Alfalfa field soil sealing herbicide and application thereof

Abstract

The invention relates to the technical field of pesticides, in particular to a clover field soil sealing herbicide and application thereof. The active ingredients of the herbicide comprise acetochlor and trifluralin, or acetochlor and imazethapyr. The invention has the following beneficial effects: through binary compounding of acetochlor and trifluralin or binary compounding of acetochlor and imazethapyr, and in combination with precisely optimized proportioning parameters and field application amount, on the premise of ensuring that the emergence rate of crops and the growth indexes (plant height, root length and biomass accumulation) of seedlings are significantly improved, the yield of the seedlings is increased, and the yield of the seedlings is increased. Similarly, the weed plant control effect can reach 95% or above. By using the herbicide, the safety of crops can be guaranteed to the greatest extent, the optimal weeding effect can be realized, and the herbicide has important popularization and application values.

Description

Technical Field

[0001] The present invention relates to the technical field of pesticides, and in particular to a soil-sealing herbicide for alfalfa fields and its uses. Background Art

[0002] Alfalfa (Medicago sativa) is a perennial leguminous plant. Due to the land grade, climate conditions in alfalfa planting areas in China, especially the influence of weeds, only 5.0% of domestic super-grade and first-grade alfalfa is of high quality, 40.0% is first-grade alfalfa, and 44.0% is second-grade alfalfa. From the perspective of the quality positioning of domestic alfalfa commercial grass, domestic alfalfa still mainly consists of medium- and low-end quality products. Therefore, improving the yield and quality of alfalfa is the main problem to be solved in alfalfa production in China at present. In recent years, with the continuous expansion of alfalfa planting area, weeds have become one of the bottlenecks restricting the development of the alfalfa industry.

[0003] Soil-sealing herbicides are one of the important chemical weeding means in modern agricultural production. In recent years, new soil-sealing herbicides have been continuously developed. These products usually have higher selectivity, a wider weeding spectrum, stronger soil stability, and lower environmental impact. In view of different crop types, soil conditions, and climate characteristics, researchers are optimizing the application technologies of soil-sealing herbicides, including the optimal application period, application dosage, application method, and comprehensive supporting technologies with other weeding measures. With long-term use, the enhanced resistance of weed populations to herbicides is a common problem. In addition, in alfalfa fields, there is less research on the mixture of soil-sealing herbicides, and the mixture application effect is not good. Therefore, scientists are studying how to delay the emergence and development of herbicide resistance through rotation of herbicides, mixture of herbicides, and development of new action mechanisms. Given the increasing attention to the ecological environment and food safety, the development of low-toxic, low-residue, and biodegradable soil-sealing herbicides has become a trend, and at the same time, the exploration of environmentally friendly herbicides derived from biological sources and natural products is also underway.

[0004] Soil-sealing herbicides have a long effective period and can fully improve the competitive advantage of crops. As the first step in controlling weeds, they are widely used in agriculture, but there is no soil-sealing herbicide registered for alfalfa fields in China. In the central region of Inner Mongolia, the main soil-sealing herbicides for alfalfa fields are trifluralin, acetochlor, and pendimethalin. The weeding spectrum of single application of soil-sealing herbicides has limitations, and long-term single application will cause weeds to develop resistance, and then increase the usage amount, resulting in the impact on soil flora and environmental pollution.

[0005] As a commonly used soil - closed herbicide, acetochlor also has some disadvantages in agricultural applications. For example, if acetochlor is applied in excessive amounts or under inappropriate application conditions (such as unsuitable temperature, humidity, and soil type), it is likely to cause phytotoxicity to crops, especially sensitive crops, manifested as inhibited crop growth, chlorosis of leaves, root damage, etc. In severe cases, it can lead to the death of crop seedlings. When acetochlor is used alone for a long time, it is easy to induce the resistance of weed populations, reducing the effectiveness of the herbicide, forcing farmers to increase the dosage or replace it with higher - level pesticides, forming a vicious cycle. As a pre - emergence herbicide, acetochlor requires application within a certain period after crop sowing and before weed germination, with a narrow application window and high requirements for farming operations. Although acetochlor has a certain selectivity for some crops, it may still have negative impacts on some crops under specific conditions, limiting its versatility in fields of various crops.

[0006] The pure product of trifluralin is orange - yellow crystals, with certain volatility and photodegradability. Its water solubility is extremely low, it is easily adsorbed by soil colloids and not easily leached, and it is not easily mobile in the soil. It is easily decomposed in the soil through volatilization and photodegradation, with less residue. It is mainly absorbed through the coleoptile and hypocotyl of weeds, and has an inhibitory effect on the growth of young buds and secondary roots, preventing cell division, resulting in weeds being unable to emerge normally or growing stunted and dying. Imazethapyr is an ALS (acetolactate synthase) or AHAS (acetohydroxyacid synthase) inhibitor, which can effectively prevent the biosynthesis of amino acids (such as valine, leucine, and isoleucine) in plants, interfere with the normal metabolism of plants, and ultimately lead to inhibited plant growth until death. It has high selectivity, especially suitable for leguminous crop fields, such as peanut fields and soybean fields. It can effectively kill gramineous and broad - leaf weeds, with a wide herbicidal spectrum. It can not only control annual gramineous weeds, such as crabgrass and green foxtail, but also control perennial gramineous weeds such as Imperata cylindrica. At the same time, it is also effective against a variety of broad - leaf weeds. Compared with other types of herbicides, imazethapyr is a low - toxicity herbicide, relatively safe for the environment and human body.

[0007] Among the existing types of pesticides, how to reduce toxicity and expand the control effect through compounding has always been the focus of attention of scientific research personnel. Therefore, developing a pesticide product that can meet the above effects has high market value. Summary of the Invention

[0008] The purpose of the present invention is to obtain a new type of herbicide product with low toxicity but excellent effects.

