Traditional Chinese medicine composition for treating sheep cold-dampness diarrhea as well as preparation method and application of traditional Chinese medicine composition

Abstract

The invention discloses a traditional Chinese medicine composition for treating sheep cold-dampness diarrhea and a preparation method and application thereof. Belongs to the field of veterinary medicine preparation. The traditional Chinese medicine composition is prepared from rhizoma zingiberis, limonite, radix aucklandiae, flos lonicerae, pericarpium citri reticulatae, rhizoma atractylodis macrocephalae, betel nuts, radix astragali and radix glycyrrhizae preparata. The preparation method comprises the following steps: soaking the raw materials in distilled water with the volume 3 times that of the raw materials; after boiling, decocting with slow fire, and filtering liquid medicine; adding distilled water of which the volume is 3 times that of the filtered medicine residues into the filtered medicine residues, boiling, continuing to decoct with slow fire, filtering medicine liquid with a 80-mesh filter sieve, combining two decoctions, concentrating with slow fire, and filtering to finally prepare the water decoction. The traditional Chinese medicine composition has the effects of warming yang, dispelling cold, eliminating dampness, eliminating stagnation, invigorating spleen, replenishing qi and astringing to arrest diarrhea, has a good treatment effect on sheep cold-dampness diarrhea, and is free of toxic and side effects.

Description

Technical Field

[0001] The present invention belongs to the technical field of veterinary medicine preparation. Specifically, it relates to a traditional Chinese medicine composition for treating cold-damp diarrhea in sheep, its preparation method and application. Background Art

[0002] Diarrhea is one of the common diseases in sheep breeding. Its causes are complex and diverse, mainly including infectious factors such as bacterial, viral or parasitic infections; non-infectious factors such as acute gastroenteritis, ingestion of excessive feed that is prone to produce gas and acid, or gastrointestinal dysfunction caused by various reasons; drug stimulation factors and food poisoning factors, etc. While pursuing the maximization of interests, the highly intensive breeding mode inevitably sacrifices some welfare needs of animals, such as small activity space, poor environmental conditions, lack of individual guardianship, etc., resulting in a decrease in the body's immunity of animals. The incidence of stress (non-infectious) diseases in livestock and poultry breeding is increasing continuously. Among them, gastrointestinal diseases are highly prevalent, and the most common symptom is diarrhea.

[0003] Cold-damp diarrhea is a common type of diarrhea, which is a disease syndrome mainly characterized by increased frequency of defecation, loose stools, and even diarrhea like water caused by excessive internal cold-dampness. Cold-damp diarrhea is generally caused by cold and humid environment, sudden drop in temperature, overeating frozen feed or excessive drinking of cold underground water in hot weather. Traditional medicine believes that the spleen and stomach are the foundation of acquired constitution. Weakness of the spleen and stomach will lead to a decline in the transportation and transformation function, indigestion of food and water, endogenous dampness, and trigger diarrhea. Cold-damp diarrhea mostly occurs in lambs. When it occurs, it is often accompanied by loss of appetite, emaciation, disordered hair coat, pale oral mucosa, etc. In severe cases, mental fatigue, arched back and fear of cold, cold limbs, unsteady gait, pale mucosa, etc. can be seen, and even death may occur. Cold-damp diarrhea often leads to a decrease in the resistance of affected sheep and causes secondary infections, especially the conditional pathogenic bacteria resident in the breeding farm, thus increasing the treatment difficulty and bringing huge economic losses, seriously restricting the development of the sheep breeding industry.

[0004] At present, the veterinary drug products for treating sheep diarrhea in veterinary clinics are mainly anti-infective drugs, such as antibiotics, antiviral drugs, anti-parasitic drugs, antidiarrheal drugs, anti-inflammatory drugs, etc. Most of them are chemically synthesized drugs and are targeted at pathogenic microorganisms. Although these drugs can effectively prevent and control secondary infections, their therapeutic effect on cold-damp diarrhea is limited because they cannot effectively improve the spleen and stomach function of affected sheep and the internal environment of the body. Especially for lambs with weak constitutions, frequent diarrhea will lead to a large amount of body fluid loss, resulting in dehydration, electrolyte imbalance and digestive system dysfunction, and is prone to cause death, resulting in huge economic losses. In addition, long-term application of antibiotics will further induce the imbalance of the intestinal flora of affected sheep and even lead to the generation of bacterial drug resistance, increasing the difficulty of preventing and controlling related diseases. Therefore, during the livestock and poultry breeding process, the demand for safe, effective and green new drugs is very urgent. Summary of the Invention

