Method for removing algae in urban water body based on water body bottom mud microorganisms

Abstract

The invention discloses a method for removing algae from an urban water body based on water body bottom mud microorganisms, and the method specifically comprises the following steps: S1, obtaining water bottom mud in a water body to be subjected to algae removal, and refrigerating the water bottom mud; s2, obtaining an algae sample in the water body to be subjected to algae removal, and pretreating the algae sample; s3, preparing a culture medium, and preparing an algae removal culture solution according to the prepared culture medium; s4, performing multiple dilution on the algae removal culture solution according to the water area of the water body to be subjected to algae removal to obtain a diluted algae removal culture solution, and uniformly spraying the diluted algae removal culture solution into the water body to be subjected to algae removal to complete algae removal of the water body. According to the method, water body sediment microbial resources are used as a resource library, microbial populations meeting the requirement for efficient green alga removal of the water body are screened, local materials are used, operation is easy, and the water body eutrophication phenomenon of the urban park pond is conveniently and rapidly solved.

Description

Technical Field

[0001] The present invention belongs to the technical field of water pollution control, and particularly relates to a method for removing algae from urban water bodies based on sediment microorganisms in water bodies. Background Art

[0002] Urban ecological construction is an important guarantee for urban development, and urban garden construction is an important part of urban ecological construction. In urban garden construction, artificial wetlands are important carriers of urban garden landscapes and ecological functions. Urban artificial wetlands generally consist of small rivers, ponds or streams, and are generally static water areas. Due to the daily use of vehicles such as cars and ships, water bodies, soil and air are all polluted to varying degrees, especially by heavy metals and organic pollutants. These pollutants directly enter artificial wetlands in large quantities. At the same time, excessive fertilizers applied during the landscaping maintenance process also accelerate the reproduction of water body algae. The combined effect of these factors has caused the water quality of artificial wetland water bodies in parks to deteriorate slowly, seriously affecting the water body landscape and the exertion of its ecological functions.

[0003] In the prior art, the content of green algae in park water bodies or aquaculture water bodies is too high, and the water bodies show obvious dark green or oily green. Although park water bodies are often manually fished to remove a large amount of filamentous algae, large algae, etc., a large number of tiny single-celled or multi-celled green algae still remain in the water bodies, affecting the water quality of park water bodies. Summary of the Invention

[0004] The purpose of the present invention is to overcome the deficiencies existing in the above prior art, and provide a method for removing algae from urban water bodies based on sediment microorganisms in water bodies. The method of the present invention uses the sediment microorganism resources in the water body as a resource library to screen out microbial populations that are efficient in removing green algae from their own water bodies, making use of local materials, with simple operation, and conveniently and quickly solving the phenomenon of eutrophication of pond water bodies in urban parks.

[0005] In order to achieve the above invention purpose, the technical solution provided by the present invention patent is as follows:

[0006] A method for removing algae from urban water bodies based on sediment microorganisms in water bodies, the method specifically includes the following steps:

[0007] S1, Obtain the bottom sediment in the water body to be de-algaled, and refrigerate the bottom sediment.

[0008] S2, Obtain an algae sample in the water body to be de-algaled, and preprocess the algae sample.

[0009] S3, Prepare a culture medium, and prepare an algae-removing culture solution according to the prepared culture medium.

[0010] S4. Dilute the algae-removing culture solution in multiples according to the water area of the water body to be de-algalized, obtain the diluted algae-removing culture solution, and evenly spray the diluted algae-removing culture solution into the water body to be de-algalized to complete water body de-algalization.

[0011] Further, the collected bottom mud is placed in a sampling bag, and the temperature during refrigeration is 0-5°C.

[0012] Further, the acquisition of the algae sample is specifically as follows: Take 100 L of the water body to be de-algalized as the sample water body. The sample water body contains water body algae. Filter the sample water body by suction to obtain all the algae in the sample water body, which is the algae sample.

[0013] Further, the pretreatment of the algae sample is specifically as follows: Uniformly distribute the obtained algae sample on a filter paper with a diameter of 9 cm, and then divide the filter paper into four equal parts to obtain multiple algae samples.

[0014] Further, the preparation of the culture medium is specifically as follows:

[0015] S31. Extract water from the water body to be de-algalized and divide it into multiple sample water bodies, and add the refrigerated bottom mud to each sample water body;

[0016] S32. After adding the bottom mud to the sample water body, add the algae sample. After adding the algae sample, shake the sample water body at room temperature to culture the culture medium.

[0017] Further, the volume of the sample water body is 100 ml, and the mass of the bottom mud added to the sample water body is 5-10 g.

[0018] Further, before adding the algae sample to the sample water body, cut the filter paper into pieces and then add it to the sample water body.

