Device and method for accelerating microbial mutation based on ray irradiation of electron accelerator

Abstract

The invention discloses a device and method for accelerating microbial mutation based on ray irradiation of an electron accelerator, and belongs to the field of mutation devices.The device comprises a mutation box, the electron accelerator is fixedly connected to the upper portion of the mutation box, a radiation window is fixedly connected to the lower portion of the electron accelerator, and a ventilation assembly is movably installed in the mutation box; through the arranged ventilation assembly, temperature and humidity can be conveniently provided for the mutation box, a culture dish can be conveniently kept in a ventilation environment with appropriate temperature and humidity, the microbial culture effect is better, through reciprocating movement of the arranged heated dredging assembly, movable rods and movable plates, airflow is intermittently discharged, fan blades are blown, and the ventilation effect is improved. The culture table rotates slowly, the culture dishes are heated evenly and make more sufficient contact with moisture, the brush continuously brushes the filter plate, air enters the filter plate smoothly, and the filter plate is not prone to being blocked. The airflow exhausted by the exhaust cylinder is conveniently introduced again through the air collecting cover and the air inducing pipe, so that the temperature rise of the mutation box is quicker.

Description

Technical Field

[0001] The present invention relates to the field of mutation devices, and particularly to a device and method for accelerating microbial mutation based on electron accelerator ray irradiation. Background Art

[0002] Irradiation with high-energy electron beams generated by electron accelerators can cause physical, chemical, and biological effects in some substances, and can effectively kill germs, viruses, and pests. This technology has been widely applied in material modification, new material production, environmental protection, processing production, sterilization and disinfection of medical and health supplies, and sterilization and preservation of food in industrial production. In the cultivation of microorganisms, the probability of microbial mutation can be effectively increased through the ray irradiation of an electron accelerator. In the prior art, a Chinese utility model patent with the publication number CN212907120U discloses an electron accelerator irradiation device. This utility model preliminarily positions the cargo box through a roller group, cleans the dust on the outside of the cargo box through the air inlet and outlet, reduces the escape of radiation through the design of the shielding inner shell, and re-positions and flips the cargo box through the rotating disk and the second electric telescopic rod to irradiate multiple sides of the cargo box reliably, improving the irradiation efficiency of the goods.

[0003] In the cultivation of microorganisms, mutagenic cultivation can also be carried out through an electron accelerator. Since a certain temperature needs to be provided for the cultivation environment during the cultivation of microorganisms, and the humidity and ventilation conditions in the cultivation environment need to be maintained in a certain state, although this utility model can place the culture dish under the electron accelerator, due to the inability to provide the necessary temperature, humidity, and ventilation conditions, it is easy to reduce the efficiency of mutagenic cultivation. Therefore, a device for accelerating microbial mutation based on electron accelerator ray irradiation is proposed. Summary of the Invention

[0004] The main object of the present invention is to provide a device and method for accelerating microbial mutation based on electron accelerator ray irradiation, which can effectively solve the problems in the background art.

[0005] To achieve the above object, the technical solution adopted by the present invention is as follows:

[0006] Device for accelerating microbial mutation by electron accelerator ray irradiation, comprising a mutation chamber, an electron accelerator is fixedly connected to the upper position of the mutation chamber, a radiation window is fixedly connected to the lower position of the electron accelerator, a ventilation component is movably installed in the mutation chamber, the ventilation component includes an exhaust fan, an air inlet pipe and a filter plate, the exhaust fan is fixedly connected to the lower position of the mutation chamber, the air inlet pipe is fixedly connected to the bottom surface of the mutation chamber, the filter plate is fixedly connected to the lower end of the air inlet pipe, a heat-dissipating and dredging component is movably installed on the mutation chamber, the heat-dissipating and dredging component includes a culture table, fan blades, a first screw rod, an arc-shaped sealing plate, an air outlet, a movable rod, a second sealing gasket, a transmission plate, a first runner, a second runner and a brush, the culture table is movably connected in the mutation chamber, the first screw rod is movably installed with the arc-shaped sealing plate, the air outlet is opened at one side position of the exhaust duct, the movable rod is movably installed in the exhaust duct, one end of the movable rod is fixedly connected with the second sealing gasket, and the other end is movably installed with the first runner through the transmission plate, the second runner is movably installed on the side position of the first runner, and the brush is fixedly connected above the second runner.

[0007] Preferably, a box door is movably connected to the front position of the mutation chamber, a bottom plate is fixedly connected to the lower position of the mutation chamber, a mounting frame is fixedly connected to the bottom plate, the electron accelerator is fixedly connected to the mounting frame, and the bottom plate is fixedly connected to the mutation chamber through support feet.

