Small animal isolated organ perfusion platform and use method

Abstract

The novel small animal isolated organ perfusion platform and the using method thereof are specially designed for in-vitro research and preservation of small animal organs, and the technical problem that existing large animal perfusion equipment is difficult to adapt to the small animal organs is solved. The platform adopts a modular design and comprises a miniaturized closed organ container, a heat exchange system, a perfusion system, a membrane lung gas exchange module and a real-time monitoring and control system. The organ container consists of a transparent cover body and a cylindrical base, and is provided with a standardized pagoda-shaped interface and a special arteriovenous and trachea cannula, so that stable connection and dynamic regulation and control of organs are supported, and tissue damage is prevented; the perfusion system adopts a high-precision rolling pump to provide power, and supports accurate adjustment of flow and pressure; the membrane lung module is connected with an external gas supply device, the oxygen concentration of perfusate can be dynamically adjusted, and the gas environment of pulmonary arteries and veins is simulated.

Description

Technical Field

[0001] The present invention relates to a perfusion platform, and more specifically to a perfusion platform for small animal ex vivo organs and its usage method. Background Art

[0002] Ex vivo organ perfusion technology is of great significance in life science and medical research. By simulating the in vivo environment to supply and maintain the functions of ex vivo organs, this technology is widely used in fields such as organ transplantation, drug screening, pathophysiological research, and organ function evaluation. Currently, typical organ preservation methods mainly rely on hypothermic preservation, that is, placing the organs on ice or in chemical preservation solutions. For example, for the preservation of the lungs, the inflated lungs are usually collected under sterile conditions, and the blood and impurities are removed by rinsing. Subsequently, the lungs are perfused with a hypothermic preservation solution, and the perfused lungs are placed in a sterile bag or a special preservation box, and then stored in a 4°C refrigerator or a constant temperature refrigeration device. However, in the state of hypothermic cold preservation, it is difficult to achieve the exchange of organ perfusion fluid and gas, which easily leads to the accumulation of metabolites and gases. Taking the lungs as an example, after the cold preservation time exceeds 4 hours, its function may decline significantly, and even fail to meet the requirements of subsequent transplantation. In addition, during the rewarming process of the lungs after hypothermic preservation, the temperature change is likely to cause tissue damage, further reducing its functional activity. In contrast, ex vivo organ perfusion technology can simulate the in vivo physiological environment, not only significantly extending the organ preservation time, but also effectively improving the organ preservation quality.

[0003] Currently, most of the existing ex vivo organ perfusion devices are designed for large animal organs and are mainly used for the research of ex vivo organs of large-sized animals such as pigs. Due to the small size and flow requirements of small animal organs and their complex physiological characteristics, it is difficult to be compatible with the existing systems. Currently, there is a lack of a systematic perfusion platform for small animal ex vivo organs to provide a stable and controllable external environment to support the stable development of experiments. Traditional perfusion platforms usually require separate design and serial connection of various functional modules and instruments, resulting in complex operations, long experimental cycles, and high costs. To address these problems, the present invention proposes a novel perfusion platform for small animal ex vivo organs and its usage method, aiming to fill the relevant technical gaps and promote the research and application development in the field of organ preservation technology. Summary of the Invention

[0004] Aiming at the deficiencies existing in the prior art, the purpose of the present invention is to provide a perfusion platform for small animal ex vivo organs and its usage method, which is mainly applicable to the perfusion of small animal ex vivo organs.

