass ratio range of 1:33 to 22:1 when mixed with penconazole. Among them, when the mass ratio is 1:24 to 22:1, the co-toxicity coefficient > 120, showing a synergistic effect. The co-toxicity coefficient value of the compound of formula (I) and penconazole = 1:2 is the largest, which is 222.053, and the EC 50 is 0.367 mg / L, and the synergistic effect is significant.

[0101] Table 3 Indoor determination results of different ratios of the compound of formula (Ⅰ) and penconazole against Gibberella zeae

[0102]

[0103]

[0104] Table 4 The results show that the EC of epoxiconazole against Gibberella zeae 50 is 0.394 mg / L. (I) The compound shows additive or synergistic effects within the mass ratio range of 1:32 to 36:1 when mixed with epoxiconazole. Among them, when the mass ratio is 1:32 to 24:1, the co-toxicity coefficient > 120, showing a synergistic effect. The co-toxicity coefficient value of the compound of formula (I) and epoxiconazole = 3:2 is the largest, which is 183.270, and the EC 50 is 0.332 mg / L, and the synergistic effect is significant.

[0105] Table 4 Indoor determination results of different ratios of the compound of formula (Ⅰ) and epoxiconazole against Gibberella zeae

[0106]

[0107] Table 5 The results show that the EC of metconazole against Gibberella zeae 50 is 0.195 mg / L. (I) The compound shows a synergistic effect within the mass ratio range of 1:26 to 21:1 when mixed with metconazole. Among them, when the mass ratio is 1:17 to 17:1, the co-toxicity coefficient > 150, and the synergistic effect is significant. The co-toxicity coefficient value of the compound of formula (I) and metconazole = 2:1 is the largest, which is 221.282, and the EC 50 is 0.189 mg / L, and the synergistic effect is significant.

[0108] Table 5 Indoor determination results of different ratios of the compound of formula (Ⅰ) and metconazole against Gibberella zeae

[0109]

[0110] Formulation examples

[0111] Preparation example 1: 40% compound of formula (I) · propiconazole emulsifiable concentrate (29:11)

[0112] Formulation: 29% of the compound of formula (I), 11% of propiconazole, 1.2% of BHT, 10% of propylene carbonate, 8% of DMF, 5% of dimethyl sulfoxide, 4% of cyclohexanone, 3% of calcium dodecylbenzenesulfonate, 12% of fatty alcohol polyoxyethylene ether, and the balance made up with methyl oleate;

[0113] Preparation method: Add the active ingredients to the cosolvent according to the formulation ratio of the example, and add surfactants and other functional auxiliaries thereto, and stir and mix evenly in a stirring and mixing kettle to obtain an emulsifiable concentrate.

[0114] Preparation Example 2: 30% compound of formula (I) · tebuconazole aqueous emulsion (10:20)

[0115] Formulation: 10% of the compound of formula (I), 20% of tebuconazole, 12% of pseudocumene, 10% of cyclohexanone, 1% of calcium dodecylbenzenesulfonate, 0.5% of sodium lauryl polyoxyethylene ether sulfate, 5% of EO / PO block copolymer, 5% of glycerol, 1.5% of glycerin, 0.1% of silicone defoamer, 0.15% of xanthan gum, 0.1% of sodium benzoate, and the balance made up with deionized water;

[0116] Preparation method: According to the formulation ratio of the example, dissolve the active ingredients in the solvent and add emulsifiers to form a homogeneous oil phase. Mix deionized water, antifreeze agents, and auxiliaries together to form a homogeneous aqueous phase. Under high-speed shearing, add the aqueous phase to the oil phase to form a well-dispersed aqueous emulsion preparation.

[0117] Preparation Example 3: 26% compound of formula (I) · propiconazole aqueous emulsion (16:10)

[0118] Preparation formulation: 16% of the compound of formula (I), 10% of propiconazole, 12% of pseudocumene, 10% of cyclohexanone, 1% of calcium dodecylbenzenesulfonate, 0.5% of sodium lauryl polyoxyethylene ether sulfate, 5% of EO / PO block copolymer, 5% of glycerol, 2% of glycerin, 0.1% of silicone defoamer, 0.2% of xanthan gum, 0.1% of sodium benzoate, and the balance made up with deionized water;

[0119] Preparation method: The same as Preparation Example 2.

