Fertilizer composition containing ascophyllum nodosum extract

Abstract

The invention belongs to the technical field of fertilizers, and particularly relates to a fertilizer composition containing ascophyllum nodosum extract. The invention discloses a fertilizer composition containing ascophyllum nodosum extract, the core component of the fertilizer composition is composed of the ascophyllum nodosum extract and fulvic acid, the mass ratio of the ascophyllum nodosum extract to the fulvic acid is (1-40): (1-20), and the ascophyllum nodosum extract and the fulvic acid have a synergistic effect. Field test results show that the fertilizer containing the ascophyllum nodosum extract and fulvic acid has the effects of promoting plant growth and increasing yield, and has the effect of reducing soil salinity and alkalinity. The invention provides a new technical path for saline-alkali soil improvement and plant growth promotion.

Description

Technical Field

[0001] The present invention belongs to the technical field of fertilizers, and particularly relates to a fertilizer composition containing Ascophyllum nodosum extract. Background Art

[0002] With global climate change and unreasonable land use, the area of saline-alkali soil is gradually expanding, seriously affecting the growth and yield of crops. Saline-alkali land includes both primary saline-alkali land and secondary saline-alkali land. Primary saline-alkali land is mainly distributed in the arid and semi-arid northwest regions of our country and around the rivers near coastal areas. Secondary saline-alkali land is mainly in vegetable greenhouses that have been cultivated for many years. Due to the unreasonable use of chemical fertilizers year after year, salt accumulates on the soil surface. When the soil salt content exceeds 0.1%, it will begin to inhibit the growth of crops.

[0003] Currently, the commonly used methods for improving saline-alkali land in the market include using strongly acidic fertilizers to neutralize the alkalinity of the soil, mineral source fulvic acid, or water-soluble fertilizers containing humic acid, and fertilizers containing microbial agents. These methods can improve the soil environment to a certain extent, but the effect is slow. Using strongly acidic fertilizers may cause certain damage to the soil structure. Therefore, there is an urgent need for an efficient and environmentally friendly fertilizer to improve the quality of saline-alkali soil and promote the growth of crops.

[0004] Seaweeds are rich in various active substances and nutrients, including algal polysaccharides, fucoxanthin, phlorotannins, betaine, plant hormones, phenolic compounds, terpenoid compounds, mineral elements, crude protein, and crude fiber, etc. These substances have extremely strong growth-promoting and stress-resistant effects on crops and are an important supplement to terrestrial resources. In particular, active substances such as alginate, fucoidan, and fucoxanthin in seaweeds have significant effects on improving the soil environment and promoting plant growth.

[0005] In view of the advantages of seaweeds in improving the soil environment and promoting crop growth, it is of great significance to develop a fertilizer composition containing seaweed extract for reducing soil salinity and promoting growth. Summary of the Invention

[0006] To solve the above technical problems, the present invention screens different algal extracts, aiming to obtain algal extracts with significant effects on promoting plant growth and improving soil structure, and screens components that have a synergistic effect when compounded with them to form a fertilizer composition containing algal extract for soil environment improvement and plant growth promotion.

[0007] On the one hand, the present invention provides a fertilizer composition containing Ascophyllum nodosum extract, and the core components of the fertilizer composition are composed of Ascophyllum nodosum extract and fulvic acid.

[0008] Further, in the fertilizer composition containing Ascophyllum nodosum extract, the mass ratio of the Ascophyllum nodosum extract to fulvic acid is 1 - 40:1 - 20.

[0009] Further, in the fertilizer composition containing the Ascophyllum nodosum extract, the mass ratio of the Ascophyllum nodosum extract to fulvic acid is 10 - 30:3 - 12.

[0010] Further, in the fertilizer composition containing the Ascophyllum nodosum extract, the mass ratio of the Ascophyllum nodosum extract to fulvic acid is 20:9.

[0011] On the other hand, the present invention also provides a fertilizer containing the fertilizer composition containing the Ascophyllum nodosum extract.

[0012] Further, the components of the fertilizer include calcium lignosulfonate.

[0013] On yet another aspect, the present invention also provides the application of the fertilizer in promoting plant growth.

[0014] Further, the present invention provides the application of the fertilizer in promoting the growth of cotton or soybeans.

[0015] Further, the present invention provides the application of the fertilizer in increasing the cotton yield.