[0005] To achieve the above purpose, the present invention provides the following technical solutions: A perfusion platform for small animal ex vivo organs, comprising: An organ container, which is installed on an organ container support and is used for placing small animal ex vivo lungs; A liquid storage tank, which is used to store the organ perfusion fluid in the circulation, is connected to the organ container through a roller pump and is used to output the organ perfusion fluid into the organ container; A heat exchange water tank, which is connected to the liquid storage tank to maintain the temperature of the organ perfusion fluid in the liquid storage tank; A membrane lung is connected to the isolated lung of a small animal in the organ container through a pulmonary artery circulation pipeline, so that the hypoxic perfusion fluid enters the isolated lung of the small animal through the pulmonary artery pipeline; A ventilator is connected to the isolated lung of a small animal in the organ container through a tracheal intubation and is used to provide respiratory support for the isolated lung; Among them, the organ container is composed of a container base and a transparent cover body. The transparent cover body is hermetically covered on the opening at the upper end of the container base. There are three pipeline interfaces on the transparent cover body. The upper ends of the three pipeline interfaces are respectively connected to the pulmonary artery circulation pipeline, the pulmonary vein circulation pipeline and the ventilator, and the lower ends are respectively connected with an arterial intubation, a venous intubation and a tracheal intubation to be used for connecting the arterial interface, the venous interface and the tracheal interface of the isolated lung of a small animal. A clamping head is fixed at one end of the arterial intubation, the venous intubation and the tracheal intubation facing away from the transparent cover body, so as to clamp the ends of the arterial intubation, the venous intubation and the tracheal intubation with the arterial interface, the venous interface and the tracheal interface of the isolated lung of a small animal when connecting them.

[0006] As a further improvement of the present invention, the clamping head of the arterial intubation includes a bottom ring and a plug. The bottom ring is in the shape of a hollow cylinder. One end is sleeved on the end of the arterial intubation, and the other end is fixed to the plug. A first clamping step is formed between the end of the bottom ring and the outer wall of the arterial intubation.

[0007] As a further improvement of the present invention, the plug includes a connecting ring and a tapered head. The tapered head is a hollow structure. The bottom surface of the tapered head is coaxial with the connecting ring. The connecting ring is coaxially fixed to the end of the bottom ring facing away from the arterial intubation, so as to form a second clamping step between the end of the connecting ring and the outer side wall of the bottom ring.

[0008] As a further improvement of the present invention, the clamping head of the tracheal intubation includes an insertion tube and a connecting head. The insertion tube is connected to the tracheal intubation through the connecting head. A plurality of clamping grooves are formed on the outer side wall of the end of the insertion tube facing away from the connecting head.

[0009] On the other hand, the present invention provides a usage method of the above perfusion platform, including the following steps: Step 1: After injecting and anesthetizing the experimental animal, place it face up on the test bench, firmly fix the four limbs, shave and disinfect the chest, open the chest to expose the lungs, separate the fascia and free tissues, and respectively connect the pulmonary artery blood vessel, the left heart sleeve, and the trachea to the arterial intubation, the venous intubation, and the tracheal intubation; Step 2: Transfer the excised small animal lung into an organ container and fix it by connecting it to the corresponding pipelines through arterial intubation, tracheal intubation, and venous intubation; Step 3: Start the roller pump to draw the perfusion fluid from the liquid storage tank and deliver the perfusion fluid with a set temperature and flow rate to the organ container; Step 4: Adjust the membrane lung and the ventilator, and implement gas exchange and oxygen concentration control through the arterial circulation circuit and the venous circulation circuit to maintain an internal environment approximately in a physiological state for the organ; Step 5: Use monitoring components such as the pressure sensors on the arterial and venous sides, the blood oxygen sensors, and the three-way sampling ports to obtain the perfusion fluid parameters in real time and adjust the perfusion conditions in a timely manner according to the experimental requirements.

[0010] For a further improvement of the above usage method, the method of transferring the excised small animal lung into the organ container in Step 2 is as follows: First, place a transparent sterile U-shaped cover under the excised small animal lung, then place the excised small animal lung on the transparent sterile U-shaped cover, then put the transparent sterile U-shaped cover into the organ container, and then operate the transparent cover of the organ container to cover the opening of the container base, and realize the clamping and fixing of the transparent sterile U-shaped cover through the cooperation between the transparent cover and the container base to support the small animal lung.