[0120] Preparation Example 4: 24% compound of formula (I) · cyflufenamid emulsifiable concentrate (4:20)

[0121] Preparation formulation: 4% of the compound of formula (I), 20% of cyflufenamid, 1.5% of BHT, 10% of propylene carbonate, 15% of DMF, 5% of decanamide, 3.5% of calcium dodecylbenzenesulfonate, 10% of fatty alcohol polyoxyethylene ether, and the balance made up with methyl oleate;

[0122] Preparation method: The same as Preparation Example 1.

[0123] Preparation Example 5: 42% Compound of Formula (I) · Cyflufenamid Suspension Concentrate (6:36)

[0124] Formulation: 6% of the compound of formula (I), 36% of cyflufenamid, 4% of polyether, 3% of phenethylphenol polyether phosphate salt, 3% of dioctyl sulfosuccinate sodium salt, 1% of sodium polycarboxylate, 0.5% of magnesium aluminum silicate, 0.2% of xanthan gum, 5% of glycerol, 0.15% of methylisothiazolinone, 0.5% of silicone defoamer, deionized water to make up the balance;

[0125] Preparation method: According to the formula ratio, the active ingredient, surfactant and other functional auxiliaries are placed in the reaction kettle in turn, mixed evenly with water, subjected to high-speed shearing and wet grinding, and finally homogenized and filtered to obtain the suspension concentrate.

[0126] Preparation Example 6: 25% Compound of Formula (I) · Penconazole Suspension Concentrate (12:13)

[0127] Formulation: 12% of the compound of formula (I), 13% of penconazole, 4% of polyether, 3% of phenethylphenol polyether phosphate salt, 3% of naphthalene sulfonate formaldehyde condensate, 1% of magnesium aluminum silicate, 0.2% of xanthan gum, 0.5% of fumed silica, 0.5% of polyvinylpyrrolidone, 5% of ethylene glycol, 0.15% of methylisothiazolinone, 0.5% of silicone oil, deionized water to make up the balance;

[0128] Preparation method: The same as Preparation Example 5.

[0129] Preparation Example 7: 30% Compound of Formula (I) · Epoxiconazole Suspension Concentrate (18:12)

[0130] Formulation: 18% of the compound of formula (I), 12% of epoxiconazole, 5% of polyether, 3% of dioctyl sulfosuccinate sodium salt, 3% of styrylphenol polyoxyethylene ether phosphate salt, 1% of sodium polycarboxylate, 1% of magnesium aluminum silicate, 0.25% of xanthan gum, 5% of glycerol, 0.15% of methylisothiazolinone, 0.5% of silicone defoamer, deionized water to make up the balance;

[0131] Preparation method: The same as Preparation Example 5.

[0132] Preparation Example 8: 18% Compound of Formula (I) · Metconazole Suspension Concentrate (12:6)

[0133] Formulation: 12% of the compound of formula (I), 6% of metconazole, 4% of polyether, 3% of phenethylphenol polyether phosphate salt, 3% of dioctyl sulfosuccinate sodium salt, 1.5% of sodium polycarboxylate, 1% of magnesium aluminum silicate, 0.3% of xanthan gum, 5% of ethylene glycol, 0.15% of methylisothiazolinone, 0.5% of silicone oil, deionized water to make up the balance;

[0134] Preparation method: The same as Preparation Example 5.