[0005] Objective of the Invention: The objective of the present invention is to provide a traditional Chinese medicine composition for treating cold-damp diarrhea in sheep, its preparation method and application; aiming to provide a new option for the treatment of sheep diarrhea, especially to reduce or replace the use of antibiotics in livestock and poultry farming, and avoid the long-term application or abuse of antibiotics; to alleviate problems such as the drug resistance of pathogenic microorganisms and the food safety and ecological risks brought by drug residues.

[0006] Technical Solution of the Invention: The traditional Chinese medicine composition for treating cold-damp diarrhea in sheep described in the present invention has the following mass components: 13 - 16 parts of dried ginger, 5 - 8 parts of limonitum, 4 - 8 parts of costus root, 8 - 12 parts of honeysuckle flower, 5 - 7 parts of dried tangerine peel, 4 - 8 parts of atractylodes rhizome, 5 - 7 parts of areca nut, 9 - 11 parts of astragalus membranaceus and 5 - 7 parts of roasted licorice root.

[0007] Further, the mass components of the traditional Chinese medicine composition are as follows: 15 parts of dried ginger, 6 parts of limonitum, 6 parts of costus root, 10 parts of honeysuckle flower, 6 parts of dried tangerine peel, 6 parts of atractylodes rhizome, 6 parts of areca nut, 10 parts of astragalus membranaceus and 6 parts of roasted licorice root.

[0008] The present invention also provides a preparation method of the traditional Chinese medicine composition for treating cold-damp diarrhea in sheep, and its preparation steps are as follows:

[0009] Step (1): Add the raw materials to be decocted into 3 times the volume of distilled water and soak for 1 h to make them fully absorb water.

[0010] Step (2): After soaking, use high heat to boil the raw materials, and immediately change to low heat and continue to decoct for 2 h after boiling. After decoction, filter the medicinal residues with an 80-mesh filter screen and collect the medicinal liquid. The filtered medicinal residues are for re-decoction.

[0011] Step (3): Add 3 times the volume of distilled water to the medicinal residues, use high heat to boil the medicinal residues, and immediately change to low heat and continue to decoct for 2 h after boiling. After decoction, filter the medicinal residues with an 80-mesh filter screen, discard the residues and collect the medicinal liquid. Combine the two decocted liquids and use low heat to concentrate the decocted liquid to the same volume as the raw materials to make the final water decoction.

[0012] The present invention also provides the application of the above-mentioned traditional Chinese medicine composition for treating cold-damp diarrhea in sheep in the preparation of drugs for improving the symptoms of perianal fecal contamination in diarrheal sheep.

[0013] The present invention also provides the application of the above-mentioned traditional Chinese medicine composition for treating cold-damp diarrhea in sheep in the preparation of drugs for reducing the diarrhea rate in sheep.

[0014] The present invention also provides the application of the above-mentioned traditional Chinese medicine composition for treating cold-damp diarrhea in sheep in the preparation of drugs for reducing the diarrhea index in sheep;

[0015] Among them, the diarrhea index refers to: observing the feces of the experimental lambs daily and scoring them. If the feces are in the shape of strips or grains, it is recorded as 1 point; if the feces are soft and can form, it is recorded as 2 points; if the feces are thick and unformed, and the feces and water are not separated, it is recorded as 3 points; if the feces are liquid and unformed, and the feces and water are separated, it is recorded as 4 points. When the feces score > 2 points, it is judged as diarrhea, and when ≤ 2 points, it is judged as cured.

[0016] The present invention also provides the application of the above-mentioned traditional Chinese medicine composition for treating cold-damp diarrhea in sheep in the preparation of a drug for improving the abnormal blood routine of diarrhea sheep; the blood routine includes white blood cells, platelet count and hemoglobin.