[0011] Advantages of the present invention: 1. The small animal excised organ perfusion platform provided by the present invention overcomes the technical bottleneck that existing equipment is difficult to adapt to the organ size and physiological characteristics of small animals through optimized design. By using the design of the clamping head, after the intubation is completed, the arterial intubation, venous intubation, and tracheal intubation can be clamped by the clamping head, which is convenient for even experimental personnel with poor catheter placement skills to complete the intubation operation. At the same time, the intubation operation causes no damage to the organ and blood vessels, and can provide a stable and controllable external environment for the experiment, realizing precise regulation of the perfusion fluid flow rate, pressure, and temperature, thereby significantly extending the organ preservation time and improving the preservation quality. At the same time, the platform supports perfusion fluid circulation and gas exchange, effectively reducing the damage to organ function caused by the accumulation of metabolites and gases.

[0012] 2. The platform adopts a modular and integrated design, simplifies and standardizes the experimental operation process, significantly improves the scientific research experiment efficiency, and reduces the equipment operation cost at the same time. This technology provides reliable support for the preservation, function research of small animal excised organs, and related disease model experiments, fills the technical gap in the field of small animal lung perfusion in China, and shows broad application prospects. Description of the drawings

[0013] Figure 1 It is a module schematic diagram of the small animal excised organ perfusion platform of the present invention; Figure 2 is Figure 1Schematic diagram of the structure of the organ container; Figure 3 is Figure 1 Schematic diagram of the structure of the arterial cannula in Figure 4 is Figure 1 Schematic diagram of the structure of the tracheal cannula in Detailed implementation manner

[0014] The present invention will be further described in detail below with reference to the embodiments given in the drawings.

[0015] Referring to Figures 1 to 4 As shown, a small animal ex vivo organ perfusion platform of this embodiment includes: An organ container 2, which is installed on an organ container bracket 1 and is used for placing a small animal ex vivo lung; A liquid storage tank 4, which is used for storing the organ perfusion liquid in the circulation, is communicated with the organ container 2 through a roller pump 5, and is used for outputting the organ perfusion liquid into the organ container 2; A heat exchange water tank 3, which is communicated with the liquid storage tank 4 to maintain the temperature of the organ perfusion liquid in the liquid storage tank 4; A membrane lung 6, which is connected to the small animal ex vivo lung in the organ container 2 through a pulmonary artery circulation pipeline 20, so that the hypoxic perfusion liquid enters the small animal ex vivo lung through the pulmonary artery pipeline; A ventilator 8, which is connected to the small animal ex vivo lung in the organ container 2 through a tracheal cannula and is used for providing respiratory support to the ex vivo lung; Wherein, the organ container 2 is composed of a container base and a transparent cover body. The transparent cover body is hermetically covered on the opening at the upper end of the container base. There are 3 pipeline interfaces on the transparent cover body. The upper ends of the 3 pipeline interfaces are respectively connected to the pulmonary artery circulation pipeline 20, the pulmonary vein circulation pipeline 21 and the ventilator 8, and the lower ends are respectively connected with an arterial cannula 9, a venous cannula 11 and a tracheal cannula 10 for connecting the arterial interface, venous interface and tracheal interface of the small animal ex vivo lung. The ends of the arterial cannula 9, venous cannula 11 and tracheal cannula 10 facing away from the transparent cover body are all fixed with clamping heads, so that when connecting the arterial interface, venous interface and tracheal interface of the small animal ex vivo lung, the ends of the arterial cannula 9, venous cannula 11 and tracheal cannula 10 can be clamped with the arterial interface, venous interface and tracheal interface of the small animal ex vivo lung. During the process of cannulating the small animal lung, in principle, one cannulation is performed. If the operation is improper once, it is very easy to damage the small animal lung. For this reason, in this embodiment, the method of fixing clamping heads at the ends of the arterial cannula 9, venous cannula 11 and tracheal cannula 10 facing away from the transparent cover body is adopted, so that the function of the clamping head can be utilized to realize direct fixation after one cannulation, and avoid the situation that the ends of the arterial cannula 9, venous cannula 11 and tracheal cannula 10 need to be re-cannulated due to improper cannulation operation, which may damage the small animal lung.