[0135] Preparation Example 9: 60% Compound of Formula (I) · Epoxiconazole Water Dispersible Granules (36:24)

[0136] Formulation: 36% of the compound of formula (I), 24% of epoxiconazole, 3% of sodium dodecyl sulfate, 8% of naphthalene sulfonate formaldehyde condensate, 1% of sodium lauryl polyoxyethylene ether sulfate, 2% of sodium polycarboxylate, 6.5% of sodium lignosulfonate, 5% of attapulgite, and the balance made up with kaolin;

[0137] Preparation method: According to the formulation ratio, add the active ingredient to the carrier, add surfactants and other functional auxiliaries thereto, mix, add 10 - 25% of water after air flow pulverization, and then obtain the water dispersible granule product through kneading, granulation, drying, and screening; or spray water, granulate, and dry the pulverized powder in a fluidized bed granulator, and then screen to obtain the product.

[0138] Preparation Example 10: 45% Compound of Formula (I) · Metconazole Water Dispersible Granules (35:10)

[0139] Formulation: 35% of the compound of formula (I), 10% of metconazole, 3% of sodium dodecyl sulfate, 8% of naphthalene sulfonate formaldehyde condensate, 1% of sodium lauryl polyoxyethylene ether sulfate, 2% of sodium polycarboxylate, 5.5% of sodium lignosulfonate, 5% of attapulgite, and the balance made up with kaolin;

[0140] Preparation method: The same as Preparation Example 9.

[0141] Field Efficacy Test

[0142] Field Efficacy Test of Wheat Scab

[0143] Test basis: Refer to the Industry Standard of the People's Republic of China "Pesticide Field Efficacy Test Guidelines Part 15: Fungicides for Controlling Wheat Scab (NY / T 1464.15 - 2007)";

[0144] Test site: Wheat field in Weifang City, Shandong Province;

[0145] Test target: Wheat scab;

[0146] Test crop and variety: Wheat (Zhengmai 9023);

[0147] Test setup: Each plot is distributed according to a randomized block design, and a protection row is set around each plot. Each treatment has 4 replicates, and each plot is 20m 2 , and the plots are randomly arranged.

[0148] Application period: The first application is at the initial flowering stage (April 20th); the second application is made after a 7 - day interval, with a total of 2 applications, and the water consumption is 450L / hm 2 .

[0149] Test drug:

[0150] Table 6 Experimental treatment

[0151]

[0152]

[0153] Application method: Use conventional spray method and spray evenly on the front and back of the leaves.

[0154] Survey and statistical methods: Survey was conducted 10 days after the last application of medicine. Five diagonal points were randomly selected for sampling in each plot, 20 plants were surveyed at each point, and the top three leaves of each plant were surveyed (including the flag leaf if there was one), and the lesions on each leaf were graded according to the percentage of the entire leaf area.

[0155] Grading method:

[0156] Level 0: no disease;

[0157] Level 1: The lesion area accounts for less than 5% of the entire leaf area;

[0158] Level 3: The lesion area accounts for 6% to 25% of the entire leaf area;

[0159] Level 5: The lesion area accounts for 26% to 50% of the entire leaf area;

[0160] Level 7: The lesion area accounts for 51% to 75% of the entire leaf area;

[0161] Level 9: The lesion area accounts for more than 76% of the total leaf area.

[0162] Calculation method of drug efficacy

[0163] The disease index and control efficacy were calculated using the following formula

[0164]

[0165] During the experiment, wheat in each treatment plot grew well and no pesticide damage was observed in any treatment.

[0166] The test results are shown in the following table:

[0167] Table 7 Field efficacy test results of each treatment mixture for controlling wheat scab

[0168]

[0169]

[0170] As can be seen from the above Table 7 of the field efficacy trials, the compounds shown in (I) and propiconazole, cyflufenamid, penconazole, epoxiconazole, and metconazole showed good control effects against Fusarium head blight of wheat. 10 days after the last application, the control effects of 26% compound (I)·propiconazole aqueous emulsion (16:10), 24% compound (I)·cyflufenamid emulsifiable concentrate (4:20), 30% compound (I)·penconazole aqueous emulsion (10:20), 30% compound (I)·epoxiconazole suspension concentrate (18:12), and 18% compound (I)·metconazole suspension concentrate (12:6) against Fusarium head blight of wheat were 83.16%, 82.27%, 82.64%, 82.62%, and 82.10% respectively, all showing good control effects.