[0009] To achieve the above purpose, in the first aspect of the present invention, a soil - closed herbicide for alfalfa fields is provided. The active ingredients of the herbicide include: acetochlor and trifluralin. Among them, the mass concentration of acetochlor in the herbicide is 10 - 30%, and the mass concentration of trifluralin in the herbicide is 10 - 20%.

[0010] Or,

[0011] The herbicide active ingredient includes: acetochlor and imazethapyr. Among them, the mass concentration of acetochlor in the herbicide is 10 - 30%, and the mass concentration of imazethapyr in the herbicide is 15 - 30%.

[0012] Through innovative formulation design, the present invention has achieved a breakthrough in synergistically improving the weeding effect and ecological safety. Experimental data shows that with the herbicide active ingredient system with a specific concentration ratio of the present invention, while maintaining a plant control efficacy of over 95%, the toxicity of the agent is significantly reduced. Notably, the alfalfa planting area where the product of the present invention is applied shows significant growth advantages:

[0013] 1. The emergence rate is increased by about 85%;

[0014] 2. The average plant height is increased by about 76%;

[0015] 3. The biomass accumulation is increased by about 21%;

[0016] This technical characteristic of high efficiency and low toxicity provides a safer weed control solution for agricultural production.

[0017] Furthermore, the herbicide of the present invention also includes active peptides, and the mass concentration of the active peptides in the herbicide is 2 - 5%. In modern agricultural production, acetochlor and trifluralin / imazethapyr, as common selective herbicides, are widely used in farmland weed control. However, the soil residue problem of the above-mentioned original drugs in traditional use has always been a bottleneck restricting crop rotation and ecological safety. Acetochlor, as an amide herbicide, usually has a persistence period of 8 - 10 weeks (about 56 - 70 days) in the soil, and its residue can affect the growth of subsequent crops through root absorption; trifluralin, as a dinitroaniline herbicide, has a half-life of up to 57 - 126 days in the soil and is easily adsorbed by soil particles, resulting in an extended residue period of 2 - 4 months. Imazethapyr is an imidazolinone herbicide, usually with a half-life of 60 - 90 days in the soil. After using the above-mentioned original drug herbicides, it will affect the subsequent growth of alfalfa, such as the plant height and alfalfa biomass accumulation not reaching excellent indicators. The present invention innovatively adds active peptides to the original drug, which can significantly accelerate the soil degradation process of the above three original drugs, minimizing the adverse effects of the herbicide on the subsequent growth of alfalfa, thereby ensuring high-quality alfalfa crops.

[0018] Preferably, the active peptide is an active peptide obtained by enzymatically hydrolyzing soybeans and peas. Specifically, the soy active peptide is an antioxidant peptide, and its amino acid sequence is as shown in SEQ ID NO.1; the pea active peptide is a pea oligopeptide, and its amino acid sequence is as shown in SEQ ID NO.2. The present invention preferably uses soy antioxidant peptide and pea oligopeptide. The soy antioxidant peptide has been experimentally verified to have a significant cardiovascular protection effect, can regulate blood pressure by inhibiting the activity of angiotensin-converting enzyme, and at the same time has biological activities such as improving glucose and lipid metabolism and enhancing macrophage phagocytosis function. The pea oligopeptide is prepared by a unique enzymatic hydrolysis process, and its rich hydrophobic amino acid sequence can effectively inhibit the activity of ACE enzyme, showing a dose-dependent effect in reducing blood pressure, and at the same time can promote the proliferation of spleen lymphocytes. The present invention creatively and scientifically compounded the above-mentioned active peptides originally used in the biomedical and food grades with acetochlor, trifluralin / imazethapyr, significantly improving the degradation efficiency of the above-mentioned original drugs and minimizing the impact of chemical agents on the subsequent growth of plants.

[0019] Furthermore, based on the key physicochemical properties such as storage stability and solubility of the above three herbicide original drugs, the present invention specifically developed a special adjuvant system for compounding and using with them. The herbicide provided by the present invention can be made into various dosage forms, such as emulsifiable concentrate, wettable powder, suspension concentrate, dispersible oil suspension, water dispersible granule.

[0020] Specifically, when the herbicide dosage form is emulsifiable concentrate, the adjuvant consists of calcium dodecylbenzenesulfonate, isomeric alcohol polyoxyethylene ether, and toluene. Among them, the mass percentage of calcium dodecylbenzenesulfonate in the adjuvant is 10-25%, the mass percentage of isomeric alcohol polyoxyethylene ether in the adjuvant is 10-25%, and the balance is toluene;

[0021] When the herbicide dosage form is wettable powder, the adjuvant consists of sodium lignosulfonate, silicone adjuvant, diatomite, naphthalene sulfonate formaldehyde condensate, pregelatinized starch, and DK8 suspension agent. Among them, the mass percentage of sodium lignosulfonate in the adjuvant is 1-5%, the mass percentage of silicone adjuvant in the adjuvant is 1-3%, the mass percentage of naphthalene sulfonate formaldehyde condensate in the adjuvant is 0.5-2%, the mass percentage of pregelatinized starch in the adjuvant is 0.5-2%, and the mass percentage of DK8 suspension agent in the adjuvant is 3-10%, and the balance is diatomite;

[0022] When the herbicide dosage form is suspension concentrate, the adjuvant consists of sodium lignosulfonate, sodium dodecyl sulfate, and water. Among them, the mass percentage of sodium lignosulfonate in the adjuvant is 30-40%, the mass percentage of sodium dodecyl sulfate in the adjuvant is 20-30%, and the balance is water;

[0023] When the herbicide formulation is a dispersible oil suspension, the adjuvant consists of sodium lignosulfonate, xanthan gum, dinonylphenol polyoxyethylene ether, ethylene glycol, and methyl oleate. Among them, the mass percentage of sodium lignosulfonate in the adjuvant is 5-10%, the mass percentage of xanthan gum in the adjuvant is 1-5%, the mass percentage of dinonylphenol polyoxyethylene ether in the adjuvant is 5-10%, the mass percentage of ethylene glycol in the adjuvant is 5-15%, and the balance is methyl oleate;

[0024] When the herbicide formulation is a water dispersible granule, the adjuvant consists of DS707, sodium dodecyl sulfate, modified starch, and diatomite. Among them, the mass percentage of DS707 in the adjuvant is 10-20%, the mass percentage of sodium dodecyl sulfate in the adjuvant is 15-25%, the mass percentage of modified starch in the adjuvant is 10-25%, and the balance is diatomite.