[0008] Preferably, the ventilation component further includes an electric heating plate, an exhaust pipe, an exhaust duct, a mist chamber, an atomizing nozzle and a water inlet pipe, the exhaust pipe is fixedly connected to the bottom surface position of the mutation chamber, the air suction port of the exhaust fan is fixedly communicated with the exhaust pipe, and the exhaust port of the exhaust fan is fixedly communicated with the exhaust duct, the electric heating plate is fixedly connected to the inner wall of the mutation chamber, the mist chamber is fixedly connected to the side position of the air inlet pipe, one end of the water inlet pipe penetrates through the mist chamber and is fixedly connected with the atomizing nozzle, and a solenoid valve is movably connected to the water inlet pipe.

[0009] Preferably, the ventilation component further includes a PLC controller and a temperature and humidity sensor, the PLC controller is fixedly connected to the mounting frame, and the temperature and humidity sensor is fixedly connected to the mutation chamber.

[0010] Preferably, the heat-dissipating and dredging component further includes a culture tank, a first rotating rod and a first bearing, the culture tank is opened on the culture table, the first rotating rod is movably installed in the mutation chamber through the first bearing, and the upper end and the lower end of the first rotating rod are respectively fixedly connected to the culture table and the fan blades.

[0011] Preferably, the heat-removing and dredging component further includes a first connecting plate, a second connecting plate, a second bearing, a connecting head, a handle, a first gasket, and an exhaust hole. The first connecting plate and the second connecting plate are fixedly connected to the side of the exhaust cylinder. The arc-shaped sealing plate is movably installed at the side of the exhaust cylinder. The first screw rod is movably installed with the first connecting plate and the second connecting plate through the second bearing respectively. The connecting head is fixedly connected to the side of the arc-shaped sealing plate, and the connecting head is movably installed with the first screw rod through a threaded hole. The first gasket is fixedly connected to the inner side of the arc-shaped sealing plate. The handle is fixedly connected to one end of the first screw rod. The exhaust hole is opened on the exhaust cylinder, and the arc-shaped sealing plate is located at the side of the exhaust hole.

[0012] Preferably, the heat-removing and dredging component further includes a movable plate, a spring, and a rotating shaft. The movable plate is fixedly connected to one end of the movable rod, and a second gasket is fixedly connected to the movable plate. The spring is movably installed on the movable rod and is located inside the exhaust cylinder. The spring is located at the side of the movable plate. Both ends of the transmission plate are movably installed with the movable rod and the first runner through the rotating shaft respectively.

[0013] Preferably, the heat-removing and dredging component further includes a second rotating rod, a third bearing, a third rotating rod, a fourth bearing, and a rotating belt. The upper ends of the second rotating rod and the third rotating rod are movably installed with the bottom surface of the mutation box through the third bearing and the fourth bearing respectively. The lower ends of the second rotating rod and the third rotating rod are fixedly connected to the first runner and the second runner respectively. The first runner is movably installed with the second runner through the rotating belt.

[0014] Preferably, the heat-removing and dredging component further includes an air collecting hood and an air guiding pipe. The air collecting hood is fixedly communicated with the air guiding pipe. A through hole is provided on the air collecting hood corresponding to the position of the first rotating rod, and the fan blade is movably installed inside the air collecting hood. The air outlet of the air guiding pipe is located below the filter plate.

[0015] A method for accelerating microbial mutation based on electron accelerator ray irradiation includes the following steps:

[0016] S1: A culture dish is placed in the culture tank on the culture table. After closing the box door, the temperature and humidity inside the mutation box are observed through the temperature and humidity sensor. The PLC controller controls the electric heating plate to start to provide heat for microbial culture. The PLC controller controls the exhaust fan to start, so that the exhaust fan sucks the gas inside the mutation box, causing a negative pressure inside the mutation box, and sucking the external gas through the air inlet pipe to provide ventilation conditions for microbial culture. The PLC controller controls the electron accelerator to start, and irradiates the rays through the radiation window to the culture dish below for radiation mutagenesis.

[0017] S2: The intake air duct filters the external air flow through the filter plate at the lower end, intercepting dust at an external position. The temperature and humidity sensor detects the moisture in the mutation chamber. When it is necessary to increase the moisture concentration, the PLC controller controls the solenoid valve on the water inlet pipe to open. The water inlet pipe is connected to an external water pipe, and the liquid water is turned into mist by the atomizing nozzle and enters the mutation chamber along with the air flow, thereby changing the moisture concentration in the mutation chamber;

[0018] S3: The exhaust fan discharges the air flow in the mutation chamber into the exhaust duct, causing the second gasket and the movable plate to move sideways under the push of the air flow pressure. The movable rod moves synchronously, drives the first runner to rotate through the transmission plate, and the first runner drives the second runner to rotate through the rotating belt, so that the brush on the second runner rotates synchronously, and the filter plate is brushed by the brush to remove dust, avoiding blockage of the filter plate and causing poor air intake;

[0019] S4: During the process of the movable plate and the movable rod moving sideways, when the movable plate moves to the side position of the air outlet, the air flow is discharged from the air outlet, blowing against the fan blades, causing the fan blades to rotate intermittently. The culture platform rotates actively in the mutation chamber, making the culture dish receive uniform heat and come into more sufficient contact with the water vapor in the air flow;

[0020] S5: The fan blades rotate actively in the air collecting hood, causing part of the air flow to pass through the air guiding pipe and be re-introduced to the lower position of the filter plate, so that the air flow containing heat can be recovered and utilized, facilitating the rapid heating of the temperature in the mutation chamber.