[0016] In addition, the present invention also provides the application of the fertilizer in improving the soil environment, and the improvement of the soil environment is to reduce the soil salinity.

[0017] Compared with the prior art, the technical solution provided by the present invention has at least the following beneficial effects or advantages:

[0018] The present invention obtains the Ascophyllum nodosum extract by screening different algal extracts, and further mixes the Ascophyllum nodosum extract to find that the mass ratio of the Ascophyllum nodosum extract to fulvic acid in the range of 1 - 40:1 - 20 has a better effect on promoting the growth of soybeans than the single agent, indicating that the mixture of the two has a synergistic effect on promoting the growth of soybeans. When the mass ratio of the Ascophyllum nodosum extract to fulvic acid is in the range of 10 - 30:3 - 12, the synergistic effect of the mixture of the two on promoting the growth of soybeans is more significant. When the mass ratio of the Ascophyllum nodosum extract to fulvic acid is 20:9, the effect of the mixture of the two on promoting the growth of soybeans is the most significant.

[0019] Field test results show that the fertilizer containing the Ascophyllum nodosum extract and fulvic acid, when the mass ratio of the mixture is in the range of 1 - 40:1 - 20, compared with the control, increases the number of cotton bolls and the cotton yield. Especially when the mass ratio of the Ascophyllum nodosum extract to fulvic acid in the fertilizer is 20:9, the cotton yield increases by 97.13 compared with the blank control, indicating that the fertilizer containing the Ascophyllum nodosum extract and fulvic acid can significantly increase the cotton yield and has a significant effect on promoting the growth of cotton.

[0020] In addition, the results of field trials showed that the soil EC value decreased after applying the fertilizer containing Ascophyllum nodosum extract and fulvic acid, compared with that before application. It is worth noting that the soil EC value in the blank control group increased compared with the EC value before application, indicating that the fertilizer composition containing Ascophyllum nodosum extract and fulvic acid has the effect of reducing soil salinity. Detailed implementation mode

[0021] Next, the technical solutions of the present invention will be described in conjunction with embodiments. However, the present invention is not limited to the following embodiments.

[0022] In order to enable those skilled in the art to better understand and implement the technical solutions of the present invention, the present invention will be further described below in conjunction with specific embodiments, but the exemplified embodiments are not intended to limit the present invention.

[0023] In the following embodiments, the experimental methods and detection methods are all conventional methods unless otherwise specified; the reagents and materials can be purchased on the market unless otherwise specified.

[0024] The Ascophyllum nodosum extract was purchased from Shaanxi Snow Biotechnology Co., Ltd. and was in the form of a brownish-yellow powder.

[0025] The Sargassum extract was purchased from Shaanxi Snow Biotechnology Co., Ltd. and was in the form of a brownish-yellow powder.

[0026] The green algae extract was purchased from Shaanxi Snow Biotechnology Co., Ltd. and was in the form of a green powder.

[0027] Example 1

[0028] This example was a test on the synergistic growth-promoting effect of Ascophyllum nodosum extract and fulvic acid.

[0029] Treatment of test reagents: The test reagents, Ascophyllum nodosum extract and fulvic acid, were diluted with water respectively to prepare single-agent mother liquors and mixed-agent mother liquors with various ratios. Different mass concentration gradients were set for the single agents and the mixed agents with ratios, and they were reserved for use.

[0030] Test soybean variety: Xin soybean No. 27.

[0031] Test time and location: The test was carried out at Northwest A&F University from 2022 to 2023.

[0032] The above-mentioned mixed agents and single-agent mother liquors with different ratios and concentrations were diluted with clear water at a ratio of 1:300 and reserved for use.

[0033] The test set a clear water group as the blank control group (CK group), a single-agent group of test reagents, and a mixed-agent group, that is, the test group of the composition of Ascophyllum nodosum extract and fulvic acid.

[0034] Soybean seeds were sown in plastic nutrient boxes, with 50 seeds per box. On the day of sowing the soybean seeds, an extract of Ascophyllum nodosum and fulvic acid single agents and a mixture diluted at 1:300 were applied at 30 mL / box as fertilizers. Each treatment had 3 replicates, and the group treated with clear water was set as the blank control group (CK group). The seedlings were tested in a light incubator at 25°C / 28°C (night / day). On the 15th day, plant height and root length were measured, and the root length growth rate and plant height growth rate were calculated. The Gowing method was used to evaluate the growth-promoting performance of each effective component after mixing on soybeans. The formula is E 0 = X + [Y × (100 - X) / 100], E 0 represents the theoretical root length growth rate and theoretical plant height growth rate of soybeans in the composition (A + B) when the dosage is (P + Q). X represents the root length growth rate and plant height growth rate of soybeans treated with agent A when the dosage is P, and Y represents the root length and plant height of soybeans treated with agent B when the dosage is Q; E represents the actual root length growth rate and plant height growth rate of soybeans treated with the composition.