[0025] When optimizing the formulation of the present invention, key technical indicators such as the chemical compatibility between the technical herbicide and the active peptide, the long-term stability of the formulation, biological safety, and crop protection efficacy were systematically considered. After scientific verification, the selected adjuvant system is the optimal selection.

[0026] On the other hand, the present invention provides the use of the herbicide. Specifically, the herbicide of the present invention is applied in alfalfa fields to control Chenopodiaceae, Amaranthaceae, and Gramineae weeds. It is well known to those skilled in the art that Chenopodiaceae, Amaranthaceae, and Gramineae weeds in alfalfa fields are the most difficult to control weed species. The novel herbicide provided by the present invention can achieve a control effect of more than 95% on the above weeds while ensuring safety.

[0027] Specifically, the application rate of the above herbicide is 250-500 ml / hm 2 , which is nearly half less than the application rate of the herbicides containing acetochlor, trifluralin, and imazethapyr in the prior art. With a low application rate, it has little impact on the soil and can achieve sustainable development, but the weeding efficiency is not reduced and still remains at a high level.

[0028] Compared with the prior art, the present invention has the following beneficial effects:

[0029] 1. Synergistic weeding system: Through the binary compounding of acetochlor and trifluralin, or the binary compounding of acetochlor and imazethapyr, combined with precisely optimized formulation parameters and field application rates, while ensuring the emergence rate of crops and significantly improving the seedling growth indicators (plant height, root length, biomass accumulation), the control effect on the same weed population can reach more than 95%.

[0030] 2. Environmentally friendly degradation system: Innovatively introducing active peptides significantly improves the degradation efficiency of the technical herbicide and has no adverse effects on the subsequent growth of crops.

[0031] 3. The herbicide composition provided by the present invention can maintain excellent weed control efficacy while reducing the application rate per unit area through the synergistic effect of the innovative compounding adjuvant system and the technical drug. It solves the technical bottlenecks such as easy stratification and crystallization during storage and viscosity increase during transportation of traditional formulations. After the accelerated stability test, the qualification rate of each index of the formulation remains 100%. While achieving reduced application, the herbicide composition significantly improves the physical and chemical stability and the convenience of use of the product, and has important popularization and application value. Detailed implementation manners

[0032] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0033] Example 1

[0034] This example provides a soil-sealing herbicide for alfalfa fields. The active ingredients of the herbicide are acetochlor and trifluralin. Among them, the mass concentration of acetochlor in the herbicide is 10%, and the mass concentration of trifluralin in the herbicide is 20%. Water is used as a solvent to mix with the two technical drugs to obtain the herbicide, and the application rate is 500 mL / hm 2 , and it can be used in alfalfa fields by conventional methods such as spraying and sprinkling in the prior art to control Chenopodiaceae, Amaranthaceae, and Gramineae weeds.

[0035] Example 2

[0036] A soil-sealing herbicide for alfalfa fields provided by the present invention. The active ingredients of the herbicide are acetochlor and imazethapyr. Among them, the mass concentration of acetochlor in the herbicide is 30%, and the mass concentration of imazethapyr in the herbicide is 15%. The balance is water, and the herbicide is prepared. The application rate is 450 mL / hm 2 , and it can be used in alfalfa fields by conventional methods such as spraying and sprinkling in the prior art to control Chenopodiaceae, Amaranthaceae, and Gramineae weeds.

[0037] Example 3

[0038] This example provides a soil - closed herbicide. The active ingredients of the herbicide are a compound of acetochlor, trifluralin, and active peptide. Among them, the mass concentration of acetochlor in the herbicide is 20%, the mass concentration of trifluralin in the herbicide is 15%, and the mass concentration of active peptide in the herbicide is 2%. The balance is water to prepare the herbicide. The active peptide used is soybean antioxidant peptide, and its amino acid sequence is as shown in SEQ ID NO.1 (the specific amino acid sequence is Gln - Arg - Pro - Arg, and the specific amino acid sequences of the soybean antioxidant peptides in the examples of the present invention are all the same as those in Example 3), purchased from Baiying Biological Company, and the application rate is 350 mL / hm 2 , and it can be used in alfalfa fields by conventional methods such as spraying and sprinkling in the prior art to control Chenopodiaceae, Amaranthaceae, and Gramineae weeds.

[0039] Example 4

[0040] This example provides a soil - closed herbicide. The active ingredients of the herbicide are a compound of acetochlor, imazethapyr, and active peptide. Among them, the mass concentration of acetochlor in the herbicide is 15%, the mass concentration of imazethapyr in the herbicide is 30%, and the mass concentration of active peptide in the herbicide is 5%. The balance is water to prepare the herbicide. The active peptide used is pea oligopeptide, and its amino acid sequence is as shown in SEQ ID NO.2, (the specific amino acid sequence is Lys - Trp - Phe - Met - Thr - Ile - Leu - Val, and the amino acid sequences of the pea oligopeptides in the examples of the present invention are all the same as those in Example 4), purchased from Xi'an Dongfeng Biotechnology Co., Ltd., and the application rate is 350 mL / hm 2 , and it can be used in alfalfa fields by conventional methods such as spraying and sprinkling in the prior art to control Chenopodiaceae, Amaranthaceae, and Gramineae weeds.