[0016] Further, in this embodiment, the first clamping step is adopted to achieve one-time clamping. Specifically, the elastic parts of the arterial interface, venous interface, and tracheal interface are used to wrap the first clamping step to achieve clamping. The first clamping step is composed of a bottom ring 91 and a plug 92. The bottom ring 91 is a hollow cylinder. One end is sleeved on the end of the arterial cannula 9, and the other end is fixed to the plug 92. The end of the bottom ring 91 and the outer wall of the arterial cannula 9 form the first clamping step.

[0017] Further, in order to further enhance the clamping effect in this embodiment, a second clamping step is also provided. The second clamping step is realized by the plug 92, specifically a connecting ring 921 and a tapered head 922. The tapered head 922 is a hollow structure. The bottom surface of the tapered head 922 is coaxial with the connecting ring 921. The connecting ring 921 is coaxially fixed to the end of the bottom ring 91 facing away from the arterial cannula 9, so that a second clamping step is formed by the end of the connecting ring 921 and the outer side wall of the bottom ring 91.

[0018] Further, in this embodiment, by providing triangular holes for liquid circulation on the side surface of the tapered head 922 and making the tapered part of the tapered head a rounded corner structure, the liquid passing amount of the tapered head can be maximally guaranteed, and at the same time, the situation of damage caused by the overly sharp head of the tapered head during the intubation process can be avoided.

[0019] Further, based on the differences between the tracheal interface and the arterial interface and venous interface in this embodiment, different connector head structures are provided, specifically an insertion tube 101 and a connector 102. The insertion tube 101 is connected to the tracheal cannula 10 through the connector 102. A plurality of clamping grooves 103 are provided on the outer side wall of the end of the insertion tube 101 facing away from the connector 102.

[0020] The structure of the venous cannula 11 in this embodiment is the same as that of the arterial cannula 9, so it will not be elaborated here.

[0021] This embodiment also provides a usage method, which specifically includes the following steps: Step 1: After injecting anesthetic into the experimental animal, place it on the test bench on its back, firmly fix the four limbs, shave and disinfect the chest, open the chest to expose the lungs, separate the fascia and free tissues, and connect the pulmonary artery blood vessel, left heart cuff, and trachea to the arterial cannula 9, venous cannula 11, and tracheal cannula 10 respectively; Step 2: Transfer the isolated small animal lung to the organ container 2, and connect and fix it to the corresponding pipelines through the arterial cannula 9, tracheal cannula 1, and venous cannula 11; Step 3: Start the roller pump 5 to draw the perfusion fluid from the liquid storage tank 3 and transport the perfusion fluid with a set temperature and flow rate to the organ container 2; Step 4: Adjust the membrane lung 6 and the ventilator 8, and implement gas exchange and oxygen concentration control through the arterial circulation circuit 20 and the venous circulation circuit 21 to maintain an internal environment with an approximate physiological state for the organ. Step 5: Use monitoring components such as pressure sensors, blood oxygen sensors on the arterial and venous sides, and three-way sampling ports to obtain perfusion fluid parameters in real time and adjust the perfusion conditions in a timely manner according to experimental requirements.

[0022] In the above method, to facilitate the user's operation of intubation and the operation of the isolated small animal lung, in this embodiment, the method of transferring the isolated small animal lung into the organ container 2 is as follows: First, a transparent sterile U-shaped cover is placed under the isolated small animal lung, and then the isolated small animal lung is placed on the transparent sterile U-shaped cover. After that, the transparent sterile U-shaped cover is placed into the organ container 2. Then, the transparent cover of the organ container 2 is operated to cover the opening of the container base, and the transparent sterile U-shaped cover is clamped and fixed through the cooperation between the transparent cover and the container base to support the small animal lung. At the same time, a magnifying glass can be used to assist intubation during the intubation process. Or in this embodiment, the middle part of the transparent cover is processed into a convex lens structure, and the three pipeline interfaces are evenly distributed on the edge of the convex lens structure in the transparent cover. A handle is provided on the side of the transparent cover. During the intubation of the small animal lung, the transparent cover can be removed by loosening the screws, and then the transparent cover can be used as a magnifying glass by holding the handle to facilitate intubation. On the other hand, when the transparent cover is installed on the container base, the convex lens structure can facilitate people to better observe the operation status of the small animal lung.

