Plant oil essential oil composition and use

Abstract

The present application belongs to the technical field of daily chemicals, and discloses a plant oil essential oil composition and application. The plant oil essential oil composition comprises the following components in parts by weight: 50-80 parts of deep-sea two-joint shepherd's purse seed oil, 5-20 parts of rust red rose seed oil, 8-15 parts of evening primrose oil, 2-15 parts of Schizandrae root bark extract, and 1.2-5 parts of jasmine extract. The plant oil essential oil composition added into massage oil can penetrate into the skin bottom layer through the synergistic effect of various active ingredients, inhibit the production and release of inflammatory factors, reduce the inflammatory reaction of the skin, relieve symptoms such as redness, pain and itching caused by inflammation, balance the secretion of skin oil, promote the blood circulation of the skin, eliminate fatigue, increase the elasticity and luster of the skin, reduce the formation of color spots and dullness, and make the skin brighter and more uniform.

Description

Technical Field

[0001] This invention relates to the field of daily chemical products technology, specifically to a plant oil essential oil composition and its application. Background Technology

[0002] Essential oils are volatile aromatic substances extracted from the flowers, leaves, stems, roots, or fruits of plants through steam distillation, expression, cold maceration, or solvent extraction. Essential oils are composed of many different molecules, the structure and properties of which determine their aroma, efficacy, and permeability. For example, rose essential oil can be composed of more than 250 different molecules, giving it its unique aroma and effects. Essential oils are lipophilic, readily soluble in oils, and their short molecular chains allow them to quickly penetrate the skin and enter the bloodstream through capillaries in subcutaneous fat, exerting various effects such as antibacterial, anti-inflammatory, and antioxidant properties, thereby maintaining health. Furthermore, the small molecules of essential oils can be absorbed into the body through the nasal mucosa, directly affecting the limbic system of the brain, thus regulating mood and physiological functions. For example, in aromatherapy, essential oils are widely used to enhance physiological and psychological functions. Using essential oils through inhalation, massage, or bathing can help relieve stress, improve sleep, enhance concentration, and alleviate pain.

[0003] With the improvement of living standards, people's demand for essential oil products is constantly increasing, especially when facing health issues such as skin care, mood management, respiratory system maintenance, and daily body care. There is a strong demand for highly effective, safe, and natural essential oil products. However, traditional essential oil products often rely on chemically synthesized compounds, which not only weakens the natural advantages of essential oils and fails to unleash their true efficacy, but also poses potential threats to human health. Long-term use of these products can lead to adverse effects such as skin allergies, barrier damage, and liver and kidney dysfunction. Furthermore, currently available essential oils often have limited efficacy, requiring people to purchase multiple essential oils to meet various needs. Therefore, there is an urgent need to develop safer, more effective essential oils that can achieve multiple benefits, meeting the market's demand for high-quality health products while promoting the sustainable development of the essential oil industry. Summary of the Invention

[0004] The purpose of this invention is to overcome the shortcomings of the prior art and provide a plant oil essential oil composition and its application.

[0005] To achieve the above objectives, the technical solution adopted by the present invention is as follows:

[0006] In a first aspect, the present invention provides a plant oil essential oil composition comprising the following components in parts by weight: 50-80 parts of deep-sea two-section shepherd's purse seed oil, 5-20 parts of rust-red rose seed oil, 8-15 parts of evening primrose oil, 2-15 parts of Acanthopanax senticosus root bark extract and 1.2-5 parts of jasmine flower extract.