[0041] Example 5

[0042] This example provides a soil - closed herbicide with an emulsifiable concentrate formulation. The active ingredients of the herbicide are a compound of acetochlor, trifluralin, and active peptide. Among them, the mass concentration of acetochlor in the herbicide is 25%, the mass concentration of trifluralin in the herbicide is 10%, and the mass concentration of active peptide in the herbicide is 3%. The balance is an adjuvant. The adjuvant consists of calcium dodecylbenzenesulfonate, isomeric alcohol polyoxyethylene ether, and toluene. Among them, the mass percentage of calcium dodecylbenzenesulfonate in the adjuvant is 25%, the mass percentage of isomeric alcohol polyoxyethylene ether in the adjuvant is 25%, and the balance is toluene. The active peptide used is pea oligopeptide, and its amino acid sequence is as shown in SEQ ID NO.2, purchased from Xi'an Dongfeng Biotechnology Co., Ltd. The application rate is 200 mL / hm 2, The preparation method of the emulsifiable concentrate formulation can be prepared by using the existing technology and is used in alfalfa fields in conventional ways such as spraying and sprinkling of the existing technology to control Chenopodiaceae, Amaranthaceae, and Gramineae weeds.

[0043] Example 6

[0044] This example provides a soil closed herbicide with a wettable powder formulation. The active ingredients of the herbicide are a compound of acetochlor, imazethapyr, and active peptide. Among them, the mass concentration of acetochlor in the herbicide is 16%, the mass concentration of imazethapyr in the herbicide is 25%, and the mass concentration of active peptide in the herbicide is 3%. The balance is auxiliaries, and the auxiliaries are composed of sodium lignosulfonate, silicone-based auxiliary, diatomaceous earth, naphthalene sulfonate formaldehyde condensate, pregelatinized starch, and DK8 suspension agent. Among them, the mass percentage of sodium lignosulfonate in the auxiliaries is 1%, the mass percentage of silicone-based auxiliary in the auxiliaries is 3%, the mass percentage of naphthalene sulfonate formaldehyde condensate in the auxiliaries is 0.5%, the mass percentage of pregelatinized starch in the auxiliaries is 0.5 - %, the mass percentage of DK8 suspension agent in the auxiliaries is 3%, and the balance is diatomaceous earth. The active peptide used is soy antioxidant peptide, and its amino acid sequence is as shown in SEQ ID NO.1, purchased from Baiying Biology. Mix the wettable powder herbicide of the present invention with water according to a mass ratio of 1:500 - 1500 and stir evenly. The application rate is 250 mL / hm 2 , It can be used in alfalfa fields in conventional ways such as spraying and sprinkling of the existing technology to control Chenopodiaceae, Amaranthaceae, and Gramineae weeds. The preparation method of the wettable powder can be prepared by using the existing technology.

[0045] Example 7

[0046] This example provides a soil closed herbicide with a suspension formulation. The active ingredients of the herbicide are a compound of acetochlor, trifluralin, and active peptide. Among them, the mass concentration of acetochlor in the herbicide is 29%, the mass concentration of trifluralin in the herbicide is 11%, and the mass concentration of active peptide in the herbicide is 4%. The balance is auxiliaries, and the auxiliaries are composed of sodium lignosulfonate, sodium dodecyl sulfate, and water. Among them, the mass percentage of sodium lignosulfonate in the auxiliaries is 30%, the mass percentage of sodium dodecyl sulfate in the auxiliaries is 20%, and the balance is water. The active peptide used is pea oligopeptide, and its amino acid sequence is as shown in SEQ ID NO.2, purchased from Xi'an Dongfeng Biotechnology Co., Ltd. The application rate is 300 mL / hm 2 , It can be used in alfalfa fields in conventional ways such as spraying and sprinkling of the existing technology to control Chenopodiaceae, Amaranthaceae, and Gramineae weeds. The preparation method of the suspension can be prepared by using the existing technology.

[0047] Example 8

[0048] This example provides a soil - closed herbicide with a dispersible oil suspension formulation. The active ingredients of the herbicide are a compound of acetochlor, imazethapyr, and active peptide. Among them, the mass concentration of acetochlor in the herbicide is 12%, the mass concentration of imazethapyr in the herbicide is 16%, and the mass concentration of active peptide in the herbicide is 5%. The balance is auxiliaries. The auxiliaries are composed of sodium lignosulfonate, xanthan gum, dinonylphenol polyoxyethylene ether, ethylene glycol, and methyl oleate. Among them, the mass percentage of sodium lignosulfonate in the auxiliaries is 5%, the mass percentage of xanthan gum in the auxiliaries is 1%, the mass percentage of dinonylphenol polyoxyethylene ether in the auxiliaries is 5%, the mass percentage of ethylene glycol in the auxiliaries is 5%, and the balance is methyl oleate. The active peptide used is an antioxidant peptide, and its amino acid sequence is as shown in SEQ ID NO.1, purchased from Baiying Biology. The application rate is 200 mL / hm 2 , and it can be used in alfalfa fields by conventional methods such as spraying and sprinkling in the prior art, for controlling Chenopodiaceae, Amaranthaceae, and Gramineae weeds. The preparation method of the dispersible oil suspension can be prepared by the prior art.