[0023] Based on the above perfusion platform and usage method, the following examples are provided in this embodiment: 1. Weigh, anesthetize, and fix the rat experimental animal to the experimental table. 2. Disinfect the chest of the rat, open the chest, separate the subcutaneous tissue under the microscope, free the muscle tissue, open the pericardium and mediastinum, cut open the sternum and expose the rat lungs. 3. In the in-vivo state of the lungs, cut open and expose the trachea, insert one end of the tracheal intubation and fix it to the rat trachea, and connect the other end to the ventilator. Set the tidal volume to 4 ml / kg and the positive end-expiratory pressure to 2 cmH2O to maintain the normal state of the lungs. 4. Clamp the superior and inferior vena cava at the rat chest cavity with a tracheal clamp, make a 2-3 mm incision in the pulmonary artery trunk, cut open and drain blood from the left heart sleeve, place the arterial intubation into the incision and the main pulmonary artery, fix it with silk thread, and inject organ perfusion fluid into the pulmonary artery trunk through a syringe to perform antegrade perfusion of the lungs. 5. Connect the pulmonary vein intubation at the left heart sleeve, and inject organ perfusion fluid into the pulmonary vein intubation through a syringe to perform retrograde perfusion of the lungs. 6. Place the isolated rat lungs in an organ container and fix them by connecting them to the corresponding pipelines through arterial cannula 9, tracheal cannula 10, and venous cannula 11. Fix the lungs to the extracorporeal circulation platform and then perform organ perfusion. 1. While obtaining the donor lung in the first step and before connecting the lung to the perfusion circuit, add 500 ml of organ perfusion fluid to the reservoir and maintain the system at 37 °C through a heat exchanger. 2. Use the organ perfusion fluid to remove the residual gas in the lung organ, connect the overall perfusion circuit in series, start the roller pump and slowly increase the flow rate to 8 ml / min. Draw the perfusion fluid from the reservoir and deliver the 37 °C perfusion fluid to the organ container for ex vivo perfusion of the rat lungs. 3. Adjust the membrane lung and the ventilator. The ventilator ventilates with normal ambient air, with a tidal volume of 3 - 6 ml / kg, a respiratory rate of 7 bpm, and a positive end-expiratory pressure of 3 cmH2O. The membrane lung perfuses with a low-oxygen concentration gas of 86% N2; 6% O2; 8% CO2 to deoxygenate the perfusion fluid and maintain an approximately physiological internal environment for the organ. 4. Monitor the experimental environment parameters during the perfusion process through the pressure, blood oxygen sensors, and flow sensors on the arterial and venous sides, which are used for feedback regulation of pump speed settings, ventilator parameter settings, checking whether the circuit is blocked, replenishing organ perfusion fluid and related experimental drugs, etc.

[0024] 5. During the perfusion process, at fixed intervals or according to the preset experimental requirements, draw the organ perfusion fluid from the three-way sampling port for further research and analysis. After the perfusion is completed, remove the rat lungs and, according to the experimental protocol and purpose, perform cold preservation on the lungs or conduct further biochemical and pathological analyses.

[0025] In summary, the organ perfusion platform and the usage method provided in this embodiment 1. Technical adaptability for small animal organs: The present invention is specifically designed for small animal ex vivo organs. Aiming at the technical problem that existing equipment is mostly designed for large animals and is difficult to adapt to small animal organs, through structural optimization and parameter adjustment, a high-precision and miniaturized solution is provided. The platform adopts a compact design, including a miniaturized airtight organ container made of transparent material for easy experimental observation, and the airtight structure can effectively avoid external contamination. The container is equipped with standardized tower-shaped interfaces, as well as specially designed arterial, venous, and tracheal cannulas for connecting arteries, veins, and tracheas, ensuring stable connection of small organs while facilitating experimental operations and reducing organ damage. The container also supports dynamic regulation of pressure and humidity. The support part of the platform is designed to be height-adjustable to adapt to small animal organs of different sizes, keeping them stable and easy to operate during the experiment.

