Fluorescence detection system and detection method

Abstract

The invention provides a fluorescence detection system and method, and the system comprises a light wheel assembly which is provided with an excitation channel and a receiving channel; the light source assembly and the receiving assembly are arranged on one side of the light wheel assembly, the light source assembly corresponds to the excitation channel, and the receiving assembly corresponds to the receiving channel; and the optical mechanism is arranged on the other side of the smooth wheel assembly. According to the technical scheme, the excitation channel and the receiving channel are integrated in the same smooth wheel assembly, synchronous switching of the excitation channel and the receiving channel is achieved through the single smooth wheel assembly, the problem of synchronous rotation errors in a traditional double-smooth-wheel structure is avoided, meanwhile, the size of the smooth wheel is reduced, the system structure is simplified, and the positioning precision is improved; the positioning device has the advantages of being simple in structure, high in positioning accuracy and convenient to install and maintain.

Description

Technical Field

[0001] This application relates to the field of biomedical detection technologies, and particularly to a fluorescence detection system and a detection method. Background Art

[0002] At present, multi-color fluorescence PCR instruments on the market are divided into two types: single-channel detectors and multi-channel detectors. The optical detection systems of fluorescence PCR instruments are both composed of two separate parts: an excitation system and a receiving system. The implementation methods for multi-color fluorescence can be roughly divided into two types: dichroic mirror splitting type and optical wheel rotation type.

[0003] For the optical wheel rotation type structure, an independent mode of the excitation optical wheel and the emission optical wheel is adopted, that is, two sets of optical wheel modules are required. Its excitation system structure is: uniformly distribute the seven-color excitation filter common to single-channel and multi-channel on the concentric circles of the excitation optical wheel, correspond the white and ultraviolet dual-color light sources required for the single-channel to one color filter on the excitation optical wheel. During detection, drive the excitation optical wheel to rotate through the excitation motor, so that the single-channel dual-color light sources respectively correspond to the seven-color excitation filters. Its receiving system structure is: uniformly distribute the seven-color detection filters common to single-channel and multi-channel on the concentric circles of the receiving optical wheel, and at the same time correspond the light collecting plate of the single-channel to the position of one color filter. During detection, drive the receiving optical wheel to rotate through the receiving motor, so that the single-channel light collecting plate respectively corresponds to the seven-color detection filters.

[0004] When this fluorescence PCR instrument with an optical wheel rotation type structure is detecting, the excitation optical wheel motor and the receiving optical wheel motor must be controlled separately to achieve real-time synchronous rotation, and the synchronous rotation angle error is relatively large, which is difficult to make up for through motor control. Moreover, since this method requires a dual-motor control mode, the required installation space is relatively large, the part structure is complex, the production cost is relatively high, and there are also problems such as poor positioning accuracy and inconvenient installation and maintenance. Summary of the Invention

[0005] To solve or at least partially solve the above technical problems, a first aspect of this application provides a fluorescence detection system, including:

[0006] An optical wheel assembly, which forms an excitation channel and a receiving channel;

[0007] A light source assembly and a receiving assembly, arranged on one side of the optical wheel assembly, the light source assembly corresponds to the excitation channel, and the receiving assembly corresponds to the receiving channel;

[0008] An optical mechanism is provided on the other side of the optical wheel assembly. The optical mechanism is configured to direct the laser light emitted by the light source assembly and passing through the excitation channel to irradiate a PCR reagent tube to be detected located on the other side of the optical wheel assembly, so as to excite fluorescence, and to direct the fluorescence to pass through the receiving channel and irradiate on the receiving assembly on the same side as the light source assembly.

[0009] Further technical solutions may also be that the excitation channel includes at least a plurality of channels, the receiving channel includes at least a plurality of channels, and the number of the excitation channels is equal to and corresponds one-to-one with the number of the receiving channels;

[0010] The optical wheel assembly can be driven to move so that the light source assembly switches to an adjacent another excitation channel and the receiving assembly switches to an adjacent another receiving channel.

[0011] Further technical solutions may also be that the light source assembly corresponds to at least two of the excitation channels at the same time;

[0012] The receiving assembly corresponds to at least two of the receiving channels at the same time.

[0013] Further technical solutions may also be that the optical wheel assembly can be driven to rotate;

[0014] The plurality of excitation channels are circumferentially and uniformly distributed around the rotation axis of the optical wheel assembly;

[0015] The plurality of receiving channels are circumferentially and uniformly distributed around the rotation axis of the optical wheel assembly.

