A traditional Chinese medicine preparation for enhancing immunity and its preparation method

Abstract

This invention belongs to the field of traditional Chinese medicine, and particularly relates to a traditional Chinese medicine preparation for enhancing immunity and its preparation method. The preparation comprises the following raw materials in parts by weight: 10-18 parts red ginseng, 8-15 parts *Rhodiola rosea* root, 5-10 parts wolfberry, 1-5 parts *Hemiberlesia lingua*, 2-5 parts angelica dahurica, 2-5 parts *Polygonatum odoratum*, 3-10 parts rose, 1-5 parts red dates, and 1-3 parts brown sugar. This preparation enhances the immune organs, phagocytic coefficient, and ConA-induced splenic lymphocyte transformation capacity in normal mice.

Description

Technical Field

[0001] The present invention belongs to the field of traditional Chinese medicine, and particularly relates to a traditional Chinese medicine preparation for enhancing immunity and its preparation method. Background Art

[0002] Sub-health refers to a state of the human body between health and disease, characterized by a decrease in vitality, reduced function and adaptability within a certain period of time, but not yet reaching the diagnostic criteria of diseases. This state may involve multiple aspects such as physiology, psychology and social adaptation, and is specifically manifested as symptoms such as fatigue, insomnia, mood swings, memory decline, and low immunity. Low immunity means that the function of the human immune system is weakened, resulting in a decline in the body's defense ability against external pathogens (such as bacteria and viruses), as well as a weakened ability to monitor and eliminate internal abnormal cells (such as cancer cells).

[0003] Traditional Chinese medicine believes that low immunity is closely related to insufficient healthy qi. Healthy qi is the ability of the human body to resist external pathogens and maintain health, and its strength depends on the functions of the zang-fu organs (especially the spleen, lungs and kidneys) and the state of qi and blood. Traditional Chinese medicine emphasizes "strengthening healthy qi and consolidating the root", and enhances immunity by regulating the functions of the zang-fu organs and tonifying qi and blood. However, traditional Chinese medicine is difficult to extract, easily decomposed, and is prone to undergo the first-pass effect in the liver and decomposition in the gastrointestinal tract when taken orally, resulting in slow efficacy. There are many traditional Chinese medicines with immune-enhancing effects, such as ginseng, bird's nest, wolfberry, angelica, almond, pilose antler, curculigo rhizome, etc., but single herbs usually can only control or relieve certain symptoms, and have defects such as poor efficacy. Traditional Chinese medicine compound preparations, through the theory of compatibility of traditional Chinese medicines of monarch, minister, assistant and guide, enable synergistic effects among various herbs, and thus achieve effects such as improving efficacy and reducing toxic and side effects, which is the main development direction of future traditional Chinese medicine compound preparations.

[0004] Common prescriptions for strengthening healthy qi and consolidating the root include Sijunzi Decoction, Liuwei Dihuang Pills, etc. "Sijunzi Decoction" is a basic prescription for replenishing qi, which can improve the phagocytic ability of macrophages and is used to treat qi deficiency of the spleen and stomach and yin deficiency with internal heat, and is not suitable for those with abdominal distension; "Liuwei Dihuang Pills" is a prescription for nourishing yin, which can enhance the phagocytic ability of mononuclear macrophages and the proliferation of T and B lymphocytes, and is mainly used to treat kidney yin deficiency or liver-kidney yin deficiency. However, most of the herbs in the prescription are yin-nourishing herbs, and are not suitable for yang deficiency patients. Therefore, it is an urgent technical problem for those skilled in the art to develop a traditional Chinese medicine compound for enhancing the body's immunity with high efficiency, safety, wide applicable population, mild medicinal properties and suitable for long-term use. Summary of the Invention

[0005] In order to overcome the deficiencies of the prior art, one of the purposes of the present invention is to provide a traditional Chinese medicine preparation for enhancing immunity, which can enhance the immunity of normal mice.

[0006] One of the purposes of the present invention is achieved by adopting the following technical solutions:

[0007] A traditional Chinese medicine preparation for enhancing immunity comprises the following raw materials in parts by weight: 10-18 parts red ginseng, 8-15 parts Rhododendron molle root, 5-10 parts wolfberry, 1-5 parts red heart grass, 2-5 parts angelica dahurica, 2-5 parts Solomon's seal rhizome, 3-10 parts rose petals, 1-5 parts red dates, and 1-3 parts brown sugar.