[0021] Compared with the prior art, the present invention has the following beneficial effects:

[0022] In the present invention, through the ventilation component provided, the temperature and humidity sensor monitors the temperature and humidity conditions in the mutation chamber, and then starts the electric heating plate to heat the mutation chamber, providing part of the heat. The exhaust fan extracts the gas in the mutation chamber outwards, making the air flow in the mutation chamber flow and circulate with the external air flow. The atomizing nozzle provides atomized water vapor for the air flow entering the mutation chamber, so that the mutation chamber is easy to maintain a warm and humidity-adapted culture environment, facilitating better mutagenesis and culture of microorganisms;

[0023] In the present invention, through the heat receiving and dredging component provided, during the exhaust process of the exhaust fan, the movable plate and the movable rod are reciprocally moved by the air flow, causing the air flow to be discharged intermittently, pushing the fan blades, making the culture platform rotate, facilitating the culture dish to receive uniform heat, and also being able to come into sufficient contact with the moisture, making the heat and moisture levels of the culture dish uniform. The movable rod drives the first runner to rotate, causing the first runner to drive the second runner to rotate, and the brush continuously brushes the filter plate, making it easy to remove the dust on the filter plate and not easy to be blocked, thereby maintaining smooth air intake;

[0024] In the present invention, through the provided heat-receiving and dredging component, the air flow discharged from the exhaust duct is introduced below the filter plate through the air collecting hood and the air guiding pipe, facilitating the air flow containing heat to flow back into the mutation box again, avoiding heat waste, and enabling the mutation box to heat up more quickly. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 is a schematic diagram of the overall structure of the present invention;

[0026] Figure 2 is a schematic cross-sectional view of the electric heating plate of the present invention;

[0027] Figure 3 is a schematic diagram of the structure of the bottom plate of the present invention;

[0028] Figure 4 is a schematic diagram of the structure of the fan blades under the culture table of the present invention;

[0029] Figure 5 is a schematic diagram of the structure of the exhaust duct and the first runner of the present invention;

[0030] Figure 6 is a schematic diagram of the structure of the exhaust duct and the movable rod of the present invention;

[0031] Figure 7 is a schematic diagram of the connection structure between the first runner and the second runner of the present invention;

[0032] Figure 8 is a schematic diagram of the connection structure of the air inlet pipe of the present invention;

[0033] Figure 9 is a schematic diagram of the connection structure of the air collecting hood and the air guiding pipe of the present invention.

[0034] In the figure: 1, mutation box; 2, box door; 3, electron accelerator; 4, radiation window; 5, mounting rack; 6, bottom plate; 7, support feet; 8, ventilation assembly; 801, electric heating plate; 802, exhaust duct; 803, exhaust fan; 804, exhaust chimney; 805, intake duct; 806, fog chamber; 807, atomizing nozzle; 808, water inlet pipe; 809, filter plate; 810, PLC controller; 811, temperature and humidity sensor; 9, heat and blockage removal assembly; 901, culture table; 902, culture tank; 903, first rotating rod; 904, first bearing; 905, fan blade; 906, first connecting plate; 907, second connecting plate; 908, first screw rod; 909, second bearing; 910, arc-shaped sealing plate; 911, connector; 912, handle; 913, first gasket; 914, air outlet; 915, exhaust hole; 916, movable rod; 917, movable plate; 918, second gasket; 919, spring; 920, rotating shaft; 921, transmission plate; 922, first runner; 923, second rotating rod; 924, third bearing; 925, second runner; 926, third rotating rod; 927, fourth bearing; 928, transmission belt; 929, brush; 930, air collecting hood; 931, air guiding pipe. Detailed implementation manners

[0035] In order to make the technical means, creative features, achieved purposes and functions of the present invention easy to understand, the present invention will be further described below in conjunction with the detailed implementation manners.