[0007] The plant oil essential oil composition of this invention achieves significant anti-inflammatory, soothing, anti-wrinkle, and antioxidant effects through the synergistic effect of multiple active ingredients, including deep-sea shepherd's purse seed oil, rosehip seed oil, evening primrose oil, eleutherococcus root bark extract, and jasmine flower extract. Furthermore, all its active ingredients are natural plant oils or plant oil extracts, avoiding the use of chemically synthesized substances and reducing the risk of skin and body irritation and side effects. It is suitable for various needs such as mood management, pain relief, strengthening tendons and ligaments, promoting blood circulation, and skin care, and has good safety and gentleness. Among them, the deep-sea shepherd's purse seed oil is rich in long-chain fatty acid C22, which, in synergy with other components, improves and enhances the skin's antioxidant capacity and natural stability, preventing damage from external harmful substances. It also gives the composition excellent skin affinity, allowing it to easily penetrate deep into the skin to provide essential nutrients and moisture. The rust-red rose seed oil, in synergy with other components, effectively exerts deep moisturizing, antioxidant, and anti-aging effects, improving skin health. Evening primrose oil, in addition to palmitic acid, oleic acid, and stearic acid, contains approximately 70% linoleic acid and about 9% gamma-linolenic acid. Gamma-linolenic acid is one of the polyunsaturated fatty acids needed by the human body. It can be converted into arachidonic acid in the body, providing a precursor for the formation of prostaglandins and biomembranes; the root bark extract of Acanthopanax senticosus is rich in polysaccharides, isoflavones, chlorogenic acid, sesamin, stearic acid, β-sitosterol and other active substances. Evening primrose oil can be combined with Acanthopanax senticosus root bark extract to further synergize with other components to inhibit the production and release of inflammatory mediators, regulate inflammatory responses, scavenge free radicals, protect the skin from oxidative damage, and effectively exert the effects of enhancing immunity, anti-inflammation, soothing and anti-oxidation; Jasmine flower extract works with other components to resist free radical damage, slow down the aging process, reduce skin inflammation, promote skin repair, and effectively exert anti-inflammatory, soothing and anti-oxidation effects.

[0008] Therefore, through the synergistic effect of multiple components, the plant-based essential oil composition of this invention can penetrate deep into the skin to inhibit the production and release of inflammatory factors, reduce skin inflammation, and soothe symptoms such as redness, swelling, pain, and itching caused by inflammation, promoting skin repair and regeneration. Simultaneously, they can balance sebum secretion, alleviate skin sensitivity and discomfort, promote blood circulation, and stimulate collagen synthesis and elastin production, reducing the formation of fine lines and wrinkles, and increasing skin elasticity and radiance. Furthermore, they can scavenge free radicals, protect the skin from oxidative damage, delay the skin aging process, reduce the formation of age spots and dullness, and make the skin brighter and more even-toned. In summary, this invention, through the rational compounding of multiple active ingredients, not only successfully constructs a powerful, multi-functional essential oil system but also effectively enhances the skin's barrier function and overall health.

[0009] As a preferred embodiment of the plant oil essential oil composition of the present invention, the plant oil essential oil composition comprises the following components in parts by weight: 56-78.8 parts of deep-sea two-jointed shepherd's purse seed oil, 9-17 parts of rust-red rose seed oil, 8-15 parts of evening primrose oil, 3-8 parts of Acanthopanax senticosus root bark extract and 1.2-4 parts of jasmine flower extract.

[0010] Preferably, the plant oil essential oil composition comprises the following components in parts by weight: 67.14 parts of deep-sea two-sectioned shepherd's purse seed oil, 13.16 parts of rust-red rose seed oil, 11.28 parts of evening primrose oil, 5.64 parts of Acanthopanax senticosus root bark extract and 2.78 parts of jasmine flower extract.

[0011] As a preferred embodiment of the plant oil essential oil composition of the present invention, the mass ratio of the evening primrose oil to the extract of Acanthopanax senticosus root bark is 1:(0.15-1.5).

[0012] Preferably, the mass ratio of the evening primrose oil to the eleutherococcus root bark extract is 1:(0.375-0.75).

[0013] More preferably, the mass ratio of the evening primrose oil to the Acanthopanax senticosus root bark extract is any one or a combination of 1:0.375, 1:0.4, 1:0.425, 1:0.45, 1:0.475, 1:0.5, 1:0.525, 1:0.55, 1:0.575, 1:0.6, 1:0.65, 1:0.7, and 1:0.75.

[0014] Secondly, the present invention provides a method for preparing the plant oil essential oil composition, comprising the following steps: mixing the deep-sea shepherd's purse seed oil, rosehip seed oil, evening primrose oil, eleutherococcus root bark extract and jasmine flower extract evenly to obtain the final product.

[0015] Thirdly, the present invention provides a massage oil comprising the aforementioned plant oil and essential oil composition.