[0171] During the test process, no phytotoxicity and no impact on crop growth and development were recorded, indicating that the test agents were safe for crops within the recommended application rates. No impact on non-target organisms was found for the agents within the dose range. In addition, no extreme weather conditions such as heavy rainfall and strong winds were recorded during the test period.

[0172] Through indoor toxicity determination and field trials, the composition obtained by compounding the compound shown in formula (I) of the present invention with propiconazole, cyflufenamid, penconazole, epoxiconazole, and metconazole showed very good control effects against Fusarium head blight of wheat leaves. The bactericidal composition or its preparation obtained by compounding in the present invention had significant control effects and had the characteristics of high efficiency, broad spectrum, low residue, long-lasting efficacy, and strong systemic absorption; and no phytotoxicity was found for the compounded agents to crops during the test, indicating that under the condition of improved bactericidal synergistic effect of the obtained bactericidal composition or preparation, the production cost and use cost could be reduced and it was safe for crops.

[0173] Although the present invention has been described in detail with general descriptions and specific embodiments above, based on the present invention, some modifications or improvements can be made, which are obvious to those skilled in the art. Therefore, these modifications or improvements made without departing from the spirit of the present invention all fall within the scope of protection required by the present invention.

Claims

1. A bactericidal composition containing a triazole compound, characterized in that: It contains active ingredient A and active ingredient B, wherein the active ingredient A is a compound represented by formula (I), and the chemical structure is: The active ingredient B is a triazole compound, and the triazole compound is selected from any one of propiconazole, clofoconazole, penconazole, fluopicolide and metconazole.

2. The bactericidal composition according to claim 1, characterized in that The mass ratio of the active ingredient A to the active ingredient B is 1:35 to 36:1; Preferably, the mass ratio of the active ingredient A to the active ingredient B is 1:32 to 30:

1.

3. The bactericidal composition according to claim 1, characterized in that The mass ratio of the compound of formula (I) to propiconazole is 1:24 to 24:

1. The mass ratio of the compound of formula (I) to clofoconazole is 1:20 to 30:

1. The mass ratio of the compound of formula (I) to penconazole is 1:24 to 22:

1. The mass ratio of the compound of formula (I) to epoxiconazole is 1:32 to 24:

1. The mass ratio of the compound of formula (I) to metconazole is 1:26 to 21:1; Preferably, the mass ratio of the compound of formula (I) to propiconazole is 1:24 to 16:

1. The mass ratio of the compound of formula (I) to clofoconazole is 1:20 to 20:

1. The mass ratio of the compound of formula (I) to penconazole is 1:24 to 16:

1. The mass ratio of the compound of formula (I) to epoxiconazole is 1:18 to 14:

1. The mass ratio of the compound of formula (I) to metconazole is 1:17 to 17:

1.

4. The bactericidal composition according to claim 1, characterized in that Based on the total weight of the bactericidal composition being 100 wt %, the sum of the contents of the active ingredient A and the active ingredient B in the bactericidal composition is 1 to 90 wt %, preferably 5 to 80 wt %.

5. The bactericidal composition according to claim 1, characterized in that In addition to the active ingredients, the fungicide composition also contains agriculturally permitted auxiliary ingredients, and the auxiliary ingredients are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoaming agents, solvents, preservatives, stabilizers, synergists, binders or carriers.

6. The bactericidal composition according to claim 5, characterized in that The dosage form of the bactericidal composition is selected from solid preparations and / or liquid preparations.

7. The bactericidal composition according to claim 6, characterized in that The bactericidal composition can be prepared into a formulation acceptable to pesticides, and the formulation is a microemulsion, emulsifiable concentrate, suspension, aqueous emulsion, water-dispersible granules, and seed treatment suspension.

8. Use of the fungicidal composition according to any one of claims 1 to 7 in preventing and controlling plant diseases.

9. The use according to claim 8, characterized in that: The plant disease is a plant disease caused by fungi or bacteria; Preferably, the plant disease is a plant disease caused by fungi.

10. The use according to claim 9, characterized in that: The plant disease caused by the fungus is wheat fusarium rust.

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