[0019] Further, the culture of the culture medium is specifically as follows: After the culture medium is prepared, wait until the culture medium becomes turbid and the green color of the culture medium fades or disappears, and then transfer the sample water body in the culture medium to a fresh culture medium for culturing to obtain the culture solution.

[0020] Based on the above technical solutions, the following technical advantages have been achieved through practical application of the method for removing algae from urban water bodies based on water body bottom mud microorganisms in the present invention:

[0021] 1. The method for removing algae from urban water bodies based on water body bottom mud microorganisms in the present invention uses the water body bottom mud microbial resources as a resource library to screen microbial populations that are efficient in removing green algae from their own water bodies. It uses local materials, is simple to operate, and can quickly and conveniently solve the problem of eutrophication of urban park pond water bodies. Specific Embodiments

[0022] To make the objectives, technical solutions, and advantages of the present invention clearer and more explicit, the present invention will be described below through specific examples. However, it should be understood that these descriptions are merely exemplary and are not intended to limit the scope of the present invention. In addition, in the following description, the descriptions of well-known structures and technologies are omitted to avoid unnecessarily confusing the concepts of the present invention.

[0023] The present invention belongs to a method for removing algae from urban water bodies based on sediment microorganisms in water bodies, and the method specifically includes the following steps:

[0024] S1. Obtain the bottom sediment in the water body to be de-algalized, and refrigerate the bottom sediment.

[0025] S2. Obtain an algae sample in the water body to be de-algalized, and preprocess the algae sample.

[0026] S3. Configure a culture medium, and prepare an algae-removing culture solution according to the configured culture medium.

[0027] S4. Dilute the algae-removing culture solution in multiples according to the water area of the water body to be de-algalized to obtain a diluted algae-removing culture solution, and evenly spray the diluted algae-removing culture solution onto the water body to be de-algalized to complete algae removal from the water body.

[0028] The collected bottom sediment is placed in a sampling bag, and the temperature during refrigeration is 0-5°C.

[0029] The specific method for obtaining the algae sample is as follows: Take 100 L of the water body to be de-algalized as a sample water body. The sample water body contains water algae. Filter the sample water body by suction to obtain all the algae in the sample water body, which is the algae sample.

[0030] The specific preprocessing of the algae sample is as follows: Evenly distribute the obtained algae sample on a filter paper with a diameter of 9 cm, and then divide the filter paper into four equal parts to obtain multiple algae samples.

[0031] The specific configuration of the culture medium is as follows:

[0032] S31. Extract water from the water body to be de-algalized and divide it into multiple sample water bodies, and add the refrigerated bottom sediment to each sample water body.

[0033] S32. After adding the bottom sediment to the sample water body, add the algae sample. After adding the algae sample, shake the sample water body at room temperature to culture the culture medium.

[0034] The volume of the sample water body is 100 ml, and the mass of the bottom sediment added to the sample water body is 5-10 g.

[0035] Before adding the algae sample to the sample water body, cut the filter paper into pieces and then add it to the sample water body.

[0036] The cultivation of the culture medium is specifically as follows: after the culture medium is prepared, wait until the culture medium becomes turbid and the green color of the culture medium fades or disappears, and then transfer the sample water body in the culture medium to a fresh culture medium for cultivation to obtain a culture solution.

[0037] The present invention utilizes the rich microbial resources in natural sediment to develop green algae degrading bacteria, which not only enriches the types of green algae degrading bacteria in the market, but also effectively inhibits the overgrowth of algae in the static water bodies of urban park ponds.

[0038] In this patent application, the dilution factor of the algae-removing culture solution is not determined solely based on the water area; other factors include but are not limited to the pollution degree of the water body to be de-algae, the types and densities of algae, water temperature, light conditions, and the expected algae-removing effect, etc., to determine the dilution factor of the algae-removing culture solution;

[0039] Specifically, by setting a basic dilution factor (such as 1:100), and then making appropriate adjustments according to the size of the water area and the above other factors; for example, for a water area with a smaller area, select the value of the basic dilution factor for spraying or spray close to the basic dilution factor; while for a large-area and severely polluted water area, the dilution factor will be reduced (i.e., increase the concentration of the algae-removing culture solution) to improve the algae-removing efficiency.