[0017] The present invention also provides the application of the above-mentioned traditional Chinese medicine composition for treating cold-damp diarrhea in sheep in the preparation of a drug for improving the abnormal blood biochemistry of diarrhea sheep; the blood biochemistry includes alkaline phosphatase, globulin, urea, total protein, and albumin / globulin ratio.

[0018] The traditional Chinese medicine composition for treating cold-damp diarrhea in sheep is composed of dried ginger, limonitum, costus root, honeysuckle flower, dried tangerine peel, atractylodes rhizome, areca nut, astragalus membranaceus and roasted licorice root;

[0019] Among them, dried ginger has the effects of warming yang and dispelling cold, and restoring yang and rescuing from collapse, and can warm the spleen and stomach, dispel cold and remove dampness;

[0020] Limonitum has the effects of astringing the intestine to stop diarrhea and arresting bleeding by astringency;

[0021] Costus root and areca nut have the effects of awakening the spleen and promoting appetite, promoting qi movement and relieving stagnation, and drying dampness and resolving accumulation;

[0022] Honeysuckle flower has the effects of clearing heat and detoxifying, cooling blood and detumescence, and helps to remove dampness and relieve stagnation, and improve diarrhea symptoms;

[0023] Dried tangerine peel has the effects of promoting qi movement and resolving accumulation, drying dampness and reducing phlegm, and can help regulate qi movement and relieve stagnation, and eliminate dampness;

[0024] Atractylodes rhizome has the effects of strengthening the spleen and replenishing qi, and promoting diuresis and excreting dampness, and can effectively promote water and damp metabolism;

[0025] Astragalus membranaceus has the effects of benefiting qi and nourishing blood, promoting diuresis and reducing swelling, and promoting qi movement and dredging collaterals;

[0026] Roasted licorice root has the functions of invigorating the spleen and replenishing qi, and harmonizing various medicinal herbs.

[0027] Beneficial effects: 1. The traditional Chinese medicine composition of the present invention can effectively treat cold-damp diarrhea in sheep; the traditional Chinese medicine composition can play multiple roles such as warming yang and dispelling cold, drying dampness and resolving stagnation, strengthening the spleen and replenishing qi, and astringing diarrhea, helping the diseased sheep to remove the cold-damp stagnation in the body and restoring the spleen and stomach functions of the diseased sheep, thereby promoting appetite and eliminating the diarrhea symptoms of the diseased sheep; its treatment effect is better than that of antibiotics (commonly used clinically); 2. The traditional Chinese medicine composition of the present invention provides a new choice for preventing and treating diarrhea in the livestock and poultry breeding industry, is expected to reduce or replace the addition of anti-diarrhea drugs in livestock and poultry breeding, alleviate the generation of antibiotic resistance in livestock and poultry, and reduce food safety problems such as drug residues. Description of the Drawings

[0028] Figure 1 It is a comparison diagram of the perianal fecal contamination of sheep in each test group before and after treatment in the embodiment of the present invention; Figure A is a comparison diagram of the perianal fecal contamination of sheep in the traditional Chinese medicine treatment group before and after treatment; Figure B is a comparison diagram of the perianal fecal contamination of sheep in the antibiotic treatment group before and after treatment;

[0029] Figure 2 It is a comparison diagram of the diarrhea rates of sheep in each test group in the embodiment of the present invention;

[0030] Figure 3 It is a comparison diagram of the diarrhea indices of sheep in each test group in the embodiment of the present invention; (Note: Comparison among groups, *P < 0.05, **P < 0.01, ***P < 0.001; )

[0031] Figure 4 It is a comparison diagram of the average medication days of sheep in each test group in the embodiment of the present invention; (Note: Comparison among groups, *P < 0.05). Detailed Embodiments

[0032] The following further illustrates the present invention in conjunction with the drawings and specific embodiments.

