Laying hen feed additive capable of reducing egg cholesterol and application of laying hen feed additive

Abstract

The invention relates to the technical field of poultry breeding, in particular to a laying hen feed additive capable of reducing egg cholesterol and application of the laying hen feed additive. The additive is prepared from 5%-15% of rumex hanus powder and the balance of conventional laying hen daily ration meeting the NY / T 33-2004 standard. By continuously feeding the laying hens for at least four weeks in the laying period, the cholesterol content of the eggs can be remarkably reduced, the laying rate is maintained to be greater than or equal to 90% under the condition of 10% of the additive amount, and the color of yolk is not remarkably changed. Flavonoid compounds in rumex hanus play a role in promoting bile acid excretion and cholesterol transport by inhibiting liver cholesterol synthetase. The method is low in cost, easy and convenient to operate and suitable for large-scale breeding, and a green and efficient solution is provided for low-cholesterol egg production.

Description

Technical Field

[0001] The present invention relates to the technical field of poultry farming, and specifically relates to a laying hen feed additive for reducing egg cholesterol and its application. Background Art

[0002] 1. Problems in the prior art

[0003] As a source of high-quality protein, eggs have a relatively high cholesterol content (about 580 mg / 100 g). Long-term excessive intake may pose potential risks to human cardiovascular health. The existing technical means for reducing egg cholesterol mainly include adjusting the diet formula of laying hens, such as adding functional ingredients such as flaxseed, fish oil, and phytosterols. However, they generally have defects such as high cost, complex operation, sensitive addition ratio, or unstable effects, and it is difficult to meet the economic and stable requirements of large-scale farming.

[0004] 2. Research status of Rumex patientia in animal feed

[0005] Rumex patientia L., also known as protein grass, is a perennial herbaceous plant rich in protein (≥30%), dietary fiber, vitamins, and various active ingredients (such as flavonoids, saponins, polyphenols, etc.). Modern pharmacological research shows that its active ingredients have the effects of regulating lipid metabolism and inhibiting hepatic cholesterol synthesis, and have potential application value in human health foods and livestock and poultry feeds. However, as of the filing date of this application, there has been no public report on the application of Rumex patientia in laying hen feed to reduce egg cholesterol, and its application effect and mechanism in laying hen farming are still blank.

[0006] Based on the deficiencies of the prior art, the present invention aims to provide a laying hen feed additive for reducing egg cholesterol and a feeding method that are low-cost, easy to operate, and have significant effects to fill the market gap. Summary of the Invention

[0007] In view of the problems mentioned in the above background, the present invention provides a laying hen feed additive for reducing egg cholesterol and its application.

[0008] A laying hen feed additive for reducing egg cholesterol and its application, comprising 5%-15% of Rumex patientia powder, and the balance being a conventional laying hen diet;

[0009] The Rumex patientia powder is made by drying and pulverizing the stems and leaves of Rumex patientia to 20 mesh.

[0010] Preferably, the conventional laying hen diet complies with NY / T 33-2004 "Feeding Standards for Chickens" and includes corn, soybean meal, limestone, monocalcium phosphate, vitamin and mineral premixes.

[0011] A method for applying a feed additive for laying hens is to continuously feed the feed additive to laying hens for at least 4 weeks, with a daily feeding amount of 100 - 120 g / hen, and the feeding stage is the laying period of laying hens at 20 - 60 weeks of age.

[0012] Preferably, the preferred addition amount of the Portulaca oleracea powder is 10%, at this time the cholesterol content in eggs is reduced by 58 - 60%, and the egg production rate ≥ 90%.

[0013] The beneficial effects of the present invention are as follows:

[0014] High - efficiency lipid - lowering: Adding 5% - 15% Portulaca oleracea powder can significantly reduce the cholesterol in eggs, and the effect is better than that of traditional additives.

[0015] Stable performance: Maintaining the egg production rate ≥ 90%, with no significant change in the yolk color, ensuring the breeding benefits.

[0016] Low cost: The raw material of Portulaca oleracea is easily available and the processing is simple, and the cost is much lower than traditional components such as fish oil.