[0036] Such as Figure 1 , Figure 2 , Figure 3 , Figure 5 and Figure 8As shown in the figure, a device and method for accelerating microbial mutation based on electron accelerator ray irradiation include a mutation box 1. An electron accelerator 3 is fixedly connected to the upper position of the mutation box 1. A radiation window 4 is fixedly connected to the lower position of the electron accelerator 3. A ventilation component 8 is movably installed in the mutation box 1. The ventilation component 8 includes an air extraction pipe 802, an air extraction fan 803, an exhaust duct 804, an air inlet pipe 805, and a filter plate 809. The air extraction pipe 802 is fixedly connected to the bottom surface of the mutation box 1. The air extraction port of the air extraction fan 803 is fixedly communicated with the air extraction pipe 802, and the exhaust port of the air extraction fan 803 is fixedly communicated with the exhaust duct 804. The air inlet pipe 805 is fixedly connected to the bottom surface of the mutation box 1. The filter plate 809 is fixedly connected to the lower end of the air inlet pipe 805. A box door 2 is movably connected to the front position of the mutation box 1. A bottom plate 6 is fixedly connected to the lower position of the mutation box 1. An installation frame 5 is fixedly connected to the bottom plate 6. The electron accelerator 3 is fixedly connected to the installation frame 5. The bottom plate 6 is fixedly connected to the mutation box 1 through support feet 7. The ventilation component 8 further includes an electric heating plate 801, a mist chamber 806, an atomizing nozzle 807, and a water inlet pipe 808. The electric heating plate 801 is fixedly connected to the inner wall of the mutation box 1. The mist chamber 806 is fixedly connected to the side position of the air inlet pipe 805. One end of the water inlet pipe 808 penetrates through the mist chamber 806 and is fixedly connected with the atomizing nozzle 807. A solenoid valve is movably connected to the water inlet pipe 808. The ventilation component 8 further includes a PLC controller 810 and a temperature and humidity sensor 811. The PLC controller 810 is fixedly connected to the installation frame 5. The temperature and humidity sensor 811 is fixedly connected to the mutation box 1. Place the culture dish in the culture groove 902 of the culture table 901. The PLC controller 810 starts the electrical accelerator 3, so that the electron accelerator 3 irradiates the ray on several culture dishes below through the radiation window 4. The temperature and humidity sensor 811 detects the temperature and humidity in the mutation box 1. The PLC controller 810 controls the electric heating plate 801 to heat up and increase the heat. The PLC controller 810 controls the air extraction fan 803 to work, so that the air flow in the mutation box 1 is extracted, forming a negative pressure environment to absorb the external air flow through the air inlet pipe 805. The solenoid valve on the water inlet pipe 808 is opened through the PLC controller 810. The external liquid water is atomized by the atomizing nozzle 807 and then enters the mutation box 1 along with the air flow, thereby increasing the temperature and humidity concentration in the mutation box 1, so as to provide a ventilated environment with appropriate temperature and humidity for microorganisms.