[0033] A traditional Chinese medicine composition for treating cold-damp diarrhea in sheep in large-scale breeding according to the present invention, grab the traditional Chinese medicine composition in proportion: 15 g of dried ginger, 6 g of limonitum, 6 g of costus root, 10 g of honeysuckle, 6 g of dried tangerine peel, 6 g of atractylodes macrocephala, 6 g of areca nut, 10 g of astragalus membranaceus and 6 g of roasted licorice root as one dose, add 3 times the volume of distilled water and soak for 1.5 h; after boiling, decoct with slow fire for 2 h, and filter the medicinal liquid with an 80-mesh filter sieve; add 3 times the volume of distilled water to the medicinal residues, continue to decoct with slow fire for 2 h, and filter the medicinal liquid with an 80-mesh filter sieve. After combining the two decocted liquids, concentrate with slow fire, filter and make into a water decoction of 1 g / mL. Each adult diseased livestock takes one dose per day, and the dosage is adjusted according to the body weight as appropriate, and it is taken using a gastric gavage tool.

[0034] The traditional Chinese medicine preparation of the present invention has the effects of warming yang and dispelling cold, drying dampness and resolving stagnation, strengthening the spleen and replenishing qi, and astringing diarrhea. It has a good therapeutic effect on cold-damp diarrhea in sheep, does not affect the slaughter quality, and has small toxic and side effects.

[0035] Example 1

[0036] Therapeutic effects of different traditional Chinese medicine compositions on cold-damp diarrhea in sheep

[0037] 1. Method

[0038] 1.1 Experimental animals

[0039] A large-scale sheep farm in Taizhou City, Jiangsu Province, with an annual output of 7,000 sheep. In December 2022, during the feeding process, a large number of sheep showed diarrhea that persisted for a long time. After on-site investigation, it was found that the diseased livestock showed: lowering the head and drooping the ears, obvious coolness at the extremities of the four limbs, ears and nose, pale tongue, thin white and moist slippery tongue coating; presenting varying degrees of watery feces, or the feces were discharged in a jet-like manner.

[0040] Randomly select 10 healthy sheep from the same pen; randomly select 30 severely diseased sheep with diarrhea from the diarrhea flock in the same pen.

[0041] The feed formula was prepared according to the sheep nutrition standard in Table 1.

[0042] Table 1 Composition and nutritional level of the basal diet (air-dried basis)

[0043]

[0044] Note: Each 1 kg of premix can provide: VA 1.25×105 IU, VD 3 5×104 IU, VE 625 IU, VK 3 50 mg, VB 1 50 mg, VB 2 100 mg, VB 6 75 mg, VB 12 0.25 mg, nicotinamide 750 mg, pantothenic acid 250 mg, folic acid 15 mg, biotin 3.75 mg, choline 11 g, copper 0.03 g, iron 0.87 g, manganese 1 g, zinc 1 g, iodine 5 mg, selenium 5 mg, methionine 5%, calcium 15%, total phosphorus 5%, sodium chloride 8%, moisture ≤10%;

[0045] 1.2 Composition of traditional Chinese medicine composition and preparation of decoction

[0046] The traditional Chinese medicine composition consists of 15 g of dried ginger, 6 g of limonitum, 6 g of costus root, 10 g of honeysuckle flower, 6 g of tangerine peel, 6 g of atractylodes rhizome, 6 g of areca nut, 10 g of astragalus root and 6 g of roasted licorice root, and its efficacy is to warm yang and dispel cold, dry dampness and resolve stagnation, strengthen the spleen and replenish qi, and astringe diarrhea.

[0047] Weigh a total of 1000 g of the traditional Chinese medicine composition grabbed in proportion, add 3 times the volume of distilled water, soak for 1.5 h, boil, and then decoct with a gentle fire for 2 h. Filter the medicinal liquid with an 80-mesh sieve; add 3 times the volume of distilled water to the medicinal residues, continue to decoct with a gentle fire for 2 h, filter with an 80-mesh sieve, combine the two decocted liquids, and then concentrate to 1000 mL with a gentle fire. After filtration, prepare a water decoction of 1 g / mL.