[0035] When E - E 0 > 10% is a synergistic effect; when E - E 0 < - 10% is an antagonistic effect; when the value of E - E 0 is between ±10% is an additive effect.

[0036] Root length growth rate (%) = (root length of the experimental group - root length of the blank control group) / root length of the blank control group × 100

[0037] Plant height growth rate (%) = (plant height of the experimental group - plant height of the blank control group) / plant height of the blank control group × 100

[0038] The test results of the effects of different treatments on the plant height of soybeans are shown in Table 1, and the test results of the effects of different treatments on the root length of soybeans are shown in Table 2.

[0039] Table 1 Test results of the effects of Ascophyllum nodosum extract and fulvic acid composition on the plant height of soybeans

[0040]

[0041]

[0042] Table 2 Test results of the effects of Ascophyllum nodosum extract and fulvic acid composition on the root length of soybeans

[0043]

[0044]

[0045] The results in Table 1 and Table 2 show that within the range of the mixing mass ratio of Ascophyllum nodosum extract to fulvic acid from 1 - 40:1 - 20, the growth - promoting effect of the mixture of Ascophyllum nodosum extract and fulvic acid on the plant height and root length of soybeans is better than that of the single agent, indicating that the mixture of Ascophyllum nodosum extract and fulvic acid has a synergistic effect on the growth promotion of soybeans. When the mixing mass ratio of Ascophyllum nodosum extract to fulvic acid is in other ranges, the two show an additive effect; within the range of 10 - 30:3 - 12 of the mixing mass ratio of Ascophyllum nodosum extract to fulvic acid, the synergistic effect is more significant; when the mixing mass ratio of Ascophyllum nodosum extract to fulvic acid is 20:9, the synergistic effect is the best.

[0046] Example 2

[0047] This example is a fertilizer containing a fertilizer composition of Ascophyllum nodosum extract and fulvic acid.

[0048] A fertilizer containing a fertilizer composition of Ascophyllum nodosum extract and fulvic acid, including 1%

[0049] of Ascophyllum nodosum extract, 20% of fulvic acid, 1% of zinc sulfate, and 10% of calcium lignosulfonate by mass percentage. Marked as Fertilizer 1#.

[0050] Example 3

[0051] This example is a fertilizer containing a fertilizer composition of Ascophyllum nodosum extract and fulvic acid.

[0052] A fertilizer containing a fertilizer composition of Ascophyllum nodosum extract and fulvic acid, including 5% of Ascophyllum nodosum extract, 15% of fulvic acid, 2% of zinc sulfate, and 12% of calcium lignosulfonate by mass percentage. Marked as Fertilizer 2#.

[0053] Example 4

[0054] This example is a fertilizer containing a fertilizer composition of Ascophyllum nodosum extract and fulvic acid.

[0055] A fertilizer containing a fertilizer composition of Ascophyllum nodosum extract and fulvic acid, including 10% of Ascophyllum nodosum extract, 12% of fulvic acid, 1.5% of zinc sulfate, and 15% of calcium lignosulfonate by mass percentage. Marked as Fertilizer 3#.

[0056] Example 5

[0057] This example is a fertilizer containing a fertilizer composition of Ascophyllum nodosum extract and fulvic acid.

[0058] A fertilizer containing a fertilizer composition of Ascophyllum nodosum extract and fulvic acid, including 20% of Ascophyllum nodosum extract, 9% of fulvic acid, 2% of zinc sulfate, and 17% of calcium lignosulfonate by mass percentage. Marked as Fertilizer 4#.

[0059] Example 6

[0060] This example is a fertilizer of a fertilizer composition containing Ascophyllum nodosum extract and fulvic acid.

[0061] A fertilizer of a fertilizer composition containing Ascophyllum nodosum extract and fulvic acid, including 25% of Ascophyllum nodosum extract, 6% of fulvic acid, 2% of zinc sulfate, and 20% of calcium lignosulfonate by mass percentage. Marked as Fertilizer 5#.