[0037] As Figure 1 , Figure 3 , Figure 4 , Figure 5 , Figure 6 , Figure 7 and Figure 9As shown, a heat - affected dredging component 9 is movably installed on the mutation box 1. The heat - affected dredging component 9 includes a culture table 901, a first rotating rod 903, a fan blade 905, a first screw rod 908, an arc - shaped sealing plate 910, an air outlet 914, a movable rod 916, a second sealing gasket 918, a transmission plate 921, a first runner 922, a second runner 925 and a brush 929. The culture table 901 is movably connected inside the mutation box 1. The upper and lower ends of the first rotating rod 903 are respectively fixedly connected to the culture table 901 and the fan blade 905. The first screw rod 908 is movably installed with the arc - shaped sealing plate 910, and the arc - shaped sealing plate 910 is movably installed at the side position of the exhaust cylinder 804. The air outlet 914 is opened at one side position of the exhaust cylinder 804. The movable rod 916 is movably installed inside the exhaust cylinder 804. One end of the movable rod 916 is fixedly connected with a second sealing gasket 918, and the other end is movably installed with the first runner 922 through the transmission plate 921. The second runner 925 is movably installed at the side position of the first runner 922. The brush 929 is fixedly connected above the second runner 925. The heat - affected dredging component 9 further includes a culture groove 902 and a first bearing 904. The culture groove 902 is opened on the culture table 901. The first rotating rod 903 is movably installed with the mutation box 1 through the first bearing 904. The heat - affected dredging component 9 further includes a first connecting plate 906, a second connecting plate 907, a second bearing 909, a connecting head 911, a handle 912, a first sealing gasket 913 and an exhaust hole 915. The first connecting plate 906 and the second connecting plate 907 are fixedly connected to the side position of the exhaust cylinder 804. The first screw rod 908 is movably installed with the first connecting plate 906 and the second connecting plate 907 respectively through the second bearing 909. The connecting head 911 is fixedly connected to the side position of the arc - shaped sealing plate 910, and the connecting head 911 is movably installed with the first screw rod 908 through a screw hole. The first sealing gasket 913 is fixedly connected to the inner side position of the arc - shaped sealing plate 910. The handle 912 is fixedly connected to one end position of the first screw rod 908. The exhaust hole 915 is opened on the exhaust cylinder 804. The arc - shaped sealing plate 910 is located at the side position of the exhaust hole 915. The heat - affected dredging component 9 further includes a movable plate 917, a spring 919 and a rotating shaft 920. The movable plate 917 is fixedly connected to one end position of the movable rod 916, and the second sealing gasket 918 is fixedly connected to the movable plate 917. The spring 919 is movably installed on the movable rod 916 and is located inside the exhaust cylinder 804. The spring 919 is located at the side position of the movable plate 917. The two ends of the transmission plate 921 are respectively movably installed with the movable rod 916 and the first runner 922 through the rotating shaft 920. The heat - affected dredging component 9 further includes a second rotating rod 923, a third bearing 924, a third rotating rod 926, a fourth bearing 927 and a rotating belt 928. The upper ends of the second rotating rod 923 and the third rotating rod 926 are respectively movably installed with the bottom surface of the mutation box 1 through the third bearing 924 and the fourth bearing 927. The lower ends of the second rotating rod 923 and the third rotating rod 926 are respectively fixedly connected to the first runner 922 and the second runner 925.The first runner 922 is movably installed with the second runner 925 through a rotating belt 928. The heat dissipation and dredging component 9 further includes an air collecting hood 930 and an air guiding pipe 931. The air collecting hood 930 is fixedly communicated with the air guiding pipe 931. A through hole is provided at a position corresponding to the first rotating rod 903 on the air collecting hood 930, and the fan blade 905 is movably installed in the air collecting hood 930. The air outlet of the air guiding pipe 931 is located below the filter plate 809. When the exhaust duct 804 exhausts air, it pushes the movable plate 917 and the second gasket 918 to the side and reciprocates. The spring 919 is compressed by the movable plate 917, and the movable rod 916 rotates the first runner 922 through the transmission plate 921. When the second movable plate 917 rotates to the side position of the air outlet 914, the air flow starts to be discharged outwards through the air outlet 914 to reduce the air pressure. The spring 919 resets and expands, thereby pushing the movable plate 917, the movable rod 916 and the second gasket 918 in the reverse direction. The first runner 922 drives the second runner 925 to rotate synchronously through the rotating belt 928, so that the brush 929 on the second runner 925 rotates synchronously. The brush 929 brushes under the filter plate 809 to remove the dust on the filter plate 809, so that the filter plate 809 is easy to keep the air intake smooth and not easy to be blocked. The air outlet 914 intermittently discharges gas, blowing the fan blade 905, so that the first rotating rod 903 and the culture table 901 rotate slowly, making the culture dish heated evenly and the culture dish contact more fully with moisture. And the fan blade 905 rotates in the air collecting hood 930, which is convenient to re-introduce part of the air flow to the lower position of the filter plate 809, so that the air flow containing heat is convenient to be recycled into the mutation box 1 through the filter plate 809, facilitating heat recovery and utilization.,

[0038] A method for accelerating microbial mutation based on electron accelerator ray irradiation includes the following steps:

[0039] S1: A culture dish is placed in the culture groove 902 on the culture table 901. After the box door 2 is closed, the temperature and humidity in the mutation box 1 are observed through the temperature and humidity sensor 811. The PLC controller 810 controls the electric heating plate 801 to start to provide heat for microbial culture. The PLC controller 810 controls the exhaust fan 803 to start, so that the exhaust fan 803 sucks the gas in the mutation box 1, making a negative pressure in the mutation box 1, and sucking the external gas through the air inlet pipe 805 to provide ventilation conditions for microbial culture. The PLC controller 810 controls the electron accelerator 3 to start, and irradiates the ray downward to the culture dish through the radiation window 4 for radiation mutagenesis;

[0040] S2: The air inlet pipe 805 filters the external air flow through the filter plate 809 at the lower end, intercepting dust at an external position. The temperature and humidity sensor 811 detects the moisture in the mutation box 1. When it is necessary to increase the moisture concentration, the PLC controller 810 controls the solenoid valve on the water inlet pipe 808 to open. The water inlet pipe 808 is connected to an external water pipe, and the liquid water turns into mist through the atomizing nozzle 807 and enters the mutation box 1 along with the air flow, thereby changing the moisture concentration in the mutation box 1.

[0041] S3: The exhaust fan 803 discharges the air flow in the mutation box 1 into the exhaust duct 804, causing the second gasket 918 and the movable plate 917 to move laterally under the push of the air flow pressure. The movable rod 916 moves synchronously, driving the first runner 922 to rotate through the transmission plate 921. The first runner 922 drives the second runner 925 to rotate through the rotating belt 928, causing the brush 929 on the second runner 925 to rotate synchronously. The filter plate 809 is brushed by the brush 929 to remove dust, preventing the filter plate 809 from being blocked and causing poor air intake.