[0016] Further technical solutions may also be that the fluorescence detection system further includes:

[0017] A first side plate and a second side plate which are oppositely arranged;

[0018] The optical wheel assembly is rotatably arranged between the first side plate and the second side plate;

[0019] The light source assembly and the receiving assembly are arranged on the first side plate, and a light source through hole connected to the excitation channel and a receiving through hole connected to the receiving channel are arranged on the first side plate;

[0020] The optical mechanism is arranged on the second side plate, and an excitation light guiding hole and a receiving light guiding hole are arranged on the second side plate. The number of the excitation light guiding holes is the same as and corresponds one-to-one with the number of the light source through holes, and the number of the receiving light guiding holes is the same as and corresponds one-to-one with the number of the receiving through holes.

[0021] Further technical solutions may also be that the optical mechanism includes:

[0022] The first optical component, one end of the first optical component is connected to the excitation light guiding hole, and the other end is connected to the PCR reagent tube;

[0023] The second optical component, one end of the second optical component is connected to the receiving light guiding hole, and the other end is connected to the PCR reagent tube.

[0024] A further technical solution may also be that both the first optical component and the second optical component include:

[0025] An optical fiber, one end of the optical fiber is connected to the excitation light guiding hole or the receiving light guiding hole, and the other end is connected to the PCR reagent tube, for guiding the laser or the fluorescence;

[0026] An optical fiber pressing plate, sleeved on the optical fiber, for mounting the optical fiber on the excitation light guiding hole or the receiving light guiding hole;

[0027] A rubber ring, sleeved on the optical fiber, for being abutted against the second side plate by the optical fiber pressing plate.

[0028] A further technical solution may also be that the end face inclination angle of the end of the optical fiber connected to the PCR reagent tube is the same as the side inclination angle of the PCR reagent tube.

[0029] The second aspect of the present application also provides a detection method for a fluorescence detection system, including:

[0030] An excitation step: The light source assembly emits laser light from one side of the optical wheel assembly and passes through the excitation channel of the optical wheel assembly to the other side of the optical wheel assembly, and the laser light irradiates the PCR reagent tube to be detected on the other side of the optical wheel assembly under the guidance of the optical mechanism;

[0031] A receiving step: After the PCR reagent tube is irradiated by the laser light, fluorescence is excited, and the fluorescence passes through the receiving channel of the optical wheel assembly under the guidance of the optical mechanism and irradiates on the receiving assembly on the same side as the light source assembly.

[0032] A further technical solution may also be that after the receiving step, it further includes:

[0033] A switching step: Driving the optical wheel assembly to rotate so that the light source assembly switches to another adjacent excitation channel and the receiving assembly switches to another adjacent receiving channel;

[0034] After completing the switching step, repeat the excitation step and the receiving step.

[0035] A further technical solution may also be that the number of the excitation channels is n, where n is an integer divisible by 360 and greater than 7;

[0036] In the switching step, the rotation angle of the optical wheel assembly is m, where m = n / 360°.

[0037] The technical solution of this application integrates the excitation channel and the receiving channel in the same optical wheel assembly, and realizes the synchronous switching of the excitation channel and the receiving channel through a single optical wheel assembly, avoiding the synchronous rotation error problem in the traditional double optical wheel structure. At the same time, it reduces the volume of the optical wheel, simplifies the system structure, improves the positioning accuracy, facilitates installation and maintenance, and has the advantages of simple structure, high positioning accuracy, and convenient installation and maintenance. At the same time, since the excitation channel and the receiving channel are integrated in the same optical wheel assembly, it can ensure that when the optical wheel assembly moves, the excitation channel and the receiving channel provided on the optical wheel assembly can move synchronously, solving the synchronous error problem in the traditional dual-motor control mode and improving the accuracy and stability of detection. Brief Description of the Drawings

[0038] In order to more clearly illustrate the implementation manners of this application, the relevant drawings will be briefly introduced below. It can be understood that the drawings in the following description are only used to illustrate some implementation manners of this application, and those of ordinary skill in the art can also obtain many other technical features and connection relationships not mentioned in this article based on these drawings.

[0039] Figure 1 Structural schematic diagram of a fluorescence detection system provided for an implementation manner of this application;

[0040] Figure 2 Structural schematic diagram of an optical wheel assembly provided for another implementation manner of this application.

[0041] Reference Signs:

[0042] 1. Optical wheel assembly; 11. Excitation channel; 12. Receiving channel; 13. Optical wheel body; 131. Optical wheel plate; 132. Optical wheel cover plate;

[0043] 14. Excitation filter; 15. Receiving filter;

[0044] 2. Light source assembly;

[0045] 3. Receiving assembly;

[0046] 4. Optical mechanism; 41. First optical component; 411. Optical fiber; 412. Optical fiber pressing plate; 413. Rubber ring; 42. Second optical component;

[0047] 5. First side plate;

[0048] 6. Second side plate. Detailed Embodiments

[0049] The technical solutions in the embodiments of the present application will be described in detail below with reference to the accompanying drawings in the embodiments of the present application.