[0016] The massage oil of the present invention provides a safe and effective massage oil that achieves anti-inflammatory, soothing, anti-wrinkle, and antioxidant effects by rationally combining the plant oil and essential oil composition of the present invention with other additives, and has good safety and gentleness.

[0017] As a preferred embodiment of the massage oil of the present invention, the massage oil comprises the following components in weight percentage: 9.5%-18% of the plant oil essential oil composition, 60%-75% of the base oil, 5%-10% of the blending oil, 1%-2.5% of the conditioning agent, 0.2%-1% of the antioxidant, 0.05%-0.3% of the soothing agent, 0.03%-0.1% of the warming agent, and the balance being the solvent.

[0018] Preferably, the massage oil comprises the following components by weight percentage: 9.5%-18% of the plant oil essential oil composition, 66%-69% of the base oil, 5.98%-8.35% of the blending oil, 1.61%-2.05% of the conditioning agent, 0.2%-0.8% of the antioxidant, 0.1%-0.27% of the soothing agent, 0.03%-0.1% of the warming agent, and the balance being the solvent.

[0019] More preferably, the massage oil comprises the following components by weight percentage: 13.3% of the plant oil essential oil composition, 67.17% of the base oil, 6.24% of the blending oil, 1.77% of the conditioning agent, 0.46% of the antioxidant, 0.18% of the soothing agent, 0.06% of the warming agent, and the balance being the solvent.

[0020] As a preferred embodiment of the massage oil of the present invention, the base oil includes at least one of sunflower seed oil, olive fruit oil, European hazelnut seed oil, and macadamia seed oil; the blending oil includes at least one of lemon peel oil, mugwort oil, magnolia leaf oil, bergamot fruit oil, ginger root oil, Angelica sinensis root oil, Ligusticum chuanxiong oil, and geranium oil; the conditioning agent includes at least one of Liquidambar formosana extract, Spatholobus suberectus extract, Clematis chinensis extract, and Panax notoginseng root extract; and the soothing agent includes Glycyrrhiza glabra root extract and / or stearyl glycyrrhizate. Preferably, the base oils include sunflower seed oil, olive fruit oil, European hazelnut seed oil, and macadamia seed oil; the blending oils include lemon peel oil, mugwort oil, magnolia leaf oil, bergamot fruit oil, ginger root oil, Angelica sinensis root oil, Ligusticum chuanxiong oil, and geranium oil; the conditioning agents include Liquidambar formosana extract, Spatholobus suberectus extract, Clematis chinensis extract, and Panax notoginseng root extract; and the soothing agents include Glycyrrhiza glabra root extract and stearyl glycyrrhizic acid ester.

[0021] In a preferred embodiment of the massage oil of the present invention, the antioxidant includes tocopheryl acetate; the heat-sensing agent includes vanillyl butyl ether; and the solvent includes squalane.

[0022] Fourthly, the present invention provides a method for preparing the massage oil, comprising the following steps:

[0023] (1) Mix the plant oil essential oil composition, base oil and solvent evenly to obtain mixture A;

[0024] (2) Mix the blended oil and the conditioning agent evenly to obtain mixture B;

[0025] (3) Mix the antioxidant, soothing agent and heat-sensing agent evenly to obtain mixture C;

[0026] (4) Mix the mixture A, mixture B and mixture C, stir, and let stand to obtain the final product.

[0027] The method for preparing massage oil according to the present invention has mild reaction conditions, simple operation steps, and is easy to scale up for industrial production.

[0028] In a preferred embodiment of the method for preparing the massage oil of the present invention, the stirring time is 2h-5h; the settling time is 10h-15h.

[0029] Fifthly, the present invention provides the application of the aforementioned plant oil and essential oil composition and the aforementioned massage oil in skin care products and health products.