[0049] Example 9

[0050] This example provides a soil - closed herbicide with a water - dispersible granule formulation. The active ingredients of the herbicide are a compound of acetochlor, trifluralin, and active peptide. Among them, the mass concentration of acetochlor in the herbicide is 28%, the mass concentration of trifluralin in the herbicide is 19%, and the mass concentration of active peptide in the herbicide is 3.5%. The balance is auxiliaries. The auxiliaries are composed of DS707, sodium dodecyl sulfate, modified starch, and diatomite. Among them, the mass percentage of DS707 in the auxiliaries is 10%, the mass percentage of sodium dodecyl sulfate in the auxiliaries is 15%, the mass percentage of modified starch in the auxiliaries is 10%, and the balance is diatomite. The active peptide used is pea oligopeptide, and its amino acid sequence is as shown in SEQ ID NO.2, purchased from Xi'an Dongfeng Biotechnology Co., Ltd. The herbicide with the water - dispersible granule formulation of the present invention is mixed and stirred evenly with water at a mass ratio of 1:100 - 1000, and the application rate is 250 mL / hm 2 , and it can be used in alfalfa fields by conventional methods such as spraying and sprinkling in the prior art, for controlling Chenopodiaceae, Amaranthaceae, and Gramineae weeds. The water - dispersible granule can be prepared by the prior art.

[0051] Example 10

[0052] This embodiment provides a soil - closed herbicide with an emulsifiable concentrate dosage form. The active ingredients of the herbicide are a compound of acetochlor, trifluralin, and active peptide. Among them, the mass concentration of acetochlor in the herbicide is 27%, the mass concentration of trifluralin in the herbicide is 12%, and the mass concentration of active peptide in the herbicide is 4.5%. The balance is auxiliary agents. The auxiliary agents are composed of calcium dodecylbenzenesulfonate, isomeric alcohol polyoxyethylene ether, and toluene. Among them, the mass percentage of calcium dodecylbenzenesulfonate in the auxiliary agents is 10%, the mass percentage of isomeric alcohol polyoxyethylene ether in the auxiliary agents is 10%, and the balance is toluene. The active peptide used is pea oligopeptide, and its amino acid sequence is shown in SEQ ID NO.2, which is purchased from Xi'an Dongfeng Biotechnology Co., Ltd. The application rate is 300 mL / hm 2 , and the preparation method of the emulsifiable concentrate dosage form can be prepared by existing technologies. In alfalfa fields, it is used in conventional ways such as spraying and sprinkling of existing technologies to control Chenopodiaceae, Amaranthaceae, and Gramineae weeds.

[0053] Example 11

[0054] This embodiment provides a soil - closed herbicide with a wettable powder dosage form. The active ingredients of the herbicide are a compound of acetochlor, imazethapyr, and active peptide. Among them, the mass concentration of acetochlor in the herbicide is 14%, the mass concentration of imazethapyr in the herbicide is 28%, and the mass concentration of active peptide in the herbicide is 3%. The balance is auxiliary agents. The auxiliary agents are composed of sodium lignosulfonate, silicone auxiliary agent, diatomite, naphthalene sulfonate formaldehyde condensate, pre - gelatinized starch, and DK8 suspending agent. Among them, the mass percentage of sodium lignosulfonate in the auxiliary agents is 5%, the mass percentage of silicone auxiliary agent in the auxiliary agents is 1%, the mass percentage of naphthalene sulfonate formaldehyde condensate in the auxiliary agents is 2%, the mass percentage of pre - gelatinized starch in the auxiliary agents is 2%, the mass percentage of DK8 suspending agent in the auxiliary agents is 10%, and the balance is diatomite. The active peptide used is soybean antioxidant peptide, and its amino acid sequence is shown in SEQ ID NO.1, which is purchased from Baiying Biology. The wettable powder herbicide of the present invention is mixed and stirred evenly with water according to a mass ratio of 1:500 - 1500, and the application rate is 290 mL / hm 2 , and it can be used in alfalfa fields in conventional ways such as spraying and sprinkling of existing technologies. It is used to control Chenopodiaceae, Amaranthaceae, and Gramineae weeds, and the preparation method of the wettable powder can be prepared by existing technologies.

[0055] Example 12

[0056] This embodiment provides a soil - closed herbicide with a dosage form of a dispersible oil suspension. The active ingredients of the herbicide are a compound of acetochlor, imazethapyr, and an active peptide. Among them, the mass concentration of acetochlor in the herbicide is 18%, the mass concentration of imazethapyr in the herbicide is 21%, the mass concentration of the active peptide in the herbicide is 5%, and the balance is auxiliary agents. The auxiliary agents are composed of sodium lignosulfonate, xanthan gum, dinonylphenol polyoxyethylene ether, ethylene glycol, and methyl oleate. Among them, the mass percentage of sodium lignosulfonate in the auxiliary agents is 10%, the mass percentage of xanthan gum in the auxiliary agents is 5%, the mass percentage of dinonylphenol polyoxyethylene ether in the auxiliary agents is 10%, the mass percentage of ethylene glycol in the auxiliary agents is 15%, and the balance is methyl oleate. The active peptide used is an antioxidant peptide, and its amino acid sequence is shown in SEQ ID NO.1, which is purchased from Baiying Biology. The application rate is 200 mL / hm 2 , and it can be used in alfalfa fields by conventional methods such as spraying and sprinkling in the prior art. It is used to control Chenopodiaceae, Amaranthaceae, and Gramineae weeds, and the preparation method of the dispersible oil suspension can be prepared by the prior art.

