Leukocyte extraction device and method for inhibiting expression of proinflammatory factors

Abstract

The invention belongs to the technical field of cell extraction, and particularly relates to a leukocyte extraction device and method for inhibiting proinflammatory factor expression, the leukocyte extraction device comprises a centrifugal container and a control mainboard arranged on one side of the centrifugal container, the leukocyte extraction device further comprises an outer sleeve, the outer sleeve is connected to the outer side of the centrifugal container in a sleeving mode, and two mounting grooves are formed in the inner wall of the outer sleeve; the two mounting grooves are symmetrical about the axis of the centrifugal container, and a light detection mechanism is jointly mounted in the two mounting grooves; the protruding part is integrally formed and arranged on the upper side of the outer side wall of the outer sleeve, and a circular cavity is formed in the protruding part. According to the method, the layering condition of the blood sample can be automatically judged, and after the layering of the blood sample is stable, personnel are automatically reminded, so that unnecessary time waste can be reduced and the leukocyte extraction efficiency can be improved under the condition of ensuring full separation of leukocytes, and low temperature can be kept during centrifugation, thereby being beneficial to separation and extraction of leukocytes.

Description

Technical Field

[0001] The present invention belongs to the technical field of cell extraction, and in particular relates to a leukocyte extraction device and extraction method for inhibiting the expression of pro-inflammatory factors. Background Art

[0002] In inflammation-related research and treatment, inhibiting the expression of pro-inflammatory factors is crucial. As immune cells, leukocytes have potential value in inhibiting the expression of pro-inflammatory factors in their extracts, which can be used for developing anti-inflammatory drugs, exploring the mechanisms of inflammation regulation, and opening up new paths for the treatment of inflammatory diseases.

[0003] Currently, for leukocyte extraction, blood samples are generally collected first. After anticoagulation, dilution and other treatments, centrifugation equipment is used to stratify cells of different densities, so as to separate and extract leukocytes. The samples generally need to be loaded into centrifugation containers after being processed, and then installed on a centrifuge for centrifugation treatment. For example, a method and device for extracting high-purity leukocytes disclosed in the patent publication number CN108342357B; During the leukocyte extraction process, in order to improve efficiency, multiple samples are usually processed at one time during centrifugation. However, each sample has individual differences, which leads to different centrifugation times required for separating their respective leukocytes. Currently, the commonly used method is to centrifuge uniformly for about 30 minutes. Although this way of extending the centrifugation time can ensure sufficient centrifugation separation of the samples to a certain extent, it undoubtedly causes unnecessary time waste and has a negative impact on the overall leukocyte extraction efficiency. On the contrary, if the centrifugation time is too short, there is a high probability that some samples will not be centrifuged sufficiently, thereby reducing the extraction rate of leukocytes. Summary of the Invention

[0004] The purpose of the present invention is to solve the above problems and provide a leukocyte extraction device and extraction method for inhibiting the expression of pro-inflammatory factors.

[0005] To achieve the above purpose, the present invention adopts the following technical solutions: A leukocyte extraction device for inhibiting the expression of pro-inflammatory factors, including a centrifugation container and a control main board arranged on one side of the centrifugation container, and further includes: A jacket, sleeved on the outside of the centrifugation container. Two installation grooves are provided on the inner wall of the jacket, and the two installation grooves are symmetric about the axis of the centrifugation container. A light detection mechanism is jointly installed in the two installation grooves; A protruding part, integrally formed on the upper side of the outer side wall of the jacket. A circular cavity is provided inside the protruding part, and a compression driving mechanism is arranged inside the circular cavity. The light detection mechanism is electrically connected to the compression driving mechanism; A heat preservation unit, arranged inside the jacket. The compression driving mechanism squeezes the air inside the circular cavity into the inside of the heat preservation unit; An exhaust groove is formed on the outer side wall of the outer sleeve, and the exhaust groove is communicated with the air outlet end of the heat preservation unit. A wind blocking component and a triggering component are installed inside the exhaust groove. A timing module is arranged on the control main board, and the triggering component is electrically connected to the timing module through the control main board.