[0048] 1.3. Experimental grouping and treatment methods

[0049] Randomly select 10 healthy sheep as the healthy control group; randomly select 30 severely diarrheal sheep from the diarrheal population in the same pen and randomly divide them into 3 groups: blank control group, traditional Chinese medicine treatment group, and antibiotic treatment group, with 10 sheep in each group, for a total of 30 sheep; the experimental sheep flocks are fed and managed in the same way every day. Among them, the sheep in the healthy control group are raised normally without intervention; the sheep in the blank control group are given 2 mL / kg of normal saline by gavage once a day for 7 consecutive days; the sheep in the traditional Chinese medicine treatment group are given 2 mL / kg of the water decoction of the traditional Chinese medicine by gavage once a day for 7 consecutive days; the sheep in the antibiotic treatment group are intramuscularly injected with amoxicillin (10 mg / kg) and enrofloxacin (2.5 mg / kg) once a day for 7 consecutive days.

[0050] When the feces of the sheep in each treatment group are formed (diarrhea index ≤ 2), the medication is stopped; during the experiment, take pictures of the perianal area of the sheep in each group every day, record the diarrhea index, and count the diarrhea rate; collect blood aseptically on the 0th and 7th days of the experiment for routine blood and blood biochemical index detection.

[0051] 1.4. Evaluation of the efficacy of the traditional Chinese medicine composition in improving perianal fecal contamination in diarrheal sheep

[0052] Take pictures of the perianal area of the diarrheal sheep in each experimental group every day and compare the changes after treatment with the traditional Chinese medicine composition and antibiotics.

[0053] 1.5. The traditional Chinese medicine composition reduces the diarrhea rate and diarrhea index of diarrheal sheep

[0054] Observe and record the feces of the experimental sheep every day and score them. Record feces in granular form as 1 point; record feces that are soft and can form as 2 points; record feces that are thick and unformed with no separation of feces and water as 3 points; record feces that are liquid, unformed, and with separation of feces and water as 4 points. Judge diarrhea when the feces score < 2 points and cure when ≤ 2 points.

[0055] 1.6. The traditional Chinese medicine composition reduces the average number of days of medication for diarrheal sheep

[0056] Diarrheal sheep are treated with the water decoction of the traditional Chinese medicine composition by gavage or antibiotic injection every day. When the feces are formed, they enter the cured state. Record the medication time of each sheep in each treatment group and calculate the average number of days of medication for each group.

[0057] 1.7 Improvement of abnormal blood routine indexes in diarrhea sheep by traditional Chinese medicine composition

[0058] Use EDTA-K 2 anticoagulant tubes, aseptically collect the blood of sheep in each group on the 0th and 7th days of the experiment and store it in an ice box. After collection, perform blood routine determination at the testing center of the Animal Hospital of Yangzhou University;

[0059] 1.8 Improvement of abnormal blood biochemical indexes in diarrhea sheep by traditional Chinese medicine composition

[0060] Use non-anticoagulant blood collection tubes, aseptically collect the blood of sheep in each group on the 0th and 7th days of the experiment and store it in an ice box. After centrifugation, collect the serum and perform blood biochemical index determination at the testing center of the Animal Hospital of a certain university;

[0061] 1.9 Data analysis

[0062] Use IBM SPSS Statistics 17.0 software for statistical analysis, and use Graphpad Prism 9.5.1 to make charts for experimental data. The data are all expressed as mean ± standard error (Mean ± SEM), and one-way ANOVA is used for comparison between groups. The standard for significant difference is P < 0.05;

[0063] 2. Experimental results

[0064] 2.1 Perianal fecal contamination of sheep in each experimental group

[0065] As Figure 1 shown, there was obvious loose stool around the anus in each experimental group on the 1st day; on the 7th day, the phenomenon of loose stool around the anus in each experimental group was significantly improved. Among them, the traditional Chinese medicine composition had the best treatment effect;

[0066] 2.2 Diarrhea rate of sheep in each experimental group

[0067] As Figure 2 shown, the diarrhea rate of sheep in the blank control group did not change significantly and remained at 100% all the time; the onset time of the traditional Chinese medicine treatment group was faster, and the diarrhea rate dropped to 50% on the 3rd day and to 0% on the 6th day; while after 7 days of antibiotic treatment, the diarrhea rate was still 20%;