[0050] The inventors of the present application found that in the existing technical solutions, the fluorescence PCR instruments adopting the optical wheel rotation structure all adopt the mode in which the excitation optical wheel and the emission optical wheel are independent. The excitation optical wheel motor and the receiving optical wheel motor need to be controlled separately to achieve real-time synchronous rotation, and the synchronous rotation angle error is relatively large, which is difficult to make up for by motor control. Moreover, since this method requires a dual-motor control mode, the required installation space is relatively large, the part structure is complex, the production cost is relatively high, and there are also problems such as poor positioning accuracy and inconvenient installation and maintenance.

[0051] Embodiment 1

[0052] A first aspect of the first embodiment of the present application provides an optical wheel assembly 1, which is applied to a fluorescence detection system. As Figure 1 and Figure 2 shown, an excitation channel 11 and a receiving channel 12 are formed on the optical wheel assembly 1;

[0053] One side of the optical wheel assembly 1 is used to connect with a light source assembly 2 and a receiving assembly 3, and the other side is used to connect with a PCR reagent tube to be detected. Among them, the light source assembly 2 corresponds to the excitation channel 11, and the receiving assembly 3 corresponds to the receiving channel 12;

[0054] The light emitted by the light source assembly 2 irradiates the PCR reagent tube to be detected on the other side of the optical wheel assembly 1 through the excitation channel 11. The PCR reagent tube excites fluorescence and irradiates the receiving assembly 3 on the same side as the light source assembly 2 through the receiving channel 12.

[0055] Correspondingly, a second aspect of this embodiment also discloses a fluorescence detection system. As Figure 1 shown, it includes:

[0056] An optical wheel assembly 1, on which an excitation channel 11 and a receiving channel 12 are formed;

[0057] A light source assembly 2 and a receiving assembly 3, which are arranged on one side of the optical wheel assembly 1. The light source assembly 2 corresponds to the excitation channel 11, and the receiving assembly 3 corresponds to the receiving channel 12;

[0058] An optical mechanism 4, which is arranged on the other side of the optical wheel assembly 1. The optical mechanism 4 is used to guide the laser emitted by the light source assembly 2 passing through the excitation channel 11 to irradiate the PCR reagent tube to be detected on the other side of the optical wheel assembly 1 to excite fluorescence, and guide the fluorescence to pass through the receiving channel 12 and irradiate the receiving assembly 3 on the same side as the light source assembly 2.

[0059] Accordingly, a third aspect of this embodiment also discloses a detection method for a fluorescence detection system, including:

[0060] Excitation step: The light source assembly 2 emits laser light from one side of the optical wheel assembly 1 and passes through the excitation channel 11 of the optical wheel assembly 1 to the other side of the optical wheel assembly 1, and the laser light irradiates the PCR reagent tube to be detected on the other side of the optical wheel assembly 1 under the guidance of the optical mechanism 4;

[0061] Receiving step: After being irradiated by the laser light, the PCR reagent tube emits fluorescence, and the fluorescence passes through the receiving channel 12 of the optical wheel assembly 1 under the guidance of the optical mechanism 4 and irradiates on the receiving assembly 3 on the same side as the light source assembly 2.

[0062] In the prior art, the excitation channel and the receiving channel are respectively arranged on two different optical wheels, and two motors are used to drive the two optical wheels to rotate respectively to switch the excitation channel and the receiving channel. However, the solution adopted in this embodiment is to jointly arrange the excitation channel 11 and the receiving channel 12 on the same optical wheel assembly 1, the light source assembly 2 and the receiving assembly 3 are arranged on one side of the optical wheel assembly 1, and the PCR reagent tube to be detected is arranged on the other side of the optical wheel assembly 1. In this way, the light emitted by the light source assembly 2 can irradiate the PCR reagent tube to be detected on the other side of the optical wheel assembly 1 through the excitation channel 11, and the PCR reagent tube emits fluorescence and irradiates on the receiving assembly 3 on the same side as the light source assembly 2 through the receiving channel 12.

[0063] It is worth mentioning that in the prior art, the path of light on the optical wheel is in a single direction, that is, the light source assembly emits laser light from one side of the optical wheel and passes through the optical wheel, and the fluorescence excited by the PCR reagent tube is received by the receiving assembly on the other side of the optical wheel. However, in this embodiment, the path of light on the optical wheel assembly 1 is two-way, that is, the light source assembly 2 emits laser light from one side of the optical wheel and passes through the optical wheel to irradiate the PCR reagent tube on the other side of the optical wheel assembly 1 and excites fluorescence, and the fluorescence passes through the optical wheel assembly 1 in the opposite direction of the laser light and irradiates on the receiving assembly 3 on the same side as the light source assembly 2. In this way, the number of optical wheel assemblies 1 can be reduced, thereby effectively reducing the volume of the fluorescence detection system.