[0057] Example 13

[0058] This embodiment provides a soil - closed herbicide with a dosage form of a water - dispersible granule. The active ingredients of the herbicide are a compound of acetochlor, trifluralin, and an active peptide. Among them, the mass concentration of acetochlor in the herbicide is 17%, the mass concentration of trifluralin in the herbicide is 14%, the mass concentration of the active peptide in the herbicide is 4.5%, and the balance is auxiliary agents. The auxiliary agents are composed of DS707, sodium dodecyl sulfate, modified starch, and diatomite. Among them, the mass percentage of DS707 in the auxiliary agents is 20%, the mass percentage of sodium dodecyl sulfate in the auxiliary agents is 25%, the mass percentage of modified starch in the auxiliary agents is 25%, and the balance is diatomite. The active peptide used is pea oligopeptide, and its amino acid sequence is shown in SEQ ID NO.2, which is purchased from Xi'an Dongfeng Biotechnology Co., Ltd. The water - dispersible granule herbicide of the present invention is stirred and mixed with water according to a mass ratio of 1:100 - 1000, and the application rate is 280 mL / hm 2 , and it can be used in alfalfa fields by conventional methods such as spraying and sprinkling in the prior art. It is used to control Chenopodiaceae, Amaranthaceae, and Gramineae weeds. The water - dispersible granule can be prepared by the prior art.

[0059] The beneficial effects achieved by the technical solution of the present invention are specifically described through experiments as follows:

[0060] I. Set Comparative Examples 1 - 10 for comparison with Examples 1 - 9 of the present invention:

[0061] Comparative Example 1: Acetochlor, S-metolachlor, and water were compounded to obtain a herbicide. The mass concentration of acetochlor in the herbicide was 25%, the mass concentration of S-metolachlor in the herbicide was 40%, and the balance was water. Mix them to obtain the herbicide.

[0062] Comparative Example 2: Pendimethalin, trifluralin, and water were compounded to obtain a herbicide. The mass concentration of pendimethalin in the herbicide was 30%, the mass concentration of trifluralin in the herbicide was 30%, and the balance was water. Mix them to obtain the herbicide.

[0063] Comparative Example 3: S-metolachlor, imazethapyr, and water were compounded to obtain a herbicide. The mass concentration of S-metolachlor in the herbicide was 30%, the mass concentration of imazethapyr in the herbicide was 20%, and the balance was water. Mix them to obtain the herbicide.

[0064] Comparative Example 4: Acetochlor, trifluralin, corn oligopeptide, and water were mixed and compounded to obtain a herbicide. The mass concentration of acetochlor in the herbicide was 20%, the mass concentration of trifluralin in the herbicide was 15%, and the mass concentration of corn oligopeptide in the herbicide was 2%. The corn oligopeptide was purchased from Xi'an Entaiyuan Biotechnology Co., Ltd., and the balance was water.

[0065] Comparative Example 5: Acetochlor, imazethapyr, wheat oligopeptide, and water were mixed and compounded to obtain a herbicide. The mass concentration of acetochlor in the herbicide was 15%, the mass concentration of imazethapyr in the herbicide was 30%, and the concentration of wheat oligopeptide in the herbicide was 5%. The balance was water. The wheat oligopeptide was purchased from Shanxi Wenyun Biotechnology Co., Ltd.

[0066] Comparative Example 6: Acetochlor, trifluralin, pea oligopeptide (the pea oligopeptide and soybean antioxidant peptide used in the comparative example were the same as those in the example), biodiesel, dodecylaminopropionic acid, and toluene were made into an emulsifiable concentrate. The mass percentages of the above components in the herbicide were 25% acetochlor, 10% trifluralin, and 3% pea oligopeptide. The balance was the auxiliary agent. The auxiliary agent was composed of biodiesel, dodecylaminopropionate, and toluene. The mass percentage of biodiesel in the auxiliary agent was 30%, the mass percentage of dodecylaminopropionate in the auxiliary agent was 25%, and the balance was toluene. It was processed by the existing preparation technology of emulsifiable concentrate.

[0067] Comparative Example 7: Acetochlor, imazethapyr, soybean antioxidant peptide, and an auxiliary agent were made into a wettable powder herbicide. The mass concentration of acetochlor in the herbicide was 16%, the mass concentration of imazethapyr in the herbicide was 25%, and the mass concentration of soybean antioxidant peptide in the herbicide was 3%. The balance was the auxiliary agent. The auxiliary agent was composed of sulfonated castor oil with a mass concentration of 15%, sodium lignosulfonate with a mass concentration of 10%, nonylphenol polyoxyethylene ether with a mass concentration of 5%, and the balance was soybean oil. It was processed by the existing preparation technology of wettable powder.

[0068] Comparative Example 8: Acetochlor, trifluralin, pea oligopeptide, and an adjuvant were made into a suspension-type herbicide. The mass concentration of acetochlor in the herbicide was 29%, the mass concentration of trifluralin in the herbicide was 11%, the mass concentration of pea oligopeptide in the herbicide was 4%, and the balance was the adjuvant. The adjuvant was composed of alkylnaphthalenesulfonate, sodium polycarboxylate, ethylene glycol, and water. Among them, the mass percentage of alkylnaphthalenesulfonate in the adjuvant was 30%, the mass percentage of sodium polycarboxylate in the adjuvant was 20%, the mass percentage of ethylene glycol in the adjuvant was 30%, and the balance was water. It was processed by the existing preparation technology of suspending agents.

[0069] Comparative Example 9: Acetochlor, imazethapyr, soybean antioxidant peptide, and an adjuvant were made into a dispersible oil suspension-type herbicide. The mass concentration of acetochlor in the herbicide was 12%, the mass concentration of imazethapyr in the herbicide was 16%, the mass concentration of soybean antioxidant peptide in the herbicide was 5%, and the balance was the adjuvant. The adjuvant was composed of polyoxyalkylene castor oil, sodium dialkylsulfosuccinate, bentonite, and methyl oleate. Among them, the mass percentage of polyoxyalkylene castor oil in the adjuvant was 15%, the mass percentage of sodium dialkylsulfosuccinate in the adjuvant was 10%, the mass percentage of bentonite in the adjuvant was 5%, and the balance was methyl oleate. It was processed by the existing preparation technology of dispersible oil suspensions.