[0006] Preferably, the light detection mechanism includes a strip-shaped lamp board and a photosensitive resistor strip. The strip-shaped lamp board and the photosensitive resistor strip are both installed inside the installation groove on the same side. The light emitted by the strip-shaped lamp board passes through the centrifuge container and projects onto the photosensitive surface of the photosensitive resistor strip. The strip-shaped lamp board is electrically connected to the control main board.

[0007] Preferably, the compression driving mechanism includes a piston slidably arranged inside the circular cavity. A non-magnetic elastic member is fixedly arranged between the side wall of the piston and the circular cavity. An electromagnetic block is installed at a position on the cavity wall of the circular cavity inside the non-magnetic elastic member. A permanent magnet is fixedly installed on the side wall of the piston close to the electromagnetic block. The electromagnetic block is electrically connected to the control main board through the photosensitive resistor strip.

[0008] Preferably, the heat preservation unit includes an air inlet hole and an air outlet hole formed on the cavity wall of the circular cavity. Check valves are installed inside both the air inlet hole and the air outlet hole. An arc-shaped air inlet groove and an arc-shaped air outlet groove are formed on the inner wall of the outer sleeve, and the arc-shaped air inlet groove and the arc-shaped air outlet groove are symmetrically arranged on both sides of the centrifuge container. Two communication grooves are formed on the lower side wall of the arc-shaped air inlet groove and the arc-shaped air outlet groove. The air outlet hole is communicated with the arc-shaped air inlet groove. An exhaust hole is formed on the groove wall of the arc-shaped air outlet groove, and the exhaust hole is communicated with the exhaust groove.

[0009] Preferably, the wind blocking component includes a reflective film movably arranged inside the exhaust groove. A pressing strip is fixedly installed on one side wall of the reflective film, and the pressing strip is fixedly connected to the bottom of the exhaust groove. The side wall of the reflective film is in contact with the bottom of the exhaust groove, and the air flow discharged from the exhaust hole will blow onto the side wall of the reflective film.

[0010] Preferably, the triggering component includes a light guide column arranged on one side of the strip-shaped lamp board. One end of the light guide column is arranged inside the installation groove on the same side, and the other end of the light guide column penetrates through the bottom of the exhaust groove. A fixing groove is formed on the bottom of the exhaust groove, and a photodiode is installed inside the fixing groove. The light emitted by the strip-shaped lamp board is conducted through the light guide column and irradiates onto the side wall of the reflective film. The photodiode is electrically connected to the timing module through the control main board.

[0011] Preferably, a semiconductor refrigerator is fixedly inserted into the side wall of the circular cavity away from the piston, and the refrigerating end of the semiconductor refrigerator is arranged inside the circular cavity. The semiconductor refrigerator is electrically connected to the control main board.

[0012] An extraction method for a leukocyte extraction device that inhibits the expression of pro-inflammatory factors as described above, the extraction method comprising the following steps: S1. Collect an appropriate amount of blood sample using a sterile blood collection needle and immediately inject it into a blood collection tube containing an anticoagulant, gently invert and mix to prevent blood coagulation; S2. Add a diluent to the blood sample in a certain proportion, gently shake well, fully dilute, and pour the diluted blood sample into a centrifuge container; S3. Install the centrifuge containers containing blood samples on the turntable of the centrifuge equipment in sequence, and connect the cable connectors on each centrifuge container to the interfaces of the control main board; S4. Centrifuge the centrifuge containers at a speed of 1800 - 2000 revolutions per minute through the centrifuge equipment. At the same time, start the control main board. After the control main board is started, it will control the light detection mechanism, compression drive mechanism, trigger component, and timing module to work; S5. After the control main board receives the electrical signal fed back by the timing module, the control main board sends a prompt voice to the staff, and the staff can then shut down the centrifuge equipment; S6. Separate the cable connectors of each centrifuge container from the control main board, gently take out each centrifuge container, and then use a pipette to transfer the leukocyte layer in each centrifuge container to a sterile tube.