[0030] Compared with the prior art, the beneficial effects of the present invention are as follows: First, the plant oil essential oil composition of the present invention achieves significant anti-inflammatory, soothing, anti-wrinkle, and antioxidant effects through the synergistic effect of multiple active ingredients. Moreover, its active ingredients are all natural plant oils or plant oil extracts, avoiding the use of chemically synthesized substances, reducing the risk of irritation and side effects to the skin and body. It is suitable for various needs such as mood management, pain relief, strengthening tendons and ligaments, promoting blood circulation and removing blood stasis, and skin care, and has good safety and gentleness. Secondly, the massage oil prepared by adding the plant oil and essential oil composition of the present invention to the massage oil can penetrate deep into the skin, inhibit the production and release of inflammatory factors, reduce skin inflammation, and soothe symptoms such as redness, swelling, pain, and itching caused by inflammation, promoting skin repair and regeneration; balancing the skin's oil secretion, relieving skin sensitivity and discomfort, promoting blood circulation, and eliminating fatigue; promoting collagen synthesis and elastin fiber formation, reducing the formation of fine lines and wrinkles, and increasing skin elasticity and radiance; scavenging free radicals, protecting the skin from oxidative damage, delaying the skin aging process, reducing the formation of age spots and dullness, and making the skin brighter and more even-toned. Furthermore, the preparation method of the massage oil of the present invention uses mild reaction conditions, simple operation steps, and is easy to scale up for industrial production. Detailed Implementation

[0031] To better illustrate the objectives, technical solutions, and advantages of this invention, the invention will be further described below with reference to specific embodiments. Those skilled in the art should understand that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.

[0032] The following description, in conjunction with specific embodiments, illustrates the practical effects of the present invention.

[0033] Unless otherwise specified, the experimental methods used in the examples are conventional methods; the materials, reagents, equipment, etc. used are all commercially available unless otherwise specified.

[0034] The raw materials used in the following embodiments and comparative examples are described below, but are not limited to these materials:

[0035] The deep-sea two-jointed shepherd's purse seed oil, rust-red rose seed oil, evening primrose oil, sunflower seed oil, olive fruit oil, European hazelnut seed oil and macadamia seed oil were all purchased from Northstar Lipids (UK) Ltd.

[0036] The following extracts were purchased from Guangzhou Xinsimei Biotechnology Co., Ltd.: root bark extract of Acanthopanax gracilis, flower extract of Jasminum sambac, lemon peel oil, Artemisia argyi oil, Magnolia officinalis leaf oil, bergamot fruit oil, ginger root oil, Angelica sinensis root oil, Ligusticum chuanxiong oil, Geranium geranium oil, Liquidambar formosana extract, Spatholobus suberectus extract, Phryma leptostachya extract, Panax notoginseng root extract, Borage seed oil, Hippophae rhamnoides fruit oil, Lithospermum erythrorhizon oil, Acanthopanax senticosus root extract, and Jasminum sambacini extract.

[0037] The tocopherol acetate was purchased from BASF (China) Co. Ltd. Shanghai (BASF, Germany);

[0038] The licorice root extract, stearyl glycyrrhizate, and vanillyl butyl ether used were purchased from Xinjiang Caozimu Pharmaceutical Co., Ltd.

[0039] In the following examples and comparative examples, the preparation method of the plant oil essential oil composition involved is as follows: the deep-sea two-section shepherd's purse seed oil, rust-red rose seed oil, evening primrose oil, eleutherococcus root bark extract and jasmine flower extract are mixed evenly to obtain the composition.

[0040] The raw materials (by weight) for the following examples and comparative vegetable oil essential oil compositions are shown in Tables 1 and 2.

[0041] Table 1. Raw material ratios (parts by weight) of the plant oil and essential oil compositions in the embodiments of the present invention.

[0042] Components Deep-sea two-section shepherd's purse oil Rust-red Rose Seed Oil Evening Primrose Oil Root bark extract of Acanthopanax senticosus Jasmine flower extract Example 1 56 17 15 8 4 Example 2 60.96 15.5 13.57 6.85 3.12 Example 3 67.14 13.16 11.28 5.64 2.78 Example 4 78.8 9 8 3 1.2 Example 5 69.5 10 10 7.5 3 Example 6 80 5 11 2 2 Example 7 50 20 10 15 2

[0043] Table 2. Raw material ratios (parts by weight) of the comparative plant oil and essential oil compositions of the present invention.