[0017] Safe and easy to use: It is a natural plant component, without toxic residues, and is suitable for large - scale breeding processes.

[0018] Technological innovation: For the first time, Portulaca oleracea is used for lipid - lowering in laying hens, filling the gap in the industry.

[0019] The present invention realizes the reduction of egg cholesterol through natural plant components, and has high efficiency, economy and safety. It provides a scientific and feasible technical path for the production of "healthy eggs", and is of great significance for improving the added value of egg products and meeting the health needs of consumers. The feed additive of the present invention can effectively reduce egg cholesterol by regulating the lipid metabolism of laying hens, and has no significant negative impact on production performance, and is suitable for large - scale application. Specific embodiments

[0020] The following will describe the implementation plan of the present invention in detail in combination with embodiments. However, those skilled in the art will understand that the following embodiments are only used to illustrate the present invention and are not regarded as limiting the scope of the present invention. For those not specified in the embodiments regarding specific technologies or conditions, they shall be carried out according to the technologies or conditions described in the literature in the field or according to the product instructions.

[0021] A feed additive for laying hens to reduce egg cholesterol

[0022] 1. Composition of the feed additive

[0023] The feed additive is based on a conventional laying hen diet and contains 5%-15% of Portulaca oleracea powder by mass percentage, with the balance being the components of the conventional laying hen diet. Among them, the conventional laying hen diet includes, but is not limited to, conventional components such as corn, soybean meal, limestone powder, calcium hydrogen phosphate, table salt, vitamin and mineral premix, etc., and its composition complies with NY / T 33-2004 "Feeding Standards for Chickens".

[0024] The preparation method of Portulaca oleracea powder is as follows: Harvest the tender stems and leaves of Portulaca oleracea, dry them naturally or by hot air drying at 60-80°C until the moisture content ≤ 10%, crush them to 20 meshes, and store them sealed for later use after sieving. Controlling the crushing particle size can ensure uniform mixing of Portulaca oleracea powder and the basal diet, and avoid uneven feeding or affecting palatability caused by too large particles.

[0025] 2. Feeding method

[0026] Applicable stage: Laying period of laying hens, preferably laying hens at the peak laying period aged 20-60 weeks. Laying hens in this stage are sensitive to nutritional requirements, have a strong cholesterol deposition ability, and the intervention effect is more significant.

[0027] Feeding cycle: Continuously feed for at least 4 weeks, and it is recommended to feed for 8-12 weeks to maintain continuous effect. Experimental data show that a significant decrease in cholesterol can be observed after feeding for 4 weeks. Prolonging the feeding cycle can further consolidate the effect and there is no cumulative toxicity.

[0028] Feeding method: Mix Portulaca oleracea powder and the basal diet evenly in proportion, and feed regularly every day. The daily feeding amount is 100-120 g / head, and it can be adjusted slightly according to factors such as the body weight, laying rate and season of laying hens. The preparation of the mixed feed should follow GB / T31517-2015 "General Requirements for the Safety of Feed Raw Materials" to ensure hygiene and safety.

[0029] 3. Mechanism of action

[0030] Flavonoid compounds (such as rutin, quercetin) in Portulaca oleracea can reduce egg cholesterol through the following ways: ① Inhibit the activity of liver HMG-CoA reductase and reduce endogenous cholesterol synthesis; ② Promote bile acid excretion and accelerate cholesterol metabolism; ③ Enhance the activity of lecithin-cholesterol acyltransferase (LCAT), promote the transport of cholesterol to high-density lipoproteins, and reduce deposition in egg yolk. In addition, dietary fiber can adsorb intestinal cholesterol, reduce its absorption rate, and play a synergistic role in reducing lipid levels.

[0031] The experimental methods and data are as follows

[0032] I. Experimental materials and methods

[0033] 1. Experimental animals

[0034] Three hundred healthy 24-week-old Hailan Brown laying hens were selected and randomly divided into 4 groups, with 75 hens in each group. There were no significant differences in the initial egg production rate and average body weight among the groups (P > 0.05).