[0013] Compared with the existing technology, the advantages of a leukocyte extraction device and an extraction method for inhibiting the expression of pro-inflammatory factors are as follows: 1. Through the provided centrifuge container, it can hold the blood sample. Through the mutual cooperation of the provided control main board, outer casing, installation groove, light detection mechanism, protruding part, circular cavity, compression drive mechanism, heat preservation unit, exhaust groove, wind shield component, trigger component, and timing module, during the process of centrifuging and extracting the blood sample, based on the stability of the light change, it can automatically determine whether the blood sample is fully stratified, thereby reducing unnecessary time waste while ensuring the full separation of leukocytes and improving the extraction efficiency of leukocytes.

[0014] 2. Through the provided heat preservation unit, in cooperation with the provided semiconductor refrigerator, it can keep the inside of the centrifuge container at a low temperature during the process of centrifuging and separating the blood sample, which can facilitate the subsequent extraction of the leukocyte layer and maintain the stability of leukocytes as much as possible, indirectly improving the accuracy of subsequent leukocyte detection and analysis.

[0015] 3. Through the provided wind shield component, trigger component, and timing module, it can utilize the airflow generated by the operation of the heat preservation unit and the light generated by the light detection mechanism to automatically trigger the timing module to feedback an electrical signal after the blood sample is stably stratified, reducing the possibility of false triggering. Description of the Drawings

[0016] Figure 1 is a schematic structural diagram of a leukocyte extraction device for inhibiting the expression of pro-inflammatory factors provided by the present invention; Figure 2 is a schematic cross-sectional structural diagram of a leukocyte extraction device for inhibiting the expression of pro-inflammatory factors provided by the present invention; Figure 3 is a schematic top view structural diagram of a leukocyte extraction device for inhibiting the expression of pro-inflammatory factors provided by the present invention; Figure 4 is a schematic cross-sectional structural diagram of an optical inspection mechanism of a leukocyte extraction device for inhibiting the expression of pro-inflammatory factors provided by the present invention; Figure 5 is a schematic internal structural diagram of a circular cavity of a leukocyte extraction device for inhibiting the expression of pro-inflammatory factors provided by the present invention; Figure 6 is a schematic connection structural diagram of an arc-shaped air inlet groove and an arc-shaped air outlet groove of a leukocyte extraction device for inhibiting the expression of pro-inflammatory factors provided by the present invention; Figure 7 is a leukocyte extraction device for inhibiting the expression of pro-inflammatory factors provided by the present invention Figure 3 and is an enlarged structural view of part A therein; Figure 8 is a three-dimensional structural diagram of a reflective film of a leukocyte extraction device for inhibiting the expression of pro-inflammatory factors provided by the present invention.

[0017] In the figure: 1 centrifugal container, 2 control main board, 3 outer casing, 4 installation groove, 5 optical inspection mechanism, 51 strip-shaped lamp board, 52 photosensitive resistor strip, 6 protruding part, 7 circular cavity, 8 compression driving mechanism, 81 piston, 82 non-magnetic elastic member, 83 electromagnetic block, 84 permanent magnet, 9 heat preservation unit, 91 air inlet hole, 92 air outlet hole, 93 one-way valve, 94 arc-shaped air inlet groove, 95 arc-shaped air outlet groove, 96 communication groove, 97 exhaust hole, 10 exhaust groove, 11 wind shielding component, 111 reflective film, 112 pressing strip, 12 trigger component, 121 light guide column, 122 fixing groove, 123 photodiode, 13 timing module, 14 semiconductor refrigerator. Specific embodiments

[0018] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all of the embodiments.