[0044]

[0045]

[0046] The massage oil preparation methods involved in the following application examples and comparative examples are as follows:

[0047] (1) Mix sunflower seed oil, olive fruit oil, European hazelnut seed oil, macadamia seed oil, vegetable oil essential oil composition and squalane evenly to obtain mixture A;

[0048] (2) Mix lemon peel oil, mugwort oil, magnolia leaf oil, bergamot fruit oil, ginger root oil, round-leaf angelica root oil, chuanxiong oil, geranium oil, and extracts of luffa, chicken blood vine, clematis, and notoginseng root to obtain mixture B.

[0049] (3) Mix tocopheryl acetate, stearyl glycyrrhetinic acid ester, licorice root extract and vanillyl butyl ether evenly to obtain mixture C;

[0050] (4) Mix mixture A, mixture B and mixture C, stir at 200 rpm for 3 hours and let stand for 12 hours to obtain massage oil.

[0051] The following application examples and comparative examples of massage oil ingredients (by weight percentage) are shown in Table 3.

[0052] Table 3: Raw material ratios (by weight percentage) of massage oils used in application examples and comparative examples of this invention.

[0053]

[0054]

[0055] Test Example 1: Anti-inflammatory Effect Test

[0056] (1) Materials and methods:

[0057] (I) Test samples: First, the vegetable oil essential oil compositions of Examples 1-7 and Comparative Examples 1-12 were prepared into a solution with a mass concentration of 30% using dimethyl sulfoxide, and then a solution with a mass concentration of 1% was prepared using DMEM-H complete culture medium.

[0058] (II) Test Principle: Rheumatoid arthritis is an autoimmune disease characterized by chronic inflammation of the synovium. Untreated rheumatoid arthritis leads to persistent joint destruction, resulting in disability, low quality of life, and increased mortality. Fibroblast-like synovial cells (FLS) and inflammatory cells, such as macrophages and T cells, produce pro-inflammatory cytokines, such as IL-1β and TNF-α, which play a key role in the pathogenesis of rheumatoid arthritis. The test examples of this invention use a human rheumatoid arthritis fibroblast-like synovial cell (RA-FLS) model to evaluate the inhibitory effect of the plant oil essential oil composition of this invention on IL-1β.

[0059] (III) Test Procedure: RA-FLS cells in the logarithmic growth phase (purchased from: Cell Resource Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences) were added to 6-well culture plates, with 2 × 10⁶ cells added to each well. 5 Cells were cultured in DMEM-H complete medium at 37°C, 5% CO2, and saturated humidity for 24 h. The medium was then discarded. 1 mL of DMEM-H complete medium was added to the blank control group and model group, and 1 mL of the corresponding test sample was added to the sample group. Cells were cultured again at 37°C, 5% CO2, and saturated humidity for 24 h. Subsequently, cells in the model group and sample group were stimulated with 1 mL of 10 ng / mL lipopolysaccharide (LPS) for 6 h. The supernatant and cell lysate were collected. IL-1β in the cell culture supernatant was quantified using an IL-1β enzyme-linked immunosorbent assay (ELISA) kit. The IL-1β content was measured at 450 nm using a microplate reader. The IL-1β content in the blank control group was taken as 1.00, and the relative IL-1β content in other groups was calculated.

[0060] (2) Test Results: As shown in Table 4, the cells in the model group, without using any test sample, showed a significant increase in IL-1β levels after LPS stimulation, which was 1.83 times that of the blank control group. However, RA-FLS cells treated with the test sample showed varying degrees of decrease in IL-1β levels after LPS stimulation, indicating that the test sample could inhibit IL-1β expression in RA-FLS cells, demonstrating its excellent anti-inflammatory effect. The plant oil essential oil composition in the examples showed IL-1β expression levels comparable to, or even lower than, those in the blank control group.

[0061] Test Example 2: Soothing Efficacy Test

[0062] (1) Materials and methods:

[0063] (I) Test samples: First, the vegetable oil essential oil compositions of Examples 1-7 and Comparative Examples 1-12 were prepared into a solution with a mass concentration of 30% using dimethyl sulfoxide, and then a solution with a mass concentration of 1% was prepared using deionized water.

[0064] (II) Test Principle: Hyaluronidase is involved in type I hypersensitivity reactions and is strongly correlated with inflammation and allergies. This test demonstrates its soothing effect by testing the inhibitory effect of the sample on hyaluronidase activity.