[0064] In this embodiment, the optical mechanism 4 is used to guide the laser emitted by the light source assembly 2 and the fluorescence excited by the PCR reagent tube. Specifically, the optical mechanism 4 may include a first optical component 41 and a second optical component 42 with similar structures. Among them, the first optical component 41 is used to irradiate the laser emitted from the excitation channel 11 onto the PCR reagent tube, so that the PCR reagent tube excites fluorescence. The second optical component 42 is used to guide the fluorescence excited by the PCR reagent tube to the receiving channel 12, so that the fluorescence can pass through the receiving channel 12 and irradiate on the receiving component 3. Since the structures of the first optical component 41 and the second optical component 42 are similar, the first optical component 41 will be taken as an example for illustration. Specifically, in some embodiments, the first optical component 41 may include a focusing lens for focusing light and a refracting lens for refracting light to change the propagation angle of the light.

[0065] Embodiment 2

[0066] The inventor found that in existing fluorescence detection systems, it is usually necessary to set up multiple excitation channels 11 and receiving channels 12 in one-to-one correspondence. Multiple filters of different colors are arranged inside the excitation channels 11 and the receiving channels 12 to filter the laser emitted by the light source assembly 2 and output laser of a set color. Subsequently, the optical wheel is rotated by a motor to enable the light source assembly 2 to switch to different excitation channels 11 and the receiving component 3 to switch to different receiving channels 12, realizing multi-channel detection.

[0067] This embodiment is a further improvement based on the first embodiment to meet the requirements of multi-channel detection. Specifically, as Figure 1 shown, the excitation channels 11 include at least multiple ones, the receiving channels 12 include at least multiple ones, and the number of the excitation channels 11 is equal to and in one-to-one correspondence with the number of the receiving channels 12;

[0068] The optical wheel assembly 1 can be driven to move, so that the light source assembly 2 switches to another adjacent excitation channel 11 and the receiving component 3 switches to another adjacent receiving channel 12.

[0069] By driving the movement of the optical wheel assembly 1, the light source assembly 2 and the receiving assembly 3 can be respectively switched to adjacent excitation channels 11 and receiving channels 12 to achieve multi-channel detection. Specifically, the optical wheel assembly 1 can be rotated or linearly moved by a motor or other driving device, so that the light source assembly 2 and the receiving assembly 3 are switched between different channels. Moreover, in this embodiment, since the excitation channel 11 and the receiving channel 12 are integrated on the same optical wheel assembly 1, the synchronous rotation error problem in the traditional double-optical-wheel structure is avoided. At the same time, the volume of the optical wheel is reduced, the system structure is simplified, the positioning accuracy is improved, the installation and maintenance are facilitated, and it has the advantages of simple structure, high positioning accuracy, and convenient installation and maintenance.

[0070] There can be various implementation methods for the driving method of the optical wheel assembly 1. For example, a stepper motor can be used to drive the rotation of the optical wheel assembly 1, or a linear driver can be used to achieve the translational movement of the optical wheel assembly 1. Further, the movement of the optical wheel assembly 1 can be accurately controlled by a control system to ensure the accurate positioning of the light source assembly 2 and the receiving assembly 3 when switching channels.

[0071] Compared with the prior art, in this embodiment, the excitation channel 11 and the receiving channel 12 are integrated on the same optical wheel assembly 1, and the synchronous switching of the excitation channel 11 and the receiving channel 12 is achieved through a single optical wheel assembly 1. The synchronous rotation error problem in the traditional double-optical-wheel structure is avoided. At the same time, the volume of the optical wheel is reduced, the system structure is simplified, the positioning accuracy is improved, the installation and maintenance are facilitated, and it has the advantages of simple structure, high positioning accuracy, and convenient installation and maintenance. At the same time, since the excitation channel 11 and the receiving channel 12 are integrated on the same optical wheel assembly 1, it can be ensured that when the optical wheel assembly 1 moves, the excitation channel 11 and the receiving channel 12 provided on the optical wheel assembly 1 can move synchronously, solving the synchronous error problem in the traditional dual-motor control mode and improving the accuracy and stability of detection.

[0072] It is worth mentioning that in some embodiments, the light source assembly 2 corresponds to at least two of the excitation channels 11 at the same time;

[0073] The receiving assembly 3 corresponds to at least two of the receiving channels 12 at the same time.

[0074] Specifically, the light source assembly 2 can be a dual-channel dual-color light source assembly. The light source assembly 2 has two laser-emitting ports respectively corresponding to two excitation channels 11, and can respectively emit lasers to the two excitation channels 11 for fluorescence detection. Correspondingly, the receiving assembly 3 has two fluorescence-receiving ports respectively corresponding to two receiving channels 12. In this way, each time the optical wheel assembly 1 moves, dual-color fluorescence detection can be completed.