[0068] 2.3 Diarrhea index of sheep in each experimental group

[0069] As Figure 3As shown, during the experiment, the diarrhea index of the sheep in the blank control group did not show obvious improvement, and the average diarrhea index was still 3.4 (not cured) at the end of the experiment; the diarrhea index of the sheep in the antibiotic treatment group decreased slowly, and the average diarrhea index was still higher than 2 (not cured) on the 7th day; the diarrhea index of the sheep in the traditional Chinese medicine treatment group decreased rapidly, and the average diarrhea index was 1.9 (cured) on the 5th day;

[0070] 2.4 Average number of days of medication for each experimental group of sheep

[0071] As Figure 4 shown, the average number of days of medication for the traditional Chinese medicine treatment group was 3.5 days, which was significantly lower than 5.3 days of the antibiotic treatment group (P<0.05);

[0072] 2.5 Results of blood routine indexes of each experimental group of sheep; see Table 2 for details;

[0073]

[0074] As shown in Table 2 (above table), compared with the healthy control group, the white blood cell and platelet counts of the sheep in the blank control group were significantly increased, while the red blood cell, hemoglobin, hematocrit, and mean corpuscular hemoglobin volume were significantly decreased; after treatment, the white blood cell and platelet counts of the traditional Chinese medicine treatment group and the antibiotic treatment group were significantly decreased, and the hemoglobin was significantly increased;

[0075] 2.6 Results of blood biochemical indexes of each experimental group of sheep; see Table 3 for details;

[0076]

[0077] As shown in Table 3 (see above table), compared with the healthy control group, the alkaline phosphatase, globulin, urea, and triglyceride of the sheep in the blank control group were significantly increased, while the total protein and albumin / globulin ratio were significantly decreased; after treatment, the alkaline phosphatase, globulin, and urea of the traditional Chinese medicine treatment group and the antibiotic treatment group of sheep were significantly decreased, and the total protein and albumin / globulin ratio were significantly increased;

[0078] 3. Summary

[0079] The traditional Chinese medicine composition showed high therapeutic effects in the treatment of diarrhea, significantly reducing the average medication time. The traditional Chinese medicine combination group had a positive effect on the inflammation and blood recovery of diarrhea sheep. The traditional Chinese medicine composition could promote the healing process of sheep diarrhea by reducing the white blood cell and platelet counts and increasing hemoglobin, and could improve the liver and kidney functions of diarrhea sheep, regulate blood sugar and lipid metabolism. Compared with antibiotic treatment, the traditional Chinese medicine composition had better effects, indicating that the traditional Chinese medicine composition might be a more effective diarrhea treatment method.

[0080] Example 2

[0081] Clinical application of traditional Chinese medicine composition in treating cold-damp diarrhea of sheep

[0082] This example is a clinical application test carried out by the Jiangsu Mutton Sheep Industry Demonstration Base in large-scale mutton sheep farms under the guidance of the Disease Prevention and Control Innovation Team of the Jiangsu Mutton Sheep Industry Technology System.

[0083] 1. Application results in Guannan Base

[0084] The traditional Chinese medicine composition was clinically applied to treat diarrhea in mutton sheep in a total of 13 sheep farms for data statistics (Tables 4 - 6). There were 39 diarrhea sheep in total, 35 were cured, 4 died, and the clinical recovery rate was 89.7%; the average number of medication times was 5.43 times, and the average recovery time was 2.37 days. The clinical treatment effect was significant, and the weight was increased to a certain extent (the difference was not significant); the dead sheep were cases with a long onset time or severe dehydration.

[0085] Table 4 Relevant information of the sheep farms in Guannan Base

[0086]

[0087] Table 5 Application of the traditional Chinese medicine composition in treating cold-damp diarrhea in sheep in Guannan Base

[0088]

[0089]

[0090] 2. Application results in Qidong Base

[0091] During the period from March to April 2023, the traditional Chinese medicine composition was clinically applied to treat diarrhea in mutton sheep in a total of 12 sheep farms. There were 65 diarrhea lambs in total, 63 were clinically recovered (the clinical recovery rate > 96.92%), and 2 showed no clinical effect; the average number of medication times was 4.48 times, and the average recovery time was 2.14 days. The clinical application effect was excellent; after application in a certain livestock farm in this area, partial data statistics were carried out. The results showed that the traditional Chinese medicine composition could treat diarrhea in mutton sheep (Hu sheep). After 3 days, the clinical recovery rate of the demonstration group was 100%, and the weight was increased to a certain extent (the difference was not significant).