[0040] Example

[0041] Taking the pond in a certain research institute park as an example;

[0042] 1. Use a grab bucket to grab several 1 kg of the bottom sediment of the algae-containing pond in a certain research institute park, put it into a sampling bag, and refrigerate it at 0 - 5 °C in the refrigerator;

[0043] 2. Take 100 L of the algae-containing water body of the pond in step 1, filter it with a filter paper with a diameter of 9 cm. The green algae are evenly distributed on the filter paper, and the filter paper is cut into 4 equal parts;

[0044] 3. Prepare a microbial culture medium: The culture medium includes the following components:

[0045] 5 g of the bottom sediment of the pond in step 1;

[0046] 1 equal part of the filter paper in step 2 and after cutting the filter paper into pieces;

[0047] 100 ml of the algae-containing water body of the pond in step 1;

[0048] Mix the taken algae-containing water body, bottom sediment, and the cut filter paper; cultivate with constant temperature oscillation; wait until the culture medium becomes turbid and the green color fades or disappears, and transfer it to a fresh culture medium for cultivation;

[0049] In order to expand the cultivation amount, the number of culture bottles can be increased according to actual needs. Collect the culture solution for use and pay attention to preserving the species. The culture solution contains a large number of microbial populations that decompose green algae;

[0050] 4. Dilute the culture solution obtained in step 3 by 100 - 500 times and evenly spray it into the pond water body, and regularly track and monitor the water quality of the pond water body.

[0051] The small - scale wetland in the park of a certain research institute consists of three small ponds, namely the south - side, east - side and north - side ponds, which are interconnected. The experiment was carried out in the south - side and north - side ponds. The algicidal bactericide was prepared according to steps 1 - 4. The obtained algicidal bacterium solution was diluted 100 times and sprayed into the south - side and north - side ponds respectively. The middle east - side pond was used as a control, and the results were observed after 30 days.

[0052]

[0053] As can be seen from the above table, the CO and chlorophyll contents in the south - side pond sprayed with the algicidal bacterium solution and the measured pond are significantly lower than those in the east - side pond without spraying the algicidal bacterium solution, indicating that the algicidal method of the present invention has the function of removing algae; the present invention develops green - algae - degrading bacteria by using the rich microbial resources in natural bottom mud, which not only enriches the types of green - algae - degrading bacteria in the market, but also effectively inhibits the over - proliferation of algae in the static water bodies of urban park ponds. By culturing the corresponding bacterium solution for urban park ponds in different regions, the pertinence of algae removal is improved.

[0054] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and not to limit them; although the present invention has been described in detail with reference to the preferred embodiments, those of ordinary skill in the art should understand that it is still possible to modify the specific implementation manners of the invention or make equivalent replacements for some technical features; without departing from the spirit of the technical solutions of the present invention, they should all be covered within the scope of the technical solutions claimed by the present invention.

Claims

1. A method for removing algae from urban water bodies based on water sediment microorganisms, characterized in that: The method specifically comprises the following steps: S1, obtaining bottom mud from a water body to be de-algaeed, and refrigerating the bottom mud; S2, obtaining an algae sample from a water body to be de-algaeed, and pre-treating the algae sample; S3, preparing a culture medium, and preparing an algae removal culture solution according to the prepared culture medium; S4, diluting the algae removal culture solution multiple times according to the area of ​​the water body to be algae removed, obtaining the diluted algae removal culture solution, and spraying the diluted algae removal culture solution evenly into the water body to be algae removed, thereby completing the algae removal of the water body.

2. The method for removing algae from urban water bodies based on water sediment microorganisms according to claim 1, characterized in that: The collected bottom mud is placed in a sampling bag and refrigerated at a temperature of 0-5°C.

3. The method for removing algae from urban water bodies based on water sediment microorganisms according to claim 1, characterized in that: The algae sample is obtained specifically as follows: 100L of water to be de-algaeed is taken as a sample water body, the sample water body contains water algae, the sample water body is filtered, and all the algae in the sample water body are obtained as the algae sample.

4. The method for removing algae from urban water bodies based on water sediment microorganisms according to claim 3, characterized in that: The algae sample pretreatment is specifically as follows: the obtained algae sample is evenly distributed on a filter paper with a diameter of 9 cm, and then the filter paper is divided into four equal parts to obtain a plurality of algae samples.

5. The method for removing algae from urban water bodies based on water sediment microorganisms according to claim 1, characterized in that: The culture medium configuration is specifically as follows: S31, extracting water from the water body to be de-algaeed and dividing it into a plurality of sample water bodies, and adding refrigerated bottom mud to each sample water body; S32, adding the bottom mud to the sample water body and then adding the algae sample, after adding the algae sample, shaking the sample water body at room temperature to cultivate the culture medium.

6. The method for removing algae from urban water bodies based on water sediment microorganisms according to claim 5, characterized in that: The volume of the sample water body is 100 ml, and the mass of the bottom mud added to the sample water body is 5-10 g.

7. The method for removing algae from urban water bodies based on water sediment microorganisms according to claim 5, characterized in that: Before adding the algae sample into the sample water body, the filter paper is cut into pieces and then added into the sample water body.

8. The method for removing algae from urban water bodies based on water sediment microorganisms according to claim 5, characterized in that: The culture medium is specifically cultured as follows: after the culture medium is configured, wait until the culture medium becomes turbid and the green color of the culture medium becomes lighter or disappears, and then transfer the sample water in the culture medium to a fresh culture medium for cultivation to obtain a culture solution.

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