[0075] In some embodiments, the optical wheel assembly 1 can be driven to rotate;

[0076] A plurality of the excitation channels 11 are circumferentially distributed about the rotation axis of the optical wheel assembly 1;

[0077] A plurality of the receiving channels 12 are circumferentially distributed about the rotation axis of the optical wheel assembly 1.

[0078] Correspondingly, after the receiving step, the following steps are further included:

[0079] Switching step: Driving the optical wheel assembly 1 to rotate so that the light source assembly 2 is switched to another adjacent excitation channel 11 and the receiving assembly 3 is switched to another adjacent receiving channel 12;

[0080] After completing the switching step, the excitation step and the receiving step are repeated.

[0081] In this embodiment, the optical wheel assembly 1 can be driven to rotate so that the excitation channel 11 and the receiving channel 12 can be switched during the detection process. The plurality of excitation channels 11 and receiving channels 12 are distributed on the circumference of the optical wheel assembly 1. Such a design can ensure that each excitation channel 11 and receiving channel 12 can correspond to the light source assembly 2 and the receiving assembly 3 in sequence when the optical wheel assembly 1 rotates, so as to realize the multi-channel fluorescence detection function.

[0082] In some more preferred embodiments, a plurality of the excitation channels 11 are evenly circumferentially distributed about the rotation axis of the optical wheel assembly 1;

[0083] A plurality of the receiving channels 12 are evenly circumferentially distributed about the rotation axis of the optical wheel assembly 1.

[0084] In this embodiment, the excitation channel 11 and the receiving channel 12 are arranged in a form of being evenly circumferentially distributed about the rotation axis of the optical wheel assembly 1, so that when the optical wheel assembly 1 rotates by a certain angle, the light source assembly 2 and the receiving assembly 3 can correspond to the plurality of excitation channels 11 and receiving channels 12 in sequence. This not only improves the detection accuracy, but also simplifies the structural design and reduces the complexity of synchronous control.

[0085] In addition, it is worth mentioning that in this embodiment, both the light source assembly 2 and the receiving assembly 3 are arranged on the same side of the optical wheel assembly 1. Therefore, in order to successfully install the light source assembly 2 and the receiving assembly 3 on the same side of the optical wheel assembly 1 and ensure that both can work properly without interfering with each other, it is necessary to limit the positions of the plurality of excitation channels 11 and the receiving channels 12. Specifically, the circles where the plurality of excitation channels 11 are located are concentric with the circles where the plurality of receiving channels 12 are located but have different diameters. Through the above settings, the excitation channels 11 and the receiving channels 12 can be staggered from each other in the radial direction of the optical wheel assembly 1, thereby creating a necessary installation space for the light source assembly 2 and the receiving assembly 3 to ensure that both can work properly without interfering with each other, and such settings can also effectively reduce the volume of the system.

[0086] In some other preferred embodiments, any one of the excitation channels 11 is located on the line connecting the midpoint of the line between two adjacent receiving channels 12 and the rotation axis of the optical wheel assembly 1.

[0087] Through the above settings, the plurality of excitation channels 11 and the receiving channels 12 can be staggered from each other in the circumferential direction of the optical wheel assembly 1. On the one hand, it can further create a necessary installation space for the light source assembly 2 and the receiving assembly 3; on the other hand, it can also effectively reduce the diameter of the optical wheel assembly 1, thereby reducing the volume of the system.

[0088] As Figure 1 and Figure 2 shown, the optical wheel assembly 1 may include:

[0089] An optical wheel body 13;

[0090] An excitation filter 14, which is arranged on the optical wheel body 13 and is located within the excitation channel 11;

[0091] A receiving filter 15, which is arranged on the optical wheel body 13 and is located within the receiving channel 12.

[0092] The optical wheel body 13 includes:

[0093] An optical wheel plate 131, on which filter mounting grooves are provided for mounting the excitation filter 14 and the receiving filter 15;

[0094] An optical wheel cover plate 132, which is connected to the optical wheel plate 131 to cooperate with the optical wheel plate 131 to fix the excitation filter 14 and the receiving filter 15.

[0095] As can be seen from the above, the excitation channels 11 and the receiving channels 12 are evenly distributed around the circumference of the optical wheel assembly 1. And when the optical wheel assembly 1 rotates by a certain angle, the light source assembly 2 and the receiving assembly 3 can successively correspond to a plurality of excitation channels 11 and receiving channels 12. In the actual application process, usually a driving motor is used to drive the rotation of the optical wheel assembly 1. Therefore, in order to facilitate the control of the driving motor rotation and ensure the rotation accuracy of the driving motor, it is necessary to limit the number of the excitation channels 11 and the receiving channels 12. Specifically, the number of the excitation channels 11 is an integer that can be divisible by 360 and is greater than 7, and the number of the receiving channels 12 is the same as that of the excitation channels 11 and corresponds one by one. Specifically, the number of the excitation channels 11 is n, where n is an integer that can be divisible by 360 and is greater than 7.