[0008] Furthermore, the ingredients are composed of the following parts by weight: 12 parts red ginseng, 11 parts Rhododendron molle root, 7 parts wolfberry, 4 parts red heart grass, 3 parts angelica dahurica, 4 parts Solomon's seal rhizome, 9 parts rose petals, 4 parts red dates, and 2 parts brown sugar.

[0009] The second objective of this invention is to provide a method for preparing a traditional Chinese medicine preparation that enhances immunity, which is simple and easy to implement.

[0010] The second objective of this invention is achieved by the following technical solution:

[0011] The preparation method of the above-mentioned traditional Chinese medicine preparation for enhancing immunity includes the following steps:

[0012] (1) Weigh each ingredient according to the above weight ratio, add red ginseng, Rhodiola rosea root, wolfberry, red heart grass, angelica dahurica, Solomon's seal, rose, and red dates to water and decoct. After filtration, obtain the first filtrate and the first residue.

[0013] (2) Add water to the first filter residue from step (1) and boil it. After boiling, add brown sugar and mix evenly. Filter to obtain the second filtrate and the second filter residue. Combine the first filtrate and the second filtrate and concentrate them into an extract. Then add pharmaceutically acceptable excipients to make the corresponding dosage form.

[0014] Furthermore, the amount of water used in steps (1) and (2) is 5 to 10 times the total weight of the raw materials.

[0015] Furthermore, the decocting time in steps (1) and (2) is 1.5 to 2.5 hours.

[0016] Furthermore, the concentration temperature in step (2) is 55–65°C.

[0017] Furthermore, the relative density of the extract described in step (2) is 1.2 to 1.3.

[0018] Furthermore, the dosage form described in step (2) is an oral preparation.

[0019] Furthermore, the oral preparation is one of tablets, granules, or capsules.

[0020] The pharmacological and pharmacodynamic analyses of the active pharmaceutical ingredient used in this invention are as follows:

[0021] Red ginseng: [Properties and Channels Entered] Sweet and slightly bitter in taste, warm in nature. Enters the spleen, lung, and heart channels. [Functions and Indications] Replenishes vital energy, restores pulse and consolidates the body, benefits qi and controls blood loss; mainly used for weakness and impending collapse, cold limbs and weak pulse, qi failing to control blood loss, metrorrhagia, heart failure, and cardiogenic shock.

[0022] *Rhodiola rosea* root:

Properties and Channels Entered

Functions and Indications

[0023] Goji berries: [Properties and Channels Entered] Sweet and neutral in nature. Enters the Liver and Kidney channels. [Functions and Indications] Nourishes the Liver and Kidneys, benefits essence and improves eyesight; treats weakness and deficiency of essence, soreness and pain in the lower back and knees, dizziness and tinnitus, internal heat and thirst, blood deficiency and chlorosis, and blurred vision.

[0024] Red Heart Grass: Also known as Blue Cloth Grass, [Properties and Channels Entered] Pungent and slightly cold in nature; enters the Lung Channel. [Functions and Indications] Clears heat and detoxifies, eliminates carbuncles and drains pus, promotes urination and relieves strangury; mainly used to treat lung abscess with purulent sputum, cough with phlegm and heat, dysentery, strangury, carbuncles and boils.

[0025] Angelica dahurica: [Properties and Channels Entered] Warm in nature, pungent and slightly sweet in taste. Enters the Lung, Stomach, and Large Intestine channels. [Functions and Indications] Relieves exterior syndromes and dispels cold, dispels wind and relieves pain, clears nasal passages, dries dampness and stops leukorrhea, reduces swelling and drains pus, dispels wind and relieves itching. Mainly used to treat common cold due to wind-cold, headache, toothache, numbness and pain, sinusitis, leukorrhea, carbuncles and boils, and rheumatic itching of the skin.

[0026] Polygonatum odoratum: [Properties and Channels Entered] Neutral in nature, sweet in taste. Enters the Lung and Stomach channels. [Functions and Indications] Nourishes Yin and moistens dryness, generates fluids and quenches thirst. Primarily used to treat Lung and Stomach Yin deficiency, dry cough due to heat, dry throat and thirst, and internal heat with thirst.

[0027] Rose: [Properties and Channels Entered] Sweet and slightly bitter in taste, warm in nature. [Functions and Indications] Promotes qi circulation and relieves depression, harmonizes blood and dispels blood stasis; mainly used to treat liver and stomach qi pain, poor appetite and nausea, irregular menstruation, and injuries from falls and blows.