[0019] Such as Figures 1-8As shown in the figure, a leukocyte extraction device for inhibiting the expression of pro-inflammatory factors includes a centrifugation container 1 and a control main board 2 arranged on one side of the centrifugation container 1. It further includes: an outer sleeve 3, which is sleeved on the outside of the centrifugation container 1. Two installation grooves 4 are opened on the inner wall of the outer sleeve 3, and the two installation grooves 4 are symmetric about the axis of the centrifugation container 1. A light detection mechanism 5 is jointly installed in the two installation grooves 4. The light detection mechanism 5 includes a strip-shaped lamp board 51 and a photosensitive resistor strip 52. The strip-shaped lamp board 51 and the photosensitive resistor strip 52 are both installed inside the installation groove 4 on the same side. The light emitted by the strip-shaped lamp board 51 passes through the centrifugation container 1 and projects onto the photosensitive surface of the photosensitive resistor strip 52. The strip-shaped lamp board 51 is electrically connected to the control main board 2. Within a certain range of light intensity, the resistance of the photosensitive resistor strip 52 decreases as the light intensity increases.

[0020] A protruding portion 6 is integrally formed on the upper side of the outer wall of the outer sleeve 3. A circular cavity 7 is opened inside the protruding portion 6, and a compression driving mechanism 8 is arranged inside the circular cavity 7. The light detection mechanism 5 is electrically connected to the compression driving mechanism 8. The compression driving mechanism 8 includes a piston 81 slidably arranged inside the circular cavity 7. A non-magnetic elastic member 82 is fixedly arranged between the side wall of the piston 81 and the circular cavity 7. An electromagnetic block 83 is installed on the cavity wall of the circular cavity 7 at a position inside the non-magnetic elastic member 82. A permanent magnet 84 is fixedly installed on the side wall of the piston 81 close to the electromagnetic block 83. The electromagnetic block 83 is electrically connected to the control main board 2 through the photosensitive resistor strip 52. After the electromagnetic block 83 is energized, it will generate a magnetic repulsive force on the permanent magnet 84.

[0021] A heat preservation unit 9 is arranged inside the outer sleeve 3. The compression driving mechanism 8 squeezes the air inside the circular cavity 7 into the inside of the heat preservation unit 9. The heat preservation unit 9 includes an air inlet hole 91 and an air outlet hole 92 opened on the cavity wall of the circular cavity 7. Check valves 93 are installed inside both the air inlet hole 91 and the air outlet hole 92. An arc-shaped air inlet groove 94 and an arc-shaped air outlet groove 95 are opened on the inner wall of the outer sleeve 3, and the arc-shaped air inlet groove 94 and the arc-shaped air outlet groove 95 are symmetrically arranged on both sides of the centrifugation container 1. Two communication grooves 96 are jointly opened on the lower side wall of the arc-shaped air inlet groove 94 and the arc-shaped air outlet groove 95. The air outlet hole 92 is communicated with the arc-shaped air inlet groove 94. An exhaust hole 97 is opened on the groove wall of the arc-shaped air outlet groove 95, and the exhaust hole 97 is communicated with the exhaust groove 10.

[0022] The exhaust groove 10 is opened on the outer side wall of the outer sleeve 3, and the exhaust groove 10 is communicated with the air outlet end of the heat preservation unit 9. A wind blocking component 11 and a triggering component 12 are installed inside the exhaust groove 10. A timing module 13 is arranged on the control main board 2. The triggering component 12 is electrically connected to the timing module 13 through the control main board 2. The wind blocking component 11 includes a reflective film 111 movably arranged inside the exhaust groove 10. A pressing strip 112 is fixedly installed on one side wall of the reflective film 111, and the pressing strip 112 is fixedly connected to the bottom of the exhaust groove 10. The side wall of the reflective film 111 is in contact with the bottom of the exhaust groove 10, and the air flow discharged from the exhaust hole 97 will be blown to the side wall of the reflective film 111. The pressing strip 112 can fix one end of the reflective film 111 at the bottom of the exhaust groove 10.