[0096] The number of the excitation channels 11 is n, where n can be divisible by 360. In this way, if the optical wheel rotates once, the light source assembly 2 switches to another adjacent excitation channel 11 and the receiving assembly 3 switches to another adjacent receiving channel 12; at this time, the rotation angle of the optical wheel assembly 1 is m, where m = n / 360°.

[0097] It should be additionally noted that in the existing multi-channel fluorescence detection system, usually seven-color fluorescence detection needs to be performed. Therefore, at least 7 excitation channels 11 and receiving channels 12 are provided. Therefore, setting the number n of the excitation channels 11 as an integer that can be divisible by 360 and is greater than 7 can meet the requirements of the fluorescence detection system. In some embodiments, the number n of the excitation channels 11 is 8. Specifically, 8 excitation channels 11 and 8 receiving channels 12 corresponding to the excitation channels 11 one by one are provided on the optical wheel assembly 1. Among them, any 7 excitation channels 11 and the corresponding 7 receiving channels 12 are used to meet the seven-color fluorescence detection, and the remaining one excitation channel 11 and the receiving channel 12 can be used as spare channels to meet additional detection requirements.

[0098] For example, as Figure 2 shown, the optical wheel body 13 has three concentric circles respectively, where the center of the circle is used to connect with the driving motor. 8 excitation channels 11 are distributed on the outer circle of the optical wheel body 13, and 8 receiving channels 12 are distributed on the middle circle. Different-color excitation filter plates 14 are successively installed in the 8 excitation channels 11, and different-color receiving filter plates 15 are successively installed in the 8 receiving channels 12, and the excitation filter plates 14 in the excitation channels 11 can correspond to the receiving filter plates 15 in the receiving channels 12 one by one. When performing fluorescence detection, the optical wheel assembly 1 rotates 45° each time, and the corresponding excitation channels 11 and receiving channels 12 can excite and receive fluorescence once; when the optical wheel assembly 1 rotates one circle, the seven-color fluorescence detection can be completed.

[0099] It should be noted that the number of excitation channels 11 and receiving channels 12 is not limited to 8, and can also be other numbers greater than 8 that meet the above conditions. Among them, any 7 excitation channels 11 and the corresponding 7 receiving channels 12 are used to meet the seven-color fluorescence detection, and the remaining excitation channels 11 and receiving channels 12 can be used as redundancy. On the one hand, it is used to meet other additional fluorescence detection requirements, and on the other hand, it can be used as a substitute when any excitation channel 11 or excitation channel 11 fails to work.

[0100] Embodiment 3

[0101] This embodiment is a further improvement based on the second embodiment. The improvement lies in that, as Figure 1 shown, the fluorescence detection system further includes:

[0102] A first side plate 5 and a second side plate 6 arranged oppositely;

[0103] The optical wheel assembly 1 is rotatably arranged between the first side plate 5 and the second side plate 6;

[0104] The light source assembly 2 and the receiving assembly 3 are arranged on the first side plate 5. The first side plate 5 is provided with a light source through hole connected to the excitation channel 11 and a receiving through hole connected to the receiving channel 12;

[0105] The optical mechanism 4 is arranged on the second side plate 6. The second side plate 6 is provided with an excitation light guiding hole and a receiving light guiding hole. The number of excitation light guiding holes is the same as and corresponds to the light source through holes one by one, and the number of receiving light guiding holes is the same as and corresponds to the receiving through holes one by one.

[0106] The technical solution of the present application enables the optical wheel assembly 1 to rotate between the two side plates by setting the first side plate 5 and the second side plate 6. The light source assembly 2 and the receiving assembly 3 are respectively arranged on the first side plate 5 and are connected to the excitation channel 11 and the receiving channel 12 of the optical wheel assembly 1 through the light source through hole and the receiving through hole. The optical mechanism 4 is arranged on the second side plate 6 and is connected to the excitation channel 11 and the receiving channel 12 of the optical wheel assembly 1 through the excitation light guiding hole and the receiving light guiding hole. The purpose of such a design is to enable the light emitted by the light source assembly 2 to irradiate on the PCR reagent tube to be detected through the excitation channel 11 during the detection process, and the fluorescence excited by the PCR reagent tube to irradiate on the receiving assembly 3 through the receiving channel 12.