[0028] Red dates: [Properties and Channels Entered] Sweet in taste, warm in nature. Enters the spleen and stomach channels. [Functions and Indications] Tonifies the spleen and stomach, replenishes qi and generates fluids, regulates the body's defenses, and detoxifies; mainly used for stomach deficiency with poor appetite, spleen weakness with loose stools, insufficient qi, blood and body fluids, disharmony between the body's defenses, and palpitations.

[0029] Brown sugar: [Properties and Channels Entered] Sweet in taste, warm in nature, non-toxic. Enters the Liver and Spleen channels. [Functions and Indications] Nourishes the heart and lungs, harmonizes the middle jiao and assists the spleen, soothes liver qi, detoxifies alcohol, replenishes blood, and breaks up blood stasis; mainly treats abdominal distension due to heat, dry mouth with thirst, sore throat, cough due to lung heat, heat in the heart, lungs, large and small intestines, and alcohol poisoning.

[0030] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0031] The formula uses red ginseng and *Rhodiola rosea* root as the principal herbs. Red ginseng restores the pulse, consolidates the body, invigorates qi, and stops bleeding, while *Rhodiola rosea* root nourishes blood and benefits the kidneys. Goji berries and *Heliotropium indicum* are the assistant herbs. Goji berries nourish the liver and kidneys, while *Heliotropium indicum* invigorates qi, strengthens the spleen, nourishes blood, and nourishes yin. Angelica dahurica, Polygonatum odoratum, rose petals, and jujubes are the adjuvant herbs. Angelica dahurica nourishes yin and moistens dryness, Polygonatum odoratum dispels wind and dampness, rose petals regulate qi and relieve depression, and jujubes invigorate the middle jiao, replenish qi, nourish blood, and calm the mind. Brown sugar is the guiding herb, warming the middle jiao, assisting the spleen, and tonifying the middle jiao while soothing the liver. The combined effects of these herbs, by invigorating qi, strengthening the spleen, promoting body fluids, and activating blood circulation, strengthen the body and enhance immunity. Detailed Implementation

[0032] The present invention will be further described below with reference to specific embodiments. It should be noted that, without conflict, the various embodiments or technical features described below can be arbitrarily combined to form new embodiments. Specific conditions not specified in the embodiments shall be performed according to conventional conditions or conditions recommended by the manufacturer. Unless otherwise specified, all reagents or instruments used are conventional products obtained through commercial channels.

[0033] Example

[0034] Example 1

[0035] This embodiment provides a traditional Chinese medicine preparation for improving immunity, which comprises the following raw materials in parts by weight: 12 parts red ginseng, 11 parts Rhododendron molle root, 7 parts wolfberry, 4 parts red heart grass, 3 parts angelica dahurica, 4 parts Solomon's seal rhizome, 9 parts rose, 4 parts red dates, and 2 parts brown sugar.

[0036] This embodiment also provides a method for preparing the above-mentioned traditional Chinese medicine preparation, and the detailed preparation steps are as follows:

[0037] (1) Weigh each raw material according to the above weight ratio, and add red ginseng, Rhodiola rosea root, wolfberry, red heart grass, angelica dahurica, Solomon's seal, rose, and red dates to water and decoct for 2 hours according to the amount of water used being 8 times the total weight of the raw materials. After filtration, obtain the first filtrate and the first residue.

[0038] (2) Add water to the first filter residue from step (1) at a rate of 8 times the total weight of the raw materials and decoct for 2 hours. After decoction, add brown sugar and mix well. Filter to obtain the second filtrate and the second filter residue. Combine the first and second filtrates and concentrate to an extract with a relative density of 1.2. Add 2% starch by weight of the extract and prepare capsules according to conventional methods.

[0039] Example 2

[0040] This embodiment provides a traditional Chinese medicine preparation for improving immunity, which comprises the following raw materials in parts by weight: 18 parts red ginseng, 15 parts Rhododendron molle root, 10 parts wolfberry, 5 parts red heart grass, 5 parts angelica dahurica, 5 parts Solomon's seal rhizome, 10 parts rose, 5 parts red dates, and 3 parts brown sugar.

[0041] This embodiment also provides a method for preparing the above-mentioned traditional Chinese medicine preparation, and the detailed preparation steps are as follows:

[0042] (1) Weigh each raw material according to the above weight ratio, and add red ginseng, Rhodiola rosea root, wolfberry, red heart grass, angelica dahurica, Solomon's seal, rose, and red dates to water and decoct for 2.5 hours. After filtration, obtain the first filtrate and the first residue.