[0092] Table 6 Application of the traditional Chinese medicine composition in treating cold-damp diarrhea in sheep in a certain livestock farm in this area

[0093]

[0094] 3. Application results in Haimen Base

[0095] During the period from March to May 2023, the traditional Chinese medicine composition was clinically applied to treat lamb diarrhea in 3 sheep farms (Table 7). A total of 39 diarrheal lambs were treated, and 37 lambs recovered clinically (clinical recovery rate > 94.87%), while 2 lambs showed no clinical effect; the average number of medication times was 4.86 times, and the average recovery time was 2.33 days. Data statistics were carried out on the application results in a certain sheep farm in this area. The results showed that the clinical application of the traditional Chinese medicine composition in treating lamb diarrhea had excellent effects. The clinical recovery rate of the demonstration group was 100% after 3 days, and the body weight increased to a certain extent (the difference was not significant).

[0096] Table 7 Application of the traditional Chinese medicine composition in treating damp-cold diarrhea of sheep in a certain sheep farm in this area

[0097]

[0098] 4. Application results in Gaogang Base

[0099] During the period from March to May 2023, the traditional Chinese medicine composition was clinically applied to treat meat sheep diarrhea in 6 sheep farms (Table 8). A total of 58 diarrheal meat sheep aged 4 - 5 months were treated, and 56 sheep recovered clinically (clinical recovery rate 96.55%), while 2 sheep showed no clinical effect; the average number of medication times was 4.52 times, and the average recovery time was 2.16 days. The clinical application had excellent effects. Data statistics were carried out on the application results in a certain ecological agriculture co., ltd. in this area. The results showed that the traditional Chinese medicine composition could treat the diarrhea of adult meat sheep (Hu sheep). The clinical recovery rate of the traditional Chinese medicine treatment group was 100% after 3 days, and the body weight increased to a certain extent (the difference was not significant).

[0100] Table 8 Application of the traditional Chinese medicine composition in treating damp-cold diarrhea of sheep in a certain original ecological agriculture co., ltd. in this area

[0101]

[0102] 5. Summary

[0103] The above data indicate that the clinical application of the traditional Chinese medicine composition has the characteristics of high recovery rate, short medication time, and wide application range (it has significant effects on sheep at different developmental stages), can effectively treat damp-cold diarrhea of sheep, verifies the application effect of the traditional Chinese medicine composition in clinical trials, and shows that the traditional Chinese medicine composition can be applied to damp-cold diarrhea of sheep.

[0104] Example 3

[0105] Integrated demonstration of the traditional Chinese medicine composition in treating damp-cold diarrhea of sheep

[0106] In this example, the traditional Chinese medicine composition was demonstrated and applied in 22 sheep farms at 5 promotion and demonstration bases in Haimen, Rugao, Gaogang, Suining, and Xuzhou (Table 9); a total of 655 meat sheep with diarrhea were treated, among which 504 recovered clinically, and the overall clinical recovery rate reached 91.45%. The average number of medication times was 5.976, and the average recovery time was 3.45 days.

[0107] Table 9 Demonstration application of traditional Chinese medicine composition in the treatment of damp-cold diarrhea in sheep at the promotion and demonstration bases

[0108]

[0109] The above data indicate that the treatment method of traditional Chinese medicine composition has good practicability and effect. This integrated demonstration verified the high efficiency and feasibility of traditional Chinese medicine in treating diarrhea in meat sheep, providing a scientific prevention and control plan for farms.