[0042] S4: During the lateral movement of the movable plate 917 and the movable rod 916, when the movable plate 917 moves to the side position of the air outlet 914, the air flow is discharged from the air outlet 914 and blows against the fan blade 905, causing the fan blade 905 to rotate intermittently. The culture platform 901 rotates actively in the mutation box 1, making the culture dish receive uniform heat and come into more sufficient contact with the water vapor in the air flow.

[0043] S5: The fan blade 905 rotates actively in the air collecting hood 930, causing part of the air flow to pass through the air guiding pipe 931 and be re-introduced to the lower position of the filter plate 809, enabling the heat-containing air flow to be recycled and utilized, facilitating the rapid heating of the temperature in the mutation box 1.

[0044] It should be noted that the present invention is a device and method for accelerating microbial mutation based on electron accelerator ray irradiation. The culture dish is placed in the culture tank 902 of the culture table 901 and fixedly connected. After the box door 2 is closed, the PLC controller 810 starts the electron accelerator 3, so that the electron accelerator 3 irradiates the ray on several culture dishes below through the radiation window 4, thereby better mutating the microorganisms. The temperature and humidity sensor 811 detects the temperature and humidity in the mutation box 1. When it is necessary to provide temperature, the PLC controller 810 controls the electric heating plate 801 to heat, so that the temperature in the mutation box 1 rises. The PLC controller 810 controls the exhaust fan 803 to work, so that the air flow in the mutation box 1 is extracted. As the air in the mutation box 1 is extracted, a negative pressure environment is formed, and the external air flow is absorbed through the air inlet pipe 805. The external air flow removes dust through the filter plate 809 and enters the mutation box 1. When it is necessary to increase the humidity concentration in the box, the solenoid valve on the water inlet pipe 808 is opened through the PLC controller 810. The external liquid water is atomized by the atomizing nozzle 807 and then enters the mutation box 1 along with the air flow, thereby increasing the temperature and humidity concentration in the mutation box 1, providing a well-ventilated environment with suitable temperature and humidity for the microorganisms, and facilitating better cultivation of the microorganisms. When the exhaust fan 803 exhausts air through the exhaust pipe 804, since the air pressure on the side of the second gasket 918 and the movable plate 917 continuously increases as the air flow is continuously discharged, when the air pressure increases to a certain extent, the movable plate 917 and the second gasket 918 are pushed to the side, causing the movable rod 916 to move to the side. The spring 919 is compressed by the movable plate 917, and the movable rod 916 rotates the first runner 922 through the transmission plate 921. When the second movable plate 917 rotates to the side position of the air outlet 914, the air flow starts to be discharged outward through the air outlet 914. At this time, the air pressure pushing the second gasket 918 and the movable plate 917 starts to decrease. By turning the handle 912, the first screw rod 908 rotates, causing the arc-shaped sealing plate 910 to slide on the exhaust pipe 804, thereby appropriately opening some of the several exhaust holes 915 on the side position of the exhaust pipe 804 to increase the air flow velocity, thereby reducing the air pressure. As the air pressure decreases, the spring 919 resets and expands, thereby pushing the movable plate 917, the movable rod 916 and the second gasket 918 in the reverse direction, resulting in a reciprocating movement. The first runner 922 drives the second runner 925 to rotate synchronously through the rotating belt 928, so that the brush 929 on the second runner 925 rotates synchronously. The brush 929 moves and rotates under the filter plate 809, thereby removing the dust on the filter plate 809, making it easier for the filter plate 809 to maintain smooth air intake and not be easily blocked. Since the air outlet 914 intermittently discharges gas, the air flow blows the fan blade 905. Due to the discontinuity of the air flow, the driving force of the air flow is reduced, so that the force on the fan blade 905 is limited.The fan blade 905 drives the first rotating rod 903 to rotate slowly, causing the culture table 901 to rotate synchronously, and making the culture dish receive uniform heat through rotation, so that the culture dish comes into more sufficient contact with moisture. Moreover, the fan blade 905 rotates within the air collecting hood 930, which facilitates introducing some airflows through the air collecting hood 930 and the air guiding pipe 931 back to the position below the filter plate 809 to blow against the filter plate 809, making the dust on the filter plate 809 easier to remove. And some airflows containing heat are conveniently recycled into the mutation box 1 again through the filter plate 809, thereby increasing the heating speed.

[0045] The above shows and describes the basic principles, main features and advantages of the present invention. It is only a preferred embodiment of the present invention, and its description is relatively specific and detailed. However, it cannot be understood as a limitation of the scope of the present invention. Those skilled in the art should understand that the present invention is not limited by the above embodiments. Without departing from the principles and purposes of the present invention, various changes, modifications, substitutions and deformations can be made to the embodiments, and these changes and improvements all fall within the scope of the present invention claimed. The scope of protection of the present invention is defined by the appended claims and their equivalents.