[0043] (2) Add water to the first filter residue from step (1) at a rate of 10 times the total weight of the raw materials and decoct for 2.5 hours. After decoction, add brown sugar and mix well. Filter to obtain the second filtrate and the second filter residue. Combine the first and second filtrates and concentrate to an extract with a relative density of 1.3. Add 2% starch by weight of the extract and prepare tablets according to conventional methods.

[0044] Example 3

[0045] This embodiment provides a traditional Chinese medicine preparation for improving immunity, which comprises the following raw materials in parts by weight: 10 parts red ginseng, 8 parts Rhododendron molle root, 5 parts wolfberry, 1 part red heart grass, 2 parts angelica dahurica, 2 parts Solomon's seal rhizome, 3 parts rose, 1 part red date, and 1 part brown sugar.

[0046] This embodiment also provides a method for preparing the above-mentioned traditional Chinese medicine preparation, and the detailed preparation steps are as follows:

[0047] (1) Weigh each raw material according to the above weight ratio, and add red ginseng, Rhodiola rosea root, wolfberry, red heart grass, angelica dahurica, Solomon's seal, rose, and red dates to water and decoct for 1.5 hours. After filtration, obtain the first filtrate and the first residue.

[0048] (2) Add water to the first filter residue from step (1) at a rate of 5 times the total weight of the raw materials and decoct for 1.5 hours. After decoction, add brown sugar and mix well. Filter to obtain the second filtrate and the second filter residue. Combine the first and second filtrates and concentrate to an extract with a relative density of 1.2. Add 1% dextrin and 1% sucrose by weight of the extract and prepare granules according to conventional methods.

[0049] Comparative Example

[0050] Comparative Example 1

[0051] Comparative Example 1 provides a traditional Chinese medicine preparation comprising the following raw materials in parts by weight: 12 parts red ginseng, 7 parts wolfberry, 4 parts red heart grass, 3 parts angelica dahurica, 4 parts Solomon's seal rhizome, 9 parts rose, 4 parts red dates, and 2 parts brown sugar.

[0052] Comparative Example 1 also provides a method for preparing the above-mentioned traditional Chinese medicine preparation, with detailed preparation steps and conditions consistent with those in Example 1.

[0053] Comparative Example 2

[0054] Comparative Example 2 provides a traditional Chinese medicine preparation comprising the following raw materials in parts by weight: 23 parts red ginseng, 7 parts wolfberry, 4 parts red heart grass, 3 parts angelica dahurica, 4 parts Solomon's seal rhizome, 9 parts rose, 4 parts red dates, and 2 parts brown sugar.

[0055] Comparative Example 2 also provides a method for preparing the above-mentioned traditional Chinese medicine preparation, with detailed preparation steps and conditions consistent with those in Example 1.

[0056] Comparative Example 3

[0057] Comparative Example 3 provides a traditional Chinese medicine preparation comprising the following raw materials in parts by weight: 12 parts red ginseng, 11 parts Rhododendron molle root, 7 parts wolfberry, 3 parts Angelica dahurica, 4 parts Polygonatum odoratum, 9 parts rose, 4 parts red dates, and 2 parts brown sugar.

[0058] Comparative Example 3 also provides a method for preparing the above-mentioned traditional Chinese medicine preparation, with detailed preparation steps and conditions consistent with those in Example 1.

[0059] Comparative Example 4

[0060] Comparative Example 4 provides a traditional Chinese medicine preparation comprising the following raw materials in parts by weight: 12 parts red ginseng, 11 parts Rhododendron molle root, 11 parts wolfberry, 3 parts Angelica dahurica, 4 parts Polygonatum odoratum, 9 parts rose, 4 parts red dates, and 2 parts brown sugar.

[0061] Comparative Example 4 also provides a method for preparing the above-mentioned traditional Chinese medicine preparation, with detailed preparation steps and conditions consistent with those in Example 1.

[0062] The efficacy of the traditional Chinese medicine preparations obtained in Examples 1-3 and Comparative Examples 1-4 is described below, with detailed procedures as follows:

[0063] (1) Laboratory animals

[0064] A total of 80 SPF-grade Balb / c nude mice were used, with a male-to-female ratio of 1:1 and a weight of 18-22g.

[0065] (2) Experimental Grouping

[0066] All experimental animals were randomly divided into the low, medium and high dose group of Example 1, the high dose group of Comparative Example 1, the high dose group of Comparative Example 2, the high dose group of Comparative Example 3, the high dose group of Comparative Example 4, and the normal control group, with 10 animals in each group.