[0062] Example 7

[0063] This example is a fertilizer of a fertilizer composition containing Ascophyllum nodosum extract and fulvic acid.

[0064] A fertilizer of a fertilizer composition containing Ascophyllum nodosum extract and fulvic acid, including 30% of Ascophyllum nodosum extract, 3% of fulvic acid, 2% of zinc sulfate, and 20% of calcium lignosulfonate by mass percentage. Marked as Fertilizer 6#.

[0065] Example 8

[0066] This example is a fertilizer of a fertilizer composition containing Ascophyllum nodosum extract and fulvic acid.

[0067] A fertilizer of a fertilizer composition containing Ascophyllum nodosum extract and fulvic acid, including 40% of Ascophyllum nodosum extract, 1% of fulvic acid, 2% of zinc sulfate, and 20% of calcium lignosulfonate by mass percentage. Marked as Fertilizer 7#.

[0068] Control 1

[0069] Control 1 is a fertilizer composition containing Sargassum extract.

[0070] A fertilizer composition containing Sargassum extract and fulvic acid, including 20% of Sargassum extract, 9% of fulvic acid, 2% of zinc sulfate, and 20% of calcium lignosulfonate by mass percentage. Marked as Control 1#.

[0071] Control 2

[0072] Control 2 is a fertilizer composition containing green algae extract.

[0073] A fertilizer composition containing green algae extract and fulvic acid, including 20% of green algae extract, 9% of fulvic acid, 2% of zinc sulfate, and 20% of calcium lignosulfonate by mass percentage. Marked as Control 2#.

[0074] Example 9

[0075] This example is a field fertilizer efficiency test of a fertilizer of a fertilizer composition containing Ascophyllum nodosum extract and fulvic acid.

[0076] Field test site: The field fertilizer efficiency test of the fertilizer containing the extract of Ascophyllum nodosum and fulvic acid was carried out in the cotton planting area of Kashgar, Xinjiang.

[0077] Cotton variety for test: Zhaofeng No. 28.

[0078] The total test area is 10 mu, which is evenly divided into 10 test areas, each test area is 1 mu, and 3 replicates are set in each test area. Before cotton planting, basal fertilizer was applied conventionally. 10 days after applying basal fertilizer, soil samples from 10 test areas were collected to measure the soil EC value, and the soil EC value was recorded. During the cotton seedling stage, fertilizers 1# - 7# of Examples 2 - 8 (Test Group 1 - Test Group 7), contrast agent 1# (CK1), contrast agent 2# (CK2) and clear water (blank control KC) were applied. The application method was drip irrigation. Drip irrigation was carried out continuously 2 times during the seedling stage. The first drip irrigation was when the cotton plant height reached 15 cm, and the second drip irrigation was when the cotton plant height reached 40 - 50 cm. The dosage of both drip irrigations was 1 L / mu. During the cotton maturity stage, the number of cotton bolls, single boll weight and cotton yield data of cotton were recorded to evaluate the effects of different treatments on cotton growth and its yield. After cotton harvest, soil samples were collected to measure the soil EC value to evaluate the effects of each treatment on the soil ecological structure.

[0079] Table 3 Test results of the effects of different treatments on soil EC value

[0080] Test Grouping Soil EC Value before Application Soil EC Value after Cotton Harvest Change in Soil EC Value Fertilizer 1# 2.72 2.21 -0.51 Fertilizer 2# 2.73 2.23 -0.5 Fertilizer 3# 2.71 2.25 -0.46 Fertilizer 4# 2.75 2.24 -0.51 Fertilizer 5# 2.72 2.21 -0.51 Fertilizer 6# 2.73 2.23 -0.5 Fertilizer 7# 2.76 2.26 -0.5 Control Agent 1# (CK1) 2.71 2.22 -0.49 Control Agent 2# (CK2) 2.73 2.23 -0.5 Blank Control (KC) 2.76 3.44 0.68

[0081] The results of the effects of applying fertilizers containing different fertilizer compositions of Ascophyllum nodosum extract and fulvic acid on the soil ecological structure of the test field are shown in Table 3. Before applying the fertilizers containing Ascophyllum nodosum extract and fulvic acid and the contrast agent fertilizers, the EC values of the soil in each area of the test field were between 2.71 and 2.76, with no significant difference. During the field test, the fertilizers containing Ascophyllum nodosum extract and fulvic acid and the contrast agent fertilizers were drip-irrigated 2 times. The test results of the soil EC value in the test area after cotton harvest showed that compared with the blank control group, the EC values decreased after applying fertilizers 1# - 7#, contrast agent 1# and 2#. Only the soil EC value of the blank control group increased after cotton harvest compared with that before application, indicating that applying fertilizers 1# - 7# and the contrast agent fertilizers can reduce the soil EC value, and the fertilizer containing the fertilizer composition of Ascophyllum nodosum extract and fulvic acid has the effect of reducing soil salinity.