[0035] 2. Experimental grouping

[0036] Table 1

[0037] Group Addition amount of Rumex patientia powder (%) Composition of basal diet Feeding period Control group 0 Conventional diet 8 weeks Experimental group 1 5 Conventional diet + 5% Rumex patientia powder 8 weeks Experimental group 2 10 Conventional diet + 10% Rumex patientia powder 8 weeks Experimental group 3 15 Conventional diet + 15% Rumex patientia powder 8 weeks

[0038] 3. Detection indexes

[0039] Egg cholesterol content: Eggs of each group were collected weekly, and the cholesterol content of egg yolks was measured with reference to GB 5009.128-2016 National Food Safety Standard - Determination of Cholesterol in Foods, and the average value was taken.

[0040] Egg production rate: The number of eggs produced was recorded daily, and the weekly egg production rate of each group was calculated.

[0041] Egg yolk color: The egg yolk color was evaluated using a Roche color fan (1-15 points), and 20 eggs were randomly detected weekly.

[0042] II. Experimental results

[0043] 1. Changes in cholesterol content

[0044] Table 2

[0045] Group Initial cholesterol (mg / 100g) Cholesterol after 8 weeks (mg / 100g) Reduction rate (%) Control group 585±10 578±12 - Experimental group 1 585±10 250±8 57.2 Experimental group 2 585±10 235±7 59.8 Experimental group 3 585±10 230±6 60.7

[0046] 2. Egg production rate and egg yolk color

[0047] Table 3

[0048] Group Average egg production rate (%) Yolk color score (Roche color fan) Control group 92±1 8.5±0.3 Experimental group 1 91±2 8.3±0.5 Experimental group 2 90±1 8.2±0.4 Experimental group 3 88±2 8.0±0.6

[0049] III. Conclusion

[0050] Verified by animal experiments, the feed additive of the present invention can significantly reduce the cholesterol content of eggs, and the specific effects are as follows:

[0051] When the addition amount of Portulaca oleracea powder is 5%, the cholesterol content of eggs is reduced by 57.2%;

[0052] When the addition amount is 10%, the cholesterol content of eggs is reduced by 59.8%;

[0053] When the addition amount is 15%, the cholesterol content of eggs is reduced by 60.7%, but the egg production rate decreases slightly (≤5%).

[0054] At the preferred addition amount of 10%, the egg yolk color score (Roche color fan) is maintained at 8-9 points, there is no significant difference from the control group, and the egg production rate remains ≥90%, achieving a balance between lipid-lowering effect and production performance.

[0055] The experimental results show that the feed additive of the present invention can effectively reduce the cholesterol in eggs by regulating the lipid metabolism of laying hens, and has no significant negative impact on production performance, making it suitable for large-scale application.

[0056] The above are only the preferred embodiments of the present invention patent, and are not intended to limit the present invention patent. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention patent shall be included within the protection scope of the present invention patent.

Claims

1. A feed additive for laying hens for reducing egg cholesterol, characterized in that: Contains 5%-15% leafy grass meal, the remainder is regular laying hen diet; The leaf-eating grass powder is prepared by drying the stems and leaves of the leaf-eating grass and crushing them into 20 meshes.

2. The feed additive for laying hens for reducing egg cholesterol according to claim 1, characterized in that: The conventional laying hen diet complies with NY / T 33-2004 "Chicken Feeding Standards", and includes corn, soybean meal, rock powder, calcium hydrogen phosphate, vitamins and mineral premixes.

3. A method for applying the laying hen feed additive according to claim 1 or 2, characterized in that: The feed additive is continuously fed to laying hens for at least 4 weeks, with a daily feeding amount of 100-120 g / hen, and the feeding stage is the laying period of laying hens at 20-60 weeks of age.

4. The application method of the laying hen feed additive according to claim 3, characterized in that: The preferred addition amount of the leafy grass powder is 10%, at which point the cholesterol content of eggs is reduced by 58%-60%, and the egg production rate is ≥90%.