[0067] (3) Administration method

[0068] Example 1 Low-dose group: The traditional Chinese medicine preparation of Example 1 was administered by gavage at a dose of 5 g / kg (crude drug) once a day for 30 consecutive days;

[0069] In Example 1, the dosage group was administered the traditional Chinese medicine preparation of Example 1 by gavage at a dose of 10 g / kg (crude drug) once a day for 30 consecutive days.

[0070] Example 1 High-dose group: The traditional Chinese medicine preparation of Example 1 was administered by gavage at a dose of 20g / kg (crude drug) once a day for 30 consecutive days;

[0071] High-dose group of Comparative Example 1: The traditional Chinese medicine preparation of Comparative Example 1 was administered by gavage at a dose of 20 g / kg (crude drug) once a day for 30 consecutive days.

[0072] High-dose group of Comparative Example 2: The traditional Chinese medicine preparation of Comparative Example 2 was administered by gavage at a dose of 20 g / kg (crude drug) once a day for 30 consecutive days.

[0073] High-dose group of Comparative Example 3: The traditional Chinese medicine preparation of Comparative Example 3 was administered by gavage at a dose of 20 g / kg (crude drug) once a day for 30 consecutive days;

[0074] High-dose group of Comparative Example 4: The traditional Chinese medicine preparation of Comparative Example 4 was administered by gavage at a dose of 20 g / kg (crude drug) once a day for 30 consecutive days.

[0075] Normal control group: The same volume of normal saline was administered by gavage once a day for 30 consecutive days.

[0076] (4) Detection indicators

[0077] a. Organ Index

[0078] The weight of mice in each group was measured and recorded daily. The spleen and thymus of the euthanized mice were dissected, rinsed with physiological saline, blotted dry with filter paper, weighed, and the organ index was calculated using the following formula:

[0079] Organ index = organ mass (mg) / body weight (g), the results are shown in Table 1.

[0080] b. Phagocytic function of phagocytes

[0081] The phagocytic function of phagocytes was assessed using the carbon particle clearance method. The specific detection method is as follows:

[0082] Thirty minutes after the last administration, 0.05 mL / 10 g of 3-fold diluted Indian ink was injected into the tail vein of mice in each group. At 30 s (t1) and 360 s (t2), 20 μL of blood was collected from behind the eye socket and added to 2 mL of 0.1% Na2CO3 solution. The optical density was measured at 680 nm. OD1 represents the optical density at 30 s, and OD2 represents the optical density at 360 s. The clearance index K was calculated using the formula. After converting the K value to body weight and liver / spleen weight, the phagocytic coefficient was obtained. The results are shown in Table 2.

[0083] Clarification index K = (lgOD1 - lgOD2) / (t2 - t1)

[0084] c. Lymphocyte transformation capacity

[0085] The detection was performed using the ConA-induced mouse spleen lymphocyte transformation assay (MTT method), and the specific detection method is as follows:

[0086] The spleens of mice in each group were ground up and made into cell suspensions, and the cell concentration was adjusted to 3×10⁻⁶. 5 Cells / mL. Each group of cell suspensions was added to two wells of a 24-well plate, 1 mL per well. One well contained 75 μL of ConA solution (well A), and the other well contained an equal volume of FBS-free RPMI 1640 medium (well B). Three replicates were set up for each group. The 24-well plates were incubated at 37°C with 5% CO2 for 72 h. Four h before the end of the incubation period, 0.7 mL of supernatant was gently aspirated from each well, and 0.7 mL of FBS-free RPMI 1640 medium was added. Simultaneously, 50 μL of MTT (5 mg / mL) was added to each well, and the plates were incubated for another 4 h. After incubation, 1 mL of acidic isopropanol was added to each well, and the mixture was pipetted to dissolve the purple crystals. The optical density of each well was measured at 570 nm. Control wells containing only the cell suspensions and MTT solution were also included. The formula for calculating the lymphocyte transformation capacity is as follows:

[0087] Lymphocyte transformation capacity (%) = (A well) OD - Reference hole OD ) / (B hole OD - Reference hole OD The results are shown in Table 2.