[0065] (III) Test procedure: The hyaluronidase inhibition rate of each test sample was calculated by the Elson-Morgan hyaluronidase inhibition experiment.

[0066] Four groups (A, B, C, and D) are set up, and each group has three parallel subgroups.

[0067] Group A is a reaction solution containing the sample and enzyme: 0.1 mL of 2.5 mmol / L calcium chloride solution, 0.5 mL of 600 U / mL hyaluronidase, 0.1 mL of acetate buffer solution, 0.5 mL of sample solution, 0.5 mL of 0.4 mol / L sodium hydroxide, 0.5 mL of acetone-acetic acid solution, and 1 mL of Ehrlich reagent are added sequentially to the test tube.

[0068] Group B consists of reaction solutions containing the sample and those without the enzyme: 0.1 mL of 2.5 mmol / L calcium chloride solution, 0.5 mL of deionized water, 0.1 mL of acetate buffer solution, 0.5 mL of sample solution, 0.5 mL of 0.4 mol / L sodium hydroxide, 0.5 mL of acetone-acetic acid solution, and 1 mL of Ehrlich reagent were added sequentially to the test tube.

[0069] Group C is a reaction solution containing enzyme but without sample: 0.1 mL of 2.5 mmol / L calcium chloride solution, 0.5 mL of 600 U / mL hyaluronidase, 0.1 mL of acetate buffer solution, 0.5 mL of deionized water, 0.5 mL of 0.4 mol / L sodium hydroxide, 0.5 mL of acetone-acetic acid solution, and 1 mL of Ehrlich reagent were added to the test tube in sequence.

[0070] Group D is a reaction solution that does not contain the sample or enzyme: 0.1 mL of 2.5 mmol / L calcium chloride solution, 1 mL of deionized water, 0.1 mL of acetate buffer solution, 0.5 mL of 0.4 mol / L sodium hydroxide, 0.5 mL of acetone-acetic acid solution, and 1 mL of Ehrlich reagent are added to the test tube in sequence.

[0071] After mixing the four reaction solutions, the solutions were successively placed in boiling water, ice water, and room temperature environments for reaction. P-DAB colorimetric reagent was then added to each of the four tubes. After the solutions had completely reacted, the absorbance of each group was measured at a wavelength of 530 nm. The inhibition rate of hyaluronidase was calculated based on the absorbance of each group.

[0072] The formula for calculating the inhibition rate of hyaluronidase is as follows:

[0073]

[0074] Where A is the absorbance of the reaction solution containing the sample and enzyme; B is the absorbance of the reaction solution containing the sample and without the enzyme; C is the absorbance of the reaction solution containing the enzyme but without the sample; and D is the absorbance of the reaction solution without the sample and enzyme.

[0075] (2) Test results: As shown in Table 4, the test results show that the plant oil essential oil composition of the present invention can effectively inhibit the activity of hyaluronidase, indicating that the plant oil essential oil composition of the present invention has a good soothing effect.

[0076] Test Example 3: Anti-wrinkle performance test

[0077] (1) Materials and methods:

[0078] (I) Test samples: First, the vegetable oil essential oil compositions of Examples 1-7 and Comparative Examples 1-12 were prepared into a solution with a mass concentration of 30% using dimethyl sulfoxide, and then a solution with a mass concentration of 1% was prepared using deionized water.

[0079] (II) Test principle: Elastase is the most important enzyme in the matrix metalloproteinase family. It is mainly synthesized and secreted by fibroblasts and can degrade elastin in the skin, leading to skin aging. This test case characterizes the anti-wrinkle and firming effects by evaluating the inhibition rate of elastase in the test sample.

[0080] (III) Test procedure: Prepare Tricine buffer (50 mmol / L, pH 7.5) containing 400 mmol / L NaCl and 10 mmol / L CaCl2. Use Tricine buffer to prepare 0.8 U / mL elastase solution and 2 mmol / L N-[3-(2-furanyl)acryloyl]-leucine-glycine-proline-alanine (FALGPA) solution.

[0081] Group A was the test group: 40 μL of sample solution was mixed with 100 μL of Tricine buffer, and then 20 μL of 0.8 U / mL elastase solution was added. After mixing, the mixture was incubated at 25 °C for 15 min. Then, 40 μL of 2 mmol / L FALGPA solution was added. After 15 min, the absorbance value was measured at 335 nm. The measurements were repeated in triplicate.