[0026] 2. Strengthen real-time regulation and automation functions: The present invention integrates a multi-parameter sensing and monitoring and automation control system to achieve real-time monitoring and regulation of the experimental process, significantly improving the accuracy and efficiency of the experiment. The platform embeds highly sensitive pressure sensors, blood oxygen sensors, and temperature sensors in the arterial and venous circulation circuits to continuously monitor core parameters such as the pressure, oxygen concentration, and temperature of the perfusion fluid. The data collected by the sensors is transmitted to the control system through the data acquisition module, and the control system can be used to guide the operator to adjust conditions such as the perfusion pump speed and temperature based on real-time feedback to ensure that the perfusion conditions are always maintained in the optimal state. In addition, the platform is equipped with a rapid sampling port that can collect perfusion fluid samples at any time without disturbing the perfusion process for subsequent biochemical and metabolic index analysis. To ensure the stability of the experimental process, the system also has an automatic anomaly detection and alarm function. When the monitored parameters exceed the preset range, the system will automatically issue an alarm prompt to remind for human intervention, improving the safety and reliability of the experimental process.

[0027] 3. Specific implementation methods of functional innovation: Through modular design and function optimization, the present invention provides comprehensive technological innovation, further enhancing the practicality and flexibility of the platform. The organ container module adopts a transparent and sealed design, includes a standardized threaded interface, is compatible with a variety of perfusion pipelines, and is additionally equipped with a humidity adjustment interface. Combined with a micro steam generator, it realizes dynamic control of the humidity inside the container, effectively preventing dry damage to the organ surface. The membrane lung gas exchange module is connected to an external gas supply system and can freely adjust the gas mixing ratio according to experimental needs, dynamically realizing the switching between hypoxic and hyperoxic states of the perfusion fluid. The oxygen concentration range can be accurately switched by switching the gas supply cylinders, thus precisely simulating the physiological environment of the pulmonary artery and vein. The heat exchange and temperature control module maintains a constant temperature of the perfusion fluid through the jacketed glass structure of the liquid storage tank and the hot water circulation system, and cooperates with the temperature sensor for real-time monitoring and regulation. In addition, the platform supports rapid sampling and real-time data analysis. Through the linkage of the sensors and the control module, a comprehensive monitoring and regulation closed-loop is formed, suitable for complex organ preservation and function research scenarios.

[0028] The above are only the preferred embodiments of the present invention, and the protection scope of the present invention is not limited to the above embodiments. All technical solutions falling within the concept of the present invention belong to the protection scope of the present invention. It should be noted that for those of ordinary skill in the art, without departing from the principle of the present invention, several improvements and refinements should also be regarded as within the protection scope of the present invention.

Claims

1. A small animal isolated organ perfusion platform, characterized in that: include: An organ container (2), the organ container (2) being mounted on an organ container support (1) and used for placing an isolated lung of a small animal; A liquid storage tank (4) for storing the organ perfusion liquid in circulation, connected to the organ container (2) via a roller pump (5) for outputting the organ perfusion liquid into the organ container (2); A heat exchange water tank (3) is connected to the liquid storage tank (4) to maintain the temperature of the organ perfusion liquid in the liquid storage tank (4); The membrane lung (6) is connected to the isolated lung of the small animal in the organ container (2) via a pulmonary artery circulation line (20), so that the hypoxic perfusion fluid enters the isolated lung of the small animal via the pulmonary artery line; A ventilator (8) connected to the isolated lung of a small animal in the organ container (2) via an endotracheal tube (10) to provide respiratory support to the isolated lung; The organ container (2) is composed of a container base and a transparent cover body, wherein the transparent cover body is sealed over the opening at the upper end of the container base, and the transparent cover body is provided with three pipeline interfaces, wherein the upper ends of the three pipeline interfaces are respectively connected to the pulmonary artery circulation pipeline (20), the pulmonary vein circulation pipeline (21) and the ventilator (8), and the lower ends are respectively connected to an arterial cannula (9), a venous cannula (11) and a tracheal cannula (10), so as to be used for connecting the arterial interface, the venous interface and the tracheal interface of the isolated lung of the small animal. The ends of the arterial cannula (9), the venous cannula (11) and the tracheal cannula (10) facing away from the transparent cover body are all fixed with a clamping joint part, so that when connecting the arterial interface, the venous interface and the tracheal interface of the isolated lung of the small animal, the ends of the arterial cannula (9), the venous cannula (11) and the tracheal cannula (10) are clamped with the arterial interface, the venous interface and the tracheal interface of the isolated lung of the small animal.