[0082] Table 4 Test results of the effects of different treatments on cotton growth promotion

[0083] Test Grouping Number of Cotton Bolls per Meter Single Boll Weight (g) Yield (kg) Yield Increase Rate (%) Fertilizer 1# 121 6.73 375.72 33.53 Fertilizer 2# 137 6.85 432.14 53.58 Fertilizer 3# 151 7.01 489.36 73.91 Fertilizer 4# 165 7.25 554.69 97.13 Fertilizer 5# 147 6.98 471.27 67.49 Fertilizer 6# 135 6.72 414.22 47.21 Fertilizer 7# 118 6.61 380.51 35.23 Control Agent 1# (CK1) 105 6.41 311.95 10.86 Control Agent 2# (CK2) 108 6.38 306.41 8.90 Blank Control (KC) 102 6.01 281.38 -

[0084] The test results of the effects of fertilizers applying different fertilizer compositions containing Ascophyllum nodosum extract and fulvic acid on cotton growth promotion are shown in Table 4. Comparing with the blank control, applying fertilizers 1#-7#, contrast agent 1# and contrast agent 2# significantly increased cotton yield. The yield increase ranged from 33.53% to 97.13% compared with the blank control. Especially, the yield increase of fertilizer 4# was as high as 97.13%. The yield increase of contrast agent 1# and contrast agent 2# compared with the blank control was 10.86% and 8.9% respectively, and the yield increase effects were not as good as those of applying fertilizers 1#-7#, indicating that the mixed application of Sargassum extract and fulvic acid and the mixed application of green algae extract and fulvic acid in the contrast agent had limited effects on cotton growth promotion compared with Ascophyllum nodosum extract, and the growth promotion effect was far less than that of the mixed application of Ascophyllum nodosum extract and fulvic acid. It shows that the fertilizer containing the fertilizer composition of Ascophyllum nodosum extract and fulvic acid has a significant effect on cotton growth promotion, and fertilizer 4# containing the fertilizer composition of Ascophyllum nodosum extract and fulvic acid is the best embodiment of the present invention.

[0085] As described above, the basic principles, main features and advantages of the present invention are preferably described. The above embodiments and the description are only for describing the preferred embodiments of the present invention. The present invention is not limited by the above embodiments. Without departing from the spirit and scope of the present invention, various changes and improvements made by those of ordinary skill in the art to the technical solutions of the present invention shall fall within the protection scope determined by the present invention.

Claims

1. A fertilizer composition containing an Ascophyllum nodosum extract, characterized in that: The core components of the fertilizer composition are composed of Ascophyllum nodosum extract and fulvic acid.

2. The fertilizer composition containing the Ascophyllum nodosum extract according to claim 1, characterized in that The mass ratio of the Ascophyllum nodosum extract to fulvic acid is 1-40:1-20.

3. The fertilizer composition containing the Ascophyllum nodosum extract according to claim 2, characterized in that: The mass ratio of the Ascophyllum nodosum extract to fulvic acid is 10-30:3-12.

4. The fertilizer composition containing the Ascophyllum nodosum extract according to claim 3, characterized in that The mass ratio of the Ascophyllum nodosum extract to fulvic acid is 20:

9.

5. A fertilizer comprising the fertilizer composition containing the Ascophyllum nodosum extract according to any one of claims 1 to 4.

6. The fertilizer according to claim 5, characterized in that The components of the fertilizer include calcium lignin sulfonate.

7. Use of the fertilizer according to claim 5 or 6 in promoting plant growth.

8. The use according to claim 7, wherein the plant is cotton or soybean.

9. The use according to claim 8, characterized in that: The cotton growth promotion also includes increasing cotton yield.

10. Use of the fertilizer according to claim 6 in improving soil environment, characterized in that: The improving soil environment is to reduce soil salinity.

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