[0082] Group B is a reaction solution containing the sample but without enzyme: 40 μL of sample solution is mixed with 100 μL of Tricine buffer, and then 20 μL of 0.8 U / mL elastase solution is added. After mixing, the mixture is incubated at 25 °C for 15 min, and then 40 μL of 2 mmol / L FALGPA solution is added. After 15 min, the absorbance value is measured at 335 nm. The measurement is repeated in triplicate.

[0083] Group C is a reaction solution containing enzymes but without samples: 40 μL of purified water and 100 μL of Tricine buffer were mixed, and then 20 μL of 0.8 U / mL elastase solution was added. After mixing, the mixture was incubated at 25 °C for 15 min. Then, 40 μL of 2 mmol / L FALGPA solution was added. After 15 min, the absorbance value was measured at 335 nm. The measurement was repeated in triplicate.

[0084] Group D is the reaction solution without sample and enzyme: 40 μL of purified water is mixed with 100 μL of Tricine buffer, then 20 μL of purified water is added. After mixing, it is incubated at 25℃ for 15 min, then 40 μL of 2 mmol / L FALGPA solution is added. After 15 min, the absorbance value is measured at 335 nm. The measurement is repeated in triplicate.

[0085] The inhibition rate of the sample against elastase was calculated using the following formula:

[0086]

[0087] Where A is the absorbance of the test group; B is the absorbance of the reaction solution containing the sample but without the enzyme; C is the absorbance of the reaction solution containing the enzyme but without the sample; and D is the absorbance of the reaction solution without both the sample and the enzyme.

[0088] (2) Test results: As shown in Table 4, the plant oil essential oil composition of the present invention can effectively inhibit the activity of elastase, indicating that the plant oil essential oil composition of the present invention has a good anti-wrinkle effect.

[0089] Test Example 4: Antioxidant Performance Test

[0090] (1) Materials and methods:

[0091] (I) Test samples: First, the vegetable oil essential oil compositions of Examples 1-7 and Comparative Examples 1-12 were prepared into a solution with a mass concentration of 30% using dimethyl sulfoxide, and then a solution with a mass concentration of 1% was prepared using deionized water.

[0092] (II) Test Procedure: Take 5 mL of Tris-HCl buffer solution with pH 8.2 into a stoppered test tube, preheat in a 25°C water bath for 20 min, then add 2 mL of sample solution and 5 × 10⁻⁶ ppm of HCl solution sequentially. -3 0.5 mL of pyrogallol solution (mol / L) (using 1×10⁻⁶) -2 Prepared with mol / L HCl solution, immediately shaken and reacted in a 25℃ water bath for 4 min, then immediately measured its absorbance A1 at 320 nm; using 1×10 -2 0.5 mL of mol / L HCl solution was used to replace the pyrogallol solution for absorbance measurement A2; 2 mL of pure water was used to replace the sample solution for absorbance measurement A3. The absorbance A2 was calculated using the following formula. - The clearance rate.

[0093]

[0094] Where A1 is the absorbance of the solution after adding the sample to scavenge free radicals; A3 is the absorbance of the solution without the sample; and A2 is the absorbance of the sample at the measurement wavelength.

[0095] (2) Test results: As shown in Table 4, the plant oil essential oil composition of the present invention can eliminate O2. - It exhibits excellent antioxidant effects against free radicals.

[0096] Table 4: Relative IL-1β content in test group cells of the examples and comparative examples

[0097]

[0098]

[0099] As can be seen from the results in Table 4, the effects of the plant oil and essential oil compositions in Examples 1-7 of the present invention are significantly better than those in Comparative Examples 1-12. Specifically, the synergistic effect among the plant oil and essential oil compositions of the present invention—namely, the oils of *Capsella bursa-pastoris* seed, *Rosa rubra* seed, evening primrose oil, *Eleutherococcus senticosus* root bark extract, and *Jasminum nudiflorum* flower extract—can effectively inhibit IL-1β expression in RA-FLS cells, inhibit hyaluronidase activity, inhibit elastase activity, and eliminate ·O2. 2- Free radicals have excellent anti-inflammatory, soothing, anti-wrinkle, and antioxidant effects. If one of the components is missing or the component is replaced, the anti-inflammatory, soothing, anti-wrinkle, and antioxidant effects of the composition will be reduced.