[0070] Comparative Example 10: Acetochlor, trifluralin, pea oligopeptide, and an adjuvant were made into a water-dispersible granule-type herbicide. The mass concentration of acetochlor in the herbicide was 28%, the mass concentration of trifluralin in the herbicide was 19%, the mass concentration of pea oligopeptide in the herbicide was 3.5%, and the balance was the adjuvant. The adjuvant was composed of alkylaryl polyoxyethylene ether, saponin powder, aluminum chloride, carboxymethyl cellulose, and kaolin. Among them, the mass percentage of alkylaryl polyoxyethylene ether in the adjuvant was 10%, the mass percentage of saponin powder in the adjuvant was 15%, the mass percentage of aluminum chloride in the adjuvant was 10%, the mass percentage of carboxymethyl cellulose in the adjuvant was 10%, and the balance was kaolin. It was processed by the existing preparation technology of water-dispersible granules. It should be noted that when the wettable powder and the water-dispersible granules in the comparative examples were used, the mass ratio of mixing with water was the same as that in the examples of the present invention.

[0071] II. Experiments on the stability of Examples 1-9 of the present invention and Comparative Examples 1-10 were carried out, and each group had 3 parallel repeated experiments:

[0072] 1. Experimental method:

[0073] (1) Thermal storage stability test: Place each group of herbicides in a stoppered graduated test tube with good sealing, and place it in a water bath at 54 ± 0.1 °C for 14 days of thermal storage. After thermal storage, if the system is uniform and the water separation rate is less than 10%, it is qualified; otherwise, it is unqualified.

[0074] (2) Cold storage stability test: Place the herbicide in a stoppered graduated test tube, then freeze it at -18 °C in the refrigerator for 24 h, let it thaw automatically at room temperature, and then place it back in the refrigerator, repeating this process ten times. Observe whether there is any substance precipitation or flocculent precipitate. If there is, it is unqualified; otherwise, it is qualified.

[0075] 2. The experimental results are shown in the following table:

[0076] Group Example 1 Example 2 Comparative Example 1 Comparative Example 2 Comparative Example 3 Hot storage stability Unqualified Unqualified Unqualified Unqualified Unqualified Cold storage stability Unqualified Unqualified Unqualified Unqualified Unqualified

[0077]

[0078] Although the group with the added polypeptide is superior to the group without the added polypeptide in terms of water separation rate and substance precipitation, the stability still fails the stability test.

[0079]

[0080] From the above experimental results, it can be seen that the herbicide with the added adjuvant is superior to other groups in terms of stability performance, and the stability performance is qualified.

[0081] III. Field experiments were conducted on Examples 1 - 9 and Comparative Examples 1 - 10 of the present invention. During the field experiment, clear water and manual weeding controls were set. It should be noted that all groups in the field experiment were conducted with 3 parallel repeated experiments, and the obtained data were average data to specifically illustrate the beneficial technical effects of the herbicide obtained by the technical solution of the present invention, namely low toxicity and high control rate.

[0082] 1. Experimental method: (1) Field design: Alfalfa was sown on July 9, 2022, in the experimental field of Hohhot City using a push-type seeder, with a seeding rate of 22.5 kg / hm 2 , and on July 12, 2022, in the experimental field of Ulanqab City using a large special seeder, with a seeding rate of 15 kg / hm 2 . The row spacing of alfalfa is 25 cm, the seeding depth of the seeds is 1 - 2 cm, the plot area is 3 m × 5 m. After sowing, immediately spray the herbicide using a pneumatic sprayer with a capacity of 3 L, and calculate the required herbicide and water volume within the specified area according to the herbicide usage instructions. A randomized block design was adopted, with 3 replicates, and manual weeding control (manual weeding once a week for weeds) and blank control with clear water treatment were set.

[0083] (2) Safety evaluation of alfalfa

[0084] Select 0.25 m² quadrats in each plot 2 randomly three times. After 14 days of applying the drug, investigate the number of alfalfa seedlings, after 30 days, investigate the emergence number, measure the plant height and fresh weight of the plants, and then put them into mesh bags, hang them to dry, and measure the dry weight of alfalfa.

[0085] (3) Investigation of weed control efficacy

[0086] Select 0.25 m 2 ² quadrats in each plot, randomly three times. After 30 days of applying the drug, investigate the plant control efficacy of weeds;

[0087] Plant control efficacy (%) = (number of weed plants in the blank control plot - number of weed plants in the treated plot) / number of weed plants in the blank control plot × 100. The weeds to be controlled are Chenopodiaceae, Amaranthaceae, and Gramineae weeds;

[0088] 2. The experimental results are as follows:

[0089] Group Example 1 Example 2 Comparative Example 1 Comparative Example 2 Comparative Example 3 Emergence rate (%) 65 63 35 38 40 Plant height (cm) 23 25 13 11 15 <![CDATA[Fresh weight of alfalfa (kg / 667m 2 )]]> 440 450 396 380 385 <![CDATA[Dry weight of alfalfa (kg / 667m 2 )]]> 92 93 76 68 71 Plant control efficacy (%) 95 96 90 89 91 <![CDATA[Application rate (mL / hm 2 )]]> 500 450 850 800 900

[0090] Group Example 3 Example 4 Comparative Example 4 Comparative Example 5 Emergence rate (%) 70 69 42 44 Plant height (cm) 23 24 15 17 <![CDATA[Fresh weight of alfalfa (kg / 667m 2 )]]> 470 465 385 390 <![CDATA[Alfalfa dry weight (kg / 667m 2 )]]> 95 94 65 68 Plant control efficacy (%) 96 97 93 94 <![CDATA[Application rate (mL / hm 2 )]]> 350 350 750 750

[0091]

[0092]