2. The small animal isolated organ perfusion platform according to claim 1, characterized in that: The clamping joint part of the arterial cannula (9) comprises a bottom ring (91) and a plug (92); the bottom ring (91) is in the shape of a hollow cylinder, one end of which is sleeved onto the end of the arterial cannula (9), and the other end of which is fixed to the plug (92); the end of the bottom ring (91) and the outer wall of the arterial cannula (9) form a first clamping step.

3. The small animal isolated organ perfusion platform according to claim 2, characterized in that: The plug (92) comprises a connecting ring (921) and a conical head (922); the conical head (922) is a hollow structure; the bottom surface of the conical head (922) is coaxial with the connecting ring (921); the connecting ring (921) is coaxially fixed to an end of the bottom ring (91) facing away from the arterial cannula (9), so that a second clamping step is formed by the end of the connecting ring (921) and the outer side wall of the bottom ring (91).

4. The small animal isolated organ perfusion platform according to claim 3, characterized in that: A triangular-shaped hole for liquid circulation is provided on the side of the conical head (922), and the conical portion of the conical head is a rounded structure.

5. The small animal isolated organ perfusion platform according to claim 4, characterized in that: The clamping joint of the endotracheal tube (10) comprises an insertion tube (101) and a connector (102); the insertion tube (101) is connected to the endotracheal tube (10) via the connector (102); a plurality of clamping grooves (103) are provided on the outer side wall of the end of the insertion tube (101) facing away from the connector (102).

6. A method for using the small animal isolated organ perfusion platform according to any one of claims 1 to 5, characterized in that: The steps include: Step 1: After injecting anesthesia, the experimental animal is placed on its back on the test bench, and the limbs are firmly fixed. The chest is shaved and disinfected, the chest is opened to expose the lungs, the fascia and free tissues are separated, and the pulmonary artery, left heart cuff, trachea and arterial cannula (9), venous cannula (11), and tracheal cannula (10) are connected respectively; Step 2, transferring the isolated small animal lung into the organ container (2), and connecting and fixing it with corresponding pipelines through the arterial cannula (9), the tracheal cannula (1), and the venous cannula (11); Step 3, starting the roller pump (5) to draw perfusion fluid from the fluid storage tank (3) and delivering the perfusion fluid with a set temperature and flow rate to the organ container (2); Step 4: regulating the membrane lung (6) and the ventilator (8), implementing gas exchange and oxygen concentration control through the arterial circulation circuit (20) and the venous circulation circuit (21), so as to maintain an internal environment close to the physiological state for the organs; Step 5: Use the monitoring components such as pressure and blood oxygen sensors on the arterial and venous sides and the three-way sampling port to obtain the perfusion fluid parameters in real time and adjust the perfusion conditions in time according to the experimental requirements.

7. The method for using the small animal isolated organ perfusion platform according to claim 6, characterized in that: The method for transferring the isolated small animal lungs into the organ container (2) in step 2 is as follows: first, a transparent sterile U-shaped cover is placed under the isolated small animal lungs, and then the isolated small animal lungs are placed on the transparent sterile U-shaped cover, and then the transparent sterile U-shaped cover is placed into the organ container (2), and then the transparent cover of the organ container (2) is operated to cover the opening of the container base, and the transparent sterile U-shaped cover is clamped and fixed by the cooperation between the transparent cover and the container base to support the small animal lungs.

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