[0088] Table 1

[0089]

[0090]

[0091] Table 2

[0092] Grouping dose Devouring coefficient Lymphocyte transformation capacity (%) normal control group / 5.38 18.22 Example 1 Low-dose group 5g / kg 5.74 24.01 In Example 1, the dosage group 10g / kg 6.19 26.30 Example 1 High-dose group 20g / kg 6.43 28.02 Comparative Example 1 High-dose group 20g / kg 5.45 19.43 Comparative Example 2 High-Dose Group 20g / kg 5.57 20.65 Comparative Example 3 High-Dose Group 20g / kg 5.60 22.38 Comparative Example 4 High-Dose Group 20g / kg 5.63 23.54

[0093] As shown in Table 1, the traditional Chinese medicine preparation obtained in this invention can significantly increase the organ coefficient of the immune organs of normal mice, that is, the traditional Chinese medicine preparation obtained in this invention has an enhancing effect on the immune organs of normal mice.

[0094] As shown in Table 2, the traditional Chinese medicine preparation obtained in this invention can significantly enhance the transformation ability of ConA-induced splenic lymphocytes in mice and the phagocytic coefficient in normal mice. This indicates that the traditional Chinese medicine preparation obtained in this invention enhances the lymphocyte transformation ability and macrophage phagocytic function of normal mice. In summary, the traditional Chinese medicine preparation obtained in this invention can improve the immunity of normal mice.

[0095] Compared to Example 1, Comparative Example 1 omitted the root of *Rhodiola rosea*, Comparative Example 2 omitted the root of *Rhodiola rosea* and increased the dosage of red ginseng, Comparative Example 3 omitted *Smilax glabra*, and Comparative Example 4 omitted *Smilax glabra* and increased the dosage of wolfberry. The resulting herbal preparations showed a reduced effect on enhancing the immunity of normal mice. These results indicate that the combination of *Rhodiola rosea* root and red ginseng acts as the principal ingredient, while wolfberry and *Smilax glabra* act as the assistant ingredients, effectively enhancing the immunity of normal mice.

[0096] The above embodiments are merely preferred embodiments of the present invention and should not be construed as limiting the scope of protection of the present invention. Any non-substantial changes and substitutions made by those skilled in the art based on the present invention shall fall within the scope of protection claimed by the present invention.

Claims

1. A traditional Chinese medicine preparation for enhancing immunity, characterized in that, It is made from the following ingredients in parts by weight: 10-18 parts red ginseng, 8-15 parts Rhododendron molle root, 5-10 parts wolfberry, 1-5 parts red heart grass, 2-5 parts angelica dahurica, 2-5 parts Solomon's seal rhizome, 3-10 parts rose petals, 1-5 parts red dates, and 1-3 parts brown sugar.

2. The traditional Chinese medicine preparation for enhancing immunity according to claim 1, characterized in that, It is made from the following ingredients by weight: 12 parts red ginseng, 11 parts Rhododendron molle root, 7 parts wolfberry, 4 parts red heart grass, 3 parts angelica dahurica, 4 parts Solomon's seal rhizome, 9 parts rose petals, 4 parts red dates, and 2 parts brown sugar.

3. The method for preparing the traditional Chinese medicine preparation for enhancing immunity according to claim 1 or 2, characterized in that, Includes the following steps: (1) Weigh each ingredient according to the above weight ratio, add red ginseng, Rhodiola rosea root, wolfberry, red heart grass, angelica dahurica, Solomon's seal, rose, and red dates to water and decoct. After filtration, obtain the first filtrate and the first residue. (2) Add water to the first filter residue in step (1) and boil it. After boiling, add brown sugar and mix evenly. After filtering, obtain the second filtrate and the second filter residue. Combine the first filtrate and the second filtrate and concentrate them into an extract. Then add pharmaceutically acceptable excipients to make the corresponding dosage form.

4. The method for preparing the traditional Chinese medicine preparation for enhancing immunity according to claim 3, characterized in that, The amount of water used in steps (1) and (2) is 5 to 10 times the total weight of the raw materials.

5. The method for preparing the traditional Chinese medicine preparation for enhancing immunity according to claim 3, characterized in that, The simmering time in steps (1) and (2) is 1.5 to 2.5 hours.

6. The method for preparing the traditional Chinese medicine preparation for enhancing immunity according to claim 3, characterized in that, The concentration temperature in step (2) is 55~65℃.

7. The method for preparing the traditional Chinese medicine preparation for enhancing immunity according to claim 3, characterized in that, The relative density of the extract in step (2) is 1.2 to 1.

3.

8. The method for preparing the traditional Chinese medicine preparation for enhancing immunity according to claim 3, characterized in that, The dosage form mentioned in step (2) is an oral preparation.

9. The method for preparing the traditional Chinese medicine preparation for enhancing immunity according to claim 8, characterized in that, The oral preparation is one of tablets, granules, or capsules.

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