[0107] Specifically, the optical wheel assembly 1 can rotate between the first side plate 5 and the second side plate 6. The light source assembly 2 and the receiving assembly 3 are connected to the excitation channel 11 and the receiving channel 12 of the optical wheel assembly 1 through the light source through-hole and the receiving through-hole respectively. The optical mechanism 4 is connected to the excitation channel 11 and the receiving channel 12 of the optical wheel assembly 1 through the excitation light guide hole and the receiving light guide hole respectively, so that the light emitted by the light source assembly 2 irradiates on the PCR reagent tube through the excitation channel 11, and the excited fluorescence irradiates on the receiving assembly 3 through the receiving channel 12.

[0108] Thus, through this design, the traditional dual-motor control mode is avoided, the installation space is reduced, the production cost is lowered, the structure is simplified, the positioning accuracy is improved, and the installation and maintenance are facilitated. At the same time, the rotation of the excitation channel 11 and the receiving channel 12 is synchronously driven by the optical wheel, avoiding the real-time control error between the excitation optical wheel motor and the receiving optical wheel motor, and improving the accuracy and reliability of detection.

[0109] Correspondingly, one end of the first optical component 41 is connected to the excitation light guide hole, and the other end is connected to the PCR reagent tube;

[0110] One end of the second optical component 42 is connected to the receiving light guide hole, and the other end is connected to the PCR reagent tube.

[0111] In some embodiments, the first optical component 41 and the second optical component 42 can use optical fibers to transmit light. Specifically, as Figure 1 shown, both the first optical component 41 and the second optical component 42 include:

[0112] An optical fiber 411, one end of the optical fiber 411 is connected to the excitation light guide hole or the receiving light guide hole, and the other end is connected to the PCR reagent tube, for guiding the laser or the fluorescence;

[0113] An optical fiber pressing plate 412, sleeved on the optical fiber 411, for installing the optical fiber 411 on the excitation light guide hole or the receiving light guide hole;

[0114] A rubber ring 413, sleeved on the optical fiber 411, for being abutted against the second side plate 6 by the optical fiber pressing plate 412.

[0115] In this embodiment, an optical fiber 411 is used as a light guiding element. The laser is guided to the PCR reagent tube through the optical fiber 411, or the fluorescence is guided from the PCR reagent tube to the receiving light guiding hole. The optical fiber pressing plate 412 is used to fix the position of the optical fiber 411 to ensure that the optical fiber 411 can stably transmit the optical signal. The rubber ring 413 provides additional fixing and sealing functions to prevent the optical fiber 411 from loosening or leaking light during installation. Through this design, the transmission of the optical signal can be effectively achieved, and the structure is relatively simple, making installation and maintenance more convenient.

[0116] Furthermore, the other end of the optical fiber 411 is connected to the PCR reagent tube for guiding the laser or fluorescence. The optical fiber pressing plate 412 is sleeved on the optical fiber 411 to fix the optical fiber 411 on the excitation light guiding hole or the receiving light guiding hole. The rubber ring 413 is sleeved on the optical fiber 411 and is used to be abutted against the second side plate 6 by the optical fiber pressing plate 412. The use of the optical fiber 411 makes the transmission of the laser and fluorescence more efficient and stable. The design of the optical fiber pressing plate 412 and the rubber ring 413 ensures the stability and sealing of the optical fiber 411, avoiding the loss and interference of the optical signal. In this embodiment, the use of the optical fiber 411 improves the efficiency and stability of the optical signal transmission. The design of the optical fiber pressing plate 412 and the rubber ring 413 ensures the fixing and sealing of the optical fiber 411, making the entire system more reliable and easy to maintain.

[0117] In some preferred embodiments, the end face inclination angle of the end of the optical fiber 411 connected to the PCR reagent tube is the same as the side face inclination angle of the PCR reagent tube. The purpose of this design is to ensure that the end face of the optical fiber 411 can closely fit the side face of the PCR reagent tube, improve the optical transmission efficiency, reduce light loss, and thus improve the detection accuracy. Through this design, the connection between the optical fiber 411 and the PCR reagent tube can be ensured to be stable and reliable, effectively avoiding light leakage and detection errors caused by the gap between the optical fiber 411 and the reagent tube.

[0118] Specifically, the end face of the optical fiber 411 can be processed into the same inclination angle as the side face of the PCR reagent tube through a precise angle cutting process. In this way, during installation, the end face of the optical fiber 411 can completely fit the side face of the reagent tube, ensuring the efficient transmission of the optical signal, reducing the return loss, and maximizing the signal-to-noise ratio, which is beneficial to improving the detection lower limit of the reagent. At the same time, the inclination angle design of the end face of the optical fiber 411 can be adjusted according to the shapes and sizes of different PCR reagent tubes to meet different application requirements.