Claims

1. A device for accelerating microbial mutation based on electron accelerator radiation, comprising a mutation box (1), wherein an electron accelerator (3) is fixedly connected to the upper part of the mutation box (1), and a radiation window (4) is fixedly connected to the lower part of the electron accelerator (3), characterized in that: A ventilation assembly (8) is movably installed in the mutation box (1), and the ventilation assembly (8) includes an exhaust fan (803), an air inlet pipe (805) and a filter plate (809). The exhaust fan (803) is fixedly connected to the lower position of the mutation box (1), the air inlet pipe (805) is fixedly connected to the bottom surface of the mutation box (1), and the filter plate (809) is fixedly connected to the lower end of the air inlet pipe (805). A heat-receiving dredging assembly (9) is movably installed on the mutation box (1), and the heat-receiving dredging assembly (9) includes a culture platform (901), a fan blade (905), a No. 1 screw (908), an arc-shaped sealing plate (910), an exhaust port (914), a movable rod (916), a No. 2 sealing pad (918), a transmission plate ( 921), a first rotating wheel (922), a second rotating wheel (925) and a brush (929), the culture platform (901) is movably connected in the mutation box (1), the first screw rod (908) and the arc-shaped sealing plate (910) are movably installed, the exhaust port (914) is opened at a side position of the exhaust cylinder (804), the movable rod (916) is movably installed in the exhaust cylinder (804), one end of the movable rod (916) is fixedly connected to a second sealing gasket (918), and the other end is movably installed with the first rotating wheel (922) through a transmission plate (921), the second rotating wheel (925) is movably installed at a side position of the first rotating wheel (922), and the brush (929) is fixedly connected to an upper position of the second rotating wheel (925).

2. The device for accelerating microbial mutation based on electron accelerator irradiation according to claim 1, characterized in that: The front position of the mutation box (1) is movably connected to a box door (2), the lower position of the mutation box (1) is fixedly connected to a bottom plate (6), the bottom plate (6) is fixedly connected to a mounting frame (5), the electron accelerator (3) is fixedly connected to the mounting frame (5), and the bottom plate (6) is fixedly connected to the mutation box (1) via supporting legs (7).

3. The device for accelerating microbial mutation based on electron accelerator irradiation according to claim 2, characterized in that: The ventilation assembly (8) further comprises an electric heating plate (801), an exhaust pipe (802), an exhaust cylinder (804), a fog bin (806), an atomizing nozzle (807) and a water inlet pipe (808); the exhaust pipe (802) is fixedly connected to the bottom surface of the mutation box (1); the exhaust port of the exhaust fan (803) is fixedly connected to the exhaust pipe (802); and the exhaust port of the exhaust fan (803) is fixedly connected to the exhaust cylinder (804); the electric heating plate (801) is fixedly connected to the inner wall of the mutation box (1); the fog bin (806) is fixedly connected to the side of the air inlet pipe (805); one end of the water inlet pipe (808) passes through the fog bin (806) and is fixedly connected to the atomizing nozzle (807); and the water inlet pipe (808) is movably connected to a solenoid valve.

4. The device for accelerating microbial mutation based on electron accelerator irradiation according to claim 3, characterized in that: The ventilation assembly (8) further comprises a PLC controller (810) and a temperature and humidity sensor (811); the PLC controller (810) is fixedly connected to the mounting frame (5); and the temperature and humidity sensor (811) is fixedly connected to the mutation box (1).

5. The device for accelerating microbial mutation based on electron accelerator irradiation according to claim 4, characterized in that: The heated dredging component (9) further comprises a culture tank (902), a No. 1 rotating rod (903) and a No. 1 bearing (904); the culture tank (902) is arranged on the culture platform (901); the No. 1 rotating rod (903) is movably mounted on the mutation box (1) via the No. 1 bearing (904); the upper end and the lower end of the No. 1 rotating rod (903) are fixedly connected to the culture platform (901) and the fan blade (905) respectively.

6. The device for accelerating microbial mutation based on electron accelerator irradiation according to claim 5, characterized in that: The heat dredging component (9) further comprises a No. 1 connecting plate (906), a No. 2 connecting plate (907), a No. 2 bearing (909), a connector (911), a handle (912), a No. 1 sealing pad (913) and an exhaust hole (915). The No. 1 connecting plate (906) and the No. 2 connecting plate (907) are fixedly connected to the side position of the exhaust cylinder (804), the arc-shaped sealing plate (910) is movably installed at the side position of the exhaust cylinder (804), and the No. 1 screw (908) is respectively connected to the No. 1 connecting plate (906) and the No. 2 connecting plate (907) through the No. 2 bearing (909). 06) The second connecting plate (907) is movably installed, the connecting head (911) is fixedly connected to the side position of the arc-shaped sealing plate (910), and the connecting head (911) is movably installed with the No. 1 screw (908) through the screw hole, the No. 1 sealing gasket (913) is fixedly connected to the inner side position of the arc-shaped sealing plate (910), the handle (912) is fixedly connected to one end position of the No. 1 screw (908), the exhaust hole (915) is opened on the exhaust tube (804), and the arc-shaped sealing plate (910) is located on the side position of the exhaust hole (915).