[0023] The triggering component 12 includes a light guide column 121 arranged on one side of the strip-shaped lamp board 51. One end of the light guide column 121 is arranged in the installation groove 4 on the same side, and the other end of the light guide column 121 penetrates through the bottom of the exhaust groove 10. A fixing groove 122 is opened at the bottom of the exhaust groove 10, and a photodiode 123 is installed inside the fixing groove 122. The light emitted by the strip-shaped lamp board 51 is conducted through the light guide column 121 and irradiated to the side wall of the reflective film 111. The photodiode 123 is electrically connected to the timing module 13 through the control main board 2. The photodiode 123 can convert the optical signal into an electrical signal and feedback it to the control main board 2.

[0024] A semiconductor refrigerator 14 is fixedly inserted into the side wall of the circular cavity 7 away from the piston 81, and the refrigerating end of the semiconductor refrigerator 14 is arranged inside the circular cavity 7. The semiconductor refrigerator 14 is electrically connected to the control main board 2. When the semiconductor refrigerator 14 is working, its refrigerating end can transfer the heat inside the circular cavity 7 to the hot end, thereby refrigerating the air inside the circular cavity 7.

[0025] The operating principle of the present invention is described as follows: Collect an appropriate amount of blood sample using a sterile blood collection needle and immediately inject it into a blood collection tube containing an anticoagulant. Gently invert and mix to prevent blood coagulation. Add a diluent to the blood sample according to a certain ratio (the ratio can be set as needed, for example, 1:2), gently shake well to fully dilute, and pour the diluted blood sample into the centrifuge container 1. Install each centrifuge container 1 containing the blood sample on the turntable of the centrifuge equipment in sequence, and connect the cable connectors on each centrifuge container 1 to the interfaces of the control main board 2 (the control main board 2 is installed at the bottom of the turntable of the centrifuge equipment and is connected to the external power supply circuit through a rotary joint. A protective shell and other structures are provided on the outside of the control main board 2 to protect the control main board 2. In order to facilitate the connection of the cable connectors of the centrifuge container 1 to the interfaces of the control main board 2, the interfaces of the control main board 2 can be set on one side of the installation position of each centrifuge container 1). Subsequently, centrifuge the centrifuge container 1 at a speed of 1800 - 2000 revolutions per minute using the centrifuge equipment, and at the same time, start the control main board 2; After the control main board 2 is started, it will control the strip lamp board 51 to work and connect the connection circuit of the photosensitive resistor strip 52 and the electromagnet block 83. The light emitted by the strip lamp board 51 will pass through the centrifuge container 1 and the blood sample inside it and project onto the photosensitive surface of the photosensitive resistor strip 52. Since various substances inside the blood sample are initially mixed together and under the action of centrifugation, various substances start to flow and stratify. For example, the red blood cell layer starts to flow to the lowest side inside the centrifuge container 1. Due to the flow of various substances, the blood sample inside the centrifuge container 1 will continue to flow and change, resulting in continuous change of the light transmitted to the photosensitive resistor strip 52. Therefore, the light intensity at the photosensitive resistor strip 52 will also change continuously. When the light intensity received by the photosensitive resistor strip 52 changes, its own resistance will also change synchronously (within a certain range of light intensity, when the light intensity received by the photosensitive resistor strip 52 increases, its own resistance will decrease synchronously). Therefore, the current intensity supplied to the electromagnet block 83 will also change synchronously (when the resistance of the photosensitive resistor strip 52 decreases, the current intensity supplied to the electromagnet block 83 increases, and vice versa). After the electromagnet block 83 is energized, it will generate a magnetic repulsive force on the permanent magnet 84. When the current intensity supplied to the electromagnet block 83 increases, the magnetic repulsive force generated by the electromagnet block 83 on the permanent magnet 84 increases synchronously. At this time, the displacement distance of the piston 81 increases. When the piston 81 moves away from the electromagnet block 83, it will squeeze the air inside the circular cavity 7. Under the action of the squeezing force and the two one-way valves 93, the air inside the circular cavity 7 is discharged into the arc-shaped air inlet groove 94 through the air outlet hole 92. When the current intensity supplied to the electromagnet block 83 decreases, the magnetic repulsive force generated by the electromagnet block 83 on the permanent magnet 84 decreases. At this time, under the action of the non-magnetic elastic member 82, the piston 81 starts to move back. Since part of the air inside the circular cavity 7 has been discharged, when the piston 81 starts to move back, under the action of the air pressure difference between the inside and outside of the circular cavity 7, the external air will be supplemented