[0110] Therefore, compared with anti-infective drugs in modern medicine, traditional Chinese medicine mainly acts on the animal body, improving the internal environment of the body through the properties and flavors of the drugs (cold, heat, warm, cool; sour, bitter, sweet, pungent, salty) to achieve the purpose of yin-yang balance and maintain the normal operation of qi, blood, and body fluids in the body. For example, for damp-cold diarrhea, drugs with the effects of warming yang to dispel cold, drying dampness and promoting qi movement, strengthening the spleen and replenishing qi, and astringing diarrhea can be used for treatment. Modern medicine has also proven that traditional Chinese medicine has multiple functions such as regulating the intestinal flora, exerting anti-inflammatory and antioxidant effects, enhancing the body's immunity, and improving the gastrointestinal digestion and absorption function; in addition, under the guidance of the theory of syndrome differentiation and treatment, traditional Chinese medicine has the advantages of being natural, environmentally friendly, having small side effects, comprehensive curative effects, and being less likely to produce drug resistance.

Claims

1. A Chinese medicine composition for treating diarrhea caused by cold and dampness in sheep, characterized in that: The mass components of the traditional Chinese medicine composition are as follows: 13-16 parts of dried ginger, 5-8 parts of yuyuliang, 4-8 parts of costusroot, 8-12 parts of honeysuckle, 5-7 parts of tangerine peel, 4-8 parts of atractylodes, 5-7 parts of betel nut, 9-11 parts of astragalus and 5-7 parts of roasted liquorice.

2. A Chinese medicine composition for treating sheep diarrhea caused by cold and dampness according to claim 1, characterized in that: The mass components of the traditional Chinese medicine composition are as follows: 15 parts of dried ginger, 6 parts of yuyuliang, 6 parts of costusroot, 10 parts of honeysuckle, 6 parts of tangerine peel, 6 parts of atractylodes, 6 parts of betel nut, 10 parts of astragalus and 6 parts of roasted liquorice.

3. A method for preparing a Chinese medicine composition for treating sheep diarrhea caused by cold and dampness as claimed in any one of claims 1 to 2, characterized in that: The preparation steps are as follows: Step (1): adding 3 times the volume of distilled water to the raw material to be boiled according to the proportion and soaking them; Step (2): After the soaking is completed, the soaked raw materials are boiled over high heat, and then the boiled raw materials are decocted over low heat. After the decocting is completed, the medicinal residues are filtered through an 80-mesh filter sieve and the medicinal liquid is collected. The filtered medicinal residues are decocted again; Step (3): Add 3 times the volume of distilled water to the filtered residue, boil it over high heat, then continue to decoct it over low heat. After decoction is completed, filter the residue with an 80-mesh filter sieve and discard it, collect the liquid medicine, combine the two decoctions, and concentrate them over low heat to the same volume as the raw materials to prepare the final water decoction.

4. The method for preparing a Chinese medicine composition for treating sheep diarrhea caused by cold and dampness according to claim 3, characterized in that: In step (1), the soaking time is 1 hour; In step (2), the time for decocting on low heat is: 2 hours; In step (3), the time for continuing decocting over low heat is 2 hours.

5. Use of a Chinese medicine composition for treating sheep diarrhea caused by cold and dampness prepared by the preparation method according to claim 3 in preparing a drug for improving perianal feces contamination in diarrheal sheep.

6. Use of a traditional Chinese medicine composition for treating sheep diarrhea caused by cold and dampness prepared by the preparation method according to claim 3 in preparing a drug for reducing the diarrhea rate of diarrheal sheep.

7. Use of a traditional Chinese medicine composition for treating sheep diarrhea caused by cold and dampness prepared by the preparation method according to claim 3 in preparing a drug for reducing the diarrhea index of diarrheal sheep.

8. The use according to claim 7, characterized in that: The diarrhea index refers to: observing the feces of the test sheep every day and scoring them, granular feces are recorded as 1 point; soft feces that can be formed are recorded as 2 points; thick feces, unformed, and no fecal and water separation are recorded as 3 points; liquid feces, unformed, and fecal and water separation are recorded as 4 points. A feces score of >2 points is considered diarrhea, and ≤2 points is cured.

9. Use of a traditional Chinese medicine composition for treating sheep diarrhea caused by cold and dampness prepared by the preparation method according to claim 3 in preparing a drug for improving abnormal blood routine indicators in sheep with diarrhea.

10. Use of a traditional Chinese medicine composition for treating sheep diarrhea caused by cold and dampness prepared by the preparation method according to claim 3 in preparing a drug for improving abnormal blood biochemical indicators in diarrheal sheep.

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