7. The device for accelerating microbial mutation based on electron accelerator irradiation according to claim 6, characterized in that: The heat dredging component (9) further comprises a movable plate (917), a spring (919) and a rotating shaft (920); the movable plate (917) is fixedly connected to one end of the movable rod (916), and the second sealing gasket (918) is fixedly connected to the movable plate (917); the spring (919) is movably mounted on the movable rod (916) and located in the exhaust duct (804); the spring (919) is located on the side of the movable plate (917); and the two ends of the transmission plate (921) are movably mounted on the movable rod (916) and the first rotating wheel (922) respectively through the rotating shaft (920).

8. The device for accelerating microbial mutation based on electron accelerator irradiation according to claim 7, characterized in that: The heat dredging component (9) further comprises a No. 2 rotating rod (923), a No. 3 bearing (924), a No. 3 rotating rod (926), a No. 4 bearing (927) and a rotating belt (928); the upper ends of the No. 2 rotating rod (923) and the No. 3 rotating rod (926) are movably mounted on the bottom surface of the mutation box (1) through the No. 3 bearing (924) and the No. 4 bearing (927), respectively; the lower ends of the No. 2 rotating rod (923) and the No. 3 rotating rod (926) are fixedly connected to the No. 1 rotating wheel (922) and the No. 2 rotating wheel (925), respectively; and the No. 1 rotating wheel (922) is movably mounted on the No. 2 rotating wheel (925) through the rotating belt (928).

9. The device for accelerating microbial mutation based on electron accelerator irradiation according to claim 8, characterized in that: The heated unblocking component (9) further comprises an air collecting hood (930) and an air duct (931), wherein the air collecting hood (930) is fixedly connected to the air duct (931), a through hole is provided on the air collecting hood (930) at a position corresponding to the first rotating rod (903), and the fan blade (905) is movably installed in the air collecting hood (930), and the air outlet of the air duct (931) is located below the filter plate (809).

10. The method for using the device for accelerating microbial mutation based on electron accelerator irradiation according to any one of claims 1 to 9, characterized in that: The following steps are involved: S1: A culture dish is placed in the culture tank (902) on the culture platform (901). After the box door (2) is closed, the temperature and humidity in the mutation box (1) are observed through the temperature and humidity sensor (811). The PLC controller (810) controls the electric heating plate (801) to start up to increase the heat for microbial culture. The PLC controller (810) controls the exhaust fan (803) to start up so that the exhaust fan (803) sucks the gas in the mutation box (1) to produce a negative pressure in the mutation box (1). The external gas is sucked through the air inlet pipe (805) to provide ventilation conditions for the cultivation of microorganisms. The PLC controller (810) controls the electron accelerator (3) to start up and guides the radiation to the culture dish below through the radiation window (4) to carry out radiation mutagenesis. S2: The air inlet pipe (805) filters the external airflow through the filter plate (809) at the lower end, and filters and intercepts dust at an external position. The temperature and humidity sensor (811) detects the moisture in the mutation box (1). When the moisture concentration needs to be increased, the PLC controller (810) controls the solenoid valve on the water inlet pipe (808) to open, and the water inlet pipe (808) is connected to the external water pipe. The liquid water is converted into mist through the atomizing nozzle (807) and enters the mutation box (1) along with the airflow, thereby changing the moisture concentration in the mutation box (1); S3: The exhaust fan (803) discharges the airflow in the mutation box (1) into the exhaust tube (804), so that the No. 2 sealing pad (918) and the movable plate (917) are pushed to move sideways by the airflow pressure, and the movable rod (916) moves synchronously, driving the No. 1 rotating wheel (922) to rotate through the transmission plate (921), and the No. 1 rotating wheel (922) drives the No. 2 rotating wheel (925) to rotate through the rotating belt (928), so that the brush (929) on the No. 2 rotating wheel (925) rotates synchronously, and the brush (929) brushes the filter plate (809), thereby removing dust and preventing the filter plate (809) from being blocked, causing poor air intake; S4: When the movable plate (917) and the movable rod (916) move to the side, when the movable plate (917) moves to the side position of the exhaust port (914), the airflow is discharged from the exhaust port (914) and blows toward the fan blade (905), so that the fan blade (905) rotates intermittently, and the culture platform (901) rotates in the mutation box (1), so that the culture dish is heated evenly and comes into contact with the water vapor in the airflow more fully; S5: The fan blades (905) move and rotate in the wind collecting cover (930), so that part of the air flow passes through the air duct (931) and is reintroduced to the position below the filter plate (809), so that the air flow containing heat can be recovered and utilized, thereby facilitating rapid heating of the temperature of the mutation box (1).

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