[0119] For those skilled in the art, it is obvious that the present application is not limited to the details of the above-described exemplary embodiments, and that the present application can be implemented in other specific forms without departing from the spirit or essential characteristics of the present application. Therefore, from any point of view, the embodiments should be regarded as exemplary and non-restrictive. The scope of the present application is defined by the appended claims rather than the above description. Therefore, all changes falling within the meaning and scope of the equivalent elements of the claims are intended to be embraced within the present application. Any reference signs in the claims should not be construed as limiting the claims involved.

Claims

1. A fluorescence detection system, characterized in that: include: A light wheel assembly, wherein the light wheel assembly is formed with an excitation channel and a receiving channel; A light source assembly and a receiving assembly are arranged on one side of the optical wheel assembly, the light source assembly corresponds to the excitation channel, and the receiving assembly corresponds to the receiving channel; An optical mechanism is arranged on the other side of the light wheel assembly, and is used to guide the laser emitted by the light source assembly through the excitation channel to irradiate the PCR reagent tube to be detected located on the other side of the light wheel assembly to excite fluorescence, and guide the fluorescence to pass through the receiving channel and irradiate the receiving assembly on the same side as the light source assembly.

2. The fluorescence detection system according to claim 1, characterized in that: The excitation channels include at least a plurality of channels, the receiving channels include at least a plurality of channels, and the number of the excitation channels is equal to the number of the receiving channels and they correspond one to one; The optical wheel assembly can be driven to move so that the light source assembly switches to another adjacent excitation channel and the receiving assembly switches to another adjacent receiving channel.

3. The fluorescence detection system according to claim 2, characterized in that: The light source assembly corresponds to at least two of the excitation channels simultaneously; The receiving component corresponds to at least two of the receiving channels at the same time.

4. The fluorescence detection system according to claim 2, characterized in that: The optical wheel assembly can be driven to rotate; The plurality of excitation channels are evenly distributed around the circumference of the rotating axis of the optical wheel assembly; The plurality of receiving channels are evenly distributed around the circumference of the rotating axis of the optical wheel assembly.

5. The fluorescence detection system according to claim 3, characterized in that: Also includes: A first side plate and a second side plate arranged opposite to each other; The optical wheel assembly is rotatably disposed between the first side plate and the second side plate; The light source assembly and the receiving assembly are arranged on the first side plate, and the first side plate is provided with a light source through hole connected to the excitation channel and a receiving through hole connected to the receiving channel; The optical mechanism is arranged on the second side plate, and the second side plate is provided with excitation light guide holes and receiving light guide holes, the number of the excitation light guide holes is the same as that of the light source through holes and they correspond one to one, and the number of the receiving light guide holes is the same as that of the receiving through holes and they correspond one to one.

6. The fluorescence detection system according to claim 5, characterized in that: The optical mechanism comprises: A first optical component, one end of which is connected to the excitation light guide hole, and the other end of which is connected to the PCR reagent tube; A second optical component, one end of the second optical component is connected to the receiving light guide hole, and the other end is connected to the PCR reagent tube.

7. The fluorescence detection system according to claim 6, characterized in that: The first optical component and the second optical component both include: An optical fiber, one end of which is connected to the excitation light guide hole or the receiving light guide hole, and the other end of which is connected to the PCR reagent tube, for guiding the laser or the fluorescence; An optical fiber pressing plate, sleeved on the optical fiber, and used for installing the optical fiber on the excitation light guide hole or the receiving light guide hole; The rubber ring is sleeved on the optical fiber and is used to be abutted against the second side plate by the optical fiber pressing plate.

8. The fluorescence detection system according to claim 7, characterized in that: The inclination angle of the end face of the end of the optical fiber connected to the PCR reagent tube is the same as the inclination angle of the side face of the PCR reagent tube.

9. A detection method for a fluorescence detection system, characterized in that: include: Excitation step: the light source assembly emits laser light from one side of the light wheel assembly and passes through the excitation channel of the light wheel assembly to the other side of the light wheel assembly, and the laser light is irradiated on the PCR reagent tube to be detected on the other side of the light wheel assembly under the guidance of the optical mechanism; Receiving step: the PCR reagent tube is stimulated to emit fluorescence after being irradiated by the laser, and the fluorescence passes through the receiving channel of the optical wheel assembly under the guidance of the optical mechanism and irradiates the receiving assembly on the same side as the light source assembly.

10. The detection method according to claim 9, characterized in that: After the receiving step, the method further includes: Switching step: driving the optical wheel assembly to rotate so that the light source assembly switches to another adjacent excitation channel and the receiving assembly switches to another adjacent receiving channel; After completing the switching step, repeat the exciting step and the receiving step.

11. The detection method according to claim 10, characterized in that: The number of the excitation channels is n, where n is an integer divisible by 360 and greater than 7; In the switching step, the rotation angle of the optical wheel assembly is m, where m=n / 360°.

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