Preparation method of strigose hydrangea juvenile leaf extract

Abstract

The invention belongs to the technical field of plant extraction, and particularly relates to a preparation method of a strigose hydrangea juvenile leaf extract. According to the present invention, by using the mixed bacterial powder and by using the conditions which are not beneficial to the strain breeding, the strain can effectively decompose the sweet tea during the breeding process, and the decomposition of the internal effective substances can be avoided, and the impurity can be effectively removed through the composite film in the filtration device so as to achieve the effect of the purification; it is guaranteed that the purity of the internal extract is effectively improved, and part of fine impurities can be effectively removed through the mixed solvent.

Description

Technical Field

[0001] The present invention belongs to the technical field of plant extraction, and particularly relates to a method for preparing a sweet tea extract. Background Art

[0002] Sweet tea contains various substances that are very beneficial to the human body, such as rubusoside, tea polyphenols, flavonoids, etc. After being soaked, sweet tea has a very high sweetness, so it is called sweet tea. The medicinal value of sweet tea has a long history. It is often used as a medicine for clearing heat and detoxifying and calming the nerves and reducing blood pressure, and has health care and therapeutic effects such as lowering blood sugar, lowering blood pressure, and promoting metabolism. It is suitable for patients with diabetes, obesity, etc. to eat, and is widely used in the pharmaceutical and health food industries.

[0003] At present, during the extraction process of sweet tea, turbid impurities or paste-like substances will appear, which are difficult to clarify. It is difficult to remove them by centrifugation or filtration, and the yield of sweet tea extract is easily reduced by the flocculation process. Moreover, the tobacco prepared from the sweet tea extract can taste bitter. At present, the extraction rate of the sweet tea extract extraction process is about 80%.

[0004] Therefore, there is an urgent need to propose a method for making a sweet tea extract, so as to improve the extraction rate of the sweet tea extract and remove the bitter taste of the sweet tea extract at the same time. Summary of the Invention

[0005] The purpose of the present invention is to solve one of the technical problems existing in the above-mentioned prior art. For this reason, a method for preparing a sweet tea extract is provided, so that the yield of the sweet tea extract is greater than or equal to 90%.

[0006] To solve the above technical problems, the technical means adopted by the present invention is: A method for preparing a sweet tea extract, the preparation method comprising the following steps: (1) Take fresh sweet tea leaves, soak them in water for 20 minutes, take them out and dry them in the sun until the water content is 30-35%, put them into a pulverizer for pulverization, and collect the pulverized material; (2) Mix the pulverized material with the mixed bacterial powder in a mass ratio of 10:1, place it at 50-55°C for 2-3 days, and add water accounting for 25% of the mass of the pulverized material every 10-13 hours and stir; (3) After the placement is completed, mix the mixture with the mixed solvent in a mass ratio of 1:7-9, shake for 30 minutes, put it into an extruder for extrusion, and collect the extrusion liquid; (4) Decolorize the extrusion liquid, collect the decolorized liquid, put the decolorized liquid into a filtering device for filtration, concentration, add alcohol for alcohol extraction, and obtain the extract.

[0007] Preferably, in the step (2), the mixed bacterial powder is a mixture of yeast powder and lactic acid bacteria powder in any ratio.

[0008] Preferably, in the step (3), the mixed solvent is a sodium chloride solution with a mass fraction of 1% and a hydrochloric acid solution with a mass fraction of 0.3%.

[0009] Preferably, the filter membranes in the filtering device are a microfiltration membrane and an ultrafiltration membrane from top to bottom.

[0010] Preferably, the alcohol in the step (4) is ethanol. Advantages

[0011] By using the mixed bacterial powder under conditions that are relatively unfavorable for the reproduction of the bacterial species, the present invention enables the bacterial species to effectively decompose the sweet tea during the reproduction process while ensuring that the internal active substances are not decomposed. Secondly, the composite membrane inside the filtering device can effectively remove impurities, ensuring that the purity of the internal extract is effectively improved, and the mixed solvent can effectively remove some fine impurities. Embodiments

[0012] The present invention will be specifically described below through examples. It is necessary to point out here that the following examples are only used to further illustrate the present invention and cannot be construed as limiting the protection scope of the present invention. Some non-essential improvements and adjustments made by those skilled in the art based on the above content of the present invention still fall within the protection scope of the present invention.

[0013] In addition, unless otherwise stated, the raw materials used are commercially available. Examples

[0014] The mixed bacterial powder is composed of yeast powder and lactic acid bacteria powder mixed in any ratio.

[0015] The mixed solvent is a sodium chloride solution with a mass fraction of 1% and a hydrochloric acid solution with a mass fraction of 0.3%.

[0016] A method for preparing a sweet tea extract, the preparation method comprising the following steps: (1) Take fresh sweet tea leaves, soak them in water for 20 minutes, take them out and dry them in the sun until the water content is 30%, put them into a pulverizer for pulverization, and collect the pulverized material; (2) Mix the pulverized material and the mixed bacterial powder in a mass ratio of 10:1, place them at 50 °C for 2 days, add water accounting for 25% of the mass of the pulverized material every 10 hours, and stir at 180 r / min; (3) After the placement is completed, mix the mixture and the mixed solvent in a mass ratio of 1:7, shake for 30 minutes, put them into an extruder for extrusion, and collect the extrusion liquid; (4) Decolorize the extrusion liquid, collect the decolorized liquid, place the decolorized liquid in a filtration device. The filter membranes in the filtration device are a microfiltration membrane and an ultrafiltration membrane from top to bottom. Filter and concentrate, add ethanol for ethanol extraction to obtain the extract.