into the circular cavity 7 through the air inlet hole 91. Since the current intensity supplied to the electromagnet block 83 changes continuously during the centrifugal separation of the blood sample, the piston 81 will move back and forth, so that gas can be continuously supplied into the arc-shaped air inlet groove 94; The airflow entering the arc-shaped air inlet groove 94 will enter the arc-shaped air outlet groove 95 through two connecting grooves 96 and finally be discharged into the exhaust groove 10 through the exhaust holes 97. After the control main board 2 is started, it will control the operation of each semiconductor cooler 14. The cooling end of the semiconductor cooler 14 can transfer the heat inside the circular cavity 7 to the other end, thereby cooling the air inside the circular cavity 7. When the cold air flows through the arc-shaped air inlet groove 94 and the arc-shaped air outlet groove 95, it can keep the centrifuge container 1 warm and keep the centrifugation temperature in a low temperature state (generally lower than 4°C). Centrifugal separation of white blood cells at a low temperature is conducive to the stable and sufficient separation of white blood cells (at normal temperature, some molecules on the surface of white blood cells may mediate the interaction between cells, leading to cell aggregation. Low temperature can weaken the activity of these molecules and reduce the mutual attraction between cells, thereby preventing white blood cells from aggregating into clumps and facilitating the separation operation); When the airflow is discharged through the exhaust holes 97, under the action of air pressure, one end of the reflective film 111 close to the exhaust holes 97 will bend outward (refer to Figure 8 , Figure 8The dashed part in indicates the bent state of the reflective film 111. When the strip-shaped lamp panel 51 is working, the light rays emitted from one side of it to the light guide column 121 will be conducted by the light guide column 121 to the side wall of the reflective film 111. When there is an air flow discharged through the exhaust hole 97, since one end of the reflective film 111 is blown and bent by the air flow, the position of the reflective film 111 close to the light guide column 121 and the fixed groove 122 is separated from the bottom of the exhaust groove 10. At this time, the light rays irradiated on the side wall of the reflective film 111 will be reflected to the photodiode 123. The photodiode 123 will convert the optical signal into an electrical signal and feedback the electrical signal to the control main board 2. When the control main board 2 receives the electrical signal feedback from the photodiode 123, the control main board 2 will not control the timing module 13 to work. When the reflective film 111 contacts the bottom of the air outlet groove, the light rays irradiated from the light guide column 121 to the reflective film 111 cannot be reflected by the reflective film 111 to the photodiode 123. Therefore, the electrical signal feedback from the photodiode 123 to the control main board 2 disappears. At this time, the control main board 2 will control the timing module 13 to start timing. Since during the centrifugation of the blood sample, the substances in the blood flow, resulting in unstable air flow discharged through the exhaust hole 97, the reflective film 111 will intermittently block the fixed groove 122. Therefore, the photodiode 123 will intermittently feedback electrical signals to the control main board 2. Thus, the timing module 13 cannot continuously time. When the photodiode 123 feedbacks an electrical signal to the control main board 2, the control main board 2 will simultaneously reset the timing information of the timing module 13 and start timing again after the electrical signal feedback from the photodiode 123 disappears. As the centrifugation progresses, the blood sample in the centrifuge container 1 gradually stratifies and tends to be stable. At this time, the intensity of the light rays irradiated on the photosensitive resistor strip 52 also tends to be stable. Therefore, the position of the piston 81 will gradually remain stable. Thus, the air flow discharged from the exhaust hole 97 will gradually disappear. Therefore, one end of the reflective film 111 close to the exhaust hole 97 will not bend. At this time, the photodiode 123 cannot receive light rays. Therefore, the electrical signal it feedbacks to the control main board 2 will be continuously disconnected. Therefore, the timing module 13 will continuously time. When the timing reaches 1 minute, it indicates that the stratification of the blood sample in the centrifuge container 1 has reached sufficient stability. At this time, the timing module 13 will feedback an electrical signal to the main control chip of the control main board 2. The control main board 2 will then send a voice prompt to the staff through the voice module. At this time, the staff can turn off the centrifugation equipment (wherein, the timing module 13 includes multiple independent timing units, and each timing unit can time independently. When processing multiple blood samples, the control main board 2 will only send a voice prompt message after all the timing units feedback electrical signals to the control main board 2); After turning off the centrifugation equipment, separate the cable connectors of each centrifuge container 1 from the control main board 2, gently take out each centrifuge container 1, and then use a pipette to transfer the white blood cell layer in each centrifuge container 1 to a sterile tube to complete the extraction work of white blood cells.