[0093] Group Comparative Example 6 Comparative Example 7 Comparative Example 8 Comparative Example 9 Comparative Example 10 Emergence rate (%) 51 53 55 49 52 Plant height (cm) 15 17 16 13 15 <![CDATA[Fresh weight of alfalfa (kg / 667m 2 )]]> 390 392 395 388 398 <![CDATA[Dry weight of alfalfa (kg / 667m 2 )]]> 71 68 73 63 72 Plant control efficacy (%) 95 93 91 94 93 <![CDATA[Application rate (mL / hm 2 )]]> 1000 950 900 1050 960

[0094] Group Water control Manual weeding Emergence rate (%) 73 77 Plant height (cm) 24 29 <![CDATA[Fresh weight of alfalfa (kg / 667m 2 )]]> 408 81 <![CDATA[Dry weight of alfalfa (kg / 667m 2 )]]> 496 102 Plant control efficacy (%) - 100

[0095] The present invention also adopts the above-mentioned field experimental method to control other weeds, such as broad-leaved weeds, monocotyledonous weeds, etc., but the plant control efficacy is all lower than 80%. At the same time, from the above experimental results, it can be seen that only by using the specific herbicide active ingredients, specific mass ratio relationships, specific polypeptides, and specific auxiliaries of the present invention can a herbicide with a plant control efficacy above 95% and high safety be comprehensively obtained.

[0096] The above is the preferred embodiment of the present invention. It should be noted that for those of ordinary skill in the art, without departing from the principle described in the present invention, several improvements and refinements can be made, and these improvements and refinements should also be regarded as the protection scope of the present invention.

Claims

1. A soil-sealing herbicide for alfalfa fields, characterized in that: The active ingredients of the herbicide include: acetochlor and trifluralin, wherein the mass concentration of acetochlor in the herbicide is 10-30%, and the mass concentration of trifluralin in the herbicide is 10-20%; or, The active ingredients of the herbicide include: acetochlor and imazethapyr, wherein the mass concentration of acetochlor in the herbicide is 10-30%, and the mass concentration of imazethapyr in the herbicide is 15-30%.

2. The herbicide according to claim 1, characterized in that The active ingredients also include active peptides, and the mass concentration of the active peptides in the herbicide is 2-5%.

3. The herbicide according to claim 2, characterized in that The active peptides are obtained by enzymatic hydrolysis of soybeans and peas.

4. The herbicide according to claim 3, characterized in that The soybean active peptide is an antioxidant peptide, and its amino acid sequence is shown in SEQ ID NO.

1.

5. The herbicide according to claim 3, characterized in that The pea active peptide is a pea oligopeptide, and its amino acid sequence is shown in SEQ ID NO.

2.

6. The herbicide according to any one of claims 1 to 5, characterized in that The herbicide consists of the active ingredient and the balance is herbicide adjuvant.

7. The herbicide according to claim 6, characterized in that The herbicide formulation is any one of emulsifiable concentrate, wettable powder, suspension, dispersible oil suspension, and water dispersible granules.

8. The herbicide according to claim 7, characterized in that When the herbicide formulation is emulsifiable concentrate, the adjuvant is composed of calcium dodecylbenzene sulfonate, isomeric alcohol polyoxyethylene ether, and toluene, wherein the mass percentage of calcium dodecylbenzene sulfonate in the adjuvant is 10-25%, the mass percentage of isomeric alcohol polyoxyethylene ether in the adjuvant is 10-25%, and the balance is toluene; When the herbicide formulation is a wettable powder, the adjuvant is composed of sodium lignin sulfonate, an organosilicon adjuvant, diatomaceous earth, a naphthalene sulfonate formaldehyde condensate, pregelatinized starch, and a DK8 disintegrating suspending agent, wherein the mass percentage of sodium lignin sulfonate in the adjuvant is 1-5%, the mass percentage of the organosilicon adjuvant in the adjuvant is 1-3%, the mass percentage of the naphthalene sulfonate formaldehyde condensate in the adjuvant is 0.5-2%, the mass percentage of the pregelatinized starch in the adjuvant is 0.5-2%, the mass percentage of the DK8 disintegrating suspending agent in the adjuvant is 3-10%, and the balance is diatomaceous earth; When the herbicide formulation is a suspension concentrate, the adjuvant is composed of sodium lignin sulfonate, sodium lauryl sulfate, and water, wherein the mass percentage of sodium lignin sulfonate in the adjuvant is 30-40%, the mass percentage of sodium lauryl sulfate in the adjuvant is 20-30%, and the balance is water; When the herbicide formulation is a dispersible oil suspension, the adjuvant is composed of sodium lignin sulfonate, xanthan gum, dinonylphenol polyoxyethylene ether, ethylene glycol, and methyl oleate, wherein the mass percentage of sodium lignin sulfonate in the adjuvant is 5-10%, the mass percentage of xanthan gum in the adjuvant is 1-5%, the mass percentage of dinonylphenol polyoxyethylene ether in the adjuvant is 5-10%, and the mass percentage of ethylene glycol in the adjuvant is 5-15%. The balance is methyl oleate; When the herbicide formulation is a water dispersible granule, the adjuvant is composed of DS707, sodium lauryl sulfate, modified starch, and diatomaceous earth, wherein the mass percentage of DS707 in the adjuvant is 10-20%, the mass percentage of sodium lauryl sulfate in the adjuvant is 15-25%, the mass percentage of modified starch in the adjuvant is 10-25%, and the balance is diatomaceous earth.

9. Use of the herbicide according to any one of claims 1 to 5, 7 to 8, characterized in that: The herbicide is applied in alfalfa fields to control weeds in Chenopodiaceae, Amaranthaceae and Gramineae.

10. The use according to claim 9, characterized in that The application rate of the herbicide is 200-500mL / hm 2 .

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