[0017] The yield of the rubus suavissimus extract is 93.5%. Example

[0018] The mixed bacterial powder is composed of yeast powder and lactic acid bacteria powder mixed in any ratio.

[0019] The mixed solvent is a sodium chloride solution with a mass fraction of 1% and a hydrochloric acid solution with a mass fraction of 0.3%.

[0020] A method for preparing a rubus suavissimus extract, the preparation method comprising the following steps: (1) Take fresh rubus suavissimus leaves, soak them in water for 20 min, take them out and dry them in the sun until the water content is 33%, put them into a pulverizer for pulverization, and collect the pulverized material; (2) Mix the pulverized material with the mixed bacterial powder according to a mass ratio of 10:1, place it at 53 °C for 2 days, add water with a mass of 25% of the pulverized material every 12 h, and stir at 180 r / min; (3) After the placement is completed, mix the mixture with the mixed solvent according to a mass ratio of 1:8, shake for 30 min, put it into an extruder for extrusion, and collect the extrusion liquid; (4) Decolorize the extrusion liquid, collect the decolorized liquid, place the decolorized liquid in a filtration device. The filter membranes in the filtration device are a microfiltration membrane and an ultrafiltration membrane from top to bottom. Filter and concentrate, add ethanol for ethanol extraction to obtain the extract.

[0021] The yield of the rubus suavissimus extract is 95.2%. Example

[0022] The mixed bacterial powder is composed of yeast powder and lactic acid bacteria powder mixed in any ratio.

[0023] The mixed solvent is a sodium chloride solution with a mass fraction of 1% and a hydrochloric acid solution with a mass fraction of 0.3%.

[0024] A method for preparing a rubus suavissimus extract, the preparation method comprising the following steps: (1) Take fresh rubus suavissimus leaves, soak them in water for 20 min, take them out and dry them in the sun until the water content is 35%, put them into a pulverizer for pulverization, and collect the pulverized material; (2) Mix the pulverized material with the mixed bacterial powder according to a mass ratio of 10:1, place it at 55 °C for 3 days, add water with a mass of 25% of the pulverized material every 13 h, and stir at 180 r / min; (3)After the placement is completed, mix the mixture with the mixed solvent at a mass ratio of 1:9, shake for 30 min, place it in an extruder for extrusion, and collect the extrusion liquid; (4)Decolorize the extrusion liquid, collect the decolorized liquid, place the decolorized liquid in a filtration device, the filter membrane in the filtration device is a microfiltration membrane and an ultrafiltration membrane from top to bottom, perform filtration and concentration, add ethanol for ethanol extraction to obtain the extract.

[0025] The yield of the rubus suavissimus extract is 94.8%.

[0026] For those skilled in the art, it is obvious that the present invention is not limited to the details of the above exemplary embodiments, and the present invention can be implemented in other specific forms without departing from the spirit or basic characteristics of the present invention. Therefore, from any point of view, the embodiments should be regarded as exemplary and non-limiting. The scope of the present invention is defined by the appended claims rather than the above description. Therefore, all changes falling within the meaning and scope of the equivalent elements of the claims are intended to be included in the present invention.

Claims

1. A method for preparing a sweet tea extract, characterized in that, The preparation method comprises the following steps: (1) Take fresh sweet tea leaves, soak them in water for 20 min, take them out and dry them in the sun until the water content is 30-35%, put them into a pulverizer for pulverization, and collect the pulverized material; (2) Mix the pulverized material with the mixed bacterial powder in a mass ratio of 10:1, place it at 50-55 °C for 2-3 days, add water accounting for 25% of the mass of the pulverized material every 10-13 h, and stir; (3) After the placement is completed, mix the mixture with the mixed solvent in a mass ratio of 1:7-9, shake for 30 min, put it into an extruder for extrusion, and collect the extrusion liquid; (4) Decolorize the extrusion liquid, collect the decolorized liquid, put the decolorized liquid into a filtering device for filtration, concentration, add alcohol for alcohol extraction, and thus obtain the extract.

2. The method for preparing a sweet tea extract according to claim 1, characterized in that, In the step (2), the mixed bacterial powder is composed of yeast powder and lactic acid bacteria powder mixed in any ratio.

3. The method for preparing a sweet tea extract according to claim 1, characterized in that, In the step (3), the mixed solvent is a sodium chloride solution with a mass fraction of 1% and a hydrochloric acid solution with a mass fraction of 0.3%.

4. The method for preparing a sweet tea extract according to claim 1, characterized in that, The filter membrane in the filtering device is a microfiltration membrane and an ultrafiltration membrane from top to bottom.

5. The method for preparing a sweet tea extract according to claim 1, characterized in that, The alcohol in the step (4) is ethanol.