[0100] Test Example 5: Massage Oil User Experience and Effect Test

[0101] (1) Test samples: Massage oils used in Examples 1-4 and Comparative Examples 1 and 2;

[0102] (2) Testing Procedure: Sixty volunteers aged 40-60 years with arthritis were selected (one test sample was used for every 10 volunteers). Volunteers applied an appropriate amount of massage oil to the affected areas of arthritis, rubbed for 2 minutes, and used the product continuously for 2 weeks, once daily. Volunteers evaluated the user experience and efficacy of the sample after use. The scoring criteria were: 0 points for no effect and 10 points for excellent effect. Scores could be any value between 0 and 10. The average test results were taken.

[0103] (3) The test results are shown in Table 6. The test results show that after using the massage oil of the present invention for two weeks, the pain of arthritis in volunteers was well relieved, the fatigue was well eliminated, and the skin of the corresponding area improved after two weeks of use. This shows that the massage oil provided by the present invention can not only strengthen the tendons and ligaments and promote blood circulation, but also has the effect of skin care.

[0104] Table 6: Evaluation Table of Massage Oil User Experience and Efficacy in Application Examples and Comparative Examples

[0105]

[0106] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A plant oil essential oil composition, characterized in that, The product comprises the following components in parts by weight: 60.96-67.14 parts of deep-sea shepherd's purse seed oil, 13.16-15.5 parts of rust-red rose seed oil, 11.28-13.57 parts of evening primrose oil, 5.64-6.85 parts of Acanthopanax senticosus root bark extract, and 2.78-3.12 parts of jasmine flower extract; the mass ratio of evening primrose oil to Acanthopanax senticosus root bark extract is 1:(0.5-0.525); both Acanthopanax senticosus root bark extract and jasmine flower extract were purchased from Guangzhou Xinsimei Biotechnology Co., Ltd.

2. The method for preparing the plant oil essential oil composition according to claim 1, characterized in that, Includes the following steps: The deep-sea two-section shepherd's purse seed oil, rust-red rose seed oil, evening primrose oil, eleutherococcus root bark extract and jasmine flower extract are mixed evenly to obtain the product.

3. A massage oil, characterized in that, The massage oil comprises the plant oil essential oil composition of claim 1.

4. The massage oil as described in claim 3, characterized in that, It comprises the following components by weight percentage: 9.5%-18% of the plant oil essential oil composition, 60%-75% of the base oil, 5%-10% of the blending oil, 1%-2.5% of the conditioning agent, 0.2%-1% of the antioxidant, 0.05%-0.3% of the soothing agent, 0.03%-0.1% of the heat-sensing agent, and the balance being the solvent.

5. The massage oil as described in claim 4, characterized in that, The base oil includes at least one of sunflower seed oil, olive fruit oil, European hazelnut seed oil, and macadamia seed oil; the blending oil includes at least one of lemon peel oil, mugwort oil, magnolia leaf oil, bergamot fruit oil, ginger root oil, round-leaf angelica root oil, chuanxiong oil, and geranium oil; the conditioning agent includes at least one of luffa extract, chicken blood vine extract, clematis extract, and notoginseng root extract; the soothing agent includes licorice root extract and / or stearyl glycyrrhizate.

6. The method for preparing the massage oil according to any one of claims 3-5, characterized in that, Includes the following steps: (1) The plant oil essential oil composition, base oil, and solvent are mixed evenly to obtain mixture A; (2) Mix the blended oil and the conditioning agent evenly to obtain mixture B; (3) Mix the antioxidant, soothing agent and heat-sensing agent evenly to obtain mixture C; (4) Mix the mixture A, mixture B and mixture C, stir, and let stand to obtain the final product.

7. The method for preparing massage oil as described in claim 6, characterized in that, The stirring time is 2-5 hours; the settling time is 10-15 hours.

8. The use of the plant oil essential oil composition according to claim 1 and the massage oil according to any one of claims 3-5 in skin care products and health products.

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