[0026] The above are only the preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be included within the protection scope of the present invention.

Claims

1. A leukocyte extraction device for inhibiting the expression of pro-inflammatory factors, comprising a centrifugal container (1) and a control main board (2) arranged on one side of the centrifugal container (1), characterized in that: Also includes: An outer sleeve (3) is sleeved on the outer side of the centrifugal container (1); the inner wall of the outer sleeve (3) is provided with two mounting grooves (4), and the two mounting grooves (4) are symmetrical about the axis of the centrifugal container (1); the two mounting grooves (4) are jointly provided with a light inspection mechanism (5); A protruding portion (6) is integrally formed and arranged on the upper side of the outer wall of the outer sleeve (3); a circular cavity (7) is provided inside the protruding portion (6); a compression drive mechanism (8) is provided inside the circular cavity (7); and the optical inspection mechanism (5) is electrically connected to the compression drive mechanism (8); A heat preservation unit (9) is arranged inside the outer shell (3), and the compression drive mechanism (8) squeezes the air inside the circular cavity (7) into the heat preservation unit (9); An exhaust groove (10) is provided on the outer wall of the outer jacket (3), and the exhaust groove (10) is connected to the exhaust end of the heat preservation unit (9). A wind shield component (11) and a trigger component (12) are installed inside the exhaust groove (10). A timing module (13) is provided on the control main board (2), and the trigger component (12) is electrically connected to the timing module (13) via the control main board (2).

2. A leukocyte extraction device for inhibiting the expression of pro-inflammatory factors according to claim 1, characterized in that: The light detection mechanism (5) comprises a strip light board (51) and a photoresistor strip (52); the strip light board (51) and the photoresistor strip (52) are both installed in the installation groove (4) on the same side; the light emitted by the strip light board (51) passes through the centrifugal container (1) and is projected onto the photosensitive surface of the photoresistor strip (52); and the strip light board (51) is electrically connected to the control main board (2).

3. A leukocyte extraction device for inhibiting the expression of pro-inflammatory factors according to claim 2, characterized in that: The compression drive mechanism (8) comprises a piston (81) slidably arranged inside the circular cavity (7); a non-magnetic elastic member (82) is fixedly arranged between the side wall of the piston (81) and the circular cavity (7); an electromagnetic block (83) is installed at a position of the cavity wall of the circular cavity (7) located inside the non-magnetic elastic member (82); a permanent magnet (84) is fixedly installed on the side wall of the piston (81) close to the electromagnetic block (83); and the electromagnetic block (83) is electrically connected to the control mainboard (2) via a photoresistor strip (52).

4. A leukocyte extraction device for inhibiting the expression of pro-inflammatory factors according to claim 3, characterized in that: The heat preservation unit (9) comprises an air inlet hole (91) and an air outlet hole (92) formed on the wall of the circular cavity (7); a one-way valve (93) is installed inside the air inlet hole (91) and the air outlet hole (92); an arc-shaped air inlet groove (94) and an arc-shaped air outlet groove (95) are formed on the inner wall of the outer casing (3); the arc-shaped air inlet groove (94) and the arc-shaped air outlet groove (95) are symmetrically arranged on both sides of the centrifugal container (1); two connecting grooves (96) are formed on the lower groove walls of the arc-shaped air inlet groove (94) and the arc-shaped air outlet groove (95); the air outlet hole (92) is connected to the arc-shaped air inlet groove (94); the groove wall of the arc-shaped air outlet groove (95) is provided with an exhaust hole (97); and the exhaust hole (97) is connected to the exhaust groove (10).

5. A leukocyte extraction device for inhibiting the expression of pro-inflammatory factors according to claim 4, characterized in that: The windshield assembly (11) comprises a reflective film (111) movably arranged inside the exhaust groove (10); a pressure strip (112) is fixedly mounted on one side wall of the reflective film (111); the pressure strip (112) is fixedly connected to the bottom of the exhaust groove (10); the side wall of the reflective film (111) is in contact with the bottom of the exhaust groove (10); and the airflow discharged from the exhaust hole (97) is blown to the side wall of the reflective film (111).

6. A leukocyte extraction device for inhibiting the expression of pro-inflammatory factors according to claim 5, characterized in that: The trigger assembly (12) comprises a light guide column (121) arranged on one side of the strip light board (51), one end of the light guide column (121) being arranged in a mounting groove (4) on the same side, and the other end of the light guide column (121) passing through the bottom of the exhaust groove (10), a fixing groove (122) being provided at the bottom of the exhaust groove (10), and a photodiode (123) being installed inside the fixing groove (122), the light emitted by the strip light board (51) being irradiated to the side wall of the reflective film (111) through the conduction of the light guide column (121), and the photodiode (123) being electrically connected to the timing module (13) via the control mainboard (2).

7. A leukocyte extraction device for inhibiting the expression of pro-inflammatory factors according to claim 3, characterized in that: A semiconductor refrigerator (14) is fixedly plugged into a side wall of the circular cavity (7) away from the piston (81), and a cooling end of the semiconductor refrigerator (14) is arranged inside the circular cavity (7). The semiconductor refrigerator (14) is electrically connected to the control mainboard (2).

8. An extraction method applied to the leukocyte extraction device for inhibiting the expression of pro-inflammatory factors as claimed in claim 6, characterized in that: The extraction method comprises the following steps: S1. Use a sterile blood collection needle to collect an appropriate amount of blood sample and immediately inject it into a blood collection tube containing an anticoagulant. Gently invert and mix to prevent blood clotting. S2. Add a diluent to the blood sample in a certain proportion, shake gently to fully dilute, and pour the diluted blood sample into a centrifuge container (1); S3, sequentially installing each centrifugal container (1) containing a blood sample on a turntable of a centrifugal device, and connecting a cable connector on each centrifugal container (1) to an interface of a control mainboard (2); S4, centrifuging the centrifugal container (1) at a speed of 1800-2000 revolutions per minute by a centrifugal device, and simultaneously starting the control main board (2). After the control main board (2) is started, it controls the light detection mechanism (5), the compression drive mechanism (8), the trigger component (12) and the timing module (13) to work; S5. After the control mainboard (2) receives the electrical signal fed back by the timing module (13), the control mainboard (2) sends a prompt voice message to the staff, who can then shut down the centrifugal device; S6. Separate the cable connector of each centrifugal container (1) from the control main board (2), gently take out each centrifugal container (1), and then use a pipette to transfer the white blood cell layer in each centrifugal container (1) into a sterile tube.

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